CN109280627A - A method of quickly and continuously cultivating the Rhodopseudomonas photosynthetic bacteria of attached state growth - Google Patents
A method of quickly and continuously cultivating the Rhodopseudomonas photosynthetic bacteria of attached state growth Download PDFInfo
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Abstract
The invention discloses the methods of the Rhodopseudomonas photosynthetic bacteria that culture attached state is grown a kind of quickly and continuously, the water body containing phytoplankton is added first in light transmission container, and rich cellulose-containing grass or stalk are added into light transmission container, then the solid food garbage for being mixed with a water calcium dihydrogen phosphate and ironic citrate salt is laid on grass or stalk, then appropriate three water magnesium monohydrogen phosphate is added after having green periphtic algae to occur, it may occur in which that kermesinus or sanguine Rhodopseudomonas photosynthetic bacteria are largely attached on container inner wall within culture 19~25 days, the Rhodopseudomonas photosynthetic bacteria adhered on inner wall is scraped down again, it then proceedes to addition and is mixed with the food garbage of salt to be cultivated.Organic matter is rapidly converted into Rhodopseudomonas photosynthetic bacteria by this method, can promote the harmless treatment and utilization of water nutrition substance, accelerates the conversion of water nutrition substance, and the photosynthetic bacteria of formation can be directly used for aquaculture, sewage purification or new energy technology.
Description
Technical field
The present invention relates to the breeding methods of microorganisms in water, and in particular to a kind of quickly and continuously to cultivate what attached state was grown
The method of Rhodopseudomonas photosynthetic bacteria.
Background technique
Photosynthetic bacteria (Photosynthetic Bacteria) is a kind of prokaryotes with original luminous energy synthetic system
General name, non-oxygen-production photosynthesis can be carried out under anaerobic, be a kind of gramnegative bacterium.At present at sewage
It is the Rhodopseudomonas of Rhodospirillaceae that more photosynthetic bacteria type is applied in the fields such as reason, aquaculture
(Rhodopseudomonas), Prokaryota, luminous energy auxotype prokaryotes door, red light are belonged in microorganism classification
Close bacterium guiding principle, Rhodospirillales.The main feature of Rhodopseudomonas photosynthetic bacteria: being resistant to lower temperature, will not frost
It is dead；It is resistant to higher salt concentration；Containing crude protein abundant, vitamin B complex, carotenoid and ubiquinone etc..
Rhodopseudomonas photosynthetic bacteria plays a role in the various aspects such as aquaculture, sewage treatment, new energy research,
It is main: (1) to purify water；(2) organic matter oxygen consumption is reduced；(3) bait of zooplankter；(4) additive capable of using as feed；(5) it produces
Raw hydrogen etc..
The conventional method of culture Rhodopseudomonas photosynthetic bacteria is the culture medium using human configuration at present, in addition there is phase
Several large-scaled culture methods that research has discovered that Rhodopseudomonas photosynthetic bacteria are closed, culture raw material can be cooperation
Feed is also possible to food garbage, is also possible to the culture medium of a variety of chemical substance configurations.Red vacation is mainly utilized in these methods
Zygosaccharomyces photosynthetic bacteria can degrade, using organic matter the characteristics of, but directly utilize mixed feed or food garbage culture
When need organic matter to rot under appropriate Oxygen Condition, so that required incubation time is longer (at least at 35 days or more), and sharp
Though incubation time is short but need a variety of chemical raw materials and to the configuration of each substance ratio when configuring culture medium with a variety of chemical substances
Example has compared with strict requirements.Also there is the research for immobilizing Rhodopseudomonas photosynthetic bacteria, mainly utilize
Solid material is made into molecule, and allows red pseudomonas apposition growth on these molecules, certain hydrodynamic having
Under the conditions of these molecules for being attached to red pseudomonas can be suspended in water body, reach and promote the photosynthetic bacterium growth
Purpose；But the culture medium selected in these researchs is usually the culture medium of general a variety of chemical raw materials configuration.
Summary of the invention
When being needed the purpose of the present invention is to the large-scaled culture method of existing Rhodopseudomonas photosynthetic bacteria
Between technological deficiency that is long or needing a variety of chemical raw materials, provide it is a kind of quickly and the red pseudomonas of continuous culture attached state growth
The method for belonging to photosynthetic bacteria is more advantageous in conjunction with the large-scaled culture method of above-mentioned existing Rhodopseudomonas photosynthetic bacteria
The stabilization of Rhodopseudomonas photosynthetic bacteria, quick, pilot scale culture.
