CN109158400B - Treatment process of leather solid waste - Google Patents
Treatment process of leather solid waste Download PDFInfo
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- CN109158400B CN109158400B CN201810790878.8A CN201810790878A CN109158400B CN 109158400 B CN109158400 B CN 109158400B CN 201810790878 A CN201810790878 A CN 201810790878A CN 109158400 B CN109158400 B CN 109158400B
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Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09B—DISPOSAL OF SOLID WASTE NOT OTHERWISE PROVIDED FOR
- B09B3/00—Destroying solid waste or transforming solid waste into something useful or harmless
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09B—DISPOSAL OF SOLID WASTE NOT OTHERWISE PROVIDED FOR
- B09B5/00—Operations not covered by a single other subclass or by a single other group in this subclass
Landscapes
- Engineering & Computer Science (AREA)
- Environmental & Geological Engineering (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Processing Of Solid Wastes (AREA)
Abstract
The invention discloses a treatment process of leather solid waste, which comprises the following steps: 1) removing calcium by using ammonium chloride, 2) crushing, 3) sequentially performing cellulase enzymolysis, protease enzymolysis and lipase enzymolysis, 4) microalgae pre-planting and 5) microalgae putting. The treatment process effectively utilizes the nutrient substances of the leather curing waste as the carbon source and the nitrogen source for the growth of the microalgae, and utilizes the biological curing and biodegradation effects of the microalgae, thereby effectively reducing the content of hexavalent chromium in the waste.
Description
Technical Field
The invention relates to the technical field of industrial three-waste treatment, in particular to a treatment process of leather solid waste.
Background
With the rapid development of industrial economy in China, huge environmental pollution is brought inevitably, and soil, water quality and air quality are greatly reduced due to pollution sources including heavy metals, waste water, waste residues, dust particles and the like. Soil and water contamination was the first concern because these contaminations were visible to the naked eye; air pollution has also been known in recent years, and the concept of PM2.5 is keen. Only heavy metal pollution, because the incubation period is long and the related knowledge is not popularized, the method does not attract the general attention of people.
Heavy metal pollution is a problem that cannot be ignored any more, and serious ecological health risks are brought. Generally, the most direct harm is to cause the change of functions and structures of biological communities (such as fishes, shrimps, plants and the like) in the area and destroy the ecological balance of related water areas and soil. Secondly, heavy metals can enter plant cells through plant roots, which not only affects plant photosynthesis, respiration and nutrition metabolism, but also can enter human bodies through the channels of plant foods, which can cause adverse effects on the health of the human bodies and even affect life activities. Taking rice eaten by people every day as an example, the rice sampling inspection of the university in Zhongshan in 2010 shows that the cadmium exceeding rate of 21 rice varieties respectively reaches 100%. The rice monitoring center of the Ministry of agriculture of China also carries out sampling inspection on rice in the market of China, and the standard exceeding rate of cadmium also reaches 10.3 percent, while pollution sources mainly comprise non-ferrous metal smelting, electroplating, batteries and leather manufacturing industries. Research shows that 95% of heavy metals entering human bodies can be discharged out of the bodies along with digestive waste, and residual heavy metals can cause chronic poisoning after being accumulated for decades.
The heavy metal pollution condition in China is not optimistic and is mainly reflected in the aspects of water pollution and soil pollution. According to statistics, the contents of total copper, total lead and total cadmium in the sediments of rivers, lakes and reservoirs of more than 80 percent of China are in a mild or moderate pollution level, and the contents of lead, copper, mercury and cadmium in coastal sea areas also exceed the standard. Heavy metal pollution of soil is also severe, about 1000 hectares of damage occur, and the yield of grains is directly lost by about 100 hundred million kilograms per year.
Heavy metals have the characteristics of stable property and difficult degradation, pollution caused by the heavy metals is difficult to treat, and a common treatment idea is to fix the heavy metals in a certain area to prevent the heavy metals from diffusing. Commonly used methods are zone-sealing, immobilization by chemical binding and immobilization by biosorption. The microorganism has the characteristics of high growth speed, short metabolic cycle and strong environmental adaptability, so that the microorganism has attracted extensive attention of the industry in recent years when being applied to heavy metal pollution treatment, and some bacteria, fungi and microalgae are found to have treatment effect on heavy metal pollution.
