CN109266532B

CN109266532B - Washing and separating vessel for preparing adipose tissue

Info

Publication number: CN109266532B
Application number: CN201811153352.5A
Authority: CN
Inventors: 邓贵澧; 徐靖宁; 黄君杰
Original assignee: Zhejiang Quantum Medical Devices Co Ltd
Current assignee: Zhejiang Quantum Medical Devices Co Ltd
Priority date: 2018-09-30
Filing date: 2018-09-30
Publication date: 2022-07-01
Anticipated expiration: 2038-09-30
Also published as: CN109266532A

Abstract

The present invention relates to a container, a device and a kit for preparing adipose tissue from aspirated fatty material, which can be used for injection. In particular, a washing and separating container for preparing adipose tissue has a washing chamber for washing aspirated fatty material, the container having an inlet and an outlet for the fatty material to enter the washing chamber through the inlet and for the fluid component of said material to leave said chamber through the outlet, said washing chamber comprising inside it at least one stirring assembly for mechanically forming an emulsion of the fluid component, each stirring assembly comprising an elastic member and at least one ball attached to the elastic member. Because the elastic component is fixedly connected with the ball body, slight vibration and shaking can also cause the small ball in the container to move to a larger extent, and the turning of the spring causes undifferentiated eddy current movement, thereby more effectively promoting the formation of emulsion, simplifying the operation process and improving the working efficiency.

Description

Washing and separating container for preparing adipose tissue

Technical Field

The present invention relates to a container, a device and a kit for preparing adipose tissue from aspirated fatty material, which can be used for injection.

Background

As early as the 70's of the last century, autologous fat transplantation has become a method of confirmation in the plastic surgery world, and has been gradually applied to various fields of cosmetic plastic surgery. Autologous fat transplantation requires treatment of the sucked fat, and it is important that red blood cells in the sucked fat and fatty oil components produced from the crushed fat cells must be discharged first. This is because the components after the decomposition of erythrocytes destroy normal tissues, thereby destroying adipocytes and reducing the survival rate of transplantation of fats; while fatty oil is separated from fat cells, it decomposes and forms acidic free fatty acids, which also become a factor for destroying normal tissues and normal fat cells to reduce the survival rate of fat transplantation, and the formation of free fatty acids also causes and increases cell death, which easily increases the probability of secondary infection or becomes a culture base of bacteria, and the fatty oil can remain on the wall and cells of fat cells, like blood membranes, to prevent the supply of oxygen necessary for the survival of transplanted fat cells, resulting in the necrosis of fat cells. For these reasons, it is generally necessary to remove the fatty oil before the fat transplantation, but it is still difficult to completely separate the fat due to the operation conditions and facilities, or a part of the fat is mixed into the transplanted fat, especially the fatty oil which is frequently accumulated in the lower part of the transplanted space when the transplanted amount is large, which may affect the operation effect, and the symptoms include causing inflammation or destroying tissues, forming a large lump, being touched like the lump, etc.

In order to remove erythrocytes and fatty oil from the extracted fat, the most original method is to naturally separate the extracted fat by standing the extracted fat in a funnel-shaped container, but the separation of fatty oil (the fat is separated and then located on the upper layer of the fat) is difficult and time-consuming in this method. Another original treatment method is to pack the extracted fat with cloth and then squeeze the fat with hands to separate impurities, and simultaneously compress the fat by the squeezing force to achieve the purpose of separating and filtering out the fat oil, but the method also takes a lot of time, is complicated in process, has a separation effect related to operators, and more importantly, has the risk of infection after the fat is contacted with hands of people and air.

In order to separate red blood cells and fatty oil safely and efficiently, some technical developments using a syringe-shaped container as a separation device and developments of related devices have been successively reported. The chinese patent application 200480002834.0 discloses a closed loop fat transplantation system, which can be seen from the description of the specification, and the transplantation system mainly aims at a large amount of fat transplantation operations to achieve the purpose that bacterial infection does not occur in the processes of fat extraction, liquid separation and fat transplantation, but the composition relationship among the components of the system is relatively complex, the operation requirement is relatively high, and the popularization and application are not facilitated, and the transplantation system does not consider the problem of fat oil separation. Chinese utility model patent 2006220113167 discloses an autologous fat transplantation adipocyte gathers screening syringe in x, sets up inside and outside piston in the syringe, forms negative pressure lacuna and accomplishes the liposuction through the liposuction machine in intraductal, later need take out this inside and outside piston, insert the former syringe push-and-pull rod that has the flap through special transformation, make the grease spill over through the flap through the centrifugation, then change the syringe push-and-pull rod again and accomplish the injection transplantation of fat. It can be seen that the separation of the fatty oil in the patent technology only depends on centrifugation, the spilled fatty oil is only located at the upper part of the fat and is difficult to separate completely, especially when only a small amount of fat tissue is separated, the accuracy is reduced, and only pure fat tissue can be lost to avoid the mixing of the fatty oil, so that the loss of the fat is caused.

