CN109288886A - A kind of extracting method of fenugreek tincture - Google Patents
A kind of extracting method of fenugreek tincture Download PDFInfo
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- CN109288886A CN109288886A CN201811082100.8A CN201811082100A CN109288886A CN 109288886 A CN109288886 A CN 109288886A CN 201811082100 A CN201811082100 A CN 201811082100A CN 109288886 A CN109288886 A CN 109288886A
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- Prior art keywords
- fenugreek
- mixed enzyme
- pectase
- tincture
- added
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/0012—Galenical forms characterised by the site of application
- A61K9/0014—Skin, i.e. galenical aspects of topical compositions
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
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- Health & Medical Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Life Sciences & Earth Sciences (AREA)
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- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
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- Engineering & Computer Science (AREA)
- Dermatology (AREA)
- Alternative & Traditional Medicine (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Medical Informatics (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
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Abstract
The invention discloses a kind of extracting methods of fenugreek tincture, comprising the following steps: a. selects fenugreek, cleans up after weighing, and the plant tissue of faenum graecum is used broken crusher machine;B. it is added mixed enzyme into broken tissue, digests 18-32h, the mixed enzyme includes cellulase and pectase, and the mass ratio of cellulase and pectase is 1:0.5-2;C. a certain amount of mixed enzyme is added in the product obtained to step b, digests 8-16h, and the mixed enzyme includes cellulase and pectase, and the mass ratio of cellulase and pectase is 1:0.5-2;D. a certain proportion of ethanol solution is added in the product obtained to step c, extracts 6-12h, strainer filtering collects extracting solution, as fenugreek tincture.The fenugreek tincture purity is high of the method for the present invention preparation, raw material availability is high, reduces production cost, sufficiently meets the needs of industry, has broad application prospects.
Description
Technical field
The present invention relates to a kind of plant tincture extraction process, the extracting method of specifically a kind of fenugreek tincture.
Background technique
Faenum graecum (Classification system:Trigonella foenum-graecum L.), it is pulse family Trigonella plant Hu Lu
Bar habit claim, also make fenugreek.Annual herb plant.30-80 centimetres high, well developed root system, stem is upright, pinniform trifoliolate leaf,
Petiole is open and flat.Flower is without stalk, and calyx tube shape, corolla yellow-white or faint yellow, ovary is linear, and pod is cylindric, and the long round shape of seed is oval.
The month at florescence 4-7, the fruiting period 7-9 month.Faenum graecum is distributed in the ground such as China, Mediterranean east bank, Himalaya Region, is born in field, road
It is other.Hu Luba, seed are used as medicine, can tonifying kidney and strengthening yang, eliminating the phlegm dehumidifying;Tender stem, leaf can be for vegetable consumption;Stem, leaf or seed shine dry grinding
Steamed wheaten foods makees fumet in powder incorporation flour;Dry herb can disinfest;It is capable of using as feed.
Currently, the method for extracting faenum graecum tincture from faenum graecum is at high cost, the utilization rate of raw material is low, complex process, no
It is able to satisfy industrial needs.
Summary of the invention
The present invention is exactly to solve the above-mentioned problems, to provide a kind of extracting method of fenugreek tincture.
The present invention is implemented according to following technical scheme.
A kind of extracting method of fenugreek tincture, comprising the following steps:
A. fenugreek is selected, is cleaned up after weighing, the plant tissue of faenum graecum is used into broken crusher machine;
B. mixed enzyme is added into broken tissue, digests 18-32h, the mixed enzyme includes cellulase and pectase, fibre
The mass ratio for tieing up plain enzyme and pectase is 1:0.5-2;
C. a certain amount of mixed enzyme is added in the product obtained to step b, digests 8-16h, the mixed enzyme includes cellulose
The mass ratio of enzyme and pectase, cellulase and pectase is 1:0.5-2;
D. a certain proportion of ethanol solution is added in the product obtained to step c, extracts 6-12h, strainer filtering is collected and extracted
Liquid, as fenugreek tincture.
Further, broken tissue and the mass ratio of mixed enzyme are 1:0.01-0.05 in the step b.
Further, the ratio that mixed enzyme is added in the step b and step c is 2:1.
Further, enzymolysis time is for 24 hours in the step b.
Further, enzymolysis time is 12h in the step c.
Further, the ethanol solution that ethanol solution is 25-55% in the step d.
Further, the ethanol solution that ethanol solution is 25% in the step d extracts 8h.
Present invention obtains following beneficial effects.
The fenugreek tincture purity is high of the method for the present invention preparation, raw material availability is high, reduces production cost, sufficiently meets
The needs of industry, have broad application prospects.
Specific embodiment
Further technology explanation is carried out to the present invention referring to embodiment.
