CN109200624A - A method of step by step arithmetic Semen Coicis water extract and Semen Coicis alcohol extract from Semen Coicis - Google Patents
A method of step by step arithmetic Semen Coicis water extract and Semen Coicis alcohol extract from Semen Coicis Download PDFInfo
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- CN109200624A CN109200624A CN201811082091.2A CN201811082091A CN109200624A CN 109200624 A CN109200624 A CN 109200624A CN 201811082091 A CN201811082091 A CN 201811082091A CN 109200624 A CN109200624 A CN 109200624A
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- semen coicis
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D11/00—Solvent extraction
- B01D11/02—Solvent extraction of solids
- B01D11/028—Flow sheets
- B01D11/0284—Multistage extraction
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D11/00—Solvent extraction
- B01D11/02—Solvent extraction of solids
- B01D11/0288—Applications, solvents
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Abstract
The method of the present invention relates to a kind of from Semen Coicis step by step arithmetic Semen Coicis water extract and Semen Coicis alcohol extract, belongs to active substance of plant extractive technique field.First by Semen Coicis degreasing, degreasing coixlacrymajobi powder is obtained, water is then carried out and mentions, obtain Semen Coicis water extract, finally carry out alcohol extracting, obtain Semen Coicis alcohol extract.The present invention has separated the different component of Semen Coicis by step by step arithmetic, facilitates the functional activity substance of research Semen Coicis, and wrap up raw material using gauze, substantially reduces impurity content in extract, simplify extraction operation, reduce the loss of raw material；The present invention is enriched the active material in Semen Coicis by step by step arithmetic, improves the utility value of Semen Coicis, and wherein water extract is rich in soluble protein and sugar matter, and alcohol extract is rich in polyphenol and flavones, can be using extract as food or the functional adding ingredient of cosmetics.
Description
Technical field
The method of the present invention relates to a kind of from Semen Coicis step by step arithmetic Semen Coicis water extract and Semen Coicis alcohol extract, it is living to belong to plant
Property substance extractive technique field.
Background technique
Semen Coicis also known as semen coicis, adlay are the dry mature kernals of non-graminaceous plant coix lacryma-jobi.Semen Coicis main product Fujian, river
The ground such as Soviet Union, Hebei, Liaoning, Guangxi, Guizhou, natural resources very abundant.Semen Coicis is full of nutrition, easy to digest, is known as " the world
The king of gramineae plant ".Studies have shown that Semen Coicis has, the effects of hypoglycemic, blood pressure lowering, antitumor, anti-inflammatory.Semen Coicis has
The pharmacological activity such as swelling diuretic, hypoglycemic, blood pressure lowering, antitumor, anti-inflammatory, the intake of Semen Coicis can promote the health of human body, mention
High function of human body.These physiological functions of Semen Coicis mainly have benefited from the phytochemicals abundant contained by it: Polyphenols, flavones
Class, vitamin, cellulose, minerals etc., in addition, Semen Coicis also contains functional amino and protein, functional grease also has
Higher content.
Functional components class volume is big in Semen Coicis, and the lipids such as coixenolide included have anti-cancer anti-inflammation effect, Semen Coicis
Polyphenol has antitumor and antioxidation, and Semen Coicis amino acid composition is balanced, nutritive value height, etc..In the prior art, it closes
The extraction for being substantially Semen Coicis polyphenol of note, and seldom about the extraction of other function ingredient research, therefore, the prior art pair
The extraction and application of Semen Coicis is simultaneously insufficient.Therefore, step by step arithmetic is carried out to Semen Coicis different function component, separated utilize gets a good eye valence
Value, can improve Semen Coicis utilization rate and improve the functional importance of Semen Coicis product.
Summary of the invention
Based on this, the present invention provides the method for a kind of step by step arithmetic Semen Coicis water extract and Semen Coicis alcohol extract from Semen Coicis, this
Invention obtains the extract of two kinds of different function ingredients by solvent step by step arithmetic of n-hexane, water and ethanol solution, improves the heart of a lotus seed
The utility value of rice, solves problems of the prior art.
