CN109170336A - A kind of feed containing Folium Ginkgo fermented product improving laying hen performance and Egg Quality - Google Patents

A kind of feed containing Folium Ginkgo fermented product improving laying hen performance and Egg Quality Download PDF

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Publication number
CN109170336A
CN109170336A CN201811175446.2A CN201811175446A CN109170336A CN 109170336 A CN109170336 A CN 109170336A CN 201811175446 A CN201811175446 A CN 201811175446A CN 109170336 A CN109170336 A CN 109170336A
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CN
China
Prior art keywords
fermented product
folium ginkgo
ginkgo fermented
laying hen
feed containing
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Pending
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CN201811175446.2A
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Chinese (zh)
Inventor
杨小虎
张旭辉
曹福亮
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Nanjing Forestry University
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Nanjing Forestry University
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Priority to CN201811175446.2A priority Critical patent/CN109170336A/en
Publication of CN109170336A publication Critical patent/CN109170336A/en
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K50/00Feeding-stuffs specially adapted for particular animals
    • A23K50/70Feeding-stuffs specially adapted for particular animals for birds
    • A23K50/75Feeding-stuffs specially adapted for particular animals for birds for poultry
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/10Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/12Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass

Abstract

The present invention relates to chicken feed fields, more particularly to a kind of feed containing Folium Ginkgo fermented product for improving laying hen performance and Egg Quality, the feed is made of Folium Ginkgo fermented product and basal diet, the basal diet is by corn 61.5%, dregs of beans 22%, wheat bran 2%, peanut meal 2%, grease 1%, the composition of mountain flour 8.5% and premix 3%, the Folium Ginkgo fermented product is by ginkgo leaf 42.8%, fermenting microbe 5%, nitrogen source 2.0%, inorganic salts 0.2% and distilled water 50% ferment, wherein Folium Ginkgo fermented product is respectively with the 0.20% of basal diet, 0.35% and 0.50% addition, illustrate that the feed containing Folium Ginkgo fermented product can be improved the laying rate and average egg weight of laying hen, reduce the soft rate of broken eggs and death and culling rate of laying hen, on the daily ingestion amount of laying hen without influence;Feed containing Folium Ginkgo fermented product is significantly increased to eggshell strength and shell thickness, and Folium Ginkgo fermented product has improvement to yolk color, improves the quality of egg.

