CN209292325U - Adipose tissue preparation facilities - Google Patents
Adipose tissue preparation facilities Download PDFInfo
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- CN209292325U CN209292325U CN201821611517.4U CN201821611517U CN209292325U CN 209292325 U CN209292325 U CN 209292325U CN 201821611517 U CN201821611517 U CN 201821611517U CN 209292325 U CN209292325 U CN 209292325U
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- elastomeric element
- adipose tissue
- preparation facilities
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- 210000000577 adipose tissue Anatomy 0.000 title claims abstract description 31
- 238000002360 preparation method Methods 0.000 title claims abstract description 25
- 238000005406 washing Methods 0.000 claims abstract description 96
- 239000000463 material Substances 0.000 claims abstract description 39
- 239000000839 emulsion Substances 0.000 claims abstract description 21
- 239000012530 fluid Substances 0.000 claims abstract description 20
- 238000002156 mixing Methods 0.000 claims abstract description 18
- 238000000926 separation method Methods 0.000 claims description 64
- 230000003068 static effect Effects 0.000 claims description 2
- 230000002093 peripheral effect Effects 0.000 claims 1
- 230000033001 locomotion Effects 0.000 abstract description 6
- 239000011324 bead Substances 0.000 abstract description 5
- 206010021703 Indifference Diseases 0.000 abstract description 2
- 230000009514 concussion Effects 0.000 abstract description 2
- 238000011079 streamline operation Methods 0.000 abstract description 2
- 239000011435 rock Substances 0.000 abstract 1
- 239000007788 liquid Substances 0.000 description 18
- 239000003921 oil Substances 0.000 description 18
- 210000004027 cell Anatomy 0.000 description 17
- 210000001789 adipocyte Anatomy 0.000 description 16
- 238000000034 method Methods 0.000 description 15
- 239000007787 solid Substances 0.000 description 12
- 238000010586 diagram Methods 0.000 description 10
- 239000000243 solution Substances 0.000 description 9
- 238000003756 stirring Methods 0.000 description 9
- 210000001519 tissue Anatomy 0.000 description 9
- 210000004369 blood Anatomy 0.000 description 6
- 239000008280 blood Substances 0.000 description 6
- 238000004945 emulsification Methods 0.000 description 6
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- 230000000694 effects Effects 0.000 description 5
- JEIPFZHSYJVQDO-UHFFFAOYSA-N iron(III) oxide Inorganic materials O=[Fe]O[Fe]=O JEIPFZHSYJVQDO-UHFFFAOYSA-N 0.000 description 5
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Abstract
The utility model relates to a kind of adipose tissue preparation facilities, with the washing chamber for washing fat material, entrance and exit, the chamber is left for making fat material pass through outlet by the fluid components that entrance enters washing chamber and makes the material, mixing component including at least one emulsion for being used to mechanically form fluid components in the washing chamber, each mixing component include elastomeric element and at least one sphere for being fixed on elastomeric element.Since elastomeric element and sphere are connected, it slight concussion and rocks and can also cause the movement of bead by a relatively large margin in container, and the overturning of spring causes indifference eddy motion, to more effectively emulsion be promoted to be formed, thus can streamline operation, improve working efficiency.
Description
Technical field
The utility model relates to for preparing the fat for transplanting from the leaflet fat extracted by suction lipectomy
The device of tissue.
Background technique
Early in the seventies of eighties of last century, autologous fat transplantation become plastic surgery circle confirmation method after, just gradually by
It is applied to the every field of beauty and shaping.Autologous fat transplantation needs handle the fat of sucking, it is important that must be first
The fatty oil component generated in red blood cell and broken fat cell in the sucking fat of discharge.This is because red blood cell point
Normal tissue is destroyed at branch after solution, to destroy fat cell and reduce fatty transplanting success;And fat oil leaves
Acid free fatty acid can be decomposed and be formed to fat cell, also become the factor for destroying normal tissue and normal fat cells and
Fat transfer survival rate is reduced, and the formation of this free fatty acid can also cause and increase cell death, these necrotic tissues
It is easy the probability for increasing superinfection or culturing base as bacterium, and fat oil can stay in the wall of fat cell and thin
Intercellular interferes oxygen supply necessary to the existence of the fat cell of transplanting to cause fat cell downright bad as blood film.Because this
A little reasons generally need to remove fat oil before fat transfer, but are influenced by operating condition and equipment, are still difficult to accomplish
It is kept completely separate, still understands some and be mixed into transplant fat, the lower part in transplanting space is common when especially transplanting amount is more
The fat oil of aggregation will affect surgical effect in this way, and the symptom of appearance includes causing inflammation or destroying tissue, form big lump,
As lump etc. when touch.
