CN109197871A - A kind of induction increases the inducer of red kiwi fruit amino acid content - Google Patents
A kind of induction increases the inducer of red kiwi fruit amino acid content Download PDFInfo
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- CN109197871A CN109197871A CN201811062138.9A CN201811062138A CN109197871A CN 109197871 A CN109197871 A CN 109197871A CN 201811062138 A CN201811062138 A CN 201811062138A CN 109197871 A CN109197871 A CN 109197871A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/44—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
- A01N43/36—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/48—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with two nitrogen atoms as the only ring hetero atoms
- A01N43/50—1,3-Diazoles; Hydrogenated 1,3-diazoles
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/40—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having a double or triple bond to nitrogen, e.g. cyanates, cyanamides
- A01N47/42—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having a double or triple bond to nitrogen, e.g. cyanates, cyanamides containing —N=CX2 groups, e.g. isothiourea
- A01N47/44—Guanidine; Derivatives thereof
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- Life Sciences & Earth Sciences (AREA)
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- Pest Control & Pesticides (AREA)
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- Dentistry (AREA)
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- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention discloses the inducers that a kind of induction increases red kiwi fruit amino acid content, it is calculated by mass percentage, it contains chitosan 0.5%~2.0%, amino acid 0.5%~1.5%, alginic acid 0.2%~1.0%, tetramycin 0.05%~0.15%, matrine 0.05%~0.15%, surplus are water.After inducer leaching fruit processing, the total amino acid content 20~30% of ' Red Male ' kiwifruit fruit is increased extremely significantly, improves the nutritional quality of fruit.Has many advantages, such as environmental-friendly, low in cost, significant effect and easy to spread.
Description
Technical field
The present invention relates to technique for culturing yangtao fields in agricultural, and in particular to a kind of induction increase red kiwi fruit ammonia
The inducer of base acid content.
Background technique
In recent years, one of the fine fruit tree species that Kiwi berry is paid much attention to as Guizhou Provincial Party committee, provincial government, the whole province macaque
Peach industry, which is presented, grows rapidly the impetus, has developed into Guizhou Province's characteristic fine fruit and local pillar industry.Main distribution
In the industry centralizations such as Guiyang, Lupanshui City, Qiandongnan Prefecture of Guizhou Province, Zunyi development zone.Red Male " Kiwi berry (accounts for the whole province 43.2%)
It is the excellent variety in Chinese gooseberry, in Guizhou Province, Lupanshui City has obtained greatly developing existing cultivated area nearly 100,000
Mu.It shakes off poverty as power-assisted the important industry of getting rich assaulted fortified position, ' Red Male ' Kiwi berry expands year by year in cultivated area inside and outside the province in recent years
, also paid much attention to by Guizhou provincial government of Provincial Party committee and its emphasis be classified as the poverty alleviation industry of " fission formula " development.Amino acid is
The important factor of the important component of kiwifruit fruit quality, and influence fruit fresh food, storage and processing etc..Currently, needle
Improvement method to kiwifruit fruit amino acid is mainly applying organic manure, oil cake etc., but these processing be higher cost and
It is ineffective.Based on this, the present invention provides the inducers that a kind of induction increases red kiwi fruit amino acid content, can have
Effect improves red kiwi fruit fruit total amino acid content, low in cost and significant effect.
Summary of the invention
It is existing to solve the object of the present invention is to provide the inducer that a kind of induction increases red kiwi fruit amino acid content
There are the problems such as technical costs is higher and ineffective.Has many advantages, such as easy to operate, low in cost, significant effect.
What the present invention was achieved through the following technical solutions:
The invention proposes the inducers that a kind of induction increases red kiwi fruit amino acid content, by mass percentage
It calculates, it contains chitosan 0.5%~2.0%, amino acid 0.5%~1.5%, alginic acid 0.2%~1.0%, tetramycin
0.05%~0.15%, matrine 0.05%~0.15%, surplus is water.It is by the component of following mass percent: shell is poly-
Sugared 1.0%, amino acid 0.7%, alginic acid 0.5%, tetramycin 0.1%, matrine 0.1%, surplus is water.
Wherein, in technical solution of the present invention chitosan be water solubility, deacetylation be greater than 95%, molecular weight be 10~
50KDa；The amino acid is proline, glycine, glutamic acid, phenylalanine, leucine, isoleucine, lysine, Soviet Union's ammonia
Acid, methionine, tryptophan, histidine, tyrosine, valine, alanine, serine, arginine, asparatate, half Guang ammonia
Any five kinds or more of combination in acid；Alginic acid is commercially available；Tetramycin is 0.3% tetramycin aqua；Matrine is
0.3% matrine water.
