CN109172945A - It is a kind of to go wrinkle and autogenous cell regeneration method using subcutaneous injection skin by different level - Google Patents
It is a kind of to go wrinkle and autogenous cell regeneration method using subcutaneous injection skin by different level Download PDFInfo
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- CN109172945A CN109172945A CN201811227386.4A CN201811227386A CN109172945A CN 109172945 A CN109172945 A CN 109172945A CN 201811227386 A CN201811227386 A CN 201811227386A CN 109172945 A CN109172945 A CN 109172945A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M5/00—Devices for bringing media into the body in a subcutaneous, intra-vascular or intramuscular way; Accessories therefor, e.g. filling or cleaning devices, arm-rests
- A61M5/178—Syringes
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/14—Blood; Artificial blood
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/14—Blood; Artificial blood
- A61K35/16—Blood plasma; Blood serum
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M5/00—Devices for bringing media into the body in a subcutaneous, intra-vascular or intramuscular way; Accessories therefor, e.g. filling or cleaning devices, arm-rests
- A61M5/42—Devices for bringing media into the body in a subcutaneous, intra-vascular or intramuscular way; Accessories therefor, e.g. filling or cleaning devices, arm-rests having means for desensitising skin, for protruding skin to facilitate piercing, or for locating point where body is to be pierced
- A61M5/427—Locating point where body is to be pierced, e.g. vein location means using ultrasonic waves, injection site templates
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
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Abstract
Wrinkle and autogenous cell regeneration method are gone using subcutaneous injection skin by different level the invention discloses a kind of, the blood of 9cc is extracted from patient's body including doctor, and blood is put into time in speed change centrifugation apparatus and carries out centrifugation extraction, generate the autoblood product for being rich in high concentration living cells, doctor takes out blood product out of centrifugation apparatus, doctor is extracted PRP and CD34+ cell using syringe from blood product, it is mixed into concentration growth factor injection, the platelet rich plasma in blood product is extracted be used as living cells injection again by user.The present invention out of blood samples of patients by extracting blood, and extract platelet rich plasma, CD34+ cell respectively out of blood using centrifugation apparatus, CGF injection, living cells injection, sodium hyaluronate injection and collagen injection are mixed into medicament, inject patient's body, promote patient skin cytothesis so as to play, the effect eliminated wrinkle of skin, improve skin mechanism, makes the rejuvenation of skin mechanism.
Description
Technical field
The present invention relates to a kind of skins to go wrinkle and autogenous cell regeneration method, in particular to a kind of utilization is subcutaneous by different level
Injection skin goes wrinkle and autogenous cell regeneration method.
Background technique
It is well known that human body is as time goes by, Cell division in vivo number is reduced, and skin is aoxidized for a long time, be cannot get
Good maintenance leads to the decline of internal cell APSC pluripotent cell with age, and dead skin will be attached to skin table
Face and do not fall off, cause skin surface the bad performance such as wrinkle, aging occur, cause a series of problems, seriously affect beauty.
Summary of the invention
The technical problem to be solved by the present invention is to overcome the defects of the prior art, provide a kind of utilize and are subcutaneously injected by different level
Skin goes wrinkle and autogenous cell regeneration method.
