CN103751768A - Preparation helping to heal wound - Google Patents
Preparation helping to heal wound Download PDFInfo
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- CN103751768A CN103751768A CN201310629784.XA CN201310629784A CN103751768A CN 103751768 A CN103751768 A CN 103751768A CN 201310629784 A CN201310629784 A CN 201310629784A CN 103751768 A CN103751768 A CN 103751768A
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Abstract
A disclosed preparation helping to heal wound comprises basic fibroblast growth factor (bFGF), vascular endothelial growth factor (VEGF), transforming growth factor (TGFbeta), granulocyte-macrophage colony-stimulating factor (GM-CSF), hepatocyte growth factor (HGF), platelet-derived growth factor (PDGF), interleukin (IL2) and interleukin 10 (IL10). The preparation is a composition rich in abundant nutritional factors, and is capable of helping to accelerate wound healing without scar and pigementation.
Description
Technical field
The present invention relates to biological medicine, particularly wound healing.
Background technology
Skin trauma is the injury that people often encounter in daily life, and skin healing process is slow, also deals with improperly and can infect, fester and prolongedly not heal.The degree of depth skin that a variety of causes causes is dampened, scratch, and the wound of surgical operation, gynecilogical operation, plastic aesthetic surgery all easily forms cicatrix in agglutination, to people, brings great worry and worry.How in accelerating wound, to make skin healing smooth, be that medical research personnel attempt the difficult problem solving always.
Summary of the invention
The object of this invention is to provide a kind of compositions that abundant trophic factors helps accelerating wound healing that is rich in.
In some embodiments, the preparation that helps wound healing, comprises basic fibroblast growth factor (bFGF), VEGF (VEGF), transforming growth factor (TGF β), the granular leukocyte colony stimulating growth factor (GM-CSF), hepatocyte growth factor (HGF), platelet derived growth factor (PDGF), interleukin-22 (TL2) and IL-10 (IL10).
Wherein, the biological action of bFGF is very strong, is important factor,mitogenic, is also the inducible factor of newborn Skin Cell form neurogenesis and development, can promote wound healing and tissue repair, promote tissue regeneration.In the process of skin regeneration, bFGF can improve the microenvironment of Growth of Cells, promotes the synthetic of elastic fiber and collagen protein, makes skin high resilience, makes skin in sliding tender state.BFGF can also reduce the content of melanin and coloured corpuscle in Skin Cell, alleviates the calmness of skin pigment.BFGF has the formation of very strong chafe granulation tissue and promotes the epithelization of granulation tissue, regulates collagen degradation and renewal, thus the effect of shortening the wound healing time and reducing cicatrization.
VEGF is the most effective angiogenic growth factor, that the short endothelial cell mitogen of a kind of selectivity is former, it can increase the penetrating ability of blood vessel, promote endothelial cell proliferation, and the development of the combination quantity regulating blood vessel by receptor, promote blood vessel and vasculolymphatic generation, for skin trauma repair process provides enough oxygen and nutrient substance, be considered to the basis of tissue regeneration.
TGF-β is a kind of polypeptide growth factor, and its biological function is propagation, the differentiation that affects cell, also at extracellular matrix, forms, organizes the important roles such as formation and reconstruction.TGF-β also plays an important role in the immunologic function that strengthens cell especially.
GM-CSF is not only a kind of hemopoietic growth factor of pluripotency, and can effectively promote wound healing.Because wound healing is a process that comprises immunity and angiogenesis, GM-CSF acts on hemopoietic progenitor cell, promotes its propagation, differentiation, and stimulates it to peripheral blood, to discharge, and can maintain the survival of hemopoietic progenitor cell.In recent years research shows, endothelial progenitor cells participation angiogenesis.Therefore, GM-CSF, by promoting propagation, differentiation and the migration of endothelial progenitor cells, promotes generation and the growth of newborn Skin Cell and tissue.