The application method of the inducer is that fruit processing is soaked after Kiwi berry is thanked and spent.
Compared with prior art, the present invention inducer of the present invention uses natural materials chitosan, amino acid, alginic acid, four
Mycin and matrine composition, can effectively improve kiwifruit fruit total amino acid content, low in cost and significant effect.
Specific embodiment
Below in conjunction with preferable embodiment, the present invention will be described.Specific material proportion described in embodiment,
Application method and its result are merely to illustrate the present invention, should not will not limit sheet described in detail in claims
Invention.
Embodiment:
(1) raw material sources: chitosan, Henan Hua Rui bioengineering Co., Ltd；Amino acid is self-control；Alginic acid, Henan
Chinese businessman's food additives Co., Ltd；Tetramycin, Liaoning Wei Ke bioengineering limited liability company；Matrine, Hebei Ward are rich
Pharmaceutcal corporation, Ltd.
(2) implementation column inducer composition and preparation
Inducer formula composition: chitosan 1.0%, amino acid 0.7%, alginic acid 0.5%, tetramycin 0.1%, kuh-seng
Alkali 0.1%, surplus are water.Preparation: the chitosan of required quality, amino acid, alginic acid, tetramycin and matrine are weighed mixed
It closes, the water of volume needed for being added, which sufficiently dissolves, both obtains inducer.
(3) test method
Experimental design: test, which is set, does not soak fruit 2 processing of fruit and leaching, and leaching fruit is handled using inducer.It does not soak at fruit and leaching fruit
Reason sets 4 repetitions respectively, and every repetition 2 trees, the leaching fruit time is April 14 (thank and take time), totally 8 cells, each cell and its
Surrounding sets protection row.
Experimental field it is located at the Guizhou Xiuwen County township the Gu Bao village Ping Tan Tai Feng (26 ° of 41 ' N, 106 ° of 22 ' E) kiwi fruit tree garden, it is average
Height above sea level is 1324m, and subtropical zone monsoon moist climate, average temperature of the whole year is 16 DEG C, and annual rainfall reaches 1293mm, the hydro-thermal same period.
It is yellow earth for examination orchard soil type, soil background pH value is 6.02, total nitrogen content 1.47g/kg, and the content of organic matter is
29.37g/kg, alkaline hydrolysis nitrogen content are 96.58mg/kg, and available phosphorus content 4.6mg/kg, effective potassium content is 0.58mg/kg,
Available potassium 29mg/kg, effective Zn content are 1.73mg/kg, effective iron content 51.31mg/kg, and effective manganese content is
19.13mg/kg, full Zn content are 41.66mg/kg, and full lead content is 6.3mg/kg, and full cadmium content is 0.46mg/kg, full selenium
Content is 0.188mg/kg.
Fruit Contents of Amino Acids method: measuring fruit hydrolysis amino acid when kiwifruit fruit reaches edible state,
It is measured using HPLC method.Instrument is ThermoFisher U3000 liquid chromatograph (the silent winged generation that of match, the U.S.).Sample treatment
It is as follows with analysis method:
1. sample treatment takes the sample of certain mass in the hydrolysis pipe of 20mL, hydrochloric acid (6 molL of 16mL are added-1)
Solution, vacuum outgas 30 minutes and nitrogen charging tube sealing；Under the conditions of 110 DEG C hydrolysis 22~cool down afterwards for 24 hours, afterwards with deionized water without
Damage is transferred in 50mL volumetric flask and constant volume.Accurately taking 1mL hydrolyzate, depickling is drained in a vacuum in bottle, and repetition drains 3
It is secondary spare.It is accurate that 1mL 0.02molL is added-1Hydrochloric acid solution sufficiently dissolves.Precision measures the 500 above-mentioned solution of μ L and is placed in plastics
In centrifuge tube, 1molL is added in precision-1The triethylamine acetonitrile solution of 250 μ L mixes, then accurate addition 0.1molL-125μL
Phenyl isothiocyanate acetonitrile solution mixes, and after being placed at room temperature for 1 hour plus 2mL n-hexane, violent shaking stand 10min, take lower layer
Upper machine analysis after 0.22 μm of water phase film filtering of solution.