In order to solve the above-mentioned technical problems, the present invention provides the following technical solutions:
The present invention is a kind of to go wrinkle and autogenous cell regeneration method using subcutaneous injection skin by different level, specifically includes following step
It is rapid:
A. doctor extracts the blood of 9cc from patient's body, and blood is put into time in speed change centrifugation apparatus and carries out centrifugation extraction,
Blood platelet is sufficiently activated using physical acceleration and deceleration, generates the autoblood product for being rich in high concentration living cells,
After the completion of extraction, doctor takes out blood product out of centrifugation apparatus, successively includes 2ml platelet poor plasma in blood product
(PPP), the red blood cell (RBC) of 2ml platelet rich plasma (PRP), the CD34+ cell of 0.6ml and 5ml or so, doctor is by rich blood
Platelet-poor plasma is divided into the blood plasma of 2 parts of equal portions, and a copy of it is put into blood separator and is separated, and it is living to isolate autoblood
Cell, doctor reuses syringe, and by P, another RP and CD34+ cell is extracted from blood product, is mixed into concentration growth
The factor (CGF) injection, user is again using the autoblood living cells extracted out of platelet rich plasma as living cells needle
Agent；
B. doctor is carried out disinfection by skin surface of the alcohol to patient, is reused mark pen and is carried out at the injection site of patient
Uniform mark, after the completion of mark, doctor takes out syringe out of packaging, and the air in syringe is released, injection is reused
Device is in 45 ° of angle insertion sodium hyaluronate injection bottle, doctor is by way of slowly pulling back by sodium hyaluronate injection inhalation syringe
It is interior, while syringe being avoided to draw air into, further according to aforesaid operations by collagen injection, CGF injection and living cells injection
Respectively in inhalation syringe；
C. in injection process, doctor enters syringe needle point and the skin of patient injection site at an angle, generally
15-45 °, doctor is gently helped syringe needle using left hand and syringe needle is kept to fix, and syringe needle point is pushed into the skin of patient by the right hand,
When injecting for the first time, by the hypodermis layer of needle point push-in patient skin, and it is pushed into medicament on a small quantity using syringe, is cured after injection
Life again steadily extracts needle point, is moved to another mark point, needle point is pushed into patient skin according to above-mentioned gimmick, and this time
It is more last shallow to inject level, same a small amount of push-in confection, then needle point is extracted, in subsequent injection process, doctor
By tip alignment mark point, and the more last injection level of per injection level is shallow, injects a small amount of medicament every time, until medicine
Agent is all injected into patient's body；
D. doctor carries out homogeneously kneaded to the injection site of patient after injection, after the completion of massage, before doctor's using face
The mark line of patient injection site is wiped after immersing alcohol, and is applied at injection pinprick using erythromycin ophthalmic ointment, is being smeared
After the completion, doctor further takes out ice bag, carries out ice compress using injection site of the ice bag to patient, the time is 5-10 minutes or so.
Compared with prior art, beneficial effects of the present invention are as follows:
1: the present invention extracts rich platelet by extracting blood out of blood samples of patients, and using centrifugation apparatus respectively out of blood
Blood plasma, CD34+ cell, while extracting living cells using blood separator out of platelet rich plasma again, by by Fu Xue little
Plate blood plasma and CD34+ mixing with cells are at CGF injection, by CGF injection, living cells injection, sodium hyaluronate injection and collagen injection
It is mixed into medicament, injects patient's body, promotes patient skin cytothesis so as to play, wrinkle of skin is eliminated, improves skin
The effect of mechanism makes the rejuvenation of skin mechanism.
2: the present invention in patient skin by injecting living cells injection, so as to utilize living cells activation patient's body
Blood platelet after the secretion of platelet being activated goes out growth factor and imports stem cell, stimulates the fibroblast of surrounding, generates bone
The elastomers such as collagen or elastin, nearby produce new vascular tissue, promote Skin Cell to regenerate with this, and it is living to shine cell
Power.
3: the present invention out of blood samples of patients simultaneously by extracting platelet rich plasma and CD34+ cell, by rich platelet
CGF growth factor made of blood plasma, the combination of CD34+ cell injects patient's body, sustainable promotion local skin and subcutaneous tissue
Regeneration plays firm skin, lightens the stain, pore refining, eliminates the effects of wrinkle of skin.
Specific embodiment
Preferred embodiment of the invention below is illustrated, it should be understood that preferred embodiment described herein is only used for
The description and interpretation present invention, is not intended to limit the present invention.