Technical solution
A method of step by step arithmetic Semen Coicis water extract and Semen Coicis alcohol extract from Semen Coicis include the following steps:
(1) it dries after cleaning Semen Coicis, then crushes, cross 60-100 mesh, coixlacrymajobi powder is obtained, by coixlacrymajobi powder gauze packet
After wrapping up in, n-hexane is added, then shakes degreasing 1-2h in a water bath, takes out gauze packet, obtains degreasing coixlacrymajobi powder, dry standby
With；
(2) the gauze packet equipped with degreasing coixlacrymajobi powder after drying is taken, is added in distilled water, is soaked in 65~70 DEG C of water-bath
Mention, then take out gauze be responsible for a task until it is completed it is dry after it is stand-by, supernatant is collected after centrifugation in extracting solution, after supernatant removes solvent, then is freezed
It is dry, obtain Semen Coicis water extract；
(3) the gauze packet in step (2) is taken, 50% ethanol solution is added, extracts in 65~70 DEG C of water-bath, then goes
Fall gauze packet, supernatant is collected after centrifugation in extracting solution, after supernatant removes solvent, then is freeze-dried, obtains Semen Coicis alcohol extract.
Further, in step (1), the solid-liquid ratio of coixlacrymajobi powder and n-hexane is 1:4.
Further, in step (1), bath temperature is 55~60 DEG C.
Further, in step (2), the solid-liquid ratio of degreasing coixlacrymajobi powder and distilled water is 1:8 after drying.
Further, in step (2), centrifugation number is centrifugal rotational speed 6000r/min more than twice, and every centrifugation time is
10min。
Further, in step (2) and (3), extraction time 4-5h.
Further, in step (3), solid-liquid ratio 1:8.
Further, in step (3), centrifugation number is centrifugal rotational speed 6000r/min more than twice, and every centrifugation time is
10min。
The beneficial effects of the present invention are: the present invention has separated the different component of Semen Coicis by step by step arithmetic, it is convenient better
Study the functional activity substance of Semen Coicis；The present invention wraps up raw material using gauze, substantially reduces impurity content in extract, and aobvious
Work simplifies extraction operation, and reduces the loss of raw material；The present invention is enriched the active material in Semen Coicis by step by step arithmetic,
The utility value of Semen Coicis is improved, wherein water extract is rich in soluble protein and sugar matter, and alcohol extract is rich in polyphenol and flavones, can be with
It can be used as the anti-senescence function of facial mask for extract as food or the functional adding ingredient of cosmetics, such as Semen Coicis alcohol extract
Ingredient.
Specific embodiment
The present invention is described in detail With reference to embodiment.
Embodiment 1
A method of step by step arithmetic Semen Coicis water extract and Semen Coicis alcohol extract from Semen Coicis include the following steps:
(1) it dries after cleaning Semen Coicis, then crushes, cross 60 meshes, obtain coixlacrymajobi powder, coixlacrymajobi powder is wrapped up with gauze
Afterwards, n-hexane is added, then solid-liquid ratio 1:4 shakes degreasing 1-2h in 55~60 DEG C of water-baths, take out gauze packet, obtain degreasing
Coixlacrymajobi powder, it is spare after dry；
(2) the gauze packet equipped with degreasing coixlacrymajobi powder after drying is taken, is added in distilled water, solid-liquid ratio 1:8, at 65~70 DEG C
Water-bath in extract 4-5h, then take out gauze be responsible for a task until it is completed it is dry after it is stand-by, by extracting solution centrifugation (centrifugal rotational speed 6000r/min, from
The heart time is 10min) supernatant is collected afterwards, it repeated centrifugation 2 times, after supernatant rotary evaporation removes solvent, then is freeze-dried, obtains
To Semen Coicis water extract；
(3) the gauze packet in step (2) is taken, 50% ethanol solution, solid-liquid ratio 1:8, in 65~70 DEG C of water-bath is added
4-5h is extracted, gauze packet is then removed, extracting solution centrifugation (centrifugal rotational speed 6000r/min, centrifugation time 10min) is received afterwards
Collect supernatant, repeated centrifugation 2 times, after supernatant removes solvent, then is freeze-dried, obtains Semen Coicis alcohol extract.
