CN109223897A - A kind of tobacco active component, extracting method and its application - Google Patents
A kind of tobacco active component, extracting method and its application Download PDFInfo
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- A61K2236/30—Extraction of the material
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Abstract
The present invention provides a kind of tobacco active component, extracting method and its applications, belong to tobacco product processing extractive technique field.The present invention is by pre-processing tobacco material, and for pretreated tobacco material successively carry out room temperature extraction, high temperature extract, methanol extraction operate three times, and use highly polar solvent purification water, methanol as Extraction solvent, by using different solvents various extracting conditions, it can guarantee to realize the tobacco active component in tobacco material and sufficiently extract, improve the extraction content of tobacco active component.Experiment finds that the purpose component has antibacterial, anti-tumor function, and is hypotoxicity product.Therefore there is antibacterial, anticancer drug potentiality to be exploited, theoretical foundation is provided for new drug development.
Description
Technical field
The present invention relates to tobacco products to process extractive technique field, and in particular to a kind of tobacco active component, extracting method
And its application.
Background technique
Tobacco is also referred to as mesona, is Chinese medicine traditional simply, its inorganic constituents includes water, mineral element；Organic principle
It mainly include carbohydrate, alkaloid, heterocyclic, pigment, phenols, terpene, organic acid, lipid, alcohols, aldoketones etc..Wherein,
Organic principle has antibacterial, antiviral and antitumor, antioxidant activity, removes the multiple functions such as interior free yl in tobacco.
Currently, generally using means of supercritical extraction, Soxhlet extraction, ultrasonic wave to realize the extraction to tobacco active component
Extracting mode.Using dehydrated alcohol, n-hexane, acetone, ethyl acetate, petroleum ether, methylene chloride as Extraction solvent.It is wherein low
Polar solvent such as petroleum ether, n-hexane, middle polar solvent acetone, methylene chloride, these solvents are mostly volatile, inflammable, toxic
Solvent requires height to the equipment of industrial product, personnel etc., and the content of obtained tobacco active component is few, this is just greatly limited
The further research and application of tobacco active component.
Summary of the invention
In view of the above-mentioned problems, the present invention provides a kind of tobacco active component, extracting method and its application, it is living to improve tobacco
The extracted amount of property component.
In a first aspect, the present invention provides a kind of extracting methods of tobacco active component, method includes the following steps:
(1) it pre-processes: drying and processing being carried out to tobacco material, and the tobacco material after drying and processing is subjected to Ultramicro-powder
Broken processing obtains tobacco Ultramicro-powder；
(2) normal-temperature water mentions: Extraction solvent being added in tobacco Ultramicro-powder, extracts operation at normal temperature；It is grasped to extracting
Tobacco Ultramicro-powder after work carries out centrifugal treating, and collects the sediment after centrifugal treating；Extraction solvent in step (2) is pure
Change water；
(3) high-temperature water mentions: Extraction solvent is added in the sediment collected in step (2), in 90-100 DEG C of extraction temperature
Degree is lower to carry out concentration extraction operation；Cooling processing, centrifugal treating are carried out to the concentrated extracting solution obtained after concentration extraction operation, and
Sediment after collecting centrifugal treating；Extraction solvent in step (3) is purified water；
(4) methanol extracts: Extraction solvent is added in the sediment obtained in step (3), under 0-4 DEG C of Extracting temperature
Soakage extraction operation is carried out, centrifugal treating is carried out to Soakage extraction object, and collect the supernatant after centrifugal treating, by the supernatant of collection
It carries out rotary evaporation and drying and processing is concentrated, obtain tobacco active component；The first that Extraction solvent in step (4) is 85-100%
Alcohol.
Preferably, the drying and processing in step (1) is to dry tobacco material placement in an oven, so that at drying
The water content of tobacco material after reason is less than 5%, and drying temperature is 50-60 DEG C, baking time 4-8h；
Preferably, the ultramicro crushing treatment in step (1) is that the tobacco material after drying and processing is used 50-80 mesh
The pulverizer of net carries out pulverization process, and to the tobacco material after pulverization process, is carried out using the pulverizer of 200-400 mesh screen
Pulverization process obtains the tobacco Ultramicro-powder.
