CN109134675A - A kind of extracting method of apple peel polysaccharide - Google Patents
A kind of extracting method of apple peel polysaccharide Download PDFInfo
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- CN109134675A CN109134675A CN201810748785.9A CN201810748785A CN109134675A CN 109134675 A CN109134675 A CN 109134675A CN 201810748785 A CN201810748785 A CN 201810748785A CN 109134675 A CN109134675 A CN 109134675A
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- apple peel
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
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Abstract
The invention discloses a kind of extracting methods of apple peel polysaccharide, comprising the following steps: pretreatment, degreasing, extraction, filtering, enzymatic hydrolysis, UF membrane, de- albumen, depigmentation and dialysis etc., invention using apple skin after being lyophilized by the way of powder processed.It is crushed more completely after freeze-drying, broken extraction polysaccharide yield more direct than fresh hide is high, and nutrient component damages are few in fresh hide；And apple peel polysaccharide is extracted using enzymatic hydrolysis, centrifugation and ultrasonic extraction and dialysis etc., cellulase and plant rennet energy lysed cells wall, be conducive to the dissolution of polysaccharide, immersion method is extracted using higher Extracting temperature, it can get higher polysaccharide yield, the combination of two kinds of leach extraction methods is extracted, and solves the problems, such as that polysaccharide extract rate is low, and process is easy to operate.
Description
Technical field
The present invention relates to technical field of polysaccharide extraction, the extracting method of specifically a kind of apple peel polysaccharide.
Background technique
Apple is rosaceae Maloideae Malus, deciduous tree.The pericarp of apple is rich in polysaccharide, minerals and dimension
Raw element is one of the fruit of people's most often feeding.Apple is the most common fruit, is rich in nutrition abundant, is four flood of the world
The hat of fruit (apple, grape, citrus and banana).Sugar is one of main active of pineapple bran, and research finds pineapple bran
Polysaccharide has anti-oxidant, anti peroxidation of lipid and antitumor action, while can also improve intestinal environment, inhibit T and B lymph thin
The disease resistance of born of the same parents' proliferation, enhancing weaned piglets.Based on this, if by pineapple bran carry out resource reutilization come prepare polysaccharide for
The added value of pineapple process deeply industry is improved, extends pineapple industry chain, its intensive processing is instructed to have important practical significance.Have
The research report for closing pineapple bran polysaccharide extracting process is mostly water extraction and alcohol precipitation method, and wherein water extraction and alcohol precipitation method is the most biography of Polyose extraction
System method, it is easy to operate, it is at low cost, it is not required to special installation, but recovery rate is lower.
Summary of the invention
The purpose of the present invention is to provide a kind of extracting methods of apple peel polysaccharide, to solve to mention in above-mentioned background technique
Out the problem of.
To achieve the above object, the invention provides the following technical scheme:
A kind of extracting method of apple peel polysaccharide, comprising the following steps:
S1 pretreatment: powder will be beaten after the apple peel of screening is freeze-dried, obtains apple peel dry powder；
S2 degreasing: apple peel dry powder obtained by step S1 is placed in container, and 30~60 DEG C of boiling range of petroleum ether reflux is added and mentions
It takes 1~3 time, collects filter residue, it is spare after natural drying；
S3 is extracted: distilled water is added in the filter residue that dries that step is obtained, and is then handled to obtain extracting solution B with microwave ultrasonic wave instrument；
S4 filtering: extracting solution B is filtered by vacuum, and filtrate A is obtained；
S5 enzymatic hydrolysis: cellulase and plant rennet is added in filtrate A, digests 30-90min at PH5.0 and 40-70 DEG C of water-bath,
Obtain liquor C；
S6 UF membrane: liquor C is fitted into row centrifugation in centrifuge tube, when pressure is 0.2~0.6MPa, carries out film with ultrafiltration membrane
Separation, removes residue after centrifugation to obtain heart liquid；
S7 takes off albumen: adjusting the centrifugate pH to 3 of S6 with 6mol/L hydrogen chloride solution, is extracted with the chloroform of isometric ratio
It takes twice, supernatant is stayed to be filtered, then eluted with sodium chloride solution, it is more to obtain de- albumen for eluent concentration dialysis desalination
Sugar juice D；
S8 depigmentation: being added macroporous absorbent resin into the de- proteoglycan solution D that S7 is obtained, and mixes, 1~3h of shaken at room temperature,
It filters, obtains decoloration polysaccharide solution；
S9 dialysis: the polysaccharide solution that will decolourize reverse flowing water dialysis, last concentrated frozen it is dry apple peel polysaccharide.
