CN109303759A - A kind of preparation method and applications of Cord blood platelet rich plasma - Google Patents

A kind of preparation method and applications of Cord blood platelet rich plasma Download PDF

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Publication number
CN109303759A
CN109303759A CN201811413104.XA CN201811413104A CN109303759A CN 109303759 A CN109303759 A CN 109303759A CN 201811413104 A CN201811413104 A CN 201811413104A CN 109303759 A CN109303759 A CN 109303759A
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layer
blood
platelet
rich plasma
plasma
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张权
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Beijing Anyi Biotechnology Co Ltd
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Beijing Anyi Biotechnology Co Ltd
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/98—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin
    • A61K8/981—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin of mammals or bird
    • A61K8/983—Blood, e.g. plasma
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A61Q19/08—Anti-ageing preparations
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
    • A61K2800/805—Corresponding aspects not provided for by any of codes A61K2800/81 - A61K2800/95

Abstract

The present invention relates to cell factor cosmetic fields, in particular to a kind of preparation method and applications of Cord blood platelet rich plasma.A kind of preparation method of Cord blood platelet rich plasma, comprising the following steps: Cord blood 180-200g centrifugation, obtained whole blood are divided into 3 layers, and upper layer is faint yellow plasma layer, and lowest level is red blood cell layer, there is one layer of white yellow clothes layer among the two；The red blood cell near upper layer, white yellow clothes layer and white yellow clothes layer is taken, 800 ± 50g centrifugation, bottom of the tube white precipitate is Platelet Concentrate, siphons away upper plasma, and remaining liq mixes well, as platelet rich plasma.This method has the function of significantly being enriched with blood platelet, and obtained platelet rich plasma mainly contains blood platelet, TGF-β 1, PDGF, VEGF etc., is improved using rear skin hydration degree, and transepidermal water loss is reduced, and elasticity is remarkably reinforced, and improves to skin condition obvious.

