CN106349742A - Membrane treatment system for extracting corn peptide and corn yellow pigment and treatment technology - Google Patents
Membrane treatment system for extracting corn peptide and corn yellow pigment and treatment technology Download PDFInfo
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- CN106349742A CN106349742A CN201610768031.0A CN201610768031A CN106349742A CN 106349742 A CN106349742 A CN 106349742A CN 201610768031 A CN201610768031 A CN 201610768031A CN 106349742 A CN106349742 A CN 106349742A
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- corn
- concentration
- semen maydis
- corn yellow
- enzymolysis
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
- C09B61/00—Dyes of natural origin prepared from natural sources, e.g. vegetable sources
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D61/00—Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
- B01D61/02—Reverse osmosis; Hyperfiltration ; Nanofiltration
- B01D61/08—Apparatus therefor
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D61/00—Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
- B01D61/58—Multistep processes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D65/00—Accessories or auxiliary operations, in general, for separation processes or apparatus using semi-permeable membranes
- B01D65/02—Membrane cleaning or sterilisation ; Membrane regeneration
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/18—Apparatus specially designed for the use of free, immobilized or carrier-bound enzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M45/00—Means for pre-treatment of biological substances
- C12M45/02—Means for pre-treatment of biological substances by mechanical forces; Stirring; Trituration; Comminuting
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/10—Separation or concentration of fermentation products
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biomedical Technology (AREA)
- Sustainable Development (AREA)
- Water Supply & Treatment (AREA)
- Botany (AREA)
- Mechanical Engineering (AREA)
- Gastroenterology & Hepatology (AREA)
- Biophysics (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Nanotechnology (AREA)
- General Chemical & Material Sciences (AREA)
- Separation Using Semi-Permeable Membranes (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Abstract
The invention provides a membrane treatment system for extracting a corn peptide and a corn yellow pigment. The membrane treatment system comprises a crushing device for crushing corn gluten meal, a pretreatment device for hydrolyzing protein in the gluten meal, an enzymolysis tank A, a concentration tank, an enzymolysis tank B for enzymolysis of a precipitate in the concentration tank, a centrifuging device for solid-liquid separation of enzymolysis liquid in the enzymolysis tank B, distillation equipment for distilling and extracting the corn yellow pigment from a settled substance after centrifuging, and a filtering system, wherein the filtration system comprises a ceramic membrane B and a nanofiltration system; and corn peptide concentrated liquid formed by the nanofiltration system is frozen and dried to form the corn peptide. The invention further provides a treatment technology implemented by the system; after the corn gluten meal is treated by the ceramic membrane, nanofiltration, reverse osmosis and bipolar membrane treatment system, the purity of the obtained corn peptide product reaches 95%; a product yield exceeds 85%; the quality is high; the economic benefits are significant; and the application prospect is wide.
Description
Technical field
The present invention relates to membrane treatment process, particularly to a kind of film processing system extracting Semen Maydis polypeptide and corn yellow OB
And handling process.
Background technology
China is traditional large agricultural country, and wherein Semen Maydiss are one of most important crops, is also the world three grande culture thing
One of.Investigation finds according to the study, and the maize sown area of present China has reached 2600 hectares, and its yield, more than 100,000,000 tons, occupies generation
Boundary's second, accounts for the 25% about of China's total output of grain, accounts for the 20% about of world's Semen Maydiss total output.Contain substantial amounts of in Semen Maydiss
Starch, is to produce one of optimal raw material of starch.Domestic at present is all to prepare starch using wet method, more than 80% on market
Starch is all to be produced with Semen Maydiss for raw material, often prepares 100 kilograms of Semen Maydiss of 67 kg of starch needs.Formed sediment with Maize Production
Powder, raw material is cheap, process is simple, and the purity obtaining is also high, so being one of optimal starch material.Starch industry is high at present
Speed development, has risen many starch producers both at home and abroad, has hundreds of product to be all to prepare for raw material by corn starch,
Widely inside application industry-by-industry.Because the market demand constantly increases, many corn starch producers are led to constantly to extend,
And some new producers also into corn starch industry so that Semen Maydiss sustained production increase, meanwhile, by-product zein
Powder total amount is also constantly increasing.Zein powder is mainly discharged by China at present, or processes as roughage, so not only
Waste resource, and cause environmental pollution, we also should be carried out to Zein powder while producing corn starch
Deep processing is processed, the economic worth of exploitation Zein powder.
