CN109197874A - Bactericidal composition and its application containing pyraziflumid and fluazinam - Google Patents
Bactericidal composition and its application containing pyraziflumid and fluazinam Download PDFInfo
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- CN109197874A CN109197874A CN201811184014.8A CN201811184014A CN109197874A CN 109197874 A CN109197874 A CN 109197874A CN 201811184014 A CN201811184014 A CN 201811184014A CN 109197874 A CN109197874 A CN 109197874A
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- fluazinam
- pyraziflumid
- bactericidal composition
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/48—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with two nitrogen atoms as the only ring hetero atoms
- A01N43/60—1,4-Diazines; Hydrogenated 1,4-diazines
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
- A01N43/40—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom six-membered rings
Abstract
The present invention relates to a kind of bactericidal composition, the bactericidal composition contains pyraziflumid and fluazinam, and the weight ratio of the pyraziflumid and fluazinam is 10:1~1:10.Show that bactericidal composition of the invention can administer the drug resistance that Sclerotinia sclerotiorum generates current fungicide such as benzimidazole and dicarboximide fungicide by indoor biometrics and field control effectiveness test result, its bactericidal effect is significant, effective Instructing manufacture application is played in prevention and treatment Cruciferae, pulse family, Solanaceae, rutaceae disease, it reduces sclerotinia sclerotiorum to endanger caused by rape, improves the yield and quality of rape.
Description
[technical field]
Technical field of pesticide involved in the present invention, especially a kind of Bactericidal composite containing pyraziflumid and fluazinam
The application of object and the bactericidal composition in sclerotinia sclerotiorum.
[background technique]
Pyraziflumid is pyrazinamide class fungicide (the exploitation hair NNF- by Nihon Nihyaku Co., Ltd's exploitation
0721), it is succinate dehydrogenase inhibitors (SDHI).It is mainly used in rice, fruit tree, vegetables and lawn etc., to powdery mildew, black
Star disease, gray mold, sclerotiniose, ring spot, fruit blotch and coin pinta etc. have good control efficiency.
Fluazinam, english common name fluazinam are a kind of dinitroanilines developed by Japanese Shi Yuan company
Protective fungicide, it is mould to Alternaria, Botrytis, Phytophthora, single shaft to plurality of plant diseases activity with higher
Category, Sclerotinia and Venturia germ are highly effective.Fight the Sclerotina Sclerotiorum in Winter Rape of dicarboximide class and benzimidazole germicide
Core germ has good preventive effect, and milk rain drop erosion, lasting period are long.
In China, sclerotinia sclerotiorum is first of three major disease of rape, and pathogen sclerotinite can infect 75 sections 278 category
400 various plants.Sclerotinia sclerotiorum has generation in China major part rape producing region, wherein with the production of middle and lower reach of Yangtze River basin rape
Area is the most serious, and every annual morbidity is heavier, and production loss is up to 80%, and oil content is caused to fall sharply, and seriously affects the yield of rape
And quality.Currently, sclerotinia sclerotiorum is mainly controlled by chemical pesticides.But long-term single use dicarboximide class and benzene
And imidazoles fungicide causes prevention and treatment to be lost so that the sclerotinia sclerotiorum in part rape producing region develops drug resistance to these two types of medicaments
It loses.Reasonable chemical bactericide combination or mixture, which has, to be expanded fungicidal spectrum, improves control efficiency, delays drug resistance and drug resistance
Occur and the positive features such as development.Therefore, it is badly in need of developing the efficient, new type bactericide of wide spectrum, low toxicity, safety or compounding agent carrys out generation
For above-mentioned medicament for preventing and treating sclerotinia sclerotiorum.
[summary of the invention]
The object of the present invention is to provide a kind of economic, low toxicity, efficiently, low-residual, Environmental compatibility it is good be directed to Sclerotina Sclerotiorum in Winter Rape
The bactericidal composition of core disease.
To achieve the goals above, the present invention provides a kind of bactericidal composition, and the bactericidal composition contains
The weight ratio of pyraziflumid and fluazinam, the pyraziflumid and fluazinam is 10:1~1:10.
According to a kind of preferred embodiment, the weight ratio of the pyraziflumid and fluazinam is 3:1~1:3.