Preferably, the middle quality that Extraction solvent is added of step (2) is 2-4 times of the quality of the tobacco Ultramicro-powder；
Preferably, the extraction operation in step (2), which refers to, is placed in the tobacco Ultramicro-powder and Extraction solvent in extractor,
Extraction time is 0.5-3h；
Preferably, the centrifugal treating in step (2) refers to: centrifuging temperature is 8-12 DEG C, centrifugation time 0.5-1.5h, is turned
Speed is 3000-6000rpm/min.
Preferably, the middle quality that Extraction solvent is added of step (3) is 1.2-2.4 times of the quality of sediment；
Preferably, the extraction time of the concentration extraction operation in step (3) is 0.5-1.5h；
Preferably, the centrifugal treating in step (3) refer to will cooling treated that the concentrated extracting solution is placed in centrifugal bottle
In, centrifuging temperature is 8-12 DEG C, centrifugation time 0.5-1.5h, revolving speed 3000-6000rpm/min.
Preferably, the middle quality that Extraction solvent is added of step (4) is 1.5-2.5 times of the quality of sediment；
Preferably, the Soakage extraction operation in step (4) refers to, extraction time 12-24h primary every 0.5h stirring；
Preferably, the centrifugal treating in step (4), which refers to, is placed in the Soakage extraction object in centrifugal bottle, centrifuging temperature
Are as follows: 3-5 DEG C, centrifugation time 0.5-1.5h, revolving speed 3000-6000rpm/min；
Preferably, the temperature of the concentration drying and processing in step (4) is 60-70 DEG C.
Second aspect, the present invention provides a kind of tobacco active groups extracted according to any of the above-described extracting method
Point.
The third aspect, the present invention provides a kind of according to the tobacco active component in preparation antibacterial, in anticancer drug
Using.
The present invention provides a kind of tobacco active component, extracting method and its application, by being pre-processed to tobacco material,
And for pretreated tobacco material successively carry out room temperature extraction, high temperature extract, methanol extraction operate three times, and use high pole
Property solvent purification water, methanol are as Extraction solvent, by using different solvents various extracting conditions, it is ensured that by tobacco
Tobacco active component in raw material is realized and is sufficiently extracted, and the extraction content of tobacco active component is improved.
The above description is only an overview of the technical scheme of the present invention, in order to better understand the technical means of the present invention,
And can be implemented in accordance with the contents of the specification, below with presently preferred embodiments of the present invention.
Detailed description of the invention
Fig. 1 is the flow chart of tobacco active component extracting method；
Fig. 2 is that tobacco active component inhibits fungi-Cordyceps Militaris growth schematic diagram；
Fig. 3 is that tobacco active component inhibits bacterium-Escherichia coli Growth schematic diagram；
Fig. 4 is tobacco active component to human lung cancer's A549 cell growth inhibition schematic diagram；
Fig. 5 is tobacco active component to human liver cancer Hep-G2 cell growth inhibition schematic diagram；
The above description is only an overview of the technical scheme of the present invention, in order to better understand the technical means of the present invention,
And can be implemented in accordance with the contents of the specification, below with presently preferred embodiments of the present invention.
Specific embodiment
The present invention is further described in detail with reference to embodiments.It is noted that detailed description below is all to illustrate
Property, it is intended to further instruction is provided to the present invention.Unless otherwise indicated, all scientific and technical terms that the present invention uses
With with the normally understood identical meanings of the technical field of the invention personnel.