Embodiment 1
The present invention provide it is a kind of using by different level subcutaneous injection skin go wrinkle and autogenous cell regeneration method, specifically include as follows
Step:
A. doctor extracts the blood of 9cc from patient's body, and blood is put into time in speed change centrifugation apparatus and carries out centrifugation extraction,
Blood platelet is sufficiently activated using physical acceleration and deceleration, generates the autoblood product for being rich in high concentration living cells,
After the completion of extraction, doctor takes out blood product out of centrifugation apparatus, successively includes 2ml platelet poor plasma in blood product
(PPP), the red blood cell (RBC) of 2ml platelet rich plasma (PRP), the CD34+ cell of 0.6ml and 5ml or so, doctor is by rich blood
Platelet-poor plasma is divided into the blood plasma of 2 parts of equal portions, and a copy of it is put into blood separator and is separated, and it is living to isolate autoblood
Cell, doctor reuses syringe, and by P, another RP and CD34+ cell is extracted from blood product, is mixed into concentration growth
The factor (CGF) injection, user is again using the autoblood living cells extracted out of platelet rich plasma as living cells needle
Agent；
B. doctor is carried out disinfection by skin surface of the alcohol to patient, is reused mark pen and is carried out at the injection site of patient
Uniform mark, after the completion of mark, doctor takes out syringe out of packaging, and the air in syringe is released, injection is reused
Device is in 45 ° of angle insertion sodium hyaluronate injection bottle, doctor is by way of slowly pulling back by sodium hyaluronate injection inhalation syringe
It is interior, while syringe being avoided to draw air into, further according to aforesaid operations by collagen injection, CGF injection and living cells injection
Respectively in inhalation syringe；
C. in injection process, doctor enters syringe needle point and the skin of patient injection site at an angle, generally
15-45 °, doctor is gently helped syringe needle using left hand and syringe needle is kept to fix, and syringe needle point is pushed into the skin of patient by the right hand,
When injecting for the first time, by the hypodermis layer of needle point push-in patient skin, and it is pushed into medicament on a small quantity using syringe, is cured after injection
Life again steadily extracts needle point, is moved to another mark point, needle point is pushed into patient skin according to above-mentioned gimmick, and this time
It is more last shallow to inject level, same a small amount of push-in confection, then needle point is extracted, in subsequent injection process, doctor
By tip alignment mark point, and the more last injection level of per injection level is shallow, injects a small amount of medicament every time, until medicine
Agent is all injected into patient's body；
D. doctor carries out homogeneously kneaded to the injection site of patient after injection, after the completion of massage, before doctor's using face
The mark line of patient injection site is wiped after immersing alcohol, and is applied at injection pinprick using erythromycin ophthalmic ointment, is being smeared
After the completion, doctor further takes out ice bag, carries out ice compress using injection site of the ice bag to patient, the time is 5-10 minutes or so.
Specifically, doctor extracts the blood of 9cc by the method for blood drawing from patient's body, and blood is put into speed change in time
Centrifugation extraction is carried out in centrifugation apparatus, is sufficiently activated blood platelet using physical acceleration and deceleration, is generated and be rich in high concentration
The autoblood product of living cells, after the completion of extraction, doctor takes out blood product out of centrifugation apparatus, in blood product according to
Secondary includes the red of 2ml platelet poor plasma (PPP), 2ml platelet rich plasma (PRP), the CD34+ cell of 0.6ml and 5ml or so
Platelet rich plasma is divided into the blood plasma of 2 parts of equal portions by cell (RBC), doctor, and a copy of it is put into blood separator and is carried out
Separation, isolates autoblood living cells, and doctor reuses syringe for P another RP and CD34+ cell from blood product
It extracts, is mixed into concentration growth factor (CGF) injection, the self blood that user will extract out of platelet rich plasma again
Liquid living cells is carried out disinfection as living cells injection, doctor by skin surface of the alcohol to patient, is reused mark pen and is being suffered from
Uniform mark is carried out at the injection site of person, after the completion of mark, doctor takes out syringe out of packaging, by the sky in syringe
Gas is released, and reuses syringe so that in 45 ° of angle insertion sodium hyaluronate injection bottle, doctor urinates glass by way of slowly pulling back
In sour injection inhalation syringe, while syringe being avoided to draw air into, further according to aforesaid operations by collagen injection, CGF
In injection and living cells injection difference inhalation syringe, in injection process, doctor is by syringe needle point and patient injection site
Skin enter at an angle, generally 15-45 °, doctor is gently helped syringe needle using left hand and syringe needle is kept to fix, the right hand will infuse
Emitter needle point is pushed into the skin of patient, when injecting first time, by the hypodermis layer of needle point push-in patient skin, and is used
Syringe is pushed into medicament on a small quantity, and doctor again steadily extracts needle point after injection, another mark point is moved to, according to above-mentioned gimmick
Needle point is pushed into patient skin, and this time injection level is more last shallow, same a small amount of push-in confection, then needle point is pulled out
Out, in subsequent injection process, doctor is by tip alignment mark point, and the more last injection level of per injection level
Shallowly, a small amount of medicament is injected every time, until medicament is all injected into patient's body, injection of the doctor to patient after injection
Position carries out homogeneously kneaded, wipes the mark line of patient injection site after alcohol is immersed after the completion of massage, before doctor's using face
It removes, and is applied at injection pinprick using erythromycin ophthalmic ointment, after the completion of smearing, doctor further takes out ice bag, using ice bag to trouble
The injection site of person carries out ice compress, and the time is 5-10 minutes or so.