Zein powder is to produce main by-product after starch with corn wet, is also called maize yellow-powder, corn gluten
Powder.Substantial amounts of nutrient substance is contained, than if any protein, corn yellow OB, cellulose, vitamin a and multiple in egg albumen powder
Mineral etc..Protein content therein up to 60% ~ 70%, can be divided into 4 classes, corn alcohol soluble protein (zein, 68%), paddy
Albumen (glutelin, 28%), globulin (globulins, 1.2%) and albumin (albumin).Corn yellow OB therein
Be made up of multiple pigments, including zeaxanthin (zeaxanthin, 3,3 ,-dihydroxy-beta-carotene, molecular formula is
C40h56o2), cryptoxanthine (cryptoxanthin, 3- hydroxy-beta-carotene, molecular formula is c40h56o) and phylloxanthin
(molecular formula is c40h56o2 for lutein, 3,3- dihydroxy-alpha-carotene) etc..Special odor due to Zein powder
And color, and poor taste, it is insoluble in water it is not easy to digested, and lack the necessary aminoacid needed for body, than
As tryptophan, lysine etc., thus its value very low, usually by as offal treatment or be used for feedstuff work
Industry.Carry out intensive processing with new and high technology to Zein powder to be significant, so can not only improve enterprise
Economic benefit, and being capable of environmental protection.
Polypeptide is made up of according to different arrangement modes several or several amino acids inside 20 kinds of aminoacid,
Some polypeptides have physiologic sexual function, are referred to as Functional Polypeptides.Study according to scientist and find, it is through disappearing that human body takes in protein
After changing the effect of enzyme in road, great majority are all to be absorbed in the way of small peptide, are seldom to be absorbed in the way of free amino acid.Cause
For there is certain feature polypeptide more, digested and assimilated in the form of polypeptide, the aminoacid of needed by human body can not only be supplemented,
The feature of polypeptide can be given play to, so the nutritive value of polypeptide and biological value all specific ionization aminoacid will height.Except physiological
After function, the also much excellent physicochemical property of polypeptide, such as good heat stability, preferable acid resistance, viscosity and water
Dissolubility is for the change of concentration and insensitive, and therefore polypeptide is often treated as functional factor and adds in all kinds of health foods, before development
The a piece of light of scape.Due to Zein powder aqueous phase system water-soluble is poor and some particularity abnormal smells from the patients, human body is difficult to
Absorb it, and be all made up of unbalanced aminoacid in molecule, so industrial typically pass through enzyme engineering and albumen
Matter engineering technology, by corn protein be hydrolyzed into dissolubility preferably, molecular weight, easily digested absorb and have biological function
Property Semen Maydis polypeptide, what it not only improved the dissolubility of zein, and increased other many excellent features:
(1) under the isoelectric point, IP of albumen, it is difficult to precipitate；(2) good stability；(3) there are good foaming characteristic and emulsibility；(4) dissolve
The good, high-concentration low-viscosity of property.In addition to having good physicochemical property, also there are many important features: (1) antioxygen
Change；(2) blood pressure lowering；(3) easily digest and assimilate；(4) promote the metabolism of ethanol；(5) mitigate the tired sense of body；(6) make in blood
The concentration of ammonia reduces.Therefore, also there is important meaning by hydrolyzing the Semen Maydis polypeptide obtaining to medical field.
Corn yellow OB be by phylloxanthin (c21h30o2, lutein), zeaxanthin (c40h56o2, zeaxanthin) and
The carotenoid such as cryptoxanthine (c40h50, cryptoxanthin) form, and are the natural pigment that a kind of nature is widely present,
It is the necessary class compound of people, it accounts for 0.02%-0.04% in Zein powder, i.e. 0.2 mg/g -0.37mg/g.
