CN109313330B - 执行对样本的超分辨率荧光显微技术成像的装置和方法 - Google Patents

执行对样本的超分辨率荧光显微技术成像的装置和方法 Download PDF

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CN109313330B
CN109313330B CN201780028957.9A CN201780028957A CN109313330B CN 109313330 B CN109313330 B CN 109313330B CN 201780028957 A CN201780028957 A CN 201780028957A CN 109313330 B CN109313330 B CN 109313330B
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light
sample
waveguide assembly
super
waveguide
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Chinese (zh)
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CN109313330A (zh
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B·S·阿卢瓦利亚
M·舒特佩尔茨
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UiT The Arctic University of Norway
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Universität Bielefeld
UiT The Arctic University of Norway
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    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/06—Means for illuminating specimens
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6445—Measuring fluorescence polarisation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G01N21/6458—Fluorescence microscopy
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/16—Microscopes adapted for ultraviolet illumination ; Fluorescence microscopes
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
    • G02B21/365—Control or image processing arrangements for digital or video microscopes
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
    • G02B21/365—Control or image processing arrangements for digital or video microscopes
    • G02B21/367—Control or image processing arrangements for digital or video microscopes providing an output produced by processing a plurality of individual source images, e.g. image tiling, montage, composite images, depth sectioning, image comparison
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B27/00—Optical systems or apparatus not provided for by any of the groups G02B1/00 - G02B26/00, G02B30/00
    • G02B27/56—Optics using evanescent waves, i.e. inhomogeneous waves
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B27/00—Optical systems or apparatus not provided for by any of the groups G02B1/00 - G02B26/00, G02B30/00
    • G02B27/58—Optics for apodization or superresolution; Optical synthetic aperture systems
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B6/00—Light guides; Structural details of arrangements comprising light guides and other optical elements, e.g. couplings
    • G02B6/02—Optical fibres with cladding with or without a coating
    • G02B6/02004—Optical fibres with cladding with or without a coating characterised by the core effective area or mode field radius
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B6/00—Light guides; Structural details of arrangements comprising light guides and other optical elements, e.g. couplings
    • G02B6/02—Optical fibres with cladding with or without a coating
    • G02B6/02042—Multicore optical fibres
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B6/00—Light guides; Structural details of arrangements comprising light guides and other optical elements, e.g. couplings
    • G02B6/10—Light guides; Structural details of arrangements comprising light guides and other optical elements, e.g. couplings of the optical waveguide type
    • G02B6/12—Light guides; Structural details of arrangements comprising light guides and other optical elements, e.g. couplings of the optical waveguide type of the integrated circuit kind
    • G02B6/122—Basic optical elements, e.g. light-guiding paths
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T3/00—Geometric image transformations in the plane of the image
    • G06T3/40—Scaling of whole images or parts thereof, e.g. expanding or contracting
    • G06T3/4053—Scaling of whole images or parts thereof, e.g. expanding or contracting based on super-resolution, i.e. the output image resolution being higher than the sensor resolution
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B6/00—Light guides; Structural details of arrangements comprising light guides and other optical elements, e.g. couplings
    • G02B6/10—Light guides; Structural details of arrangements comprising light guides and other optical elements, e.g. couplings of the optical waveguide type
    • G02B6/12—Light guides; Structural details of arrangements comprising light guides and other optical elements, e.g. couplings of the optical waveguide type of the integrated circuit kind
    • G02B2006/12083—Constructional arrangements
    • G02B2006/12102—Lens

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  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Optics & Photonics (AREA)
  • Analytical Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Multimedia (AREA)
  • Health & Medical Sciences (AREA)
  • Computer Vision & Pattern Recognition (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Theoretical Computer Science (AREA)
  • Microelectronics & Electronic Packaging (AREA)
  • Microscoopes, Condenser (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Optical Measuring Cells (AREA)
CN201780028957.9A 2016-04-12 2017-04-12 执行对样本的超分辨率荧光显微技术成像的装置和方法 Active CN109313330B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB1606268.9A GB2549298B (en) 2016-04-12 2016-04-12 Super-resolution imaging
GB1606268.9 2016-04-12
PCT/GB2017/051034 WO2017178823A1 (en) 2016-04-12 2017-04-12 Super-resolution imaging

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Publication Number Publication Date
CN109313330A CN109313330A (zh) 2019-02-05
CN109313330B true CN109313330B (zh) 2021-06-15

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US (1) US10983326B2 (enExample)
EP (1) EP3443401B1 (enExample)
JP (1) JP6997928B2 (enExample)
KR (1) KR102409738B1 (enExample)
CN (1) CN109313330B (enExample)
AU (1) AU2017248718B2 (enExample)
CA (1) CA3020529C (enExample)
DK (1) DK3443401T3 (enExample)
ES (1) ES2929874T3 (enExample)
GB (1) GB2549298B (enExample)
WO (1) WO2017178823A1 (enExample)

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EP3839483B1 (en) * 2019-12-19 2023-07-26 Imec VZW An illuminator, an imaging apparatus and a system
KR20220138386A (ko) * 2020-01-14 2022-10-12 퀀텀-에스아이 인코포레이티드 도파관 여기 균일성
CN111722392B (zh) * 2020-07-22 2021-06-08 南京大学 一种基于超构透镜阵列的大视场集成显微成像装置
KR102654655B1 (ko) 2020-10-20 2024-04-05 한양대학교 산학협력단 초고해상도 형광 현미경 이미징 방법 및 초고해상도 형광 현미경 이미징 장치
KR102619825B1 (ko) 2020-10-20 2024-01-03 한양대학교 산학협력단 초고해상도 형광 현미경용 이미징 버퍼 용액 및 이를 이용한 초고해상도 형광 현미경 이미징 방법
CN113031243B (zh) * 2021-03-26 2021-08-31 杭州辰景光电科技有限公司 一种基于波导片的反射式片上数字全息显微装置
DE102021131952A1 (de) * 2021-12-03 2023-06-07 Interherence GmbH Optisches Modul
US12536611B2 (en) * 2021-12-08 2026-01-27 Bar Ilan University Method and system for super-resolved imaging
EP4220269A1 (en) * 2022-01-27 2023-08-02 Abbelight Light imaging device
KR102742598B1 (ko) * 2022-02-10 2024-12-16 한양대학교 산학협력단 형광현미경 또는 초고해상도 형광현미경을 이용한 용매의 수소 결합 센싱 방법
CN116297377B (zh) * 2023-05-22 2023-07-28 深圳高性能医疗器械国家研究院有限公司 单分子荧光检测芯片及其制备方法、单分子荧光检测仪

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CN102928384A (zh) * 2012-10-24 2013-02-13 浙江大学 基于微波导的超分辨显微成像方法和装置

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Publication number Publication date
JP2019516140A (ja) 2019-06-13
JP6997928B2 (ja) 2022-01-18
US20190129161A1 (en) 2019-05-02
EP3443401B1 (en) 2022-07-20
AU2017248718B2 (en) 2021-07-15
GB2549298B (en) 2022-02-02
WO2017178823A1 (en) 2017-10-19
US10983326B2 (en) 2021-04-20
AU2017248718A1 (en) 2018-10-25
KR20180130567A (ko) 2018-12-07
EP3443401A1 (en) 2019-02-20
CA3020529C (en) 2023-10-17
CA3020529A1 (en) 2017-10-19
KR102409738B1 (ko) 2022-06-15
DK3443401T3 (da) 2022-10-24
CN109313330A (zh) 2019-02-05
GB2549298A (en) 2017-10-18
ES2929874T3 (es) 2022-12-02

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