CN1091904A - Process for extracting edible silkworm chrysalis silkworm white powder without chrysalis odor and salt - Google Patents
Process for extracting edible silkworm chrysalis silkworm white powder without chrysalis odor and salt Download PDFInfo
- Publication number
- CN1091904A CN1091904A CN 93102467 CN93102467A CN1091904A CN 1091904 A CN1091904 A CN 1091904A CN 93102467 CN93102467 CN 93102467 CN 93102467 A CN93102467 A CN 93102467A CN 1091904 A CN1091904 A CN 1091904A
- Authority
- CN
- China
- Prior art keywords
- protein
- chrysalis
- smelly
- pupa
- salt
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000000034 method Methods 0.000 title claims abstract description 15
- 241000255789 Bombyx mori Species 0.000 title abstract description 12
- 239000000843 powder Substances 0.000 title abstract description 8
- 150000003839 salts Chemical class 0.000 title abstract 2
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims abstract description 26
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 25
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 25
- 239000000284 extract Substances 0.000 claims abstract description 13
- 238000001035 drying Methods 0.000 claims abstract description 9
- 230000003647 oxidation Effects 0.000 claims abstract description 6
- 238000007254 oxidation reaction Methods 0.000 claims abstract description 6
- 239000000463 material Substances 0.000 claims abstract description 5
- 241000382353 Pupa Species 0.000 claims description 20
- 230000001877 deodorizing effect Effects 0.000 claims description 15
- 238000000605 extraction Methods 0.000 claims description 12
- 239000007788 liquid Substances 0.000 claims description 8
- 239000012141 concentrate Substances 0.000 claims description 7
- 239000012528 membrane Substances 0.000 claims description 6
- 238000005238 degreasing Methods 0.000 claims description 5
- 238000005119 centrifugation Methods 0.000 claims description 4
- 102000004190 Enzymes Human genes 0.000 claims description 2
- 108090000790 Enzymes Proteins 0.000 claims description 2
- 230000005587 bubbling Effects 0.000 claims description 2
- QSHDDOUJBYECFT-UHFFFAOYSA-N mercury Chemical compound [Hg] QSHDDOUJBYECFT-UHFFFAOYSA-N 0.000 claims description 2
- 229910052753 mercury Inorganic materials 0.000 claims description 2
- 230000001954 sterilising effect Effects 0.000 claims description 2
- 238000003756 stirring Methods 0.000 claims description 2
- 238000010438 heat treatment Methods 0.000 claims 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract description 8
- 238000004332 deodorization Methods 0.000 abstract description 6
- 238000005516 engineering process Methods 0.000 abstract description 5
- 238000000926 separation method Methods 0.000 abstract description 4
- 238000010924 continuous production Methods 0.000 abstract description 3
- 230000000694 effects Effects 0.000 abstract description 3
- 238000004880 explosion Methods 0.000 abstract 1
- 239000003960 organic solvent Substances 0.000 description 6
- 238000005507 spraying Methods 0.000 description 4
- 239000005862 Whey Substances 0.000 description 3
- 102000007544 Whey Proteins Human genes 0.000 description 3
- 108010046377 Whey Proteins Proteins 0.000 description 3
- 150000002632 lipids Chemical class 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 239000002893 slag Substances 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 2
- 239000012530 fluid Substances 0.000 description 2
- 238000013467 fragmentation Methods 0.000 description 2
- 238000006062 fragmentation reaction Methods 0.000 description 2
- 239000008101 lactose Substances 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 238000000108 ultra-filtration Methods 0.000 description 2
- 238000001291 vacuum drying Methods 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 239000004677 Nylon Substances 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- 239000003245 coal Substances 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 238000005265 energy consumption Methods 0.000 description 1
- 239000012467 final product Substances 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 229920001778 nylon Polymers 0.000 description 1
- 235000019476 oil-water mixture Nutrition 0.000 description 1
- 235000019198 oils Nutrition 0.000 description 1
- 229920002492 poly(sulfone) Polymers 0.000 description 1
- 230000001376 precipitating effect Effects 0.000 description 1
- 230000002797 proteolythic effect Effects 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
Landscapes
- Peptides Or Proteins (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention discloses a technology for extracting edible silkworm chrysalis protein powder without chrysalis odor and salt. It includes the processes of defatting, grinding and separating, protein extracting, centrifugal separation, concentration, deodorization, drying, etc. And the centrifugal separation is to pump the protein extract into a DBD type disc centrifuge to obtain the protein extract. The deodorization is carried out by adopting conventional vacuum deodorization and H2O2A method combining oxidation deodorization. By adopting the method, the product quality is high, the protein content is higher than 7%, the ash content is lower than 4.5%, and the fat content is lower than 2%. The auxiliary materials (NaOH and water) can be recycled, basically has no loss, reduces the comprehensive cost and has good deodorization effect. The selected equipment is suitable for mass continuous production, and the requirements of factory equipment and fire and explosion protection are low.
