CN109042652A - A kind of biodegrading process of sweet potato chlorotic stunt virus capsid protein - Google Patents
A kind of biodegrading process of sweet potato chlorotic stunt virus capsid protein Download PDFInfo
- Publication number
- CN109042652A CN109042652A CN201811084267.8A CN201811084267A CN109042652A CN 109042652 A CN109042652 A CN 109042652A CN 201811084267 A CN201811084267 A CN 201811084267A CN 109042652 A CN109042652 A CN 109042652A
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- CN
- China
- Prior art keywords
- stunt virus
- capsid protein
- sweet potato
- gossypol
- virus
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N35/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical
- A01N35/04—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical containing aldehyde or keto groups, or thio analogues thereof, directly attached to an aromatic ring system, e.g. acetophenone; Derivatives thereof, e.g. acetals
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- Life Sciences & Earth Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Plant Pathology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a kind of methods using gossypol degradation chlorisis stunt virus capsid protein.Aiming at the problem that in production there has been no using chlorisis stunt virus capsid protein as target treatment medicament, to influence the stability of virion the treatment of virosis is advantageously implemented using gossypol viral capsid proteins of directly degrading.Compared with the upper traditional sweet potato chlorotic stunt virus disease prevention and control of production utilize Resistant kind, the chemically or physically means such as means control mediator, viral antagonist control disease, this technology is directly degraded the capsid protein of sweet potato chlorotic stunt virus using gossypol, so as to directly treat virosis, pest controlling mode is novel, will be with certain application prospect.
Description
One, technical field
The invention belongs to pest control technical fields, and in particular to a kind of to utilize gossypol degradation sweet potato chlorotic stunt virus
The method of capsid protein.
Two, technical background
The viroses of plant seriously threaten the efficient production of Crop securify, lose 200 caused by annual in worldwide
Hundred million dollars or so.Prevention and treatment for virosis, currently still aims at prevention, and relies primarily on disease-resistant crop and chemical prevention etc., raw
Still lack safely and effectively virosis remedy measures in production.The viroses of plant are effectively treated, directly destroying for virus is removed
It is the most key, however the research report that current related plant virus is removed is also less.Existing viral antagonist is mainly for disease
Links, the emphasis such as poison infects, virus multiplication and transhipment inhibit the further proliferation of virus to extend, improve the disease resistance of plant.And
The existing still rare method of virus in plant or infection insect mediator how is just removed, effectively to remove virus, it is existing to destroy virus
Structure and composition composition is crucial.It is virus genomic important protective barrier by the capsid that viral capsid proteins form, it is right
It is most important in the stability of virion, such as it is destroyed, it will effectively remove virus.But viral capsid proteins are made
It is also fully developed far away for the action target of antiviral substance, and has the substance of degrading activity that will be expected to viral capsid proteins
It is ideal virus treatment agent that success, which is developed,.
It is the important channel that screening obtains antiviral drugs that Metabolites against Plant Virus Diseases matter is found from nature, with people
The understanding of environmental protection is stepped up, plant source disease-resistant cytotoxic activity substance is by more and more Experts ' Attentions, it has been found that
Plant source disease-resistant noxious material is also more and more, but not yet discovery plant source disease-resistant noxious material can directly degrade viral capsid egg
It is white.Gossypol is that one kind is largely present in cotton, stem, leaf and intraseminal polyphenol compound, wherein content highest in Cotton Seed.
It has been found that gossypol medically has wide purposes, a kind of effective male contraceptive agent can not only be used for, it also can antitumor, bird flu
Virus etc. is agriculturally then having found that gossypol is relevant with bt-cotton characteristic, is also being resistant to tomato yellow leaf curl virus disease, south
Black streak dwarf etc..Sweet potato chlorotic stunt virus disease is the commonly-occurring disease evil on sweet potato, is often occurred with the mixing of pinniform piebaldism viral disease,
The sweet potato of virus infection shows as plant dwarfing, and the symptoms such as the dwarfing of blade chlorisis, distortion, deformity cause seriously yield of sweet potato
It influences.Does gossypol have degradation to the capsid protein of sweet potato chlorotic stunt virus? it yet there are no research report both at home and abroad.This
Invention research gossypol is to the degrading activity of sweet potato chlorotic stunt virus capsid protein, to obtain a kind of sweet potato chlorotic stunt virus
The healing potion of disease, the safe and efficient prevention and control for the disease.
Three, summary of the invention
1, goal of the invention
A kind of ideal plant vims capsid protein biodegrading process is provided, sweet potato chlorotic stunt virus can be safely and effectively decomposed
Capsid protein provides new method for the treatment of the virosis.
2, technical solution
The present invention is a kind of biodegrading process of plant vims capsid protein, it is characterised in that utilizes gossypol degradation sweet potato chlorisis
Stunt virus capsid protein.
