CN106591489A - Rice grain length gene GW7 molecular marker and special primer sequences thereof - Google Patents
Rice grain length gene GW7 molecular marker and special primer sequences thereof Download PDFInfo
- Publication number
- CN106591489A CN106591489A CN201710113391.1A CN201710113391A CN106591489A CN 106591489 A CN106591489 A CN 106591489A CN 201710113391 A CN201710113391 A CN 201710113391A CN 106591489 A CN106591489 A CN 106591489A
- Authority
- CN
- China
- Prior art keywords
- grain length
- paddy rice
- length gene
- molecular labeling
- rice grain
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/13—Plant traits
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Immunology (AREA)
- Mycology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Botany (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention belongs to the technical field of molecular biology, and provides a rice grain length gene GW7 molecular marker and special primer sequences thereof; primers GW7DelF and GW7DelR are synthesized through design; DNAs of different rice are amplified in a PCR system by the primers, and whether the amplified products contain GW7 allelic genes is detected by electrophoresis. The molecular marker can fast, accurately and directly identify whether rice germplasms or breeding populations contain the GW7 allelic genes, has simple use method, can save a lot of time, materials and human resources, and reduces the cost of breeding.
Description
Technical field
The invention belongs to technical field of molecular biology, is related to a kind of paddy rice grain length geneGW7Molecular labeling and its specially
Use primer sequence.
Background technology
Paddy rice is in the world more than the important component part of half population food.Rice grain shape be the important yield of paddy rice and
Correlation of attributes proterties, mainly including grain length, grain is wide, grain is thick, grain is heavy and length-width ratio, due to the outward appearance product of Grain shape traits and paddy rice
Matter, processing quality, boiling and Cooking Quality etc. have close relationship, thus rice grain shape not only affects rice yield, and
And the quality of impact rice, and the yield and quality formation to paddy rice all has important function.The seed mass of 1000 kernel of paddy rice is main
Determine that is, oarse-grained rice varieties grain yield is high by grain width and grain thickness, but the importance in exterior quality
Shape --- chalkness ratio is wide with grain and grain is thick in significantly correlated.Chalk is in vain opaque part in rice endosperm, and the part endosperm is thin
The starch accumulation of intracellular is insufficient.It is fluffy hollow after the high rice boiling of chalkiness degree, have a strong impact on quality.Therefore, normal conditions
The high general inferior quality of rice varieties of lower yield, and the rice varieties of quality better often seed is elongated and yield is relatively low, therefore
The formation of yield and quality is typical negative correlation, and the raising of final quality and yield remains a challenge.Paddy riceGW7Gene
Secondary structure --- the homologous sequence of tonneau1 of one recruitment albumen of coding, this is coded ofGW7With anthropocentric body
The C-terminal motif of PROTEIN C AP350 is similar.The generation of elongated seed withGW7The rise of expression is relevant, is primarily due to indulge
To fissional increase and the reduction of lateral cell division.Quantitative analysis NIL-GW7（Safe rich A）And NIL-gw7(Magnificent Jing Xian
74) two NIL plantGW7The each allele change in site and the difference of Seed shape, NIL-GW7（Safe rich A）Seed
Profile compares NIL-gw7(magnificent Jing Xian 74) is more elongated.Sequence analysis is displayed in safe rich A（Long grain）With magnificent Jing Xian 74（Short grain）InGW7There is the difference of 18 SNP and 9 disappearances in the promoter region of gene.
The molecular markers development of particle shape gene has at presentGS3Gene andGW8Gene.GS3The specific molecular marker of gene
There are two types：It is a kind of to mark for sf28, it is made up of positive and negative two primers, after PCR amplifications, need restriction enzyme digestion to digest
Overnight, then electrophoresis detection is carried out, high cost, time-consuming, is not suitable with high-throughout molecular marker assisted selection.Another kind is M-
GS3 is marked, according togs3Site SNP mutation（C-A）, devising can track the dominant molecular labeling M-gs3 of long grain allele
（It is made up of three primers）, expanded by PCR, directly can detect in paddy rice sample whether contain with 1.2% agarose gel electrophoresis
The mutational site of TGA.The method can be identified fast and effectively in paddy rice samplegs3Long grain allele.GeneGW8Molecule
Mark is the point mutation according to the Indel sequence variations of one 10 bp of promoter region and 2 missense mutation of the 3rd extron
Sequencing primer is separately designed, then purpose band is obtained by PCR amplifications, digestion and electrophoresis.
