CN109125303A - A kind of biodegrading process of rabbit hemorrhagic disease virus capsid protein - Google Patents
A kind of biodegrading process of rabbit hemorrhagic disease virus capsid protein Download PDFInfo
- Publication number
- CN109125303A CN109125303A CN201811084266.3A CN201811084266A CN109125303A CN 109125303 A CN109125303 A CN 109125303A CN 201811084266 A CN201811084266 A CN 201811084266A CN 109125303 A CN109125303 A CN 109125303A
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- Prior art keywords
- rabbit
- hemorrhagic disease
- capsid protein
- disease virus
- rabbit hemorrhagic
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/11—Aldehydes
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/12—Antivirals
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Pharmacology & Pharmacy (AREA)
- Veterinary Medicine (AREA)
- Chemical & Material Sciences (AREA)
- Public Health (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Organic Chemistry (AREA)
- Communicable Diseases (AREA)
- Virology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Oncology (AREA)
- Epidemiology (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Abstract
The invention discloses a kind of methods using gossypol degradation rabbit hemorrhagic disease virus capsid protein.For the upper status for reaching the treatment virosis purpose there has been no degradation rabbit hemorrhagic disease virus capsid protein of production, to influence the stability of virion the treatment of virosis is advantageously implemented using gossypol viral capsid proteins of directly degrading.Compared with the upper traditional rabbit hemorrhagic disease prevention and control of production mainly pass through the progress virosis prevention such as antibiotic, vaccine, this technology is directly degraded rabbit hemorrhagic disease virus capsid protein using gossypol, to be expected to directly treat virosis, the mode of action is novel, safety is also higher, and the sound development of rabbit industry is beneficial to after.
Description
One, technical field
The invention belongs to animal virosis Control Technology fields, and in particular to a kind of to utilize gossypol degradation rabbit hemorrhagic disease virus
The method of capsid protein.
Two, technical background
With the improvement of people ' s living standards and the diversification of dietary structure, the cultivation amount of rabbit are also increasing.With
The increasing of rabbit cultivation amount, the frequency of occurrences of rabbit is also higher and higher, brings significant damage, especially rabbit viral to rabbit keeping
The harm of haemorrhage is the most serious, caused by economic loss it is very big.Rabbit hemorrhagic disease is also known as rabbit hemorrhagic pneumonia, rabbit bleeding
Disease or rabbit pest are a kind of rabbit infectious diseases as caused by rabbit hemorrhagic disease virus, and morbidity is anxious, infectiousness is strong, the high (Chang Da of the death rate
70% or more).There is no good drug to treat the disease at present, antibiotic and other drugs can only play pre- disease prevention and its
The effect of his complication.Vaccine inoculation is current common preventing control method, wherein traditional rabbit pestilence seedling is Attenuated vaccine,
Production needs to be injected sensitive rabbit with Rabbit pest virus, and preparation process needs carry out under the stringent bio-safety precautionary measures,
Otherwise it is be easy to cause virulent diffusion, additionally needs and uses a large amount of sensitive rabbit, higher cost affects production of vaccine use.Base
Because engineered vaccine is to produce vaccine by cell, it is easier to realize large-scale production compared with Attenuated vaccine, also quality easy to control,
Biological safety is good, while protective rate having the same, will there is broader prospect.But vaccine is mainly the pre- of progress virosis
It is anti-, and can not be treated to rabbit hemorrhagic disease virus rabbit has been infected.It is very heavy that the effective therapeutic agent of rabbit hemorrhagic disease is excavated thus
It wants, and effectively to treat virosis, the most key, the current research report in relation to virus sweep is removed to directly destroying for virus
It is also less.The capsid of viral capsid proteins composition is virus genomic important protective barrier, for the stability of virion
It is most important.But it is not also much realized and develops using viral capsid proteins as the action target of antiviral substance, to virus
The substance that capsid protein is effectively destroyed is expected to successfully develop as ideal virus treatment agent.
Being found from nature to the natural active matter that viral capsid proteins have destruction is that screening obtains virus and controls
Treat the important channel of drug.Plant is the important sources of natural antiviral activity substance, it has been found that many botanical components have
Antiphytoviral, animal virus and human virus, but they are found the direct degradation of viral capsid proteins not yet.
Gossypol is the double naphthalene aldehyde compounds of the root for being largely present in cotton, stem, leaf and a kind of intraseminal yellow polyphenol hydroxyl, in medicine
On be widely used as male contraceptive pill, it has been found that its can antitumor, avian influenza virus etc., be resistant to tomato yellow in agriculturally gossypol
Change leaf curl viral disease, southern black streak dwarf etc..Does gossypol have degradation to the capsid protein of rabbit hemorrhagic disease virus? state
It is inside and outside to yet there are no research report.Present invention research gossypol is to the degrading activity of rabbit hemorrhagic disease virus capsid protein, to obtain one
The healing potion of kind rabbit hemorrhagic disease treats the disease for highly effective and safe, ensures the sound development of rabbit industry.
