CN109295003A - 产生牛妊娠相关糖蛋白特异性单克隆抗体的小鼠杂交瘤细胞株、单克隆抗体、试剂盒及检测 - Google Patents
产生牛妊娠相关糖蛋白特异性单克隆抗体的小鼠杂交瘤细胞株、单克隆抗体、试剂盒及检测 Download PDFInfo
- Publication number
- CN109295003A CN109295003A CN201811051878.2A CN201811051878A CN109295003A CN 109295003 A CN109295003 A CN 109295003A CN 201811051878 A CN201811051878 A CN 201811051878A CN 109295003 A CN109295003 A CN 109295003A
- Authority
- CN
- China
- Prior art keywords
- pag
- monoclonal antibody
- antibody
- detection
- hybridoma cell
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/40—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against enzymes
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/573—Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/577—Immunoassay; Biospecific binding assay; Materials therefor involving monoclonal antibodies binding reaction mechanisms characterised by the use of monoclonal antibodies; monoclonal antibodies per se are classified with their corresponding antigens
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/902—Oxidoreductases (1.)
- G01N2333/906—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.7)
- G01N2333/90605—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.7) acting on the CH-NH2 group of donors (1.4)
- G01N2333/90611—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.7) acting on the CH-NH2 group of donors (1.4) with NAD or NADP as acceptor (1.4.1) in general
- G01N2333/90616—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.7) acting on the CH-NH2 group of donors (1.4) with NAD or NADP as acceptor (1.4.1) in general with a definite EC number (1.4.1.-)
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Urology & Nephrology (AREA)
- Biomedical Technology (AREA)
- Hematology (AREA)
- Medicinal Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- General Physics & Mathematics (AREA)
- Food Science & Technology (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Organic Chemistry (AREA)
- Microbiology (AREA)
- Pathology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Genetics & Genomics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Abstract
Description
Claims (10)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201811051878.2A CN109295003B (zh) | 2018-09-10 | 2018-09-10 | 产生牛妊娠相关糖蛋白特异性单克隆抗体的小鼠杂交瘤细胞株、单克隆抗体、试剂盒及检测 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201811051878.2A CN109295003B (zh) | 2018-09-10 | 2018-09-10 | 产生牛妊娠相关糖蛋白特异性单克隆抗体的小鼠杂交瘤细胞株、单克隆抗体、试剂盒及检测 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN109295003A true CN109295003A (zh) | 2019-02-01 |
CN109295003B CN109295003B (zh) | 2022-03-04 |
Family
ID=65166803
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201811051878.2A Active CN109295003B (zh) | 2018-09-10 | 2018-09-10 | 产生牛妊娠相关糖蛋白特异性单克隆抗体的小鼠杂交瘤细胞株、单克隆抗体、试剂盒及检测 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN109295003B (zh) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN111351943A (zh) * | 2020-03-04 | 2020-06-30 | 长江师范学院 | 一种基于适配体识别-hcr反应的牛早孕快速检测方法及应用 |
CN114134123A (zh) * | 2021-12-02 | 2022-03-04 | 深圳市易瑞生物技术股份有限公司 | 妊娠相关糖蛋白的单克隆抗体及其在牛早期怀孕检测中的应用 |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101918445A (zh) * | 2007-12-13 | 2010-12-15 | 孟山都技术公司 | 用于早期妊娠诊断的组合物和方法 |
CN105838679A (zh) * | 2016-04-12 | 2016-08-10 | 北京瑞鹰生物技术有限公司 | 牛妊娠相关糖蛋白pag特异性单克隆抗体细胞株及其应用 |
-
2018
- 2018-09-10 CN CN201811051878.2A patent/CN109295003B/zh active Active