The present invention also provides a kind of agricultural bacteriocide, the fungicide contains 0.5~90.0% aforementioned to kill by weight
Bacteria composition and surplus are agriculturally acceptable carrier and auxiliary agent.
Preferably, the agricultural bacteriocide contains 10~80% aforementioned bactericidal composition by weight and surplus for agricultural
Upper acceptable carrier and auxiliary agent.
Optionally, the dosage form of the agricultural bacteriocide can be water dispersion granule, wettable powder, suspending agent, missible oil,
Aqueous emulsion or microemulsion.
The present invention also provides the application of above-mentioned bactericidal composition or agricultural bacteriocide in prevention and treatment sclerotinia sclerotiorum.
The present invention also provides above-mentioned bactericidal composition or agricultural bacteriocide in prevention and treatment Cruciferae, pulse family, Solanaceae, Rutaceae
Application in plant disease.
The present invention is described in more detail below.
The present invention relates to a kind of agriculture bactericidal compositions.The bactericidal composition contains pyraziflumid and fluazinam,
The weight ratio of the pyraziflumid and fluazinam is 10:1~1:10.
The concrete structure formula of Pyraziflumid are as follows:
The compound is the compound (3-21) prepared according to the record of PCT application WO2007072999.Preferably,
The weight ratio of pyraziflumid and fluazinam is 3:1~1:3.In bactericidal composition of the present invention, effective component
When the amount of pyraziflumid is in the range, if the amount of fluazinam be more than the amount range, may because with
Amount is high, safety decline, easily generation phytotoxicity.
Similarly, when the amount of effective component fluazinam is in the range, if the amount of pyraziflumid is more than described
The range of amount, then may be because of dosage height, safety decline, easily generation phytotoxicity.
The present invention relates to a kind of agricultural bacteriocides.The agricultural bacteriocide contains by weight described in 0.5~90.0%
Bactericidal composition and surplus be agriculturally acceptable carrier and auxiliary agent.
In the present invention, the carrier or auxiliary agent are it should be understood that for improving medicine in Pesticide formulation or use
Agent physicochemical property, improve drug effect, be readily transported storage etc. performances auxiliary substances, such as wetting agent, emulsifier, dispersing agent,
Sticker, stabilizer or synergist etc..According to the present invention, all that there are these performances and do not have to fungicide of the present invention any negative
The chemical substance that face is rung may be used to the present invention, they are all within protection scope of the present invention.
It according to the present invention, can be because of effective component if the bactericidal composition content of the fungicide is lower than 0.5%
It is low, it is unfavorable for the manufacturing of product from economic angle；If the bactericidal composition content of the agricultural bacteriocide is higher than
When 90.0%, then because of the too high performance for being unfavorable for drug effect of content and phytotoxicity can be also easy to produce；Therefore, the agricultural insecticidal of the fungicide
It is appropriate that composition levels, which are 0.5~90.0%,.
Preferably, the agricultural bacteriocide contains bactericidal composition described in 10~80% by weight and surplus is agriculture
Acceptable carrier and auxiliary agent in industry.
In the present invention, the carrier used is solid carrier or liquid-carrier.
The solid carrier is selected from silicate, carbonate, sulfate, oxide, alkali metal salt, vegetalitas carrier or conjunction
At earthfill rockfill concrete riprap RCC carrier etc..
Wherein silicate, carbonate, sulfate and oxide carrier are mineral substance carrier.
For example, silicate carrier is kaolin, sepiolite, nacrite, montmorillonite, mica, vermiculite, pyrophyllite or talcum
Deng.
Carbonate support is calcium carbonate or dolomite etc..
Sulfate carrier is ammonium sulfate, sodium sulphate or calcium sulfate etc..
Oxide carrier is quick lime, magnesium lime or diatomite etc..
Alkali metal salt carrier is potassium chloride or sodium chloride etc..
Vegetalitas carrier is citrus pulp, corncob cellulose, rice husk powder, rice husk, big soya bean stem meal, tobacco powder, walnut shell or sawmilling
Powder etc..
Synthetic filling class carrier is winnofil hydrate, winnofil or white carbon black etc..