In conclusion the present invention is mentioned out of blood respectively by extracting blood out of blood samples of patients, and using centrifugation apparatus
Platelet rich plasma, CD34+ cell are taken out, while extracting living cells using blood separator out of platelet rich plasma again,
By by platelet rich plasma and CD34+ mixing with cells at CGF injection, by CGF injection, living cells injection, sodium hyaluronate injection and
Collagen injection is mixed into medicament, injects patient's body, promotes patient skin cytothesis so as to play, and eliminates skin wrinkle
Line, the effect for improving skin mechanism, make the rejuvenation of skin mechanism；By injecting living cells injection in patient skin, so as to
Utilize the blood platelet of living cells activation patient's body, after the secretion of platelet being activated goes out growth factor and imports stem cell, thorn
Fibroblast around swashing is generated the elastomers such as ossein or elastin, nearby produces new vascular tissue, promoted with this
It is regenerated into Skin Cell, shines cell viability；It is thin by extracting platelet rich plasma and CD34+ out of blood samples of patients simultaneously
CGF growth factor made of platelet rich plasma, the combination of CD34+ cell is injected patient's body, the local skin of sustainable promotion by born of the same parents
Skin and subcutaneous tissue regeneration play firm skin, lighten the stain, pore refining, eliminate the effects of wrinkle of skin.
Finally, it should be noted that the foregoing is only a preferred embodiment of the present invention, it is not intended to restrict the invention,
Although the present invention is described in detail referring to the foregoing embodiments, for those skilled in the art, still may be used
To modify the technical solutions described in the foregoing embodiments or equivalent replacement of some of the technical features.
All within the spirits and principles of the present invention, any modification, equivalent replacement, improvement and so on should be included in of the invention
Within protection scope.
Claims (1)
1. a kind of go wrinkle and autogenous cell regeneration method using subcutaneous injection skin by different level, which is characterized in that the skin
Wrinkle and autogenous cell regeneration method are gone, is specifically comprised the following steps:
A. doctor extracts the blood of 9cc from patient's body, and blood is put into time in speed change centrifugation apparatus and carries out centrifugation extraction,
Blood platelet is sufficiently activated using physical acceleration and deceleration, generates the autoblood product for being rich in high concentration living cells,
After the completion of extraction, doctor takes out blood product out of centrifugation apparatus, successively includes 2ml platelet poor plasma in blood product
(PPP), the red blood cell (RBC) of 2ml platelet rich plasma (PRP), the CD34+ cell of 0.6ml and 5ml or so, doctor is by rich blood
Platelet-poor plasma is divided into the blood plasma of 2 parts of equal portions, and a copy of it is put into blood separator and is separated, and it is living to isolate autoblood
Cell, doctor reuses syringe, and by P, another RP and CD34+ cell is extracted from blood product, is mixed into concentration growth
The factor (CGF) injection, user is again using the autoblood living cells extracted out of platelet rich plasma as living cells needle
Agent；
B. doctor is carried out disinfection by skin surface of the alcohol to patient, is reused mark pen and is carried out at the injection site of patient
Uniform mark, after the completion of mark, doctor takes out syringe out of packaging, and the air in syringe is released, injection is reused
Device is in 45 ° of angle insertion sodium hyaluronate injection bottle, doctor is by way of slowly pulling back by sodium hyaluronate injection inhalation syringe
It is interior, while syringe being avoided to draw air into, further according to aforesaid operations by collagen injection, CGF injection and living cells injection