Chinese patent application 201180034851.2 discloses an apparatus and method for preparing adipose tissue, the device structure is shown in figure 1, and comprises at least one washing and separating container 1, wherein the at least one washing and separating container 1 is provided with a washing chamber 101 for washing liposuction materials, the container 1 is provided with an inlet 102 and an outlet 103, for allowing lobular fatty material extracted by fat suction to enter the washing chamber 101 through the inlet 102 and for allowing fluid components in the material to leave the chamber 101 through the outlet 103, and said washing chamber 101 comprises means for forming an emulsion of the fluid components by mechanical agitation, the emulsion forming means being constituted by at least one agitation element 104, such as a ball or the like, for increasing the emulsification of said liquid components when said washing and separating vessel 1 is subjected to mechanical or manual agitation. When the device is used for the preparation of adipocytes, as shown in figure 2, it comprises at least four steps, one being a washing step, the container 1 is kept in vertical position with the outlet 103 facing the ground, repeated washing is carried out by pressure injection of a liquid material, such as sterile saline, into said inner washing chamber 101, to obtain a high-purity cell mass free from any oil, blood and any solution used during the extraction (figure 2 a); secondly, an emulsifying step, in which the container is oriented in a horizontal position, i.e. approximately parallel to the ground, said container 1 being stirred manually or mechanically to promote the emulsification of the fluid components, in particular the oil component with the sterile fluid substance and the blood (fig. 2 b); a third emulsion removal step, arranging the container 1 in a vertical position with respect to the floor with the outlet 103 facing downwards, to obtain stratification of the solid components, including the cell debris, the cells and the solid component of the one or more cell agglomerates floating on the emulsion of fluid components, on the liquid emulsion constituting the fat contained in the washing chamber 101, discharging the emulsion of fluid components (i.e. the oil/liquid) from the washing chamber 101 through the outlet 103 of the washing and separating container 1 (fig. 2 c); fourthly, the solid component removal step, such that the outlet 103 of the vessel 1 is oriented vertically upwards, such that the solid component floats on the wash solution (fig. 2d) to discharge the solid component from the wash and separation vessel.

The above-mentioned patent application of the invention promotes the production of the emulsion by arranging a plurality of beads as stirring means in the container, thereby improving the purity of the fat cells, however, since the beads have a certain mass and sink to the bottom when the container is placed vertically, in this case, they vibrate and shake, and the stirring action of the beads is very limited, it is necessary to perform the stirring operation after the container is moved laterally, i.e., the above-mentioned emulsification step, which increases the operation difficulty, and if it is a manual operation, the possibility of contamination is increased, and if it is a mechanical operation, the mechanical complexity is increased, because a rotation driving means for rotating the container from the vertical position to the horizontal position needs to be specially arranged. Meanwhile, in the whole adipose cell preparation process, at least three times of orientation conversion operations of the container are required, and because the two ends of the container are both connected with the pipelines, the stability of the connecting part of the pipelines and the container is inevitably affected under the condition of multiple orientation conversion operations, so that the pollution possibility is increased. In addition, even if the container is horizontally placed in the emulsification step, the small balls can still move more intensively at the position close to the ground in the shaking/stirring process due to the gravity of the small balls, and the stirring action is different in the whole container, so that the emulsification efficiency needs to be improved.

Therefore, in view of the above technical problems of the prior art, there is still a need for an apparatus and method for preparing adipocytes that can be more conveniently operated and more efficiently emulsified.

Disclosure of Invention

The object of the present invention is to provide a device for preparing adipose tissue for transplantation from aspirated fatty material, such as lobular fat, i.e. fatty material containing large clumps of cells, cells and cell debris, which avoids the above-mentioned drawbacks.