These solid carriers that the present invention uses are sold known to technical staff, currently on the market in the art
The by-product of the product or widely known agricultural byproducts processing sold.
The liquid-carrier (solvent) is selected from water, aromatic hydrocarbons, chlorinated aliphatic hydrocarbon, aliphatic hydrocarbon, alcohols, ethers, ketone, plant
Oil, methylated vegetable oils or other special solvents.
For example, aromatic hydrocarbons carrier is benzene, dimethylbenzene, toluene, alkylbenzene, alkylnaphthalene and chlorinated aromatic hydrocarbons etc..
Chlorinated aliphatic hydrocarbon carrier is vinyl chloride, chloroform, methylene chloride, chloroform, carbon tetrachloride and more chloroethanes etc..
Aliphatic hydrocarbon carrier is petroleum distillate, hexamethylene, light mineral oil or paraffin etc., such as limited by Jiangsu China human relations chemical industry
The S-200 fat hydrocarbon product of company's sale being mainly made of octane and heptane.
Alcoholic vehicle is methanol, ethyl alcohol, isopropanol, butanol, isobutanol, ethylene glycol, propylene glycol, glycerine or fatty alcohol
Deng.
Ethers carrier is Propylene Glycol ether, ethohexadiol ether or petroleum ether etc..
Ketone is acetone, methyl ethyl ketone, methyl iso-butyl ketone (MIBK), cyclohexanone, isophorone or N- methyl-pyrrolidon etc..
In the present invention, the special solvent is it should be understood that the solvent being used in mixed way with one or more of solvents.It is described
Solvent in special solvent is dimethylformamide, dimethyl sulfoxide, polyethylene glycol or nitrile etc..
Vegetable oil is soybean oil, rapeseed oil, peanut oil or sesame oil etc..
Methylated vegetable oils are methylated soya oil, rapeseed oil, peanut oil or sesame oil etc..The methylation that the present invention uses
Vegetable oil is all in current market sales of product.
The liquid-carrier that the present invention uses all is in the art known to technical staff, current market sales of
Product, for example, sold by Sinopec Group Hangzhou Refinery light mineral oil, by Shanghai Sheng Jun Industry investment Co., Ltd
The Propylene Glycol ether of sale, ethohexadiol ether, methyl iso-butyl ketone (MIBK), isophorone, dimethylformamide.
When using a kind of above-mentioned organic solvent, this organic solvent can with single use, can also from it is other different
Organic solvent is used in mixed way, or is used in mixed way with water.When used as a mixture, mixed proportion can according to actual use situation into
Row selection, this is that there is no hell and high waters for those skilled in the art.
The auxiliary agent that the present invention uses selected from emulsifier, dispersing agent, stabilizer or wetting agent surfactant, disappear
Infusion, thickener, suspending agent and antifreezing agent.
The surfactant is the surfactant used in emulsifier, dispersing agent, stabilizer or wetting agent.
The surfactant that the present invention uses can be ionic or non-ionic, such as polyacrylic acid sodium salt and calcium
Salt, lignin sulfonic acid sodium salt and calcium salt；With at least 12 carbon atom fatty acids or fatty amine and ethylene oxide and/or epoxy third
The polycondensation product of alkane；Glycerol, dodecanol -1, tetradecanol -1, sorbierite, sucrose or pentaerythritol fatty ester and they
With the polycondensation product of ethylene oxide and/or propylene oxide；The sulfate or sulfonate of the polycondensation product；With at least ten carbon
Atom alkyl sulfuric acid or sulfonate alkali metal or alkali salt, preferably sodium salt, as lauryl sodium sulfate, secondary alkyl sulfuric acid
Sodium, sulfonated castor oil sodium salt, alkylaryl sodium sulfonate or neopelex；Ethylene oxide polymer or ethylene oxide and ring
Ethylene Oxide copolymer.
Emulsifier that the present invention uses is allowed to shape it should be understood that one kind can improve each phase interfacial surface tension in emulsion
At the substance of uniform and stable dispersion or emulsion.It is all that there are these performances and agricultural bacteriocide of the present invention is not appointed
The chemical substance of what negative effect may be used to the present invention, they are all within protection scope of the present invention.
