CN109295135A - Active compound oligopeptides liquid and its preparation method and application - Google Patents
Active compound oligopeptides liquid and its preparation method and application Download PDFInfo
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- CN109295135A CN109295135A CN201811080645.5A CN201811080645A CN109295135A CN 109295135 A CN109295135 A CN 109295135A CN 201811080645 A CN201811080645 A CN 201811080645A CN 109295135 A CN109295135 A CN 109295135A
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- 230000036737 immune function Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 238000001802 infusion Methods 0.000 description 1
- 230000010354 integration Effects 0.000 description 1
- 229940017800 lactobacillus casei Drugs 0.000 description 1
- 230000003902 lesion Effects 0.000 description 1
- 239000007791 liquid phase Substances 0.000 description 1
- 230000003908 liver function Effects 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 229960000274 lysozyme Drugs 0.000 description 1
- 235000010335 lysozyme Nutrition 0.000 description 1
- 239000004325 lysozyme Substances 0.000 description 1
- 238000003754 machining Methods 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 231100000956 nontoxicity Toxicity 0.000 description 1
- 239000002674 ointment Substances 0.000 description 1
- 231100000418 oral toxicity Toxicity 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 230000001151 other effect Effects 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 239000012071 phase Substances 0.000 description 1
- COLNVLDHVKWLRT-UHFFFAOYSA-N phenylalanine Natural products OC(=O)C(N)CC1=CC=CC=C1 COLNVLDHVKWLRT-UHFFFAOYSA-N 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
- 210000002307 prostate Anatomy 0.000 description 1
- 150000003233 pyrroles Chemical class 0.000 description 1
- 210000004994 reproductive system Anatomy 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000029058 respiratory gaseous exchange Effects 0.000 description 1
- 230000000552 rheumatic effect Effects 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 230000035936 sexual power Effects 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 150000003505 terpenes Chemical class 0.000 description 1
- 235000007586 terpenes Nutrition 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 231100000167 toxic agent Toxicity 0.000 description 1
- 239000003053 toxin Substances 0.000 description 1
- 231100000765 toxin Toxicity 0.000 description 1
- 229940096998 ursolic acid Drugs 0.000 description 1
- PLSAJKYPRJGMHO-UHFFFAOYSA-N ursolic acid Natural products CC1CCC2(CCC3(C)C(C=CC4C5(C)CCC(O)C(C)(C)C5CCC34C)C2C1C)C(=O)O PLSAJKYPRJGMHO-UHFFFAOYSA-N 0.000 description 1
- 210000001835 viscera Anatomy 0.000 description 1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/38—Other non-alcoholic beverages
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/18—Peptides; Protein hydrolysates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Life Sciences & Earth Sciences (AREA)
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- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- Polymers & Plastics (AREA)
- Food Science & Technology (AREA)
- Nutrition Science (AREA)
- Wood Science & Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Microbiology (AREA)
- General Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Mycology (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The present invention relates to health product technology fields, specifically provide a kind of active compound oligopeptides liquid and its preparation method and application.The active compound oligopeptides liquid contains oligopeptides, amino acid, selenium, Thick many candies, total triterpene, solvent；In the active compound oligopeptides liquid, with oligopeptides total amount calculating, molecular weight is not less than 96% in 1000 dalton oligopeptides content below.Active compound oligopeptides liquid of the invention, oligopeptides total amount middle-molecular-weihydroxyethyl reaches 96% or more in 1000 dalton oligopeptides content below, is easily absorbed and utilized by the body, and other compositions are highly beneficial to human body, therefore is suitble to promote and apply.
Description
Technical field
The invention belongs to health product technology fields, more particularly to a kind of active compound oligopeptides liquid and preparation method thereof
And application.
Background technique
Dietotherapy has long history in China, and food is made for the animal-plant material for generalling use integration of drinking and medicinal herbs or drink supplies
Patient takes, and achievees the effect that maintenance.Since it controls feeding combination, the characteristics of long-term nontoxicity, by the high praise of ancient Chinese medicine doctor, and it is long
It has been widely used since phase by people.