Semen Coicis water extract composition measurement:
Total Soluble Sugar, protein, amino acid content is carried out to Semen Coicis water extract made from embodiment 1 to analyze, point
Analysis method and result are as follows:
(1) amino acid forms
It is analyzed by automatic amino acid analyzer, the results are shown in Table 1:
Table 1
(2) Soluble adhesion molecule
Anthrone reagent 4.0ml is added immediately in the glucose solution of various concentration, is dipped in boiling water bath together after adding,
It accurately boils 10 minutes and takes out, it is cooling with flowing water, 10 minutes are placed at room temperature for, the colorimetric under 620nm wavelength.Contained with standard glucose
Amount makees abscissa, makees standard curve, standard deviation R as ordinate with light absorption value2It is 0.9914, calibration curve equation y=
0.0027x+0.0541.Extract is diluted into certain multiple, drawing 1mL, 4.0ml is added in Boiling tube in diluted extracting solution
Anthrone reagent, with a same standard curve making of operation.Colorimetric wavelength 620nm records absorbance, grape is found on standard curve
The content of sugar calculates the Soluble adhesion molecule average value that parallel test obtains three times, and measuring Soluble adhesion molecule is 28.4
± 0.5%.
(3) protein content
The first method kelvin referring to the measurement of Protein in Food in GB-5009-5-2016 national food safety standard is fixed
Nitrogen method, measuring protein content using Protein Analyzer is 7.86%.
Semen Coicis alcohol extract activity substance measuring:
(1) polyphenol content
Then the galla turcica acidity scale product for weighing 0.1g take again with distilled water constant volume in the volumetric flask of 100ml from volumetric flask
In volumetric flask of the 5ml with distilled water constant volume to 50ml.Not eating for 0ml, 0.2ml, 0.4ml, 0.6ml, 0.8ml, 1.0ml is taken respectively
5 times of diluted forint phenol reagents that 1ml is added in sub- acid standard solution (0.1mg/ml) mix, and are added 2ml's after reacting 5min
15%Na2CO3 solution is protected from light 2h with distilled water constant volume to 10ml, measures the absorbance under 760nm.Using concentration as abscissa,
Absorbance is that ordinate makees standard curve, obtains regression equation y=41.76x+0.1, R2=0.991.Take sample molten with 50% ethyl alcohol
Solution is diluted to 1mg/mL, crosses the filter membrane of 0.45um, and after diluting 10 times with distilled water, 5 times of diluted Folin-Phenol examinations of 1ml are added
Agent mixes, and reacts the 15%Na2CO3 solution of addition 2ml after 5min, with distilled water constant volume to 10ml, is protected from light 2h, measures 760nm
Under absorbance.Polyphenol content is calculated according to standard curve, the results are shown in Table 2.
(2) flavones content
Appropriate rutin standard items are accurately weighed, the rutin standard items stoste of ethyl alcohol configuration 0.2mg/ml is used.It takes respectively
0ml, 0.1ml, 0.2ml, 0.3ml, 0.4ml, 0.5ml rutin standard items stoste, the 5%Na2CO3 for sequentially adding 0.04ml are molten
Liquid shakes up, and 10% Al (NO3), 3 solution of 0.04ml is added after 5min, is settled to 1ml.It is surveyed after reacting 15min at room temperature
Determine the light absorption value at 510nm.Using concentration as abscissa, absorbance is that ordinate makees standard curve, obtains regression equation y=
1.3114x+0.0409 R2=0.9987.Sample is with 50% ethyl alcohol dissolved dilution to 1mg/mL.0.5ml distilled water is added,
0.05ml sample liquid sequentially adds the 5%Na2CO3 solution of 0.04ml, shakes up, and the 10% of 0.04ml is added after 5min
3 solution of Al (NO3), is settled to 1ml.The light absorption value at 510nm is measured after reaction 15min at room temperature.It is calculated according to standard curve
Flavones content is obtained, the results are shown in Table 2.