Description

A kind of preparation method and applications of Cord blood platelet rich plasma
Technical field
The present invention relates to Cord blood platelet rich plasma cosmetic fields, small in particular to a kind of Cord blood richness blood The preparation method and applications of plate blood plasma.
Background technique
Platelet rich plasma (PRP) is the platelet concentrate rich in the raised growth factor obtained by centrifugal blood, Each blood platelet contains nearly 50-80 α-particle, though α-particle diameter only 200-500nm, includes that nearly 300 kinds of biologies are living Property albumen, these ingredients increase the growth factor of tissue damage part, are controlled to reach by growth factor the reparation of tissue The purpose for the treatment of, therefore play an important role in tissue damage reparation.
A large amount of bioactive molecules and a small amount of leucocyte and red blood cell can be discharged after platelet activation, only pass through de- Grain can just make growth factor (such as platelet derived growth factor (PDGF), transforminggrowthfactor-β1, β 2 (TGF-β 1, β 2), at fibre It ties up Porcine HGF (FGF), vascular endothelial growth factor (VEGF), insulin-like growth factor (IGF) and epidermal growth Factor (EGF) etc.) largely release.Platelet derived growth factor (PDGF) can stimulate fibroblastic generation, chemotactic, thorn Swash transforminggrowthfactor-β1, synthesis collagen etc..These growth factors can help skin delaying senility, improve collagen content and tissue Interior new vessels content makes skin that vigor and gloss be presented.
In view of this, the present invention is specifically proposed.
Summary of the invention
The first object of the present invention is to provide a kind of preparation method of Cord blood platelet rich plasma, and this method has bright The effect of aobvious enrichment blood platelet, obtained platelet rich plasma mainly contain blood platelet, TGF-β 1, PDGF, VEGF etc..
The second object of the present invention is that providing platelet rich plasma is improving the application in skin condition, uses rear skin Hydrauture improves, and transepidermal water loss is reduced, and elasticity is remarkably reinforced.
In order to realize above-mentioned purpose of the invention, the following technical scheme is adopted:
A kind of preparation method of Cord blood platelet rich plasma, comprising the following steps: Cord blood 180-200g centrifugation obtains Whole blood be divided into 3 layers, upper layer is faint yellow plasma layer, and lowest level is red blood cell layer, there is one layer of white yellow clothes layer among the two；
Take the red blood cell near upper layer, white yellow clothes layer and white yellow clothes layer, 800 ± 50g centrifugation, bottom of the tube white precipitate As Platelet Concentrate siphons away upper plasma, and remaining liq mixes well, as platelet rich plasma.
The preparation method of Cord blood platelet rich plasma provided by the invention has the function of significantly being enriched with blood platelet, Obtained platelet rich plasma mainly contains blood platelet, TGF-β 1, PDGF, VEGF etc..
Further, the time of the 180-200g centrifugation is 8-15min.It, can be with as in various embodiments are as follows: It is what 10min, 200g were centrifuged that the time of 180g centrifugation, which is the time that the time that 15min, 190g are centrifuged is 10min, 200g centrifugation, It is 10min etc. that time, which is the time of 8min, 180g centrifugation,.
Further, the time of 800 ± 50g centrifugation is 8-15min.It, can be with as in various embodiments are as follows: 800g centrifugation time be 10min, 800g centrifugation time be 8min, 800g centrifugation time be 15min, 750g centrifugation when Between be 15min, 850g centrifugation time be 8min etc..
In the present invention, is agglomerated in order to prevent after Cord blood separation, need that anti-coagulants is added.Anti-coagulants selects existing reagent And content addition.
The Cord blood used in the present invention is the Cord blood containing anticoagulant citrate dextrose solution A.
Skin is stacked by the fine and close multilayer tissue of construction with cell, is broadly divided into epidermis, corium and subcutaneous tissue.Skin The aging of skin is that physiologic factor and external factor are coefficient as a result, physiologic factor such as age, endocrine and immune function etc., External factor such as ultraviolet light, wind etc..The main reason for aging of skin corium is skin aging, skin corium is directly and containing transparent The matrix of many kinds of substance such as matter acid, compound protein sugar is connected, these matrix are the metabolic conversions such as various water-soluble substances, electrolyte Place, have most direct relationship with skin aging.The aging for delaying skin corium matrix delays the reduction of matrix capilary amount, Aging course can be effectively relieved.The prolonged and repeated irradiation of solar UV is the influence most important factor of skin aging in environment, It can result in pachylosis, wrinkle intensification, pigmentation, blood vessel dilatation, the denaturation of corium elastomer etc..
In the development course of skin care item, the first generation is the grease type cosmetics of simple physical protection, such as sheep oil, second Dai Ze is the aliphatic compound of synthesis, such as odium stearate, and the main component of the third generation is the extract of natural plants.4th For biotechnology skin care item, is extracted using biotechnology and manufacture biological fine similar with human body self structure and with high-affinity Magnificent substance, platelet rich plasma are exactly one type, and skin nursing can be turned to cellular water by the various factors that stem cell is rich in It is flat, fundamentally realize the rejuvenation of skin condition.
