CN110946926A - Development and application of natural bactericidal ointment - Google Patents
Development and application of natural bactericidal ointment Download PDFInfo
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- CN110946926A CN110946926A CN201911346254.8A CN201911346254A CN110946926A CN 110946926 A CN110946926 A CN 110946926A CN 201911346254 A CN201911346254 A CN 201911346254A CN 110946926 A CN110946926 A CN 110946926A
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- 239000002674 ointment Substances 0.000 title claims abstract description 45
- 230000000844 anti-bacterial effect Effects 0.000 title claims description 17
- 238000011161 development Methods 0.000 title abstract description 5
- 235000002637 Nicotiana tabacum Nutrition 0.000 claims abstract description 41
- 241000208125 Nicotiana Species 0.000 claims abstract description 40
- 239000000284 extract Substances 0.000 claims abstract description 23
- 239000002994 raw material Substances 0.000 claims abstract description 18
- 239000002904 solvent Substances 0.000 claims abstract description 12
- 238000000034 method Methods 0.000 claims abstract description 7
- 238000000605 extraction Methods 0.000 claims description 21
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims description 15
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 14
- WPYMKLBDIGXBTP-UHFFFAOYSA-N benzoic acid Chemical compound OC(=O)C1=CC=CC=C1 WPYMKLBDIGXBTP-UHFFFAOYSA-N 0.000 claims description 14
- 238000011049 filling Methods 0.000 claims description 13
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 10
- 238000005119 centrifugation Methods 0.000 claims description 9
- DTGKSKDOIYIVQL-WEDXCCLWSA-N (+)-borneol Chemical compound C1C[C@@]2(C)[C@@H](O)C[C@@H]1C2(C)C DTGKSKDOIYIVQL-WEDXCCLWSA-N 0.000 claims description 7
- REPVLJRCJUVQFA-UHFFFAOYSA-N (-)-isopinocampheol Natural products C1C(O)C(C)C2C(C)(C)C1C2 REPVLJRCJUVQFA-UHFFFAOYSA-N 0.000 claims description 7
- 239000005711 Benzoic acid Substances 0.000 claims description 7
- 235000010233 benzoic acid Nutrition 0.000 claims description 7
- 229940116229 borneol Drugs 0.000 claims description 7
- CKDOCTFBFTVPSN-UHFFFAOYSA-N borneol Natural products C1CC2(C)C(C)CC1C2(C)C CKDOCTFBFTVPSN-UHFFFAOYSA-N 0.000 claims description 7
- DTGKSKDOIYIVQL-UHFFFAOYSA-N dl-isoborneol Natural products C1CC2(C)C(O)CC1C2(C)C DTGKSKDOIYIVQL-UHFFFAOYSA-N 0.000 claims description 7
- BEFDCLMNVWHSGT-UHFFFAOYSA-N ethenylcyclopentane Chemical compound C=CC1CCCC1 BEFDCLMNVWHSGT-UHFFFAOYSA-N 0.000 claims description 7
- 238000002360 preparation method Methods 0.000 claims description 7
- 235000010199 sorbic acid Nutrition 0.000 claims description 7
- 239000004334 sorbic acid Substances 0.000 claims description 7
- 229940075582 sorbic acid Drugs 0.000 claims description 7
- 235000019441 ethanol Nutrition 0.000 claims description 6
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 claims description 5
- 229920002565 Polyethylene Glycol 400 Polymers 0.000 claims description 5
- 229920001030 Polyethylene Glycol 4000 Polymers 0.000 claims description 5
- 238000003756 stirring Methods 0.000 claims description 4
- 238000002390 rotary evaporation Methods 0.000 claims description 3
- 241000196324 Embryophyta Species 0.000 claims description 2
- 230000009467 reduction Effects 0.000 claims description 2
- 239000002798 polar solvent Substances 0.000 claims 2
- 238000007598 dipping method Methods 0.000 claims 1
- 239000000419 plant extract Substances 0.000 claims 1
- 238000004659 sterilization and disinfection Methods 0.000 abstract description 18
- 238000004519 manufacturing process Methods 0.000 abstract description 6
- 238000011031 large-scale manufacturing process Methods 0.000 abstract description 4
- 208000017520 skin disease Diseases 0.000 abstract description 4
- 230000036559 skin health Effects 0.000 abstract description 4
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- 238000012545 processing Methods 0.000 abstract description 2
