CN109207630A - It is a kind of for detecting the molecular labeling and application method of anti gibberellic disease QTL Qfhb.hbaas-1AS - Google Patents
It is a kind of for detecting the molecular labeling and application method of anti gibberellic disease QTL Qfhb.hbaas-1AS Download PDFInfo
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Abstract
The invention discloses a kind of for detecting the molecular labeling and application method of anti gibberellic disease QTL Qfhb.hbaas-1AS.The present invention provides the substances of the genotype in the site SNP IWB75039 of detection chromosome of wheat 1AS to identify or assist the application in identification wheat scab resistance；Additionally provide application of the substance of the genotype in the site SNP IWB75039 of detection chromosome of wheat 1AS in preparation identification or auxiliary identification wheat scab resistance product.The present invention has found the anti gibberellic disease site Qfhb.hbaas-1AS on wheat 1A the short arm of a chromosome by whole-genome association (GWAS), it is further associated with SNP IWB75039 and is converted into regular-PCR label M-1AS-Fhb, the label can be used for detecting the genotype of anti gibberellic disease QTL Qfhb.hbaas-1AS, and be used for anti gibberellic disease molecular breeding.
Description
Technical field
The present invention relates to field of biotechnology, and in particular to one kind is for detecting anti gibberellic disease QTL Qfhb.hbaas-1AS
Molecular labeling and application method.
Background technique
The wheat scab as caused by Fusarium graminearum etc. (Fusarium head blight, FHB) is a kind of stream extensively
Capable fungal disease, seriously affects yield and quality of wheat.Importantly, susceptible seed contains mycotoxin, as corruption is avenged in deoxidation
Sickle-like bacteria enol (Deoxynivalenol, DON), endangers human and livestock health, influences edible and feeding.
China middle and lower reach of Yangtze River and the northeast area of wheat be wheat scab often send out, retransmission region.In recent years, become by global climate
Change, the universal etc. of straw-returning influences under wheat-Corn Rotation System system, the disease is in expansion, exacerbation trend, it has also become Yellow River-Huai River region
Commonly-occurring disease evil.The current average annual occurring area of China's wheat scab is more than 533.3 ten thousand hm2, 2010-2015 causes average annual total damage
It loses and is up to 3,370,000 t, occupy first of wheat diseases.
Culture utilization disease-resistant variety is the economical and effective means for reducing head blight harm.Both at home and abroad to scab resistance heredity
It has conducted extensive research, has positioned about 100 anti gibberellic disease QTL, be distributed in all chromosomes of wheat, Fhb1-Fhb7 etc. 7 anti-
Ospc gene is by definite designation, wherein most strong and stable from the Fhb1 resistance that Sumai 3 is located at 3B the short arm of a chromosome.However, removing
Outside Fhb1, the report of other gene breeding applications is less.Therefore anti gibberellic disease site is further excavated, its molecular labeling pair is developed
It is significant in breeding for scab resistance.
Summary of the invention
It is an object of the invention to detect the substance of the genotype in the site SNP IWB75039 of chromosome of wheat 1AS
Purposes.
The present invention provides detection chromosome of wheat 1AS the site SNP IWB75039 genotype substance identification or
Application in auxiliary identification wheat scab resistance；
Or the present invention provides the substances of the genotype in the site SNP IWB75039 of detection chromosome of wheat 1AS to prepare
Application in identification or auxiliary identification wheat scab resistance product.
The present invention also provides detection chromosome of wheat 1AS the site SNP IWB75039 genotype substance resist it is red
Application in mildew wheat breeding；
Or, the present invention also provides the substances of the genotype in the site SNP IWB75039 of detection chromosome of wheat 1AS to exist
Prepare the application in wheat-resistance to scab breeding product.
In above-mentioned application, the site the SNP IWB75039 is the physical location 10.1Mb on chromosome of wheat 1AS
Site；
Or, the genotype in the site the SNP IWB75039 is GG or AA.
In above-mentioned application, the substance of the genotype in the site SNP IWB75039 of the detection chromosome of wheat 1AS is as follows
1) or 2):
1) primer set, primer set single strand dna as shown in sequence 1 in sequence table or derivatives thereof and sequence
Single strand dna shown in sequence 2 or derivatives thereof forms in list；
2) contain the PCR reagent or kit of the primer set.