The present invention provides a kind of extracting method of tobacco active component, which please refers to process shown in FIG. 1
Figure, the extracting method may comprise steps of:
(1) it pre-processes: drying and processing being carried out to tobacco material, and the tobacco material after drying and processing is subjected to Ultramicro-powder
Broken processing obtains tobacco Ultramicro-powder；
(2) normal-temperature water mentions: Extraction solvent being added in tobacco Ultramicro-powder, extracts operation under normal temperature conditions；To mentioning
Tobacco Ultramicro-powder after extract operation carries out centrifugal treating, and collects the sediment after centrifugal treating；Extraction solvent in step (2)
For purified water；
(3) high-temperature water mentions: Extraction solvent is added in the sediment collected in step (2), in 90-100 DEG C of extraction temperature
Degree is lower to carry out concentration extraction operation；Cooling processing, centrifugal treating are carried out to the concentrated extracting solution obtained after concentration extraction operation, and
Sediment after collecting centrifugal treating；Extraction solvent in step (3) is purified water；
(4) methanol extracts: Extraction solvent is added in the sediment obtained in step (3), under 0-4 DEG C of Extracting temperature
Extraction extraction operation is carried out, centrifugal treating is carried out to Soakage extraction object, and collect the supernatant after centrifugal treating, by the supernatant of collection
It carries out rotary evaporation and drying and processing is concentrated, obtain tobacco active component；The first that Extraction solvent in step (4) is 85-100%
Alcohol.
Extracting method of the invention is further described with specific embodiment separately below.
Embodiment 1
The embodiment of the present invention 1 provides a kind of extracting method of tobacco active component, comprising the following steps:
(1) it pre-processes.
30Kg tobacco material is weighed, placement is dried in an oven, and 50 DEG C of drying temperature, baking time 4h is dried to cigarette
Careless water content is taken out less than 5%；Tobacco material after taking drying and processing after being crushed using the pulverizer of 50 mesh screens, and is put into
Ultramicro crushing treatment is carried out in the pulverizer of 200 mesh screens, obtains tobacco Ultramicro-powder.
(2) normal-temperature water mentions.
It weighs 30Kg tobacco Ultramicro-powder to be added in 200L extractor, 100Kg purified water is added, stirring at normal temperature is extracted
0.5h；Stirring is transferred in centrifugal bottle after extracting, 8 DEG C of centrifugations 0.5h, revolving speed 3000rpm/min；Collect centrifugal treating
Sediment afterwards.
(3) high-temperature water mentions.
The sediment 25Kg in step (2) is weighed, is added in 100L concentration extraction tank；60Kg purified water is added, opens
Concentration extraction tank heater switch；After being heated to 90 DEG C, in 90 DEG C of at a temperature of concentration extraction 0.5h；Into concentration extraction tank collet
It is passed through recirculated water, to carry out cooling processing to concentrated extracting solution, it is made to be cooled to room temperature；Concentrated extracting solution is transferred to centrifugal bottle
Middle carry out centrifugal treating, 8 DEG C of centrifugations 0.5h, revolving speed 3000rpm/min；And collect the sediment after centrifugal treating.
(4) methanol extracts.
Sediment obtained in step (3) is added in stainless steel barrel, every barrel of 10Kg；The first of 15Kg is added in every barrel
Alcohol, the mass fraction of methanol are 92%, and Soakage extraction operates under 0 DEG C of Extracting temperature；Wherein, extraction time 12h, every
0.5h stirs substance in bucket primary；Soakage extraction object is placed in centrifugal bottle, 3 DEG C of centrifugations 1.5h, revolving speed 3000rpm/
min；The supernatant of collection is transferred to 60 DEG C of concentrations in 50L revolving, after the completion of concentration by the supernatant after collecting centrifugal treating
It is transferred in baking pan, 60 DEG C are baked into medicinal extract, and the active component of tobacco is obtained.
Embodiment 2
The embodiment of the present invention 2 provides a kind of extracting method of tobacco active component, comprising the following steps:
(1) it pre-processes.
40Kg tobacco material is weighed, placement is dried in an oven, and 55 DEG C of drying temperature, baking time 6h is dried to cigarette
Careless water content is taken out less than 5%；Tobacco material after taking drying and processing after being crushed using the pulverizer of 60 mesh screens, and is put into
Ultramicro crushing treatment is carried out in the pulverizer of 300 mesh screens, obtains tobacco Ultramicro-powder.