Description
The present invention relates to the extraction process of pupa albumen powder, especially relate to the extraction process of the smelly salt-free edible pupa albumen powder of no pupa.
Producing the comparatively advanced method of pupa albumen powder at present is " with the method for extraction of chrysalis stinkingless protein with organic solvent " (Chinese patent, 861015118.Int.C14.C07K.3102, applying date 1986.7.4, authorize a day 1990.2.28), its technical process is: bright pupa → add water fragmentation → sieve and remove slag → drying → degreasing → deodorizing → drying → product.The protein content that this technology is produced is not less than 80%, and ash is not more than 6%, and lipid content is not more than 5%, and water content is not more than 5%.
The deficiency that this technology exists is: (1) adopts spraying drying earlier, adopts vacuum-drying again, and energy consumption is big, and vacuum-drying only is suitable for a small amount of continuous production; (2) utilize the low boiling point organic solvent precipitating proteins, and utilize this organic solvent to wash protein repeatedly with deodorizing, effect is not fairly obvious, and solvent consumption is big; (3) the ash content of coal height; (4) for guaranteeing that silkworm chrysalis is fresh, need build freezer to store the cost height.
The purpose of this invention is to provide a kind of extraction process that industrial mass production does not have the smelly salt-free edible pupa albumen powder of pupa that is suitable for.
Technical process: degreasing → defibrination separation → albumen extraction → centrifugation → concentrate → deodorizing → drying.
Degreasing: the silkworm chrysalis behind the filature is removed pelettes, contain in Nylon Bag, drop in the squeezing machine, utilize pressure to extrude pupal fat and moisture content, collect the oil-water mixture of extruding, the centrifugal pupal fat that gets.This method is removed 55% of fat approximately, as is dried pupa, then utilizes the pressure of spiral propeller, and pupal fat is pushed out.This method is removed fat 60%~70%.
Defibrination separates: the pupa after will extracting oil adds the water fragmentation, sieves to remove pupa skin pupa slag.
Albumen is extracted out: take the secondary extraction process, promptly for the first time use 0.5%NaOH, bath raio 1: 8 is heated 50~60 ℃, extracting 4 hours, incline clear liquid after, residue 2%NaOH, bath raio 1: 2 is heated 50~60 ℃, extracting is 2 hours again.Merge the extracting clear liquid twice, get protein extract.
Centrifugation: protein extract is pumped into DBD type disc centrifuge, utilize strong centrifugal force to isolate remaining fluid (merging) and remaining pupa skin pupa slag, obtain the pure protein Extract with degreasing process fluid.
Concentrate: utilize UF membrane concentration pure protein Extract.Ultra-filtration membrane is selected polysulfones for use, working pressure 5~6atm, 50~55 ℃ of service temperatures.Utilize ultra-filtration membrane can hold back the protein of macromolecule and can not hold back micromolecular NaOH, H
2The characteristic of O constantly adds dilution water, can remove NaOH and most of water fully, obtains protein concentrate Extract and UF and sees through liquid.UF is high through whey BOD in the liquid, the formation of abandoning is polluted, and (account for whey 1%) and lactose (account for whey 4~5%) have been wasted and have had high nutritive value and multi-purpose quality protein, therefore, make it again by the RO film, obtain RO concentrated solution and RO and see through liquid (promptly extremely rare alkali lye adds albumen as recycled water and extracts out in the operation).
