CN109266532A - It is used to prepare washing and the separation vessel of adipose tissue - Google Patents

Abstract

The present invention relates to container, device and the external members of the adipose tissue for can be used in injection from the fat material preparation pumped out.Specifically, the washing and separation vessel that are used to prepare adipose tissue have the washing chamber of the fat material for washing suction, the container has entrance and exit, the chamber is left for making fat material pass through outlet by the fluid components that entrance enters washing chamber and makes the material, mixing component including at least one emulsion for being used to mechanically form fluid components in the washing chamber, each mixing component include elastomeric element and at least one sphere for being fixed on elastomeric element.Since elastomeric element and sphere are connected, it slight concussion and rocks and can also cause the movement of bead by a relatively large margin in container, and the overturning of spring causes indifference eddy motion, to more effectively emulsion be promoted to be formed, thus can streamline operation, improve working efficiency.

Description

It is used to prepare washing and the separation vessel of adipose tissue

Technical field

The present invention relates to container, the devices of the adipose tissue for can be used in injection from the fat material preparation pumped out And external member.

Background technique

Early in the seventies of eighties of last century, autologous fat transplantation become plastic surgery circle confirmation method after, just gradually by It is applied to the every field of beauty and shaping.Autologous fat transplantation needs handle the fat of sucking, it is important that must be first The fatty oil component generated in red blood cell and broken fat cell in the sucking fat of discharge.This is because red blood cell point Normal tissue is destroyed at branch after solution, to destroy fat cell and reduce fatty transplanting success；And fat oil leaves Acid free fatty acid can be decomposed and be formed to fat cell, also become the factor for destroying normal tissue and normal fat cells and Fat transfer survival rate is reduced, and the formation of this free fatty acid can also cause and increase cell death, these necrotic tissues It is easy the probability for increasing superinfection or culturing base as bacterium, and fat oil can stay in the wall of fat cell and thin Intercellular interferes oxygen supply necessary to the existence of the fat cell of transplanting to cause fat cell downright bad as blood film.Because this A little reasons generally need to remove fat oil before fat transfer, but are influenced by operating condition and equipment, are still difficult to accomplish It is kept completely separate, still understands some and be mixed into transplant fat, the lower part in transplanting space is common when especially transplanting amount is more The fat oil of aggregation will affect surgical effect in this way, and the symptom of appearance includes causing inflammation or destroying tissue, form big lump, As lump etc. when touch.

In order to remove the red blood cell and fat oil that extract in fat, the method for most original is the fat utilization funnel that will be extracted The container of apperance stands natural separation, but is difficult fractionation of fatty oil in this way and (is located on fat after fat oil separation Layer), and elapsed time is relatively more.Another original processing method is to be squeezed to separate with hand after taking cloth to wrap in the fat of extraction Impurity, while the dynamics compressed fat by squeezing, achieve the purpose that separating and filtering goes out fat oil, but same flower in this way Take many times, and process is cumbersome, separating effect is related with operator, and prior this method has the hand of fat with people And the risk of infection after air contact.

It is some using the container of syringe form as separator for safely and effectively separating red corpuscle and fat oil The exploitation of technical research and relevant apparatus is reported successively.One kind is disclosed in Chinese invention patent application 200480002834.0 Closed loop fat transplantation system can see from the description of its specification, and the implant system is mainly for larger amount of fat transfer Operation achievees the purpose that bacterium infection, but each portion of the system will not occur in liposuction, liquid separation and fat transfer process Component relationship between part is relative complex, requires operation relatively high, is unfavorable for popularization and application, and the implant system is not examined Consider the separation problem of fat oil.A kind of autologous fat transplantation rouge is disclosed in Chinese utility model patent 2006220113167.x Fat cell collection screens syringe, inside and outside piston is arranged in syringe, it is complete to be formed in the tube negative pressure lacuna by fat suction machine At liposuction, then need to take out the interior outer piston, be inserted into the original annotation emitter push-pull rod with flap valve through special restructuring, through from The heart overflows grease by flap valve, then re-replaces the injection transplanting that syringe push-pull rod completes fat again.It can be seen that should Centrifugation is relied solely on to the separation of fat oil in patented technology, the fat oil of spilling is simply positioned at fatty top, and is difficult to separate Thoroughly, especially when only separating to a small amount of adipose tissue, accuracy is reduced, and to avoid being mixed into for fat oil, can only lose pure rouge Fat tissue causes the loss of fat.

