CN109287621A - A kind of extender of freezing semen improving milk cow conception rate - Google Patents
A kind of extender of freezing semen improving milk cow conception rate Download PDFInfo
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- CN109287621A CN109287621A CN201811368033.6A CN201811368033A CN109287621A CN 109287621 A CN109287621 A CN 109287621A CN 201811368033 A CN201811368033 A CN 201811368033A CN 109287621 A CN109287621 A CN 109287621A
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- milk cow
- water extract
- conception rate
- extender
- semen
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0205—Chemical aspects
- A01N1/021—Preservation or perfusion media, liquids, solids or gases used in the preservation of cells, tissue, organs or bodily fluids
- A01N1/0226—Physiologically active agents, i.e. substances affecting physiological processes of cells and tissue to be preserved, e.g. anti-oxidants or nutrients
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- Health & Medical Sciences (AREA)
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- Engineering & Computer Science (AREA)
- Dentistry (AREA)
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Abstract
The present invention relates to milk cow technology of artificial insemination field more particularly to a kind of extender of freezing semen for improving milk cow conception rate；It solves milk cow and is bred using essence is frozen, the low problem of conception rate；Technical solution are as follows: include Radix Glycyrrhizae water extract, rhizoma polygonati water extract, fructus lycii water extract, vitamin B6 in the dilution；Contain isoflavones in Radix Glycyrrhizae, Siberian solomonseal rhizome polysaccharide relative molecular mass in rhizoma polygonati is all larger than 200,000, it is condensed by glucose, mannose and galacturonic acid, the polysaccharides contained in fructus lycii, these ingredients, play the role of maintaining spermatozoon activity, on the other hand, the above ingredient is dissolved in semen diluent with certain proportion, and after mixing with the frozen semen after defrosting and carrying out semen deposition, have the function of adjusting milk cow autologous hormones level in Contents in Cows, improves the conception rate of milk cow.
Description
Technical field
The present invention relates to milk cow technology of artificial insemination field more particularly to a kind of jelly essence dilutions for improving milk cow conception rate
Liquid.
Background technique
Technology of artificial insemination is the know-how that an effect is very good, highly developed in livestock reproduction, is promoting kind
Meaning is very great in terms of improvement, raising and the improvement output of live-stock product and quality.The technology of artificial insemination of milk cow is from 20th century 80
Age starts to be widely used in China, and subsequent frozen semen has gradually replaced fresh essence insemination.Recently as scale, intensive
It is universal, the output of milk of milk cow is increasing year by year, but the reproductive efficiency of milk cow is but declining.After how improving milk cow semen deposition
Conception rate and then the reproductive efficiency for improving milk cow become the hot spot of breeding scholar's research.
Summary of the invention
The present invention overcomes the deficiencies in the prior art, provide a kind of extender of freezing semen for improving milk cow conception rate.
The present invention is achieved through the following technical solutions.
A kind of extender of freezing semen improving milk cow conception rate, mentions comprising Radix Glycyrrhizae water extract, rhizoma polygonati water in the dilution
Object, fructus lycii water extract, vitamin B6.
Preferably, the Radix Glycyrrhizae water extract, rhizoma polygonati water extract, fructus lycii water extract preparation method be by Radix Glycyrrhizae, rhizoma polygonati
1-2 times of own wt of water, the filtered fluid that tanning 1-3h is obtained are separately added into fructus lycii.
Preferably, the Radix Glycyrrhizae water extract, rhizoma polygonati water extract, fructus lycii water extract, vitamin B6 weight ratio be 1:2-
4:1-2:0.5-1。
It preferably, further include prostaglandin, the concentration of the prostaglandin is 2-4mg/ml.
It preferably, further include flesh alcohol compound.
Preferably, the flesh alcohol compound is 1,3,5- phloroglucites.
Preferably, the prostaglandin is Dinoprostin.
The present invention is generated compared with the existing technology to be had the beneficial effect that.