Description

A kind of feed containing Folium Ginkgo fermented product improving laying hen performance and Egg Quality
Technical field
The present invention relates to chicken feed fields more particularly to a kind of ginkgo leaf that contains for improving laying hen performance and Egg Quality to ferment The feed of object.
Background technique
Laying hen refers to that raising gets up special raw egg to supply the chicken of egg only, and with meat chicken difference, people raise the master of laying hen Wanting project is to improve egg quality and keep or improve egg production, and not improve chicken meat quality.Egg is the master for raising laying hen Want revenue source.Layer breed should select physical strong, and premunition is strong, and power of looking for food is strong, and egg matter is good, and laying rate is high, and thick It raises, adapts to the indigenous chickens of local environment.
In the cultivation of laying hen, for the production performance and Egg Quality for improving laying hen, the feed of chicken is improved.Chinese medicine silver Apricot leaf have more beneficial physiological function, and it is pollution-free, have no toxic side effect, have high nutrition, health care, medical value, To keep up with the trend of the times, by Folium Ginkgo fermented product in conjunction with conventional chicken feed, manufacture a kind of green, nuisanceless and egg can be improved The feed containing Folium Ginkgo fermented product of chicken performance and Egg Quality.
Summary of the invention
The purpose of the present invention is to provide it is a kind of raising laying hen performance and Egg Quality the feed containing Folium Ginkgo fermented product, To solve the above technical problems.
The present invention using following technical scheme in order to solve the above technical problems, realized:
A kind of feed containing Folium Ginkgo fermented product improving laying hen performance and Egg Quality, the feed are fermented by ginkgo leaf Object and basal diet composition, the basal diet by corn 61.5%, dregs of beans 22%, wheat bran 2%, peanut meal 2%, grease 1%, The composition of mountain flour 8.5% and premix 3%, the Folium Ginkgo fermented product is by ginkgo leaf 42.8%, fermenting microbe 5%, nitrogen source 2.0%, inorganic salts 0.2% and distilled water 50% ferment, wherein Folium Ginkgo fermented product respectively with the 0.20% of basal diet, 0.35% and 0.50% addition.
Preferably, the preparation of the Folium Ginkgo fermented product includes the following steps:
S1, prepare raw material: being crushed after ginkgo leaf is air-dried, sieving;
S2, fermenting microbe inoculation: fermenting microbe includes penicillium oxalicum, lactic acid bacteria, bafillus natto, produces Ruan's Candida Bacterium, by four strains, 2:1:2:1 is linked into solid-state fermentation culture medium with 5% inoculum concentration in proportion, penicillium oxalicum × 2: cream Sour bacterium × 1: bafillus natto × 2: producing Ruan saccharomycete × 1, and strain quantity ratio is 2 × 107: 1 × 107: 2 × 107: 1 × 107, It will be inoculated with the bacterium solution that total amount is 5ml on culture medium and carry out mixed fermentation, add cotton plug, triangle bottleneck ox-hide paper bag after being inoculated with Firmly, it is placed in 30 DEG C of constant incubator and cultivates, incubation time 72h;
S3,2.0% nitrogen source is added to triangular flask;
S4,0.2% inorganic salts are added to triangular flask;
S5, fermentation: to triangular flask, adjusting material-water ratio is 1:1, distilled water 25ml, setting for addition ginkgo leaf and distilled water Cultivation temperature is 30 DEG C, and ferment 72h.
Preferably, the nitrogen source is any one of urea, ammonium sulfate, ammonium nitrate and four kinds of ammonium oxalate.
Preferably, the inorganic salts are disodium hydrogen phosphate, sodium dihydrogen phosphate, dipotassium hydrogen phosphate, potassium dihydrogen phosphate, calcium chloride With any one of six kinds of calcium carbonate.
The beneficial effects of the present invention are:
Illustrate that the feed containing Folium Ginkgo fermented product can be improved the laying rate and average egg weight of laying hen, reduces the soft of laying hen Rate of broken eggs and death and culling rate, on the daily ingestion amount of laying hen without influence;Feed containing Folium Ginkgo fermented product is to eggshell strength and eggshell Thickness is significantly increased, and Folium Ginkgo fermented product has improvement to yolk color, improves the quality of egg;
Folium Ginkgo fermented product is a kind of efficient, free of contamination green bio feed addictive, has natural sex and nontoxic pair The characteristics of effect, improves the production performance of laying hen in the production application of laying hen, improves Egg Quality, along with green product Exploitation, there is vast potential for future development, and it as a kind of immunopotentiator, health and disease resistance to livestock and poultry should have very Good facilitation effect.
Specific embodiment