A kind of device for being used to prepare adipose tissue and side are disclosed in Chinese invention patent application 201180034851.2 Method, apparatus structure is as shown in Fig. 1, including at least one washing and separation vessel 1, at least one described washing and separation are held Device 1 has the washing chamber 101 for washing liposuction materials, and the container 1 has entrance 102 and outlet 103, passes through for making Suction lipectomy and the leaflet fat material that extracts by the entrance 102 enter the washing chamber 101 and for making the material Fluid components in material leave the chamber 101 by the outlet 103, and the washing chamber 101 includes for passing through machine Tool stirs and is formed the device of the emulsion of fluid components, and emulsion forms device by least one agitating element of ball etc. 104 are constituted, for increasing the emulsification of the liquid component when the washing and separation vessel 1 are subjected to mechanically or manually stirring. It at least to include four steps as shown in Fig. 2 when carrying out the preparation of fat cell using the device, first is that washing step, it will Container 1 is maintained at vertical position in a manner of exporting 103 and face ground, by infusing the fluent material pressure of such as sterile saline It is mapped in the interior washing chamber 101 and is washed repeatedly, to make during obtaining no any oil, blood and any extraction The high-purity cell mass (Fig. 2 a) of solution；Second is that emulsifying step, is oriented in horizontal position for container, that is, is approximately parallel to The container 1 is manually or mechanically stirred on ground, to promote fluid components, specifically with the oil ingredient of fluids for sterilization substance With the emulsification (Fig. 2 b) of blood；Third is that container 1, is arranged in relatively by emulsion removing step with exporting 103 face-down modes In the vertical position on floor, the solid being contained on the emulsifying liquid liquid of the fat in washing chamber 101 is being constituted to obtain The layering of component, including the cell fragment, cell and the one or more cell mass that are floated on the emulsion of fluid components Solid component, by the emulsion of fluid components (that is, oil/liquid) by washing the outlet 103 with separation vessel 1 from washing chamber 101 discharges (Fig. 2 c)；Fourth is that solid component removing step, so that the outlet 103 of container 1 is vertically-oriented upward, so that solid component Swim in washing solution on (Fig. 2 d) to by solid component from washing and separation vessel be discharged.

The generation of emulsion is facilitated in foregoing invention patent application by the way that multiple beads are arranged in container as agitating device, To improve the purity of fat cell, still, since bead has certain quality, bottom is sunk to when container is placed vertically Portion is shaken in this case and is shaken, and the stirring action which is played is extremely limited, thus needs to cross in container It is stirred operation again, i.e., emulsifying step mentioned above, which increase operation difficulties, moreover, increasing if it is manual operation A possibility that having added pollution, then increases mechanical complexity if it is mechanically actuated, since it is desired that special be arranged so that container The rotation drive device of horizontal position is rotated to from vertical position.Meanwhile in entire fat cell preparation process, need to carry out At least three times container towards conversion operation, since container both ends are respectively connected with pipeline, repeatedly towards conversion operation the case where Under inevitably the soundness of the connecting portion of pipeline and container is had an impact, thus a possibility that increasing pollution. In addition, even if emulsifying step container is in horizontal positioned state, due to bead self gravity problem its in shaking/whipping process Container can still be focused more on to move close to the position on ground, there are difference in whole container for stirring action, thus newborn It is to be improved to change efficiency.

Thus, for above-mentioned technical problem of the existing technology, at present there are still can more easily carry out operation and It is more effectively carried out the preparation facilities of the fat cell of emulsification and the demand of method.