As a further solution of the present invention: the pineapple bran dry powder mesh number that the step S1 is pre-processed is less than 60
Mesh.
As further scheme of the invention: the microwave ultrasonic wave instrument that the step S3 is extracted is at 50~90 DEG C of temperature
Lower extraction, and the time extracted is 30 minutes.
As further scheme of the invention: the distilled water and apple peel dry powder being added in the step S3 extraction
Envelope-bulk to weight ratio is 500:1ml/g.
As further scheme of the invention: the step S6 filtrate is centrifuged under the speed of 4000r/min,
Time 30min.
As further scheme of the invention: the molecule interception of bag filter used in the step S9 dialysis is 8-
14kDa。
As further scheme of the invention: centrifugate is obtained in the step S6 UF membrane needs 5-8 DEG C of placement 2h.
Compared with prior art, the beneficial effects of the present invention are: the present invention is after apple skin freeze-drying by the way of powder processed.Freeze
It is crushed more completely after dry, broken extraction polysaccharide yield more direct than fresh hide is high, and nutrient component damages are few in fresh hide；And use enzyme
Solution, centrifugation and ultrasonic extraction and dialysis etc. extract apple peel polysaccharide, cellulase and plant rennet energy lysed cells wall,
Be conducive to the dissolution of polysaccharide, immersion method is extracted using higher Extracting temperature, can get higher polysaccharide yield, two kinds of leachings
The combination for proposing method is extracted, and solves the problems, such as that polysaccharide extract rate is low, and process is easy to operate.
Detailed description of the invention
Fig. 1 is the polysaccharide yield histogram that various mould substances are extracted.
Specific embodiment
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete
Site preparation description, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.It is based on
Embodiment in the present invention, it is obtained by those of ordinary skill in the art without making creative efforts every other
Embodiment shall fall within the protection scope of the present invention.
Embodiment one:
A kind of extracting method of apple peel polysaccharide, comprising the following steps:
S1 pretreatment: powder will be beaten after the apple peel of screening is freeze-dried, obtains apple peel dry powder；
S2 degreasing: apple peel dry powder obtained by step S1 is placed in container, and 30 DEG C of boiling range of petroleum ether refluxing extraction 2 is added
It is secondary, filter residue is collected, it is spare after natural drying；
S3 is extracted: distilled water is added in the filter residue that dries that step is obtained, and is then handled to obtain extracting solution B with microwave ultrasonic wave instrument；
S4 filtering: extracting solution B is filtered by vacuum, and filtrate A is obtained；
S5 enzymatic hydrolysis: cellulase and plant rennet is added in filtrate A, digests 30min under PH5.0 and 40 DEG C of water-bath, is filtered
Liquid C；
S6 UF membrane: being fitted into row centrifugation in centrifuge tube for liquor C, when pressure is 0.2MPa, carry out UF membrane with ultrafiltration membrane,
Residue is removed after centrifugation to obtain heart liquid；
S7 takes off albumen: adjusting the centrifugate pH to 3 of S6 with 6mol/L hydrogen chloride solution, is extracted with the chloroform of isometric ratio
It takes twice, supernatant is stayed to be filtered, then eluted with sodium chloride solution, it is more to obtain de- albumen for eluent concentration dialysis desalination
Sugar juice D；
S8 depigmentation: being added macroporous absorbent resin into the de- proteoglycan solution D that S7 is obtained, and mixes, shaken at room temperature 1h, takes out
Filter, obtains decoloration polysaccharide solution；
S9 dialysis: the polysaccharide solution that will decolourize reverse flowing water dialysis, last concentrated frozen it is dry apple peel polysaccharide.