In above-mentioned application, the derivative of single strand dna shown in sequence 1 is that sequence 1 is passed through one in the sequence table
The substitution of a or several nucleotide and with the DNA molecular with the same function of sequence 1；
The derivative of single strand dna shown in sequence 2 is that sequence 2 is passed through one or several nucleosides in the sequence table
Acid substitution and with the DNA molecular with the same function of sequence 2.
Another object of the present invention is to provide a kind of product.
Product provided by the invention is the genotype in the site SNP IWB75039 of above-mentioned detection chromosome of wheat 1AS
Substance；
The product has the function of following 1) or 2) at least one:
1) identify or assist identification wheat scab resistance；
2) wheat-resistance to scab breeding.
3rd purpose of the invention is to provide a kind of method identified or auxiliary identifies wheat scab resistance.
Method provided by the invention, the genotype for the site SNP IWB75039 of detection chromosome of wheat 1AS to be measured are
GG or AA judges the scab resistance of the wheat to be measured according to genotype；
The genotype in the site SNP IWB75039 is that the scab resistance of the wheat to be measured of GG is higher than SNP IWB75039
The genotype for the genotype selected is the wheat to be measured of AA.
4th purpose of the invention is to provide a kind of method of breeding wheat-resistance to scab.
Method provided by the invention, for detect chromosome of wheat 1AS the site SNP IWB75039 genotype be GG also
It is AA, breeding genotype is the wheat to be measured of GG, obtains the wheat of anti gibberellic disease.
In the above method, the genotype for detecting the site SNP IWB75039 of chromosome of wheat 1AS to be measured is GG or AA
Method be following A) or B):
A) 90K SNP chip is analyzed；
B PCR amplification) is carried out to the Wheat volatiles DNA to be measured with the primer set in claim 4, is detected
PCR product；
If PCR product is the segment of 389bp size, the genotype of the wheat SNP site IWB75039 to be measured is AA；
If the genotype of the wheat SNP site IWB75039 to be measured is GG without PCR product.
Above-mentioned PCR product is that the segment of 389bp size is detected by electrophoresis detection or direct Sequencing.
Any description above wheat can be but be not limited to 240 parts of wheat breeds shown in embodiment part table 1.
The present invention has found the anti gibberellic disease on wheat 1A the short arm of a chromosome by whole-genome association (GWAS)
Site Qfhb.hbaas-1AS explains phenotypic variation 5.6%, containing FHB index under 4 environment of disease-resistant allele material
BLUE value ratio contains susceptible allele material low 33.0%.The present invention is further associated with SNP IWB75039 and is converted into commonly
PCR marks M-1AS-Fhb, and the two is 96.7% to 240 parts of material parting concordance rates.The label can be used for detecting anti gibberellic disease
The genotype of QTL Qfhb.hbaas-1AS, and it is used for anti gibberellic disease molecular breeding.
Detailed description of the invention
Fig. 1 is that Qfhb.hbaas-1AS different genotype material FHB indexBLUE compares.
Fig. 2 is M-1AS-Fhb to part kind amplification.
Specific embodiment
Embodiment below facilitates a better understanding of the present invention, but does not limit the present invention.Experiment in following embodiments
Method is unless otherwise specified conventional method.Test material as used in the following examples is unless otherwise specified certainly
What routine biochemistry reagent shop was commercially available.Quantitative test in following embodiment is respectively provided with three repeated experiments, as a result makes even
Mean value.
Head blight pathogen Huang gang 1: bibliography: Zhu Zhanwang, Yang Lijun, Tong Hanwen wait Hubei Wheat kind
The scab resistance of (being) analyzes [J] wheat crops journal, 2014,34 (1): 137-142. public can be from Hubei Province's agricultural
Cereal crops research institute of the academy of sciences obtains.
Embodiment 1, Qfhb.hbaas-1AS and its discovery for being associated with SNP marker
Material to be tested: WAPS (the Wheat Association Panel of domestic and international 240 parts of establishments of wheat breed (being)
For Scab research) group, it is shown in Table 1.Material therefor is recorded in document: Zhu Zhanwang, Xu Dengan, Cheng Shunhe, waits Chinese
It the identification of wheat breed Stripe Rust Resistance Gene Fhb1 and traces to the source [J] Acta Agronomica Sinica, 2018,44 (4): 473-482. public can be with
It is obtained from Grain Crop Institute of Hubei Academy of Agricultural Sciences.
1 WAPS group kind (being) of table and its source
aFor 90K SNP chip genotyping result；bFor the testing result for marking M-1AS-Fhb；cFHB index under 4 environment
BLUE value (Best linear unbiased estimate), "-" are chip data missing.
