CN109153956B - 用于高效将分子递送至单细胞中的高通量反馈控制型电穿孔微型装置 - Google Patents

用于高效将分子递送至单细胞中的高通量反馈控制型电穿孔微型装置 Download PDF

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CN109153956B
CN109153956B CN201680061897.6A CN201680061897A CN109153956B CN 109153956 B CN109153956 B CN 109153956B CN 201680061897 A CN201680061897 A CN 201680061897A CN 109153956 B CN109153956 B CN 109153956B
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signal
permeabilization
cell
impedance value
threshold
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CN109153956A (zh
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杰弗里·扎恩
郑明德
大卫·I·施瑞博尔
林灏
单维正
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Rutgers, The State University of New Jersey
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    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/42—Apparatus for the treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
    • B—PERFORMING OPERATIONS; TRANSPORTING
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    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads or physically stretching molecules
    • C—CHEMISTRY; METALLURGY
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    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/16—Microfluidic devices; Capillary tubes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/02—Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N13/00—Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
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    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/1023—Microstructural devices for non-optical measurement
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/1031—Investigating individual particles by measuring electrical or magnetic effects
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B01L2300/0645—Electrodes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N2015/1006—Investigating individual particles for cytology

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  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
CN201680061897.6A 2015-09-04 2016-09-02 用于高效将分子递送至单细胞中的高通量反馈控制型电穿孔微型装置 Active CN109153956B (zh)

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Application Number Priority Date Filing Date Title
US201562214665P 2015-09-04 2015-09-04
US62/214,665 2015-09-04
PCT/US2016/050201 WO2017040995A1 (en) 2015-09-04 2016-09-02 High throughput, feedback-controlled electroporation microdevice for efficient molecular delivery into single cells

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CN109153956B true CN109153956B (zh) 2022-10-28

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US (2) US10927333B2 (cg-RX-API-DMAC7.html)
EP (1) EP3344746B1 (cg-RX-API-DMAC7.html)
JP (1) JP6989133B2 (cg-RX-API-DMAC7.html)
KR (2) KR20180042422A (cg-RX-API-DMAC7.html)
CN (1) CN109153956B (cg-RX-API-DMAC7.html)
AU (1) AU2016315883B2 (cg-RX-API-DMAC7.html)
BR (1) BR112018004278B1 (cg-RX-API-DMAC7.html)
CA (1) CA2997435C (cg-RX-API-DMAC7.html)
WO (1) WO2017040995A1 (cg-RX-API-DMAC7.html)

Families Citing this family (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3440186A4 (en) 2016-04-04 2019-12-11 Cytequest, Inc. SYSTEM, APPARATUS AND METHOD FOR THE ELECTROPORATION OF CELLS
WO2019125333A2 (en) * 2017-08-23 2019-06-27 Istanbul Teknik Universitesi Microfluidic system for cancer cell separation, capturing and drug screening assays
CN116987589A (zh) 2017-10-19 2023-11-03 苏州壹达生物科技有限公司 一种流式电穿孔装置
US11485944B2 (en) * 2018-04-04 2022-11-01 Hewlett-Packard Development Company, L.P. Microfluidic cellular membrane modification devices
EP3564355A1 (en) * 2018-05-01 2019-11-06 The Charles Stark Draper Laboratory, Inc. Method and device for exosomes electroporation
WO2020014538A1 (en) * 2018-07-12 2020-01-16 Gpb Scientific, Llc Microfluidic methods for the preparation of cells
WO2020041480A1 (en) * 2018-08-21 2020-02-27 The Regents Of The University Of California High-throughput system and method for the temporary permeabilization of cells using lipid bilayers
WO2020047504A2 (en) * 2018-08-31 2020-03-05 The Charles Stark Draper Laboratory, Inc. Method and apparatus for high throughput high efficiency transfection of cells
CN113677392B (zh) * 2018-10-26 2024-09-17 凯拓盘公司 用于电穿孔的装置、系统和套件及其使用方法
CN112840015A (zh) * 2018-11-12 2021-05-25 飞秒生物医学有限公司 控制细胞内递送物质的方法及装置
CN113613711B (zh) * 2019-02-04 2024-09-17 新泽西州立罗格斯大学 使用微电极进行组织电转移的装置
EP3969580A4 (en) * 2019-05-16 2023-07-19 Cytequest, Inc. ELECTROPORATION DEVICES, METHODS AND SYSTEMS
JP2022540797A (ja) * 2019-07-02 2022-09-20 カイトペン コーポレイション 電気機械的送達のためのデバイス、システム、及びキット、ならびにそれらの使用方法
WO2021015779A1 (en) 2019-07-25 2021-01-28 Hewlett-Packard Development Company, L.P. Cell poration and transfection apparatuses
CN110835603A (zh) * 2019-10-09 2020-02-25 遵义医科大学珠海校区 一种快速实现细胞膜可逆性损伤的装置及方法
EP3871773B1 (en) * 2020-02-27 2025-12-10 Cellix Limited A method of determining the transfection status of a plurality of cells
US12006507B2 (en) 2020-07-22 2024-06-11 Nantcell, Inc. Electroporation with active compensation
WO2022045980A1 (en) * 2020-08-25 2022-03-03 Singapore University Of Technology And Design Device and method for determining a mechanical property of a particle
US20220071692A1 (en) * 2020-09-08 2022-03-10 Biosense Webster (Israel) Ltd. Impedance based irreversible-electroporation (ire)
US12472495B2 (en) * 2020-10-28 2025-11-18 Northwestern University Microfluidic device for live cell manipulation and analysis
US12350682B2 (en) * 2021-03-19 2025-07-08 National Yang Ming Chiao Tung University Microelectrode device, microfluidic chip, and microfluidic examination method
US12630816B2 (en) 2021-10-21 2026-05-19 Hewlett-Packard Development Company, L.P. Alignment and fusion of cells with an electrical field
EP4599040A2 (en) * 2022-10-07 2025-08-13 Rutgers, the State University of New Jersey High throughput, feedback-controlled electroporation microdevice for efficient molecular delivery into single cells
WO2024259237A2 (en) * 2023-06-15 2024-12-19 Georgia Tech Research Corporation Targeted feedback-controlled high efficiency single cell electroporation for gene and cell therapy
WO2025128607A1 (en) * 2023-12-11 2025-06-19 Triple Ring Technologies, Inc. Method and apparatus for high-throughput transfection of cells using electroporation
WO2026015154A1 (en) * 2024-07-12 2026-01-15 Hewlett-Packard Development Company, L.P. Microfluidic device with sensor
WO2026142698A1 (en) * 2024-12-24 2026-07-02 Hewlett-Packard Development Company, L.P. Microfluidic device with sensor