In order to achieve the above technical purposes, one kind that the present invention designs quickly and continuously cultivates the red false single of attached state growth
The method of born of the same parents Pseudomonas photosynthetic bacteria, which is characterized in that it including the following steps:
S1, light transmission container is placed on to glasshouse or outdoor natural light according in environment, the environment temperature on daytime and night
Variation range is added with the water body of phytoplankton into light transmission container at 15~45 DEG C, and addition is rich in fibre into light transmission container
The grass or stalk of element are tieed up, guarantees that grass or stalk swim in water body upper layer；
S2, the solid food garbage for being mixed with saline admixture is added on grass or stalk, addition is rich in step S1
The grass of cellulose or the amount of stalk are enough to allow solid food garbage not submerged, and solid food garbage is submerged in water just
In；
S3, culture 3~5 days, water body green thickens or even becomes brown-green；
The region that culture 6~10 days, container inner wall top and grass are close to grows green periphtic algae；
Culture 11~14 days, container inner wall top bottle green periphtic algae area expands, and bottle green attachment is occurring
Three water magnesium monohydrogen phosphate (MgHPO are added when algal grown4·3H2O), additive amount is 4~15g；
S4, it cultivates 15~18 days, the attachment of kermesinus occurs in container inner wall upper green periphtic algae, and dark green
The periphtic algae of color gradually becomes crineous；
Culture 19~25 days, the crineous attachment on container inner wall become kermesinus or sanguine red false unit cell comprehensively
Pseudomonas photosynthetic bacteria, and the region that container inner wall top is close to grass may be changed into bottle green or continue to keep dark red
Color；
The Rhodopseudomonas photosynthetic bacteria of apposition growth cloth in the case where water in container is not discharged in S5, step S4
It scrapes down；Then continue the food garbage that addition is mixed with salt on grass or stalk in a reservoir, the amount of addition is no more than
The preceding amount once added, and do not have to add three water magnesium monohydrogen phosphates in the follow-up process, thus realize continuously repeatedly culture, until
Grass or stalk therein have rotted and food garbage cannot be supported in the degradation of the water surface.
Further, the step S1 is carried out in late April~early November in warm season.
Further, in the step S2, the adding proportion of the solid food garbage and water body is solid state food and drink rubbish
Rubbish: water body=15~65g:1L, and ulking thickness of the food garbage on grass or stalk is no more than 3cm, solid food garbage quilt
Water floods completely.
Further, in the step S2, saline admixture includes a water calcium dihydrogen phosphate [Ca (H2PO4)2·H2O] and
Ironic citrate, wherein the quality adding proportion of the water calcium dihydrogen phosphate be solid food garbage quality 0.25%~
0.75%, the quality adding proportion of ironic citrate is 0.1 ‰~the 0.25 ‰ of solid food garbage quality.
Further, in the step S2, the solid food garbage is remained after removing the substance of wherein non-food class
Remaining food rubbish, including in rice, wheaten food, fish, meat, egg, veterinary antibiotics, salt, the condiments cooked, bone, eggshell etc.
It is several or whole, and based on the starchy materials such as rice, wheaten food, it is ensured that liquid water is few as far as possible in food garbage, and spreads out
It is spare after air-drying 1 hour.
Further, in the step S1, the light transmission container material is Plain transparent glass or transparent organic glass
Glass or white light-passing plastic or colourless transparent plastic.
Further, in the step S1, the grass or stalk are that straw or greening lawn be careless or wheat stalk,
It is able to maintain solid food garbage in the water surface without sinking under water after accumulation certain thickness.
Further, in the step S4, the maximum gauge of the Rhodopseudomonas photosynthetic bacteria of apposition growth can reach
About 1~2mm supplements tap water, supplement evaporation water consumption at any time during culture.
Further, in the step S1, in the poisons in freshwater containing phytoplankton initially added total nitrogen concentration 2~
30mg/L, chlorophyll-a concentration is in 30~2700 μ g/L.
In technical solution of the present invention, the addition of salt in solid food garbage, transparent vessel, solid food garbage and floating
It is to realize invention that the adding proportion of plant water body abundant, temperature range, solid food garbage, which are swum, in 5 conditions such as water surface degradations
The key of purpose.The purpose for adding hay in a reservoir is to allow food garbage and be located at for support in water with the grass floated
Face is easy to allow food garbage to touch the oxygen in air and the degradation that can rapidly rot.
A water calcium dihydrogen phosphate and ironic citrate are added in solid food garbage, main function is can to promote food and drink rubbish
The degradation of rubbish, and can supplement the metal cations such as calcium, iron is conducive to meet subsequent Rhodopseudomonas photosynthetic bacteria and largely adhere to
The demand of growth.
The case where solid food garbage will appear stink and vinasse taste and be deposited in degradation process.When solid food garbage and
When the adding proportion of water is lower than 15g:1L, food garbage generates obvious vinasse taste after water surface degradation, and (i.e. degradation is produced
Ethyl alcohol) hold time it is longer, and food garbage rot after the nutrient concentrations such as Water, phosphorus that water body can be supported to occur is green
Color periphtic algae, but green periphtic algae is not easy to be changed into red particle-bound bacteria；When the addition ratio of solid food garbage and water
When example is greater than 65g:1L or food garbage is when the ulking thickness on grass is no more than 3cm, and food garbage is after water surface degradation
Very heavy stink (producing ammonia mainly due to degradation) can be generated, water body entirety anoxic, the corruption of solid food garbage are be easy to cause
Rotten degradation time can be grown partially, and subsequent water body grows time of green periphtic algae and can postpone, and it is attached to occur rhodobacterium on the whole
Time can postpone and influence cultivate Rhodopseudomonas photosynthetic bacteria time efficiency.
