CN104873545A - Preparation method of galla chinensis activity extract and application of galla chinensis activity extract in inhibition of helicobacter pylori - Google Patents
Preparation method of galla chinensis activity extract and application of galla chinensis activity extract in inhibition of helicobacter pylori Download PDFInfo
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- CN104873545A CN104873545A CN201510276758.2A CN201510276758A CN104873545A CN 104873545 A CN104873545 A CN 104873545A CN 201510276758 A CN201510276758 A CN 201510276758A CN 104873545 A CN104873545 A CN 104873545A
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Abstract
The invention relates to a preparation method of a galla chinensis activity extract and an application of galla chinensis activity extract in inhibition of helicobacter pylori. The preparation method comprises the following steps: (1), taking baked or air dried galla chinensis, crushing by using a crushing machine; (2), weighing and taking galla chinensis powder, adding ethyl alcohol which is 50-95% in mass fraction for reflux extraction at 50-70 DEG C, wherein the massic volume ratio (kg/L) of galla chinensis to ethyl alcohol is 1:5-1:25, performing water bath extraction for 1-3 h for 1-2 times, so as to obtain galla chinensis extracting solution; (3), combining the galla chinensis extracting solution, filtering while the galla chinensis extracting solution is hot, centrifuging, collecting supernatant liquid and performing suction filtration; (4), concentrating the filter liquor at 45 DEG C, diluting with ethanol solution which is 25% in mass fraction, performing pasteurization, cooling and preserving in a sealing manner at 4 DEG C. According to the preparation method of the galla chinensis activity extract, the galla chinensis activity extract has an effect of inhibiting helicobacter pylori, is a natural and plant derived activity extract, can serve as a raw material or a product to be applied to health food and drugs; the preparation method is simple and low in cost.
Description
Technical field
The invention belongs to technical field of medicine preparation.
Background technology
Modern society's rhythm of life is accelerated, operating pressure is large and dietary habit is unhealthy, exacerbates the incidence rate of related gastrointestinal tract diseases, and wherein the M & M of gastric cancer remains high for a long time, greatly threatens the health of people.Helicobacter pylori (Helicobacter pylori) is called for short Hp, is the antibacterial of a kind of one pole, many flagellums, the blunt circle of end, helically bent, normal in typical helical form or arc on gastric epithelial cell surface.Helicobacter pylori infections is the main pathogenic of chronic gastritis, ulcer and gastric mucosa-associated lymphoid tissue tumor, and particularly increasing data shows that helicobacter pylori infections and incidence gastric cancer have very strong dependency.Therefore, find or research and develop effective pyloric spirobacterium inhibitor and there is good application prospect, particularly from the resource of natural product source, screen functional component.
Illustrate according to " Chinese Pharmacopoeia ", Galla Chinensis is Aceraceae plant Fructus rhois chinensis
rhus chinensismill., potanin sumac
rhus potaniniior Radix Rhois Sinicae Maxim.
rhus punjabensisstew. var.
sinica(Diels) insect gall on Rehd.et Wils. leaf, primarily of melaphis chinensis Baker
melaphis chinensis(Bell) Baker is parasitic and formed.Autumn plucks, and puts in boiling water bath and slightly boils or steam to surperficial gray, kill aphid, takes out, dry.Traditional Chinese Medicine thinks that Galla Chinensis has the effects such as pathogenic fire reducing of astringing the lung, relieving diarrhea with astringents, arresting sweating hemostasis, and modern pharmacological research thinks that it also has the effect such as antiinflammatory, sterilization.Chinese patent CN101433557B discloses a kind of Galla Chinensis active component extracting method and the toxic action to tumor cell line thereof, Chinese patent CN101107934B discloses a kind of antibacterial and the combination thereof that contain Galla Chinensis extract, and this antibacterial has good prevention effect to multiple kinds of crops disease and pest.But about the preparation of Galla Chinensis ethanol extract is in particular to the inhibitory action to helicobacter pylori, there is not been reported.
Summary of the invention
The object of the invention is provide a kind of preparation method of Galla Chinensis activity extract and suppressing the application in helicobacter pylori.
The present invention is achieved through the following technical solutions.
The preparation method of a kind of Galla Chinensis activity extract of the present invention, is characterized in that as follows.
(1) Galla Chinensis (fruit) got a certain amount of oven dry or naturally dry, stand-by after Chinese medicine grinder is pulverized.