(2) normal-temperature water mentions.
It weighs 40Kg tobacco Ultramicro-powder to be added in 200L extractor, 120Kg purified water is added, stirring at normal temperature is extracted
1.5h；Stirring is transferred in centrifugal bottle after extracting, 10 DEG C of centrifugations 1h, revolving speed 5000rpm/min；After collecting centrifugal treating
Sediment.
(3) high-temperature water mentions.
The sediment 30Kg in step (2) is weighed, is added in 100L concentration extraction tank；60Kg purified water is added, opens
Concentration extraction tank heater switch；After being heated to 100 DEG C, in 100 DEG C of at a temperature of concentration extraction 1h；Into concentration extraction tank collet
It is passed through recirculated water, to carry out cooling processing to concentrated extracting solution, it is made to be cooled to room temperature；Concentrated extracting solution is transferred to centrifugal bottle
Middle carry out centrifugal treating, 10 DEG C of centrifugations 1.5h, revolving speed 5000rpm/min；And collect the sediment after centrifugal treating.
(4) methanol extracts.
Sediment obtained in step (3) is added in stainless steel barrel, every barrel of 10Kg；The first of 25Kg is added in every barrel
Alcohol, the mass fraction of methanol are 85%, and Soakage extraction operates under 4 DEG C of Extracting temperature；Wherein, extraction time 18h, every
0.5h stirs substance in bucket primary；Soakage extraction object is placed in centrifugal bottle, 4 DEG C of centrifugations 1h, revolving speed 5000rpm/min；
The supernatant of collection is transferred to 65 DEG C of concentrations in 50L revolving, shifted after the completion of concentration by the supernatant after collecting centrifugal treating
Into baking pan, 65 DEG C are baked into medicinal extract, obtain the active component of tobacco.
Embodiment 3
The embodiment of the present invention 3 provides a kind of extracting method of tobacco active component, comprising the following steps:
(1) it pre-processes.
50Kg tobacco material is weighed, placement is dried in an oven, and 60 DEG C of drying temperature, baking time 8h is dried to cigarette
Careless water content is taken out less than 5%；Tobacco material after taking drying and processing after being crushed using the pulverizer of 80 mesh screens, and is put into
Ultramicro crushing treatment is carried out in the pulverizer of 400 mesh screens, obtains tobacco Ultramicro-powder.
(2) normal-temperature water mentions.
It weighs 50Kg tobacco Ultramicro-powder to be added in 200L extractor, 120Kg purified water is added, stirring at normal temperature is extracted
0.5h；Stirring is transferred in centrifugal bottle after extracting, 12 DEG C of centrifugations 1.5h, revolving speed 6000rpm/min；Collect centrifugal treating
Sediment afterwards.
(3) high-temperature water mentions.
The sediment 45Kg in step (2) is weighed, is added in 100L concentration extraction tank；60Kg purified water is added, opens
Concentration extraction tank heater switch；After being heated to 95 DEG C, in 95 DEG C of at a temperature of concentration extraction 1.5h；Into concentration extraction tank collet
It is passed through recirculated water, to carry out cooling processing to concentrated extracting solution, it is made to be cooled to room temperature；Concentrated extracting solution is transferred to centrifugal bottle
Middle carry out centrifugal treating, 12 DEG C of centrifugations 1h, revolving speed 6000rpm/min；And collect the sediment after centrifugal treating.
(4) methanol extracts.
Sediment obtained in step (3) is added in stainless steel barrel, every barrel of 10Kg；The first of 25Kg is added in every barrel
Alcohol, the mass fraction of methanol are 100%, and Soakage extraction operates under 0 DEG C of Extracting temperature；Wherein, extraction time is for 24 hours, often
Substance in bucket is stirred every 0.5h primary；Soakage extraction object is placed in centrifugal bottle, 5 DEG C of centrifugations 1.5h, revolving speed 6000rpm/
min；The supernatant of collection is transferred to 70 DEG C of concentrations in 50L revolving, after the completion of concentration by the supernatant after collecting centrifugal treating
It is transferred in baking pan, 70 DEG C are baked into medicinal extract, and the active component of tobacco is obtained.