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101918445A (zh) * | 2007-12-13 | 2010-12-15 | 孟山都技术公司 | 用于早期妊娠诊断的组合物和方法 |
CN105838679A (zh) * | 2016-04-12 | 2016-08-10 | 北京瑞鹰生物技术有限公司 | 牛妊娠相关糖蛋白pag特异性单克隆抗体细胞株及其应用 |
Non-Patent Citations (1)
Title |
---|
JONATHAN A. GREEN: "The establishment of an ELISA for the detection of pregnancy-associated glycoproteins (PAGs) in the serum of pregnant cows and heifers", 《THERIOGENOLOGY》 * |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN111351943A (zh) * | 2020-03-04 | 2020-06-30 | 长江师范学院 | 一种基于适配体识别-hcr反应的牛早孕快速检测方法及应用 |
CN114134123A (zh) * | 2021-12-02 | 2022-03-04 | 深圳市易瑞生物技术股份有限公司 | 妊娠相关糖蛋白的单克隆抗体及其在牛早期怀孕检测中的应用 |
CN114134123B (zh) * | 2021-12-02 | 2023-05-05 | 深圳市爱医生物科技有限公司 | 妊娠相关糖蛋白的单克隆抗体及其在牛早期怀孕检测中的应用 |
WO2023098215A1 (zh) * | 2021-12-02 | 2023-06-08 | 深圳市爱医生物科技有限公司 | 妊娠相关糖蛋白的单克隆抗体及其在牛早期怀孕检测中的应用 |
Also Published As
Publication number | Publication date |
---|---|
CN109295003B (zh) | 2022-03-04 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN101918445B (zh) | 用于早期妊娠诊断的组合物和方法 | |
DK160108C (da) | Fremgangsmåde og udstyr til direkte eller indirekte påvisning af reaktion mellem et specifikt bindingsmiddel og den tilsvarende acceptorsubstans | |
CN109295003A (zh) | 产生牛妊娠相关糖蛋白特异性单克隆抗体的小鼠杂交瘤细胞株、单克隆抗体、试剂盒及检测 | |
CN102876634B (zh) | 孕马血清促性腺激素双抗体夹心elisa试剂盒 | |
CN111587252B (zh) | 与牛妊娠相关糖蛋白1特异性结合的抗体及其用途 | |
CN103509760B (zh) | 可分泌抗β2-微球蛋白单克隆抗体的杂交瘤细胞及应用 | |
US6787324B2 (en) | Method and apparatus for detecting conception in animals | |
DK169571B1 (da) | Monoklont antistof, fremgangsmåde til dets fremstilling, hybridomcellelinie, der producerer antistoffet, fremgangsmåder til kvantitativ immunologisk bestemmelse af prokollagen-peptid (type III) med antistoffet, samt diagnostisk præparat indeholdende det monoklonale antistof | |
CN107389920A (zh) | 一种检测禽白细胞介素17a含量的方法及其专用试剂盒 | |
Peknicova et al. | Monoclonal antibodies against boar acrosomal antigens labelling undamaged acrosomes of spermatozoa in immunofluorescence test: Monoklonale Antikörper gegen akrosomale Antigene des Ebers | |
WO2023098215A1 (zh) | 妊娠相关糖蛋白的单克隆抗体及其在牛早期怀孕检测中的应用 | |
CN103525769B (zh) | 可分泌抗视黄醇结合蛋白单克隆抗体的杂交瘤细胞及应用 | |
CN110204616A (zh) | 一种抗白色念珠菌烯醇化酶特异性单克隆抗体的制备方法及其应用 | |
CN105838680B (zh) | 一株抗雌三醇特异性单克隆抗体杂交瘤细胞株NaN-2及其应用 | |
CN104730250A (zh) | 一种检测人肾损伤分子的酶联免疫试剂盒 | |
US7179640B2 (en) | Method and apparatus for detecting conception in animals | |
CN112694517B (zh) | 一种人工多肽pl、其抗体及在病理检测中的应用 | |
JP2010139429A (ja) | 腎疾患の検出方法 | |
CN117820434A (zh) | 牛羊妊娠相关糖蛋白的多肽抗原及应用 | |
JPS585661A (ja) | 前立腺酸ホスフアタ−ゼアイソザイムの定量方法 | |
CN110054663A (zh) | Ckap4抗原表位肽、抗原、单克隆抗体及其应用和ckap4检测试纸条 | |
CN115806597A (zh) | 一种鉴定或鉴别新冠突变型抗原的抗体、试剂及方法 | |
MXPA00007527A (en) | Method and apparatus for detecting conception in animals | |
JPH0772148A (ja) | ヒトiv型コラーゲンのサンドイッチ酵素免疫学的定量用試薬 | |
JPH02136745A (ja) | 口腔病原菌に特異的なモノクローナル抗体 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
CB03 | Change of inventor or designer information |
Inventor after: Wang Huiyan Inventor after: Cai Jianhui Inventor after: Hu Chuanmin Inventor after: Xu Song Inventor after: Sun Meiyan Inventor after: Liu Jing Inventor after: Li Yan Inventor before: Cai Jianhui Inventor before: Xu Song Inventor before: Li Yan Inventor before: Hu Chuanmin Inventor before: Sun Meiyan Inventor before: Wang Huiyan Inventor before: Liu Jing |
|
CB03 | Change of inventor or designer information | ||
GR01 | Patent grant | ||
GR01 | Patent grant |