It is a further object of the present invention to relate to a method for preparing adipose tissue for transplantation using the device, which allows maintaining biological materials in a completely closed system and preparing adipose tissue with a higher degree of purification through relatively simple operation steps and a relatively high degree of emulsification.

It is a further object of the present invention to provide adipose tissue, in particular adipocytes, prepared by the above-described device and method. It contains less blood and oil, and thus can maintain better surgical effect after implantation.

The above object is achieved by a device comprising at least one washing and separating vessel having a washing chamber for washing sucked fatty material, the vessel having an inlet and an outlet for fatty material entering the washing chamber through the inlet and for fluid components of said material leaving said chamber through the outlet, at least one agitation assembly for mechanically forming an emulsion of the fluid components being comprised in said washing chamber, each agitation assembly comprising an elastic member and at least one ball attached to the elastic member.

Preferably, the stirring assembly is plural.

Optionally, the resilient member is attached to the inner wall of the washing and separating vessel.

Optionally, the resilient members are attached to the top and bottom walls of the washing and separating vessel and/or the resilient members are attached to the circumferential side walls of the washing and separating vessel.

Preferably, the agitator assemblies are evenly circumferentially arranged about the longitudinal axis of the washing and separating vessel.

Optionally, one end of the elastic component is fixedly connected with the sphere, and the other end of the elastic component is in a free shape or is fixedly connected with the sphere.

Preferably, the resilient member is a helical spring, a leaf spring and/or a resilient rod.

Preferably, the length of the elastic member at rest is equal to or greater than the radius of the cross section of the container.

Optionally, one ball is provided on each resilient member, the ball being located at an end of the resilient member.

Optionally, a plurality of spheres are disposed on each resilient member.

Preferably, the plurality of spheres is symmetrically arranged along the longitudinal axis of the resilient member.

Preferably, size reduction means are provided inside the washing chamber for cutting the fatty material to be treated into smaller sizes.

Preferably, the size reduction means comprises at least one size reduction mesh.

Preferably, the size reduction means comprises a first size reduction mesh and a second size reduction mesh, located at the inlet and the outlet, respectively.

Preferably, the size reduction device further comprises a third size reduction mesh disposed between the first size reduction mesh and the second size reduction mesh.

Preferably, the mesh openings of the size reduction net are gradually reduced from the inlet to the outlet.

The object of the invention is also achieved by a kit for preparing adipose tissue comprising at least one washing and separating container for preparing adipose tissue, a syringe for aspirating adipose material from a patient, a syringe for collecting/injecting processed biological material, a washing liquid container, a container for collecting waste material, and a tubing for connecting the above components.

The object of the invention is also achieved by a device for preparing adipose tissue, comprising a washing and separating vessel, and a holding device for holding the washing and separating vessel and providing a rotation of the washing and separating vessel in a plane perpendicular to the ground.

Preferably, the holding means also provides for vibratory driving of the washing and separating container in up-down and left-right directions in a plane perpendicular to the ground.

It is also an object of the present invention to obtain adipose tissue or adipocytes prepared by the washing and separating vessel for preparing adipose tissue as described above.

Compared with the prior art, the invention has the following advantages:

1. since the elastic member is fixedly connected to the ball, even when the container is in an upright state, slight shaking and shaking causes a large movement of the ball in the container, and the turning of the spring causes an undifferentiated swirling motion, thereby more effectively promoting emulsion formation. The elastic components can be arranged in a plurality of numbers and are uniformly distributed in the transverse direction and the longitudinal direction, so that the container does not need to be moved in the longitudinal direction to realize sufficient stirring, and the working efficiency is improved.

2, because the stirring effect is mainly completed by the spheres, the spheres are the main sources for generating impact and friction on tissues in the container, the integrity of the fat cells can be ensured to the maximum extent, and the damage rate of the fat cells is reduced.

3, sufficient stirring can be realized by simple movement of the container, so that the operation process can be simplified, and the pollution risk in manual operation is reduced, and the mechanical complexity in mechanical driving operation is reduced.

4. The presence of the elastic means, without the need to stir transversely to the container, allows to add one or more size reduction meshes in the longitudinal middle of the container, for example close to the inlet, i.e. upstream of the stirring element, thus making it possible to guarantee the degree and effect of size reduction of the material to be treated, further increasing the working efficiency.

Drawings

In order to more clearly illustrate the technical solutions of the present application, the drawings needed for the description of the embodiments or the prior art will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present application, and it is obvious for those skilled in the art to obtain other drawings without creative efforts.

