CN109105366A - A method of utilizing gossypol degradation Sweet Potato Feathery Mottle Virus capsid protein - Google Patents
A method of utilizing gossypol degradation Sweet Potato Feathery Mottle Virus capsid protein Download PDFInfo
- Publication number
- CN109105366A CN109105366A CN201811084268.2A CN201811084268A CN109105366A CN 109105366 A CN109105366 A CN 109105366A CN 201811084268 A CN201811084268 A CN 201811084268A CN 109105366 A CN109105366 A CN 109105366A
- Authority
- CN
- China
- Prior art keywords
- sweet potato
- mottle virus
- gossypol
- capsid protein
- feathery mottle
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N35/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical
- A01N35/04—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical containing aldehyde or keto groups, or thio analogues thereof, directly attached to an aromatic ring system, e.g. acetophenone; Derivatives thereof, e.g. acetals
Abstract
The invention discloses a kind of methods using gossypol degradation Sweet Potato Feathery Mottle Virus capsid protein.Aiming at the problem that treating the virosis purpose there has been no degradation Sweet Potato Feathery Mottle Virus capsid protein to reach, to influence the stability of virion the treatment of virosis is advantageously implemented using gossypol viral capsid proteins of directly degrading.Compared with the upper traditional Sweet Potato Feathery Mottle Virus disease prevention and control of production utilize Resistant kind, the chemically or physically means such as means control mediator, viral antagonist control virus, this technology is directly degraded Sweet Potato Feathery Mottle Virus capsid protein using gossypol, so as to directly treat virosis, pest controlling mode is novel, safety is also higher, will be with certain application prospect.
Description
One, technical field
The invention belongs to pest control technical fields, and in particular to a kind of to utilize gossypol degradation Sweet Potato Feathery Mottle Virus
The method of capsid protein.
Two, technical background
In worldwide, the viroses of plant are the important threats that Crop securify efficiently produces, only in China, every year
The loss as caused by crops virus infection is as high as tens dollars or so.Currently still it is with prevention to the prevention and treatment of virosis
It is main, disease-resistant crop and chemical prevention etc. are relied primarily on, safely and effectively virosis remedy measures are still lacked in production.It effectively to control
The viroses of plant are treated, it is the most key to the directly destruction removing of virus, however the research report that current related plant virus is removed
It is also less.Existing antiviral substance main function target is to inhibit virus infection, inhibit virus multiplication and transhipment, inhibit virus
The expression of symptom and induced resistance etc..The capsid of viral capsid proteins composition is virus genomic important protective barrier,
It is most important for the stability of virion.But also much not using viral capsid proteins as the action target of antiviral substance
It is realized and develops, the active principle effectively destroyed to viral capsid proteins is expected to successfully develop as ideal viral therapy
Agent.
It is the important channel that screening obtains antiviral drugs that Metabolites against Plant Virus Diseases matter is found from nature, with section
The raising that the development of skill and people recognize environmental protection, plant source disease-resistant cytotoxic activity substance by more and more Experts ' Attentions,
It has been found that plant source disease-resistant noxious material it is also more and more, but not yet discovery plant source disease-resistant noxious material can directly degrade virus
Capsid protein.Gossypol is a kind of double naphthalene aldehyde compounds of yellow polyphenol hydroxyl, be primarily present in the root of high mallow plant cotton, stem,
In leaf and seed, wherein content highest in Cotton Seed.It has been found that gossypol medically can be used as a kind of effective male contraceptive agent,
It also can antitumor, avian influenza virus etc., it was found that it is relevant with bt-cotton characteristic in agriculturally gossypol, also it is resistant to tomato yellowing
Leaf curl viral disease, southern black streak dwarf etc..Sweet Potato Feathery Mottle Virus disease is the destructive disease on sweet potato, 70 years 20th century
In generation, finds that major sweet potato growing area has generation in the world at present, and it is short that the sweet potato of virus infection shows as plant in Africa first
Change, the symptoms such as blade chlorisis, distortion, deformity seriously affect yield of sweet potato, and when repeating transmission can cause 70% or more production loss.
Sweet Potato Feathery Mottle Virus can also be propagated by root tuber, grafting and mechanical inoculation by insect mediator perishability.Whether gossypol
There is degradation to the capsid protein of Sweet Potato Feathery Mottle Virus? it yet there are no research report both at home and abroad.Present invention research gossypol
To the degrading activity of Sweet Potato Feathery Mottle Virus capsid protein, to obtain a kind of medicine of Sweet Potato Feathery Mottle Virus disease
Agent, the highly effective and safe prevention and control for the disease.
Three, summary of the invention
1, goal of the invention
A kind of ideal plant vims capsid protein biodegrading process is provided, it can safe disposal Sweet Potato Feathery Mottle Virus capsid
Albumen provides new method for the treatment of the virosis.
2, technical solution
The present invention is a kind of biodegrading process of plant vims capsid protein, it is characterised in that utilizes gossypol degradation sweet potato pinniform
Mottle virus capsid albumen.
3, beneficial effect
Method of the invention compared with prior art, generates following the utility model has the advantages that (1) and conventional anti-plant virus agent press down
Virus infection processed duplication is compared, and the present invention removes virus by direct degrading plant viral capsid proteins, prevention and control thinking it is new and
It is with strong points；(2) compared with chemical antiviral agent, the present invention using as the gossypol of Chinese medicine, can improving plant virus conscientiously
The non-polluted food base of disease is horizontal, effectively reduces environmental pollution.