In order to remove the red blood cell and fat oil that extract in fat, the method for most original is the fat utilization funnel that will be extracted
The container of apperance stands natural separation, but is difficult fractionation of fatty oil in this way and (is located on fat after fat oil separation
Layer), and elapsed time is relatively more.Another original processing method is to be squeezed to separate with hand after taking cloth to wrap in the fat of extraction
Impurity, while the dynamics compressed fat by squeezing, achieve the purpose that separating and filtering goes out fat oil, but same flower in this way
Take many times, and process is cumbersome, separating effect is related with operator, and prior this method has the hand of fat with people
And the risk of infection after air contact.
It is some using the container of syringe form as separator for safely and effectively separating red corpuscle and fat oil
The exploitation of technical research and relevant apparatus is reported successively.One kind is disclosed in Chinese invention patent application 200480002834.0
Closed loop fat transplantation system can see from the description of its specification, and the implant system is mainly for larger amount of fat transfer
Operation achievees the purpose that bacterium infection, but each portion of the system will not occur in liposuction, liquid separation and fat transfer process
Component relationship between part is relative complex, requires operation relatively high, is unfavorable for popularization and application, and the implant system is not examined
Consider the separation problem of fat oil.A kind of autologous fat transplantation rouge is disclosed in Chinese utility model patent 2006220113167.x
Fat cell collection screens syringe, inside and outside piston is arranged in syringe, it is complete to be formed in the tube negative pressure lacuna by fat suction machine
At liposuction, then need to take out the interior outer piston, be inserted into the original annotation emitter push-pull rod with flap valve through special restructuring, through from
The heart overflows grease by flap valve, then re-replaces the injection transplanting that syringe push-pull rod completes fat again.It can be seen that should
Centrifugation is relied solely on to the separation of fat oil in patented technology, the fat oil of spilling is simply positioned at fatty top, and is difficult to separate
Thoroughly, especially when only separating to a small amount of adipose tissue, accuracy is reduced, and to avoid being mixed into for fat oil, can only lose pure rouge
Fat tissue causes the loss of fat.
A kind of device for being used to prepare adipose tissue and side are disclosed in Chinese invention patent application 201180034851.2
Method, apparatus structure is as shown in Fig. 1, including at least one washing and separation vessel 1, at least one described washing and separation are held
Device 1 has the washing chamber 101 for washing liposuction materials, and the container 1 has entrance 102 and outlet 103, passes through for making
Suction lipectomy and the leaflet fat material that extracts by the entrance 102 enter the washing chamber 101 and for making the material
Fluid components in material leave the chamber 101 by the outlet 103, and the washing chamber 101 includes for passing through machine
Tool stirs and is formed the device of the emulsion of fluid components, and emulsion forms device by least one agitating element of ball etc.
104 are constituted, for increasing the emulsification of the liquid component when the washing and separation vessel 1 are subjected to mechanically or manually stirring.
It at least to include four steps as shown in attached drawing 2 when carrying out the preparation of fat cell using the device, first is that washing step,
Container 1 is maintained at vertical position in a manner of exporting 103 and face ground, by by the fluent material pressure of such as sterile saline
It is injected into the interior washing chamber 101 and is washed repeatedly, during obtaining no any oil, blood and any extraction
The high-purity cell mass (Fig. 2 a) of the solution used;Second is that emulsifying step, is oriented in horizontal position, i.e. less parallel for container
In ground, the container 1 is manually or mechanically stirred, to promote fluid components, specifically oily group with fluids for sterilization substance
Divide the emulsification (Fig. 2 b) with blood;Third is that emulsion removing step, is arranged in phase for container 1 to export 103 face-down modes
For being constituted consolidating on the emulsifying liquid liquid for being contained in the fat in washing chamber 101 to obtain in the vertical position on floor
The layering of body component, including the cell fragment, cell and one or more cell masses floated on the emulsion of fluid components
Solid component, by the emulsion of fluid components (that is, oil/liquid) by washing and the outlet 103 of separation vessel 1 from washing cavities
(Fig. 2 c) is discharged in room 101;Fourth is that solid component removing step, so that the outlet 103 of container 1 is vertically-oriented upward, so that solid group
(Fig. 2 d) point is swum on washing solution to which solid component to be discharged from washing and separation vessel.