The effect of microorganisms on heavy metals can be divided into four forms: extracellular complexation, intracellular accumulation, reduction of heavy metal toxicity and settlement of heavy metals by cell secretions. The complexation process is mainly performed by sugar and saccharide on cell membraneThe adsorption and embedding of polymers such as protein and the like on heavy metals are finished; the accumulation is a process of leading heavy metal to penetrate a cell membrane to enter a cell and gradually accumulate in the cell; intracellular heavy metals may also synthesize metallothionein with proteins to produce low-or non-toxic compounds; sedimentation is the precipitation of heavy metals bound by cells secreting some acids or enzymes. For example, Chlorococcus has an adsorption effect on heavy metals such as mercury, cadmium and lead, and when the concentration of these heavy metals in the wastewater is 5-20 mg L-1(cadmium and lead) 40-100 mg L-1When the content of the heavy metals in the sewage is within the range of mercury, the growth vigor of the microalgae is good, and the adsorption rates of the heavy metals respectively reach 97 percent (mercury), 86 percent (cadmium) and 70 percent (lead), so that the content of the heavy metals in the sewage is effectively reduced.
China is a big country for leather processing, a chromium tanning agent (chromium powder), chromium-containing wet blue and chromium-containing pigment are used in the tanning process of leather, and a large amount of heavy metal chromium is remained in waste water and waste residues generated in the processing. Hexavalent chromium is one of the 17 well-known highly dangerous toxic heavy metals, and entering the human body may cause kidney damage until the kidney is necrotic.
For the treatment of hexavalent chromium in leather industrial waste residues, known reports or application methods include a physical adsorption method, an electrolytic method, a chemical reduction method, a membrane separation method and a biological flocculation method. Physical adsorption method has strong dependence on environment acidity and alkalinity, and available adsorbents include active carbon, cellulose, sepiolite, resin and the like. For example, Gupta et al report that absorption of hexavalent chromium by sawdust is optimal under extremely acidic conditions (pH 1) to 41.5mg g-1Higher adsorption rates are achieved, however such acidic conditions increase the risk of secondary environmental pollution and may have the consequence of a large accumulation of sawdust. The point decomposition method also needs to convert hexavalent chromium into trivalent chromium under the action of an electrode under an acidic condition so as to reduce the toxicity of the hexavalent chromium. The membrane separation method is to perform physical separation according to the molecular size difference of different substances, and currently, complete equipment is available, but the method needs to perform fine pretreatment on sewage to ensure that the separation membrane is not blocked, and the separation membrane has a limited service life and needs to be replaced regularly.
The microorganisms used in the flocculation of hexavalent chromium organisms, such as Agrobacterium and Staphylococcus, and white rot fungus and yeast, have been reported to have a flocculation rate of up to 42%. In the current reports, no microalgae species with good adsorption effect on hexavalent chromium is found, and chlorella, synechococcus, spirulina, crescent moon algae, scenedesmus and the like can not grow well in water areas containing hexavalent chromium. The treatment methods are used for treating hexavalent chromium in liquid, but no relevant report is found for treating hexavalent chromium in solid waste.
The solid waste from leather processing comprises waste hair, meat membrane, broken leather, leftover materials, leather scraps and the like, and the waste components generating pressure to the environment mainly comprise biological materials such as collagen, fat, fiber, carbohydrate and the like, and heavy metal residues for processing such as metal chromium and the like.
Disclosure of Invention
In order to overcome the disadvantages of the prior art, the invention aims to provide a treatment process of biochemically absorbed skin or solidified waste.
The purpose of the invention is realized by adopting the following technical scheme:
a treatment process of leather solid waste comprises the following steps:
1) calcium removal: soaking the leather curing waste in an ammonium chloride solution, fishing out, leaching and drying;
2) crushing: crushing the solid waste subjected to calcium removal in the step 1) to obtain powdery waste;
3) biological enzymolysis: suspending the powdery waste in a carbonic acid solution with the pH value of 3.5-5.5 according to the feed-liquid ratio of 1 (7-10), sequentially performing enzymolysis by cellulase and protease, adding sodium carbonate to adjust the pH value to 7-9, and then adding lipase for enzymolysis to obtain an enzymolysis solution;
4) pre-planting microalgae: mixing Euglena gracilis at a ratio of (0.5-1.5) x 109Placing the inoculum size of each 100mL into 50-150mL of culture solution for culturing, and adding 50-150mL of enzymolysis solution obtained in the step 3) to obtain a pre-planting solution;
5) putting microalgae: placing the enzymolysis liquid obtained in the step 3) in an open reaction tank, and placing the pre-planting liquid obtained in the step 4) at the ratio of (0.8-1.4) x 1012Per m3Inoculating the supernatant into the open reaction tank, reacting for 3-6 days under aeration, stopping aeration, settling, performing solid-liquid separation to kill algae, and discharging the supernatant after the supernatant is qualified.