Table 2
To sum up, Semen Coicis water extract of the invention is rich in total Soluble Sugar, protein, amino acid, and Semen Coicis alcohol extract is rich in more
Phenol and flavones.The present invention is enriched the active material in Semen Coicis by step by step arithmetic, improves the utility value of Semen Coicis.
Claims (8)
1. a kind of method of step by step arithmetic Semen Coicis water extract and Semen Coicis alcohol extract from Semen Coicis, which is characterized in that including walking as follows
It is rapid:
(1) it dries after cleaning Semen Coicis, then crushes, cross 60-100 mesh, obtain coixlacrymajobi powder, coixlacrymajobi powder is wrapped up with gauze
Afterwards, n-hexane is added, then shakes degreasing 1-2h in a water bath, takes out gauze packet, obtains degreasing coixlacrymajobi powder, it is spare after dry；
(2) the gauze packet equipped with degreasing coixlacrymajobi powder after drying is taken, is added in distilled water, is extracted in 65~70 DEG C of water-bath,
Then take out gauze be responsible for a task until it is completed it is dry after it is stand-by, supernatant is collected after centrifugation in extracting solution, after supernatant removes solvent, then is freezed dry
It is dry, obtain Semen Coicis water extract；
(3) the gauze packet in step (2) is taken, 50% ethanol solution is added, is extracted in 65~70 DEG C of water-bath, then removes yarn
Extracting solution is collected after centrifugation supernatant, after supernatant removes solvent, then is freeze-dried, obtains Semen Coicis alcohol extract by cloth bag.
2. as described in claim 1 from Semen Coicis step by step arithmetic Semen Coicis water extract and Semen Coicis alcohol extract method, feature exists
In in step (1), the solid-liquid ratio of coixlacrymajobi powder and n-hexane is 1:4.
3. as described in claim 1 from Semen Coicis step by step arithmetic Semen Coicis water extract and Semen Coicis alcohol extract method, feature exists
In in step (1), bath temperature is 55~60 DEG C.
4. as described in claim 1 from Semen Coicis step by step arithmetic Semen Coicis water extract and Semen Coicis alcohol extract method, feature exists
In in step (2), the solid-liquid ratio of degreasing coixlacrymajobi powder and distilled water is 1:8 after drying.
5. as described in claim 1 from Semen Coicis step by step arithmetic Semen Coicis water extract and Semen Coicis alcohol extract method, feature exists
In in step (2), centrifugation number is centrifugal rotational speed 6000r/min more than twice, and every centrifugation time is 10min.
6. as described in claim 1 from Semen Coicis step by step arithmetic Semen Coicis water extract and Semen Coicis alcohol extract method, feature exists
In, in step (2) and (3), extraction time 4-5h.
7. as described in claim 1 from Semen Coicis step by step arithmetic Semen Coicis water extract and Semen Coicis alcohol extract method, feature exists
In, in step (3), solid-liquid ratio 1:8.
8. the method for step by step arithmetic Semen Coicis water extract and Semen Coicis alcohol extract as described in any one of claim 1 to 7 from Semen Coicis,
It is characterized in that, centrifugation number is centrifugal rotational speed 6000r/min more than twice in step (3), every centrifugation time is
10min。
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Cited By (2)
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| CN112629961A (en) * | 2019-09-24 | 2021-04-09 | 北京大学 | Coarse cereal gradual programmed extraction method and application thereof |
| CN114438160A (en) * | 2020-11-06 | 2022-05-06 | 北京大学 | Method for screening and optimizing coarse cereal compound composition and proportion and application thereof |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN112629961A (en) * | 2019-09-24 | 2021-04-09 | 北京大学 | Coarse cereal gradual programmed extraction method and application thereof |
| CN114438160A (en) * | 2020-11-06 | 2022-05-06 | 北京大学 | Method for screening and optimizing coarse cereal compound composition and proportion and application thereof |
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