Summary of the invention

The purpose of the present invention is to provide a kind of devices that can be avoided drawbacks described above, for the fatty material by being pumped out Material such as leaflet fat, the fat material preparation i.e. comprising cell large crumb, cell and cell fragment are used for fatty group of transplanting It knits.

Another object of the present invention is the method for being related to preparing the adipose tissue for transplanting using described device, described Method and apparatus allow to maintain biomaterial in completely enclosed system, and by relatively simple operation step and relatively Efficient emulsification degree prepares the higher adipose tissue of degree of purification.

Another object of the present invention is to provide the adipose tissue prepared by above-mentioned apparatus and method, and especially fat is thin Born of the same parents.It contains less blood, oil, so as to keep better surgical effect after the implantation.

Above-mentioned purpose by including that the device of at least one washing and separation vessel is realized, at least one described washing and Separation vessel has the washing chamber of the fat material for washing suction, which has entrance and exit, for making fat Material passes through outlet by the fluid components that entrance enters washing chamber and makes the material and leaves the chamber, the washing Mixing component including at least one emulsion for being used to mechanically form fluid components in chamber, each mixing component include Elastomeric element and at least one sphere being fixed on elastomeric element.

Preferably, the mixing component is multiple.

Optionally, elastomeric element is fixed on washing and separation vessel inner wall.

Optionally, elastomeric element is fixed on washing and separation vessel roof and bottom wall, and/or, elastomeric element is fixed on institute State washing and separation vessel circumferential side wall.

Preferably, the mixing component is uniformly distributed in the circumferential around the longitudinal axis of the washing and separation vessel.

Optionally, elastomeric element one end is connected the sphere, and the other end is in free shape or is equally fixed with sphere.

Preferably, elastomeric element is helical spring, leaf spring and/or elastic rod.

Preferably, length of elastomeric element when static is more than or equal to the radius of container cross section.

Optionally, a sphere is set on each elastomeric element, and sphere is located at elastomeric element end.

Optionally, multiple spheres are set on each elastomeric element.

Preferably, multiple spheres are symmetrically laid along elastomeric element longitudinal axis.

Preferably, it is provided with size reducing apparatus in the washing chamber, for fat material to be processed to be cut into Smaller size.

Preferably, size reducing apparatus includes that at least one size reduces net.

Preferably, the size reducing apparatus includes that first size reduces net and the second size and reduces net, be located at into At mouthful and exit.

Preferably, the size reducing apparatus also include third size reduce net, be laid in first size reduce net and Second size reduces between net.

Preferably, the mesh that the size reduces net is gradually reduced by entrance to outlet.

The purpose of the present invention also realizes that the external member is used for comprising at least one by being used to prepare the external member of adipose tissue Prepare adipose tissue washing and separation vessel, for from patient's suction of fat material syringe, for collecting/injection treatment Biomaterial syringe, wash liquid container, the container for collecting obsolete material, and for connecting above-mentioned component Pipeline.

The purpose of the present invention also realizes that the device includes that washing and separation are held by being used to prepare the device of adipose tissue Device and holding meanss, the holding meanss provide the washing and separation appearance for keeping the washing and separation vessel Rotation of the device in the plane perpendicular to ground.

Preferably, the holding meanss also provide it is described washing and separation vessel in the plane perpendicular to ground along upper Lower and left and right directions vibration driving.

The purpose of the present invention further includes washing and the separation vessel obtained by being used to prepare adipose tissue as described above The adipose tissue or fat cell of preparation.

The present invention has the advantage that compared with the existing technology

1. since elastomeric element and sphere are connected, even if slight concussion and being rocked then when container is in a vertical state Also it can cause the movement of bead by a relatively large margin in container, and the overturning of spring causes indifference eddy motion, thus more effectively Ground promotes emulsion to be formed.Elastomeric element can be set to multiple, be evenly distributed on horizontal and vertical, this makes without vertical Moving up container can be realized more sufficient stirring, improve work efficiency.