Absorption of human body protein is mainly absorbed in the form of small peptide, but according to the product of traditional machining processes acquisition
Molecular weight is greater than 5000 dalton, is not easy to absorb after edible and utilize.For this reason, it is necessary to research and develop a kind of new dietetic food
Or drink.
Summary of the invention
The purpose of the present invention is to provide a kind of active compound oligopeptides liquid and preparation method thereof, it is intended to solve existing
Since molecular weight is greater than the problems such as 5000 dalton are without easily absorbed by the body and utilization in the molecular peptide contained in dietetic food.
Another object of the present invention is to provide a kind of dietotherapy drink and a kind of peptide culture mediums.
The present invention is implemented as follows:
A kind of active compound oligopeptides liquid, at least containing oligopeptides, amino acid, selenium, Thick many candies, total triterpene, active bacteria,
Auxin, pyrroloquinoline quinone, vitamin, unsaturated fatty acid, flavonoids, iodine, lecithin, linolenic acid, superoxide dismutase,
Solvent；
In the active compound oligopeptides liquid, with the oligopeptides total amount calculating, molecular weight 1000 dalton with
Under oligopeptides content be not less than 96%.
And a kind of preparation method of active compound oligopeptides liquid, it at least includes the following steps:
Animal protein and vegetable protein are subjected to mixing treatment, obtain the first mixed material；
Deionized water and honey are added into first mixed material, mixes to uniform, the first mixed liquor of acquisition；
First mixed liquor is placed in installation for fermenting and carries out fermentation process according to following fermenting procedure:
First stage: 20~30 DEG C, normal pressure Anaerobic Treatment, 45~60h of time；
The material that first stage is fermented is centrifuged, and obtains the first concentrate and first dregs of a decoction；
Second stage: it after first dregs of a decoction are mixed with water, is carried out at normal pressure anaerobism under conditions of being placed in 40~60 DEG C
Reason, 45~60h of time；
The material that second stage is fermented is centrifuged, and obtains the second concentrate and second dregs of a decoction；
Add boiling to boil second dregs of a decoction, is centrifugated, obtains third concentrate；
First concentrate, the second concentrate, third concentrate are placed in installation for fermenting together, normal temperature and pressure anaerobism
Storage at least 360 days, obtains active compound oligopeptides liquid.
Correspondingly, a kind of dietotherapy drink, the dietotherapy drink contain active compound oligopeptides liquid as described above or
Person contains the active compound oligopeptides liquid being prepared using preparation method as described above.
A kind of peptide culture medium, the peptide culture medium contain active compound oligopeptides liquid as described above or containing adopting
The active compound oligopeptides liquid being prepared with preparation method as described above.
Beneficial effects of the present invention are as follows:
Compared with the existing technology, active compound oligopeptides liquid provided by the invention, in oligopeptides total amount, molecular weight exists
1000 dalton oligopeptides content below reaches 96% or more, is easily absorbed and utilized by the body；Trace elements of selenium can prevent
And cancer is resisted, mitigate pain or optimal antioxidant, redox reaction generates in energy balanced human's metabolic process
Free radical is simultaneously effectively purged, to prevent damage of the free radical to human organism, it is therefore prevented that lipoids generates peroxidating, human body
Nucleic acid and protein synthesis are reduced, and repair function is low, delaying cell aging, adjusts endocrine, are helped yin-Yang circulation, are put down
Weigh physical function.
The preparation method of active compound oligopeptides liquid provided by the invention obtains fermentation liquid by fermentation, and to obtaining
The fermentation liquid obtained is fermented again, and the effective component in animal/vegetable protein is made to obtain more thorough release, is obtained to have and be lived
Property compound oligopeptides liquid, there is good activity and stability by this method and the compound small molecule oligopeptide that obtains, can be with
It saves and never degenerates for a long time, and oligopeptides middle-molecular-weihydroxyethyl is high less than the oligopeptides content of 1000 dalton, is conducive to be made and be convenient for
Absorption of human body and the food or drink utilized.