Platelet rich plasma provided by the invention mainly contains blood platelet, TGF-β 1, PDGF, VEGF etc..
1) platelet derived growth factor (PDGF)
PDGF is a kind of factor,mitogenic, can stimulate cell mass division growth, promotes the generation of fibroblast, To keep wrinkle naturally long flat.Fibroblastic generation, chemotactic, stimulation transforminggrowthfactor-β1, rubber polymer can also be stimulated Original 1 promotes veins beneath the skin to be formed, and repairs subcutaneous blood microcirculqtory system, provides sufficient nutrition for skin, more effectively delay skin Skin aging.
2) transforminggrowthfactor-β1 (TGF-β 1)
TGF-β 1 is fibroblastic major chemokine, can stimulate fibroblast division growth.TGF-β 1 is right Fibroblastic inducing chemotactic is conducive to the progress of process of tissue reparation.TGF-β 1 can also inducing macrophage secretion blood vessel Occurrence factor accelerates vascularization, can also promote the synthesis of fibronectin, hyaluronic acid etc., therefore skin can obtain It is repaired to good, becomes smooth ruddy.
3) vascular endothelial growth factor (VEGF)
VEGF is that presently found effect is most strong, the highest angiogenic factors of specificity.Skin epidermis tissue is without blood Pipe structure, it is main to provide nutriment by dermal microvascular and metabolic waste object is discharged.The blood vessel number of old application on human skin is sharply It reduces, it is insufficient so as to cause the nutrition supply of skin, hinder the metabolism of cell.It is logical that VEGF can effectively improve local vascular Permeability provides sufficient nutriment and growth factor for fibroblastic proliferation and collage synthesis, promotes cell division, change Kind skin microcirculation, is that skin metabolism product is easy to be discharged, it is not easy to form dark sore, blackspot and chloasma etc..
Platelet rich plasma made from the above method provided by the invention is improving the application in skin condition.
Each component coordinated is improved using rear skin hydration degree, and transepidermal water loss is reduced, and elasticity is remarkably reinforced, and improves skin Skin state effect is obvious.
The present invention also provides a kind of biological agents, contain platelet rich plasma made from above-mentioned preparation method.
The biological agent of different dosage forms or packaging can be made in platelet rich plasma provided by the invention according to use demand.
Further, the platelet rich plasma is stored in fibrin ferment-calcium chloride activator；
The fibrin ferment-calcium chloride activator is the mixed liquor of fibrin ferment and calcium chloride, wherein the calcium chloride is with chlorination The form of calcium solution is added, and the mass percent concentration of the calcium chloride solution is 10% ± 2%, with the volume of calcium chloride solution Meter, the additive amount of the fibrin ferment are 1000 ± 200U/mL.
Further, the fibrin ferment is thrombin of beef.
Further, the platelet rich plasma and the fibrin ferment-calcium chloride activator are mixed for 8-15:1 by volume It is even.
Further, after the mixing, 25-30s, the platelet rich plasma and fibrin ferment-calcium chloride activator are stood Mixed liquor gelled.
The step of obtained spawn can be dispensed and be saved such as is placed on 4 DEG C of preservations.
Spawn obtained can be directly used for using.
It is specifically used that steps are as follows:
1) face cleaning facial cleanser wash clean, naturally dry.
2) dedicated one layer of anaesthetic of face, after smearing uniformly is smeared, one layer of covering preservative film leads to thickness to prevent anaesthetic flowing Unevenly, expose the positions such as eye, nose, mouth, wait 60-70min.
3) clear water cleans anaesthetic, and operator puts on sterile gloves, opens sterile gauze, dips appropriate skin degerming with newly Geramine, wiping face twice, carry out facial disinfection.
4) remaining bromogeramine is wiped out with dry sterile gauze, until facial no moisture residual.
5) adjusting electronic micropin length is 1mm, carries out micropin needle thorn in Face and cheek precedence sequence, guarantees that every piece of skin all relates to And there is micro bleeding category normal phenomenon.
6) spawn being ready for is smeared simultaneously, is smeared uniformly with hand, is slowly absorbed platelet rich plasma.
7) the several sensitive parts of eyelid, forehead, the bridge of the nose, upper lip suitably reduce micropin length to 0.7-0.9mm.
8) order needle is pierced to covering full face, while smearing spawn, is constantly applied to absorption.
9) it is coated with the moisture saver mask of 4 DEG C of cryo-conservations in advance 20 minutes, makes skin calm.
With traditional skin care condition ratio, platelet rich plasma can activate Skin Cell from inside to outside, improve skin from skin corium Function, by this and face, improvement can more persistently.Platelet rich plasma effectively can be sent to epidermis hereinafter, more by micropin Be conducive to the absorption of the factor, skin regeneration can be excited from inside to outside, thus can more enduringly improve skin condition.
The present invention also provides a kind of cosmetics, contain platelet rich plasma made from above-mentioned preparation method or above-mentioned Biological agent.
Further, the cosmetics include toner, lotion, face cream, eye cream.
Compared with prior art, the invention has the benefit that
(1) preparation method of platelet rich plasma provided by the invention, method is easy, and obtained cell factor type is rich Richness, content are high.