In order to be easy to understand the technical means, the creative features, the aims and the efficiencies achieved by the present invention, tie below Specific embodiment is closed, the present invention is further explained, and however, the following embodiments are merely preferred embodiments of the present invention, and not all. Based on the implementation example in the implementation mode, those skilled in the art's obtained other realities without making creative work Example is applied, protection scope of the present invention is belonged to.Experimental method in following embodiments is unless otherwise specified conventional method, The materials, reagents and the like used in the following examples is commercially available unless otherwise specified.
Embodiment 1
A kind of feed containing Folium Ginkgo fermented product improving laying hen performance and Egg Quality, feed by Folium Ginkgo fermented product and Basal diet composition, basal diet is by corn 61.5%, dregs of beans 22%, wheat bran 2%, peanut meal 2%, grease 1%, mountain flour 8.5% and premix 3% composition, Folium Ginkgo fermented product is by ginkgo leaf 42.8%, fermenting microbe 5%, nitrogen source 2.0%, inorganic Salt 0.2% and distilled water 50% ferment, and wherein Folium Ginkgo fermented product is with 0.20% addition of basal diet.
The preparation of Folium Ginkgo fermented product includes the following steps:
S1, prepare raw material: being crushed after ginkgo leaf is air-dried, sieving;
S2, fermenting microbe inoculation: fermenting microbe includes penicillium oxalicum, lactic acid bacteria, bafillus natto, produces Ruan's Candida Bacterium, by four strains, 2:1:2:1 is linked into solid-state fermentation culture medium with 5% inoculum concentration in proportion, penicillium oxalicum × 2: cream Sour bacterium × 1: bafillus natto × 2: producing Ruan saccharomycete × 1, and strain quantity ratio is 2 × 107: 1 × 107: 2 × 107: 1 × 107, It will be inoculated with the bacterium solution that total amount is 5ml on culture medium and carry out mixed fermentation, add cotton plug, triangle bottleneck ox-hide paper bag after being inoculated with Firmly, it is placed in 30 DEG C of constant incubator and cultivates, incubation time 72h;
S3,2.0% nitrogen source is added to triangular flask;
S4,0.2% inorganic salts are added to triangular flask;
S5, fermentation: to triangular flask, adjusting material-water ratio is 1:1, distilled water 25ml, setting for addition ginkgo leaf and distilled water Cultivation temperature is 30 DEG C, and ferment 72h.
Nitrogen source is any one of urea, ammonium sulfate, ammonium nitrate and four kinds of ammonium oxalate.
Inorganic salts are disodium hydrogen phosphate, sodium dihydrogen phosphate, dipotassium hydrogen phosphate, potassium dihydrogen phosphate, calcium chloride and calcium carbonate six Any one of kind.
Embodiment 2
A kind of feed containing Folium Ginkgo fermented product improving laying hen performance and Egg Quality, feed by Folium Ginkgo fermented product and Basal diet composition, basal diet is by corn 61.5%, dregs of beans 22%, wheat bran 2%, peanut meal 2%, grease 1%, mountain flour 8.5% and premix 3% composition, Folium Ginkgo fermented product is by ginkgo leaf 42.8%, fermenting microbe 5%, nitrogen source 2.0%, inorganic Salt 0.2% and distilled water 50% ferment, and wherein Folium Ginkgo fermented product is with 0.35% addition of basal diet.
The preparation of Folium Ginkgo fermented product includes the following steps:
S1, prepare raw material: being crushed after ginkgo leaf is air-dried, sieving;
S2, fermenting microbe inoculation: fermenting microbe includes penicillium oxalicum, lactic acid bacteria, bafillus natto, produces Ruan's Candida Bacterium, by four strains, 2:1:2:1 is linked into solid-state fermentation culture medium with 5% inoculum concentration in proportion, penicillium oxalicum × 2: cream Sour bacterium × 1: bafillus natto × 2: producing Ruan saccharomycete × 1, and strain quantity ratio is 2 × 107: 1 × 107: 2 × 107: 1 × 107, It will be inoculated with the bacterium solution that total amount is 5ml on culture medium and carry out mixed fermentation, add cotton plug, triangle bottleneck ox-hide paper bag after being inoculated with Firmly, it is placed in 30 DEG C of constant incubator and cultivates, incubation time 72h;
S3,2.0% nitrogen source is added to triangular flask;
S4,0.2% inorganic salts are added to triangular flask;
S5, fermentation: to triangular flask, adjusting material-water ratio is 1:1, distilled water 25ml, setting for addition ginkgo leaf and distilled water Cultivation temperature is 30 DEG C, and ferment 72h.
Nitrogen source is any one of urea, ammonium sulfate, ammonium nitrate and four kinds of ammonium oxalate.
Inorganic salts are disodium hydrogen phosphate, sodium dihydrogen phosphate, dipotassium hydrogen phosphate, potassium dihydrogen phosphate, calcium chloride and calcium carbonate six Any one of kind.
Embodiment 3
A kind of feed containing Folium Ginkgo fermented product improving laying hen performance and Egg Quality, feed by Folium Ginkgo fermented product and Basal diet composition, basal diet is by corn 61.5%, dregs of beans 22%, wheat bran 2%, peanut meal 2%, grease 1%, mountain flour 8.5% and premix 3% composition, Folium Ginkgo fermented product is by ginkgo leaf 42.8%, fermenting microbe 5%, nitrogen source 2.0%, inorganic Salt 0.2% and distilled water 50% ferment, and wherein Folium Ginkgo fermented product is with 0.50% addition of basal diet.
The preparation of Folium Ginkgo fermented product includes the following steps:
S1, prepare raw material: being crushed after ginkgo leaf is air-dried, sieving;