HGF is that the DNA of cell cultured supernatant is synthetic, and a kind of albumen activity factor plays an important role in liver regeneration.More and more many reports show in recent years, and HGF does not just act on liver regeneration, and to the growth of many tissues and cell, broken up important regulating and controlling effect.HGF has powerful promotion endothelial cell proliferation effect, and effect is obviously better than bFGF and VEGF.HGF also can be by stimulating the propagation of vascular endothelial cell to promote the angiogenesis of wounded tissue.
PDGF is a stimulant mitogen that mainly contains for various kinds of cell, can stimulate especially contiguous connective tissue cell growth.These connective tissue cells are the vanguards that rebuild damaged tissues, healing wound.Experiment shows that PDGF plays an important role in the overall process of wound healing, strengthens the formation of granulation tissue, promotes wound healing and shortens healing time.PDGF can also promote the synthetic of collagen protein, makes newborn skin have good elasticity.
TL2 and TL10 are two important members of interleukin factor family, they all play a significant role in a series of processes such as maturation, activation, propagation and immunomodulating of immunocyte, in addition they also participate in multiple physiology and the pathological reaction of body, in repair in trauma, play an important role.The preparation of help wound healing of the present invention comprises above-mentioned somatomedin and helps accelerating wound healing and do not stay cicatrix and pigmentation.
The present invention has been obtained a kind of fast wound healing and has not been stayed cicatrix and Pigmented compositions by inventor scientific research personnel's the above-mentioned somatomedin of combination.
In some embodiments, helping preparation bFGF (bFGF) concentration of wound healing is that 50ng/ml, VEGF (VEGF) concentration are that 20pg, transforming growth factor (TGF β) 350ng/ml, the granular leukocyte colony stimulating growth factor (GM-CSF) concentration are that 65ng/ml, hepatocyte growth factor (HGF) concentration are that 0.65ng/ml, platelet derived growth factor concentration are that (PDGF) 15pg/ml, interleukin-22 (TL2) concentration are that 40pg/ml and IL-10 (TL10) concentration are 45pg/ml.
Because above-mentioned various somatomedin have very strong biological activity, the present invention is used in combination these components and only need uses very low concentration just can reach required effect.
Accompanying drawing explanation
Tissue slice figure after Fig. 1 effect experiment of the present invention normal saline matched group skin wound healing.
Tissue slice figure after the wound healing of Fig. 2 effect experiments experiment of the present invention group.
The specific embodiment
Below in conjunction with accompanying drawing, invention is described in further detail.
Embodiment 1 helps the preparation preparation of wound healing
Make following solution 1L with normal saline preparation filtration sterilization, obtain solution A.
Helping preparation bFGF (bFGF) concentration of wound healing is 50ng/ml, VEGF (VEGF) concentration is 20pg, transforming growth factor (TGF β) 350ng/ml, the granular leukocyte colony stimulating growth factor (GM-CSF) concentration is 65ng/ml, hepatocyte growth factor (HGF) concentration is 0.65ng/ml, platelet derived growth factor concentration is (PDGF) 15pg/ml, interleukin-22 (TL2) concentration is that 40pg/ml and IL-10 (IL10) concentration are 45pg/ml.
1, help the preparation of wound healing in zoopery
Skin trauma model preparation: choose 20 of rats, be divided at random 2 groups (experimental group and blank groups), 10 every group.After anesthetized rat, its extremity are flattened to ventricumbent position and be fixed on plate face, by the make progress region of 8cm of center, back left side 1.5cm, afterbody, amount to the buttocks dorsal area of 3cm * 8cm as field of operation.Art district chaeta will be wiped out as far as possible, pick clean, avoids causing skin injured abnormal simultaneously.At the skin area of getting ready, with gentian voiet, carry out otch labelling.Incision design is at its buttocks back, mark respectively the rectangle wound surface of 1.5cm * 2.0cm size, then sterilize after 3 times repeatedly with ethanol in full back, with knife blade, remove authentic skin, but the deep fascia on muscle injury surface not, removal tissue mass makes just to firstly appear chamber volume and all reaches 1ml, to guarantee that the first dosage of each wound reaches consistent.Afterwards with sthptic sponge filling wound chamber fully hemostasis by compression, afterwards for becoming mould animal.