Further, in the step 1), the concentration of the ammonium chloride solution is 1-2 wv%.
Further, in the step 2), a colloid mill is used for grinding until the granularity is less than or equal to 0.25 cm.
Further, the step 3) of biological enzymolysis specifically comprises the following steps:
a) suspending the powdery waste in a carbonic acid solution with the pH value of 3.5-5.5 according to the material-liquid ratio of 1 (7-10), adding cysteine, stirring, adding cellulase, and performing enzymolysis for 2-8 h;
b) adding carbonic acid until the pH value is 3.5-5.5, and adding protease for enzymolysis for 0.5-4 h;
c) adding sodium carbonate to adjust pH to 7-9, adding lipase for enzymolysis for 2-6 hr to obtain enzymolysis solution.
Further, in the step 3), the enzyme activity concentration of the cellulase is more than 1.8U/g of feed liquid, and the enzymolysis temperature is 20-35 ℃.
Further, in the step 3), the protease is bromelain, the enzyme activity is more than 0.5U/g of feed liquid, and the enzymolysis temperature is 20-35 ℃.
Further, in the step 3), the enzyme activity of the lipase is more than 1.2U/g of feed liquid, and the enzymolysis temperature is 20-35 ℃.
Further, in the step 4), the euglena gracilis is cultured in Hunter trace element culture solution.
Further, in the step 5), the feed liquid is subjected to secondary filtration and sedimentation.
Further, in the step 5), the specific operation of secondary filtration and sedimentation is as follows: discharging the feed liquid to a conical sedimentation tank through a filter screen with the aperture of 0.5cm, recovering euglena gracilis from the sediment, discharging the supernatant to a conical secondary sedimentation tank through a filter screen with the aperture of 0.2cm, adding 0.01-0.06ppm of algicide, and overflowing the supernatant.
Compared with the prior art, the invention has the beneficial effects that:
aiming at leather processing solid waste, the invention combines a physical crushing method, an enzymolysis method and a microbial flocculation method to establish a method for recycling waste residues, the method is used as a nutrient source of microalgae to culture the microalgae, so that when components such as fat, protein and the like are digested, hexavalent chromium is also converted into nontoxic trivalent chromium, and the produced microalgae can be subjected to subsequent recycling;
according to the treatment process of the leather or the solidified waste, provided by the invention, the biochemical fixing effect of euglena gracilis is utilized, the nitrogen source and the carbon source which can absorb protein, fat and cellulose of the leather waste and can grow per se are utilized, microalgae cells metabolize to produce high-value nutrient components and chemical raw materials such as polysaccharide, vitamin, beta-carotene and the like, and harmful substances such as heavy metals and the like in the leather waste are effectively fixed, so that the total chromium and hexavalent chromium in the treated water body and precipitates can reach the emission standard; in the treatment process, no biological harmful reagent is used, so that the environmental pressure can be effectively reduced.
Detailed Description
The present invention is further described below with reference to specific embodiments, and it should be noted that, without conflict, any combination between the embodiments or technical features described below may form a new embodiment.
The invention provides a treatment process of leather solid waste, which comprises the following steps:
1) calcium removal: soaking the leather curing waste in an ammonium chloride solution, fishing out, leaching and drying;
the calcium removal treatment provides a relatively proper pH value adaptability and biological adaptability environment for subsequent enzymolysis;
2) crushing: crushing the solid waste subjected to calcium removal in the step 1) to obtain powdery waste;
crushing can effectively improve the dissolving-out capability of heavy metal on one hand and can effectively improve the contact area of enzyme and waste on the other hand;
3) biological enzymolysis: suspending the powdery waste in a carbonic acid solution with the pH value of 3.5-5.5 according to the feed-liquid ratio of 1 (7-10), sequentially performing enzymolysis by cellulase and protease, adding sodium carbonate to adjust the pH value to 7-9, and then adding lipase for enzymolysis to obtain an enzymolysis solution;
in the step, main biological nutrients in the waste are effectively enzymolyzed to provide a nitrogen-carbon source for the subsequent microalgae propagation,
4) pre-planting microalgae: culturing Euglena gracilis in culture medium at (0.5-1.5) x 109Transferring the inoculum size of each 100mL into 50-150mL of the enzymolysis liquid obtained in the step 3) to obtain a pre-planting liquid;
5) putting microalgae: placing the enzymolysis liquid obtained in the step 3) in an open reaction tank, and placing the pre-planting liquid obtained in the step 4) at the ratio of (0.8-1.4) x 1012Per m3Inoculating the supernatant into the open reaction tank, reacting for 3-6 days under aeration, stopping aeration, settling, performing solid-liquid separation to kill algae, and discharging the supernatant after the supernatant is qualified.