Embodiment 4: tobacco active component inhibits the experiment of fungi-Cordyceps Militaris
(1) solid medium: yeast extract 0.2g, peptone 0.5g, glucose 0.2g, dipotassium hydrogen phosphate is configured
In 100ml, high pressure sterilization falls flat when temperature is suitable in superclean bench for 0.1g, magnesium sulfate 0.05g, agar 1.5g constant volume
Plate uses after solidification.
(2) Cordyceps Militaris is inoculated with: Cordyceps Militaris being inoculated into solid medium culture dish, is coated with uniformly, is covered with spreading rod
Culture dish.
(3) agar punches: making a call to three holes with 1ml pipette tips on the culture dish for be coated with bacterium solution.
(4) it adds tobacco active component: having beaten hole and chosen the partial medium that pipette tips in hole are not taken out of with sterile medicine Chinese herbaceous peony,
Tobacco active component is diluted with pure water, concentration 0.2g/ml pipettes 200 μ L dilutions to the three of bacterium solution culture dish with liquid-transfering gun
In a hole, culture dish carries out Cordyceps Militaris label and experiment date.
(5) it cultivates: culture dish being put into incubator 27 DEG C, is cultivated 48-96 hours.
(6) experimental result is as shown in Figure 2: using the culture dish seen after piece machine checks culture, not giving birth to around hole
Strain is grown, therefore shows that tobacco active component is inhibited to Cordyceps Militaris.
Embodiment 5: tobacco active component inhibits the experiment of bacterium-Escherichia coli
(1) solid medium is arranged: yeast extract 0.2g, peptone 0.5g, glucose 0.2g, agar 1.5g constant volume exist
100ml, high pressure sterilization, when temperature is suitable, the inverted plate in superclean bench uses after solidification.
(2) Escherichia coli are inoculated with: Escherichia coli are inoculated into solid medium culture dish, are coated with uniformly with spreading rod,
Cover culture dish.
(3) agar punches: making a call to three holes with 1ml pipette tips on the culture dish for be coated with bacterium solution.
(4) it adds tobacco active component: having beaten hole and chosen the partial medium that pipette tips in hole are not taken out of with sterile medicine Chinese herbaceous peony,
Tobacco active component is diluted with pure water, concentration 0.2g/ml pipettes 200 μ L dilutions to the three of bacterium solution culture dish with liquid-transfering gun
In a hole, culture dish carries out Escherichia coli label and experiment date.
(5) it cultivates: culture dish being put into incubator 37 DEG C, is cultivated 48-96 hours.
(6) experimental result is as shown in Figure 3: seeing the culture dish after piece machine checks culture, does not grow around hole
Strain, therefore show that tobacco active component is inhibited to Escherichia coli.
Embodiment 6: the toxicological experiment of tobacco active component
Animal: healthy 54 half male and half female weight (30g or so) of mouse.
Drug: the tobacco active component that embodiment 1 obtains
Experimental method: 50 healthy mices are divided into 5 groups, every group 10, half male and half female is divided into high dose group, higher dose
Amount group, middle dose group, low dose group, relatively low-dose group, low dose group.Taking 4 healthy mices again is one group of half male and half female conduct
Blank group.The above daily gastric infusion of each group is primary, continuous gavage administration observation 14d.
Testing index: each group mouse is observed daily, if having phenomena of mortality appearance, record The dead quantity and calculate
Its death rate.
Experimental calculation:
(1) median lethal dose is calculated using following formula (1):
LogLD50=Xm-i (p-0.5) formula (1)
Wherein, LD50 is used to characterize the logarithm of highest lethal dose for characterizing median lethal dose, Xm, and i is for characterizing phase
The logarithmic difference of adjacent dosage, ∑pFor characterizing the summation of each group male and female death rate.