There is no at present and be directed to particle shape geneGW7The molecular labeling of exploitation, in geneGW7Identification on also without direct
Effective method.GeneGW7Play an important role in rice grain shape change, due to identifying without directly effective method
GW7 genes and its allelic variation in each sample, cannot determine the correspondence imported in kind during grain length proterties seed selection
The genetic mutation of shape, and traditional linkage inheritance Marker-assisted selection process complexity, time length and workload are big, increased seed selection
Difficulty.
The content of the invention
For above technical problem, the invention provides a kind of paddy rice grain length geneGW7Molecular labeling and its special draw
Whether thing sequence, directly effectively contain paddy rice grain length in identification paddy riceGW7Allele, can save the plenty of time, manpower,
Material resources, are easy to operation.
In this regard, the technical scheme is that：A kind of paddy rice grain length geneGW7Molecular labeling primer special sequence,
The paddy rice grain length geneGW7Allele molecular labeling primer：
GW7DelF：TGACACATATCTCATCATACCATCA；
GW7DelR：AGAGCAGATGCCACAGGAAG.
Present invention also offers a kind of identification paddy rice grain length geneGW7Molecular labeling, carry out molecule using following steps
Mark：
Step S1：Extract the genomic DNA of paddy rice；
Step S2：PCR is expanded：Oryza sativa genomic dna with step S1 as template, described GW7DelF and GW7DelR primers
In being added to PCR reaction systems, amplification is carried out to described oryza sativa genomic dna and obtains amplified production；
Step S3：By pcr amplification product in the Ago-Gel that mass percent concentration is 1.2% electrophoresis, ethidium bromide dye
Color, acquisition electrophoretogram of then observing under uviol lamp and take pictures；
Step S4：It is analyzed according to electrophoretogram.
Further, then it is containing grain length if amplified band when carrying out electrophoresis map analysisGW7Allele；If not yet
There is amplified band, be then without grain lengthGW7Allele.
Further, the PCR reaction systems are the system of 20 ul：10 × Buffer (Mg of 20mM/L of 2.0ul2+),
The dNTP of 2.0ul（10mM/L）, the ul of GW7DelF primers 1.0 of 4umol/L, the ul of GW7DelR primers 1.0 of 4umol/L, 0.2
The Taq enzyme (2U/L) of ul, the template DNA (40ng/ul) of 2.0 ul, the ddH of 13.8 ul2O。
Further, the response procedures of the PCR amplifications are：94 DEG C of min of denaturation 5；94 DEG C of s of denaturation 30,58 DEG C move back
30 s of fire, 72 DEG C of 45 s of extension, circulate 35 times；Then amplified production is obtained after 72 DEG C of 10 min of extension.
Compared with prior art, beneficial effects of the present invention are：
1st, to grain length geneGW7Molecular markers for identification is carried out, to the identification of paddy rice grain length more directly effectively.
2nd, using method is simple, is easy to operation, can save plenty of time, human and material resources, reduces breeding cost.
Description of the drawings
Fig. 1 is the agarose gel electrophoresis figure of 24 parental rice kind molecular labelings of present invention detection,
In figure, 1-112B；2-IR1552；The safe rich B of 3-；4-138B；5- wins IIB；6-838R；7- polyphylys one；The rich B of 8- five；9-
128R；10- R774；11- osmanthus 99；12- Y58S；13- spy B；14- R795；15-R204；16- R582；17- R463；18-
Yanhui 559；19- IRBB7；20- Fuhui 838s；21- Shan B；22- surveys 253；23- depth 08S；24- China accounts for；
Fig. 2 is the agarose gel electrophoresis figure of 24 Rice Progenies molecular labelings of present invention detection.
Specific embodiment
Technical solution of the present invention is further described below with reference to drawings and Examples.
Embodiment 1
A kind of paddy rice grain length geneGW7Molecular labeling primer special sequence, the paddy rice grain length geneGW7Allele
Molecular labeling primer：
GW7DelF：TGACACATATCTCATCATACCATCA；
GW7DelR：AGAGCAGATGCCACAGGAAG.
Paddy rice grain length geneGW7Molecular markers for identification specific implementation step it is as follows：
(1) oryza sativa genomic dna is extracted
The blade of 24 parts of parental rice kinds is taken, by CTAB extraction methods（Cetyl trimethylammonium bromide method）Obtain paddy rice
Genomic DNA.