One, the discovery of Qfhb.hbaas-1AS and its association SNP marker
1, scab resistance is identified
In 2014~2017 head blight inoculated identification, disease are carried out in Hubei Prov. Acdemy of Agricutural Sciences South Lake experimental plot within continuous 4 years
Opportunistic pathogen bacterial strain is Huang gang 1.Test uses randomized complete-block design, 2 row areas, capable long 1m, line-spacing 0.25m, 2 repetitions.
2014~2016 years, using spray inoculation, the inoculation of disease wheat method was spread using earth's surface in 2017.20d investigation morbidity fringe after inoculation
Several, every fringe spikelet number and sick spikelet number, with formula: head blight disease index (FHB index)=disease incidence × severity calculates
FHBindex, wherein disease incidence is the ratio of morbidity spike number and total spike number, and severity is every fringe disease spikelet number and spikelet number ratio
Average value, with percentage.Then 4 years FHB index BLUE value (Best linear unbiased are calculated
estimate)。
2, genotyping
WAPS group carries out genotyping with 90K SNP chip, and wherein the good SNP of 22922 genotyping results is used for selection
In subsequent analysis, removing miss rate is more than 20%, minimum label of the gene frequency less than 5%, altogether 19803 SNP of residue
For GWAS.
3, GWAS is analyzed
Analysis is associated using the mixed linear model of Tassel v5.0 software kinship (K)+PCA method.When P≤
When 0.001, it is believed that the label is significantly associated with character.
4, Qfhb.hbaas-1AS and its chain SNP marker
Association analysis discovery is located at anti gibberellic disease site on 1AS, significant under 2016 and 2,017 two environment, explains phenotype
Variation 5.6%, representative connective marker are IWB75039, flanking sequence are as follows:
5’-GATTGACAAAGTCACCACATGCACACATGCACCTCAGTCAACAGGTACGT[A/G]ATCTACAGCT
GGACAAATAGCCTACCCATGTCAAGCTCTGCCGGGTGGAC-3 ' (sequence 3, SNP site is in sequence 3 the 51st).
It is with reference to physical location on genome sequence (IWGSC, http://www.wheatgenome.org) in China
10.1Mb
aRepresentative SNP marker,bUnderscore show disease-resistant allele,cChina spring refers to genomic physical position
(IWGSC, http://www.wheatgenome.org),dExplain phenotypic variation.
It is 10.1Mb, polymorphism A/G, the SNP site that SNP site IWB75039, which is located at 1AS chromosome physical location,
Genotype be AA or GG.
Detection and scab resistance identification by above-mentioned SNP site genotype, SNP site genotype is the material 4 of GG
FHB index BLUE value is 33.0% (Fig. 1) lower than the material that genotype is AA under a environment.* indicates two kinds of Genotypes
Between phenotype it is significant in 0.01 level difference.
Two, M-1AS-Fhb marks the design of primer special and its foundation of method
1, genome specific design of primers
With the flanking sequence of SNP IWB75039, EnsemblPlants database (http: //
Plants.ensembl.org it) compares, obtains its homologous sequence, design its 1A chromosome special primer P5039 (sequence 1 and sequence
Column 2) also known as M-1AS-Fhb label, by Beijing, Qing Kexin industry Bioisystech Co., Ltd is synthesized.
P5039F:5 '-GTTTTCAGGGCCTTATCAACTGAAG-3 ' (sequence 1)；
P5039R:5 '-GGTTCGGTTGGTGCTTAATCACT-3 ' (sequence 2).
2, detection method is established
Material to be tested: the WAPS group that domestic and international 240 parts of wheat breed (being) (table 1) is set up.
PCR amplification: the genomic DNA of wheat to be measured is extracted, ddH is added2O dilutes its concentration to 30ng/ μ L as template, adopts
PCR amplification is carried out with the primer pair that primer P5039F and primer P5039R is formed.
Above-mentioned PCR amplification system is as shown in table 3；
The PCR system of 3 primer P5039 of table
PCR program: 95 DEG C of initial denaturation 5min；94 DEG C of denaturation 30s, 60 DEG C of annealing 30s, 72 DEG C of extension 1min 30s, totally 35
A circulation；72 DEG C of extension 5min；4 DEG C of preservations.
With 1.5% agarose gel electrophoresis detect PCR product, if product be 389bp segment, SNPIWB75039
The genotype of point is AA；If the genotype in the site SNPIWB75039 is GG without amplified production.