(2) standard error of median lethal dose is calculated using following formula (2):
Wherein, SE50 is used to characterize the standard error of median lethal dose, and n is used to characterize every group of number of animals,
∑p2 for characterizing the sum of each group male and female death rate square value.
Experimental result:
Table 1: influence of the tobacco active component to mouse death rate
Note: dosage=tobacco active component quality/10g mouse weight
By table 1 and experimental calculation method, female mice LD50=34.05mg/10g, male mouse LD50=25.76mg/ are calculated to obtain
10g.Female mouse LD50 is greater than male mouse LD50, male and female LD50 average value=29.905mg/10g, according to Related product toxicology
Index (natural extract) LD50=2500~5000mg/kg belongs to hypotoxicity product.Illustrate that tobacco active component belongs to low toxicity
Component.
Embodiment 7: inhibit the test of human lung cancer A549 cell growth
Cell culture: it in 5%CO2,37 DEG C, under saturated humidity, is trained with (10%FBS+1%PS) culture medium of RPMI 1640
Human lung cancer A549 cell is supported, chooses the cell of logarithmic phase growth as experimental cell.It is diluted to after cell count with culture medium
Certain density cell suspension.
Cell growth status monitoring: cell real-time monitor is put into 37 DEG C, 5%CO2, in saturated humidity incubator.Take 8
Orifice plate, every hole are added 150 μ L RPMI, 1640 culture medium, are put into cell real-time monitor and walk baseline, take out after covering baseline
Dilute good 345 μ L of A549 cell suspension is added in octal plate, every hole, until every hole cell number about 2 × 104,30min is stood, is being fallen
Whether uniform set microscopically observation cell.The drug (the tobacco active component that embodiment 1 obtains) that 5 μ L have diluted is added in every hole
To final concentration of 50 μ g/ml, the culture medium containing 1%DMSO is put into cell real-time monitor and detects as blank control group, examines
It is photographed to record when survey finishes.
Blank control wells: being not added drug, adds 1%DMSO；
Experimental port: dosing object.
Experimental result is as shown in Figure 4: top line is blank control wells, lower section line is experimental port.
According to the proliferation growth curve of A549 cell, illustrate that tobacco active component of the present invention makees A549 cell growth inhibition
With.
Embodiment 8: inhibit the test of human liver cancer Hep-G2 cell growth
Cell culture: in 5%CO2,37 DEG C, under saturated humidity, with DMEM (10%FBS, 1%PS) culture medium culture people's lung
Cancer Hep-G2 cell chooses the good cell of growth conditions as experimental cell.It is diluted to centainly after cell count with culture medium
The cell suspension of concentration.
Cell growth status monitoring: cell real-time monitor is put into 5%CO2, in 37 DEG C of saturated humidity incubators.Take eight
Orifice plate, every hole are added 150 μ LDMEM culture mediums, are put into cell real-time monitor and walk baseline, take out octal plate after covering baseline,
The 345 μ L of Hep-G2 cell suspension diluted is added in every hole, until every hole cell number about 4 × 104,30min is stood, it is aobvious being inverted
Whether micro- microscopic observation cell is uniform.Drug (the tobacco active component that embodiment 1 obtains) that 5 μ L have diluted is added to end in every hole
Concentration is 50 μ g/mL, and the culture medium containing 1%DMSO is put into cell real-time monitor and detects, detected as blank control group
It is photographed to record when complete.
Blank control wells: being not added drug, and 1%DMSO is added；
Experimental port: dosing object.
Experimental result is as shown in Figure 5: top line is blank control wells, lower section line is experimental port.
According to the proliferation growth curve of Hep-G2 cell, illustrates that tobacco active component of the present invention grows Hep-G2 cell and press down
Production is used.