Compared with the traditional adsorption method or electrolysis method, the treatment process does not need to be carried out under the condition of extreme acidity (pH is less than or equal to 3.0); compared with the emerging membrane separation method, the method has the advantages of low investment, low carbon and environmental protection; compared with the fungus flocculation method, the recovered microalgae contains more beneficial components, can be simply processed into waste materials, feeds or fishery baits, and can also be deeply processed to extract components such as protein, polysaccharide, lipid and the like in cells.
The treatment process has short treatment period, one treatment period is about 6 days, the treatment process can be carried out discontinuously or continuously, two emissions, namely sludge and water, are generated during treatment, the main component of the sludge is algae and can be recycled, and the water reaches the water quality discharge standard of the industry.
In the treatment process, one part of harmful heavy metal chromium is biologically solidified by euglena gracilis through biological solidification, and the other part of harmful heavy metal chromium is reduced into nontoxic trivalent chromium which can be discharged.
In the following embodiments, the starting materials described are all available by commercially available means, and the unit "wv%" below is a unit containing a solute in a predetermined weight per 100mL of a solvent by volume, unless otherwise specified.
Example 1:
1) calcium removal: soaking 4.2kg of the leather curing waste in 10L of 1 wv% ammonium chloride solution for 5h, taking out, leaching with 40L of water, and drying;
2) crushing: crushing the solid waste subjected to calcium removal in the step 1) to obtain powdery waste, wherein the particle size of the powdery waste is less than or equal to 0.25 cm;
3) biological enzymolysis: charging carbonic acid into deionized water to make pH value 3.5 to obtain carbonic acid solvent, adding 7kg of carbonic acid solvent into 1kg of powdery waste, adding 0.7g of cysteine, stirring, adding 8.82kU of cellulase, and performing enzymolysis at 30 ℃ for 2 h; adding carbonic acid into each carbonic acid solution until the pH value is 3.5, adding bromelain of 3.5kU, and performing enzymolysis for 0.5h at 30 ℃; adding sodium carbonate to adjust the pH to 8.3, adding 8.4kU lipase for enzymolysis at 30 ℃ for 2h to obtain an enzymolysis solution;
4) pre-planting microalgae: culturing Euglena gracilis in 50mL Hunter microelement culture solution, inoculating 0.5 × 109Culturing euglena cellules for 3 days, adding 50mL of the enzymolysis liquid obtained in the step 3), culturing for 2 weeks, adding 10L of the enzymolysis liquid obtained in the step 3), and culturing under an open outdoor condition, wherein a culture solution which is not sensed by bacteria is a pre-planting solution;
5) putting microalgae: placing the enzymolysis liquid obtained in the step 3) in an open reaction tank, and mixing the pre-planting liquid obtained in the step 4) with the weight of 0.8 multiplied by 1012Per m3Inoculating the mixture into the open reaction tank, reacting for 4d under aeration, and stopping aeration; discharging the feed liquid to a conical sedimentation tank through a filter screen with the aperture of 0.5cm after 2h, staying for 5h, recovering euglena gracilis and nutrient components from the sediment, discharging the supernatant to a conical secondary sedimentation tank through a filter screen with the aperture of 0.2cm, adding 0.01ppm of algicide, stirring and staying for 14h, overflowing the supernatant, and discharging after the supernatant is inspected and qualified.