Fig. 1 shows a side view of a washing and separating vessel of a prior art adipose tissue preparation device.

Fig. 2 shows a schematic flow diagram of an operation for preparing adipose tissue based on a preparation device comprising the washing and separating vessel shown in fig. 1.

Fig. 3 shows a schematic longitudinal section of a washing and separating vessel according to a first embodiment of the invention.

Fig. 4 shows a schematic longitudinal section of a washing and separating vessel according to a second embodiment of the invention.

Fig. 5 shows a schematic longitudinal section through a washing and separating vessel according to a third embodiment of the invention.

Fig. 6 shows a schematic longitudinal section through a washing and separating vessel according to a fourth embodiment of the invention.

Fig. 7 shows a schematic longitudinal section of a washing and separating vessel according to a fifth embodiment of the invention.

Fig. 8 shows a schematic view of a fat preparation device kit comprising a washing and separating vessel according to an embodiment of the present invention.

Fig. 9 is a flowchart of a method of preparing adipose tissue using the fat preparation device according to the embodiment of the present invention.

Detailed Description

The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings of the specification, and it is obvious that the described embodiments are only a part of the embodiments of the present application, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present application.

< washing and separating vessel >

< first embodiment >

Fig. 3 shows a schematic longitudinal section of a washing and separating vessel according to a first embodiment of the invention. The washing and separating container 1 has a washing chamber 101 for washing the sucked material, said container 1 having an inlet 102 and an outlet 103 for the sucked fatty material to enter the washing chamber 101 through the inlet 102 and to leave said chamber 101 through the outlet 103 in a time sequence, i.e. in a sequence of first a fluid component and then a solid component, said washing chamber 101 comprising means for mechanically forming an emulsion of the fluid component, in particular of an oil, blood and/or other sterile liquid solution obtained from ruptured fat cells.

The emulsion forming device consists of at least one stirring assembly 4, the at least one stirring assembly 4 comprising an elastic member 41 and a ball 42 attached to the elastic member 41, in particular, the elastic member 41 may be a coil spring. Optionally, the elastic members 41 are secured to the top and bottom of the washing and separating vessel 1. The spheres 42 are attached to the resilient member 41 for increasing the emulsion of the liquid component when the washing and separating vessel 1 is subjected to shaking. In the device of the invention, simple manual stirring of the container 1 can provide separation of the liquid phase, comprising the fluid emulsion, from the solid phase, comprising the cells, the cell fragments, the cell clumps, obtained.

Optionally, the number of stirring assemblies 4 is equal to or greater than 4.

Alternatively, the stirring assemblies 4 are evenly distributed within the container 1. For example, symmetrically distributed about the centre line of the container 1 in the height direction on the bottom and top walls of the container, and/or circumferentially distributed about the longitudinal axis of the container 1.

Optionally, a size reduction device is provided in the washing and separating vessel 1, which size reduction device comprises several size reduction meshes. At least at the inlet 102 of the washing and separating vessel 1 a first size reduction mesh 301 is arranged. The material to be treated passes through the size reduction mesh 301 before entering the washing chamber 101 of the washing and separating container 1 to cut the material into smaller sizes. Optionally, a second size reduction mesh 302 is also provided at the outlet 103 of the washing and separating vessel 1.

Preferably, the mesh size of the size reduction mesh decreases from the inlet 102 to the outlet 103.

The size-reducing net 3 is made up of at least one set of parallel or intersecting sheets or cut lines. Preferably, the sheet or cut line is made of a sterilizing material such as metal.

The mesh openings of the size reduction mesh 34 may have various shapes, such as, but not limited to: triangular, rectangular, diamond, hexagonal, octagonal, etc.

< second embodiment >

A washing and separating vessel in accordance with a second embodiment of the present invention is shown in fig. 4, and has a structure similar to that of the first embodiment except that an elastic member 41 in the form of a coil spring is fixedly attached to a sidewall of the washing and separating vessel 1.

Alternatively, the agitating members are alternately disposed at opposite sides of the inner wall of the washing and separating vessel 1.

Alternatively, the stirring assembly is arranged in a spiral form on the side wall of the washing and separating vessel 1.

In this embodiment, optionally, a third size reduction mesh 303 is provided between the first size reduction mesh 301 and the second size reduction mesh 302, at a position closer to the inlet 102 than to the outlet 103.