Dietotherapy drink provided by the invention, due to containing above-mentioned active compound oligopeptides liquid, molecular weight is less than 1000
The oligopeptides content of dalton is high, is convenient for absorption of human body and utilization, and also beneficial to human body containing amino acid, selenium etc. in the drink
Ingredient, be able to suppress platelet aggregation, prevent thrombosis；It can prevent and resist cancer, mitigate pain, prevent prostate
Lesion；And vitamin can prevent heart disease, enhance sexual performance, have inoxidizability, can aoxidize in balanced human's metabolic process
The free radical of reduction reaction generation is simultaneously effectively purged, to prevent damage of the free radical to human organism, it is therefore prevented that lipoids
It generates peroxidating, human nucleic acid and protein synthesis to reduce, repair function is low, delaying cell aging；Endocrine is adjusted, is helped
Yin-Yang circulation, balances physical function.
Peptide culture medium provided by the invention, it is long since the peptide culture medium contains above-mentioned active compound oligopeptides liquid
Phase preservation is not layered, and is never degenerated, and when as peptide culture medium, the raw material of peptide to be cultivated is added thereto, by culture, i.e.,
It can get corresponding peptide, the oligopeptides in newly-generated peptide and peptide culture medium mutually acts synergistically, and generates new effect, passes through the peptide
Culture medium can obtain rich and varied oligopeptides.
Specific embodiment
In order to which technical problems, technical solutions and advantageous effects to be solved by the present invention are more clearly understood, below in conjunction with
Embodiment, the present invention will be described in further detail.It should be appreciated that specific embodiment described herein is only used to explain
The present invention is not intended to limit the present invention.
Explanation of nouns:
Oligopeptides, also referred to as small peptide, oligopeptide, small active peptides, are a kind of classification of polypeptide, and molecular weight section generally exists
For 1000 dalton hereinafter, be generally made of 2~9 amino acid, be more than is known as polypeptide, and amino acid is more than 50 or more more
Peptide is known as protein.
The present invention provides a kind of active compound oligopeptides liquid, at least contains oligopeptides, amino acid, selenium, Thick many candies, total three
Terpene, solvent；
In the active compound oligopeptides liquid, with the oligopeptides total amount calculating, molecular weight 1000 dalton with
Under oligopeptides content be not less than 96%.
Preferably, in the of the invention every kilogram active compound oligopeptides liquid, the content of selenium is at least
0.10mg, Thick many candies content be at least 150mg, the content of total triterpene is at least 36mg.Contained selenium has anti-oxidant, anti-
Aging is promoted longevity；Protection is repaired, activating cell, and immunity of organisms is improved；It is removing toxic substances, toxin expelling, anti-radiation；Inhibit tumour；It protects
The shield heart cerebrovascular；Protect liver；Help restores islet function；Improve eyesight, improve memory；Promote reproductive system function；?
Effectively prevent the effects for the treatment of rheumatic ostealgia, breathing, enterogastric diseases etc. play extremely important biological effectiveness.Thick many candies tool
There are anticoagulant, antithrombotic, enhancing immunity of organisms, reduction blood glucose, reduction blood lipid and other effects；And total triterpene is triterpenes chemical combination
Object plays an important role in vivo, and participation forms esters, defence toxic agent, growth hormone, has and inhibits cancer cell
Growth, release histamine are to protect liver function, promote platelet aggregation, reducing blood lipid and anti-inflammatory, activity of fighting against senium and other
The functions such as bioactivity.
Further, in terms of the oligopeptides total amount, molecular weight accounts for 70~75% less than the oligopeptides content of 180 dalton, point
Son amount accounts for 15~20% in the oligopeptides of 180~500 dalton.