The emulsifier that the present invention uses is nonionic emulsifier.For example, the nonionic emulsifier is selected from polyoxy
Ethylene fatty acid ester, polyoxyethylene aliphatic alcohol ether and Polyoxyethylene fatty ammonia, as ethoxylated castor oil, polyoxyethylene (n20) benzene
Ethyl phenolic group oleic acid ester, alkylaryl polyoxyethylene poly-oxygen propylene aether, alkylaryl formaldehyde resin polyoxyethylene ether, triphen second
Alkenyl phenol polyethenoxy (n20), ether phosphoric acid triethanolamine salt, Diphenethyl phenolic group polyoxyethylene ether (n18) formaldehyde condensation products,
Diphenyl phenol polyethenoxy (n18) polyethenoxy ether (n20) etc., these nonionic emulsifier are all current market sales of
Product, such as the polyoxyethylene (n provided by Jiangsu Kai Yuan Science and Technology Ltd.20) phenethyl phenolic group oleic acid ester, alkylaryl
Formaldehyde resin polyoxyethylene ether, sale diphenyl phenol polyethenoxy (n18) polyethenoxy ether (n20).The emulsification that the present invention uses
Agent can be commercial emulsifier, such as agriculture breast 0201B, agriculture breast 0203B, agriculture breast 100#, agriculture breast 500#, agriculture breast 600#, agriculture breast 600-
2#, agriculture breast 1601, agriculture breast 2201, agriculture breast NP-10, agriculture breast NP-15, agriculture breast 507#, agriculture breast OX-635, agriculture breast OX-622, agriculture breast
OX-653, agriculture breast OX-667, agriculture breast CS-7 or peaceful cream 36#。
Dispersing agent that the present invention uses is it should be understood that a kind of in the molecule while having lipophilic fraction and hydrophily portion
Point interfacial agent, it be it is a kind of will can be difficult to be dissolved in the solid in liquid evenly dispersed with liquid particles, simultaneously and also
It can be prevented to settle and agglomerate, form the substance of stable suspension.It is all to there are these performances and to agricultural bacteriocide of the present invention
There is no the chemical substance of any negative effect to may be used to the present invention, they are all within protection scope of the present invention.
The dispersing agent that the present invention uses is, for example, sodium lignin sulfonate, pull open powder, calcium lignosulfonate, methyl naphthalene sulfonic acid first
Aldehyde condensate, naphthalene sulfonic acid-formaldehyde condensation product, methanonaphthalene sodium sulfonate, oleic acid methylaminoethyl sodium sulfonate, epoxidized polyether, to uncle
Butyl ether, dibutyl naphthalenesulfonic acid formaldehyde condensation produc, phosphate such as calgon, alkylphenol-polyethenoxy based phosphates, alkyl
Phenol polyethenoxy base ether formaldehyde condensation products sulfate, the contracting of N- methyl-fatty acyl group-sodium taurocholate alkyl phenol polyoxyethylene ether formaldehyde
Close object, aralkyl phenol polyethenoxy ether phosphate alkyl phenol polyoxyethylene ether, castor oil ethylene oxide adduct, ethylene oxide-
Propylene oxide block copolymer APES formaldehyde condensation product, alkylphenol-polyethenoxy polyethenoxy ether and methyl are fine
Dimension element.
The dispersing agent that the present invention uses all is current market sales of product, such as by Nanjing Teva-Chem. Co., Ltd.
N- methyl-fatty acyl group-sodium taurocholate the APES formaldehyde condensation product of sale, castor oil ethyleneoxide addition
Object, ethylene oxide-propylene oxide block copolymer APES formaldehyde condensation product, alkylphenol-polyethenoxy Ji Mijia
Aldehyde condensate sulfate.
The stabilizer that the present invention uses is, for example, sodium lignin sulfonate.The stabilizer that the present invention uses all is currently on the market
The product of sale, such as the lignin series of products sold by virtue Visco Co..
Wetting agent that the present invention uses it should be understood that one kind can reduce solid material surface tension or interfacial tension,
Water easily spread out on the surface thereof and by solid material soak substance.It is all to there are these performances and to agricultural sterilization of the invention
Agent does not have the chemical substance of any negative effect to may be used to the present invention, they are all within protection scope of the present invention.