Deodorizing: adopt traditional vacuum to drive smelly and H
2O
2The method that the oxidation deodorizing combines.UF protein concentrate Extract vacuum under 680~720mm mercury column negative pressure is driven smelly half an hour, and bubbling air adds the heavy H of 0.2~3% material
2O
2(30% content) stirs, and 20~30 ℃ of temperature controls, add an amount of H at oxidation deodorizing, sterilization half an hour
2O
2Lytic enzyme.The same RO concentrated solution of handling.
Dry: as to select spray-drier for use, 180~200 ℃ of inlet air temperature, 90~94 ℃ of temperature outs, spraying drying.
UF protein concentrate liquid is after deodorizing is handled, and spraying drying gets white to the smelly no-salt silkworm chrysalis protein powder of faint yellow no pupa.Protein content is no less than 87%, and fat is not more than 3%, and ash is no more than 1.5%.The RO concentrated solution is after deodorizing is handled, and spraying drying gets quality protein and lactose miscellany.
Compare with the method with extraction of chrysalis stinkingless protein with organic solvent, this technology has following salient feature:
1. quality product height.Protein content exceeds 7%, and ash low 4.5%.Lipid content low 2%.
2. silkworm chrysalis is only required not putrid and deteriorated getting final product, and need not build freezer and guarantee the fresh of raw material.
3. auxiliary material (NaOH and water) can be recycled after UF film and RO film, and is lossless substantially, and comprehensive cost descends.
4. good deodorization effect.The smelly composition of pupa is very complicated, it is generally acknowledged that this class material is lipid acid and proteolytic product.Because this technology removed fat in advance, the deodorizing operation adopts vacuum to drive smelly (with to utilize organic solvent to wash repeatedly with the de-odorised principle identical, all having utilized the easy volatile of stink) and H again simultaneously
2O
2The method that the oxidation deodorizing combines is held concurrently and is killed the bacterium that may bring in the production process, has eliminated hidden danger largely, and deodorizing effect is even more ideal.
5. selected equipment all is suitable for a large amount of continuous production.Factory building, equipment, fire-proof and explosion-proof require low.
And produce the silkworm chrysalis protein yield with membrane separation process and reach 50.3%, also improve, thereby can effectively utilize silkworm chrysalis protein, for large-scale developing and utilizing of silkworm chrysalis opened up wide prospect than 47.8% of organic solvent method.
Claims (2)
1, the smelly salt-free edible pupa albumen extraction process of a kind of no pupa is characterized in that it comprises degreasing, and defibrination separates, and albumen is extracted out, and centrifugation concentrates deodorizing, drying.Said centrifugation is that protein extract is pumped into DBD type disc centrifuge, obtain protein extract, concentrating is to utilize UF membrane concentration pure protein Extract, working pressure 5~6atm, 50~55 ℃ of service temperatures, utilize RO membrane concentration UF film to see through liquid again, deodorizing is to adopt traditional vacuum to drive smelly and H
2O
2The method that the oxidation deodorizing combines is driven smelly half an hour with UF protein concentrate Extract vacuum under 680~720mm mercury column negative pressure, and bubbling air adds the heavy H of 0.2~3% material
2O
2(30% content) stirs, and 20~30 ℃ of temperature controls, add an amount of H at oxidation deodorizing sterilizing half an hour
2O
2Lytic enzyme is handled the RO concentrated solution equally.