In active compound oligopeptides liquid of the invention, every liter of active compound oligopeptides liquid at least contains 0.08g
Amino acid.Specifically, the amino acid is asparatate, threonine, serine, glutamic acid, glycine, alanine, figured silk fabrics
Propylhomoserin, methionine, isoleucine, leucine, tyrosine, phenylalanine, lysine, histidine, arginine, proline, color ammonia
At least one of acid, cystine.
In the active compound oligopeptides liquid of above-mentioned offer, active bacteria, auxin, pyrroles's quinoline can also be contained
At least one of quinone, vitamin, unsaturated fatty acid, flavonoids, iodine, lecithin, linolenic acid, superoxide dismutase.
Specifically, the active bacteria is Inonotus obliquus, bacillus licheniformis, the false unicellular bacterium of thermophilic strain, Lactobacillus casei, oxygen
Change grape bacillus Asia and aoxidizes subspecies, Lactobacillus Jensenii, left lactic acid bacillus, Bacillus megatherium, Propionibacterium, goat grape
Bacillus, lactobacillus curvatus, oxidation grape bacillus, lactic acid bacteroid, Lactobacillus brevis, the false unicellular bacterium of food acid, the cruel section bacillus of cream, meat
Staphylococcus, raw skill move glue bacterium, pichia farinose, saccharomyces cerevisiae, Karst brettanomyce, fruit wine fission yeast, lysozyme
At least one of.
Pyrroloquinoline quinone is the necessary factor of human development, can stimulate the growth of human body cell, and especially human activin B is thin
Born of the same parents, T cell are allowed to generate antibody, improve the immune function of human body.
Above-mentioned active compound oligopeptides liquid, it is still active after half a day is boiled in 100 DEG C of boilings, and in acidity
Or alkalinity can cultivate other peptides, therefore have growth activity.
Correspondingly, for the present invention on the basis of providing active compound oligopeptides liquid, still further providing this has work
The preparation method of the compound oligopeptides liquid of property.
One of preparation method at least includes the following steps:
Animal protein and vegetable protein are subjected to mixing treatment, obtain mixed material；
Deionized water and honey are added into the mixed material, mixes to uniform, acquisition mixed liquor；
The mixed liquor is placed in installation for fermenting and carries out fermentation process according to following fermenting procedure:
First stage: 20~30 DEG C, normal pressure Anaerobic Treatment, 45~60h of time；
The material that first stage is fermented is centrifuged, and obtains the first concentrate and first dregs of a decoction；
Second stage: it after first dregs of a decoction are mixed with water, is carried out at normal pressure anaerobism under conditions of being placed in 40~60 DEG C
Reason, 45~60h of time；
The material that second stage is fermented is centrifuged, and obtains the second concentrate and second dregs of a decoction；
Add boiling to boil second dregs of a decoction, is centrifugated, obtains third concentrate；
First concentrate, the second concentrate, third concentrate are placed in installation for fermenting together, normal temperature and pressure anaerobism
Storage at least 360 days, obtains active compound oligopeptides liquid.
The preparation method of the compound oligopeptides liquid active to this does more detailed explanation explanation below.
Preferably, in parts by weight, the feed ratio of the animal protein and vegetable protein is (3~5): (40~50).
Within the scope of the ingredient proportion, the oligopeptides molecular weight of acquisition is relatively high less than the content of 1000 dalton.
Preferably, the animal protein be in tortoise albumen, turtle proteins, Chicken Albumin, pig blood, Fish protein etc. at least
It is a kind of.Preferably, the vegetable protein is ginseng, ganoderma lucidum, gynostemma pentaphylla, myotonin, buckwheat, malt, jujube, hawthorn, Radix Angelicae Sinensis, life
At least one of ground, Poria cocos, Radix Astragali, fructus lycii, black rice, Semen sesami nigrum, mulberries, Radix Glycyrrhizae, agaric, orange etc..