The pineapple bran dry powder mesh number that step S1 is pre-processed is less than 60 mesh.
It is 30 minutes that the microwave ultrasonic wave instrument that step S3 is extracted, which is the time extracted, and extracted under temperature 50 C,.
The envelope-bulk to weight ratio of the distilled water and apple peel dry powder that are added in step S3 extraction is 500:1ml/g.
Step S6 filtrate is centrifuged under the speed of 4000r/min, time 30min.
The molecule interception of bag filter used in step S9 dialysis is 8kDa.
Centrifugate is obtained in step S6 UF membrane needs 5 DEG C of placement 2h.
Embodiment two:
A kind of extracting method of apple peel polysaccharide, comprising the following steps:
S1 pretreatment: powder will be beaten after the apple peel of screening is freeze-dried, obtains apple peel dry powder；
S2 degreasing: apple peel dry powder obtained by step S1 is placed in container, be added 45 DEG C of boiling range petroleum ether refluxing extraction 1~
3 times, filter residue is collected, it is spare after natural drying；
S3 is extracted: distilled water is added in the filter residue that dries that step is obtained, and is then handled to obtain extracting solution B with microwave ultrasonic wave instrument；
S4 filtering: extracting solution B is filtered by vacuum, and filtrate A is obtained；
S5 enzymatic hydrolysis: cellulase and plant rennet is added in filtrate A, digests 60min under PH5.0 and 50 DEG C of water-bath, is filtered
Liquid C；
S6 UF membrane: being fitted into row centrifugation in centrifuge tube for liquor C, when pressure is 0.4MPa, carry out UF membrane with ultrafiltration membrane,
Residue is removed after centrifugation to obtain heart liquid；
S7 takes off albumen: adjusting the centrifugate pH to 3 of S6 with 6mol/L hydrogen chloride solution, is extracted with the chloroform of isometric ratio
It takes twice, supernatant is stayed to be filtered, then eluted with sodium chloride solution, it is more to obtain de- albumen for eluent concentration dialysis desalination
Sugar juice D；
S8 depigmentation: being added macroporous absorbent resin into the de- proteoglycan solution D that S7 is obtained, and mixes, shaken at room temperature 2h, takes out
Filter, obtains decoloration polysaccharide solution；
S9 dialysis: the polysaccharide solution that will decolourize reverse flowing water dialysis, last concentrated frozen it is dry apple peel polysaccharide.
The pineapple bran dry powder mesh number that step S1 is pre-processed is less than 60 mesh.
It is 30 minutes that the microwave ultrasonic wave instrument that step S3 is extracted, which is the time extracted, and extracted at 75 DEG C of temperature,.
The envelope-bulk to weight ratio of the distilled water and apple peel dry powder that are added in step S3 extraction is 500:1ml/g.
Step S6 filtrate is centrifuged under the speed of 4000r/min, time 30min.
The molecule interception of bag filter used in step S9 dialysis is 11kDa.
Centrifugate is obtained in step S6 UF membrane needs 6 DEG C of placement 2h.
Embodiment three
A kind of extracting method of apple peel polysaccharide, comprising the following steps:
S1 pretreatment: powder will be beaten after the apple peel of screening is freeze-dried, obtains apple peel dry powder；
S2 degreasing: apple peel dry powder obtained by step S1 is placed in container, and 60 DEG C of boiling range of petroleum ether refluxing extraction 3 is added
It is secondary, filter residue is collected, it is spare after natural drying；
S3 is extracted: distilled water is added in the filter residue that dries that step is obtained, and is then handled to obtain extracting solution B with microwave ultrasonic wave instrument；
S4 filtering: extracting solution B is filtered by vacuum, and filtrate A is obtained；
S5 enzymatic hydrolysis: cellulase and plant rennet is added in filtrate A, digests 30min under PH5.0 and 40 DEG C of water-bath, is filtered
Liquid C；
S6 UF membrane: being fitted into row centrifugation in centrifuge tube for liquor C, when pressure is 0.6MPa, carry out UF membrane with ultrafiltration membrane,
Residue is removed after centrifugation to obtain heart liquid；
S7 takes off albumen: adjusting the centrifugate pH to 3 of S6 with 6mol/L hydrogen chloride solution, is extracted with the chloroform of isometric ratio
It takes twice, supernatant is stayed to be filtered, then eluted with sodium chloride solution, it is more to obtain de- albumen for eluent concentration dialysis desalination
Sugar juice D；
S8 depigmentation: being added macroporous absorbent resin into the de- proteoglycan solution D that S7 is obtained, and mixes, shaken at room temperature 3h, takes out
Filter, obtains decoloration polysaccharide solution；
S9 dialysis: the polysaccharide solution that will decolourize reverse flowing water dialysis, last concentrated frozen it is dry apple peel polysaccharide.