- 235000019505 tobacco product Nutrition 0.000 abstract description 2
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 15
- 239000000523 sample Substances 0.000 description 12
- 238000002156 mixing Methods 0.000 description 8
- 230000003385 bacteriostatic effect Effects 0.000 description 6
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- 238000002474 experimental method Methods 0.000 description 5
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- 239000000706 filtrate Substances 0.000 description 2
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- 239000000401 methanolic extract Substances 0.000 description 2
- 238000012946 outsourcing Methods 0.000 description 2
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- 239000013074 reference sample Substances 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- 238000003809 water extraction Methods 0.000 description 2
- 238000005303 weighing Methods 0.000 description 2
- 206010017553 Furuncle Diseases 0.000 description 1
- 244000061176 Nicotiana tabacum Species 0.000 description 1
- 238000012356 Product development Methods 0.000 description 1
- 208000002474 Tinea Diseases 0.000 description 1
- 241000130764 Tinea Species 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000002070 germicidal effect Effects 0.000 description 1
- 238000007654 immersion Methods 0.000 description 1
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- 230000002458 infectious effect Effects 0.000 description 1
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- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- VYQNWZOUAUKGHI-UHFFFAOYSA-N monobenzone Chemical compound C1=CC(O)=CC=C1OCC1=CC=CC=C1 VYQNWZOUAUKGHI-UHFFFAOYSA-N 0.000 description 1
- 244000045947 parasite Species 0.000 description 1
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/81—Solanaceae (Potato family), e.g. tobacco, nightshade, tomato, belladonna, capsicum or jimsonweed
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
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- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/0012—Galenical forms characterised by the site of application
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
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Abstract
The invention establishes development and application of a natural sterilization ointment, and belongs to the technical field of tobacco product processing. The natural tobacco branch fine extract is obtained by crushing tobacco branches at ultralow temperature, extracting with a solvent and centrifuging at high speed, and the fine extract is prepared into the sanitary disinfection ointment by adopting a certain process. The natural raw materials of the disinfection sanitary products are obtained by utilizing the tobacco branches for the first time, the production process of the natural disinfection ointment is creatively established, the large-scale production is realized, the natural disinfection ointment can be applied to the fields of skin health care, skin disinfection, skin disease treatment and the like, and the high-added-value utilization of the tobacco branches with natural disinfection is realized.
Description
Technical Field
The invention relates to the technical field of tobacco branch product development, in particular to development and application of a natural sterilization ointment.
Background
Tobacco, whole plant of Nicotiana tabacum L. The ancient books record that the traditional Chinese medicine composition has the effects of relieving swelling, removing toxicity and killing parasites, and can be used for treating furuncle pyogenic infections, tinea capitis, tinea alba and tinea capitis. A natural sanitary disinfection ointment is developed independently by taking tobacco branches as raw materials and extracting antibacterial components, and can be used for skin health care, skin disinfection and skin disease treatment.