Addition is insoluble in three water magnesium monohydrogen phosphate (MgHPO of water when there is the growth of bottle green periphtic algae4·
3H2O), because it is under the conditions of anaerobism or the slant acidity of hypoxemia can be released with slow reaction a small amount of magnesium ion and phosphate radical from
Son can provide sufficient magnesium elements for the Rhodopseudomonas rich in bacteriochlorophyll, and not influence other ions in water body and contain
Amount.Three water magnesium monohydrogen phosphates can be changed to soluble epsom salt, only epsom salt is soluble, great Liang Tian
Added-time can change the salinity of water body, be dispersed in water body and be not readily observed after dissolution, and three water magnesium monohydrogen phosphates can be deposited in
The bottom picks up some salt powders from glass jar bottom after rhodobacterium is grown on inner wall, can obviously observe red thin
Bacterium apposition growth is in these salts.
Glasshouse or outdoor natural light according to the variation of ambient temperature range at daytime in environment and night at 15~45 DEG C,
Precisely first warm season of autumn Mo in the Summer Solstice, temperature is higher, illumination is abundant, it is ensured that suitable temperature, illumination condition.If
When being temperature relatively low winter or spring, the degradation of solid state food garbage can be slack-off, although green algae can be grown, goes out
Existing rhodobacterium attachment needs the longer time.
In technical solution of the present invention, in the step S2, total nitrogen in the phytoplankton of addition poisons in freshwater abundant
Concentration readily satisfies this model in 30~2700 μ g/L, general natural fresh water water body in 2~30mg/L, chlorophyll-a concentration
It encloses；And the high concentration phytoplankton water body generated after organic matter rots can be selected, wherein total nitrogen concentration may be up to 30mg/L, float
Trip plant concentration may be up to 2700 μ g/L.Selecting the purpose in water body containing a certain amount of phytoplankton is by plant of swimming in water body
The photosynthesis of object increases the oxygen content in water body, and provides diversity Initial microorganisms group abundant, thus favorably
In the rotten degradation of Initial stage of culture food garbage.
Secondly, the purposes the invention further relates to the photosynthetic bacteria of method generation in aquaculture.The present invention obtains
Photosynthetic bacteria processing aquaculture residual bait or fowl poultry kind excreta, can be used for the adjusting of water quality in breeding water, plant of swimming
The regulation of object structure of community or as feed addictive, and good natural bait can be provided for zooplankter, it also can conduct
The high-quality bait of chironomus larvas is used for mass propgation chironomus larvas.
It, can be in addition, the invention further relates to purposes of the photosynthetic bacteria in the dirty water decontamination handles that the method is formed
Accelerate the degradation of organic matter in sewage.
Again, the purposes the invention further relates to the photosynthetic bacteria of method formation in terms of new energy, can be used to
Efficient hydrogen making.
The utility model has the advantages that
The method of the Rhodopseudomonas photosynthetic bacteria that culture attached state is grown a kind of quickly and continuously provided by the invention,
Compared with prior art, it has the advantages that following several respects:
1, raw material is easy to obtain, and raw material sources are abundant, at low cost；
2, the present invention provides simple, practical method for the pilot scale culture of photosynthetic bacteria, and incubation time is than previous
Method is shorter, more efficient.
3, the thick pulpous state photosynthetic bacteria that attachment state is grown on the container inner wall that the present invention obtains, with state growth of swimming
Photosynthetic bacteria compare, the concentrated cost of photosynthetic bacteria can be substantially reduced, and transport and save and all save space, it is extremely convenient.
4, cultural method of the invention promotes rotting for organic matter without the measures such as agitation or aeration, greatly saves
Economic cost.
5, the high concentration photosynthetic bacteria that method of the invention is formed can be directly used for processing breeding wastewater or livestock and poultry excretion
Object also can be used as the good natural bait of zooplankter, also can be directly used for the dirty water decontamination handles, efficient hydrogen making.
Detailed description of the invention
Attached drawing 1 is the photo of the Rhodopseudomonas photosynthetic bacteria for the attached state growth cultivated in the embodiment of the present invention 1；
Attached drawing 2 is the photo of the Rhodopseudomonas photosynthetic bacteria for the attached state growth cultivated in the embodiment of the present invention 2；
Attached drawing 3 is the photo of the Rhodopseudomonas photosynthetic bacteria for the attached state growth cultivated in the embodiment of the present invention 3；
Attached drawing 4 is the photo of the Rhodopseudomonas photosynthetic bacteria for the attached state growth cultivated in the embodiment of the present invention 4；
Attached drawing 5 is the photo of the Rhodopseudomonas photosynthetic bacteria for the attached state growth cultivated in the embodiment of the present invention 5.
Specific embodiment
The following describes the present invention in detail with reference to examples.Following embodiment will be helpful to those skilled in the art
The present invention is further understood, but the invention is not limited in any way.It should be pointed out that those skilled in the art
For, without departing from the inventive concept of the premise, it can also make certain adjustments and improvements.These belong to guarantor of the invention
Protect range.