S2, fermenting microbe inoculation: fermenting microbe includes penicillium oxalicum, lactic acid bacteria, bafillus natto, produces Ruan's Candida Bacterium, by four strains, 2:1:2:1 is linked into solid-state fermentation culture medium with 5% inoculum concentration in proportion, penicillium oxalicum × 2: cream Sour bacterium × 1: bafillus natto × 2: producing Ruan saccharomycete × 1, and strain quantity ratio is 2 × 107: 1 × 107: 2 × 107: 1 × 107, It will be inoculated with the bacterium solution that total amount is 5ml on culture medium and carry out mixed fermentation, add cotton plug, triangle bottleneck ox-hide paper bag after being inoculated with Firmly, it is placed in 30 DEG C of constant incubator and cultivates, incubation time 72h;
S3,2.0% nitrogen source is added to triangular flask;
S4,0.2% inorganic salts are added to triangular flask;
S5, fermentation: to triangular flask, adjusting material-water ratio is 1:1 for addition ginkgo leaf and distilled water, and distilled water is 25m l, setting Cultivation temperature is 30 DEG C, and ferment 72h.
Nitrogen source is any one of urea, ammonium sulfate, ammonium nitrate and four kinds of ammonium oxalate.
Inorganic salts are disodium hydrogen phosphate, sodium dihydrogen phosphate, dipotassium hydrogen phosphate, potassium dihydrogen phosphate, calcium chloride and calcium carbonate six Any one of kind.
By three groups of experimental groups of the feed prepared by the present invention containing Folium Ginkgo fermented product and basal diet feeding in the prior art One group of experimental group of material compares experiment, and the experiment periods of this comparative experiments are 60 days, and wherein the preparation of feed uses weight generation For method, in different experimental groups, the Folium Ginkgo fermented product of equivalent is substituted with the wheat bran of same weight.
By comparative experiments it is found that influence experiment table 1 of the feed containing Folium Ginkgo fermented product to performance in layers is united It counts as follows:
Influence of 1 Folium Ginkgo fermented product of table to performance in layers
Project Basal diet 0.20% 0.35% 0.50% SEM2 P value
Laying rate % 84.45 85.05 85.22 85.76 0.088 0.063
Average egg weight g 62.25 62.43 63.14 63.31 0.074 0.065
Daily ingestion amount g 138.88 139.15 138.58 138.16 0.254 0.054
Feedstuff-egg ratio 2.23a 2.23a 2.19b 2.18b 0.026 0.035
Soft rate of broken eggs % 0.78a 0.63ab 0.52b 0.56b 0.016 0.043
Death and culling rate % 3.35a 2.67b 1.68c 1.67c 0.002 0.001
Note: colleague's data upper right corner lowercase difference indicates significant difference (P < 0.05).
It is shown by Statistical Comparison, the feed containing different proportion Folium Ginkgo fermented product is to laying rate, average egg weight and day Feed intake does not make significant difference (P > 0.05), but compared with basal diet group, the feed containing different proportion Folium Ginkgo fermented product Experimental group laying rate and average egg weight have different degrees of improvement;Folium Ginkgo fermented product additive amount is 0.35% and 0.50% The feedstuff-egg ratio of experimental group be significantly improved (P < 0.05);In addition, the experiment of the feed containing different proportion Folium Ginkgo fermented product The soft rate of broken eggs of group and death and culling rate have significant decrease (P < 0.05 or P < 0.01).Illustrate that Folium Ginkgo fermented product can be improved laying hen Laying rate and average egg weight reduce the soft rate of broken eggs and death and culling rate of laying hen, on the daily ingestion amount of laying hen without influence.
By comparative experiments it is found that influence experiment table 2 of the feed containing Folium Ginkgo fermented product to Egg Quality counts as follows:
Influence of 2 Folium Ginkgo fermented product of table to Egg Quality
Project Basal diet 0.20% 0.35% 0.50% SEM2 P value
Egg shape index 1.31 1.33 1.31 1.33 0.051 0.065
Eggshell strength 3.89b 4.31a 4.35a 4.35a 0.135 0.042
Yolk color 8.12 8.19 8.16 8.21 0.012 0.059
Shell thickness 0.411b 0.422ab 0.439a 0.439a 0.003 0.041
Hough unit 86.5 86.9 87.5 86.9 0.596 0.075
Note: colleague's data upper right corner lowercase difference indicates significant difference (P < 0.05).
It is shown by Statistical Comparison, the feed containing different proportion Folium Ginkgo fermented product is equal to egg shape index and Hough unit It does not make significant difference, the feed containing different proportion Folium Ginkgo fermented product is significantly increased to eggshell strength and shell thickness, contains Having the feed of different proportion Folium Ginkgo fermented product has improvement to yolk color.
The basic principles, main features and advantages of the present invention have been shown and described above.The technology of the industry For personnel it should be appreciated that the present invention is not limited to the above embodiments, described in the above embodiment and specification is only the present invention Preference, be not intended to limit the invention, without departing from the spirit and scope of the present invention, the present invention also has various Changes and improvements, these changes and improvements all fall within the protetion scope of the claimed invention.The claimed scope of the invention is by institute Attached claims and its equivalent thereof.