Disclosure of Invention
The invention establishes a production process of natural bactericidal ointment and realizes large-scale production, and the product can be used for skin health care, skin disinfection and skin disease treatment and comprises the following steps:
1. preparation of tobacco branch fine extract
(a) Pretreatment: drying the tobacco branch raw material, coarsely crushing the dried tobacco branch raw material, and then performing superfine crushing treatment to obtain tobacco branch superfine powder;
(b) water extraction: adding an extraction solvent into the tobacco branch superfine powder, carrying out extraction operation at a certain temperature, carrying out centrifugal treatment on the tobacco branch superfine powder after the extraction operation, and collecting the lower-layer precipitate after the centrifugal treatment for later use; the extraction solvent in the step is purified water;
(c) methanol extraction: adding an extraction solvent into the precipitate obtained in the step (b), performing extraction operation at a certain temperature, performing centrifugal treatment on the extract after the extraction operation, collecting the supernatant after the centrifugal treatment, performing concentration treatment in a rotary steaming manner, and baking to obtain an extract to obtain a fine tobacco branch extract; the extraction solvent in step (a) is 85% methanol;
2. preparation of ointment
(a) Raw materials and raw material proportion
Tobacco branch extract, PEG-4000, PEG-400, glycerol, borneol, 95% ethanol, sorbic acid and benzoic acid;
sequentially adding PEG-4000, PEG-400, glycerol and the tobacco branch extract into a material mixing tank, and stirring for dissolving;
adding weighed ethanol into a constant-temperature water bath kettle, adding borneol, benzoic acid and sorbic acid, heating in a water bath at a certain temperature to completely dissolve the borneol, the benzoic acid and the sorbic acid, and slowly pouring the mixture into a mixing tank to be uniformly stirred and mixed;
filtering at 80 deg.C, transferring filtrate into clean blending tank
(b) Filling: adding the material liquid in the material mixing tank into an ointment filling machine, adjusting filling pressure and filling amount, and filling;
(c) and (7) outsourcing and warehousing.
Preferably, the ultrafine grinding treatment in the step (1) (a) is to perform coarse grinding on the dried tobacco branch raw material, then perform ultrafine grinding treatment, and perform grinding treatment (500 meshes) by using an ultrafine grinder to obtain the tobacco branch ultrafine powder.
Preferably, the centrifugation treatment in the steps (1) (b) is to place the concentrated extract solution after temperature reduction treatment in a centrifuge bottle, wherein the centrifugation time is 30min and the rotation speed is 3500 rpm/min.
Preferably, the volume ratio of the added extraction solvent in the step (1) (b) is 1:2, the extraction temperature is 90 ℃, the extraction time is 30min, and the centrifugation temperature is 8 ℃.
Preferably, the immersion extraction temperature in the step (1) (c) is 4 ℃, the extraction time is 8h, the centrifugation time is 4 ℃, and the rotary evaporation time is 60 ℃.
Preferably, the stirring dissolution temperature in the step (2) (b) is 80 ℃, and the dissolution time is 10 min.
Preferably, the solvent in step (2) (c) is 95% ethanol, and the temperature of the water bath is 70 ℃.
The invention establishes the development and application of a natural sterilization ointment, and belongs to the technical field of tobacco product processing. The natural tobacco branch fine extract is obtained by crushing tobacco branches at ultralow temperature, extracting with a solvent and centrifuging at high speed, and the fine extract is prepared into the sanitary disinfection ointment by adopting a certain process. The natural raw materials of the disinfection sanitary products are obtained by utilizing the tobacco branches for the first time, the production process of the natural disinfection ointment is creatively established, the large-scale production is realized, the natural disinfection ointment can be applied to the fields of skin health care, skin disinfection, skin disease treatment and the like, and the high-added-value utilization of the tobacco branches with natural disinfection is realized.
The following description is only an overview of the technical solutions of the present invention, and in order to make the technical means of the present invention more clearly understood and to be implemented according to the content of the description, the preferred embodiments of the present invention are described below.
Drawings
FIG. 1 is a flow chart of the manufacturing process of the natural ointment of the present invention;
FIG. 2 is a product diagram of the natural germicidal ointment of the present invention;
FIG. 3 shows the experimental results of the natural bactericidal ointment of the present invention for inhibiting Escherichia coli;
FIG. 4 shows the results of experiments on the inhibition of Staphylococcus aureus by the natural bactericidal ointment of the present invention;
FIG. 5 shows the experimental results of the natural bactericidal ointment of the present invention inhibiting Bacillus subtilis.
Detailed Description
The present invention will be described in further detail with reference to examples. It is to be understood that the following detailed description is exemplary and is intended to provide further explanation of the invention as claimed. Unless defined otherwise, all scientific and technical terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs.
The invention provides a production process of natural bactericidal powder and realizes large-scale production, and the production process refers to fig. 1, and specifically comprises the following steps:
1. preparation of tobacco branch refined extract
(1) Pretreatment of
Weighing 30Kg of tobacco branch raw material, drying at 60 ℃ until the water content of the tobacco branches is less than 5%, roughly crushing the dried tobacco branch raw material, and then carrying out superfine crushing to obtain the tobacco branch superfine powder (500 meshes).