The material to be treated passes through the two stage size reduction meshes 301, 303 before entering the washing chamber 101 of the washing and separating vessel 1, whereby the material can be cut to smaller sizes.

< third embodiment >

A washing and separating vessel in accordance with a third embodiment of the invention is shown in fig. 5 and is of similar construction to the first embodiment (size reduction net not shown) except that the resilient member 41 in the form of a helical spring is not connected to the washing and separating vessel 1 but has a free end. In this embodiment, the elastic member 41 provides a sufficient repulsive force with the inner wall of the container 1, so that shaking of the container 1 can cause active movement of the ball 42 regardless of whether the container 1 is in a vertical or horizontal orientation, thereby effectively improving the emulsifying effect.

Optionally, both ends of the elastic member 41 in the form of a coil spring are provided with balls 42.

In this embodiment, optionally, 1 to 3 size-reducing meshes are additionally provided at any position between the first size-reducing mesh 301 and the second size-reducing mesh 302, thereby further improving the size-reducing effect and securing the separation ratio of the adipose tissues.

< fourth embodiment >

A washing and separating vessel in accordance with a fourth embodiment of the present invention is shown in fig. 6, and has a similar structure to that of the first embodiment (size reduction net is omitted from illustration), except that the agitating assembly 4 ' includes an elastic member 41 ' which is a leaf spring, the elastic member 41 ' in the form of a leaf spring is fixedly attached to the inner wall of the vessel, and a ball 42 ' is fixedly attached to the elastic member 41 '.

Alternatively, elastic members 41' in the form of leaf springs are attached to both upper and lower ends of the washing and separating vessel 1, or to the inner wall thereof.

In this embodiment, the orientation of the leaf springs used as the elastic members 41' may be the same, for example, the surfaces of the leaf springs having a larger area face a certain direction, or may be different, for example, the surfaces of the leaf springs having a larger area face the container wall, or may be randomly arranged.

Alternatively, the leaf springs may be of the same length or of different lengths, for example in alternating lengths or in a helically extended form.

< fifth embodiment >

A washing and separating vessel according to a fifth embodiment of the invention is shown in fig. 7 and is of similar construction to the first embodiment (size reduction net not shown) except that the agitator assembly 4 "comprises resilient members 41" and spheres 42 ", wherein more than one sphere 42" is attached to each resilient member 41 ".

Alternatively, the elastic member 42 "is a helical spring, which is either attached to the inner wall of the washing and separating container 1 or is free in the washing chamber.

Optionally, more than one ball 42 "is attached to the same side or different sides of the same resilient member 42".

Optionally, the ball 42 "is connected to the resilient member 42" by a short wire.

< adipose tissue preparation device and kit >

Fig. 8 shows a schematic view of a fat preparation device kit comprising a washing and separating vessel according to an embodiment of the present invention. The kit allows the transfer of fatty material to take place in a closed system where the fatty material from the donor and the finally prepared fatty tissue are completely free from the external environment.

The kit comprises a washing and separating container 1 as described in the above embodiments, a syringe 7 for aspirating material from a patient, a syringe 12 for collecting/injecting processed biological material, a washing liquid container 10 (e.g. a bag containing saline), a container 11 for collecting waste material, which are connected or can be connected together by a 7 sterile tube, a multi-way valve, a luer connector.

In particular, the inlet 102 and/or the outlet 103 of the washing and separating vessel 1 can be connected to a three-way valve 9, such as a luer lock syringe 7 known in the art connected by a tube or duct 8 to the inlet 102 of the washing and separating vessel 1 equipped with the three-way valve 9, the syringe 7 performing the aspiration of the fatty material via a cannula provided with one or more holes on its surface.

The washing liquid bag 10 contains a liquid, such as physiological saline, which is used to evacuate air from the container 1, provide pressure to force the fatty material into and out of the chamber 101, and to repeatedly wash the fatty material before washing and separating the fatty material.

The syringe 12 is connected to an outlet 103 for collecting the prepared biological material mixed with the liquid, which is allowed to settle to obtain separation of the solid component from the residual liquid component. The material stored in the container 12 may be designed to be reprocessed in washing and separating the container 1 and/or size reducing container 2 prior to final storage and/or re-injection.

A container for collecting waste material, such as a bag 11, is connected to the outlet 103 via the three-way valve 9, and the emulsion is collected therein.