Embodiment 1
By the end of April, specification 60cm × 40cm × 40cm flint glass cylinder is placed in glasshouse, into glass jar
Addition utilizes the water body 80L containing phytoplankton of originally Aquaponic, and total nitrogen concentration is 2.0mg/L, chlorophyll-a concentration in the water body
For 30 μ g/L, and the green grass cut off on landscape meadow is added into glass jar, green grass laying uniform as far as possible, green grass in glass jar
Addition thickness about 5cm, green grass swims in water body upper layer.
Food garbage is obtained from restaurant, after removing non-food substance therein, water is drained and is spread out air-dried 1 small
When.1200g solid food garbage is weighed, mono- water calcium dihydrogen phosphate [Ca (H of 3g is weighed2PO4)2·H2O], 0.12g ironic citrate,
2 kinds of salts are mixed into food garbage tiling after mixing evenly and are added to green grass surface, and the piling height of part is no more than
3cm, and food garbage floods just and is advisable in water, laid eggs on food garbage with mosquito proof generate the mosquitoes young such as wriggler and
Reduce the utilization rate of food garbage.
Culture 3 days, water body green thickens；Culture 6 days, water body color becomes brown-green, and container inner wall by bottle green
The region that top and grass are located next to grows green periphtic algae；Culture 11 days, the bottle green periphtic algae area on container inner wall
Expand；12nd day, tri- water magnesium monohydrogen phosphate (MgHPO of 4g was added into the water body below grass4·3H2O)。
After culture 15 days, water body color is still green middle band brown, and container inner wall top and grass are located next to the green in region
Occurs the attachment of kermesinus in periphtic algae, and bottle-green periphtic algae gradually becomes crineous；After culture 19 days, hold
Crineous attachment on device inner wall becomes kermesinus comprehensively, and the high throughput of microorganism is carried out using Illumina MiSeq platform
Analysis detection shows that the kermesinus particle-bound bacteria is that Rhodopseudomonas Rhodopseudomonas (Rhodopseudomonas) is photosynthetic
Bacterium, and dominant species are excrement red pseudomonas (Rhodopseudomonas faecalis), that is, form the red of apposition growth
Pseudomonas photosynthetic bacteria.With its continued growth, the maximum gauge energy of the Rhodopseudomonas photosynthetic bacteria of apposition growth
Reach about 1mm.
The Rhodopseudomonas photosynthetic bacteria of apposition growth can in the case where water in container is not discharged with cloth scraping or
Person's other methods scrape down, and then continue to add same amount of food garbage on grass in a reservoir, wherein being mixed with identical
A water calcium dihydrogen phosphate and ironic citrate for ratio, the amount of addition are advisable with being no more than the preceding amount once added, and are not had to rear
Three water magnesium monohydrogen phosphates are added during continuous.Since the Rhodopseudomonas photosynthetic bacteria and others that also have more in water body can
It degrades the bacterium of organic matter, the time of follow-up cultivation with regard to shorter, can allow on inner wall and cover with the red false unit cell of attached state again for 5 days
Pseudomonas photosynthetic bacteria.It may then continue with and scrape down the red pseudomonas of attachment, and can add again and be mixed with salt
Food garbage culture.To realize continuous repeatedly culture, until grass therein has rotted and food garbage cannot have been supported to exist
Water surface degradation.
By the end of April-this period by the end of May in, the room temperature variation range at daytime and night is 15~42 in glasshouse
℃.The photo of the Rhodopseudomonas photosynthetic bacteria of the attached state growth of the present embodiment culture is as shown in Fig. 1.
Embodiment 2
By the end of April, 60cm × 35cm × 40cm flint glass cylinder is placed in glasshouse, benefit is added into glass jar
With the water body 70L containing phytoplankton of originally Aquaponic, total nitrogen concentration is 30mg/L in the water body, and chlorophyll-a concentration is 2700 μ
G/L, wherein Chlorophyta Scenedesmus phytoplankton rich in.And the blueness cut off on landscape meadow is added into glass jar
Grass, the addition thickness about 7cm of green grass, green grass swim in water body upper layer in glass jar.
Food garbage is obtained from restaurant, after removing non-food substance therein, water is drained and is spread out air-dried 1 small
When.4550g solid food garbage is weighed, mono- water calcium dihydrogen phosphate of 22.75g and 1.14g ironic citrate is weighed, 2 kinds of salts is mixed
It closes and is tiled after mixing evenly in food garbage to green grass surface, and the piling height of part is no more than 3cm, it is proper with food garbage
Flood well is advisable in water, is laid eggs on food garbage with mosquito proof and generates the mosquitoes young such as wriggler and reduce the benefit of food garbage
With rate.
Culture 3 days, water body becomes brown-green；Culture 8 days, water body color become burgundy, and container inner wall top with
The region that grass is located next to grows green periphtic algae；Culture 12 days, bottle green periphtic algae area is expanded to about on container inner wall
The half of whole inner wall areas；13rd day, tri- water magnesium monohydrogen phosphate of 15g is added in the water body below grass.