(2) Water extraction
Adding tobacco branch micropowder into 200L concentration extraction tank, adding 60Kg purified water, concentrating and extracting at 90 deg.C for 30min, centrifuging the extract at 8 deg.C at 3500rpm/min for 30min, and collecting the lower precipitate after centrifugation.
(3) Methanol extraction
Extracting the precipitate with water, adding 4Kg of 85% methanol, soaking and extracting at 4 deg.C for 8 hr, centrifuging at 4 deg.C and 3500rpm/min for 30min, collecting the supernatant, concentrating at 60 deg.C by rotary evaporation, and drying to obtain tobacco branch extract.
2. Ointment preparation
(1) Preparation of raw materials
1.1 raw materials and proportion: tobacco branch extract, PEG-4000, PEG-400, glycerol, borneol, 95% ethanol, sorbic acid and benzoic acid;
1.2 sequentially adding PEG-4000, PEG-400, glycerol and the tobacco branch extract into a proportioning tank, and stirring and dissolving for 10min at 80 ℃;
1.3 adding the weighed 95% ethanol into a constant-temperature water bath kettle, then adding borneol, benzoic acid and sorbic acid, heating in a water bath at 70 ℃ to completely dissolve the borneol, the benzoic acid and the sorbic acid, and then slowly pouring the mixture into a mixing tank to be uniformly stirred and mixed;
filtering at 1.480 deg.C, and transferring the filtrate into clean blending tank.
(2) Filling
Adding the material liquid in the material mixing tank into an ointment filling machine, adjusting filling pressure and filling amount, and filling.
(3) Outsourcing and warehousing
Example 1 was carried out: detection of ointment bacteriostatic activity
Whether the tobacco branch ointment (hereinafter referred to as the ointment) can inhibit the growth of escherichia coli, staphylococcus aureus and bacillus subtilis is examined.
1. Principle of experiment
The effect of the ointment on the growth of bacteria was examined by using the continuous dissolution of the ointment and the formation of different concentration gradients through diffusion of agar. Whether the bacteriostatic ring has bacteriostatic ability is judged by measuring the diameter of the bacteriostatic ring, when the diameter is more than or equal to 7mm, the sample has bacteriostatic action, and if the diameter is less than 7mm, the sample has no bacteriostatic action.
2. Experimental methods
2.1 Experimental groups
Sample group: weighing 5g of tobacco ointment, adding 3ml of methanol, and mixing to obtain methanol extract of the ointment.
Blank control group: methanol
2.2 preparation of Experimental Filter paper sheet
5 pieces of sterile dried filter paper were taken, wherein 4 pieces of the sample group and 1 piece of the blank control group were used. Dripping 20 μ l of methanol extract of ointment into the sample group, dripping 20 μ l of methanol into the blank control group, then placing the filter paper sheet in a clean sterile plate, and opening the cover to place in an ultra-clean workbench for natural drying for later use.
2.3 inoculation
Taking the concentration of 5X 10 by using a pipettor5cfu/ml～5×106cfu/ml Escherichia coli, Staphylococcus aureus, Bacillus subtilis suspension 200 ul in tryptone soy agar medium plate surface, immediately using the coating rod evenly spread. The plate was covered and dried at room temperature for 5 min.
2.4 paste-on ointment
Each test is stuck with 1 infectious bacterium plate, and each plate is stuck with 4 test sample plates and 1 blank reference sample plate, and the number of the test sample plates and the blank reference sample plates is 5. A sample was taken with sterile forceps and placed on the surface of the plate. The distance between the centers of the various pieces is more than 25mm, and the distance between the centers of the various pieces and the periphery of the flat plate is more than 15 mm. The sample wafer is lightly pressed with sterile forceps to be tightly attached to the surface of the flat plate. The plate was covered, placed in a 37 ℃ incubator, and the results were observed after 16 hours of incubation. The diameter of the zone was measured with a ruler and recorded (the zone was chosen for uniform and complete aseptic growth, bounded by the outer edge of the zone, and the diameter was measured, including the patch).