The washing and separating vessel 1 may be held by a holding means (not shown) and provide for the driving of the desired movement of the vessel 1, for example to provide for the rotation of the vessel 1 in a plane perpendicular to the ground to effect the inversion of the vessel from the outlet 103 towards the ground to the inlet 102 towards the ground, and also to provide for the oscillating driving of the vessel 1 up and down and/or side to side in a plane perpendicular to the ground. Alternatively, the holding means may also provide rotation of the container 1 about an axis perpendicular to the ground.

< procedure for adipose tissue preparation >

Fig. 9 shows a flowchart of a method for preparing adipose tissue using the fat preparation device according to the embodiment of the present invention.

Specifically, in step 501, the size reduction step, pressure is provided by gravity/injection via the liquid within the washing liquid container 10, so that the resulting lobular fat is sucked through the size reduction device at least once, thereby separating the cell pellet so that it is as large or smaller than a predetermined size and so that it has on average about the same size.

In step 502, i.e. the washing and separating step, the sanitizing washing solution is injected at least once into the washing chamber 101 of the washing and separating container 1, said washing and separating container 1 being agitated to promote, by means of the emulsion forming means, the emulsification of the fluid components, in particular of the oil components with the sanitizing fluid substance, obtaining a stratification of the solid components on the liquid emulsion, said stratification of the solid components comprising the solid components of the cell debris, the cells and the one or more cell agglomerates; clean washing solution is injected through the inlet 102 and the emulsion of fluid components is discharged from the washing chamber 101 through the outlet 103 of the washing and separating vessel 1 and the removal effect of the density gradient of the emulsion of fluid components. This washing and separation step is repeated until the discharged fluid component is free of any impurities such as oil and/or blood.

In step 503, the solid component discharging step, the washing and separating vessel 1 is vertically positioned with the outlet 103 facing upward to allow the solid component to float on the washing solution, and the washing solution is injected under pressure through the inlet 102 of the washing and separating vessel 1 to allow the solid component to be discharged from the outlet 103.

Wherein the washing solution injecting step, the emulsion discharging step and the stirring step for producing the emulsion are all performed with the container 1 arranged in a vertical position, with the outlet 103 facing downwards, i.e. towards the ground.

Alternatively, the stirring step for producing the emulsion is carried out with the container 1 arranged horizontally with respect to the ground.

The above-described process allows obtaining transplantable adipose tissue/adipocytes that can be directly injected into a patient or stored under appropriate conditions, with a simple, rapid and inexpensive process.

The above disclosed devices, kits and methods forming the subject of the present invention can obviously be used not only for the preparation of adipose tissue to be transplanted, but also for the preparation of any type of tissue/cell mass that needs to have a high level of purity for use.

It should be understood by those skilled in the art that the above embodiments are only for illustrating the present invention and are not to be used as a limitation of the present invention, and that changes and modifications to the above embodiments are within the scope of the claims of the present invention as long as they are within the spirit and scope of the present invention.

Claims

1. A washing and separating vessel (1) for preparing adipose tissue, having a washing chamber (101) for washing fatty material, an inlet (102) and an outlet (103), the washing and separating vessel (1) being adapted to bring fatty material into the washing chamber (101) through the inlet (102) and to bring a fluid component of said material out of said washing chamber (101) through said outlet (103), characterized in that said washing chamber (101) comprises inside it a plurality of stirring assemblies (4) for mechanically forming an emulsion of the fluid component, each stirring assembly (4) comprising an elastic member (41) and at least one ball (42) attached to said elastic member (41); wherein one end of the elastic component (41) is fixedly connectedIn the processThe other end of the elastic component (41) is fixedly connected with the side wall of the washing and separating container (1) and the stirring component is spirally arranged on the side wall of the washing and separating container (1).

2. The washing and separating vessel (1) for preparing adipose tissue according to claim 1, wherein the elastic member (41) is one or more of a coil spring, a leaf spring or an elastic rod.

3. A washing and separating container (1) for preparing adipose tissue according to claim 2, wherein the length of said elastic means (41) at rest is greater than or equal to the radius of the container cross section.

4. A washing and separating vessel (1) for preparing adipose tissue according to any of the claims 1 to 3, wherein one of said spheres (42) is arranged on each of said elastic members (41), said sphere (42) being located at said one end of said elastic member (41).

5. Washing and separating vessel (1) for preparing adipose tissue according to any of the claims 1 to 3, wherein a plurality of said spheres (42) are provided on each of said elastic means (41).