(2) biological agent made of platelet rich plasma provided by the invention is improved using rear skin hydration degree, percutaneous to lose Water is reduced, and elasticity is remarkably reinforced, obvious to skin condition improvement.
(3) the present invention also provides the cosmetics containing platelet rich plasma, provide bigger development sky for the nursing of skin Between.
Detailed description of the invention
In order to more clearly explain the embodiment of the invention or the technical proposal in the existing technology, to embodiment or will show below There is attached drawing needed in technical description to be briefly described.
Fig. 1 is that state change compares photo in experimental example 1 of the present invention；
Fig. 2 is another group of comparison photo of state change in experimental example 1 of the present invention；
Fig. 3 is another group of comparison photo of state change in experimental example 1 of the present invention.
Specific embodiment
Embodiment of the present invention is described in detail below in conjunction with embodiment, but those skilled in the art will Understand, the following example is merely to illustrate the present invention, and is not construed as limiting the scope of the invention.It is not specified in embodiment specific Condition person carries out according to conventional conditions or manufacturer's recommended conditions.Reagents or instruments used without specified manufacturer is The conventional products that can be obtained by commercially available purchase.
Embodiment 1
Biological agent containing Cord blood platelet rich plasma, preparation step are as follows:
1) the 15mL Cord blood containing anticoagulant citrate dextrose solution A is placed in 15mL centrifuge tube, 200 × g centrifugation 10min。
2) whole blood is divided into 3 layers, and bottom 1/2 layer is red blood cell layer, and upper layer is faint yellow plasma layer, has one layer among the two Thin white yellow clothes layer.
3) red blood cell near whole supernatants and yellow clothes layer and yellow clothes layer is taken, 800 × g is centrifuged 10min, and centrifugation bottom of the tube is white Color precipitating is Platelet Concentrate, siphons away 3/4 blood plasma of upper layer, remaining liq mixes well, as platelet rich plasma (PRP).
4) under aseptic condition, 10% calcium chloride solution of 1mL is taken, is dissolved with 1000U thrombin of beef, is mixed, as blood coagulation Enzyme-calcium chloride activator, is configured using preceding 1h, 4 DEG C of preservations.
5) PRP and fibrin ferment-calcium chloride activator prepared is mixed by 10:1, stands 30s, gelled.
Through detecting, platelet count is (223.99 ± 44.70) × 10 in Cord blood9/ L, gel manufactured in the present embodiment Platelet count is (1446.60 ± 353.95) × 10 in shape PRP96.45 times of/L, about Whole blood platelet counting, have bright Aobvious enrichment.
The component in gel PRP is detected, it is as a result as follows: 1 concentration 221.34 ± 19.78ng/mL, PDGF concentration of TGF-β For 125.32 ± 33.5ng/mL, VEGF concentration is 23.85 ± 4.2ng/mL.
Embodiment 2
Biological agent containing Cord blood platelet rich plasma, preparation step are as follows:
1) the 15mL Cord blood containing anticoagulant citrate dextrose solution A is placed in 15mL centrifuge tube, 180 × g centrifugation 15min。
2) whole blood is divided into 3 layers, and bottom 1/2 layer is red blood cell layer, and upper layer is faint yellow plasma layer, has one layer among the two Thin white yellow clothes layer.
3) red blood cell near whole supernatants and yellow clothes layer and yellow clothes layer is taken, 850 × g is centrifuged 8min, and centrifugation bottom of the tube is white Color precipitating is Platelet Concentrate, siphons away 3/4 blood plasma of upper layer, remaining liq mixes well, as platelet rich plasma.
4) under aseptic condition, 1mL10% calcium chloride solution is taken, is dissolved with 1000U thrombin of beef, is mixed, as fibrin ferment- Calcium chloride activator is configured using preceding 1h, 4 DEG C of preservations.
5) PRP and fibrin ferment-calcium chloride activator prepared is mixed by 10:1, stands 30s, gelled.
Through detecting, blood platelet is about 6.4 times or more that Whole blood platelet counts in gel PRP manufactured in the present embodiment, With apparent enrichment.
It detects the component in gel PRP, constituent species and content and embodiment 1 is almost the same.
Embodiment 3
Biological agent containing Cord blood platelet rich plasma, preparation step are as follows:
1) the 15mL Cord blood containing anticoagulant citrate dextrose solution A is placed in 15mL centrifuge tube, 200 × g centrifugation 10min。
2) whole blood is divided into 3 layers, and bottom 1/2 layer is red blood cell layer, and upper layer is faint yellow plasma layer, has one layer among the two Thin white yellow clothes layer.
3) red blood cell near whole supernatants and yellow clothes layer and yellow clothes layer is taken, 750 × g is centrifuged 15min, and centrifugation bottom of the tube is white Color precipitating is Platelet Concentrate, siphons away 3/4 blood plasma of upper layer, remaining liq mixes well, as platelet rich plasma.
4) under aseptic condition, 1mL10% calcium chloride solution is taken, is dissolved with 1000U thrombin of beef, is mixed, as fibrin ferment- Calcium chloride activator is configured using preceding 1h, 4 DEG C of preservations.
5) PRP and fibrin ferment-calcium chloride activator prepared is mixed by 10:1, stands 30s, gelled.
Through detecting, blood platelet is about 6.4 times or more that Whole blood platelet counts in gel PRP manufactured in the present embodiment, With apparent enrichment.
It detects the component in gel PRP, constituent species and content and embodiment 1 is almost the same.
Comparative example 1