Four, specific embodiment
The embodiment of the present invention is the method using indoor measurement.
Measuring method is as follows:
1, the prokaryotic expression of viral capsid proteins
Using the method for being based on PAS (PCR-based Accurate Synthesis), design overall length splices primer, synthesis
Sweet Potato Feathery Mottle Virus capsid protein gene is connected into carrier pCzn1；The recombinant plasmid pCzn1-Gene2 of acquisition is transferred to TOP10
Clone strain and Arctic-ExpressTM express bacterial strain.Using IPTG inducing expression destination protein, Ni column affinity purification is obtained
Destination protein.
2, gossypol degradation viral capsid proteins
98% gossypol acetate is dissolved in N ' dinethylformamide and is made into 106Mg/L mother liquor, it is sweet to add to 125mg/L
In potato pinniform mottle virus capsid albumen, make the final concentration of 100mg/L of gossypol acetate, with without gossypol but containing with
The protein liquid of equal amount N ' dinethylformamide compares in 100mg/L gossypol protein liquid, places 20min in 37 DEG C of water-baths,
Albumen coagulates amine electrophoresis poststaining through polyacrylamide after processing, takes pictures.
Implementation result:
The study found that 125mg/L Sweet Potato Feathery Mottle Virus capsid protein through 100mg/L gossypol processing after, compared with without cotton
The control of phenol processing is compared, and protein electrophoresis band obviously dies down, and shows gossypol acetate to Sweet Potato Feathery Mottle Virus capsid egg
It is white to have apparent degradation (Fig. 1).
Detailed description of the invention:
Fig. 1 Sweet Potato Feathery Mottle Virus capsid protein is through gossypol treated SDS-PAGE electrophoresis.
Claims (1)
1. a kind of biodegrading process of plant vims capsid protein, it is characterised in that utilize gossypol degradation Sweet Potato Feathery Mottle Virus clothing
Glutelin.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811084268.2A CN109105366A (en) | 2018-09-11 | 2018-09-11 | A method of utilizing gossypol degradation Sweet Potato Feathery Mottle Virus capsid protein |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811084268.2A CN109105366A (en) | 2018-09-11 | 2018-09-11 | A method of utilizing gossypol degradation Sweet Potato Feathery Mottle Virus capsid protein |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109105366A true CN109105366A (en) | 2019-01-01 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811084268.2A Pending CN109105366A (en) | 2018-09-11 | 2018-09-11 | A method of utilizing gossypol degradation Sweet Potato Feathery Mottle Virus capsid protein |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1489904A (en) * | 2003-08-27 | 2004-04-21 | 吴云锋 | Polyhydroxy binaphthalene fatty acid preparation and preparing method thereof |
| CN103563897A (en) * | 2013-07-12 | 2014-02-12 | 江苏省农业科学院 | Method for reducing plant virus carrying rate in insect medium by utilizing gossypol |
| CN104910041A (en) * | 2014-03-12 | 2015-09-16 | 南开大学 | Aromatic amine schiff base derivative of gossypol and preparation method and plant-virus resisting application thereof |
| WO2015194993A1 (en) * | 2014-06-17 | 2015-12-23 | Limited Liability Company "Nearmedic Plus" | Gossypol polymer derivatives, process for preparing and pharmaceutical compositions thereof |
| CN107173384A (en) * | 2017-05-24 | 2017-09-19 | 江苏省农业科学院 | A kind of method that utilization gossypol acetate removes plant virus in tomato plant |
-
2018
- 2018-09-11 CN CN201811084268.2A patent/CN109105366A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1489904A (en) * | 2003-08-27 | 2004-04-21 | 吴云锋 | Polyhydroxy binaphthalene fatty acid preparation and preparing method thereof |
| CN103563897A (en) * | 2013-07-12 | 2014-02-12 | 江苏省农业科学院 | Method for reducing plant virus carrying rate in insect medium by utilizing gossypol |
| CN104910041A (en) * | 2014-03-12 | 2015-09-16 | 南开大学 | Aromatic amine schiff base derivative of gossypol and preparation method and plant-virus resisting application thereof |
| WO2015194993A1 (en) * | 2014-06-17 | 2015-12-23 | Limited Liability Company "Nearmedic Plus" | Gossypol polymer derivatives, process for preparing and pharmaceutical compositions thereof |
| CN107173384A (en) * | 2017-05-24 | 2017-09-19 | 江苏省农业科学院 | A kind of method that utilization gossypol acetate removes plant virus in tomato plant |
Non-Patent Citations (3)
| Title |
|---|
| LEI ZHAO ET AL: "Advances and prospects in biogenic substances against plant virus: A review", 《PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY 》 * |
| LEI ZHAO ET AL: "First Discovery of Acetone Extract from Cottonseed Oil Sludge as a Novel Antiviral Agent against Plant Viruses", 《PLOS ONE》 * |
| LING LI ET AL: "Design, Synthesis, and Biological Activities of Aromatic Gossypol Schiff Base Derivatives", 《J. AGRIC. FOOD CHEM》 * |
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| PB01 | Publication | ||
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| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20190101 |
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| WD01 | Invention patent application deemed withdrawn after publication |