The pineapple bran dry powder mesh number that step S1 is pre-processed is less than 60 mesh.
It is 30 minutes that the microwave ultrasonic wave instrument that step S3 is extracted, which is the time extracted, and extracted at 90 DEG C of temperature,.
The envelope-bulk to weight ratio of the distilled water and apple peel dry powder that are added in step S3 extraction is 500:1ml/g.
Step S6 filtrate is centrifuged under the speed of 4000r/min, time 30min.
The molecule interception of bag filter used in step S9 dialysis is 14kDa.
Centrifugate is obtained in step S6 UF membrane needs 8 DEG C of placement 2h.
Comparative example 1
Compared with Example 3, enzymolysis step is free of.
Comparative example 2
Compared with Example 3, zytase is used in enzymolysis step, other are same as Example 3.
Comparative example 3
Compared with Example 3, pectase is used in enzymolysis step, other are same as Example 3.
The polysaccharide yield obtained to the various enzymes for being separately added into equivalent in enzymolysis step is tested, and the conclusion obtained is such as
Under:
This it appears that cellulase and plant rennet, which are added to have enzymatic hydrolysis simultaneously, greatly facilitates work from attached drawing 1
With improving polysaccharide yield.
It is obvious to a person skilled in the art that invention is not limited to the details of the above exemplary embodiments, Er Qie
In the case where without departing substantially from spirit or essential attributes of the invention, the present invention can be realized in other specific forms.Therefore, no matter
From the point of view of which point, the present embodiments are to be considered as illustrative and not restrictive, and the scope of the present invention is by appended power
Benefit requires rather than above description limits, it is intended that all by what is fallen within the meaning and scope of the equivalent elements of the claims
Variation is included within the present invention.Any reference signs in the claims should not be construed as limiting the involved claims.
In addition, it should be understood that although this specification is described in terms of embodiments, but not each embodiment is only wrapped
Containing an independent technical solution, this description of the specification is merely for the sake of clarity, and those skilled in the art should
It considers the specification as a whole, the technical solutions in the various embodiments may also be suitably combined, forms those skilled in the art
The other embodiments being understood that.
Claims (7)
1. a kind of extracting method of apple peel polysaccharide, which comprises the following steps:
S1 pretreatment: powder will be beaten after the apple peel of screening is freeze-dried, obtains apple peel dry powder；
S2 degreasing: apple peel dry powder obtained by step S1 is placed in container, and 30~60 DEG C of boiling range of petroleum ether reflux is added and mentions
It takes 1~3 time, collects filter residue, it is spare after natural drying；
S3 is extracted: distilled water is added in the filter residue that dries that step is obtained, and is then handled to obtain extracting solution B with microwave ultrasonic wave instrument；
S4 filtering: extracting solution B is filtered by vacuum, and filtrate A is obtained；
S5 enzymatic hydrolysis: cellulase and plant rennet is added in filtrate A, digests 30-90min at PH5.0 and 40-70 DEG C of water-bath,
Obtain liquor C；
S6 UF membrane: liquor C is fitted into row centrifugation in centrifuge tube, when pressure is 0.2~0.6MPa, carries out film with ultrafiltration membrane
Separation, removes residue after centrifugation to obtain heart liquid；
S7 takes off albumen: adjusting the centrifugate pH to 3 of S6 with 6mol/L hydrogen chloride solution, is extracted with the chloroform of isometric ratio
It takes twice, supernatant is stayed to be filtered, then eluted with sodium chloride solution, it is more to obtain de- albumen for eluent concentration dialysis desalination
Sugar juice D；
S8 depigmentation: being added macroporous absorbent resin into the de- proteoglycan solution D that S7 is obtained, and mixes, 1~3h of shaken at room temperature,
It filters, obtains decoloration polysaccharide solution；
S9 dialysis: the polysaccharide solution that will decolourize reverse flowing water dialysis, last concentrated frozen it is dry apple peel polysaccharide.