Application No. is a kind of platelet growth factor preparation method for beautifying skin disclosed in 201410538067.0, Platelet growth factor is obtained with the blood acquired from human body, platelet growth factor includes growth factor PDGF, TGF-β, Including following preparation step:
1) blood of human body is placed in blood sampling test tube, with rpm:2000r/min revolving speed, RCF:2000g centrifugation in centrifuge Power rotates 5 minutes, isolates blood plasma, blood platelet, blood cell；
2) blood sampling test tube separation storage is respectively adopted in gained blood plasma, blood platelet；
3) the dedicated calcium chloride of medical treatment is separately added into blood plasma, blood platelet blood sampling test tube, as growth factor in blood platelet Or in blood plasma collagen activator, the dedicated calcium chloride merging amount of medical treatment is the 10% of blood plasma or blood platelet weight, both is made The pH value for maintaining 7.0 and the 2 hours time of co-incubation at a temperature of 25 DEG C；
4) by, with rpm:8000r/min revolving speed, RCF:8000g is centrifuged in blood plasma, blood platelet blood sampling test tube merging centrifuge Power rotates 5 minutes, does secondary separation；
5) blood plasma, blood platelet blood sampling test tube are taken out, is no more than 5 hours in 25 DEG C of at a temperature of co-incubation.
Comparative example 2
The each component of gel PRP in embodiment 1 is configured by commercial product, wherein platelet content 1450 × 109/ L, 1 concentration 230ng/mL, PDGF concentration of TGF-β are 130ng/mL, and VEGF concentration is 25ng/mL.
Experimental example 1
The present invention also provides biological agents made from above-mentioned preparation method to improve the application in skin condition.
It is specifically used that steps are as follows:
1) face cleaning facial cleanser wash clean, naturally dry.
2) dedicated one layer of anaesthetic of face, after smearing uniformly is smeared, one layer of covering preservative film leads to thickness to prevent anaesthetic flowing Unevenly, expose the positions such as eye, nose, mouth, wait 60-70min.
3) clear water cleans anaesthetic, and operator puts on sterile gloves, opens sterile gauze, dips appropriate skin degerming with newly Geramine, wiping face twice, carry out facial disinfection.
4) remaining bromogeramine is wiped out with dry sterile gauze, until facial no moisture residual.
5) adjusting electronic micropin length is 1mm, carries out micropin needle thorn in Face and cheek precedence sequence, guarantees that every piece of skin all relates to And there is micro bleeding category normal phenomenon.
6) the rich platelet gel being ready for is smeared simultaneously, is smeared uniformly with hand, is slowly absorbed compound.
7) the several sensitive parts of eyelid, forehead, the bridge of the nose, upper lip suitably reduce micropin length to 0.7-0.9mm.
8) order needle is pierced to full face is covered, and is smeared rich platelet gel, is constantly applied to absorption.
9) it is coated with the moisture saver mask of 4 DEG C of cryo-conservations in advance 20 minutes, makes skin calm.
Experimental group: sharing 13 people, male or female, and the age 33-60 years old.The rich platelet provided using the embodiment of the present invention 1 After gel for treating 5 times, effect comparison is carried out, partial results are as shown in table 1.
1 therapeutic effect of table compares
Whole testing numbers are counted it has been found that after rich platelet gel for treating 5 times provided using the embodiment of the present invention 1, water Right promotion reaches as high as 26%, assembly average is 15% or so 9.5% or more；Transepidermal water loss reduce 7% with On, 27% is reached as high as, assembly average is 14% or so；Elasticity is promoted 4.5% or more, reaches as high as 10%, statistics Average value is 7.5% or so.
For certain embodiments picture results of comparison as shown in Figure 1-3, wherein, A is picture before treating, and B is picture after treatment.
1 group of comparative example: share 5 people, male or female, the age 33-60 years old.Liquid undergoing treatment 5 times provided using comparative example 1 Afterwards, effect comparison is carried out, the results are shown in Table 2.
2 therapeutic effect of table compares
After liquid undergoing treatment 5 times of comparative example 1 of the present invention offer, hydrauture promotes the assembly average within 6% 3% or so；Transepidermal water loss reduces within 15%, and assembly average is 10% or so；Elasticity is promoted, and assembly average exists 5% or so.
2 groups of comparative example: share 5 people, male or female, the age 33-60 years old.Liquid undergoing treatment 5 times provided using comparative example 2 Afterwards, effect comparison is carried out, the results are shown in Table 3.
3 therapeutic effect of table compares
After liquid undergoing treatment 5 times of comparative example 2 of the present invention offer, hydrauture promotes the statistical average within 5.1% Value is 3% or so；Transepidermal water loss reduces within 12.2%, and assembly average is 10% or so；Elasticity is promoted, statistical average Value is 4% or so.
It is above-mentioned it is found that Cord blood platelet rich plasma gel provided by the invention is obviously improved using rear skin hydration degree, Transepidermal water loss is reduced, and elasticity is remarkably reinforced, and has obvious effect to skin condition is improved.
Although illustrate and describing the present invention with specific embodiment, it will be appreciated that without departing substantially from of the invention Many other change and modification can be made in the case where spirit and scope.It is, therefore, intended that in the following claims Including belonging to all such changes and modifications in the scope of the invention.