Claims (4)

1. a kind of feed containing Folium Ginkgo fermented product for improving laying hen performance and Egg Quality, it is characterised in that: the feed by Folium Ginkgo fermented product and basal diet composition, the basal diet is by corn 61.5%, dregs of beans 22%, wheat bran 2%, peanut meal 2%, the composition of grease 1%, mountain flour 8.5% and premix 3%, the Folium Ginkgo fermented product is by ginkgo leaf 42.8%, zymophyte Kind 5%, nitrogen source 2.0%, inorganic salts 0.2% and distilled water 50% ferment, and wherein Folium Ginkgo fermented product is respectively with basic day 0.20%, 0.35% and 0.50% addition of grain.
2. a kind of feed containing Folium Ginkgo fermented product for improving laying hen performance and Egg Quality according to claim 1, Be characterized in that: the preparation of the Folium Ginkgo fermented product includes the following steps:
S1, prepare raw material: being crushed after ginkgo leaf is air-dried, sieving;
S2, fermenting microbe inoculation: fermenting microbe includes penicillium oxalicum, lactic acid bacteria, bafillus natto, produces Ruan's candidiasis, By four strains, 2:1:2:1 is linked into solid-state fermentation culture medium with 5% inoculum concentration in proportion, penicillium oxalicum × 2: lactic acid bacteria × 1: bafillus natto × 2: producing Ruan saccharomycete × 1, and strain quantity ratio is 2 × 107: 1 × 107: 2 × 107: 1 × 107, will train It supports the bacterium solution that inoculation total amount is 5ml on base and carries out mixed fermentation, after being inoculated with plus cotton plug, triangle bottleneck are encased with brown paper, It is placed in 30 DEG C of constant incubator and cultivates, incubation time 72h;
S3,2.0% nitrogen source is added to triangular flask;
S4,0.2% inorganic salts are added to triangular flask;
S5, fermentation: to triangular flask, adjusting material-water ratio is 1:1, distilled water 25ml, setting culture for addition ginkgo leaf and distilled water Temperature is 30 DEG C, and ferment 72h.
3. a kind of feed containing Folium Ginkgo fermented product for improving laying hen performance and Egg Quality according to claim 1, Be characterized in that: the nitrogen source is any one of urea, ammonium sulfate, ammonium nitrate and four kinds of ammonium oxalate.
4. a kind of feed containing Folium Ginkgo fermented product for improving laying hen performance and Egg Quality according to claim 1, Be characterized in that: the inorganic salts are disodium hydrogen phosphate, sodium dihydrogen phosphate, dipotassium hydrogen phosphate, potassium dihydrogen phosphate, calcium chloride and carbonic acid Any one of six kinds of calcium.
CN201811175446.2A 2018-10-10 2018-10-10 A kind of feed containing Folium Ginkgo fermented product improving laying hen performance and Egg Quality Pending CN109170336A (en)

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Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102318743A (en) * 2011-09-30 2012-01-18 南京林业大学 Method for improving ginkgo leaf feeding palatability and increasing product bioactivity
CN103392920A (en) * 2013-08-19 2013-11-20 青岛农业大学 Fermentation method of soybean hulls
CN108013263A (en) * 2018-01-17 2018-05-11 霍邱县科瑞达禽业有限公司 A kind of preparation method for the chicken feed additive for adding wolfberry fruit powder

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102318743A (en) * 2011-09-30 2012-01-18 南京林业大学 Method for improving ginkgo leaf feeding palatability and increasing product bioactivity
CN103392920A (en) * 2013-08-19 2013-11-20 青岛农业大学 Fermentation method of soybean hulls
CN108013263A (en) * 2018-01-17 2018-05-11 霍邱县科瑞达禽业有限公司 A kind of preparation method for the chicken feed additive for adding wolfberry fruit powder

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
张莹: "银杏叶生物饲料在蛋鸡饲料中的应用", 《扬州大学硕士学位论文》 *

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Inventor after: Yang Xiaohu

Inventor after: Zhang Xuhui

Inventor after: Cao Fuliang

Inventor before: Yang Xiaohu

Inventor before: Zhang Xuhui

Inventor before: Cao Fuliang

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Application publication date: 20190111