Treatment administration: make the sterilized cotton ball consistent with skin wound size, each cotton balls can be satisfied and be inhaled the treatment liquid of 1ml, be normal saline (blank group), solution A (A group), making treatment cotton balls is filled in respectively among wound chamber, while noting operation, be sure not to allow treatment liquid be extruded and overflow, affect dose.
Dermal pathology and wound tissue are learned and are observed: in operation, within latter 21 days, sacrifice 20 animals, get wound surface surrounding skin tissue for histopathology research, comprise organization embedding, and microsection manufacture, HE dyes and pathological observation is taken pictures and semi-quantitative assessment.Evaluation index comprises:
(1) inflammatory reaction: leukocyte infiltration.
(2) granulation tissue
(3) collagenous fiber bundle quantity and arrangement (sxemiquantitative)
(4) epidermis cell differentiation degree and layering situation
According to pathological changes light and heavy degree, sxemiquantitative is slight "+" successively, moderate " ++ ", severe " +++ ", or anosis changing.
2, animal skin wound tissue is learned and is observed
Blank group: 3 examples in this group (wherein a routine tissue slice is as Fig. 1) skin all covers crust on visible local epidermis, hemorrhage (+), the degeneration of epidermis pavement epithelium cells moderate, necrosis, erosion (++).Skin corium is shown in a small amount of cell infiltration (+), corium fabric connective tissue proliferation (++), and the appendages of skin obviously reduce (++).4 routine skins are visible epidermis stratified squamous epithelium all, and skin corium is shown in a small amount of cell infiltration (+), corium fabric connective tissue proliferation (++), and the appendages of skin obviously reduce (++).。
Experimental group: this group skin is all without covering crust (wherein a routine tissue slice figure is as Fig. 2) on visible local epidermis.Only 1 routine epidermis squamous cell moderate degeneration (+), skin corium is shown in a small amount of cell infiltration (+), blood capillary proliferation (+), corium fabric connective tissue proliferation (++), the appendages of skin obviously reduce (++); 1 routine skin is visible epidermis stratified squamous epithelium organizational structure all, and skin corium is shown in a small amount of cell infiltration (+), corium fabric connective tissue proliferation (+), and the appendages of skin obviously reduce (+).
3, healing rate is affected
Get healthy adult sD rat, 190～220g, SPF level, male and female half and half, every animal buttocks back cuts out the rectangle wound surface of 1.5cm x2.0cm size with scalpel, remove authentic skin, but the deep fascia on muscle injury surface is not made degree of stressing a skin injury model.Skin injury animal is divided into two groups at random: " a " group is blank group, the cotton balls that dips in normal saline by satisfying is filled among wound chamber." b " group is treatment group, and the embodiment 1 that dips in 1.0mL by satisfying helps the preparation of wound healing to be filled among wound chamber.After 2 days, within every two days, with transparent organic glass, put wound surface top, draw its shape, observe wound surface situation, and by its graph copying to cellophane cover, on image analyzer, accurately measure wound surface area, calculate healing speed.In experiment, MAIN OUTCOME MEASURES is that gross examination of skeletal muscle is respectively organized Rat Wound Healing situation and the average healing and speed.Therapy lasted till the wound of a group laboratory animal heal completely.Result is as table 1.
Table 1 each treated animal skin wound healing time and speed
Conclusion: the preparation that contains basic fibroblast growth factor (bFGF), VEGF (VEGF), transforming growth factor (TGF β), the granular leukocyte colony stimulating growth factor (GM-CSF), hepatocyte growth factor (HGF), platelet derived growth factor (PDGF), interleukin-22 (TL2) and IL-10 (TL10) can effectively help wound surface skin repair.
Above-described is only some embodiments of the present invention.For the person of ordinary skill of the art, without departing from the concept of the premise of the invention, can also make some distortion and improvement, these all belong to protection scope of the present invention.