Contain isoflavones in Radix Glycyrrhizae, the Siberian solomonseal rhizome polysaccharide relative molecular mass in rhizoma polygonati is all larger than 200,000, by glucose, sweet
Dew sugar and galacturonic acid are condensed, the polysaccharides contained in fructus lycii, these ingredients, play the role of maintaining spermatozoon activity,
On the other hand, the above ingredient is dissolved in semen diluent with certain proportion, and mixes progress with the frozen semen after defrosting
After semen deposition, has the function of adjusting milk cow autologous hormones level in Contents in Cows, improve the conception rate of milk cow.
Specific embodiment
In order to which technical problems, technical solutions and advantages to be solved are more clearly understood, in conjunction with reality
Example is applied, the present invention will be described in further detail.It should be appreciated that specific embodiment described herein is only used to explain this
Invention, is not intended to limit the present invention.Below with reference to the embodiment technical solution that the present invention will be described in detail, but protection scope not by
This limitation.
Embodiment 1
A kind of extender of freezing semen improving milk cow conception rate, raw material are Radix Glycyrrhizae water extract 10mg, rhizoma polygonati water extract 20mg, fructus lycii water
Extract 10mg, 5mg vitamin B6,5mg/ml Dinoprostin, citric acid 0.24g, lactose 5.2g, 500,000 u of penicillin, strepto-
Plain 0.2g, 1,3,5- phloroglucite 3.5g, glycerol 7.5g, fresh yolk 20ml.
It is specific the preparation method comprises the following steps:
1) 100ml, 35 DEG C of distilled water is taken to place in beaker, citric acid, the lactose, mould more than addition matched into beaker
Element, streptomysin, Radix Glycyrrhizae water extract, rhizoma polygonati water extract, fructus lycii water extract, vitamin B6,1,3,5- phloroglucites and glycerol, then
Uniform stirring 20 minutes, obtain solution.
2) pH value that citric acid regulating solution is added in above-mentioned solution is 7.0.
3) fresh egg of the fresh yolk by 55 DEG C, the inactivation treatment of 30min, in the solution in step 2 after addition sterilizing
It is yellow.Solution after mixing is placed at 4 DEG C and with 15000 revs/min of rate centrifugation 1 hour.
4) it stands, solution left standstill 30 minutes in step 3, wherein temperature was 4 DEG C, and filtering can be prepared by diluting molten later
Liquid.
Embodiment 2
A kind of extender of freezing semen improving milk cow conception rate, raw material are Radix Glycyrrhizae water extract 10mg, rhizoma polygonati water extract 30mg, fructus lycii water
Extract 20mg, 10mg vitamin B6,4.8mg/ml Dinoprostin, citric acid 0.31g, fructose 5.9g, 500,000 u of penicillin,
Streptomysin 0.3g, 1,3- cyclohexanediol 4.5g, glycerol 7.5g, fresh yolk 30ml.
Preparation method is same as Example 1.
Embodiment 3
A kind of extender of freezing semen improving milk cow conception rate, raw material are Radix Glycyrrhizae water extract 10mg, rhizoma polygonati water extract 40mg, fructus lycii water
Extract 15mg, 8mg vitamin B6,4.8mg/ml Dinoprostin, citric acid 0.21g, glucose 6.0g, 500,000 u of penicillin,
Streptomysin 0.2g, 1,3- cyclohexanediol 4.9g, glycerol 7.9g, fresh yolk 20ml.
Preparation method is same as Example 1.
The above content is combine specific preferred embodiment to the further description done of the present invention, and it cannot be said that
A specific embodiment of the invention is only limitted to this, for those of ordinary skill in the art to which the present invention belongs, is not taking off
Under the premise of from the present invention, several simple deduction or replace can also be made, all shall be regarded as belonging to the present invention by being submitted
Claims determine scope of patent protection.
Claims (7)
1. it is a kind of improve milk cow conception rate extender of freezing semen, which is characterized in that in the dilution comprising Radix Glycyrrhizae water extract,
Rhizoma polygonati water extract, fructus lycii water extract, vitamin B6.