(2) take step (1) preparation-obtained Chinese Gall, add the alcohol reflux of 50-95% mass fraction, the solid-liquid ratio of Galla Chinensis and ethanol is 1:5 ~ 1:25(kg/L, mass volume ratio), in 50-70
oat C temperature, 1 ~ 3 h is extracted in water-bath, and extraction time is 1 ~ 2 time, obtains Chinese nutgall.
(3) extracting solution in step (2) is merged, filtered while hot, then centrifugal 15 min under 4800 r/m rotating speeds, collect the supernatant and again carry out sucking filtration.
(4) filtrate in step (3) be placed on Rotary Evaporators concentrated, thickening temperature is 45
oc, is finally diluted to finite concentration with the alcoholic solution that mass fraction is 25%, cools after pasteurization, preserves, can obtain Galla Chinensis activity extract in 4 DEG C of condition lower seals.
Galla Chinensis activity extract of the present invention is suppressing the application in helicobacter pylori.
Advantage of the present invention is: provide the preparation method containing Galla Chinensis activity extract, and the extract that the present invention prepares as raw material or products application in aspects such as relevant medicines, can be conducive to Galla Chinensis exploitation and value promotion; The Galla Chinensis extract prepared has the effect suppressing growth of H. pylori preferably, is a kind of activity extract of natural, plant source; Preparation technology is simple, and cost is low, has wide DEVELOPMENT PROSPECT.
Detailed description of the invention
Be clearly and completely described technical scheme of the present invention below, obviously, described embodiment is only the present invention's part embodiment, instead of whole embodiments.When not departing from the above-mentioned thought of the present invention, method, the various replacement method made according to ordinary skill knowledge and conventional means or change, be all included within the present invention.
Embodiment 1.
The Galla Chinensis got a certain amount of oven dry or naturally dry, stand-by after Chinese medicine grinder is pulverized.Take Chinese Gall 70 g of above-mentioned preparation, add the alcohol reflux of 95% mass fraction, solid-liquid ratio is 1:10(kg/L, mass volume ratio), in 70
owater-bath reflux, extract, 2 h at C temperature, extraction time is 2 times, merge extractive liquid, filtered while hot, then centrifugal 15 min under 4800 r/m rotating speeds, collects the supernatant and again carries out sucking filtration.Above-mentioned filtrate be placed on Rotary Evaporators concentrated, thickening temperature is 45
oc, is finally 0.35 kg/L by the crude drug mass concentration that the alcoholic solution that mass fraction is 25% is diluted to gained sample extracting solution corresponding, cools after pasteurization, preserves, can obtain Galla Chinensis activity extract A in 4 DEG C of condition lower seals.
Embodiment 2.
The Galla Chinensis got a certain amount of oven dry or naturally dry, stand-by after Chinese medicine grinder is pulverized.Take Chinese Gall 20 g of above-mentioned preparation, add the alcohol reflux of 60% mass fraction, solid-liquid ratio is 1:20(kg/L, mass volume ratio), in 50
owater-bath reflux, extract, 1 h at C temperature, extraction time is 2 times, merge extractive liquid, filtered while hot, then centrifugal 15 min under 4800 r/m rotating speeds, collects the supernatant and again carries out sucking filtration.Above-mentioned filtrate be placed on Rotary Evaporators concentrated, thickening temperature is 45
oc, is finally 1 kg/L by the crude drug mass concentration that the alcoholic solution that mass fraction is 25% is diluted to gained sample extracting solution corresponding, cools after pasteurization, preserves, can obtain Galla Chinensis activity extract B in 4 DEG C of condition lower seals.
Embodiment 3.
The Galla Chinensis got a certain amount of oven dry or naturally dry, stand-by after Chinese medicine grinder is pulverized.Take Chinese Gall 20 g of above-mentioned preparation, add the alcohol reflux of 75% mass fraction, solid-liquid ratio is 1:15(kg/L, mass volume ratio), in 60
owater-bath reflux, extract, 3 h at C temperature, extraction time is 1 time, merge extractive liquid, filtered while hot, then centrifugal 15 min under 4800 r/m rotating speeds, collects the supernatant and again carries out sucking filtration.Above-mentioned filtrate be placed on Rotary Evaporators concentrated, thickening temperature is 45
oc, is finally 1 kg/L by the crude drug mass concentration that the alcoholic solution that mass fraction is 25% is diluted to gained sample extracting solution corresponding, cools after pasteurization, preserves, can obtain Galla Chinensis activity extract C in 4 DEG C of condition lower seals.