After culture 18 days, there is kermesinus in the green periphtic algae in container inner wall top and the careless region being located next to
Attachment, and bottle-green periphtic algae gradually becomes crineous, also becomes burgundy in water body；After culture 22 days, container
Crineous attachment on inner wall becomes blood red comprehensively, and the high throughput point of microorganism is carried out using Illumina MiSeq platform
Analysis detection shows that the blood red particle-bound bacteria is Rhodopseudomonas (Rhodopseudomonas) photosynthetic bacteria, and dominant species
It is excrement red pseudomonas (Rhodopseudomonas faecalis), that is, the Rhodopseudomonas for foring apposition growth is photosynthetic
Bacterium.With its continued growth, the maximum gauge of the Rhodopseudomonas photosynthetic bacteria of apposition growth can reach about 2mm.
The Rhodopseudomonas photosynthetic bacteria of apposition growth can in the case where water in container is not discharged with cloth scraping or
Person's other methods scrape down, and then continue the food garbage for adding one third amount on grass in a reservoir, wherein being mixed with
A water calcium dihydrogen phosphate and ironic citrate for same ratio, and do not add three water magnesium monohydrogen phosphates in the follow-up process.Due to water body
In also have the bacterium of more Rhodopseudomonas photosynthetic bacteria and other degradable organic matters, the time of follow-up cultivation is just
It is shorter, it can allow within 8 days on inner wall and cover with the Rhodopseudomonas photosynthetic bacteria of attached state again.It may then continue with attachment
Red pseudomonas scrapes down, and can add the food garbage culture for being mixed with salt again.It is continuous multiple to realize
Culture, until grass therein has rotted and food garbage cannot have been supported to degrade in the water surface.
In this period at the beginning of -6 months by the end of April, the room temperature variation range at daytime and night is 15~42 in glasshouse
℃.The photo of the Rhodopseudomonas photosynthetic bacteria of the attached state growth of the present embodiment culture is as shown in Fig. 2.
Embodiment 3
By the end of April, 60cm × 35cm × 40cm flint glass cylinder is placed in glasshouse, benefit is added into glass jar
With the water body 70L containing phytoplankton of originally Aquaponic, total nitrogen concentration is 20.3mg/L in the water body, and chlorophyll-a concentration is
1260 μ g/L, wherein Chlorophyta Scenedesmus phytoplankton rich in.And it adds into glass jar and is cut off on landscape meadow
Green grass, green grass spreads uniformly as far as possible, and the addition thickness about 6cm of green grass, green grass swim in water body upper layer in glass jar.
Food garbage is obtained from restaurant, after removing non-food substance therein, water is drained and is spread out air-dried 1 small
When.3600g solid food garbage is weighed, a water calcium dihydrogen phosphate, ironic citrate 27g, 0.54g is weighed respectively, salt is mixed
Into food garbage, green grass surface is arrived in tiling after mixing evenly, and the piling height of part is no more than 3cm, and food garbage is lucky
Flood is advisable in water, is laid eggs on food garbage with mosquito proof and generates the mosquitoes young such as wriggler and reduce the utilization of food garbage
Rate.
It cultivates 3 days, band point brown in water body green；Culture 9 days, water body color becomes brown, and container by bottle green
The region that upper inside wall and grass are located next to grows green periphtic algae；Culture 12 days, the bottle green periphtic algae on container inner wall
Area is expanded to the half of about whole inner wall area；14th day, tri- water magnesium monohydrogen phosphate of 12g is added in the water body below grass
(MgHPO4·3H2O)。
After culture 18 days, there is kermesinus in the green periphtic algae in container inner wall top and the careless region being located next to
Attachment, and bottle-green periphtic algae gradually becomes crineous；After culture 22 days, the crineous attachment on container inner wall is complete
Face becomes kermesinus, shows that the kermesinus is adhered to using the high throughput analysis detection that Illumina MiSeq platform carries out microorganism
Bacterium is Rhodopseudomonas (Rhodopseudomonas) photosynthetic bacteria, and dominant species are excrement red pseudomonas
(Rhodopseudomonas faecalis) forms the Rhodopseudomonas photosynthetic bacteria of apposition growth.With its continuation
Growth, the maximum gauge of the Rhodopseudomonas photosynthetic bacteria of apposition growth can reach about 2mm.
The Rhodopseudomonas photosynthetic bacteria of apposition growth can in the case where water in container is not discharged with cloth scraping or
Person's other methods scrape down, and then continue the food garbage for adding one third amount on grass in a reservoir, wherein being mixed with
A water calcium dihydrogen phosphate and ironic citrate for same ratio, and do not add three water magnesium monohydrogen phosphates in the follow-up process.Due to water body
In also have the bacterium of more Rhodopseudomonas photosynthetic bacteria and other degradable organic matters, the time of follow-up cultivation is just
It is shorter, it can allow within 8 days on inner wall and cover with the Rhodopseudomonas photosynthetic bacteria of attached state again.It may then continue with attachment
Red pseudomonas scrapes down, and can add the food garbage culture for being mixed with salt again.It is continuous multiple to realize
Culture, until grass therein has rotted and food garbage cannot have been supported to degrade in the water surface.