The wetting agent that the present invention uses is selected from sldium lauryl sulfate, alkylol polyoxyethylene base ether sodium sulfate, octylphenol polyethylene
Oxyethylene group ether sulfate, alkylphenol polyoxyethylene ether formaldehyde condensate sulfate, sodium alkyl sulfonate, dodecyl benzene sulfonic acid
Sodium, Negel, dialkyl succinylsuccinate sulfonate, lauryl alcohol polyethylene glycol oxide base ether sulfonic acid sodium, alkyl amide sodium taurocholate, rouge
Fat alcohol polyoxyethylene ether, alkylphenol-polyethenoxy base ether, polyoxyethylene polyoxypropylene block polymer, sorbitan fatty
Acid esters polyoxyethylene ether etc..
The wetting agent that the present invention uses all is current market sales of product, such as is sold by Xing Fei Chemical Co., Ltd.
Alkylol polyoxyethylene base ether sodium sulfate, alkylphenol polyoxyethylene ether formaldehyde condensate sulfate, lauryl alcohol polyethylene glycol oxide
Base ether sulfonic acid sodium, sorbitan fatty acid ester polyoxyethylene ether.
In addition to above-mentioned surfactant, there are also defoaming agent, thickener, suspending agent and antifreezing agents for the auxiliary agent that the present invention uses.
The defoaming agent that the present invention uses is it should be understood that a kind of substance with chemistry and surface chemistry defoaming effect.It is all
There is no the chemical substance of any negative effect to may be used to the present invention with these performances and to agricultural bacteriocide of the present invention, it
All within protection scope of the present invention.
The defoaming agent is selected from GPE, silicone, C8~10Fatty alcohol, C10~20Saturated fatty acid or amide defoaming agent；
The defoaming agent that the present invention uses all is current market sales of product, such as is sold by Nanjing Jie Run Co., Ltd
GPE, C10~20Saturated fatty acid, amide defoaming agent.
Thickener that the present invention uses be can be improved and be killed it should be understood that one kind can be such that agricultural bacteriocide of the present invention emulsifies
Microbial inoculum viscosity simultaneously reaches stable substance.It is all that there are these performances and do not have any negative shadow to agricultural bacteriocide of the present invention
Loud chemical substance may be used to the present invention, they are all within protection scope of the present invention.
The thickener is selected from carboxymethyl alcohol, polyvinyl alcohol, polyvinyl acetate, xanthan gum, gelatin, Arabic tree
Glue, polyvinylpyrrolidone, aluminum magnesium silicate, polyvinyl alcohol, polyethylene glycol, phenolic resin, shellac, carboxymethyl cellulose or seaweed
Sour sodium；
The thickener that the present invention uses all is current market sales of product, such as by Beijing Green Tai Mu Co., Ltd
Carboxymethyl alcohol, the polyvinylpyrrolidone of sale.
The suspending agent that the present invention uses is it should be understood that one kind can increase agricultural bacteriocide viscosity of the present invention to reduce
Wherein particle sinking speed or the increase hydrophilic substance of particle.It is all that there are these performances and do not have to agricultural bacteriocide of the present invention
There is the chemical substance of any negative effect to may be used to the present invention, they are all within protection scope of the present invention.
The suspending agent is selected from glycerol, Arabic gum, tragacanth, peach gum, methylcellulose, sodium carboxymethylcellulose
Or hydroxypropyl cellulose.
The suspending agent that the present invention uses all is current market sales of product, such as by Nanjing Guan Hua trade Co., Ltd
Xanthan gum, peach gum, the sodium carboxymethylcellulose of sale.
The antifreezing agent that the present invention uses is it should be understood that one kind can prevent agricultural bacteriocide of the present invention in microthermal climate ring
The substance freezed under border.It is all that there are these performances and do not have the chemistry of any negative effect to agricultural bacteriocide of the present invention
Substance may be used to the present invention, they are all within protection scope of the present invention.
The antifreezing agent is selected from glycerol, urea, ethylene glycol or propylene glycol.
The antifreezing agent that the present invention uses all is current market sales of product.
According to the present invention, the agricultural bacteriocide is water dispersion granule, wettable powder, suspending agent, missible oil, water and milk
The dosage forms such as agent or microemulsion.