Below in conjunction with specific experiment, the Galla Chinensis activity extract vitro inhibition helicobacter pylori activity that this method prepares is described.
1, helicobacter pylori source.
Select from outpatient service patients with gastric disease gastric mucosa specimen, the international standard strains A TCC11637 that laboratory is preserved.
Through electronic gastroscopy, get gastric antrum lesser curvature side apart from pyloric ostium about 5 cm place gastric mucosa tissue 1 piece, carry out gastric mucosa tissue helicobacter pylori (Hp) and cultivate, cultivate in positive patient at Hp, add and do drug sensitivity test.
2, Hp cultivates and qualification.
Tissue grinder gastric tissue being placed in sterilizing grinds to form mixing liquid, uses sterilizing dropper, aspirates tissue mixing liquid, is inoculated in containing on 10% Sheep Whole Blood Columbia agar flat board, 37 DEG C, at 5%O
2, 10%CO
2and 85%N
2environment in cultivate 4 days, observed result.Result criterion: Gram's staining is negative, oxidase test is positive, and catalase test is positive, and urease test is positive, and triphenyltetrazolium chloride test bacterium colony on slant medium presents metallic luster, and meeting above whole person is that Hp is positive.
3, drug effect experiment.
Choose the clinical strains of separation, bacterial strain is carried out going down to posterity 3 ~ 4 times, needed for amplification culture to experiment, be inoculated in containing on 10% Sheep Whole Blood Columbia agar flat board, punching, adds Galla Chinensis ethanol extraction continuation cultivation and observes drug sensitive experiment result afterwards in 3 days, measure antibacterial circle diameter.
4, experimental result.
Table 1 Galla Chinensis activity extract is to helicobacter pylori In-vitro Inhibitory Effect (n=3)
| A | B | C | 25% ethanol control group | |
| Inhibition zone size (mm) | 43.7±1.2 | 45.5±14.8 | 43.0±12.7 | 13.5±2.1 |
Experiment conclusion:
Adopt different ethanol concentration to extract Galla Chinensis extract A, B and C of obtaining, compared with 25% ethanol control group, all there is in experiment in vitro the effect suppressing growth of H. pylori preferably.
Claims (2)
1. a preparation method for Galla Chinensis activity extract, is characterized in that as follows:
(1) a certain amount of oven dry is got or the Galla Chinensis that naturally dries, stand-by after Chinese medicine grinder is pulverized;
(2) step (1) preparation-obtained Chinese Gall is taken, add the alcohol reflux of 50-95% mass fraction, the solid-liquid ratio (kg/L) of Galla Chinensis and ethanol is 1:5 ~ 1:25, and at 50-70 DEG C of temperature, 1 ~ 3 h is extracted in water-bath, extraction time is 1 ~ 2 time, obtains Chinese nutgall;
(3) extracting solution in step (2) is merged, filtered while hot, then centrifugal 15 min under 4800 r/m rotating speeds, collect the supernatant and again carry out sucking filtration;
(4) filtrate in step (3) be placed on Rotary Evaporators concentrated, thickening temperature is 45 DEG C, is finally the alcoholic solution dilution of 25% with mass fraction, cools, preserve, can obtain Galla Chinensis activity extract in 4 DEG C of condition lower seals after pasteurization.
2. Galla Chinensis activity extract according to claim 1 is suppressing the application in helicobacter pylori.
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Cited By (2)
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|---|---|---|---|---|
| CN105079289A (en) * | 2015-09-07 | 2015-11-25 | 北京大学第一医院 | Application of Chinese gall leaven in preparation of anti-helicobacter-pylori drug |
| CN112369585A (en) * | 2020-11-03 | 2021-02-19 | 湖南省明园蜂业有限公司 | Application of gallnut honey in preparation of anti-helicobacter pylori product |
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| CN105079289A (en) * | 2015-09-07 | 2015-11-25 | 北京大学第一医院 | Application of Chinese gall leaven in preparation of anti-helicobacter-pylori drug |
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| CN112369585A (en) * | 2020-11-03 | 2021-02-19 | 湖南省明园蜂业有限公司 | Application of gallnut honey in preparation of anti-helicobacter pylori product |
| CN112369585B (en) * | 2020-11-03 | 2022-05-24 | 湖南省明园蜂业有限公司 | Application of gallnut honey in preparation of anti-helicobacter pylori product |
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