2, the smelly salt-free edible pupa albumen extraction process of a kind of no pupa according to claim 1, it is characterized in that said albumen extraction employing secondary extraction process, promptly use 0.5%NaOH bath raio 1: 8 for the first time, 50~60 ℃ of extractings 4 hours of heating, incline clear liquid after, residue is used 2%NaOH bath raio 1: 2, heats 50~60 ℃, and extracting is 2 hours again.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN 93102467 CN1091904A (en) | 1993-03-10 | 1993-03-10 | Process for extracting edible silkworm chrysalis silkworm white powder without chrysalis odor and salt |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN 93102467 CN1091904A (en) | 1993-03-10 | 1993-03-10 | Process for extracting edible silkworm chrysalis silkworm white powder without chrysalis odor and salt |
Publications (1)
Publication Number | Publication Date |
---|---|
CN1091904A true CN1091904A (en) | 1994-09-14 |
Family
ID=4984123
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN 93102467 Pending CN1091904A (en) | 1993-03-10 | 1993-03-10 | Process for extracting edible silkworm chrysalis silkworm white powder without chrysalis odor and salt |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN1091904A (en) |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN100363387C (en) * | 2005-02-28 | 2008-01-23 | 成都市裕邑丝绸有限责任公司 | Mumia protein and its production process |
CN101396059B (en) * | 2007-09-29 | 2010-12-22 | 景凯 | Production method of edible silkworm protein powder |
CN101945586A (en) * | 2008-02-13 | 2011-01-12 | 罗蒂株式会社 | The deodorizing method of collagen peptide and use the diet product or the composition of this method |
-
1993
- 1993-03-10 CN CN 93102467 patent/CN1091904A/en active Pending
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN100363387C (en) * | 2005-02-28 | 2008-01-23 | 成都市裕邑丝绸有限责任公司 | Mumia protein and its production process |
CN101396059B (en) * | 2007-09-29 | 2010-12-22 | 景凯 | Production method of edible silkworm protein powder |
CN101945586A (en) * | 2008-02-13 | 2011-01-12 | 罗蒂株式会社 | The deodorizing method of collagen peptide and use the diet product or the composition of this method |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
Köseoglu et al. | Membrane applications and research in the edible oil industry: an assessment | |
CN1133591C (en) | Membrane separation process of treating soybean processing waste water | |
CN1008684B (en) | Method for fractionation of proteins | |
US9464309B2 (en) | Methods for recovering peptides/amino acids and oil/fat from one or more protein-containing raw materials, and products produced by the methods | |
US20050106300A1 (en) | Method for producing a material having an increased solubility in alcohol | |
CN101319208B (en) | Method for preparation of bromelin | |
CA2514158A1 (en) | Enhanced production of clotting factors by cryoprecipitation | |
WO2006078823A1 (en) | Method for processing sugar cane filter cake mud and extracting component products | |
CN100535110C (en) | Novel extraction method of bromelain | |
CN106349742A (en) | Membrane treatment system for extracting corn peptide and corn yellow pigment and treatment technology | |
CN1740189A (en) | Method of extracting and separating soybean protein | |
CN1463981A (en) | Soybean segregative scleroprotein clean production method | |
JP2007166989A (en) | Method for treating bony part of fish | |
CN101333245B (en) | Method for separating human serum albumin | |
CN1091904A (en) | Process for extracting edible silkworm chrysalis silkworm white powder without chrysalis odor and salt | |
CN110551208B (en) | Method for extracting soybean trypsin inhibitor | |
CN106188183A (en) | A kind of combined extracting naringin and method of pectin from pomelo peel | |
CN109170987A (en) | A kind of digested tankage production equipment and process | |
KR20170105497A (en) | Method for fractionating components of a biomass of protein-rich microalgae | |
CN209219218U (en) | A kind of digested tankage production equipment | |
CN1378846A (en) | Shark-liver oil softgel | |
NL2028442B1 (en) | Method, steam coagulation device, and use of said device for steam coagulation of proteins from leaf juice | |
EP4118974A1 (en) | Method and system for providing an insect-based, low-fat protein meal from an insect-based raw material | |
CN1037321C (en) | Process for extracting heme and protein powder from animal blood | |
CN106801080A (en) | The method that cottonseed protein is extracted in Cottonseed Meal |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
C06 | Publication | ||
PB01 | Publication | ||
C01 | Deemed withdrawal of patent application (patent law 1993) | ||
WD01 | Invention patent application deemed withdrawn after publication |