Claims (10)

1. a kind of preparation method of Cord blood platelet rich plasma, which comprises the following steps: Cord blood 180-200g Centrifugation, obtained whole blood are divided into 3 layers, and upper layer is faint yellow plasma layer, and lowest level is red blood cell layer, there is one layer of white among the two Yellow clothes layer；
The red blood cell near upper layer, white yellow clothes layer and white yellow clothes layer, 800 ± 50g centrifugation are taken, bottom of the tube white precipitate is Platelet Concentrate siphons away upper plasma, and remaining liq mixes well, as platelet rich plasma.
2. preparation method according to claim 1, which is characterized in that the time of the 180-200g centrifugation is 8-15min.
3. preparation method according to claim 1, which is characterized in that the time of 800 ± 50g centrifugation is 8-15min.
4. preparation method according to claim 1-3, which is characterized in that the Cord blood is to contain citric acid Portugal The Cord blood of grape sugar anticoagulant solution A.
5. platelet rich plasma made from the described in any item preparation methods of claim 1-4 is improving answering in skin condition With.
6. a kind of biological agent, which is characterized in that small containing richness blood made from the described in any item preparation methods of claim 1-4 Plate blood plasma.
7. biological agent according to claim 6, which is characterized in that the platelet rich plasma is stored in fibrin ferment-chlorine Change in calcium activator；
The fibrin ferment-calcium chloride activator is the mixed liquor of fibrin ferment and calcium chloride, wherein the calcium chloride is molten with calcium chloride The form of liquid is added, and the mass percent concentration of the calcium chloride solution is 10% ± 2%, in terms of the volume of calcium chloride solution, The additive amount of the fibrin ferment is 1000 ± 200U/mL；
Further, the fibrin ferment is thrombin of beef.
8. biological agent according to claim 7, which is characterized in that the platelet rich plasma and the fibrin ferment-chlorine Change calcium activator is that 8-15:1 is mixed by volume；
Further, after the mixing, 25-30s is stood, the platelet rich plasma and fibrin ferment-calcium chloride activator are mixed Close liquid gelled.
9. a kind of cosmetics, which is characterized in that contain rich platelet made from the described in any item preparation methods of claim 1-4 Blood plasma or the described in any item biological agents of claim 6-8.
10. cosmetics according to claim 9, which is characterized in that the cosmetics include lotion, face cream, eye cream.
CN201811413104.XA 2018-11-23 2018-11-23 A kind of preparation method and applications of Cord blood platelet rich plasma Pending CN109303759A (en)