2. sample analysis chromatographic condition mobile phase A: 0.1molL-1Sodium acetate solution-acetonitrile (93:7), Mobile phase B: second
Nitrile-water (8:2), chromatographic column: filler is octadecylsilane chemically bonded silica (4.6 × 250mm, 5 μm).Flow velocity is per minute
1.0mL, 10 μ L of sample volume, wavelength 254nm, column temperature is 40 DEG C.
(4) embodiment effect:
Table 1 shows that ' Red Male ' Kiwi berry essential amino acid rich in, inducer soak the amino acid of fruits
Total content extremely significant (P < 0.01), which is higher than, does not soak fruits.Wherein soak proline, glutamic acid, cystine, the silk ammonia of fruits
Acid, glycine, arginine, threonine, alanine, histidine, tyrosine, valine and extremely significant (the P < of leucine content
0.01) it is higher than and does not soak fruits.To sum up show that soaking fruit significantly increases the amino acid content of ' Red Male ' kiwifruit fruit, improves
The nutritional quality of fruit.
Table 1 does not soak fruit and leaching fruit processing ' Red Male ' kiwifruit fruit amino acid content compares
It is above specific application example of the invention, there are other embodiments of the invention, all to use equivalent replacement
Or the technical solution that equivalent transformation is formed, it all falls within protection scope of the presently claimed invention.
Claims (4)
1. the inducer that a kind of induction increases red kiwi fruit amino acid content, it is characterised in that: it is calculated by mass percentage,
It contain chitosan 0.5%~2.0%, amino acid 0.5%~1.5%, alginic acid 0.2%~1.0%, tetramycin 0.05%~
0.15%, matrine 0.05%~0.15%, surplus is water.
2. the inducer that induction according to claim 1 increases red kiwi fruit amino acid content, it is characterised in that: press matter
It measures percentage composition to calculate, it contains chitosan 1.0%, amino acid 0.7%, alginic acid 0.5%, tetramycin 0.1%, matrine
0.1%, surplus is water.
3. the inducer that induction according to claim 1 increases red kiwi fruit amino acid content, it is characterised in that: described
Chitosan is water solubility, and deacetylation is greater than 95%, and molecular weight is 10~50KDa；The amino acid be proline, glycine,
Glutamic acid, phenylalanine, leucine, isoleucine, lysine, threonine, methionine, tryptophan, histidine, tyrosine, figured silk fabrics
Propylhomoserin, alanine, serine, arginine, asparatate, any five kinds or more of combination in cysteine；Described four
Mycin is the tetramycin aqua of concentration 0.3%；The matrine is the matrine water of concentration 0.3%.
4. the inducer that induction according to claim 1 increases red kiwi fruit amino acid content, it is characterised in that: this is lured
The application method for leading agent is that fruit processing is soaked after Kiwi berry is thanked and spent.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201811062138.9A CN109197871A (en) | 2018-09-12 | 2018-09-12 | A kind of induction increases the inducer of red kiwi fruit amino acid content |
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| CN201811062138.9A CN109197871A (en) | 2018-09-12 | 2018-09-12 | A kind of induction increases the inducer of red kiwi fruit amino acid content |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107573179A (en) * | 2017-10-24 | 2018-01-12 | 贵州大学 | A kind of expensive long Kiwi berry Pesticidal foliage fertilizer and preparation method thereof |
| CN107646869A (en) * | 2017-09-15 | 2018-02-02 | 贵州大学 | A kind of derivant for promoting the sprouting of macaque peach seeds and growth of seedling |
| CN107793217A (en) * | 2017-10-24 | 2018-03-13 | 贵州大学 | A kind of red kiwi fruit Pesticidal foliage fertilizer and preparation method thereof |
-
2018
- 2018-09-12 CN CN201811062138.9A patent/CN109197871A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107646869A (en) * | 2017-09-15 | 2018-02-02 | 贵州大学 | A kind of derivant for promoting the sprouting of macaque peach seeds and growth of seedling |
| CN107573179A (en) * | 2017-10-24 | 2018-01-12 | 贵州大学 | A kind of expensive long Kiwi berry Pesticidal foliage fertilizer and preparation method thereof |
| CN107793217A (en) * | 2017-10-24 | 2018-03-13 | 贵州大学 | A kind of red kiwi fruit Pesticidal foliage fertilizer and preparation method thereof |
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Application publication date: 20190115 |