3, beneficial effect
Method of the invention compared with prior art, generates following the utility model has the advantages that (1) and conventional anti-plant virus agent press down
Virus infection processed duplication is compared, and the present invention destroys virus by direct degrading plant viral capsid proteins, prevention and control thinking it is new and
It is with strong points；(2) compared with chemical antiviral agent, the present invention using as the gossypol of Chinese medicine, can improving plant virus conscientiously
The non-polluted food base of disease is horizontal, effectively reduces environmental pollution.
Four, specific embodiment
The embodiment of the present invention is the method using indoor measurement.
Measuring method is as follows:
1, the prokaryotic expression of viral capsid proteins
Using the method for being based on PAS (PCR-based Accurate Synthesis), design overall length splices primer, synthesis
Sweet potato chlorotic stunt virus capsid protein gene is connected into carrier pCzn1；The recombinant plasmid pCzn1-Gene2 of acquisition is transferred to TOP10
Clone strain and Arctic-ExpressTM express bacterial strain.Using IPTG inducing expression destination protein, Ni column affinity purification is obtained
Destination protein.
2, gossypol degradation viral capsid proteins
98% gossypol acetate is dissolved in N ' dinethylformamide and is made into 106Mg/L mother liquor, it is sweet to add to 100mg/L
In potato chlorisis stunt virus capsid protein, make the final concentration of 100mg/L of gossypol acetate, with without gossypol but containing with
The viral capsid proteins liquid of equal amount N ' dinethylformamide compares in 100mg/L gossypol protein liquid, puts in 37 DEG C of water-baths
20min is set, albumen coagulates amine electrophoresis poststaining through polyacrylamide after processing, takes pictures.
Implementation result:
The study found that 100mg/L sweet potato chlorotic stunt virus capsid protein is after the processing of 100mg/L gossypol, and without cotton
The control of phenol processing is compared, and protein electrophoresis band obviously dies down, and shows gossypol acetate to sweet potato chlorotic stunt virus capsid egg
It is white to have apparent degradation (Fig. 1).
Detailed description of the invention:
Fig. 1 sweet potato chlorotic stunt virus capsid protein is through gossypol treated SDS-PAGE electrophoresis.
Claims (1)
1. a kind of biodegrading process of plant vims capsid protein, it is characterised in that utilize gossypol degradation sweet potato chlorotic stunt virus clothing
Glutelin.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811084267.8A CN109042652A (en) | 2018-09-11 | 2018-09-11 | A kind of biodegrading process of sweet potato chlorotic stunt virus capsid protein |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811084267.8A CN109042652A (en) | 2018-09-11 | 2018-09-11 | A kind of biodegrading process of sweet potato chlorotic stunt virus capsid protein |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109042652A true CN109042652A (en) | 2018-12-21 |
Family
ID=64762773
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811084267.8A Pending CN109042652A (en) | 2018-09-11 | 2018-09-11 | A kind of biodegrading process of sweet potato chlorotic stunt virus capsid protein |
Country Status (1)
| Country | Link |
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| CN (1) | CN109042652A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110506754A (en) * | 2019-08-06 | 2019-11-29 | 江苏省农业科学院 | A method of utilizing disinsection fungal degradation tomato yellow leaf curl virus capsid protein |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1489904A (en) * | 2003-08-27 | 2004-04-21 | 吴云锋 | Polyhydroxy binaphthalene fatty acid preparation and preparing method thereof |
| CN104910041A (en) * | 2014-03-12 | 2015-09-16 | 南开大学 | Aromatic amine schiff base derivative of gossypol and preparation method and plant-virus resisting application thereof |
| CN105884634A (en) * | 2015-01-05 | 2016-08-24 | 南开大学 | Gossypol derivatives and preparation thereof, application of gossypol derivatives in pesticide and anti-cancer activity |
-
2018
- 2018-09-11 CN CN201811084267.8A patent/CN109042652A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1489904A (en) * | 2003-08-27 | 2004-04-21 | 吴云锋 | Polyhydroxy binaphthalene fatty acid preparation and preparing method thereof |
| CN104910041A (en) * | 2014-03-12 | 2015-09-16 | 南开大学 | Aromatic amine schiff base derivative of gossypol and preparation method and plant-virus resisting application thereof |
| CN105884634A (en) * | 2015-01-05 | 2016-08-24 | 南开大学 | Gossypol derivatives and preparation thereof, application of gossypol derivatives in pesticide and anti-cancer activity |
Non-Patent Citations (1)
| Title |
|---|
| ZHAO L ET AL.: ""First Discovery of Acetone Extract from Cottonseed Oil Sludge as a Novel Antiviral Agent against Plant Viruses"", 《PLOS ONE》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110506754A (en) * | 2019-08-06 | 2019-11-29 | 江苏省农业科学院 | A method of utilizing disinsection fungal degradation tomato yellow leaf curl virus capsid protein |
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| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
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| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20181221 |
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| WD01 | Invention patent application deemed withdrawn after publication |