(2) PCR amplifications
PCR reaction systems are the system of 20 μ L：10 × Buffer (Mg of 20mM/L of 2.0ul2+), the dNTP of 2.0ul
（10mM/L）, the ul of GW7DelF primers 1.0 of 4umol/L, the ul of GW7DelR primers 1.0 of 4umol/L, the Taq enzyme of 0.2 ul
(2U/L), the template DNA (40ng/ul) of 2.0 ul, the ddH of 13.8 ul2O。
PCR amplification response procedures be：94 DEG C of min of denaturation 5；94 DEG C of s of denaturation 30,58 DEG C of 30 s of annealing, 72 DEG C
Extend 45 s, circulate 35 times；Then amplified production is obtained after 72 DEG C of 10 min of extension.
(3) by pcr amplification product in the Ago-Gel that mass percent concentration is 1.2% electrophoresis, ethidium bromide dye
Color, acquisition electrophoretogram of then observing under uviol lamp and take pictures.
(4) result and analysis
The electrophoretogram of the molecular labeling of 24 parental rice kinds refers to Fig. 1, the grain length and genotype of 24 parental rice kinds
Refer to table 1.
As shown in figure 1, in 24 parents detection, being numbered 3 and 15 swimming lane has amplified band, be containingGW7Allele
Kind, and other be withoutGW7Particle shape phenotypic evaluation result is basically identical in the kind of allele, with table 1.
1 24 rice varieties of table and its grain length phenotype and genotype
| Numbering | Kind name | Grain length（mm） | Genotype | Numbering | Variety name | Grain length（mm） | Genotype |
| 1 | 112B | 9.03 | - | 13 | Special B | 8.32 | - |
| 2 | IR1552 | 8.73 | - | 14 | R795 | 9.24 | - |
| 3 | Safe rich B | 11.8 | + | 15 | R204 | 13.1 | + |
| 4 | 138B | 8.90 | - | 16 | R582 | 8.94 | - |
| 5 | Rich II B | 8.03 | - | 17 | R463 | 9.72 | - |
| 6 | 838R | 9.80 | - | 18 | Yanhui 559 | 8.41 | - |
| 7 | Polyphyly one | 10.2 | - | 19 | IRBB7 | 9.33 | - |
| 8 | Five rich B | 8.45 | - | 20 | Fuhui 838 | 10.3 | - |
| 9 | 128R | 8.83 | - | 21 | Shan B | 8.85 | - |
| 10 | R774 | 8.83 | - | 22 | Survey 253 | 9.45 | - |
| 11 | Osmanthus 99 | 10.2 | - | 23 | Deep 08S | 8.86 | - |
| 12 | Y58S | 9.25 | - | 24 | China accounts for | 9.98 | - |
Embodiment 2
(1) rice material
Using the medium Y58S two-line sterile lines of particle shape condition and containing long grain geneGW7Safe rich B hybridization and backcross improvement not
Educate the grain length for being.The primer sequence of design is can use to detect resulting individual plant in first backcross generation, after taking 24 hybridization
Tested for individuality.
(2) amplification experiment
Oryza sativa genomic dna extraction, the experiment condition of PCR amplifications, step are same as Example 1.By pcr amplification product in quality
Percent concentration be 1.2% Ago-Gel in electrophoresis, ethidium bromide staining, under uviol lamp observation take pictures.
(3) result and analysis
Can be visually seen from Fig. 2GW7Distribution situation of the allele in each filial generation individuality, according to the DNA cloning
As a result purpose individual plant is selected, is improved in objective trait and is selected rate.
Above content is to combine specific preferred embodiment further description made for the present invention, it is impossible to assert
The present invention be embodied as be confined to these explanations.For general technical staff of the technical field of the invention,
On the premise of without departing from present inventive concept, some simple deduction or replace can also be made, should all be considered as belonging to the present invention's
Protection domain.
SEQUENCE LISTING
<110>Gaungxi Crop Hereditary Improve Biotechnology Key Open Laboratory
Rice research institute of Guangxi Autonomous Region Academy of Agricultural Sciences
<120>The molecular labeling and its primer special sequence of paddy rice grain length gene GW7
<130>
<160> 2
<170> PatentIn version 3.3
<210> 1
<211> 25
<212> DNA
<213>Artificial sequence
<400> 1
tgacacatat ctcatcatac catca 25
<210> 2
<211> 20
<212> DNA
<213>Artificial sequence
<400> 2
agagcagatg ccacaggaag 20
Claims (5)
1. a kind of paddy rice grain length geneGW7Molecular labeling primer special sequence, it is characterised in that the paddy rice grain length geneGW7Allele molecular labeling primer：
GW7DelF：TGACACATATCTCATCATACCATCA；
GW7DelR：AGAGCAGATGCCACAGGAAG.