Fungicide of the present invention can be applied using conventional method, such as spray, is spraying.Its amount of application can be according to weather item
Part or crop growthing state and change, can disease occur before or after apply.The duration of protective effect usually with
The content of single compound is related in composition, also related to extraneous factor, such as weather, but can by using dosage form appropriate
To slow down the influence of weather.
The present invention reuses resistance problems caused by single fungicide for field for a long time, with new type bactericide
Succinate dehydrogenase class (SDHI) class fungicide and dinitroaniline fungicide fluazinam carry out binary built, pass through indoor poison
Power measurement, synergistic effect Mechanism Study and field efficacy experiment, confirm answering in pyraziflumid and fluazinam 3:1~1:3
There is apparent synergistic effect in vitro with fungicide, wherein when pyraziflumid and fluazinam proportion are 2:1
Synergistic effect is maximum, and this proportion also shows significant synergistic effect in field control effectiveness test.With single bactericide phase
Than with below the utility model has the advantages that
After two kinds of active components are by different proportion compounding in composition, have under isolated condition and field trial significant
Synergistic effect and lasting effect.
The invention belongs to the formulas of binary built fungicide, and field dosage and times for spraying can be greatly reduced, drop
Low production cost, reduces environmental pollution and pesticide residue.
The present invention is made of the effective component of different role mechanism, increases the action site to Sclerotinia sclerotiorum, has
Conducive to the generation for overcoming and delaying sclerotinia sclerotiorum drug resistance.
The present invention can administer Sclerotinia sclerotiorum and sterilize to current fungicide such as benzimidazole and dicarboximide class
The drug resistance that agent generates.
The formula bactericidal effect of fungicide is significant in the present invention, and the effective Instructing manufacture application of energy reduces sclerotinia sclerotiorum
It is endangered caused by rape, improves the yield and quality of rape.
[Detailed description of the invention]
Fig. 1 is the variation that different time different agents handle mycelial growth inhibition rate.
[specific embodiment]
One, biological activity determination
In order to further appreciate that the virulence of fluazinam and pyraziflumid, a large amount of recipe determination experiments have been carried out.
1, to the indoor toxicity test of Sclerotinia sclerotiorum
The recipe determination of compounding agent is carried out as research object using sclerotinia sclerotiorum.
According to mycelial growth rate method, sclerotinia sclerotiorum bacteria strain is connect and cultivates 2d on PDA plate, then beaten at edge
Fresh bacterium dish (diameter: 5mm) is taken to be connect respectively in the PDA plate for containing a series of concentration fluazinams and pyraziflumid medicament
On, while also access contains fluazinam (referred to as " F ", similarly hereinafter) and pyraziflumid (referred to as " P ", similarly hereinafter) with a series of differences
The PDA of concentration proportioning is containing in medicine plate.Medicine plate will be contained to be placed on 25 DEG C of incubator culture 2d and then survey its colony diameter, each processing is set
Three repetitions are set, calculate growth inhibition ratio using the PDA plate of not adding medicine as control.Each medicine is calculated using probit analysis
Agent calculates coefficient of synergism to concentration in effective inhibition of target pathogens, and experiment is in triplicate.
According to Wadley method/standard, coefficient of synergism SR>1.5 show that composition shows as synergy, coefficient of synergism 0.5<
SR < 1.5 show that composition shows as being added, and coefficient of synergism SR < 0.5 shows that composition shows as antagonism.
1 pyraziflumid of table and fluazinam different agents are with the virulence for comparing Sclerotinia sclerotiorum
Table 1 statistics indicate that on PDA plate with various concentration match pyraziflumid and fluazinam dosages distribution
To the EC of Sclerotinia sclerotiorum strain50It is discrepant, wherein EC50Numerical value is between 0.0062~0.0094 μ g/mL.Wherein
Pyraziflumid and fluazinam proportion have prominent synergistic effect when being 2:1, coefficient of synergism 1.5669 illustrates under this proportion
Pyraziflumid and fluazinam collective effect have apparent synergistic effect.