It is further preferable that including 2 parts of full tortoise in the mixed material, complete according to weight part ratio in one embodiment
2 parts of soft-shelled turtle, 30 parts of full egg, 20 parts of pig blood, 10 parts of fish, 8 parts of ginseng, 9 parts of ganoderma lucidum, 40 parts of gynostemma pentaphylla, 100 parts of myotonin, Qiao
80 parts of wheat, 60 parts of malt, 30 parts of jujube, 10 parts of mountain slag, 7 parts of Radix Angelicae Sinensis, 10 parts of Radix Rehmanniae, 8 parts of Poria cocos, 5 parts of Radix Astragali, 20 parts of fructus lycii,
50 parts of black rice, 10 parts of Semen sesami nigrum, 40 parts of mulberries, 6 parts of Radix Glycyrrhizae, 1 part of agaric, 10 parts of orange.It is equal for above-mentioned tortoise, soft-shelled turtle, fish
I.e. use is cleaned for fresh and alive kill, egg is then cleaned up with shell and be can be used, and Chinese medicine ginseng, ganoderma lucidum, gynostemma pentaphylla, adlay
Rice, buckwheat, malt, jujube, hawthorn, Radix Angelicae Sinensis, Radix Rehmanniae, Poria cocos, Radix Astragali, fructus lycii, black rice, Semen sesami nigrum, mulberries, Radix Glycyrrhizae, agaric are then
For dry material, the fruit such as orange are added with fresh fruit.
Preferably, the deionized water is 8~15 times of the mixed material.Excessive deionized water, which is added, to be made
Object albumen and vegetable protein are adequately impregnated, the subsequent solvent as active compound oligopeptides liquid.
Preferably, the parts by weight of the honey are 80~120 parts, and parts by weight here are relative to animal protein and plant
For Tot Prot part, that is, 80~120 parts of honey are added in every 200~220 parts of mixed materials.Honey is during the fermentation
Sugar required for microorganism is provided.
The time of storage is longer, and the oligopeptides liquid active constituent of acquisition and the stabilization of enzyme are higher, and entire oligopeptides liquid is also more stable,
To finally fermenting, obtained active compound oligopeptides liquid can also carry out sterilization treatment.
Preparation method of the present invention obtains active compound oligopeptides liquid as a result,.Due to the active compound oligopeptides
In oligopeptides total amount contained in liquid, molecular weight reaches 96% or more in 1000 dalton oligopeptides content below, easily by human body
It absorbs and utilizes；Active bacteria can effectively inhibit platelet aggregation, prevent thrombosis；And trace elements of selenium can prevent and support
Anticancer mitigates pain or optimal antioxidant, the freedom that redox reaction generates in energy balanced human's metabolic process
Base is simultaneously effectively purged, to prevent damage of the free radical to human organism, it is therefore prevented that lipoids generates peroxidating, human nucleic acid
It synthesizes and reduces with protein, repair function is low, delaying cell aging, adjusts endocrine, helps yin-Yang circulation, balances body
Body function.It therefore can be using the active compound oligopeptides liquid as the ingredient of dietotherapy drink.
Accordingly, the present invention provides a kind of dietotherapy drink, has in the ingredient of the dietotherapy drink containing provided by the invention
Active compound oligopeptides liquid.
The present invention furthermore provides a kind of peptide culture medium, and the peptide culture medium is provided by the invention active answers
Close oligopeptides liquid.The peptide culture medium can be used to cultivate other peptides, suitable for animal peptide, the extraction of plant peptide.Such as to the peptide culture
Sea cucumber is added in base, sea cucumber peptide can be obtained through culture；Ox bone is added into the peptide culture medium can obtain ox bone peptide；To the peptide
Beef is added in culture medium can obtain beef peptide；Noni fruit is added into the peptide culture medium can obtain Noni fruit peptide；To this
Natto is added in peptide culture medium can obtain natto peptide；Sea-buckthorn is added into the peptide culture medium can obtain sea-buckthorn peptide；To the peptide
Grain wine is added in culture medium can cultivate acquisition wine peptide etc..By peptide culture medium of the invention, can be mentioned in 0 DEG C or more culture
Other peptides are taken, solve the problems, such as that existing peptide can not be cultivated at low temperature.