Example 2:
1) calcium removal: soaking 4.45kg of the leather curing waste in 10L of 1.5 wv% ammonium chloride solution for 5h, taking out, leaching with 40L of water, and drying;
2) crushing: crushing the solid waste subjected to calcium removal in the step 1) to obtain powdery waste, wherein the particle size of the powdery waste is less than or equal to 0.25 cm;
3) biological enzymolysis: charging carbonic acid into deionized water to make pH 4.5 to obtain carbonic acid solvent, adding 8kg of carbonic acid solvent into 1kg of powdery waste, adding 0.9g of cysteine, stirring, adding 24kU of cellulase, and performing enzymolysis at 30 ℃ for 2 h; adding carbonic acid into each carbonic acid solution until the pH value is 4.5, and adding 80kU of bromelain for enzymolysis for 0.5h at 30 ℃; adding sodium carbonate to adjust the pH to 8.3, adding 48kU lipase for enzymolysis at 30 ℃ for 2h to obtain an enzymolysis solution;
4) pre-planting microalgae: culturing Euglena gracilis in 100mL Hunter microelement culture solution, inoculating 1 × 109Culturing euglena cellules for 3 days, adding the euglena cellules into 100mL of enzymolysis liquid obtained in the step 3), culturing for 2 weeks, adding 15L of enzymolysis liquid obtained in the step 3), and culturing under an open outdoor condition, wherein a culture solution which is not sensed by bacteria is a pre-planting solution;
5) putting microalgae: placing the enzymolysis liquid obtained in the step 3) in an open reaction tank, and mixing the pre-planting liquid obtained in the step 4) with the weight of 1.0 multiplied by 1012Per m3Inoculating the mixture into the open reaction tank, reacting for 5d under aeration, and stopping aeration; discharging the feed liquid to a conical sedimentation tank through a filter screen with the aperture of 0.5cm after 2h, staying for 7h, recovering euglena gracilis and nutrient components from the sediment, discharging the supernatant to a conical secondary sedimentation tank through a filter screen with the aperture of 0.2cm, adding 0.01ppm of algicide, stirring and staying for 14h, overflowing the supernatant, and discharging after the supernatant is inspected and qualified.
Example 3:
1) calcium removal: soaking 4.0kg of the leather curing waste in 10L of 2wv% ammonium chloride solution for 5h, taking out, leaching with 40L of water, and drying;
2) crushing: crushing the solid waste subjected to calcium removal in the step 1) to obtain powdery waste, wherein the particle size of the powdery waste is less than or equal to 0.25 cm;
3) biological enzymolysis: charging carbonic acid into deionized water to make pH 5.5 to obtain carbonic acid solvent, adding 10kg of carbonic acid solvent into 1kg of powdery waste, adding 1.2g of cysteine, stirring, adding 70kU of cellulase, and performing enzymolysis at 30 ℃ for 2 h; adding carbonic acid into each carbonic acid solution until the pH value is 5.5, and adding 120kU of bromelain for enzymolysis for 0.5h at 30 ℃; adding sodium carbonate to adjust the pH to 8.0, adding 78kU lipase for enzymolysis at 30 ℃ for 2h to obtain an enzymolysis solution;
4) pre-planting microalgae: culturing Euglena gracilis in 150mL Hunter microelement culture solution, inoculating 1 × 109Individual fiber bareCulturing algae cells for 3 days, transferring to 150mL of the enzymolysis liquid obtained in the step 3), culturing for 2 weeks, adding into 20L of the enzymolysis liquid obtained in the step 3), and culturing under open outdoor conditions, wherein the culture solution which is not sensed by bacteria is a pre-planting solution;
5) putting microalgae: placing the enzymolysis liquid obtained in the step 3) in an open reaction tank, and mixing the pre-planting liquid obtained in the step 4) with the concentration of 1.4 multiplied by 1012Per m3Inoculating the mixture into the open reaction tank, reacting for 5d under aeration, and stopping aeration; discharging the feed liquid to a conical sedimentation tank through a filter screen with the aperture of 0.5cm after 2h, staying for 8h, recovering euglena gracilis and nutrient components from the sediment, discharging the supernatant to a conical secondary sedimentation tank through a filter screen with the aperture of 0.2cm, adding 0.01ppm of algicide, stirring and staying for 16h, overflowing the supernatant, and discharging after the supernatant is inspected and qualified.
Comparative example 1:
comparative example 1 unlike example 1, comparative example 1 uses chlorella.