Corn yellow OB is a kind of pigment of pure natural, has high nutritive value, often by as natural in food industry
Toner, is also often added in some health product, and it is practical pigment by American-European numerous state approvals already, can be added to food
In product industry.China also demonstrates corn yellow OB has certain health care and trophic function to human body, and 1986 also have approved
Corn yellow OB can be used as food additive.In recent years, since, there are many functional studies for corn yellow OB, shown it
Not only there is the effects such as anti-inflammation, prevention and cure of cardiovascular disease, blood fat reducing, blood pressure lowering, mutation, but also being capable of defying age, anti-
Lipid peroxidation, anticancer, anti-cancer；More phylloxanthin, zeaxanthin and vitamin e, can reduce the general of senile cataract
Rate, prevention eye macular degeneration mainly has two kinds of extraction methods currently for the corn yellow OB in Zein powder: (1) is organic
Solvent method, including direct extraction, Hydrolyze method, with ultrasonic assistant organic solvent extraction；(2) supercritical co2 stream
Body extraction.What organic solvent used is all ordinary equipment, and extracting method is relatively easy, and the equipment ratio that supercritical technology needs
More special, troublesome poeration, separation process is comparatively laborious.In traditional extraction process, be Zein powder and organic solvent by
It is placed in the water-bath of constant temperature according to certain ratio and extracted, after extracting a period of time, centrifugation, concentrate, dry, finally
To corn yellow OB.Although this extracting method is fairly simple, also there is certain limitation, such as extraction time is longer,
Relatively more using organic solvent, lead to extraction cost higher, but extraction ratio is not high, and carry out high temperature extraction for a long time
If, it is easily caused corn yellow OB oxidized rotten, thus losing its physiologically active.
Ceramic membrane is that have loose structure with aluminium oxide, titanium oxide, zirconium oxide etc. through 1600 DEG C of high temperature sinterings
High technology ceramics filtering material.It is by the ceramic monolith of porosity 30% ~ 50%, aperture 50nm ~ 15 μm, using sol-gel process
Or the asymmetric compound film that other technique is made.Its structure usually " sandwich " formula: supporting layer (also known as carrier layer),
Transition zone (also known as intermediate layer), film layer (also known as function stratum disjunctum).The aperture of wherein supporting layer is generally 1 ~ 20 μm, and porosity is
30% ~ 65%, its effect is to increase the mechanical strength of film；The aperture of the aperture ratio supporting layer in intermediate layer is little, and its effect is to prevent film
In layer preparation process, to the infiltration of porous support layer, thickness is about 20 ~ 60 μm to granule, and porosity is 30% ~ 40%；Film layer has point
From function, aperture is about 3 ~ 10 μm from 0.8nm ~ 1 μm, thickness, and porosity is 40% ~ 55%.The pore-size distribution of whole film by
Supporting layer is gradually reduced to film layer, forms asymmetric structure distribution.Ceramic membrane can be divided into microfiltration according to aperture, and (aperture is more than
50nm), the species such as ultrafiltration (aperture 2 ~ 50nm), nanofiltration (aperture is less than 2nm).Ceramic membrane filter is a kind of " cross flow filter " shape
The fluid separation process of formula: material liquid flow at high speed in membrane tube, contain the clarified permeation liquid of small molecule component under pressure-driven
Outwards pass through film along with face vertical direction (radially), the muddy concentrated solution containing macromolecular components is rejected by, so that fluid
Reach separation, concentration, the purpose of purification.
Electric drive membrane technology refers to the membrane separating process by selective ion exchange membrane for the ion under electric field force acts on, its
Core is anion and cation exchange membrane, only allows cation to pass through using cation exchange membrane, anion exchange membrane is only permitted
Perhaps the characteristic that anion is passed through carries out the technology of Selective Separation to effects of ion.The moon under electric field force effect, in solution
There is directional migration in cation, move to another part water body from a part of water body, thus reaching solution separating, purification and concentration
Purpose.The appearance of Bipolar Membrane, makes electrodialytic purposes be expanded.Bipolar Membrane is by anion exchange membrane and cation exchange
Film is composited, and is commonly referred to intermediate catalyst layer in the middle of two-layer.Its principle is: when Bipolar Membrane both sides add voltage, molten
Ion in liquid can occur directional migration, and meanwhile hydrone can enter into intermediate catalyst layer through anion and cation exchange membrane,
There is electrolytically generated hydrion and hydroxide ion under the effect of both sides active group, charge carrier is served as with this, consumption
Water permeates to film intermediate layer further through the water in surrounding medium and is supplemented.Compared with conventional electrodialysis, in Bipolar Membrane effect
Lower water decomposition speed is enhanced more than 5 thousands of times, and therefore energy consumption substantially reduces, and electrodialytic range of application also extends significantly.Double
Pole EDBM falls within electrodialysis range of application, is by Bipolar Membrane is combined with ion exchange membrane, composition different every
So as to there is the advantage of uniqueness room (sour room, alkali room and feed liquid room) in soda acid preparation field.Under certain voltage acts on, negative and positive
Ion can pass through anion and cation exchange membrane respectively, and the hydrion being produced with Bipolar Membrane and hydroxide ion are combined and produce corresponding acid
And alkali.