Respectively in inhalation syringe；
C. in injection process, doctor enters syringe needle point and the skin of patient injection site at an angle, generally
15-45 °, doctor is gently helped syringe needle using left hand and syringe needle is kept to fix, and syringe needle point is pushed into the skin of patient by the right hand,
When injecting for the first time, by the hypodermis layer of needle point push-in patient skin, and it is pushed into medicament on a small quantity using syringe, is cured after injection
Life again steadily extracts needle point, is moved to another mark point, needle point is pushed into patient skin according to above-mentioned gimmick, and this time
It is more last shallow to inject level, same a small amount of push-in confection, then needle point is extracted, in subsequent injection process, doctor
By tip alignment mark point, and the more last injection level of per injection level is shallow, injects a small amount of medicament every time, until medicine
Agent is all injected into patient's body；
D. doctor carries out homogeneously kneaded to the injection site of patient after injection, after the completion of massage, before doctor's using face
The mark line of patient injection site is wiped after immersing alcohol, and is applied at injection pinprick using erythromycin ophthalmic ointment, is being smeared
After the completion, doctor further takes out ice bag, carries out ice compress using injection site of the ice bag to patient, the time is 5-10 minutes or so.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811227386.4A CN109172945A (en) | 2018-10-22 | 2018-10-22 | It is a kind of to go wrinkle and autogenous cell regeneration method using subcutaneous injection skin by different level |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811227386.4A CN109172945A (en) | 2018-10-22 | 2018-10-22 | It is a kind of to go wrinkle and autogenous cell regeneration method using subcutaneous injection skin by different level |
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| Publication Number | Publication Date |
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| CN109172945A true CN109172945A (en) | 2019-01-11 |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101541951A (en) * | 2006-10-04 | 2009-09-23 | 国立大学法人东京大学 | Structure enclosing hematopoietic progenitor cells from ES cells and method for preparing blood cells using the same |
| CN105567630A (en) * | 2016-01-14 | 2016-05-11 | 齐湘杰 | Kit for synchronously separating cord blood PRP, cord blood plasma and cord blood cells |
| CN106029102A (en) * | 2015-01-15 | 2016-10-12 | 小威廉·K·博斯 | Repair and rejuvenation of tissues using platelet-rich plasma |
| CN107281467A (en) * | 2017-05-22 | 2017-10-24 | 胡建红 | A kind of preparation method of cell factor for autogenous cell skin regeneration |
| US20180094244A1 (en) * | 2015-02-18 | 2018-04-05 | Enlivex Therapeutics Ltd. | Combination immune therapy and cytokine control therapy for cancer treatment |
-
2018
- 2018-10-22 CN CN201811227386.4A patent/CN109172945A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101541951A (en) * | 2006-10-04 | 2009-09-23 | 国立大学法人东京大学 | Structure enclosing hematopoietic progenitor cells from ES cells and method for preparing blood cells using the same |
| CN106029102A (en) * | 2015-01-15 | 2016-10-12 | 小威廉·K·博斯 | Repair and rejuvenation of tissues using platelet-rich plasma |
| US20180094244A1 (en) * | 2015-02-18 | 2018-04-05 | Enlivex Therapeutics Ltd. | Combination immune therapy and cytokine control therapy for cancer treatment |
| CN105567630A (en) * | 2016-01-14 | 2016-05-11 | 齐湘杰 | Kit for synchronously separating cord blood PRP, cord blood plasma and cord blood cells |
| CN107281467A (en) * | 2017-05-22 | 2017-10-24 | 胡建红 | A kind of preparation method of cell factor for autogenous cell skin regeneration |
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Application publication date: 20190111 |
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