2, influence when Pyraziflumid and fluazinam proportion are 2:1 to Sclerotinia sclerotiorum silk growth rate
In order to measure influence when pyraziflumid and fluazinam proportion are 2:1 to mycelial growth rate, by rape
Hyphal cluster germ bacterial strain connects and cultivates 2d on PDA plate, then beats at edge and takes fresh bacterium dish (diameter: 5mm) to connect respectively to contain
0.05 μ g/mLpyraziflumid, fluazinam and pyraziflumid and fluazinam proportion are on the PDA plate of 2:1, with not
Three repetitions are arranged as blank control, every processing in the PDA of adding medicine, and plate is cultivated under the conditions of being placed in 25 DEG C.Respectively 12h,
For 24 hours, 36h, 48h measure colony diameter, calculate the inhibition hundred that medicament grows mycelia with not comparing containing any medicament
Divide rate, test is repeated 3 times.
As Fig. 1's, the experimental results showed that, over time, pyraziflumid grows mycelia before 36h
Inhibiting rate reduce, inhibiting rate is gradually increased fluazinam over time, tend to be steady after 36h, and pyraziflumid and
Mycelial growth inhibition rate is basically unchanged when fluazinam is compounded with 2:1, but is consistently higher than pyraziflumid and fluazinam and is individually made
The growth inhibition ratio of used time.Show that the composition of pyraziflumid and fluazinam has the higher growth to Sclerotinia sclerotiorum
Inhibiting rate.
Two, the preparation of agricultural bacteriocide of the present invention
Example 1:pyraziflumid fluazinam suspending agent (pyraziflumid fluazinam=2:1)
It is prepared according to the common process of suspending agent, wherein pyraziflumid 10g, fluazinam 5g, sodium lignin sulfonate
(water dispersant) 4g, neopelex (wetting agent) 2g, ethylene glycol (antifreeze) 4g, bentonite (thickener) 0.15g,
Organic silicone (defoaming agent) 0.4g, deionized water complement to 100g.
Three, field efficacy of the compounding agent in sclerotinia sclerotiorum when Pyraziflumid and fluazinam proportion are 2:1
Field information example 1
Pyraziflumid and fluazinam are measured with 2:1 compounding agent to the field control effect of rape sclerotium, chooses 2016-
Incidence is investigated in the experimental plot on Baima lake farm of the heavier Jiangsu Province's Taizhou City of intercurrent disease in 2017, carries out field efficacy
Test.Experimental field is divided into 21 cells, each plot area is 20m2, treatment agent effective ingredient consumption is
Pyraziflumid10g/ mus, 20g/ mus；10g/ mus of fluazinam, 20g/ mus；What pyraziflumid and fluazinam were compounded with 2:1
Dosage is 10g/ mu, 20g/ mu, and set clear water and compare, each processing 3 repetitions of setting, all processing cell random distributions.
Start spray when growth of rape reaches 90% to stem flowering rate and (uses Singapore's benefit agriculture Jacto 16LITROS type sprayer
Spraying, aperture < 7mm, medicament does not stay raffinate).In rape harvest the last week, incidence is investigated, records diseased plant number, sick grade.
Sclerotinia sclerotiorum grade scale:
0 grade: disease-free；
1 grade: one big branch morbidity or stem 3cm or so morbidity；
2 grades: two big branch morbidity or 4~7cm of stem morbidity；
3 grades: three big branch morbidity or 7~10cm of stem morbidity；
4 grades: four or more big branch morbidity or stem 10cm or more fall ill.
Field control effect of the compounding agent to sclerotinia sclerotiorum when 2 pyraziflumid of table and fluazinam proportion are 2:1
Field control effectiveness test proves that pyraziflumid and two kinds of fungicide of fluazinam have compatibility, and the two compounding makes
Its bactericidal effect is significant, shows as significant cooperative synergism effect and improves field efficacy, therefore can effective Instructing manufacture
Using reduction sclerotinia sclerotiorum is endangered caused by rape, improves the yield and quality of rape.Pyraziflumid and fluazinam
Compounding 3:1~1:3 show as collaboration increase, wherein optimum compound proportion 2:1, in terms of inhibiting mycelial growth rate,
Inhibiting effect significantly improves, and there are significant differences compared with single dose.Field control effectiveness test shows pyraziflumid and fluorine pyridine
Compounding agent is aobvious to sclerotinia sclerotiorum and Cruciferae, pulse family, Solanaceae, the preventive effect of rutaceae disease when amine proportion is 2:1
Write the preventive effect being higher than when each medicament is used alone.