2. a kind of extender of freezing semen for improving milk cow conception rate according to claim 1, which is characterized in that the Radix Glycyrrhizae
Water extract, rhizoma polygonati water extract, fructus lycii water extract preparation method be that Radix Glycyrrhizae, rhizoma polygonati and fructus lycii are separately added into own wt 1-2
Water again, the filtered fluid that tanning 1-3h is obtained.
3. a kind of extender of freezing semen for improving milk cow conception rate according to claim 1, which is characterized in that the Radix Glycyrrhizae
Water extract, rhizoma polygonati water extract, fructus lycii water extract, vitamin B6 weight ratio be 1:2-4:1-2:0.5-1.
4. a kind of extender of freezing semen for improving milk cow conception rate according to claim 1, which is characterized in that further include forefront
Parathyrine, the concentration of the prostaglandin are 2-4mg/ml.
5. a kind of extender of freezing semen for improving milk cow conception rate according to claim 1, which is characterized in that further include inositol
Class compound.
6. a kind of extender of freezing semen for improving milk cow conception rate according to claim 5, which is characterized in that the inositol class
Compound is 1,3,5- phloroglucites.
7. a kind of extender of freezing semen for improving milk cow conception rate according to claim 1, which is characterized in that the forefront
Parathyrine is Dinoprostin.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811368033.6A CN109287621A (en) | 2018-11-16 | 2018-11-16 | A kind of extender of freezing semen improving milk cow conception rate |
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|---|---|---|---|
| CN201811368033.6A CN109287621A (en) | 2018-11-16 | 2018-11-16 | A kind of extender of freezing semen improving milk cow conception rate |
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| CN109287621A true CN109287621A (en) | 2019-02-01 |
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| CN201811368033.6A Pending CN109287621A (en) | 2018-11-16 | 2018-11-16 | A kind of extender of freezing semen improving milk cow conception rate |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112931483A (en) * | 2021-01-29 | 2021-06-11 | 海南晨海水产有限公司 | Decapterus maruadsi sperm freezing liquid capable of keeping high survival and preparation method thereof |
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| CN107624752A (en) * | 2017-09-11 | 2018-01-26 | 吉林省农业科学院 | The preparation method of one boar freezing seminal fluid dilution |
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2018
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| CN1752207A (en) * | 2004-09-23 | 2006-03-29 | 赵有璋 | Dilution liquid of sheep frozen semen and method for producing high-quality frozen semen |
| CN1729770A (en) * | 2005-08-11 | 2006-02-08 | 西北农林科技大学 | Chinese herbal medicinal diluent for cow frozen semen |
| CN105532648A (en) * | 2016-02-16 | 2016-05-04 | 云南省畜牧兽医科学院 | Small ruminant semen freezing dilution solution |
| CN106474133A (en) * | 2016-09-21 | 2017-03-08 | 北京市农林科学院 | A kind of preparation improving milch cow conception rate and its application |
| CN107624752A (en) * | 2017-09-11 | 2018-01-26 | 吉林省农业科学院 | The preparation method of one boar freezing seminal fluid dilution |
| CN108094409A (en) * | 2018-01-12 | 2018-06-01 | 刘铮铸 | The preparation method and applications of ram freezing semen dilution |
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| SERPIL SARIÖZKAN等: "Influence of various antioxidants added to TCM-199 on post-thaw bovine sperm parameters, DNA integrity and fertilizing ability", 《CRYOBIOLOGY》 * |
| 中国科学技术情报研究所重庆分所: "《家畜繁殖技术进展》", 31 August 1979, 科学技术文献出版社重庆分社 * |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112931483A (en) * | 2021-01-29 | 2021-06-11 | 海南晨海水产有限公司 | Decapterus maruadsi sperm freezing liquid capable of keeping high survival and preparation method thereof |
| CN112931483B (en) * | 2021-01-29 | 2022-05-24 | 海南晨海水产有限公司 | Decapterus maruadsi sperm freezing liquid capable of keeping high survival and preparation method thereof |
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