Technical solution in order to better illustrate the present invention, is illustrated combined with specific embodiments below.
Embodiment 1
A kind of preparation method of active compound oligopeptides liquid.The preparation method comprises the following steps:
(1) raw material components prepare: according to weight part ratio, weighing 2 parts of full tortoise, 2 parts of full soft-shelled turtle, 30 parts of full egg, pig blood
20 parts, 10 parts of fish are used as animal protein；
8 parts of ginseng, 9 parts of ganoderma lucidum, 40 parts of gynostemma pentaphylla, 100 parts of myotonin, 80 parts of buckwheat, 60 parts of malt, 30 parts of jujube, mountain
10 parts of slag, 7 parts of Radix Angelicae Sinensis, 10 parts of Radix Rehmanniae, 8 parts of Poria cocos, 5 parts of Radix Astragali, 20 parts of fructus lycii, 50 parts of black rice, 10 parts of Semen sesami nigrum, mulberries 40
Part, 6 parts of Radix Glycyrrhizae, 1 part of agaric, 10 parts of orange be used as vegetable protein.
(2) animal protein and vegetable protein obtained step (1) carries out mixing treatment, and parts by weight are added thereto and are
10 times of deionized water of the animal protein and vegetable protein total amount and 160 parts of honey, are stirred until homogeneous and are placed on enzymatic vessel
According to following fermenting procedure carry out fermentation process:
First stage: 25 DEG C, normal pressure Anaerobic Treatment, time 50h；Centrifugation point is carried out to the material that the first stage ferments
From obtaining the first concentrate and first dregs of a decoction；
Second stage: first dregs of a decoction are mixed with water, the water of addition floods first dregs of a decoction, is placed in 50 DEG C
Under the conditions of carry out normal pressure Anaerobic Treatment, time 50h.
(3) material to ferment to second stage in step (2) is carried out from centrifuge separation, obtains the second concentrate and the
Two dregs of a decoction；
It after second dregs of a decoction are mixed with deionized water, boils, keeps 30min, centrifuge separation obtains third concentrate, gives up
Slag abandons.
(4) after the first concentrate, the second concentrate, the third concentrate obtained step (3) mixes, it is transferred to enzymatic vessel
In, it is stored 360 days under normal temperature and pressure anaerobic environment, takes out sterilization treatment, obtain active compound oligopeptides liquid.
The product obtained for verifying is active compound oligopeptides liquid really, and the inspection that corresponding ingredient is carried out to it is surveyed
Examination.
1. oligopeptides molecular weight distribution is tested
Tester: high performance liquid chromatograph；Test foundation: GB/T22492-2008 soy peptide powder；Inspection number and title:
NLC180306037Y1 green grass collects peptide liquid；Detect unit: Institute of Analysis of Southern Yangtze University；Specific test result is as shown in table 1.
1 oligopeptides molecular weight distribution of table
Show product relative molecular weight that embodiment 1 obtains in the comparison of ingredients of 0~1000 dalton by 1 test result of table
It is more, reach 23%, it is believed that be the mixture of oligopeptides and amino acid, essentially amino of the relative molecular weight less than 180 dalton
Acid.
2. aminoacid ingredient detects
Detecting instrument: Hitachi's L-8900 amino-acid analyzer etc.；Examination criteria: GB5009.124-2016；Inspection number and
Title: J01889701 green grass collects peptide liquid；Detect unit: spectrum Buddhist nun tests group Shanghai Co., Ltd.
2 aminoacid ingredient of table and its content (g/100mL)
3. the detection of the ingredients such as Thick many candies, total triterpene
Examination criteria: " health food functional component detection method ", NY/T1676-2008；Detect unit: spectrum Buddhist nun detects collection
Shanghai Co., Ltd of group, testing result are as shown in table 3.