2. the extracting method of apple peel polysaccharide according to claim 1, which is characterized in that the step S1 is pre-processed
The pineapple bran dry powder mesh number arrived is less than 60 mesh.
3. the extracting method of apple peel polysaccharide according to claim 1, which is characterized in that the step S3 is extracted micro-
It is 30 minutes that wave ultrasonoscope, which is the time extracted, and extracted at 50~90 DEG C of temperature,.
4. the extracting method of apple peel polysaccharide according to claim 1, which is characterized in that add in the step S3 extraction
The envelope-bulk to weight ratio of the distilled water and apple peel dry powder that enter is 500:1ml/g.
5. the extracting method of apple peel polysaccharide according to claim 1, which is characterized in that the step S6 filtrate exists
It is centrifuged under the speed of 4000r/min, time 30min.
6. the extracting method of apple peel polysaccharide according to claim 1, which is characterized in that institute in the step S9 dialysis
The molecule interception of bag filter is 8-14kDa.
7. the extracting method of apple peel polysaccharide according to claim 1, which is characterized in that in the step S6 UF membrane
Obtaining centrifugate needs 5-8 DEG C of placement 2h.
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| CN201810748785.9A CN109134675A (en) | 2018-07-10 | 2018-07-10 | A kind of extracting method of apple peel polysaccharide |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2022013161A1 (en) * | 2020-07-16 | 2022-01-20 | Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. | Process for obtaining glycan extracts from apple pomace, process for producing functionalized glycans, and functionalized glycan that can be produced by the process and its use |
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| CN101348531A (en) * | 2008-08-26 | 2009-01-21 | 中国农业大学 | Pectin rapid classification method |
| CN102161713A (en) * | 2011-03-24 | 2011-08-24 | 铜川市耀州爱普乐生物科技有限公司 | Method for continuously preparing high-purity pectin by using enzymolysis-ultrafiltration concentration-spray drying process |
| CN106496355A (en) * | 2016-11-07 | 2017-03-15 | 涟源康麓生物科技有限公司 | A kind of preparation method of pectin |
| KR20180036154A (en) * | 2016-09-30 | 2018-04-09 | 세종대학교산학협력단 | Method for production of pectin from apple peel by organic acid with high yield |
| CN108003251A (en) * | 2017-11-24 | 2018-05-08 | 中国热带农业科学院南亚热带作物研究所 | A kind of extracting method of pineapple bran polysaccharide |
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2018
- 2018-07-10 CN CN201810748785.9A patent/CN109134675A/en not_active Withdrawn
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101348531A (en) * | 2008-08-26 | 2009-01-21 | 中国农业大学 | Pectin rapid classification method |
| CN102161713A (en) * | 2011-03-24 | 2011-08-24 | 铜川市耀州爱普乐生物科技有限公司 | Method for continuously preparing high-purity pectin by using enzymolysis-ultrafiltration concentration-spray drying process |
| KR20180036154A (en) * | 2016-09-30 | 2018-04-09 | 세종대학교산학협력단 | Method for production of pectin from apple peel by organic acid with high yield |
| CN106496355A (en) * | 2016-11-07 | 2017-03-15 | 涟源康麓生物科技有限公司 | A kind of preparation method of pectin |
| CN108003251A (en) * | 2017-11-24 | 2018-05-08 | 中国热带农业科学院南亚热带作物研究所 | A kind of extracting method of pineapple bran polysaccharide |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2022013161A1 (en) * | 2020-07-16 | 2022-01-20 | Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. | Process for obtaining glycan extracts from apple pomace, process for producing functionalized glycans, and functionalized glycan that can be produced by the process and its use |
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