The generation of emulsion is facilitated in foregoing invention patent application by the way that multiple beads are arranged in container as agitating device,
To improve the purity of fat cell, still, since bead has certain quality, bottom is sunk to when container is placed vertically
Portion is shaken in this case and is shaken, and the stirring action which is played is extremely limited, thus needs to cross in container
It is stirred operation again, i.e., emulsifying step mentioned above, which increase operation difficulties, moreover, increasing if it is manual operation
A possibility that having added pollution, then increases mechanical complexity if it is mechanically actuated, since it is desired that special be arranged so that container
The rotation drive device of horizontal position is rotated to from vertical position.Meanwhile in entire fat cell preparation process, need to carry out
At least three times container towards conversion operation, since container both ends are respectively connected with pipeline, repeatedly towards conversion operation the case where
Under inevitably the soundness of the connecting portion of pipeline and container is had an impact, thus a possibility that increasing pollution.
In addition, even if emulsifying step container is in horizontal positioned state, due to bead self gravity problem its in shaking/whipping process
Container can still be focused more on to move close to the position on ground, there are difference in whole container for stirring action, thus newborn
It is to be improved to change efficiency.
Thus, for above-mentioned technical problem of the existing technology, at present there are still can more easily carry out operation and
It is more effectively carried out the preparation facilities of the fat cell of emulsification and the demand of method.
Summary of the invention
The purpose of this utility model is to provide a kind of devices that can be avoided drawbacks described above, for the rouge by being pumped out
Fat material such as leaflet fat, rouge of the fat material preparation for transplanting i.e. comprising cell large crumb, cell and cell fragment
Fat tissue.
The other purpose of the utility model is the method for being related to preparing the adipose tissue for transplanting using described device,
The method and device allow to maintain biomaterial in completely enclosed system, and by relatively simple operation step and
The emulsification degree of relative efficiency prepares the higher adipose tissue of degree of purification.
The other purpose of the utility model is to provide the adipose tissue prepared by above-mentioned apparatus and method, especially rouge
Fat cell.It contains less blood, oil, so as to keep better surgical effect after the implantation.
Above-mentioned purpose by including that the device of at least one washing and separation vessel is realized, at least one described washing and
Separation vessel has the washing chamber of the fat material for washing suction, which has entrance and exit, for making fat
Material passes through outlet by the fluid components that entrance enters washing chamber and makes the material and leaves the chamber, the washing
Mixing component including at least one emulsion for being used to mechanically form fluid components in chamber, each mixing component include
Elastomeric element and at least one sphere being fixed on elastomeric element.
Preferably, the mixing component is multiple.
Optionally, elastomeric element is fixed on washing and separation vessel inner wall.
Optionally, elastomeric element is fixed on washing and separation vessel roof and bottom wall, and/or, elastomeric element is fixed on institute
State washing and separation vessel circumferential side wall.
Preferably, the mixing component is uniformly distributed in the circumferential around the longitudinal axis of the washing and separation vessel.
Optionally, elastomeric element one end is connected the sphere, and the other end is in free shape or is equally fixed with sphere.
Preferably, elastomeric element is helical spring, leaf spring and/or elastic rod.
Preferably, length of elastomeric element when static is more than or equal to the radius of container cross section.
Optionally, a sphere is set on each elastomeric element, and sphere is located at elastomeric element end.
Optionally, multiple spheres are set on each elastomeric element.
Preferably, multiple spheres are symmetrically laid along elastomeric element longitudinal axis.
Preferably, it is provided with size reducing apparatus in the washing chamber, for fat material to be processed to be cut into
Smaller size.
Preferably, size reducing apparatus includes that at least one size reduces net.
Preferably, the size reducing apparatus includes that first size reduces net and the second size and reduces net, be located at into
At mouthful and exit.