The testing result of 3 selenium of table, Thick many candies, total triterpene
| Detection project | Testing result | With reference to method |
| Thick many candies (in terms of glucan) mg/g | 0.277 | " health food functional component detection method " |
| Total triterpene (in terms of ursolic acid) mg/100g | 128 | " health food functional component detection method " |
| Selenium (mg/kg) | 0.12 | GB5009.93-2017 |
4. animal toxicity is tested
According to GB15193.3-2014 " test of national food safety standard polarity Oral toxicity " polarity toxicity grading standard.
Experimental animal uses credit number: SYXK (Shan) 2016-007
Animal species strain: kunming mice
Buy the date: on 01 30th, 2018
Animal origin: the long-living Biotechnology Ltd. in Liaoning (experimental animal production licence number: SCXK (the Liao Dynasty)
2015-0001)。
Configure activated oligomeric oligopeptide solution, solvent: pure water.Activated oligomeric oligopeptides 15.019g is weighed, is settled to pure water
30mL is mixed spare.
Test method: according to trial test as a result, formal test uses limitation method, dose design 10000mg/kg.bw.
Test is fifty-fifty using 20 female heaps of animal；Using the primary oral gastric infusion of isometric(al) 20mL/kg.bw.Animal fasting 5 before being administered
Hour, not water restriction.Continue fasting 1h to ointment.
And the biologically active peptide obtained through the invention with the mode of biological enzymolysis, it is edible can after do not need liver synthesis,
Directly absorbed without digestive system, be directly entered cell, can synthetic cell, and adjust the function of cell, make the function of beneficial
Effect obtains supernormal performance.
Observation index: observing the poisoning manifestations and death condition of animal immediately after administration, at least observation daily thereafter is primary,
14d is observed continuously.During test, record animal weight, animal dead number and death time weekly, and to dead animal and observation
Expire and put to death animal and be autopsied, visually observe tissue or internal organs have it is without exception.
4 animal subject clinical symptoms of table and death condition summarize data
5 animal subject body weights of table summarize data
From 4 table 5 of table: animal subject has no any poisoning symptom and dead disease after contamination in 14 days observation time limits
Shape, and each gender the weight of animals shows a increasing trend, observation puts to death animal after expiring and carries out dissect, and it is different not find that organ or tissue has
Often.It can determine that activated oligomeric oligopeptides of the invention to the acute oral LD of kunming mice50> 10000mg/kgbw.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all in essence of the invention
Made any modifications, equivalent replacements, and improvements etc., should all be included in the protection scope of the present invention within mind and principle.
Claims (10)
1. a kind of active compound oligopeptides liquid, which is characterized in that contain oligopeptides, amino acid, selenium, Thick many candies, total triterpene, molten
Agent；
In the active compound oligopeptides liquid, by the oligopeptides total amount be 100% in terms of, molecular weight 1000 dalton with
Under oligopeptides content be not less than 96%.
2. active compound oligopeptides liquid as described in claim 1, which is characterized in that every kilogram described active to answer
It closes the selenium, Thick many candies, total triterpene contained in oligopeptides liquid and is at least 0.10mg, 150mg, 36mg respectively.
3. active compound oligopeptides liquid as described in claim 1, which is characterized in that the amino acid is asparagine
Acid, threonine, serine, glutamic acid, glycine, alanine, valine, methionine, isoleucine, leucine, tyrosine, benzene
At least one of alanine, lysine, histidine, arginine, proline, tryptophan, cystine.
4. active compound oligopeptides liquid as described in claim 1, which is characterized in that the active compound oligopeptides
Also contain active bacteria, auxin, pyrroloquinoline quinone, vitamin, unsaturated fatty acid, flavonoids, iodine, lecithin, linolenic acid, surpass
At least one of superoxide dismutase.