2, since stirring action is mainly completed by sphere, impact is generated for the tissue in container and is rubbed main next Source is sphere, it is thus possible to which the integrality for farthest guaranteeing fat cell reduces the breakage rate of fat cell.

3, sufficient stirring can be realized in the simple movement in view of container, it is thus possible to which on the one hand streamline operation drops On the other hand contaminated risk when low manual operation can also reduce complexity mechanical under Mechanical Driven operational circumstances.

4, it completes to stir without horizontal container due to the presence of elastomeric element, thus allows in container longitudinal middle part, example As added one or more sizes reduction nets close to entrance namely positioned at the position of agitating element upstream, so as to guarantee wait locate The size for managing material reduces degree and effect, further increases working efficiency.

Detailed description of the invention

It, below will be to required in embodiment or description of the prior art in order to illustrate more clearly of the technical solution of the application The attached drawing used is briefly described, it should be apparent that, the drawings in the following description are only some examples of the present application, right For those of ordinary skill in the art, without creative efforts, it can also be obtained according to these attached drawings Its attached drawing.

Fig. 1 shows the washing of the preparation facilities of adipose tissue in the prior art and the side view of separation vessel.

Fig. 2 shows the operation streams that adipose tissue is prepared based on the preparation facilities comprising washing shown in FIG. 1 and separation vessel Journey schematic diagram.

Fig. 3 shows the washing of first embodiment according to the invention and the vertical section schematic diagram of separation vessel.

Fig. 4 shows the washing of second embodiment according to the invention and the vertical section schematic diagram of separation vessel.

Fig. 5 shows the washing of third embodiment according to the invention and the vertical section schematic diagram of separation vessel.

Fig. 6 shows the washing of the 4th embodiment according to the invention and the vertical section schematic diagram of separation vessel.

Fig. 7 shows the washing of the 5th embodiment according to the invention and the vertical section schematic diagram of separation vessel.

Fig. 8 shows the fatty preparation facilities external member comprising washing and separation vessel according to the embodiment of the present invention and illustrates Figure.

Fig. 9 is the method flow diagram that adipose tissue is prepared using the fatty preparation facilities of embodiments of the present invention.

Specific embodiment

Below in conjunction with Figure of description, the technical scheme in the embodiment of the application is clearly and completely described, shows So, described embodiments are only a part of embodiments of the present application, instead of all the embodiments.Based on the reality in the application Apply example, those of ordinary skill in the art's every other embodiment obtained without creative labor, all Belong to the range of the application protection.

<washing and separation vessel>

<first embodiment>

Fig. 3 shows the washing of first embodiment according to the invention and the vertical section schematic diagram of separation vessel.Washing and Separation vessel 1 has the washing chamber 101 of the material for washing suction, and the container 1 has entrance 102 and outlet 103, uses In making the fat material that gets of suction enter washing chamber 101 and sequentially in time by entrance 102, i.e. fluid group first Divide and the chamber 101 is left by outlet 103 with the sequence of then solid component, the washing chamber 101 is including being used for machinery Ground forms the cream of fluid components, the oil specifically obtained from the fat cell of rupture, blood and/or other sterilised liq solution Change the device of liquid.

The emulsion forms device and is made of at least one mixing component 4, at least one described mixing component 4 includes bullet Property the component 41 and sphere 42 that is fixed on elastomeric element 41, specifically, elastomeric element 41 can be helical spring.Optionally, Elastomeric element 41 is affixed to the top and bottom of washing and separation vessel 1.Sphere 42 is fixed on elastomeric element 41, for making Increase the emulsion of liquid component when washing and separation vessel 1 are subjected to shaking, rocking.In the apparatus of the present, the letter of container 1 The dynamic stirring of one hand is capable of providing the separation of liquid phase and solid phase, and the liquid phase includes fluid emulsion, and solid phase obtained includes thin Born of the same parents, cell fragment, cell mass.

Optionally, the quantity of mixing component 4 is more than or equal to 4.