Content of the invention
The technical problem to be solved is to provide a kind of film process system extracting Semen Maydis polypeptide and corn yellow OB
System, this system footprint area is little, low cost and have extraction ratio and the purity of efficient Semen Maydis polypeptide and corn yellow OB.For
This, the present invention employs the following technical solutions:
It includes reducing mechanism, for crushing maize egg albumen powder；
Pretreatment unit, for by the proteolysis in egg albumen powder；
Enzymatic vessel a, for being digested from pretreated feed liquid；
Concentration basin, is disposed of and has ceramic membranous system and concentration tank, and the enzymolysis solution of enzymatic vessel a is concentrated into through ceramic membranous system
Concentration tank precipitates；
Enzymatic vessel b, for being digested the precipitate of concentration tank；
Centrifugal treatment device, for realizing solid-liquid separation by the enzymolysis solution of enzymatic vessel b；
Distillation equipment, for by the deposit distillation extraction corn yellow OB after centrifugal treating；
Filtration system, comprises ceramic membrane b and nanofiltration system, for by the supernatant liquid filtering after centrifugal treating, through nanofiltration system
Form Semen Maydis polypeptide concentrated solution and form Semen Maydis polypeptide through lyophilization.
Further, described system also includes:
Ceramic membrane a, the supernatant of described concentration basin through ceramic membrane a permeate as syrup.
Bipolar membranous system, enters bipolar membranous system from the permeate of described nanofiltration system and carries out forming acid after a solution is processed
Alkali feed liquid, is back to ph and adjusts the Chemical cleaning with film process equipment.
Further, described Centrifugal treatment device includes centrifuge, and described centrifuge is disk centrifugal separator.
Further, described system also includes counter-infiltration system, and centrifugal treating equipment processes the deposit producing and puts into
To ethanol, after counter-infiltration system concentrates, concentrated solution is entered distillation equipment, after distillation, form corn yellow OB product.
Present invention also offers the handling process that a kind of utilization said system is realized, it comprises the following steps:
1) by Zein powder through reducing mechanism mechanical activation comminution, form superfine unqualified shape material；
2) egg albumen powder pulverized enters pretreatment unit and carries out Chemical Pretreatment operation, by adding na2so3, and insulated and stirred,
Promote the hydrolysis of protein in egg albumen powder；
3) feed liquid after hydrolyzing enters in enzymatic vessel a, adds α-amylase toward in enzymatic vessel a, adjusts optimum reaction condition, enzymolysis
After a period of time, enzymolysis solution is delivered to concentration basin；
4) as ceramic membranous system raw water box, after ceramic membranous system concentrates, concentrated solution is back to concentration tank to concentration basin, concentrates
Pot bottom precipitate enters enzymatic vessel b；
5) carry out enzymolysis processing by adding alkaline protease in enzymatic vessel b, the centrifuge by Centrifugal treatment device for the enzymolysis solution
Solid-liquid separation is realized after centrifugal treating；
6) supernatant after centrifugal treating passes through ceramic membrane b, enters back into nanofiltration system, nanofiltration concentrate after filtration treatment
For polypeptide feed liquid, after carrying out lyophilization, form Semen Maydis polypeptide product；
7) deposit that centrifugal treating produces puts into ethanol, and is concentrated with counter-infiltration system, the concentration after concentration
Liquid enters evaporation equipment, forms corn yellow OB product, counter-infiltration system permeate and evaporation condensate are ethanol, can after evaporation
Again it is back to the extraction of corn yellow OB.
Further, in step 4), ceramic membranous system permeate is syrup, can sell as product is individually outer；
In step 7), nanofiltration system permeate is saline, enters after bipolar membranous system carries out electrolysis process and forms soda acid material
Liquid, is back to ph in technique and adjusts the Chemical cleaning with film process equipment.