5. a kind of preparation method of such as described in any item active compound oligopeptides liquid of Claims 1 to 4, feature exist
In at least including the following steps:
Animal protein and vegetable protein are subjected to mixing treatment, obtain mixed material；
Deionized water and honey are added into the mixed material, mixes to uniform, acquisition mixed liquor；
The mixed liquor is placed in installation for fermenting and carries out fermentation process according to following fermenting procedure:
First stage: 20~30 DEG C, normal pressure Anaerobic Treatment, 45~60h of time；
The material that first stage is fermented is centrifuged, and obtains the first concentrate and first dregs of a decoction；
Second stage: after first dregs of a decoction are mixed with water, carrying out normal pressure Anaerobic Treatment under conditions of being placed in 40~60 DEG C, when
Between 45~60h；
The material that second stage is fermented is centrifuged, and obtains the second concentrate and second dregs of a decoction；
Add boiling to boil second dregs of a decoction, is centrifugated, obtains third concentrate；
First concentrate, the second concentrate, third concentrate are placed in installation for fermenting together, the storage of normal temperature and pressure anaerobism
At least 360 days, obtain active compound oligopeptides liquid.
6. the preparation method of active compound oligopeptides liquid as claimed in claim 5, which is characterized in that according to parts by weight
Meter, the animal protein and vegetable protein feed ratio are (3~5): (40~50)；
And/or the animal protein is at least one of tortoise albumen, turtle proteins, pig blood, Fish protein, Chicken Albumin；
And/or the vegetable protein be ginseng, ganoderma lucidum, gynostemma pentaphylla, myotonin, buckwheat, malt, jujube, hawthorn, Radix Angelicae Sinensis, Radix Rehmanniae,
At least one of Poria cocos, Radix Astragali, black rice, Semen sesami nigrum, mulberries, Radix Glycyrrhizae, fructus lycii, orange, agaric.
7. the preparation method of active compound oligopeptides liquid as claimed in claim 5, which is characterized in that according to parts by weight
Than, in the mixed material comprising 2 parts of full tortoise, 2 parts of full soft-shelled turtle, 30 parts of full egg, 20 parts of pig blood, 10 parts of fish, 8 parts of ginseng,
9 parts of ganoderma lucidum, 40 parts of gynostemma pentaphylla, 100 parts of myotonin, 80 parts of buckwheat, 60 parts of malt, 30 parts of jujube, 10 parts of mountain slag, 7 parts of Radix Angelicae Sinensis, life
10 parts of ground, 8 parts of Poria cocos, 5 parts of Radix Astragali, 50 parts of black rice, 10 parts of Semen sesami nigrum, 40 parts of mulberries, 6 parts of Radix Glycyrrhizae, 20 parts of fructus lycii, 1 part of agaric,
10 parts of orange.
8. the preparation method of active compound oligopeptides liquid as claimed in claim 5, which is characterized in that the deionized water
It is 8~15 times of the mixed material；
And/or the weight part ratio of the parts by weight of the honey and the mixed material is (4~6): (10~11).
9. a kind of dietotherapy drink, which is characterized in that the dietotherapy drink contains to be had as Claims 1 to 4 is described in any item
Active compound oligopeptides liquid has work containing what is be prepared using the described in any item preparation methods of claim 5~8
The compound oligopeptides liquid of property.
10. a kind of peptide culture medium, which is characterized in that the peptide culture medium contains to be had as Claims 1 to 4 is described in any item
Active compound oligopeptides liquid has work containing what is be prepared using the described in any item preparation methods of claim 5~8
The compound oligopeptides liquid of property.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110846230A (en) * | 2019-12-06 | 2020-02-28 | 舒培健康科学有限公司 | Preparation method of composite peptide |
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| CN107455698A (en) * | 2017-09-26 | 2017-12-12 | 大连大学 | A kind of ocean polypeptide spy's dietary seafood for being used to promote intelligence development |
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