Preferably, the size reducing apparatus also include third size reduce net, be laid in first size reduce net and
Second size reduces between net.
Preferably, the mesh that the size reduces net is gradually reduced by entrance to outlet.
The purpose of this utility model also realizes that the external member includes at least one by being used to prepare the external member of adipose tissue
Be used to prepare adipose tissue washing and separation vessel, for from patient's suction of fat material syringe, for collecting/injecting
Syringe, wash liquid container, the container for collecting obsolete material of the biomaterial of processing, and for connecting above-mentioned portion
The pipeline of part.
The purpose of this utility model also realizes that the device includes washing and divides by being used to prepare the device of adipose tissue
From container and holding meanss, the holding meanss provide the washing and divide for keeping the washing and separation vessel
From rotation of the container in the plane perpendicular to ground.
Preferably, the holding meanss also provide it is described washing and separation vessel in the plane perpendicular to ground along upper
Lower and left and right directions vibration driving.
The utility model has the advantage that compared with the existing technology
1. since elastomeric element and sphere are connected, even if slight concussion and being rocked then when container is in a vertical state
Also it can cause the movement of bead by a relatively large margin in container, and the overturning of spring causes indifference eddy motion, thus more effectively
Ground promotes emulsion to be formed.Elastomeric element can be set to multiple, be evenly distributed on horizontal and vertical, this makes without vertical
Moving up container can be realized more sufficient stirring, improve work efficiency.
2, since stirring action is mainly completed by sphere, impact is generated for the tissue in container and is rubbed main next
Source is sphere, it is thus possible to which the integrality for farthest guaranteeing fat cell reduces the breakage rate of fat cell.
3, sufficient stirring can be realized in the simple movement in view of container, it is thus possible to which on the one hand streamline operation drops
On the other hand contaminated risk when low manual operation can also reduce complexity mechanical under Mechanical Driven operational circumstances.
4, it completes to stir without horizontal container due to the presence of elastomeric element, thus allows in container longitudinal middle part, example
As added one or more sizes reduction nets close to entrance namely positioned at the position of agitating element upstream, so as to guarantee wait locate
The size for managing material reduces degree and effect, further increases working efficiency.
Detailed description of the invention
It, below will be to required in embodiment or description of the prior art in order to illustrate more clearly of the technical solution of the application
The attached drawing used is briefly described, it should be apparent that, the drawings in the following description are only some examples of the present application, right
For those of ordinary skill in the art, without creative efforts, it can also be obtained according to these attached drawings
Its attached drawing.
Fig. 1 shows the washing of the preparation facilities of adipose tissue in the prior art and the side view of separation vessel.
Fig. 2 shows the operation streams that adipose tissue is prepared based on the preparation facilities comprising washing shown in FIG. 1 and separation vessel
Journey schematic diagram.
Fig. 3 shows the washing according to the first embodiment of the utility model and the vertical section schematic diagram of separation vessel.
Fig. 4 shows the washing according to the second embodiment of the utility model and the vertical section schematic diagram of separation vessel.
Fig. 5 shows the washing according to the third embodiment of the utility model and the vertical section schematic diagram of separation vessel.
Fig. 6 shows the washing according to the 4th embodiment of the utility model and the vertical section schematic diagram of separation vessel.
Fig. 7 shows the washing according to the 5th embodiment of the utility model and the vertical section schematic diagram of separation vessel.
Fig. 8 shows the fatty preparation facilities external member comprising washing and separation vessel according to the embodiments of the present invention
Schematic diagram.
Fig. 9 is the method flow diagram that adipose tissue is prepared using the fatty preparation facilities of the embodiments of the present invention.
Specific embodiment
Below in conjunction with Figure of description, the technical scheme in the embodiment of the application is clearly and completely described, shows
So, described embodiments are only a part of embodiments of the present application, instead of all the embodiments.Based on the reality in the application
Apply example, those of ordinary skill in the art's every other embodiment obtained without creative labor, all
Belong to the range of the application protection.