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2003505073A (ja) * 1999-07-21 2003-02-12 ザ・レジェンツ・オブ・ザ・ユニバーシティー・オブ・カリフォルニア 制御されたエレクトロポレーションおよび細胞を介した膜物質輸送
CN101233237A (zh) * 2005-05-26 2008-07-30 加利福尼亚大学董事会 跨组织细胞膜的受控电穿孔和传质
CN102680526A (zh) * 2012-05-16 2012-09-19 清华大学 单细胞阵列微芯片及其制造、电测量和电穿孔方法

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH07184686A (ja) 1993-12-28 1995-07-25 Nec Corp 細胞活性測定方法
US7456012B2 (en) * 1997-11-06 2008-11-25 Cellectricon Ab Method and apparatus for spatially confined electroporation
IT1320187B1 (it) * 2000-04-21 2003-11-26 Igea Srl Dispositivo e metodo di elettro-porazione che fornisce un segnalemodulato sotto il controllo continuo della elettro-permeabilizzazione
US8206903B2 (en) 2002-12-20 2012-06-26 Acea Biosciences Device and method for electroporation-based delivery of molecules into cells and dynamic monitoring of cell responses
US7338796B1 (en) * 2003-08-13 2008-03-04 Sandia Corporation Vesicle-based method and apparatus for collecting, manipulating, and chemically processing trace macromolecular species
WO2005056788A1 (en) * 2003-12-08 2005-06-23 Excellin Life Sciences, Inc. Device and method for controlled electroporation and molecular delivery into cells and tissue
US8058056B2 (en) 2004-03-12 2011-11-15 The Regents Of The University Of California Method and apparatus for integrated cell handling and measurements
WO2006112870A1 (en) * 2005-04-19 2006-10-26 Excellin Life Sciences, Inc. Device and method for controlled electroporation and molecular delivery in cells and tissue
US7951582B2 (en) 2005-12-19 2011-05-31 Yissum Research Development Company Of The Hebrew University Of Jerusalem Systems and methods for analyzing and manipulating biological samples
ITTO20060278A1 (it) 2006-04-13 2007-10-14 Silicon Biosystem S P A Metodo per la selezione e/o il processamento di particelle, in particolare cellule
US7678256B2 (en) * 2006-11-03 2010-03-16 Sandia Corporation Insulator-based DEP with impedance measurements for analyte detection
AU2008213740B2 (en) * 2007-02-08 2013-02-07 Veem Ltd Marine propeller pitch adjustment means

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2003505073A (ja) * 1999-07-21 2003-02-12 ザ・レジェンツ・オブ・ザ・ユニバーシティー・オブ・カリフォルニア 制御されたエレクトロポレーションおよび細胞を介した膜物質輸送
CN101233237A (zh) * 2005-05-26 2008-07-30 加利福尼亚大学董事会 跨组织细胞膜的受控电穿孔和传质
CN102680526A (zh) * 2012-05-16 2012-09-19 清华大学 单细胞阵列微芯片及其制造、电测量和电穿孔方法

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
"Modeling electroporation in a single cell";Wanda Krassowska等;《Biophysical Journal》;20070131;第92卷;第404-417页 *
"微流控技术在细胞转染中的应用";徐海明等;《生物工程学报》;20111025;第27卷(第10期);第1417-1427页 *

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CN109153956A (zh) 2019-01-04
EP3344746A4 (en) 2019-01-16
US20210139825A1 (en) 2021-05-13
CA2997435C (en) 2022-11-08
WO2017040995A1 (en) 2017-03-09
CA2997435A1 (en) 2017-03-09
US10927333B2 (en) 2021-02-23
JP2018529333A (ja) 2018-10-11
KR20180042422A (ko) 2018-04-25
US20180258379A1 (en) 2018-09-13
BR112018004278B1 (pt) 2022-05-03
US11987777B2 (en) 2024-05-21
JP6989133B2 (ja) 2022-01-05
EP3344746B1 (en) 2021-07-21
AU2016315883A1 (en) 2018-03-29
KR20240144409A (ko) 2024-10-02
EP3344746A1 (en) 2018-07-11
AU2016315883B2 (en) 2021-01-28
BR112018004278A2 (cg-RX-API-DMAC7.html) 2018-10-02

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