By the end of April-this period by the end of May in, the room temperature variation range at daytime and night is 15~42 in glasshouse
℃.The photo of the Rhodopseudomonas photosynthetic bacteria of the attached state growth of the present embodiment culture is as shown in Fig. 3.
Embodiment 4
By the end of July, 10L flint glass wide-mouth bottle cylinder is placed in glasshouse, into glass jar, addition is using originally
The water body 10L containing phytoplankton of Aquaponic, total nitrogen concentration is 24.6mg/L in the water body, and chlorophyll-a concentration is 1178 μ g/L,
Wherein Chlorophyta Scenedesmus phytoplankton rich in.And the green grass cut off on landscape meadow is added into glass jar, it is green
Grass spreads uniformly as far as possible, and the addition thickness about 5cm of green grass, green grass swim in water body upper layer in glass jar.
Food garbage is obtained from restaurant, after removing non-food substance therein, water is drained and is spread out air-dried 1 small
When.500g solid food garbage is weighed, mono- water calcium dihydrogen phosphate of 2g, 0.075g ironic citrate is weighed, salt is mixed into food and drink
Green grass surface is arrived in tiling after mixing evenly in rubbish, and the piling height of part is no more than 3cm, and food garbage is submerged in just
It is advisable in water, is laid eggs on food garbage with mosquito proof and generate the mosquitoes young such as wriggler and reduce the utilization rate of food garbage.
Culture 3 days, water body green become brown-green；Culture 9 days, water body color become brown, and container inner wall top with
The region that grass is located next to grows green periphtic algae；Culture 12 days, the bottle green periphtic algae area on container inner wall is expanded to
The about half of entire area；14th day, tri- water magnesium monohydrogen phosphate of 4g is added in the water body below grass.
After culture 18 days, there is the attached of kermesinus in the green periphtic algae in region with grass in container inner wall top
Object, and bottle-green periphtic algae gradually becomes crineous；After culture 22 days, the crineous attachment on container inner wall is comprehensive
Become blood red, it is thin to show that the blood red is adhered to using the high throughput analysis detection that Illumina MiSeq platform carries out microorganism
Bacterium is Rhodopseudomonas (Rhodopseudomonas) photosynthetic bacteria, and dominant species are excrement red pseudomonas
(Rhodopseudomonas faecalis) forms the Rhodopseudomonas photosynthetic bacteria of apposition growth.With its continuation
Growth, the maximum gauge of the Rhodopseudomonas photosynthetic bacteria of apposition growth can reach about 2mm.
The Rhodopseudomonas photosynthetic bacteria of apposition growth can in the case where water in container is not discharged with cloth scraping or
Person's other methods scrape down, and then continue the food garbage for adding one third amount on grass in a reservoir, wherein being mixed with
A water calcium dihydrogen phosphate and ironic citrate for same ratio, and do not add three water magnesium monohydrogen phosphates in the follow-up process.Due to water body
In also have the bacterium of more Rhodopseudomonas photosynthetic bacteria and other degradable organic matters, the time of follow-up cultivation is just
It is shorter, it can allow within 8 days on inner wall and cover with the Rhodopseudomonas photosynthetic bacteria of attached state again.It may then continue with attachment
Red pseudomonas scrapes down, and can add the food garbage culture for being mixed with salt again.It is continuous multiple to realize
Culture, until grass therein has rotted and food garbage cannot have been supported to degrade in the water surface.
By the end of July-September at the beginning of this period in, the room temperature variation range at daytime and night is 20~45 in glasshouse
℃.The photo of the Rhodopseudomonas photosynthetic bacteria of the attached state growth of the present embodiment culture is as shown in Fig. 4.
Embodiment 5
By the end of September, 60cm × 35cm × 40cm flint glass cylinder is placed in glasshouse, benefit is added into glass jar
With the water body 40L containing phytoplankton of originally Aquaponic, total nitrogen concentration is 15.8mg/L, chlorophyll-a concentration 565 in the water body
μ g/L, wherein Chlorophyta Scenedesmus rich in and Ankistrodesmus phytoplankton.And it is added on landscape meadow into glass jar
The green grass cut off, green grass spread uniformly as far as possible, and the addition thickness about 4cm of green grass, green grass swim in water body upper layer in glass jar.
Food garbage is obtained from restaurant, after removing non-food substance therein, water is drained and is spread out air-dried 1 small
When.2000g solid food garbage is weighed, mono- water calcium dihydrogen phosphate of 6g, 0.3g ironic citrate is weighed, salt is mixed into food and drink rubbish
Green grass surface is arrived in tiling after mixing evenly in rubbish, and the piling height of part is no more than 3cm, and food garbage is submerged in water just
In be advisable, laid eggs on food garbage with mosquito proof and generate the mosquitoes young such as wriggler and reduce the utilization rate of food garbage.