Embodiment 1
A kind of extracting method of fenugreek tincture, comprising the following steps:
A. fenugreek is selected, is cleaned up after weighing, the plant tissue of faenum graecum is used into broken crusher machine;
B. mixed enzyme is added into broken tissue, it is broken to organize to be 1:0.01, enzymatic hydrolysis with the mass ratio of mixed enzyme
32h, the mixed enzyme include cellulase and pectase, and the mass ratio of cellulase and pectase is 1:0.5;
C. a certain amount of mixed enzyme is added in the product obtained to step b, the ratio that mixed enzyme is added in step b and step c
For 2:1,8h is digested, the mixed enzyme includes cellulase and pectase, and the mass ratio of cellulase and pectase is 1:0.5;
D. 25% ethanol solution is added in the product obtained to step c, extracts 6h, strainer filtering collects extracting solution, as recklessly
Reed bar tincture.
Embodiment 2
A kind of extracting method of fenugreek tincture, comprising the following steps:
A. fenugreek is selected, is cleaned up after weighing, the plant tissue of faenum graecum is used into broken crusher machine;
B. mixed enzyme is added into broken tissue, it is broken to organize to be 1:0.25, enzymatic hydrolysis with the mass ratio of mixed enzyme
For 24 hours, the mixed enzyme includes cellulase and pectase, and the mass ratio of cellulase and pectase is 1:1;
C. a certain amount of mixed enzyme is added in the product obtained to step b, the ratio that mixed enzyme is added in step b and step c
For 2:1,12h is digested, the mixed enzyme includes cellulase and pectase, and the mass ratio of cellulase and pectase is 1:1;
D. 25% ethanol solution is added in the product obtained to step c, extracts 8h, strainer filtering collects extracting solution, as recklessly
Reed bar tincture.
Embodiment 3
A kind of extracting method of fenugreek tincture, comprising the following steps:
A. fenugreek is selected, is cleaned up after weighing, the plant tissue of faenum graecum is used into broken crusher machine;
B. mixed enzyme is added into broken tissue, it is broken to organize to be 1:0.05, enzymatic hydrolysis with the mass ratio of mixed enzyme
18h, the mixed enzyme include cellulase and pectase, and the mass ratio of cellulase and pectase is 1:2;
C. a certain amount of mixed enzyme is added in the product obtained to step b, the ratio that mixed enzyme is added in step b and step c
For 2:1,16h is digested, the mixed enzyme includes cellulase and pectase, and the mass ratio of cellulase and pectase is 1:2;
D. 55% ethanol solution is added in the product obtained to step c, extracts 12h, strainer filtering collects extracting solution, as
Fenugreek tincture.
Claims (7)
1. a kind of extracting method of fenugreek tincture, which comprises the following steps:
A. fenugreek is selected, is cleaned up after weighing, the plant tissue of faenum graecum is used into broken crusher machine;
B. mixed enzyme is added into broken tissue, digests 18-32h, the mixed enzyme includes cellulase and pectase, fibre
The mass ratio for tieing up plain enzyme and pectase is 1:0.5-2;
C. a certain amount of mixed enzyme is added in the product obtained to step b, digests 8-16h, the mixed enzyme includes cellulose
The mass ratio of enzyme and pectase, cellulase and pectase is 1:0.5-2;
D. a certain proportion of ethanol solution is added in the product obtained to step c, extracts 6-12h, strainer filtering is collected and extracted
Liquid, as fenugreek tincture.
2. a kind of extracting method of fenugreek tincture as described in claim 1, which is characterized in that after being crushed in the step b
Tissue and mixed enzyme mass ratio be 1:0.01-0.05.
3. a kind of extracting method of fenugreek tincture as described in claim 1, which is characterized in that in the step b and step c
The ratio that mixed enzyme is added is 2:1.
4. a kind of extracting method of fenugreek tincture as described in claim 1, which is characterized in that when being digested in the step b
Between for for 24 hours.
5. a kind of extracting method of fenugreek tincture as described in claim 1, which is characterized in that when being digested in the step c
Between be 12h.
6. a kind of extracting method of fenugreek tincture as described in claim 1, which is characterized in that ethyl alcohol is molten in the step d
Liquid is the ethanol solution of 25-55%.
7. a kind of extracting method of fenugreek tincture as claimed in claim 6, which is characterized in that ethyl alcohol is molten in the step d
The ethanol solution that liquid is 25% extracts 8h.
Priority Applications (1)
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CN201811082100.8A CN109288886A (en) | 2018-09-17 | 2018-09-17 | A kind of extracting method of fenugreek tincture |
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CN201811082100.8A CN109288886A (en) | 2018-09-17 | 2018-09-17 | A kind of extracting method of fenugreek tincture |
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CN109288886A true CN109288886A (en) | 2019-02-01 |
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CN201811082100.8A Pending CN109288886A (en) | 2018-09-17 | 2018-09-17 | A kind of extracting method of fenugreek tincture |
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2018
- 2018-09-17 CN CN201811082100.8A patent/CN109288886A/en active Pending
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Application publication date: 20190201 |