Three, summary of the invention
1, goal of the invention
A kind of ideal animal virus capsid protein biodegrading process is provided, it can safe disposal rabbit hemorrhagic disease virus capsid egg
It is white, new method is provided for the treatment of the virosis.
2, technical solution
The present invention is a kind of biodegrading process of animal virus capsid protein, it is characterised in that utilizes gossypol degradation rabbit haemorrhagic disease
Viral capsid proteins.
3, beneficial effect
Method of the invention compared with prior art, generates following the utility model has the advantages that the animal virosis of (1) and routine is with pre-
It is compared based on anti-, the present invention removes virus by directly degrading animal virus capsid protein, and prevention and control thinking is new and with strong points；
(2) present invention can improve the nothing of animal virosis using having extensive source and taking the gossypol of Chinese medicine as the mankind conscientiously
Public hazards controlled level.
Four, specific embodiment
The embodiment of the present invention is the method using indoor measurement.
Measuring method is as follows:
1, the prokaryotic expression of viral capsid proteins
Using the method for being based on PAS (PCR-based Accurate Synthesis), design overall length splices primer, synthesis
Capsid Gene of Chinese Isolate of Rabbit Hemorrhagic Disease Virus is connected into carrier pCzn1；The recombinant plasmid pCzn1- capsid protein gene of acquisition is transferred to
TOP10 clone strain and Arctic-ExpressTM express bacterial strain.Use IPTG inducing expression destination protein, Ni column affinity purification
Obtain destination protein.
2, gossypol degradation viral capsid proteins
98% gossypol acetate is dissolved in N ' dinethylformamide and is made into 106Mg/L mother liquor adds to 225mg/L rabbit
In haemorrhagic virus capsid protein, make the final concentration of 100mg/L of gossypol acetate, with without gossypol but contain and 100mg/L cotton
The protein liquid of equal amount N ' dinethylformamide compares in phenol protein liquid, and 20min, egg after processing are placed in 37 DEG C of water-baths
It is white to coagulate amine electrophoresis poststaining through polyacrylamide, it takes pictures.
Implementation result:
The study found that 225mg/L rabbit hemorrhagic disease virus capsid protein through 100mg/L gossypol processing after, at without gossypol
Ratio is managed, protein electrophoresis band obviously dies down, and shows that gossypol acetate has degradation (Fig. 1) to viral capsid proteins.
Detailed description of the invention:
Fig. 1 rabbit hemorrhagic disease virus capsid protein is through gossypol treated SDS-PAGE electrophoresis.
Claims (1)
1. a kind of biodegrading process of animal virus capsid protein, it is characterised in that utilize gossypol degradation rabbit hemorrhagic disease virus capsid egg
White method.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811084266.3A CN109125303A (en) | 2018-09-11 | 2018-09-11 | A kind of biodegrading process of rabbit hemorrhagic disease virus capsid protein |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811084266.3A CN109125303A (en) | 2018-09-11 | 2018-09-11 | A kind of biodegrading process of rabbit hemorrhagic disease virus capsid protein |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109125303A true CN109125303A (en) | 2019-01-04 |
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ID=64814660
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811084266.3A Pending CN109125303A (en) | 2018-09-11 | 2018-09-11 | A kind of biodegrading process of rabbit hemorrhagic disease virus capsid protein |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109125303A (en) |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2011146527A2 (en) * | 2010-05-17 | 2011-11-24 | Zirus, Inc. | Mammalian genes involved in infection |
| CN105708863A (en) * | 2016-03-21 | 2016-06-29 | 江南大学 | Composition capable of achieving sun protection and preventing infection of mosquito-borne viruses such as Zika virus |
-
2018
- 2018-09-11 CN CN201811084266.3A patent/CN109125303A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2011146527A2 (en) * | 2010-05-17 | 2011-11-24 | Zirus, Inc. | Mammalian genes involved in infection |
| CN105708863A (en) * | 2016-03-21 | 2016-06-29 | 江南大学 | Composition capable of achieving sun protection and preventing infection of mosquito-borne viruses such as Zika virus |
Non-Patent Citations (1)
| Title |
|---|
| TIONE BURANDA ET AL.: "A High-Throughput Flow Cytometry Screen Identifies Molecules That Inhibit Hantavirus Cell Entry", 《SLAS DISCOVERY》 * |
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| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20190104 |
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| WD01 | Invention patent application deemed withdrawn after publication |