2. a kind of molecular labeling of paddy rice grain length gene GW7, it is characterised in that carry out molecular labeling using following steps：
Step S1：Extract the genomic DNA of paddy rice；
Step S2：PCR is expanded：Oryza sativa genomic dna with step S1 as template, by the GW7DelF described in claim 1 and
GW7DelR primers are added in PCR reaction systems, are carried out amplification to described oryza sativa genomic dna and are obtained amplified production；
Step S3：By pcr amplification product in the Ago-Gel that mass percent concentration is 1.2% electrophoresis, ethidium bromide dye
Color, acquisition electrophoretogram of then observing under uviol lamp and take pictures；
Step S4：It is analyzed according to electrophoretogram.
3. paddy rice grain length gene according to claim 2GW7Molecular labeling, it is characterised in that：Carry out electrophoresis map analysis
When, then it is containing grain length if amplified bandGW7Allele；If being without grain length without amplified bandGW7Deng
Position gene.
4. paddy rice grain length gene according to claim 3GW7Molecular labeling, it is characterised in that the PCR reaction systems
For the system of 20 ul：10 × Buffer (Mg of 20mM/L of 2.0ul2+), the dNTP of 2.0ul（10mM/L）, 4umol/L's
The Taq enzyme (2U/L) of the ul of GW7DelR primers 1.0 of the ul of GW7DelF primers 1.0,4umol/L, 0.2 ul, the template of 2.0 ul
DNA (40ng/ul), the ddH of 13.8 ul2O。
5. paddy rice grain length gene according to claim 4GW7Molecular labeling, it is characterised in that：It is anti-that the PCR is expanded
The program is answered to be：94 DEG C of min of denaturation 5；94 DEG C of s of denaturation 30,58 DEG C of 30 s of annealing, 72 DEG C of 45 s of extension, circulate 35 times；So
Obtain amplified production after 72 DEG C of 10 min of extension afterwards.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710113391.1A CN106591489B (en) | 2017-02-28 | 2017-02-28 | Molecular marker of rice grain length gene GW7 and special primer sequence thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710113391.1A CN106591489B (en) | 2017-02-28 | 2017-02-28 | Molecular marker of rice grain length gene GW7 and special primer sequence thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106591489A true CN106591489A (en) | 2017-04-26 |
| CN106591489B CN106591489B (en) | 2021-01-26 |
Family
ID=58588178
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710113391.1A Active CN106591489B (en) | 2017-02-28 | 2017-02-28 | Molecular marker of rice grain length gene GW7 and special primer sequence thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106591489B (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110592079A (en) * | 2019-09-23 | 2019-12-20 | 湖北省农业科学院粮食作物研究所 | Rice long and thin granule gene SLG7 molecular marker primer and application thereof |
| CN112210616A (en) * | 2019-07-12 | 2021-01-12 | 南京农业大学 | InDel molecular marker primer related to length traits of rice grains and application thereof |
| CN112609017A (en) * | 2020-12-08 | 2021-04-06 | 浙江大学 | Molecular marker for detecting rice grain shape, corresponding gene and application |
| CN113151575A (en) * | 2021-06-04 | 2021-07-23 | 中国水稻研究所 | InDel molecular marker GW6a-InDel of rice grain shape major QTL and detection primer and application thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104745599A (en) * | 2013-12-31 | 2015-07-01 | 华中农业大学 | Rice granule shape gene qSS7 as well as preparation method and application |
| CN104928286A (en) * | 2014-12-24 | 2015-09-23 | 中国水稻研究所 | Molecular marker of dominant grain length gene of rice |
| CN104946661A (en) * | 2014-12-24 | 2015-09-30 | 中国水稻研究所 | Rice grain shape regulatory gene GL7 and application thereof |
| CN105985965A (en) * | 2015-02-06 | 2016-10-05 | 中国科学院遗传与发育生物学研究所 | Gene GW7 for controlling grain shape, exterior quality and yield of rice and applications of gene GW7 |
-
2017
- 2017-02-28 CN CN201710113391.1A patent/CN106591489B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104745599A (en) * | 2013-12-31 | 2015-07-01 | 华中农业大学 | Rice granule shape gene qSS7 as well as preparation method and application |
| CN104928286A (en) * | 2014-12-24 | 2015-09-23 | 中国水稻研究所 | Molecular marker of dominant grain length gene of rice |
| CN104946661A (en) * | 2014-12-24 | 2015-09-30 | 中国水稻研究所 | Rice grain shape regulatory gene GL7 and application thereof |
| CN105985965A (en) * | 2015-02-06 | 2016-10-05 | 中国科学院遗传与发育生物学研究所 | Gene GW7 for controlling grain shape, exterior quality and yield of rice and applications of gene GW7 |