3. Results of the experiment
The tobacco ointment has the effects of inhibiting growth of Escherichia coli, Staphylococcus aureus, and Bacillus subtilis.
The experimental results are statistically as follows:
(1) the natural bactericidal ointment results in colibacillus resisting experiment, where a, b, c and d are ointment sample groups and e is blank control group
(2) The natural bactericidal ointment has the test result of resisting staphylococcus aureus, wherein a, b, c and d are ointment sample groups, and e is a blank control group
(3) The experimental result of the natural bactericidal ointment for resisting bacillus subtilis shows that a, b, c and d are ointment sample groups, and e is a blank control group
While the foregoing is directed to the preferred embodiment of the present invention, it will be understood by those skilled in the art that various changes and modifications may be made therein without departing from the spirit and scope of the invention as defined by the appended claims.
Claims (6)
1. The preparation method of the natural bactericidal ointment is characterized by comprising the following steps:
(1) the step (1) is plant refined extract;
(a) the extraction solvent in the step (1) (b) is a strong polar solvent;
(b) the extraction solvent in the step (1) (c) is a medium polar solvent;
(2) in the step (2) (a) of the specification, the ointment raw materials are added or reduced or are mixed according to any proportion by using tobacco branch extract or plant extract, PEG-4000, PEG-400, glycerol, borneol, 95% ethanol, sorbic acid, benzoic acid and the like;
(3) the filling in the step (2) (b) is conventional filling or intelligent filling.
2. The method for preparing the natural bactericidal ointment by taking the fine extract of the tobacco branches as the raw material according to claim 1, which is characterized by comprising the following steps: the centrifugation treatment in the step (1) (a) is to place the concentrated extracting solution after temperature reduction treatment in a centrifuge bottle, wherein the centrifugation time is 30min, and the rotation speed is 3500 rpm/min.
3. The method for preparing the natural bactericidal ointment by taking the fine extract of the tobacco branches as the raw material according to claim 1, which is characterized by comprising the following steps: in the step (1) (a), the volume ratio of the added extraction solvent is V/V-1: 2, the extraction temperature is 90 ℃, the extraction time is 30min, and the centrifugation temperature is 8 ℃.
4. The method for preparing the natural bactericidal ointment by taking the fine extract of the tobacco branches as the raw material according to claim 1, which is characterized by comprising the following steps: the dipping extraction temperature in the step (1) and the step (b) is 4 ℃, the extraction time is 8h, the centrifugation time is 4 ℃, and the rotary evaporation time is 60 ℃.
5. The method for preparing the natural bactericidal ointment by taking the fine extract of the tobacco branches as the raw material according to claim 1, which is characterized by comprising the following steps: the stirring and dissolving temperature in the step (2) is 80 ℃, and the dissolving time is 10 min.
6. The method for preparing the natural bactericidal ointment by taking the fine extract of the tobacco branches as the raw material according to claim 1, which is characterized by comprising the following steps: the solvent in the step (2) is 95% ethanol, and the water bath temperature is 70 ℃.
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109223897A (en) * | 2018-11-12 | 2019-01-18 | 贵州贵安精准医学研究院股份有限公司 | A kind of tobacco active component, extracting method and its application |
| CN109966370A (en) * | 2019-03-06 | 2019-07-05 | 贵州省烟草科学研究院 | A kind of tobacco active component, extracting method and its application |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109223897A (en) * | 2018-11-12 | 2019-01-18 | 贵州贵安精准医学研究院股份有限公司 | A kind of tobacco active component, extracting method and its application |
| CN109966370A (en) * | 2019-03-06 | 2019-07-05 | 贵州省烟草科学研究院 | A kind of tobacco active component, extracting method and its application |
Non-Patent Citations (1)
| Title |
|---|
| 贵州贵安精准医学研究院股份有限公司: "烟草及系列产品", 《HTTP://WWW.APMGA.CN/LISTS/SHOW/5/6/36.HTML》 * |
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