Claims (2)
1. help the preparation of wound healing, it is characterized in that, comprise basic fibroblast growth factor, VEGF, transforming growth factor, the granular leukocyte colony stimulating growth factor, hepatocyte growth factor, platelet derived growth factor, interleukin-22 and IL-10.
2. the preparation of help wound healing according to claim 1, it is characterized in that, described basic fibroblast growth factor concentration is that 50ng/ml, VEGF concentration are that 20pg/ml, transforming growth factor 350ng/ml, granular leukocyte colony stimulating growth factor concentration are that 65ng/ml, hepatocyte growth factor concentration are that 0.65ng/ml, platelet derived growth factor concentration are that 15pg/ml, interleukin-22 concentration are that 40pg/ml and IL-10 concentration are 45pg/ml.
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| CN201310629784.XA CN103751768A (en) | 2013-11-29 | 2013-11-29 | Preparation helping to heal wound |
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| CN201310629784.XA CN103751768A (en) | 2013-11-29 | 2013-11-29 | Preparation helping to heal wound |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106906180A (en) * | 2016-12-28 | 2017-06-30 | 里程 | A kind of compound additive with bioactivation and its production and use |
| CN108273041A (en) * | 2018-04-11 | 2018-07-13 | 广东颜值科技有限公司 | A kind of preparation and its preparation method and application promoting skin wound healing |
| CN111097066A (en) * | 2020-01-10 | 2020-05-05 | 温州医科大学附属第一医院 | Medical dressing capable of inhibiting scar generation and promoting wound healing and preparation method thereof |
| JP2021506969A (en) * | 2017-12-14 | 2021-02-22 | グリーン・クロス・ウェルビーイング・コーポレイションＧｒｅｅｎ Ｃｒｏｓｓ Ｗｅｌｌｂｅｉｎｇ Ｃｏｒｐｏｒａｔｉｏｎ | Cosmetic composition and pharmaceutical composition for atopic dermatitis, hair loss, wound or skin wrinkle improvement |
Citations (2)
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| CN101503672A (en) * | 2008-02-05 | 2009-08-12 | 湖南惠霖生命科技有限公司 | Composition of cytokine and use thereof |
| CN101940590A (en) * | 2010-08-27 | 2011-01-12 | 上海士腾生物技术有限公司 | Preparation for promoting wound healing and preparation method thereof |
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2013
- 2013-11-29 CN CN201310629784.XA patent/CN103751768A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101503672A (en) * | 2008-02-05 | 2009-08-12 | 湖南惠霖生命科技有限公司 | Composition of cytokine and use thereof |
| CN101940590A (en) * | 2010-08-27 | 2011-01-12 | 上海士腾生物技术有限公司 | Preparation for promoting wound healing and preparation method thereof |
Non-Patent Citations (3)
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| WON-SERK KIM ET AL.: "wound healing effect of adipose-derived stem cells: a critical role of secretory factors on human dermal fibroblasts", 《JOURNAL OF DERMATOLOGICAL SCIENCE》, 31 October 2007 (2007-10-31), pages 15 - 24 * |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106906180A (en) * | 2016-12-28 | 2017-06-30 | 里程 | A kind of compound additive with bioactivation and its production and use |
| JP2021506969A (en) * | 2017-12-14 | 2021-02-22 | グリーン・クロス・ウェルビーイング・コーポレイションＧｒｅｅｎ Ｃｒｏｓｓ Ｗｅｌｌｂｅｉｎｇ Ｃｏｒｐｏｒａｔｉｏｎ | Cosmetic composition and pharmaceutical composition for atopic dermatitis, hair loss, wound or skin wrinkle improvement |
| CN108273041A (en) * | 2018-04-11 | 2018-07-13 | 广东颜值科技有限公司 | A kind of preparation and its preparation method and application promoting skin wound healing |
| CN111097066A (en) * | 2020-01-10 | 2020-05-05 | 温州医科大学附属第一医院 | Medical dressing capable of inhibiting scar generation and promoting wound healing and preparation method thereof |
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Application publication date: 20140430 |