Further, in step 3), the concentration of described α-amylase is 1.0 ~ 1.2%；
Hydrolysis temperature controls at 80 ~ 85 DEG C；
Enzymolysis ph controls 6.0 ~ 6.5；
Enzymolysis time controls in 50 ~ 60min.
Further, in step 5), the optimal ph of enzymolysis of described alkaline protease is 9 ~ 9.5；
Optimal concentration of substrate should control 10 ~ 12%；
Hydrolysis temperature should control at 45 ~ 50 DEG C.
Further, in step 7), described counter-infiltration system uses tubular type or disc tube reverse osmosis (dt-ro) membrane module；
The average operating pressure of described counter-infiltration system is 1.0 ~ 1.5mpa；
The average flux of described counter-infiltration system is 25 ~ 30lmh；
The response rate of described counter-infiltration system is 80 ~ 85%.
Further, described Zein powder needs to carry out pulverization process before recycling treatment, reach 200 after process ~
250 mesh.
Further, enter Chemical Pretreatment operation after described Zein powder pulverization process, by chemical treatment side
Formula opens the middle hydrophobic bond of protein, disulfide bond and hydrogen bond, destroys the multilevel hierarchy of protein；
Preferably, Chemical Pretreatment selects medicament is na2so3；
Preferably, na2so3Add concentration be 1.5 ~ 2.0g/l；
Preferably, pretreatment controls temperature to be 40 ~ 50 DEG C.
Further, the enzymolysis solution that enzymatic vessel a produces enters concentration basin, carries out concentration by ceramic membrane a, produces two
Syrupy product based on sugar is it is preferred that ceramic membrane filter aperture is 20 ~ 30nm.
Further, the membrane flux of described nanofiltration system is 15lmh, and the system response rate is 80%.
Further, Bipolar Membrane carries out electrolysis process it is preferred that operation electric current density is to nanofiltration system permeate
0.2a/cm2, final salt room feed liquid salinity is less than 0.05%.
Further, the deposit that centrifugation apparatus produce enters counter-infiltration system, by reverse osmosis after alcohol steep
The evaporation of the concentration of permeable system and distillation equipment obtains corn yellow OB, and counter-infiltration system permeate and distillation equipment condensed fluid are
Ethanol, is back to extracting technology again it is preferred that the concentration of alcohol solvent is 95%.
The present invention has the advantage that as follows:
1) pass through the ceramic membrane a processing system of the present invention, eliminate a large amount of two sugar substances, define two sugar products (syrup), carry
The high economy of whole set process；
2) pass through the ceramic membrane b processing system of the present invention, in dialysis solution, macromolecular substances significantly reduce, viscosity reduces, nanofiltration system
The system cleaning frequency extends；
3) ceramic film processing system floor space is less than using settler, and automaticity improves, and cost of labor reduces；
4) ceramic membranous system is resistant to the high temperature of enzymolysis solution, corrosion-resistant, resistant is blocked up；
5) reduction of feed volume can be greatly reduced by the nanofiltration technique of the present invention, reduce investment and the operating cost of lyophilization system；
6) salinity in polypeptide feed liquid can be removed by the nanofiltration system of the present invention, reduced product salt content, Jin Erti
The quality of high Semen Maydis polypeptide；
7) by the freeze drying process of the present invention it is ensured that the high activity of product；
8) salinity of removing can be carried out by electrolysis antacid alkali by the bipolar membranous system of the present invention and again be back to production, realize useless
The resource reuse of liquid；
9) by the counter-infiltration system of the present invention, distillation equipment investment and operating cost can be reduced, and achieve alcohol solvent
Reclaim；
10) by the ceramic membrane+nanofiltration+reverse osmosiss+bipolar film processing system of the present invention, Zein powder is processed, obtained
The Semen Maydis polypeptide product purity obtaining is up to 95%, and, more than 85%, quality is high, remarkable in economical benefits for product yield；
11) handling process being provided by the present invention is carried out deep processing to Zein powder and is with a wide range of applications, not only
Carry out comprehensive utilization of resources, also develop the product of high value simultaneously.
Brief description
Fig. 1 is the schematic diagram of the present invention.
Specific embodiment
Refer to the attached drawing.