Detection examples
The quality of the supernatant and the precipitate obtained in examples 1 to 3 was measured according to the emission Standard of Water pollutants for leather and fur processing industry (GB30486-2013) as shown in tables 1 and 2:
TABLE 1 supernatant test data
TABLE 2 sediment assay data
| Example 1 | Example 2 | Example 3 | Comparative example 1 | |
| Total chromium mg/L | 0.68 | 0.47 | 0.43 | 2.46 |
| Cr6+mg/L | 0.079 | 0.066 | 0.043 | 1.67 |
| pH value | 7.7 | 7.2 | 7.3 | 7.3 |
As can be seen from Table 1, the indexes of the supernatant treated in examples 1-3 all reach the discharge standard of leather wastewater, and the contents of chromium and hexavalent chromium in the precipitate are low.
The above embodiments are only preferred embodiments of the present invention, and the protection scope of the present invention is not limited thereby, and any insubstantial changes and substitutions made by those skilled in the art based on the present invention are within the protection scope of the present invention.
Claims (10)
1. A treatment process of leather solid waste is characterized by comprising the following steps:
1) calcium removal: soaking the leather solid waste in an ammonium chloride solution, fishing out, leaching and drying;
2) crushing: crushing the leather solid waste subjected to calcium removal in the step 1) to obtain powdery waste;
3) biological enzymolysis: suspending the powdery waste in a carbonic acid solution with the pH value of 3.5-5.5 according to the material-liquid ratio of 1 (7-10), sequentially performing enzymolysis by cellulase and bromelain, adding sodium carbonate to adjust the pH value to 7-9, and adding lipase for enzymolysis to obtain an enzymolysis solution;
4) pre-planting microalgae: mixing Euglena gracilis at a ratio of (0.5-1.5) x 109Placing the inoculum size of each 100mL into 50-150mL of culture solution for culture, transferring into 50-150mL of enzymolysis solution obtained in the step 3) after culture, and obtaining pre-planting solution;
5) putting microalgae: placing the enzymolysis liquid obtained in the step 3) in an open reaction tank, and taking euglena gracilis of (0.8-1.4) x 10 as the pre-planting liquid obtained in the step 4)12Per m3Inoculating the supernatant into the open reaction tank, reacting for 3-6d under aeration, stopping aeration, settling, performing solid-liquid separation to kill algae, and discharging the supernatant after the supernatant is qualified;
the leather solid waste comprises a biomaterial and hexavalent chromium, wherein the biomaterial comprises collagen, fat and cellulose.
2. The process for treating leather solid waste according to claim 1, wherein the concentration of the ammonium chloride solution in step 1) is 1 to 2 wv%.
3. The process for treating leather solid waste material according to claim 1, wherein in the step 2), the leather solid waste material is pulverized to a particle size of 0.25cm or less using a colloid mill.
4. The process for treating leather solid wastes according to claim 1, wherein the step 3) of biological enzymatic hydrolysis comprises the following steps:
a) suspending the powdery waste in a carbonic acid solution with the pH value of 3.5-5.5 according to the material-liquid ratio of 1 (7-10), adding cysteine, stirring, adding cellulase, and performing enzymolysis for 2-8 h;
b) adding carbonic acid until the pH value is 3.5-5.5, adding bromelain for enzymolysis for 0.5-4 h;
c) adding sodium carbonate to adjust pH to 7-9, adding lipase for enzymolysis for 2-6 hr to obtain enzymolysis solution.
5. The process for treating leather solid wastes according to claim 4, wherein in the step 3), the enzyme activity concentration of the cellulase is more than 1.8U/g of feed liquid, and the enzymolysis temperature is 20-35 ℃.
6. The process for treating leather solid wastes according to claim 4, wherein in the step 3), the bromelain enzyme activity concentration is more than 0.5U/g of feed liquid, and the enzymolysis temperature is 20-35 ℃.
7. The process for treating leather solid wastes according to claim 4, wherein in the step 3), the enzyme activity concentration of the lipase is more than 1.2U/g of feed liquid, and the enzymolysis temperature is 20-35 ℃.
8. The process for treating leather solid waste material according to claim 1, wherein in the step 4), Euglena gracilis is cultured in Hunter's microelement culture solution.
9. The process for treating leather solid wastes according to claim 1, wherein in the step 5), the feed liquid is settled by secondary filtration.
10. The process for treating leather solid wastes according to claim 9, wherein the secondary filtering and settling in step 5) is carried out by the following steps: discharging the feed liquid to a conical sedimentation tank through a filter screen with the aperture of 0.5cm, recovering euglena gracilis from the sediment, discharging the supernatant to a conical secondary sedimentation tank through a filter screen with the aperture of 0.2cm, adding 0.01-0.06ppm of algicide, and overflowing the supernatant.
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