<washing and separation vessel>
<first embodiment>
Fig. 3 shows the washing according to the first embodiment of the utility model and the vertical section schematic diagram of separation vessel.It washes
The washing chamber 101 with separation vessel 1 with the material for washing suction is washed, the container 1 has entrance 102 and outlet
103, for making the fat material that gets of suction enter washing chamber 101 and sequentially in time by entrance 102, i.e., first
Fluid components and the then sequence of solid component leave the chamber 101 by outlet 103, and the washing chamber 101 includes using
In mechanically forming, fluid components, specifically the oil, blood and/or the other sterilised liqs that obtain from the fat cell of rupture are molten
The device of the emulsion of liquid.
The emulsion forms device and is made of at least one mixing component 4, at least one described mixing component 4 includes bullet
Property the component 41 and sphere 42 that is fixed on elastomeric element 41, specifically, elastomeric element 41 can be helical spring.Optionally,
Elastomeric element 41 is affixed to the top and bottom of washing and separation vessel 1.Sphere 42 is fixed on elastomeric element 41, for making
Increase the emulsion of liquid component when washing and separation vessel 1 are subjected to shaking, rocking.In the device of the utility model, container 1
Simplified manual stirring be capable of providing the separation of liquid phase and solid phase, the liquid phase includes fluid emulsion, solid phase packet obtained
Include cell, cell fragment, cell mass.
Optionally, the quantity of mixing component 4 is more than or equal to 4.
Optionally, mixing component 4 is uniformly distributed in container 1.For example, symmetrical in the center line of short transverse with container 1
It is distributed in the bottom wall and roof of container, and/or, it is circumferentially distributed centered on the longitudinal axis of container 1.
Optionally, it is provided with size reducing apparatus in washing and separation vessel 1, which includes several rulers
Very little reduction net.First size at least, which is provided with, at the entrance 102 of washing and separation vessel 1 reduces net 301.Material to be processed
Net 301 is reduced by the size before entering the washing chamber 101 of washing and separation vessel 1, it is smaller to cut the material into
Size.Optionally, the second size is also equipped at washing and the outlet 103 of separation vessel 1 reduce net 302.
Preferably, from entrance 102 to outlet 103, the mesh size that size reduces net successively decreases.
Size reduces net 3 and is made of at least one set of parallel or intersection sheet material or cutting line.Preferably, it the sheet material or cuts
Secant is made of pasteurization material such as metal.
The mesh that the size reduces net 34 can have various shape, such as, but not limited to: triangle, rectangle, water chestnut
Shape, hexagon, octagon, etc..
<second embodiment>
According to washing and the separation vessel of the second embodiment of the utility model as shown in figure 4, its structure is real with first
It is similar to apply mode, the difference is that, the elastomeric element 41 of helical spring form is affixed to the side wall of washing and separation vessel 1.
Optionally, mixing component is alternately laid in the opposite side of 1 inner wall of washing and separation vessel.
Optionally, helically form is laid on the side wall of washing and separation vessel 1 mixing component.
In this embodiment, optionally, reduce net 301 in first size and the second size reduces between net 302, compared to
103 are exported closer to the position of entrance 102, third size is set and reduces net 303.
Material to be processed reduces net by two-stage size before entering the washing chamber 101 of washing and separation vessel 1
301,303, so as to cut the material into smaller size.
<third embodiment>
According to washing and the separation vessel of the third embodiment of the utility model as shown in figure 5, its structure is real with first
It is similar (size reduces net omit is not shown) to apply mode, the difference is that, the elastomeric element 41 of helical spring form not with wash
It washs and is connected with separation vessel 1, but there is free-end.In this embodiment, elastomeric element 41 provide with the inner wall of container 1 it
Between sufficient bounce so that either container 1 be in be vertically oriented to still horizontally toward when, the shaking of container 1 can
Cause the active movement of sphere 42, to effectively improve emulsifying effectiveness.
Optionally, 41 both ends of elastomeric element of helical spring form are provided with sphere 42.
In this embodiment, optionally, reducing appointing between net 301 and the second size reduction net 302 in first size
One position adds 1-3 size and reduces net, thus further increases size and reduces effect, ensures the segregation ratio of adipose tissue.
<the 4th embodiment>
According to washing and the separation vessel of the 4th embodiment of the utility model as shown in fig. 6, its structure is real with first
It is similar (size reduces net omission and is not shown) to apply mode, the difference is that, the elastomeric element 41 that mixing component 4 is included is piece
Spring, the elastomeric element 41 of leaf spring form are affixed to container inner wall, and sphere 42 is affixed to elastomeric element 41.