Embodiment one: system of the present invention includes system and includes:
Reducing mechanism, for crushing maize egg albumen powder；Pretreatment unit, for by the proteolysis in egg albumen powder；Enzymatic vessel
A, for being digested from pretreated feed liquid；Concentration basin, is disposed of and has ceramic membranous system and concentration tank, enzymatic vessel
The enzymolysis solution of a is concentrated into concentration tank precipitation through ceramic membranous system；Enzymatic vessel b, for being digested the precipitate of concentration tank；
Centrifugal treatment device, for realizing solid-liquid separation by the enzymolysis solution of enzymatic vessel b；Distillation equipment, for will be heavy after centrifugal treating
Starch matter distillation extraction corn yellow OB；
Filtration system, comprises ceramic membrane b and nanofiltration system, for by the supernatant liquid filtering after centrifugal treating, through nanofiltration system
Form Semen Maydis polypeptide concentrated solution and form Semen Maydis polypeptide through lyophilization.
Embodiment two: the present invention also provides handling process to comprise the following steps:
1) by Zein powder through reducing mechanism mechanical activation comminution, form superfine unqualified shape material；
2) egg albumen powder pulverized enters pretreatment unit and carries out Chemical Pretreatment operation, by adding na2so3, and insulated and stirred,
Promote the hydrolysis of protein in egg albumen powder；
3) feed liquid after hydrolyzing enters in enzymatic vessel a, adds α-amylase toward in enzymatic vessel a, adjusts optimum reaction condition, enzymolysis
After a period of time, enzymolysis solution is delivered to concentration basin；
4) as ceramic membranous system raw water box, after ceramic membranous system concentrates, concentrated solution is back to concentration tank to concentration basin, concentrates
Pot bottom precipitate enters enzymatic vessel b；
5) carry out enzymolysis processing by adding alkaline protease in enzymatic vessel b, the centrifuge by Centrifugal treatment device for the enzymolysis solution
Solid-liquid separation is realized after centrifugal treating；
6) supernatant after centrifugal treating passes through ceramic membrane b, enters back into nanofiltration system, nanofiltration concentrate after filtration treatment
For polypeptide feed liquid, after carrying out lyophilization, form Semen Maydis polypeptide product；
7) deposit that centrifugal treating produces puts into ethanol, and is concentrated with counter-infiltration system, the concentration after concentration
Liquid enters evaporation equipment, forms corn yellow OB product, counter-infiltration system permeate and evaporation condensate are ethanol, can after evaporation
Again it is back to the extraction of corn yellow OB.
Further, in step 4), ceramic membranous system permeate is syrup, can sell as product is individually outer；
In step 7), nanofiltration system permeate is saline, enters after bipolar membranous system carries out electrolysis process and forms soda acid material
Liquid, is back to ph in technique and adjusts the Chemical cleaning with film process equipment.
Further, in step 3), the concentration of described α-amylase is 1.0 ~ 1.2%；
Hydrolysis temperature controls at 80 ~ 85 DEG C；
Enzymolysis ph controls 6.0 ~ 6.5；
Enzymolysis time controls in 50 ~ 60min.
Further, in step 5), the optimal ph of enzymolysis of described alkaline protease is 9 ~ 9.5；
Optimal concentration of substrate should control 10 ~ 12%；
Hydrolysis temperature should control at 45 ~ 50 DEG C.
Further, in step 7), described counter-infiltration system uses tubular type or disc tube reverse osmosis (dt-ro) membrane module；
The average operating pressure of described counter-infiltration system is 1.0 ~ 1.5mpa；
The average flux of described counter-infiltration system is 25 ~ 30lmh；
The response rate of described counter-infiltration system is 80 ~ 85%.
Further, described Zein powder needs to carry out pulverization process before recycling treatment, reach 200 after process ~
250 mesh.
Further, enter Chemical Pretreatment operation after described Zein powder pulverization process, by chemical treatment side
Formula opens the middle hydrophobic bond of protein, disulfide bond and hydrogen bond, destroys the multilevel hierarchy of protein；
Preferably, Chemical Pretreatment selects medicament is na2so3；
Preferably, na2so3Add concentration be 1.5 ~ 2.0g/l；
Preferably, pretreatment controls temperature to be 40 ~ 50 DEG C.
Further, the enzymolysis solution that enzymatic vessel a produces enters concentration basin, carries out concentration by ceramic membrane a, produces two
Syrupy product based on sugar is it is preferred that ceramic membrane filter aperture is 20 ~ 30nm.