1-5 shown in Fig. 2 is the kind that IWB75039 genotype is AA, and 6-10 is the kind that IWB75039 genotype is GG；
As can be seen that it is 389bp that genotype, which is AA material amplified production clip size, genotype is GG material without amplified production, is shown
The label can distinguish IWB75039 genotype, and further discriminate between the equipotential base of anti gibberellic disease QTL Qfhb.hbaas-1AS
Cause, label label are named as M-1AS-Fhb (Fig. 2).
Label WAPS crowd surveillance result and SNP IWB75039 genotyping result concordance rate are 96.7%, illustrate the label
It converts successfully.
Therefore, SNP IWB75039 label can be used for assisting detecting wheat to be measured whether anti gibberellic disease, and for resist it is red mould
Sick molecular breeding；The specific method is as follows:
The genotype of SNP site IWB75039 in Wheat volatiles to be measured is detected, is judged as follows:
SNP site IWB75039 genotype is that the scab resistance of GG wheat to be measured is higher than or candidate is higher than IWB75039 base
Because type is AA wheat to be measured.
The method of the genotype of SNP site IWB75039 is as follows in above-mentioned detection Wheat volatiles to be measured:
1) 90K SNP chip is analyzed；
2) PCR is carried out with P5039F and P5039R, if product is the segment of 389bp, wheat SNP site to be measured
The genotype of IWB75039 is AA；If the genotype of wheat SNP site IWB75039 to be measured is GG without PCR product；
3) PCR is carried out with P5039F and P5039R, directly amplified production is sequenced.
Embodiment 2, actual sample detection
Material to be tested: WAPS group kind shown in table 1.
One, scab resistance is identified
With embodiment 1.
Two, M-1AS-Fhb label detection
With M-1AS-Fhb label according to the method in embodiment 1, PCR amplification is carried out to 240 kinds shown in table 1.
If PCR product is the segment of 389bp size, the genotype of wheat SNP site IWB75039 to be measured is AA；If nothing
PCR product, then the genotype of wheat SNP site IWB75039 to be measured is GG.
240 parts of wheat breed label testing results and FHB index average value are as shown in table 1.The result of table 1 is united
Meter analysis, obtains the result of table 4.
4 M-1AS-Fhb different genotype kind scab resistance difference of table
From the above, it can be seen that the scab resistance that SNP site IWB75039 is GG genotype wheat to be measured is higher than or waits
It is AA genotype wheat to be measured that choosing, which is higher than SNP site IWB75039,.
Sequence table
<110>Institute of Crop Science, Chinese Academy of Agricultural Science of Grain Crop Institute of Hubei Academy of Agricultural Sciences
<120>a kind of for detecting the molecular labeling and application method of anti gibberellic disease QTL Qfhb.hbaas-1AS
<160> 3
<170> PatentIn version 3.5
<210> 1
<211> 25
<212> DNA
<213>artificial sequence
<400> 1
gttttcaggg ccttatcaac tgaag 25
<210> 2
<211> 23
<212> DNA
<213>artificial sequence
<400> 2
ggttcggttg gtgcttaatc act 23
<210> 3
<211> 101
<212> DNA
<213>artificial sequence
<220>
<221> misc_feature
<222> (51)..(51)
<223> n is a or g
<400> 3
gattgacaaa gtcaccacat gcacacatgc acctcagtca acaggtacgt natctacagc 60
tggacaaata gcctacccat gtcaagctct gccgggtgga c 101
Claims (9)
1. the substance for detecting the genotype in the site SNP IWB75039 of chromosome of wheat 1AS is identifying or is assisting identification wheat red
Application in mildew resistance；
Or the substance of the genotype in the site SNP IWB75039 of detection chromosome of wheat 1AS is small in preparation identification or auxiliary identification
Application in wheat scab resistance product.
2. detecting the substance of the genotype in the site SNP IWB75039 of chromosome of wheat 1AS in wheat-resistance to scab breeding
Using；
Or, the substance of the genotype in the site SNP IWB75039 of detection chromosome of wheat 1AS is educated preparing wheat-resistance to scab
Application in kind product.
3. application according to claim 1 or 2, it is characterised in that:
The site the SNP IWB75039 is on chromosome of wheat 1AS, and physical location is the site of 10.1Mb；
Or, the genotype in the site the SNP IWB75039 is GG or AA.