It cultivates 3 days, band light brown in water body green；Culture 9 days, water body color becomes brown, and container inner wall top
The region being located next to grass grows green periphtic algae；Culture 12 days, the bottle green periphtic algae area on container inner wall expand
To the half of about entire area；14th day, tri- water magnesium monohydrogen phosphate of 6g is added in the water body below grass.
After culture 18 days, there is kermesinus in the green periphtic algae in container inner wall top and the careless region being located next to
Attachment, and bottle-green periphtic algae gradually becomes crineous；After culture 20 days, the crineous attachment on container inner wall is complete
Face becomes kermesinus, shows that the kermesinus is adhered to using the high throughput analysis detection that Illumina MiSeq platform carries out microorganism
Bacterium is Rhodopseudomonas (Rhodopseudomonas) photosynthetic bacteria, and dominant species are excrement red pseudomonas
(Rhodopseudomonas faecalis) forms the Rhodopseudomonas photosynthetic bacteria of apposition growth.With its continuation
Growth, the maximum gauge of the Rhodopseudomonas photosynthetic bacteria of apposition growth can reach about 2mm.
The Rhodopseudomonas photosynthetic bacteria of apposition growth can in the case where water in container is not discharged with cloth scraping or
Person's other methods scrape down, and then continue the food garbage for adding one third amount on grass in a reservoir, wherein being mixed with
A water calcium dihydrogen phosphate and ironic citrate for same ratio, and do not add three water magnesium monohydrogen phosphates in the follow-up process.Due to water body
In also have the bacterium of more Rhodopseudomonas photosynthetic bacteria and other degradable organic matters, the time of follow-up cultivation is just
It is shorter, it can allow within 8 days on inner wall and cover with the Rhodopseudomonas photosynthetic bacteria of attached state again.It may then continue with attachment
Red pseudomonas scrapes down, and can add the food garbage culture for being mixed with salt again.It is continuous multiple to realize
Culture, until grass therein has rotted and food garbage cannot have been supported to degrade in the water surface.
By the end of September-this period by the end of October in, the room temperature variation range at daytime and night is 20~45 in glasshouse
℃.The photo of the Rhodopseudomonas photosynthetic bacteria of the attached state growth of the present embodiment culture is as shown in Fig. 5.
Structure depicted in this specification institute accompanying drawings of the embodiment of the present invention, ratio, size, quantity etc., only to cooperate specification
Revealed content is not intended to limit the invention enforceable restriction item so that those skilled in the art understands and reads
Part, therefore do not have technical essential meaning, any modification, the change of proportionate relationship or the adjustment of size are not influencing this hair
Under bright the effect of can be generated and the purpose that can reach, it should all still fall in disclosed technology contents and obtain and can cover
In range.
Claims (9)
1. the method for the Rhodopseudomonas photosynthetic bacteria that culture attached state is grown a kind of quickly and continuously, which is characterized in that it
Including the following steps:
S1, light transmission container is placed on to glasshouse or outdoor natural light according in environment, the room temperature variation range at daytime and night
At 15~45 DEG C, it is added with the water body of phytoplankton into light transmission container, and adds rich cellulose-containing grass into light transmission container
Or stalk, guarantee that grass or stalk swim in water body upper layer；
S2, the solid food garbage for being mixed with saline admixture is added on grass or stalk, addition is rich in fiber in step S1
The grass of element or the amount of stalk are enough to allow solid food garbage not submerged, and solid food garbage floods in water just；
S3, culture 3~5 days, water body green thickens or even becomes brown-green；
The region that culture 6~10 days, container inner wall top and grass are close to grows green periphtic algae；
Culture 11~14 days, container inner wall top bottle green periphtic algae area expands, and bottle green periphtic algae is occurring
Three water magnesium monohydrogen phosphate (MgHPO are added when growth4·3H2O), additive amount is 4~15g；
S4, it cultivates 15~18 days, the attachment of kermesinus occurs in container inner wall upper green periphtic algae, and bottle-green
Periphtic algae gradually becomes crineous；
Culture 19~25 days, the crineous attachment on container inner wall becomes kermesinus or sanguine Rhodopseudomonas comprehensively
Photosynthetic bacteria, and the region that container inner wall top and grass are close to may be changed into bottle green or continue to keep kermesinus；
The Rhodopseudomonas photosynthetic bacteria of apposition growth is scraped in the case where water in container is not discharged with cloth in S5, step S4
Get off；Then continue the food garbage that addition is mixed with salt on grass or stalk in a reservoir, the amount of addition is no more than previous
The amount of secondary addition, and do not have to add three water magnesium monohydrogen phosphates in the follow-up process, so that continuously repeatedly culture is realized, until wherein
Grass or stalk rotted and food garbage cannot be supported in the degradation of the water surface.
2. a kind of side of Rhodopseudomonas photosynthetic bacteria for quickly and continuously cultivating attached state growth as described in claim 1
Method, it is characterised in that: the step S1 is carried out in late April~early November in warm season.