Further, the membrane flux of described nanofiltration system is 15lmh, and the system response rate is 80%.
Further, Bipolar Membrane carries out electrolysis process it is preferred that operation electric current density is to nanofiltration system permeate
0.2a/cm2, final salt room feed liquid salinity is less than 0.05%.
Further, the deposit that centrifugation apparatus produce enters counter-infiltration system, by reverse osmosis after alcohol steep
The evaporation of the concentration of permeable system and distillation equipment obtains corn yellow OB, and counter-infiltration system permeate and distillation equipment condensed fluid are
Ethanol, is back to extracting technology again it is preferred that the concentration of alcohol solvent is 95%.
Claims (10)
1. the film processing system of a kind of extraction Semen Maydis polypeptide and corn yellow OB is it is characterised in that this system includes:
Reducing mechanism, for crushing maize egg albumen powder；
Pretreatment unit, for by the proteolysis in egg albumen powder；
Enzymatic vessel a, for being digested from pretreated feed liquid；
Concentration basin, is disposed of and has ceramic membranous system and concentration tank, and the enzymolysis solution of enzymatic vessel a is concentrated into through ceramic membranous system
Concentration tank precipitates；
Enzymatic vessel b, for being digested the precipitate of concentration tank；
Centrifugal treatment device, for realizing solid-liquid separation by the enzymolysis solution of enzymatic vessel b；
Distillation equipment, for by the deposit distillation extraction corn yellow OB after centrifugal treating；
Filtration system, comprises ceramic membrane b and nanofiltration system, for by the supernatant liquid filtering after centrifugal treating, through nanofiltration system
Form Semen Maydis polypeptide concentrated solution and form Semen Maydis polypeptide through lyophilization.
2. a kind of film processing system extracting Semen Maydis polypeptide and corn yellow OB according to claim 1 it is characterised in that
It also includes:
Ceramic membrane a, the supernatant of described concentration basin through ceramic membrane a permeate as syrup.
3. a kind of film processing system extracting Semen Maydis polypeptide and corn yellow OB according to claim 1 it is characterised in that
It also includes:
Bipolar membranous system, enters bipolar membranous system from the permeate of described nanofiltration system and carries out forming soda acid material after a solution is processed
Liquid, is back to ph and adjusts the Chemical cleaning with film process equipment.
4. a kind of film processing system extracting Semen Maydis polypeptide and corn yellow OB according to claim 1 it is characterised in that
Described Centrifugal treatment device includes centrifuge, and described centrifuge is disk centrifugal separator.
5. a kind of film processing system extracting Semen Maydis polypeptide and corn yellow OB according to claim 1 it is characterised in that
It also includes counter-infiltration system, and centrifugal treating equipment processes the deposit producing and puts into ethanol, through counter-infiltration system
After concentration, concentrated solution is entered distillation equipment, after distillation, form corn yellow OB product.
6. the film extracting Semen Maydis polypeptide and corn yellow OB realized using described film processing system as arbitrary in claim 1-5
Handling process is it is characterised in that described technique comprises the following steps:
By Zein powder through reducing mechanism mechanical activation comminution, form superfine unqualified shape material；
The egg albumen powder pulverized enters pretreatment unit and carries out Chemical Pretreatment operation, by adding na2so3, and insulated and stirred, promote
Make the hydrolysis of protein in egg albumen powder；
Feed liquid after hydrolysis enters in enzymatic vessel a, adds α-amylase toward in enzymatic vessel a, adjusts optimum reaction condition, digests one
After the section time, enzymolysis solution is delivered to concentration basin；
As ceramic membranous system raw water box, after ceramic membranous system concentrates, concentrated solution is back to concentration tank, concentration tank to concentration basin
Bottom sediment enters enzymatic vessel b；
Carry out enzymolysis processing by adding alkaline protease in enzymatic vessel b, enzymolysis solution by the centrifuge of Centrifugal treatment device from
The heart realizes solid-liquid separation after processing；
Supernatant after centrifugal treating passes through ceramic membrane b, enters back into nanofiltration system, nanofiltration concentrate is after filtration treatment
Polypeptide feed liquid, forms Semen Maydis polypeptide product after carrying out lyophilization；
7) deposit that centrifugal treating produces puts into ethanol, and is concentrated with counter-infiltration system, the concentration after concentration
Liquid enters evaporation equipment, forms corn yellow OB product, counter-infiltration system permeate and evaporation condensate are ethanol, can after evaporation
Again it is back to the extraction of corn yellow OB.