4. application according to claim 1 to 3, it is characterised in that:
The substance of the genotype in the site SNP IWB75039 of the detection chromosome of wheat 1AS is following 1) or 2):
1) primer set, primer set single strand dna as shown in sequence 1 in sequence table or derivatives thereof and sequence table
Single strand dna shown in middle sequence 2 or derivatives thereof composition；
2) contain the PCR reagent or kit of the primer set.
5. application according to claim 4, it is characterised in that:
The derivative of single strand dna shown in sequence 1 is by sequence 1 by one or several nucleotide in the sequence table
Replace and with the DNA molecular with the same function of sequence 1；
The derivative of single strand dna shown in sequence 2 is by sequence 2 by one or several nucleotide in the sequence table
Replace and with the DNA molecular with the same function of sequence 2.
6. a kind of product is the SNP IWB75039 of the detection chromosome of wheat 1AS in applying described in claim 1-5
The substance of the genotype in site；
The product has the function of following 1) or 2) at least one:
1) identify or assist identification wheat scab resistance；
2) wheat-resistance to scab breeding.
7. a kind of method of identification or auxiliary identification wheat scab resistance, for the SNP for detecting chromosome of wheat 1AS to be measured
The genotype in the site IWB75039 is GG or AA, and the scab resistance of the wheat to be measured is judged according to genotype；
The genotype in the site SNP IWB75039 is that the scab resistance of the wheat to be measured of GG is higher than the site SNP IWB75039
The genotype of genotype is the wheat to be measured of AA.
8. a kind of method of breeding wheat-resistance to scab, for the gene in the site SNP IWB75039 of detection chromosome of wheat 1AS
Type is GG or AA, and breeding genotype is the wheat to be measured of GG, obtains the wheat of anti gibberellic disease.
9. according to the method described in claim 8, it is characterized by:
The genotype for detecting the site SNP IWB75039 of chromosome of wheat 1AS to be measured is GG or the method for AA is following A) or
B):
A) 90K SNP chip is analyzed；
B PCR amplification) is carried out to the Wheat volatiles DNA to be measured with the primer set in claim 4, detection PCR is produced
Object；
If PCR product is the segment of 389bp size, the genotype of the wheat SNP site IWB75039 to be measured is AA；If nothing
PCR product, then the genotype of the wheat SNP site IWB75039 to be measured is GG.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110878300A (en) * | 2019-11-28 | 2020-03-13 | 中国农业大学 | DNA marker closely linked with wheat 7DL chromosome gibberellic disease resistant gene and application thereof |
| CN111607664A (en) * | 2020-07-13 | 2020-09-01 | 湖北省农业科学院粮食作物研究所 | Application of SNP molecular marker on 1DS chromosome related to wheat stripe rust |
| CN111748645A (en) * | 2020-08-07 | 2020-10-09 | 湖北省农业科学院粮食作物研究所 | Molecular marker for detecting gibberellic disease resistance QTL QFhb.hbaas-5DL and using method thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107338310A (en) * | 2017-07-31 | 2017-11-10 | 中国农业科学院作物科学研究所 | A kind of mark and application method for detecting wheat anti gibberellic disease gene PFT |
| CN108004345A (en) * | 2017-12-20 | 2018-05-08 | 中玉金标记（北京）生物技术股份有限公司 | High throughput detects the method and its kit of wheat anti gibberellic disease Genotyping |
| CN108359739A (en) * | 2018-04-24 | 2018-08-03 | 南京农业大学 | The SNP marker primer of one anti gibberellic disease related gene TaRLK-B and its application |
-
2018
- 2018-11-12 CN CN201811338221.4A patent/CN109207630B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107338310A (en) * | 2017-07-31 | 2017-11-10 | 中国农业科学院作物科学研究所 | A kind of mark and application method for detecting wheat anti gibberellic disease gene PFT |
| CN108004345A (en) * | 2017-12-20 | 2018-05-08 | 中玉金标记（北京）生物技术股份有限公司 | High throughput detects the method and its kit of wheat anti gibberellic disease Genotyping |
| CN108359739A (en) * | 2018-04-24 | 2018-08-03 | 南京农业大学 | The SNP marker primer of one anti gibberellic disease related gene TaRLK-B and its application |
Non-Patent Citations (4)