Optionally, mixing component 4 is uniformly distributed in container 1.For example, symmetrical in the center line of short transverse with container 1 It is distributed in the bottom wall and roof of container, and/or, it is circumferentially distributed centered on the longitudinal axis of container 1.

Optionally, it is provided with size reducing apparatus in washing and separation vessel 1, which includes several rulers Very little reduction net.First size at least, which is provided with, at the entrance 102 of washing and separation vessel 1 reduces net 301.Material to be processed Net 301 is reduced by the size before entering the washing chamber 101 of washing and separation vessel 1, it is smaller to cut the material into Size.Optionally, the second size is also equipped at washing and the outlet 103 of separation vessel 1 reduce net 302.

Preferably, from entrance 102 to outlet 103, the mesh size that size reduces net successively decreases.

Size reduces net 3 and is made of at least one set of parallel or intersection sheet material or cutting line.Preferably, it the sheet material or cuts Secant is made of pasteurization material such as metal.

The mesh that the size reduces net 34 can have various shape, such as, but not limited to: triangle, rectangle, water chestnut Shape, hexagon, octagon, etc..

<second embodiment>

Washing and the separation vessel of second embodiment according to the invention are as shown in figure 4, its structure and the first embodiment party Formula is similar, the difference is that, the elastomeric element 41 of helical spring form is affixed to the side wall of washing and separation vessel 1.

Optionally, mixing component is alternately laid in the opposite side of 1 inner wall of washing and separation vessel.

Optionally, helically form is laid on the side wall of washing and separation vessel 1 mixing component.

In this embodiment, optionally, reduce net 301 in first size and the second size reduces between net 302, compared to 103 are exported closer to the position of entrance 102, third size is set and reduces net 303.

Material to be processed reduces net by two-stage size before entering the washing chamber 101 of washing and separation vessel 1 301,303, so as to cut the material into smaller size.

<third embodiment>

Washing and the separation vessel of third embodiment according to the invention are as shown in figure 5, its structure and the first embodiment party Formula is similar (size reduces net omit is not shown), the difference is that, the elastomeric element 41 of helical spring form not with washing and Separation vessel 1 connects, but has free-end.In this embodiment, elastomeric element 41 is provided fills between the inner wall of container 1 Point bounce so that either container 1 be in be vertically oriented to still horizontally toward when, the shaking of container 1 can cause The active movement of sphere 42, to effectively improve emulsifying effectiveness.

Optionally, 41 both ends of elastomeric element of helical spring form are provided with sphere 42.

In this embodiment, optionally, reducing appointing between net 301 and the second size reduction net 302 in first size One position adds 1-3 size and reduces net, thus further increases size and reduces effect, ensures the segregation ratio of adipose tissue.

<the 4th embodiment>

Washing and the separation vessel of 4th embodiment according to the invention are as shown in fig. 6, its structure and the first embodiment party Formula is similar (size reduces net omission and is not shown), the difference is that, the elastomeric element 41 ' that mixing component 4 ' is included is piece Spring, the elastomeric element 41 ' of leaf spring form are affixed to container inner wall, and sphere 42 ' is affixed to elastomeric element 41 '.

Optionally, the elastomeric element 41 ' of leaf spring form is affixed to the upper and lower ends of washing and separation vessel 1 or is solid It is connected on its inner wall.

In this embodiment, the direction for being used as the leaf spring of elastomeric element 41 ' can be identical, for example, leaf spring area Biggish one side can also be different both facing to some direction, for example, the biggish one side of leaf spring area is both facing to chamber wall, or Person is directed towards random arrangement.

Optionally, the length of leaf spring can be identical, can also be different from each other, for example, being in the alternate form of length, either The form spirally lengthened.

<the 5th embodiment>

Washing and the separation vessel of 5th embodiment according to the invention are as shown in fig. 7, its structure and the first embodiment party Formula is similar (size reduces net omission and is not shown), the difference is that, mixing component 4 " including elastomeric element 41 " and sphere 42 ", Wherein, each elastomeric element 41 " on be fixed with more than one sphere 42 ".