In summary, a large amount of indoor biometrics and field control effectiveness test result show Bactericidal composite produce of the present invention
Raw has the beneficial effect that
Compared with single dose, which has significant synergistic effect to disease, improves bactericidal effect；
Times for spraying is reduced, drug cost is reduced, reduces pollution and pesticide residue to environment；
Composition is conducive to overcome and delay the drug resistance of germ since different mechanism of action, action site increase
It generates.The present composition is without obvious harmful effects to trial crops, and leaf color, growing way etc. are all normal, and safety is good, meets agriculture
The security requirement of medicine preparation.
Claims (8)
1. a kind of bactericidal composition, the bactericidal composition contains pyraziflumid and fluazinam, the pyraziflumid
Weight ratio with fluazinam is 10:1~1:10.
2. bactericidal composition according to claim 1, it is characterised in that the weight of the pyraziflumid and fluazinam
Than for 3:1~1:3.
3. bactericidal composition according to claim 1, it is characterised in that the weight of the pyraziflumid and fluazinam
Than for 2:1.
4. a kind of agricultural bacteriocide, it is characterised in that it contains 0.5~90.0% of any of claims 1 or 2 kills by weight
Bacteria composition and surplus are agriculturally acceptable carrier and auxiliary agent.
5. agricultural bacteriocide according to claim 4, it is characterised in that it contains 10~80% claim by weight
Any bactericidal composition and surplus are agriculturally acceptable carrier and auxiliary agent in 1-3.
6. agricultural bacteriocide according to claim 4, it is characterised in that its dosage form is water dispersion granule, wettable powder
Agent, suspending agent, missible oil, aqueous emulsion or microemulsion.
7. any bactericidal composition or according to any one of claim 3-6 claim institute in -3 according to claim 1
State application of the agricultural bacteriocide in prevention and treatment sclerotinia sclerotiorum.
8. bactericidal composition described in any one of -3 or according to any one of claim 3-7 claim according to claim 1
Application of the agricultural bacteriocide in prevention and treatment Cruciferae, pulse family, Solanaceae, rutaceae disease.
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| CN201811184014.8A CN109197874A (en) | 2018-10-11 | 2018-10-11 | Bactericidal composition and its application containing pyraziflumid and fluazinam |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201811184014.8A CN109197874A (en) | 2018-10-11 | 2018-10-11 | Bactericidal composition and its application containing pyraziflumid and fluazinam |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101341136A (en) * | 2005-12-22 | 2009-01-07 | 日本农药株式会社 | Pyrazinecarboxamide derivatives and plant disease controlling agents containing the same |
| WO2016060230A1 (en) * | 2014-10-16 | 2016-04-21 | 日本農薬株式会社 | Horticultural fungicide and method of using same |
| CN108184861A (en) * | 2016-12-08 | 2018-06-22 | 深圳诺普信农化股份有限公司 | A kind of bactericidal composition and its application containing pyraziflumid |
| CN108200911A (en) * | 2017-07-25 | 2018-06-26 | 吉林农业科技学院 | A kind of composition comprising biphenyl pyrazine bacterium amine and application thereof |
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2018
- 2018-10-11 CN CN201811184014.8A patent/CN109197874A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101341136A (en) * | 2005-12-22 | 2009-01-07 | 日本农药株式会社 | Pyrazinecarboxamide derivatives and plant disease controlling agents containing the same |
| WO2016060230A1 (en) * | 2014-10-16 | 2016-04-21 | 日本農薬株式会社 | Horticultural fungicide and method of using same |
| CN108184861A (en) * | 2016-12-08 | 2018-06-22 | 深圳诺普信农化股份有限公司 | A kind of bactericidal composition and its application containing pyraziflumid |
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| 张宝俊等: "新型杀菌剂Pyraziflumid的合成及生物活性", 《世界农药》 * |
| 杨吉春等: "近五年公开的新杀菌剂及其合成研究进展", 《精细化工中间体》 * |
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