Optionally, the elastomeric element 41 of leaf spring form is affixed to the upper and lower ends of washing and separation vessel 1 or is connected
Onto its inner wall.
In this embodiment, be used as the leaf spring of elastomeric element 41 direction can be identical, for example, leaf spring area compared with
Big one side can also be different both facing to some direction, for example, the biggish one side of leaf spring area is both facing to chamber wall, or
It is directed towards random arrangement.
Optionally, the length of leaf spring can be identical, can also be different from each other, for example, being in the alternate form of length, either
The form spirally lengthened.
<the 5th embodiment>
According to washing and the separation vessel of the 5th embodiment of the utility model as shown in fig. 7, its structure is real with first
It is similar (size reduces net omission and is not shown) to apply mode, the difference is that, mixing component 4 includes elastomeric element 41 and sphere
42, wherein more than one sphere 42 is fixed on each elastomeric element 41.
Optionally, elastomeric element 42 is helical spring, is affixed to 1 inner wall of washing and separation vessel, or be free on
In washing chamber.
Optionally, more than one sphere 42 is affixed to the same side or not ipsilateral of same elastomeric element 42.
Optionally, it is connected between sphere 42 and elastomeric element 42 by short silk.
<adipose tissue preparation facilities and external member>
Fig. 8 shows the fatty preparation facilities external member comprising washing and separation vessel according to the embodiments of the present invention
Schematic diagram.The external member allows the transfer of fat material that can occur in closed system, and donor is come from the closed system
Fat material and final obtained adipose tissue do not contact external environment completely.
The external member includes the washing described according to above embodiment and separation vessel 1, for from patient's sucked material
Syringe 7, for collect/syringe 12 of the biomaterial of injection treatment, wash liquid container 10 is (for example, accommodate salt water
Sack), the container 11 for collecting obsolete material, they by sterilization pipe, changeover valve, luer connector connection or can connect
To together.
Specifically, washing and 1 entrance 102 of separation vessel and/or outlet 103 can be connected to three-way valve 9, such as existing skill
Known Luer lock syringe 7 is connected to the washing equipped with three-way valve 9 and the entrance of separation vessel 1 by pipe or conduit 8 in art
102, which carries out the suction of fat material via the intubation for being equipped with one or more holes on the surface thereof.
The liquid that such as physiological saline is accommodated in wash liquid sack 10, be used for carry out fat material washing and
The air in container 1 is excluded before separation, offer pressure forces fat material to enter and leave chamber 101 and to fat material
It is washed repeatedly.
Syringe 12 is connected to outlet 103, for collecting the prepared biomaterial mixed with liquid, is allowed to sink
It forms sediment to obtain the separation of solid component and remaining liquid component.The material of storage within the container 12 can be designed to final
Reduce in container 2 before saving and/or injecting again in washing and separation vessel 1 and/or size and is handled again.
For collecting the container such as sack 11 of obsolete material by 9 connection outlet 103 of three-way valve, by emulsified liquid collection its
In.
Washing and separation vessel 1 can be kept by a holding meanss (not shown), and fortune needed for providing the container 1
Dynamic driving, for example, rotation of the container 1 in the plane perpendicular to ground is capable of providing, to realize by exporting 103 towards ground
Converted to entrance 102 towards the inversion on ground, meanwhile, can also provide upper and lower in the plane perpendicular to ground of container 1 and/
Or the vibration driving of left and right.Optionally, holding meanss can also provide container 1 around the rotation of the axis perpendicular to ground.
<adipose tissue preparation flow>
Fig. 9 shows the method flow that adipose tissue is prepared using the fatty preparation facilities of the embodiments of the present invention
Figure.
Specifically, in step 501, i.e. size reduction step, by gravity/injection via the liquid in wash liquid container 10
Body provides pressure, so that aspirating resulting leaflet fat passes through size reducing apparatus at least once, to separate cell mass, makes
The cell mass is obtained as predetermined size greatly or less than predetermined size, and the cell mass is had on an average
Roughly the same size.