The above is merely a preferred embodiment of the present invention, it is noted that for those skilled in the art
For, under the premise of not departing from core of the invention technology, improvements and modifications can also be made, these improvements and modifications are also answered
Belong to scope of patent protection of the invention, and any change in the comparable meaning and scope of claims of the present invention, all
It is considered as including in Claims scope.
Claims (8)
1. a kind of extracting method of tobacco active component, which comprises the following steps:
(1) it pre-processes: drying and processing being carried out to tobacco material, and the tobacco material after drying and processing is carried out at ultramicro grinding
Reason, obtains tobacco Ultramicro-powder；
(2) normal-temperature water mentions: Extraction solvent being added in tobacco Ultramicro-powder, extracts operation under normal temperature conditions；It is grasped to extracting
Tobacco Ultramicro-powder after work carries out centrifugal treating, and collects the sediment after centrifugal treating；Extraction solvent in step (2) is pure
Change water；
(3) high-temperature water mentions: Extraction solvent is added in the sediment collected in step (2), under 90-100 DEG C of Extracting temperature
Carry out concentration extraction operation；Cooling processing, centrifugal treating are carried out to the concentrated extracting solution obtained after concentration extraction operation, and collected
Sediment after centrifugal treating；Extraction solvent in step (3) is purified water；
(4) methanol extracts: Extraction solvent is added in the sediment obtained in step (3), carries out under 0-4 DEG C of Extracting temperature
Extraction operation is extracted, centrifugal treating is carried out to Soakage extraction object, and collect the supernatant after centrifugal treating, the supernatant of collection is carried out
Drying and processing is concentrated in rotary evaporation, obtains tobacco active component；The methanol that Extraction solvent in step (4) is 85-100%.
2. extracting method according to claim 1, which is characterized in that
Drying and processing in step (1) is to dry tobacco material placement in an oven, so that the tobacco after drying and processing
The water content of raw material is less than 5%, and drying temperature is 50-60 DEG C, baking time 4-8h；
And/or the ultramicro crushing treatment in step (1) is that the tobacco material after drying and processing is used the powder of 50-80 mesh screen
Broken machine carries out pulverization process, and to the tobacco material after pulverization process, is carried out at crushing using the pulverizer of 200-400 mesh screen
Reason, obtains the tobacco Ultramicro-powder.
3. extracting method according to claim 1, which is characterized in that
The quality of addition Extraction solvent is 2-4 times of the quality of the tobacco Ultramicro-powder in step (2)；
And/or the extraction operation in step (2) refers to the tobacco Ultramicro-powder and Extraction solvent is placed in extractor, extracts
Time is 0.5-3h；
And/or the centrifugal treating in step (2) refers to: centrifuging temperature is 8-12 DEG C, centrifugation time 0.5-1.5h, and revolving speed is
3000-6000rpm/min。
4. extracting method according to claim 1, which is characterized in that
The quality of addition Extraction solvent is 1.2-2.4 times of the quality of sediment in step (3)；
And/or the extraction time of the concentration extraction operation in step (3) is 0.5-1.5h；
And/or the centrifugal treating in step (3) refer to will cooling treated that the concentrated extracting solution is placed in centrifugal bottle, from
Heart temperature is 8-12 DEG C, centrifugation time 0.5-1.5h, revolving speed 3000-6000rpm/min.
5. extracting method according to claim 1, which is characterized in that
The quality of addition Extraction solvent is 1.5-2.5 times of the quality of sediment in step (4)；
And/or the Soakage extraction operation in step (4) refers to, extraction time 12-24h primary every 0.5h stirring；
And/or the centrifugal treating in step (4) refers to the Soakage extraction object is placed in centrifugal bottle, centrifuging temperature are as follows: 3-5
DEG C, centrifugation time 0.5-1.5h, revolving speed 3000-6000rpm/min；
And/or the temperature of the concentration drying and processing in step (4) is 60-70 DEG C.
6. the tobacco active component that any extracting method is extracted in -5 according to claim 1.
7. application of the tobacco active component in preparation antibacterials according to claim 6.
8. tobacco active component is preparing the application in anticancer drug according to claim 6.
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