Optionally, elastomeric element 42 " is helical spring, is affixed to 1 inner wall of washing and separation vessel, or be free on In washing chamber.

Optionally, the same side or not ipsilateral of more than one sphere 42 " being affixed to same elastomeric element 42 ".

Optionally, it is connect between sphere 42 " with elastomeric element 42 " by short silk.

<adipose tissue preparation facilities and external member>

Fig. 8 shows showing for the fatty preparation facilities external member comprising washing and separation vessel according to the embodiment of the present invention It is intended to.The external member allows the transfer of fat material that can occur in closed system, from the rouge of donor in the closed system Fat material and final adipose tissue obtained do not contact external environment completely.

The external member includes the washing described according to above embodiment and separation vessel 1, for from patient's sucked material Syringe 7, for collect/syringe 12 of the biomaterial of injection treatment, wash liquid container 10 is (for example, accommodate salt water Sack), the container 11 for collecting obsolete material, they by 7 sterilization pipes, changeover valve, luer connector connection or can connect It is connected to together.

Specifically, washing and 1 entrance 102 of separation vessel and/or outlet 103 can be connected to three-way valve 9, such as existing skill Known Luer lock syringe 7 is connected to the washing equipped with three-way valve 9 and the entrance of separation vessel 1 by pipe or conduit 8 in art 102, which carries out the suction of fat material via the intubation for being equipped with one or more holes on the surface thereof.

The liquid that such as physiological saline is accommodated in wash liquid sack 10, be used for carry out fat material washing and The air in container 1 is excluded before separation, offer pressure forces fat material to enter and leave chamber 101 and to fat material It is washed repeatedly.

Syringe 12 is connected to outlet 103, for collecting the prepared biomaterial mixed with liquid, is allowed to sink It forms sediment to obtain the separation of solid component and remaining liquid component.The material of storage within the container 12 can be designed to final Reduce in container 2 before saving and/or injecting again in washing and separation vessel 1 and/or size and is handled again.

For collecting the container such as sack 11 of obsolete material by 9 connection outlet 103 of three-way valve, by emulsified liquid collection its In.

Washing and separation vessel 1 can be kept by a holding meanss (not shown), and fortune needed for providing the container 1 Dynamic driving, for example, rotation of the container 1 in the plane perpendicular to ground is capable of providing, to realize by exporting 103 towards ground Converted to entrance 102 towards the inversion on ground, meanwhile, can also provide upper and lower in the plane perpendicular to ground of container 1 and/or The vibration driving of left and right.Optionally, holding meanss can also provide container 1 around the rotation of the axis perpendicular to ground.

<adipose tissue preparation flow>

Fig. 9 shows the method flow diagram that adipose tissue is prepared using the fatty preparation facilities of embodiments of the present invention.

Specifically, in step 501, i.e. size reduction step, by gravity/injection via the liquid in wash liquid container 10 Body provides pressure, so that aspirating resulting leaflet fat passes through size reducing apparatus at least once, to separate cell mass, makes The cell mass is obtained as predetermined size greatly or less than predetermined size, and the cell mass is had on an average Roughly the same size.

In step 502, i.e. washing and separating step, disinfecting cleaning solution is injected into the washing of washing and separation vessel 1 In chamber 101 at least once, the washing and separation vessel 1 are stirred, promotes fluid components to form device by emulsion Emulsification specifically promotes the emulsification of oil ingredient and fluids for sterilization substance, obtains layering of the solid component on emulsifying liquid liquid, The layering of the solid component includes the solid component of cell fragment, cell and one or more cell masses；By it is described enter Mouth 102 injects clean washing solution, and passes through the outlet 103 and fluid group of the washing and separation vessel 1 The removing of the density gradient of the emulsion divided acts on and the emulsion of the fluid components is discharged from the washing chamber 101. The washing and separating step are repeated, until the fluid components of discharge are without such as any impurity of oil and/or blood.