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Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112553155A (en) * 2020-12-28 2021-03-26 山东省齐鲁干细胞工程有限公司 Umbilical cord blood mesenchymal stem cell culture method
CN112831467A (en) * 2021-01-20 2021-05-25 四川省肿瘤医院 Blood platelet extraction method
CN113046317A (en) * 2021-03-31 2021-06-29 河南省遗传资源细胞库有限公司 Method for extracting platelet-rich plasma based on preparation of umbilical cord blood hematopoietic stem cells
CN113750031A (en) * 2021-10-12 2021-12-07 深圳市泓浩生物科技有限公司 Antioxidant composition, preparation method thereof and skin care product
CN114225017A (en) * 2021-12-30 2022-03-25 武汉冉谷医疗有限公司 Composition for treating osteoarthritis or repairing cartilage damage and preparation method thereof
CN116407559A (en) * 2023-05-25 2023-07-11 云南康旭生物科技有限公司 Preparation for treating premature ovarian failure and preparation method thereof

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102367435A (en) * 2011-11-04 2012-03-07 四川新生命干细胞科技股份有限公司 Preparation of human platelet-rich plasma and application of same in isolation and culture of human mesenchymal stem cells
CN105567630A (en) * 2016-01-14 2016-05-11 齐湘杰 Kit for synchronously separating cord blood PRP, cord blood plasma and cord blood cells
CN106236779A (en) * 2016-08-22 2016-12-21 孔五 A kind of preparation method of Cord blood platelet rich plasma PRP
CN106754681A (en) * 2016-12-29 2017-05-31 李众利 A kind of platelet rich plasma and preparation method and application
CN107496324A (en) * 2017-08-10 2017-12-22 河南省银丰生物工程技术有限公司 A kind of anti-aging face filler based on umbilical cord mesenchymal stem cells and preparation method thereof
CN107921184A (en) * 2015-06-04 2018-04-17 冯达泽尼艾瑞斯卡格兰达医院马乔里门诊部 More bags of system and the method for being used to prepare blood constituent

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102367435A (en) * 2011-11-04 2012-03-07 四川新生命干细胞科技股份有限公司 Preparation of human platelet-rich plasma and application of same in isolation and culture of human mesenchymal stem cells
CN107921184A (en) * 2015-06-04 2018-04-17 冯达泽尼艾瑞斯卡格兰达医院马乔里门诊部 More bags of system and the method for being used to prepare blood constituent
CN105567630A (en) * 2016-01-14 2016-05-11 齐湘杰 Kit for synchronously separating cord blood PRP, cord blood plasma and cord blood cells
CN106236779A (en) * 2016-08-22 2016-12-21 孔五 A kind of preparation method of Cord blood platelet rich plasma PRP
CN106754681A (en) * 2016-12-29 2017-05-31 李众利 A kind of platelet rich plasma and preparation method and application
CN107496324A (en) * 2017-08-10 2017-12-22 河南省银丰生物工程技术有限公司 A kind of anti-aging face filler based on umbilical cord mesenchymal stem cells and preparation method thereof

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
余晶 等: "微针导入自体富血小板血浆对面部年轻化的疗效研究", 《中国美容医学》 *
张远 等: "小型猪富血小板血浆对牙周膜干细胞的成骨诱导", 《中国组织工程研究》 *
马良彧 等: "富血小板血浆治疗椎间盘退变：从基础研究到临床应用", 《中国组织工程研究》 *

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112553155A (en) * 2020-12-28 2021-03-26 山东省齐鲁干细胞工程有限公司 Umbilical cord blood mesenchymal stem cell culture method
CN112831467A (en) * 2021-01-20 2021-05-25 四川省肿瘤医院 Blood platelet extraction method
CN113046317A (en) * 2021-03-31 2021-06-29 河南省遗传资源细胞库有限公司 Method for extracting platelet-rich plasma based on preparation of umbilical cord blood hematopoietic stem cells
CN113750031A (en) * 2021-10-12 2021-12-07 深圳市泓浩生物科技有限公司 Antioxidant composition, preparation method thereof and skin care product
CN113750031B (en) * 2021-10-12 2024-04-05 深圳市泓浩生物科技有限公司 Antioxidant composition and preparation method thereof
CN114225017A (en) * 2021-12-30 2022-03-25 武汉冉谷医疗有限公司 Composition for treating osteoarthritis or repairing cartilage damage and preparation method thereof
CN116407559A (en) * 2023-05-25 2023-07-11 云南康旭生物科技有限公司 Preparation for treating premature ovarian failure and preparation method thereof

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