7. a kind of membrane treatment process extracting Semen Maydis polypeptide and corn yellow OB according to claim 6 it is characterised in that
In step 4), ceramic membranous system permeate is syrup, can sell as product is individually outer；
In step 7), nanofiltration system permeate is saline, enters after bipolar membranous system carries out electrolysis process and forms soda acid material
Liquid, is back to ph in technique and adjusts the Chemical cleaning with film process equipment.
8. a kind of membrane treatment process extracting Semen Maydis polypeptide and corn yellow OB according to claim 6 it is characterised in that
In step 3), the concentration of described α-amylase is 1.0 ~ 1.2%；
Hydrolysis temperature controls at 80 ~ 85 DEG C；
Enzymolysis ph controls 6.0 ~ 6.5；
Enzymolysis time controls in 50 ~ 60min.
9. a kind of membrane treatment process extracting Semen Maydis polypeptide and corn yellow OB according to claim 6 it is characterised in that
In step 5), the optimal ph of enzymolysis of described alkaline protease is 9 ~ 9.5；
Optimal concentration of substrate should control 10 ~ 12%；
Hydrolysis temperature should control at 45 ~ 50 DEG C.
10. a kind of membrane treatment process extracting Semen Maydis polypeptide and corn yellow OB according to claim 6 it is characterised in that
In step 7), described counter-infiltration system uses tubular type or disc tube reverse osmosis (dt-ro) membrane module；
The average operating pressure of described counter-infiltration system is 1.0 ~ 1.5mpa；
The average flux of described counter-infiltration system is 25 ~ 30lmh；
The response rate of described counter-infiltration system is 80 ~ 85%.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109652386A (en) * | 2019-01-03 | 2019-04-19 | 重庆工商大学 | A method of enzyme is recycled in catalysis reaction |
| CN110102187A (en) * | 2018-04-27 | 2019-08-09 | 轻工业环境保护研究所 | The method and device of concentrated water catalytic ozonation on-line cleaning reverse osmosis/nanofiltration membrane |
| CN110241165A (en) * | 2019-07-03 | 2019-09-17 | 齐鲁工业大学 | A method of luteole is extracted from corn protein powder enzymatic hydrolysis jelly |
| CN110256319A (en) * | 2019-07-03 | 2019-09-20 | 齐鲁工业大学 | A method of extracting luteole from the corn protein powder of enzymatic hydrolysis |
| CN112680491A (en) * | 2019-10-17 | 2021-04-20 | 江南大学 | Method for preparing high-F-value oligopeptide from maize yellow powder |
| CN114057314A (en) * | 2020-07-29 | 2022-02-18 | 三达膜科技（厦门）有限公司 | Method for recycling wastewater in corn deep processing process |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110102187A (en) * | 2018-04-27 | 2019-08-09 | 轻工业环境保护研究所 | The method and device of concentrated water catalytic ozonation on-line cleaning reverse osmosis/nanofiltration membrane |
| CN109652386A (en) * | 2019-01-03 | 2019-04-19 | 重庆工商大学 | A method of enzyme is recycled in catalysis reaction |
| CN109652386B (en) * | 2019-01-03 | 2022-04-12 | 重庆工商大学 | Method for recycling enzyme in catalytic reaction |
| CN110241165A (en) * | 2019-07-03 | 2019-09-17 | 齐鲁工业大学 | A method of luteole is extracted from corn protein powder enzymatic hydrolysis jelly |
| CN110256319A (en) * | 2019-07-03 | 2019-09-20 | 齐鲁工业大学 | A method of extracting luteole from the corn protein powder of enzymatic hydrolysis |
| CN110241165B (en) * | 2019-07-03 | 2021-05-18 | 齐鲁工业大学 | Method for extracting zeaxanthin from corn gluten meal enzymolysis jelly |
| CN112680491A (en) * | 2019-10-17 | 2021-04-20 | 江南大学 | Method for preparing high-F-value oligopeptide from maize yellow powder |
| CN114057314A (en) * | 2020-07-29 | 2022-02-18 | 三达膜科技（厦门）有限公司 | Method for recycling wastewater in corn deep processing process |
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