| Title |
|---|
| JIN SUN等: "Mapping Four Quantitative Trait Loci Associated with Type I Fusarium Head Blight Resistance in Winter Wheat ‘INW0412’", 《CROP SCIENCE》 * |
| SHICHEN WANG等: "Characterization of polyploid wheat genomic diversity using a high-density 90 000 single nucleotidepolymorphism array", 《PLANT BIOTECHNOLOGY JOURNAL》 * |
| XIN YI等: "genetic analysis of Fusarium head blight resistance in cimmyt bread wheat line C615 using traditional and conditional QTL mapping", 《FRONT PLANT SCI》 * |
| 徐晓武: "小麦抗赤霉病侵入基因Fhb5的精细定位", 《中国优秀硕士学位论文全文数据库 农业科技辑》 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110878300A (en) * | 2019-11-28 | 2020-03-13 | 中国农业大学 | DNA marker closely linked with wheat 7DL chromosome gibberellic disease resistant gene and application thereof |
| CN110878300B (en) * | 2019-11-28 | 2022-09-13 | 中国农业大学 | DNA marker closely linked with wheat 7DL chromosome gibberellic disease resistant gene and application thereof |
| CN111607664A (en) * | 2020-07-13 | 2020-09-01 | 湖北省农业科学院粮食作物研究所 | Application of SNP molecular marker on 1DS chromosome related to wheat stripe rust |
| CN111607664B (en) * | 2020-07-13 | 2023-08-08 | 湖北省农业科学院粮食作物研究所 | Application of SNP molecular marker on 1DS chromosome related to wheat stripe rust |
| CN111748645A (en) * | 2020-08-07 | 2020-10-09 | 湖北省农业科学院粮食作物研究所 | Molecular marker for detecting gibberellic disease resistance QTL QFhb.hbaas-5DL and using method thereof |
| CN111748645B (en) * | 2020-08-07 | 2023-04-25 | 湖北省农业科学院粮食作物研究所 | Molecular marker for detecting scab resistance QTL QFhb.hbaas-5DL and application method thereof |
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Application publication date: 20190115 Assignee: Wuhan Xinbai Bioengineering Co.,Ltd. Assignor: HUBEI ACADEMY OF AGRICULTURAL SCIENCES INSTITUTE OF FOOD CROPS Contract record no.: X2022980004755 Denomination of invention: A QTL qfhb for detecting scab resistance Molecular marker of hbaas-1as and its application Granted publication date: 20211224 License type: Common License Record date: 20220425 |
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Application publication date: 20190115 Assignee: Hubei huichuzhi Biotechnology Co.,Ltd. Assignor: HUBEI ACADEMY OF AGRICULTURAL SCIENCES INSTITUTE OF FOOD CROPS Contract record no.: X2022420000059 Denomination of invention: A molecular marker and application method for detecting Scab Resistant QTL qfhb.hbaas-1as Granted publication date: 20211224 License type: Common License Record date: 20220714 |
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Application publication date: 20190115 Assignee: Jingzhou jiazhiyuan Biotechnology Co.,Ltd. Assignor: HUBEI ACADEMY OF AGRICULTURAL SCIENCES INSTITUTE OF FOOD CROPS Contract record no.: X2022420000061 Denomination of invention: A molecular marker and application method for detecting Scab Resistant QTL qfhb.hbaas-1as Granted publication date: 20211224 License type: Common License Record date: 20220714 Application publication date: 20190115 Assignee: Hubei Meitian Biotechnology Co.,Ltd. Assignor: HUBEI ACADEMY OF AGRICULTURAL SCIENCES INSTITUTE OF FOOD CROPS Contract record no.: X2022420000060 Denomination of invention: A molecular marker and application method for detecting Scab Resistant QTL qfhb.hbaas-1as Granted publication date: 20211224 License type: Common License Record date: 20220714 Application publication date: 20190115 Assignee: Jingzhou Changnong Agricultural Technology Co.,Ltd. Assignor: HUBEI ACADEMY OF AGRICULTURAL SCIENCES INSTITUTE OF FOOD CROPS Contract record no.: X2022420000062 Denomination of invention: A molecular marker and application method for detecting Scab Resistant QTL qfhb.hbaas-1as Granted publication date: 20211224 License type: Common License Record date: 20220714 Application publication date: 20190115 Assignee: Hubei Chuyu Seed Industry Co.,Ltd. Assignor: HUBEI ACADEMY OF AGRICULTURAL SCIENCES INSTITUTE OF FOOD CROPS Contract record no.: X2022420000063 Denomination of invention: A molecular marker and application method for detecting Scab Resistant QTL qfhb.hbaas-1as Granted publication date: 20211224 License type: Common License Record date: 20220714 |
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