In step 502, i.e. washing and separating step, disinfecting cleaning solution is injected into the washing of washing and separation vessel 1
In chamber 101 at least once, the washing and separation vessel 1 are stirred, promotes fluid components to form device by emulsion
Emulsification specifically promotes the emulsification of oil ingredient and fluids for sterilization substance, obtains layering of the solid component on emulsifying liquid liquid,
The layering of the solid component includes the solid component of cell fragment, cell and one or more cell masses;By it is described enter
Mouth 102 injects clean washing solution, and passes through the outlet 103 and fluid group of the washing and separation vessel 1
The removing of the density gradient of the emulsion divided acts on and the emulsion of the fluid components is discharged from the washing chamber 101.
The washing and separating step are repeated, until the fluid components of discharge are without such as any impurity of oil and/or blood.
In step 503, i.e., step is discharged in solid component, the outlet 103 upward in the case where by the washing and point
It vertically positions from container 1, to allow the solid component to swim on the washing solution, is held by the washing and separation
The entrance 102 of device 1 under pressure injects washing solution, to allow the solid component to be discharged from outlet 103.
Wherein, the washing solution injecting step, the emulsion are discharged step and for generating the emulsion
Whipping step is to be disposed in vertical position in the container 1, and the outlet 103 is downward, i.e., the feelings towards ground
It is carried out under condition.
Optionally, the whipping step for generating the emulsion is in the container 1 by relative to surface water level land cloth
It is carried out in the case where setting.
Allow to obtain transplantable adipose tissue/fat by simple, rapid and low price processing by above-mentioned processing thin
Born of the same parents can directly inject to patient, or be stored under the suitable conditions.
Device disclosed above, external member and the method for forming the theme of the utility model can significantly be applied not only to prepare
Adipose tissue to be transplanted, but also be used to prepare need to have high level of purity for use any kind of tissue/it is thin
Born of the same parents/cell mass.
Those of ordinary skill in the art it should be appreciated that more than embodiment be intended merely to illustrate that this is practical new
Type, and be not used as the restriction to the utility model, as long as in the spirit of the utility model, to the above
Variation, the modification of embodiment will all be fallen in the scope of the claims of the utility model.
Claims (10)
1. a kind of adipose tissue preparation facilities, have washing chamber (101) for washing fat material, entrance (102) and
It exports (103), for making fat material pass through the fluid group that entrance (102) enter washing chamber (101) and make the material
Divide and leave the washing chamber (101) across the outlet (103), which is characterized in that includes extremely in the washing chamber (101)
The mixing component (4) of few one emulsion for mechanically forming fluid components, each mixing component (4) include elastic portion
Part (41) and at least one sphere (42) being fixed on elastomeric element (41).
2. adipose tissue preparation facilities as described in claim 1, wherein the mixing component is multiple.
3. adipose tissue preparation facilities as described in claim 1, wherein the elastomeric element (41) be fixed on it is described washing and
Separation vessel (1) inner wall.
4. adipose tissue preparation facilities as claimed in claim 3, wherein the elastomeric element (41) be fixed on it is described washing and
Separation vessel (1) roof and bottom wall, and/or, the elastomeric element (41) is fixed on the washing and separation vessel (1) peripheral side
Wall.
5. adipose tissue preparation facilities as claimed in claim 4, wherein the mixing component (4) surrounds the washing and separation
The longitudinal axis of container (1) is uniformly distributed in the circumferential.
6. adipose tissue preparation facilities as described in claim 1, wherein described elastomeric element (41) one end is connected the sphere
(42), the other end is in free shape or is equally fixed with sphere (42).
7. such as adipose tissue preparation facilities of any of claims 1-6, wherein the elastomeric element (41) is spiral
Spring, leaf spring and/or elastic rod.
8. adipose tissue preparation facilities as claimed in claim 7, wherein length of the elastomeric element (41) when static is big
In the radius for being equal to container cross section.
9. adipose tissue preparation facilities according to any one of claims 1 to 5, wherein on each elastomeric element (41)
One sphere (42) is set, and the sphere (42) is located at the elastomeric element (41) end.
10. such as adipose tissue preparation facilities of any of claims 1-6, wherein each elastomeric element (41)
The upper multiple spheres (42) of setting.
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CN114887348A (en) * | 2022-06-10 | 2022-08-12 | 中国海洋大学 | Edible salt crystallization device |
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CN114887348A (en) * | 2022-06-10 | 2022-08-12 | 中国海洋大学 | Edible salt crystallization device |
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