3. a kind of side of Rhodopseudomonas photosynthetic bacteria for quickly and continuously cultivating attached state growth as described in claim 1
Method, it is characterised in that: in the step S2, the adding proportion of the solid food garbage and water body is solid state food garbage:
Water body=15~65g:1L, and ulking thickness of the food garbage on grass or stalk is no more than 3cm, solid food garbage is complete by water
Total flooding.
4. a kind of side of Rhodopseudomonas photosynthetic bacteria for quickly and continuously cultivating attached state growth as described in claim 1
Method, it is characterised in that: in the step S2, saline admixture includes a water calcium dihydrogen phosphate [Ca (H2PO4)2·H2O] and lemon
Sour iron, wherein the quality adding proportion of the water calcium dihydrogen phosphate is the 0.25%~0.75% of solid food garbage quality,
The quality adding proportion of ironic citrate is 0.1 ‰~the 0.25 ‰ of solid food garbage quality.
5. a kind of side of Rhodopseudomonas photosynthetic bacteria for quickly and continuously cultivating attached state growth as described in claim 1
Method, it is characterised in that: in the step S2, the solid food garbage is remaining after removing the substance of wherein non-food class
Food rubbish, including several in rice, wheaten food, fish, meat, egg, veterinary antibiotics, salt, the condiments cooked, bone, eggshell
Or all, and based on the starchy materials such as rice, wheaten food, it is ensured that liquid water is few as far as possible in food garbage, and it is small to spread air-dried 1 out
Shi Houbei is used.
6. a kind of side of Rhodopseudomonas photosynthetic bacteria for quickly and continuously cultivating attached state growth as described in claim 1
Method, it is characterised in that: in the step S1, the light transmission container material be Plain transparent glass or transparent organic glass or
White light-passing plastic or colourless transparent plastic.
7. a kind of side of Rhodopseudomonas photosynthetic bacteria for quickly and continuously cultivating attached state growth as described in claim 1
Method, it is characterised in that: in the step S1, the grass or stalk refer to straw or greening lawn grass or wheat stalk,
It is able to maintain solid food garbage in the water surface without sinking under water after accumulation certain thickness.
8. a kind of side of Rhodopseudomonas photosynthetic bacteria for quickly and continuously cultivating attached state growth as described in claim 1
Method, it is characterised in that: in the step S4, the maximum gauge of the Rhodopseudomonas photosynthetic bacteria of apposition growth can reach about 1
~2mm supplements tap water, supplement evaporation water consumption at any time during culture.
9. a kind of side of Rhodopseudomonas photosynthetic bacteria for quickly and continuously cultivating attached state growth as described in claim 1
Method, it is characterised in that: in the step S1, total nitrogen concentration is in 2~30mg/ in the poisons in freshwater containing phytoplankton that initially adds
L, chlorophyll-a concentration is in 30~2700 μ g/L.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105754903A (en) * | 2016-04-15 | 2016-07-13 | 中国水产科学研究院渔业机械仪器研究所 | Method for cultivating photosynthetic bacteria in Rhodopseudomonas on large scale |
| CN106282056A (en) * | 2016-08-12 | 2017-01-04 | 中国水产科学研究院渔业机械仪器研究所 | A kind of method utilizing food garbage to cultivate Rhodopseudomonas photosynthetic bacteria |
| CN107686815A (en) * | 2017-10-17 | 2018-02-13 | 中国水产科学研究院渔业机械仪器研究所 | A kind of method that chlorella is cultivated using food garbage |
| CN107858313A (en) * | 2017-12-12 | 2018-03-30 | 中国水产科学研究院渔业机械仪器研究所 | Culture medium and cultural method for Rhodopseudomonas photosynthetic bacteria apposition growth |
-
2018
- 2018-06-29 CN CN201810694582.6A patent/CN109280627B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105754903A (en) * | 2016-04-15 | 2016-07-13 | 中国水产科学研究院渔业机械仪器研究所 | Method for cultivating photosynthetic bacteria in Rhodopseudomonas on large scale |
| CN106282056A (en) * | 2016-08-12 | 2017-01-04 | 中国水产科学研究院渔业机械仪器研究所 | A kind of method utilizing food garbage to cultivate Rhodopseudomonas photosynthetic bacteria |
| CN107686815A (en) * | 2017-10-17 | 2018-02-13 | 中国水产科学研究院渔业机械仪器研究所 | A kind of method that chlorella is cultivated using food garbage |
| CN107858313A (en) * | 2017-12-12 | 2018-03-30 | 中国水产科学研究院渔业机械仪器研究所 | Culture medium and cultural method for Rhodopseudomonas photosynthetic bacteria apposition growth |
Non-Patent Citations (2)
| Title |
|---|
| TAKAAKI FINN ET AL.: "Utilization of Alcohols by Rhodopseudomonas sp. No.7 Isolated from n-Propanol-Enrichment Cultures", 《AGRICULTURAL AND BIOLOGICAL CHEMISTRY》 * |
| 陈声明等: "一种光合细菌— 红假单胞菌分离物培养条件的研究", 《浙江农业大学学报》 * |
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