In step 503, i.e., step is discharged in solid component, the outlet 103 upward in the case where by the washing and point It vertically positions from container 1, to allow the solid component to swim on the washing solution, is held by the washing and separation The entrance 102 of device 1 under pressure injects washing solution, to allow the solid component to be discharged from outlet 103.

Wherein, the washing solution injecting step, the emulsion are discharged step and for generating the emulsion Whipping step is to be disposed in vertical position in the container 1, and the outlet 103 is downward, i.e., the feelings towards ground It is carried out under condition.

Optionally, the whipping step for generating the emulsion is in the container 1 by relative to surface water level land cloth It is carried out in the case where setting.

Allow to obtain transplantable adipose tissue/fat by simple, rapid and low price processing by above-mentioned processing thin Born of the same parents can directly inject to patient, or be stored under the suitable conditions.

Device disclosed above, external member and the method for forming subject of the present invention can significantly be applied not only to prepare wait move The adipose tissue of plant, but also be used to prepare need to have high level of purity for use any kind of tissue/cell/it is thin Born of the same parents' agglomerate.

Those of ordinary skill in the art it should be appreciated that more than embodiment be intended merely to illustrate the present invention, And be not used as limitation of the invention, as long as the change in spirit of the invention, to embodiment described above Change, modification will all be fallen in scope of the presently claimed invention.

Claims (10)

1. being used to prepare washing and the separation vessel (1) of adipose tissue, there is the washing chamber for washing fat material (101), entrance (102) and outlet (103), for making fat material enter washing chamber (101) by entrance (102) and making The fluid components of the material pass through the outlet (103) and leave the washing chamber (101), which is characterized in that the washing Mixing component (4,4 ', 4 ") including at least one emulsion for being used to mechanically form fluid components in chamber (101), each Mixing component (4,4 ', 4 ") comprising elastomeric element (41,41 ', 41 ") and is fixed on elastomeric element (41,41 ', 41 ") extremely A few sphere (42,42 ', 42 ").

2. washing and the separation vessel (1) of adipose tissue are used to prepare as described in claim 1, wherein the mixing component is It is multiple.

3. being used to prepare washing and the separation vessel (1) of adipose tissue as described in claim 1, wherein the elastomeric element (41,41 ', 41 ") are fixed on the washing and separation vessel (1) inner wall.

4. being used to prepare washing and the separation vessel (1) of adipose tissue as claimed in claim 3, wherein the elastomeric element (41,41 ', 41 ") are fixed on the washing and separation vessel (1) roof and bottom wall, and/or, the elastomeric element (41,41 ', 41 " washing and separation vessel (1) circumferential side wall) are fixed on.

5. washing and the separation vessel (1) of adipose tissue are used to prepare as claimed in claim 4, wherein the mixing component (4) longitudinal axis around the washing and separation vessel (1) is uniformly distributed in the circumferential.

6. being used to prepare washing and the separation vessel (1) of adipose tissue as described in claim 1, wherein the elastomeric element (41,41 ', 41 ") one end is connected the sphere (42,42 ', 42 "), the other end be in free shape or be equally fixed with sphere (42, 42 ', 42 ").

7. such as the washing of any of claims 1-6 for being used to prepare adipose tissue and separation vessel (1), wherein institute Stating elastomeric element (41,41 ', 41 ") is helical spring, leaf spring and/or elastic rod.

8. washing and the separation vessel (1) of adipose tissue are used to prepare as claimed in claim 7, wherein the elastomeric element The length of (41,41 ', 41 ") when static is more than or equal to the radius of container cross section.

9. the washing according to any one of claims 1 to 5 for being used to prepare adipose tissue and separation vessel (1), wherein every One sphere (42,42 ') is set on a elastomeric element (41,41 '), and the sphere (42,42 ') is located at the elasticity Component (41,41 ') end.

10. such as the washing of any of claims 1-6 for being used to prepare adipose tissue and separation vessel (1), wherein every Multiple spheres (42 ', 42 ") are set on a elastomeric element (41 ', 41 ").