Non-Patent Citations (2)
| Title |
|---|
| LIZHEN SI等: "OsSPL13 controls grain size in cultivated rice", 《NATURE GENETICS》 * |
| SHAOKUI WANG等: "The OsSPL16-GW7 regulatory module determines grain shape and simultaneously improves rice yield and grain quality", 《NATURE GENETICS》 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112210616A (en) * | 2019-07-12 | 2021-01-12 | 南京农业大学 | InDel molecular marker primer related to length traits of rice grains and application thereof |
| CN112210616B (en) * | 2019-07-12 | 2022-03-01 | 南京农业大学 | InDel molecular marker primer related to length traits of rice grains and application thereof |
| CN110592079A (en) * | 2019-09-23 | 2019-12-20 | 湖北省农业科学院粮食作物研究所 | Rice long and thin granule gene SLG7 molecular marker primer and application thereof |
| CN112609017A (en) * | 2020-12-08 | 2021-04-06 | 浙江大学 | Molecular marker for detecting rice grain shape, corresponding gene and application |
| CN113151575A (en) * | 2021-06-04 | 2021-07-23 | 中国水稻研究所 | InDel molecular marker GW6a-InDel of rice grain shape major QTL and detection primer and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN106591489B (en) | 2021-01-26 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US11041167B2 (en) | Methods and compositions for selecting soybean plants resistant to Phytophthora root rot | |
| CN107090468B (en) | Methods and compositions for selecting soybean plants resistant to meloidogyne incognita | |
| CN102098909A (en) | A method to identify asian soybean rust resistance quantitative trait loci in soybean and compositions thereof | |
| CN106011287B (en) | The molecular labeling in the site rice bacterial leaf streak major gene resistance BLS1 and its application | |
| CN106591489A (en) | Rice grain length gene GW7 molecular marker and special primer sequences thereof | |
| US20180371483A1 (en) | Molecular markers for low palmitic acid content in sunflower (helianthus annus), and methods of using the same | |
| CN106244678B (en) | The molecular labeling of rice bacterial leaf streak major gene resistance BLS1 and its application | |
| CN108977440A (en) | A kind of molecular labeling and application method for 895 stripe rust resisting QTL of wheat in detecting | |
| CN109688805B (en) | Method for producing gray leaf spot resistant maize | |
| CN104672315B (en) | Control gene and to Cucumber Roll palpus character related SNP marker of the cucumber without tendril character | |
| CN108285927A (en) | Molecular labeling Caps7 and its application in a kind of gene of rice Cd accumulation related gene OsHMA3 | |
| CN109628628B (en) | Development and application of SNP (single nucleotide polymorphism) marker of rice blast resistance gene Pi2 | |
| CN110512025A (en) | A kind of molecular labeling and its application with powdery mildew resistance gene in wheat PmJM23 close linkage | |
| Beser et al. | Marker-assisted introgression of a broad-spectrum resistance gene, Pi40 improved blast resistance of two elite rice (Oryza sativa L.) cultivars of Turkey | |
| CN104328119A (en) | Microsatellite molecular marker related to growth character of megalobrama amblycephala and application of molecular marker | |
| CN102304587A (en) | Method for rapidly identifying erect panicle of rice | |
| CN102747070A (en) | Two CAPs markers tightly linked with muskmelon anti-powdery mildew gene Pm-AN and its application method | |
| CN104561353B (en) | InDel marker in close linkage with cabbage fertility as well as detection method and application of InDel marker | |
| CN112301142A (en) | Leymus speetanus anti-powdery mildew gene Pm12 molecular marker and application thereof | |
| CN109797238A (en) | Two kinds of molecular labelings and application thereof for gummy stem blight of melon Resistance Identification based on the exploitation of anti didymella linked gene | |
| CN108950049A (en) | The SNP marker and its application of bacterial spot of tomato close linkage | |
| CN106701917A (en) | Special primer for identifying gummy stem blight resistance of muskmelon and molecular marking method | |
| CN109735650A (en) | Four kinds of Gummy Stem Blight Resistance in Melon molecular labelings and purposes based on single nucleotide polymorphism | |
| CN109055594A (en) | A kind of molecular labeling and application method for 895 anti gibberellic disease QTL of wheat in detecting | |
| CN116200528B (en) | SNP molecular marker linked with wheat stripe rust resistance gene QYr.sicau. -2BL and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |