CN109082416A

CN109082416A - 具有除草剂抗性的ACCase突变型基因和蛋白及其应用

Info

Publication number: CN109082416A
Application number: CN201810959406.0A
Authority: CN
Inventors: 张保龙; 王金彦; 凌溪铁; 郭冬姝
Original assignee: Jiangsu Academy of Agricultural Sciences
Current assignee: Jiangsu Academy of Agricultural Sciences
Priority date: 2018-08-22
Filing date: 2018-08-22
Publication date: 2018-12-25
Anticipated expiration: 2038-08-22
Also published as: CN109082416B

Abstract

本发明公开了一种水稻ACCase突变型蛋白，所述ACCase突变型蛋白的氨基酸序列存在以下任意一种或几种突变：其对应于野生型水稻ACCase的氨基酸序列的第1796位、第1797位、第1835位、第1875位、第2010位、第2050位、第2060位、第2070位和第2106位发生突变。本发明还公开了一种核酸或基因，其编码所述的蛋白。本发明通过转基因方法将突变型基因在除草剂敏感水稻中表达，含有该突变蛋白的植物可以抗（耐）乙酰辅酶A羧化酶类除草剂。含有本发明的水稻ACCase蛋白的水稻三叶期幼苗在施用3mL喹禾灵/L水（2倍推荐使用浓度）后，植株仍然正常生长发育和结实。

Description

具有除草剂抗性的ACCase突变型基因和蛋白及其应用

技术领域

本发明属于植物蛋白和植物抗除草剂领域，涉及具有除草剂抗性的ACCase突变型基因、蛋白及其应用；具体而言，本发明涉及水稻的乙酰辅酶A羧化酶(ACCase)突变基因及其编码的突变ACCase蛋白，该突变蛋白能赋予植物尤其水稻抗乙酰辅酶A羧化酶抑制剂类除草剂的特性。本发明公开了该突变蛋白的核苷酸序列和氨基酸序列，以及它们在植物抗除草剂领域中的应用。

背景技术

农田杂草是导致农作物减产的最主要的原因之一。与传统依靠栽培措施、人工除草和机械除草等方法相比，化学除草剂的使用是人们公认的农田除草技术中最可靠且最经济的手段。自2,4-D于20世纪40年代开始使用以来，除草剂工业已有60多年的发展历史，迄今已成功开发了一大批选择性除草剂。其中乙酰辅酶A羧化酶(Acetyl-CoA Carboxylase，简称ACCase)是化学除草剂的一个重要靶标。ACCase酶存在于大多数的生物体中，主要存在于细菌、酵母、真菌、植物、动物和人类中。目前已经确定在植物体内有两种同工型的ACCase，主要位于质体和细胞溶胶中，它们的功能区域在结构上有很大的差别。大多数植物质体中的ACCase有4个亚基：一个是生物素羧基载体蛋白亚基(biotincarboxylcarrier-protein，BCCP)，一个是生物素羧化酶亚基(biotincarboxylase，BC)，以及羧基转移酶(carboxyl transferase，CT)的两个亚基CTα和CTβ，二者之间以共价键相连。大多数植物细胞溶胶中的ACCase与酵母、大鼠和人的ACCase属于同一类型，它的一条多肽链包含原核型ACCase所有的4个亚基，排列顺序是BC、BCCP、CTβ、CTα，形成了3个功能域(CTβ和CTα算一个功能域)。除草剂对ACCase的抑制主要是通过抑制羧基转移酶(CT)，造成酰基脂类生物合成受到抑制，影响细胞膜渗透性，造成代谢产物渗漏，致使植物死亡，从而达到防除禾本科杂草的目的。

ACCase是一种以生物素为辅基的多功能酶。它利用ATP供给的能量催化由乙酰辅酶A生成丙二酰-CoA的羧基化反应，该反应是脂肪酸全合成的第一个关键步骤，也是限速步骤，这步由ACCase催化的反应实际上是由3个亚基组成的两步反应。第一步，首先由ATP提供能量给重碳酸，产生活化的羧基(羧酸、磷酸复合物)，然后在BC作用下将羧基连接到BCCP中生物素的脲环N1上，形成羧化的生物素羧基载体蛋白，该过程中由Mg²⁺或者Mn²⁺等的参与，碳酸氢盐为羧基供体。第二步，在CT作用下，将生物素脲环N1上的羧基转移至乙酰-CoA的甲基，产生丙二酰-CoA，该过程不需要其他的辅助能量。合成的丙二酰-CoA参与脂肪酸从头合成等生物过程。

对于ACCase类抑制剂的研究始于20世纪70年代，ACCase类除草剂包括芳氧苯氧丙酸酯类(Aryloxyphenoxypropanoates，APP)、芳氧苯基环己二酮类(Aryloxyphenylcy-clohexanedione，APCHD)以及三酮类环己二酮(Cyclict riketones,CTR)。由于其选择性高，能够苗后防除一年或多年生禾本科杂草，且在植物体内传导，具有高效、低毒、施用期长、对后茬作物安全等优点，此类除草剂迅速发展、品种不断增多、使用面积迅速扩大，已成为世界第三类重要除草剂。

但是随着长期和大面积的使用，杂草很快对这两种除草剂产生了抗性，目前，已在26个国家发现了高达35种杂草对这两类抑制剂产生了抗性。

遗憾的是，ACCase抑制剂类除草剂的作用机理尚未确定，很难提前预测ACCase蛋白其它氨基酸位点的突变是否会产生除草剂抗性，只能依赖于科研人员长期、艰苦的实践探索，并凭借一些运气才可能发现ACCase蛋白新的除草剂抗性位点。

发明内容

技术问题：本发明所要解决的技术问题是提供一种可以赋予植物抗除草剂抗性的突变型蛋白。进一步地，本发明所要解决的技术问题是提供一种可以赋予植物抗(耐)乙酰辅酶A羧化酶类除草剂抗性的水稻ACCase突变型蛋白。

进一步地，本发明所要解决的技术问题是提供一种可以赋予植物抗除草剂抗性的突变型基因。进一步地，本发明所要解决的技术问题是提供一种可以赋予植物抗(耐)乙酰辅酶A羧化酶类除草剂抗性的水稻ACCase突变型基因。

为此，本发明提供了一种编码水稻ACCase突变型蛋白的核酸或基因。

在一种实施方式中，本发明提供了一种水稻ACCase突变型蛋白，所述ACCase突变型蛋白的氨基酸序列存在以下任意一种或多种突变：其对应于水稻ACCase的氨基酸序列的第1796位、第1797位、第1835位、第1875位、第2010位、第2050位、第2060位、第2070位、第2106位发生突变。

作为优选，本发明的水稻ACCase的氨基酸序列第1796位氨基酸由丙氨酸突变为甘氨酸、第1797位氨基酸由丙氨酸突变为脯氨酸、第1835位氨基酸由谷氨酰胺变为脯氨酸、第1875位氨基酸由缬氨酸变为苯丙氨酸、第2010位氨基酸由色氨酸变为甘氨酸、第2010位氨基酸由色氨酸变为半胱氨酸、第2050位氨基酸由谷氨酸变为甘氨酸、第2060位氨基酸由缬氨酸变为亮氨酸、第2070位氨基酸由丙氨酸变为缬氨酸、第2106位氨基酸由赖氨酸变为谷氨酸。

优选地，本发明的水稻ACCase的氨基酸序列第1796位氨基酸为除了丙氨酸之外的其它氨基酸、第1797位氨基酸为除了丙氨酸之外的其它氨基酸、第1835位氨基酸为除了谷氨酰胺之外的其它氨基酸、第1875位氨基酸为除了缬氨酸之外的氨基酸、第2010位氨基酸为除了色氨酸之外的其它氨基酸、第2010位氨基酸为除了色氨酸之外的其它氨基酸、第2050位氨基酸为除了谷氨酸之外的其它氨基酸、第2060位氨基酸为除了缬氨酸之外的其它氨基酸、第2070位氨基酸为除了丙氨酸之外的其它氨基酸、第2106位氨基酸为除了赖氨酸之外的其它氨基酸。

优选地，本发明的ACCase突变型蛋白发生突变的氨基酸包括但不仅限于以下几种突变，在以下位点突变成其他氨基酸的情况也在本发明的保护范围之内，例如：第1796位氨基酸由丙氨酸突变为苯丙氨酸、丝氨酸、酪氨酸、半胱氨酸、脯氨酸、苏氨酸、异亮氨酸、天冬氨酸、精氨酸、谷氨酰胺、色氨酸、赖氨酸、组氨酸、甲硫氨酸、亮氨酸、缬氨酸、谷氨酸、天冬氨酸；第1797位氨基酸由丙氨酸突变为苯丙氨酸、丝氨酸、酪氨酸、半胱氨酸、苏氨酸、异亮氨酸、天冬氨酸、甘氨酸、精氨酸、谷氨酰胺、色氨酸、赖氨酸、组氨酸、甲硫氨酸、亮氨酸、缬氨酸、谷氨酸、天冬氨酸；第1835位氨基酸由谷氨酰胺变为苯丙氨酸、丝氨酸、酪氨酸、半胱氨酸、苏氨酸、异亮氨酸、天冬氨酸、甘氨酸、精氨酸、谷氨酰胺、色氨酸、赖氨酸、组氨酸、甲硫氨酸、亮氨酸、缬氨酸、丙氨酸、天冬氨酸；第1875位氨基酸由缬氨酸突变为丝氨酸、酪氨酸、半胱氨酸、脯氨酸、苏氨酸、异亮氨酸、天冬氨酸、甘氨酸、精氨酸、谷氨酰胺、色氨酸、赖氨酸、组氨酸、甲硫氨酸、亮氨酸、丙氨酸、谷氨酸、天冬氨酸；第2010位氨基酸由色氨酸变为苯丙氨酸、丝氨酸、酪氨酸、脯氨酸、苏氨酸、异亮氨酸、天冬氨酸、精氨酸、谷氨酰胺、赖氨酸、组氨酸、甲硫氨酸、亮氨酸、缬氨酸、丙氨酸、谷氨酸、天冬氨酸；第2050位氨基酸由谷氨酸变为苯丙氨酸、丝氨酸、酪氨酸、半胱氨酸、脯氨酸、苏氨酸、异亮氨酸、天冬氨酸、精氨酸、谷氨酰胺、色氨酸、赖氨酸、组氨酸、甲硫氨酸、亮氨酸、缬氨酸、丙氨酸、天冬氨酸；第2060位氨基酸由缬氨酸变为苯丙氨酸、丝氨酸、酪氨酸、半胱氨酸、脯氨酸、苏氨酸、异亮氨酸、天冬氨酸、甘氨酸、精氨酸、谷氨酰胺、色氨酸、赖氨酸、组氨酸、甲硫氨酸、丙氨酸、谷氨酸、天冬氨酸；第2070位氨基酸由丙氨酸变为苯丙氨酸、丝氨酸、酪氨酸、半胱氨酸、脯氨酸、苏氨酸、异亮氨酸、天冬氨酸、甘氨酸、精氨酸、谷氨酰胺、色氨酸、赖氨酸、组氨酸、甲硫氨酸、亮氨酸、谷氨酸、天冬氨酸；第2106位氨基酸由赖氨酸变为苯丙氨酸、丝氨酸、酪氨酸、半胱氨酸、脯氨酸、苏氨酸、异亮氨酸、天冬氨酸、甘氨酸、精氨酸、谷氨酰胺、色氨酸、组氨酸、甲硫氨酸、亮氨酸、缬氨酸、丙氨酸、天冬氨酸。

本发明还提供一种水稻ACCase突变型蛋白，包括：

(a)其氨基酸序列选自由SEQ ID NO.2、SEQ ID NO.4、SEQ ID NO.6、SEQ ID NO.8、SEQ ID NO.10、SEQ ID NO.12、SEQ ID NO.14、SEQ ID NO.16、SEQ ID NO.18和SEQ IDNO.20组成的组中；或

(b)在(a)中的氨基酸序列经过取代和/或缺失和/或添加一个或多个氨基酸，且具有乙酰辅酶A羧化酶活性的由(a)衍生的蛋白质。

本发明还提供一种核酸或基因，其编码所述的蛋白。

本发明所述的核酸或基因，包括：

(i)编码所述的蛋白的核酸或基因；或

(ii)在严格条件下与(a)限定的核苷酸序列杂交且编码具有乙酰辅酶A羧化酶活性的蛋白质的核苷酸序列；或

(iii)其核苷酸序列选自由SEQ ID NO:1、SEQ ID NO:3、SEQ ID NO:5、SEQ ID NO:7、SEQ ID NO:9、SEQ ID NO:11、SEQ ID NO:13、SEQ ID NO:15、SEQ ID NO:17和SEQ ID NO:19组成的组中。

所述严格条件为：在0.1×SSC、0.1％SDS的溶液中，65℃洗膜15分钟的高度严谨的杂交条件。

本发明的ACCase突变型基因发生突变的核苷酸包括但不仅限于以下几种突变，在以下位点突变成其他核苷酸的情况也在本发明的保护范围之内，例如：A突变为T、C、G；或C突变为T、A、G；或T突变为A、C、G；或G突变为T、C、A等等均在本发明的保护范围之内。

进一步地，本发明的水稻ACCase突变型基因，所述水稻ACCase基因存在以下一种或多种突变：在水稻ACCase基因的第5387位核苷酸、在水稻ACCase基因的第5389位核苷酸、在水稻ACCase基因的第5504位核苷酸、在水稻ACCase基因的第5623位核苷酸、在水稻ACCase基因的第6028位核苷酸、在水稻ACCase基因的第6030位核苷酸、在水稻ACCase基因的第6149位核苷酸、在水稻ACCase基因的第6178位核苷酸、在水稻ACCase基因的第6209位核苷酸、在水稻ACCase基因的第6316位核苷酸发生突变。

作为优选，本发明的水稻ACCase突变型基因，所述水稻ACCase基因存在以下一种或多种突变：在水稻ACCase基因的第5387位核苷酸由C突变为G，在水稻ACCase基因的第5389位核苷酸由G突变为C，在水稻ACCase基因的第5504位核苷酸由A突变为C，在水稻ACCase基因的第5623位核苷酸由G突变为T，在水稻ACCase基因的第6028位核苷酸由T突变为G，在水稻ACCase基因的第6030位核苷酸由G突变为T，在水稻ACCase基因的第6149位核苷酸由A突变为C，在水稻ACCase基因的第6178位核苷酸由G突变为C，在水稻ACCase基因的第6209位核苷酸由C突变为T，在水稻ACCase基因的第6316位核苷酸由A突变为G。

本发明还提供一种表达盒、重组载体或细胞，其含有所述的核酸或基因。

本发明还提供所述的水稻ACCase突变型蛋白、核酸或基因，表达盒、重组载体或细胞在植物抗除草剂方面的应用。

其中，上述除草剂是喹禾灵、吡氟氯禾灵、烯草酮、吡氟禾草灵中的一种或多种。

本发明还提供获得具有除草剂抗性的植物的方法，包括如下步骤：

1)使植物包含所述核酸或基因；或

2)使植物表达所述水稻ACCase突变型蛋白。

进一步地，所述方法包括crispr基因编辑、转基因、杂交、回交或无性繁殖步骤。

本发明还提供一种鉴定植物的方法，其中所述植物是包含所述的核酸或基因的植物、表达所述的蛋白的植物或由所述的方法获得的植物，包括以下步骤：

1)测定所述植物是否包含所述的核酸或基因；或，

2)测定所述植物是否表达所述的蛋白。

本发明还提供一种除草剂的防护剂，该防护剂包括所述的水稻ACCase突变型蛋白。

本发明还提供一种控制杂草的方法，包括：对种植作物的大田施用有效剂量的除草剂，所述作物包含所述的核酸或基因或所述的表达盒、重组载体或细胞，所述除草剂为喹禾灵、吡氟氯禾灵、烯草酮、吡氟禾草灵中的一种或多种。

本发明还提供一种用于保护植物免受由除草剂引起的损伤的方法，包括：对种植作物的大田施用有效剂量的除草剂，所述作物包含所述的核酸或基因或将所述的表达盒、重组载体导入植物，导入后的植物产生除草剂抗性蛋白，所述除草剂为喹禾灵、吡氟氯禾灵、烯草酮、吡氟禾草灵中的一种或多种。

进一步地，本发明的野生型基因来源于水稻的嘉花1号。

进一步地，本发明的嘉花1号的野生型植株不具有喹禾灵除草剂抗性，本发明的含有ACCase突变位点的转基因嘉花1号植株能赋予水稻植物增加对乙酰辅酶A羧化酶类除草剂的耐受性。

其中，本发明的嘉花1号的突变型基因序列的编号及其对应的核苷酸和氨基酸序列分别是：嘉花1号的ACCase基因的第5387位核苷酸由C突变为G，1796位氨基酸由丙氨酸变为甘氨酸，其核苷酸序列如SEQ ID No:1所示，其氨基酸序列如SEQ ID No:2所示；

其中，嘉花1号的ACCase基因的第5389位核苷酸由G变为C，1797位氨基酸由丙氨酸变为脯氨酸，其核苷酸序列如SEQ ID No:3所示，其氨基酸序列如SEQ ID No:4所示；

其中，嘉花1号的ACCase基因的第5504位核苷酸由A变为C，1835位氨基酸由谷氨酰胺变为脯氨酸，其核苷酸序列如SEQ ID No:5所示，其氨基酸序列如SEQ ID No:6所示；

其中，嘉花1号的ACCase基因的第5623位核苷酸由G变为T，1875位氨基酸由缬氨酸变为苯丙氨酸，其核苷酸序列如SEQ ID No:7所示，其氨基酸序列如SEQ ID No:8所示；

其中，嘉花1号的ACCase基因的第6028位核苷酸由T变为G，2010位氨基酸由色氨酸变为甘氨酸，其核苷酸序列如SEQ ID No:9所示，其氨基酸序列如SEQ ID No:10所示；

其中，嘉花1号的ACCase基因的第6030位核苷酸由G变为T，2010位氨基酸由色氨酸变为半胱氨酸，其核苷酸序列如SEQ ID No:11所示，其氨基酸序列如SEQ ID No:12所示；

其中，嘉花1号的ACCase基因的第6149位核苷酸由A变为C，2050位氨基酸由谷氨酸变为甘氨酸，其核苷酸序列如SEQ ID No:13所示，其氨基酸序列如SEQ ID No:14所示；

其中，嘉花1号的ACCase基因的第6178位核苷酸由G变为C，2060位氨基酸由缬氨酸变为亮氨酸，其核苷酸序列如SEQ ID No:15所示，其氨基酸序列如SEQ ID No:16所示；

其中，嘉花1号的ACCase基因的第6209位核苷酸由C变为T，2070位氨基酸由丙氨酸变为缬氨酸，其核苷酸序列如SEQ ID No:17所示，其氨基酸序列如SEQ ID No:18所示；

其中，嘉花1号的ACCase基因的第6316位核苷酸由A变为G，2106位氨基酸由赖氨酸(Lys)变为谷氨酸，其核苷酸序列如SEQ ID No:19所示，其氨基酸序列如SEQ ID No:20所示；

其中，嘉花1号野生型ACCase基因核苷酸序列如SEQ ID No:21所示，其氨基酸序列如SEQ ID No:22所示。

本发明还提供了获得具有除草剂抗性的植物的方法。进一步地，本发明提供了用于增强植物、植物组织、植物细胞对于至少一种除草剂的耐受性的方法，所述除草剂干扰ACCase酶的活性。因此，本发明中除草剂抗性指的是抗(耐)乙酰辅酶A羧化酶类除草剂。

本发明所述的增强植物、植物组织或者植物细胞对除草剂的抗性方法可以通过转化或者杂交、自交和无性繁殖的方法实施，也可通过基因定点突变的方式获得，使改变的植株包括本发明的核苷酸序列SEQ ID No:1、SEQ ID No:3、SEQ ID No:5、SEQ ID No:7、SEQID No:9、SEQ ID No:11、SEQ ID No:13、SEQ ID No:15、SEQ ID No:17、SEQ ID No:19中的任意一种，所述的突变后的植株具有一种或者一种以上的上述突变后的核苷酸序列。

本发明还提供了耐受除草剂的水稻植物，其表达含有抗除草剂突变位点的ACCase基因，该基因序列不同于野生型水稻植物的ACCase基因序列，是ACCase基因含有抗除草剂突变位点的序列。发明人通过定向突变的方法获得了上述携带有突变位点的水稻ACCase突变序列，并将其转入到野生型水稻品种，发现携带有ACCase突变位点的转基因水稻可以抗ACCase类除草剂，而野生型水稻对ACCase类除草剂表现敏感。本发明还提供了所述核酸或基因及蛋白在植物育种中的应用，用于培育具有除草剂抗性的植物，尤其是农作物，还提供了这些蛋白及其编码基因在转基因或者非转基因如水稻等作物中的应用。

有益效果：相对于现有技术，本发明具备以下优点：

1)本发明首次通过同源比对的方式获得了具备除草剂抗性的水稻ACCase突变位点，并通过定点突变的方式扩增获得突变后的ACCase基因序列。

2)将ACCase突变序列转入到野生型水稻中，转基因水稻获得了ACCase酶类除草剂抗性。在田间喷施ACCase抑制剂类除草剂喹禾灵的实验结果表明，含有本发明的水稻ACCase突变蛋白的转基因水稻在施用2倍推荐使用浓度的乙酰辅酶A羧化酶抑制剂类除草剂后，植株仍然正常生长发育和结实，而野生型水稻幼苗在施用1倍推荐使用浓度的乙酰辅酶A羧化酶抑制剂类除草剂后，植株生长逐渐停止，叶片逐渐失绿、变浅黄色，植株不能拔节，最终整株死亡。

附图说明

图1示出了抗喹禾灵除草剂的含有ACCase突变位点的转基因水稻；

图2示出了PCR扩增10个含有水稻ACCase突变位点的ACCase基因全长结果图；第1泳道为Marker；Marker分子量从上到下依次为15kb、8kb、5kb、3kb、2kb、1.5kb、1kb、750bp、500bp，第2泳道为野生型嘉花1号的DNA，第3泳道为含有抗除草剂突变位点(ACCase-5387)的DNA，第4泳道为含有抗除草剂突变位点(ACCase-5389)的DNA，第5泳道为含有抗除草剂突变位点(ACCase-5504)的DNA，第6泳道为含有抗除草剂突变位点(ACCase-5623)的DNA，第7泳道为含有抗除草剂突变位点(ACCase-6028)的DNA，第8泳道为含有抗除草剂突变位点(ACCase-6030)的DNA，第9泳道为含有抗除草剂突变位点(ACCase-6149)的DNA，第10泳道为含有抗除草剂突变位点(ACCase-6178)的DNA，第11泳道为含有抗除草剂突变位点(ACCase-6209)的DNA，第12泳道为含有抗除草剂突变位点(ACCase-6316)的DNA。

具体实施方式

下面将结合实施例对本发明的实施方案进行详细描述，但是本领域技术人员将会理解，下列实施例仅用于说明本发明，而不应视为限定本发明的范围。实施例中未注明具体条件者，按照常规条件或制造商建议的条件进行。所用试剂或仪器未注明生产厂商者，均为可以通过市购获得的常规产品。

实施例1：水稻ACCase基因突变位点的确定

我们将大穗看麦娘(Alopecurus myosuroides)(黑草)质体的ACCase序列(Genbank AJ310767.1)作为参考序列，与水稻基因组进行同源比对，发现与水稻基因组的ACCase基因(XM_015783727.1)的同源性最高。根据专利(CN 102946714 B)中提到的黑草ACCase可以抗除草剂的氨基酸突变位点，分别是1785(丙氨酸突变为甘氨酸)、1786(丙氨酸突变为脯氨酸)、1824(谷氨酰胺变为脯氨酸)、1864(缬氨酸变为苯丙氨酸)、1999(色氨酸变为甘氨酸)、1999(色氨酸变为半胱氨酸)、2039(谷氨酸变为甘氨酸)、2049(缬氨酸变为亮氨酸)、2059(丙氨酸变为缬氨酸)、2095(赖氨酸变为谷氨酸)作为参考，通过同源比对的方法，确定在水稻中ACCase基因突变的氨基酸位点分别为1796(丙氨酸突变为甘氨酸)、1797(丙氨酸突变为脯氨酸)、1835(谷氨酰胺变为脯氨酸)、1875(缬氨酸变为苯丙氨酸)、2010(色氨酸变为甘氨酸)、2010(色氨酸变为半胱氨酸)、2050(谷氨酸变为甘氨酸)、2060(缬氨酸变为亮氨酸)、2070(丙氨酸变为缬氨酸)、2106(赖氨酸变为谷氨酸)。根据氨基酸密码子对照表，确定对应核苷酸位点分别为5387(C变为G)、5389(G变为C)、5504(A变为C)、5623(G变为T)、6028(T变为G)、6030(G变为T)、6149(A变为C)、6178(G变为C)、6209(C变为T)、6316(A变为G)。

实施例2：水稻嘉花1号野生型ACCase全长基因的获得

选取嘉花1号野生型植株的叶片，提取基因组DNA，根据NCBI中日本晴ACCase基因(NCBI：XM_015783727)设计引物进行扩增，采用KOD DNA polymerase聚合酶(购自Toyobo公司)扩增ACCase基因，其反应体系如下：

补加无菌水至总体积50μl

PCR扩增反应程序采用两步法，退火和延伸合为一起，采用68度。

程序如下：预变性：94℃3min；35个循环：变性94℃10sec；延伸68℃10min；保温：72℃10min。

取2μl PCR产物经1％琼脂糖凝胶电泳检测，发现有预期大小的片段后(图2)，剩余的PCR产物经PCR清洁试剂盒(购自Axygen公司)清洁回收后，克隆至pMD19-T载体(购自Takara公司)，然后转化大肠杆菌。每个转化随机挑取12个大肠杆菌单克隆进行PCR检测，取PCR结果呈阳性的6个单克隆，送南京一道生物科技有限公司测序，获得突变ACCase基因序列，该基因片段长度为13127bp，通过去除内含子后获得基因全长6984bp，嘉花1号野生型ACCase序列全长基因片段的测序结果如SEQ ID No:21所示。

实施例3：水稻ACCase基因突变位点基因序列的获得

利用点突变试剂盒扩增水稻ACCase基因的突变位点序列。首先，利用引物ACC-CF和ACC-CR，以嘉花1号cDNA为模板，克隆ACCase全长cDNA，连入TK007载体(南京擎科生物科技有限公司)，转化大肠杆菌TOP10菌株感受态细胞，挑选阳性转化子测序验证，获得正确单克隆，质粒命名为TK007-ACCase；第二，利用突变引物ACC-MutF和ACC-MutR，以TK007-ACCase为模版，进行PCR扩增，扩增产物限制性内切酶DpnI消化后，转入大肠杆菌DMT菌株感受态细胞，挑选阳性转化子测序验证，获得ACCase目标位点突变的质粒。

利用突变引物ACC5387-MutF和ACC5387-MutR扩增ACCase-5387突变位点，其核苷酸序列的第5387位由C突变为G，导致其编码的氨基酸序列的第1796位由丙氨酸变为甘氨酸，其核苷酸序列如SEQ ID No:1所示，其编码的氨基酸序列如SEQ ID No:2所示；

利用突变引物ACC5389-MutF和ACC5389-MutR扩增ACCase-5389突变位点，其核苷酸序列的第5389位由G突变为C，导致其编码的氨基酸序列的第1797位由丙氨酸变为脯氨酸，其核苷酸序列如SEQ ID No:3所示，其编码的氨基酸序列如SEQ ID No:4所示；

利用突变引物ACC5504-MutF和ACC5504-MutR扩增ACCase-5504突变位点，其核苷酸序列的第5504位由A突变为C，导致其编码的氨基酸序列的第1835位由谷氨酰胺变为脯氨酸，其核苷酸序列如SEQ ID No:5所示，其编码的氨基酸序列如SEQ ID No:6所示；

利用突变引物ACC5623-MutF和ACC5623-MutR扩增ACCase-5623突变位点，其核苷酸序列的第5623位由G突变为T，导致其编码的氨基酸序列的第1875位由缬氨酸变为苯丙氨酸，其核苷酸序列如SEQ ID No:7所示，其编码的氨基酸序列如SEQ ID No:8所示；

利用突变引物ACC6028-MutF和ACC6028-MutR扩增ACCase-6028突变位点，其核苷酸序列第6028位由T突变为G，导致其编码的氨基酸序列的第2010位由色氨酸变为甘氨酸，其核苷酸序列如SEQ ID No:9所示，其编码的氨基酸序列如SEQ ID No:10所示。

利用突变引物ACC6030-MutF和ACC6030-MutR扩增ACCase-6030突变位点，其核苷酸序列的第6030位由G突变为T，导致其编码的氨基酸序列的第2010位由色氨酸变为半胱氨酸，其核苷酸序列如SEQ ID No:11所示，其编码的氨基酸序列如SEQ ID No:12所示。

利用突变引物ACC6149-MutF和ACC6149-MutR扩增ACCase-6149突变位点，其核苷酸序列的第6149位由A突变为C，导致其编码的氨基酸序列第2050位由谷氨酸变为甘氨酸，其核苷酸序列如SEQ ID No:13所示，其编码的氨基酸序列如SEQ ID No:14所示。

利用突变引物ACC6178-MutF和ACC6178-MutR扩增ACCase-6178突变位点，其核苷酸序列第6178位由G突变为C，导致其编码的氨基酸序列第2060位由缬氨酸变为亮氨酸，其核苷酸序列如SEQ ID No:15所示，其编码的氨基酸序列如SEQ ID No:16所示。

利用突变引物ACC6209-MutF和ACC6209-MutR扩增ACCase-6209突变位点，其核苷酸序列第6209位由C突变为T，导致其编码的氨基酸序列第2070位由丙氨酸变为缬氨酸，其核苷酸序列如SEQ ID No:17所示，其编码的氨基酸序列如SEQ ID No:18所示。

利用突变引物ACC6316-MutF和ACC6316-MutR扩增ACCase-6316突变位点，其核苷酸序列第6316位由A突变为G，导致其编码的氨基酸序列第2106位由赖氨酸变为谷氨酸，其核苷酸序列如SEQ ID No:19所示，其编码的氨基酸序列如SEQ ID No:20所示。

实施例4ACCase转基因水稻的获得及其抗除草剂验证

通过Infuison反应，将突变的ACCase片段连入含有花椰菜花叶病毒35S启动子和35S终止子的植物表达载体中，分别转化野生型水稻。获得纯合并稳定表达含有ACCase突变位点的转基因水稻，并对转基因水稻检测除草剂的耐受性。

将转基因水稻和野生型水稻分别播种，待水稻长至三叶期时，分别喷施乙酰辅酶A羧化酶抑制剂类除草剂喹禾灵(喷施浓度为施用1.5mL喹禾灵/L水(2倍推荐使用浓度))、吡氟氯禾灵(喷施浓度为施用1.5mL吡氟氯禾灵/L水(1倍推荐使用浓度))、烯草酮(喷施浓度为施用1.5mL烯草酮/L水(1倍推荐使用浓度))、吡氟禾草灵(喷施浓度为施用1.5mL吡氟禾草灵/L水(1倍推荐使用浓度))，喷施一个月后调查枯死情况，发现野生型对照在喷施和以上同样浓度的乙酰辅酶A羧化酶抑制剂类除草剂喹禾灵、吡氟氯禾灵、烯草酮、吡氟禾草灵后，几乎停止生长，新长出来的叶片完全枯黄，并逐渐枯死。而含有ACCase突变位点的转基因水稻则全部对乙酰辅酶A羧化酶抑制剂类除草剂表现出抗的表型，其正常的生长发育几乎没有受到影响，最终正常拔节抽穗。表1中显示本发明转基因水稻对ACCase抑制性除草剂的抗性比野生型要高，伤害百分比要低60％以上。这表明我们获得的携带有ACCase突变位点的转基因水稻确实能够稳定遗传的获得抗乙酰辅酶A羧化酶抑制剂类除草剂的抗性。

表1乙酰辅酶A羧化酶类除草剂对野生型和转基因水稻的伤害比率

尽管本发明的具体实施方式已经得到详细描述，本领域技术人员将会理解，根据已经公开的所有教导，可以对那些细节进行各种修改和替换，这些均在本发明的保护范围内。本发明的全部范围由所附专利要求极其任何等同物给出。

序列表

<110> 江苏省农业科学院

<120> 具有除草剂抗性的ACCase突变型基因和蛋白及其应用

<160> 44

<170> SIPOSequenceListing 1.0

<210> 1

<211> 6984

<212> DNA

<213> 水稻(Oryza sativa嘉花1号ACCase-5387突变核苷酸序列)

<400> 1

atgacatcca cacatgtggc gacattggga gttggtgccc aggcacctcc tcgtcaccag 60

aaaaagtcag ctggcactgc atttgtatca tctgggtcat caagaccctc ataccgaaag 120

aatggtcagc gtactcggtc acttagggaa gaaagcaatg gaggagtgtc tgattccaaa 180

aagcttaacc actctattcg ccaaggtctt gctggcatca ttgacctccc aaatgacgca 240

gcttcagaag ttgatatttc acatggttcc gaagatccca gggggcctac ggtcccaggt 300

tcctaccaaa tgaatgggat tatcaatgaa acacataatg ggaggcatgc ttcagtctcc 360

aaggttgttg agttttgtac ggcacttggt ggcaaaacac caattcacag tgtattagtg 420

gccaacaatg gaatggcagc agctaagttc atgcggagtg tccgaacatg ggctaatgat 480

acttttggat cagagaaggc aattcagctg atagctatgg caactccgga ggatctgagg 540

ataaatgcag agcacatcag aattgccgat caatttgtag aggtacctgg tggaacaaac 600

aacaacaact atgcaaatgt ccaactcata gtggagatag cagagagaac aggtgtttct 660

gctgtttggc ctggttgggg tcatgcatct gagaatcctg aacttccaga tgcgctgact 720

gcaaaaggaa ttgtttttct tgggccacca gcatcatcaa tgcatgcatt aggagacaag 780

gttggctcag ctctcattgc tcaagcagct ggagttccaa cacttgcttg gagtggatca 840

catgtggaag ttcctctgga gtgttgcttg gactcaatac ctgatgagat gtatagaaaa 900

gcttgtgtta ctaccacaga ggaagcagtt gcaagttgtc aggtggttgg ttatcctgcc 960

atgattaagg catcttgggg tggtggtggt aaaggaataa ggaaggttca taatgatgat 1020

gaggttagga cattatttaa gcaagttcaa ggcgaagtac ctggttcccc aatatttatc 1080

atgaggctag ctgctcagag tcgacatctt gaagttcagt tgctttgtga tcaatatggc 1140

aacgtagcag cacttcacag tcgagattgc agtgtacaac ggcgacacca aaagataatc 1200

gaggaaggac cagttactgt tgctcctcgt gagactgtga aagagcttga gcaggcagca 1260

cggaggcttg ctaaagctgt gggttatgtt ggtgctgcta ctgttgaata cctttacagc 1320

atggaaactg gtgaatatta ttttctggaa cttaatccac ggctacaggt tgagcatcct 1380

gtcactgagt ggatagctga agtaaatttg cctgcggctc aagttgctgt tggaatgggt 1440

ataccccttt ggcagattcc agagatcagg cgcttctacg gaatgaacca tggaggaggc 1500

tatgaccttt ggaggaaaac agcagctcta gcgactccat ttaactttga tgaagtagat 1560

tctaaatggc caaaaggcca ctgcgtagct gttagaataa ctagcgagga tccagatgat 1620

gggtttaagc ctactggtgg aaaagtaaag gagataagtt tcaagagtaa accaaatgtt 1680

tgggcctatt tctcagtaaa gtctggtgga ggcatccatg aattcgctga ttctcagttc 1740

ggacatgttt ttgcgtatgg aactactaga tcggcagcaa taactaccat ggctcttgca 1800

ctaaaagagg ttcaaattcg tggagaaatt cattcaaacg tagactacac agttgaccta 1860

ttaaatgcct cagattttag agaaaataag attcatactg gttggctgga taccaggata 1920

gccatgcgtg ttcaagctga gaggcctcca tggtatattt cagtcgttgg aggggcttta 1980

tataaaacag taactgccaa cacggccact gtttctgatt atgttggtta tcttaccaag 2040

ggccagattc caccaaagca tatatccctt gtctatacga ctgttgcttt gaatatagat 2100

gggaaaaaat atacaatcga tactgtgagg agtggacatg gtagctacag attgcgaatg 2160

aatggatcaa cggttgacgc aaatgtacaa atattatgtg atggtgggct tttaatgcag 2220

ctggatggaa acagccatgt aatttatgct gaagaagagg ccagtggtac acgacttctt 2280

attgatggaa agacatgcat gttacagaat gaccatgacc catcaaagtt attagctgag 2340

acaccatgca aacttcttcg tttcttggtt gctgatggtg ctcatgttga tgctgatgta 2400

ccatatgcgg aagttgaggt tatgaagatg tgcatgcccc tcttatcacc cgcttctggt 2460

gtcatacatg ttgtaatgtc tgagggccaa gcaatgcagg ctggtgatct tatagctagg 2520

ctggatcttg atgacccttc tgctgttaag agagctgagc cgttcgaaga tacttttcca 2580

caaatgggtc tccctattgc tgcttctggc caagttcaca aattatgtgc tgcaagtctg 2640

aatgcttgtc gaatgatcct tgcggggtat gagcatgata ttgacaaggt tgtgccagag 2700

ttggtatact gcctagacac tccggagctt cctttcctgc agtgggagga gcttatgtct 2760

gttttagcaa ctagacttcc aagaaatctt aaaagtgagt tggagggcaa atatgaggaa 2820

tacaaagtaa aatttgactc tgggataatc aatgatttcc ctgccaatat gctacgagtg 2880

ataattgagg aaaatcttgc atgtggttct gagaaggaga aggctacaaa tgagaggctt 2940

gttgagcctc ttatgagcct actgaagtca tatgagggtg ggagagaaag tcatgctcac 3000

tttgttgtca agtccctttt tgaggagtat ctctatgttg aagaattgtt cagtgatgga 3060

attcagtctg atgtgattga gcgtctgcgc cttcaacata gtaaagacct acagaaggtc 3120

gtagacattg tgttgtccca ccagagtgtt agaaataaaa ctaagctgat actaaaactc 3180

atggagagtc tggtctatcc aaatcctgct gcctacaggg atcaattgat tcgcttttct 3240

tcccttaatc acaaagcgta ttacaagttg gcacttaaag ctagtgaact tcttgaacaa 3300

acaaaactta gtgagctccg tgcaagaata gcaaggagcc tttcagagct ggagatgttt 3360

actgaggaaa gcaagggtct ctccatgcat aagcgagaaa ttgccattaa ggagagcatg 3420

gaagatttag tcactgctcc actgccagtt gaagatgcgc tcatttcttt atttgattgt 3480

agtgatacaa ctgttcaaca gagagtgatt gagacttata tagctcgatt ataccagcct 3540

catcttgtaa aggacagtat caaaatgaaa tggatagaat cgggtgttat tgctttatgg 3600

gaatttcctg aagggcattt tgatgcaaga aatggaggag cggttcttgg tgacaaaaga 3660

tggggtgcca tggtcattgt caagtctctt gaatcacttt caatggccat tagatttgca 3720

ctaaaggaga catcacacta cactagctct gagggcaata tgatgcatat tgctttgttg 3780

ggtgctgata ataagatgca tataattcaa gaaagtggtg atgatgctga cagaatagcc 3840

aaacttccct tgatactaaa ggataatgta accgatctgc atgcctctgg tgtgaaaaca 3900

ataagtttca ttgttcaaag agatgaagca cggatgacaa tgcgtcgtac cttcctttgg 3960

tctgatgaaa agctttctta tgaggaagag ccaattctcc ggcatgtgga acctcctctt 4020

tctgcacttc ttgagttgga caagttgaaa gtgaaaggat acaatgaaat gaagtatacc 4080

ccatcacggg atcgtcaatg gcatatctac acacttagaa atactgaaaa ccccaaaatg 4140

ttgcaccggg tatttttccg aacccttgtc aggcaaccca gtgtatccaa caagttttct 4200

tcgggccaga ttggtgacat ggaagttggg agtgctgaag aacctctgtc atttacatca 4260

accagcatat taagatcttt gatgactgct atagaggaat tggagcttca cgcaattaga 4320

actggccatt cacacatgta tttgcatgta ttgaaagaac aaaagcttct tgatcttgtt 4380

ccagtttcag ggaatacagt tttggatgtt ggtcaagatg aagctactgc atattcactt 4440

ttaaaagaaa tggctatgaa gatacatgaa cttgttggtg caagaatgca ccatctttct 4500

gtatgccaat gggaagtgaa acttaagttg gactgcgatg gtcctgccag tggtacctgg 4560

aggattgtaa caaccaatgt tactagtcac acttgcactg tggatatcta ccgtgagatg 4620

gaagataaag aatcacggaa gttagtatac catcccgcca ctccggcggc tggtcctctg 4680

catggtgtgg cactgaataa tccatatcag cctttgagtg tcattgatct caaacgctgt 4740

tctgctagga ataatagaac tacatactgc tatgattttc cactggcatt tgaaactgca 4800

gtgaggaagt catggtcctc tagtacctct ggtgcttcta aaggtgttga aaatgcccaa 4860

tgttatgtta aagctacaga gttggtattt gcggacaaac atgggtcatg gggcactcct 4920

ttagttcaaa tggaccggcc tgctgggctc aatgacattg gtatggtagc ttggaccttg 4980

aagatgtcca ctcctgaatt tcctagtggt agggagatta ttgttgttgc aaatgatatt 5040

acgttcagag ctggatcatt tggcccaagg gaagatgcat tttttgaagc tgttaccaac 5100

ctagcctgtg agaagaaact tcctcttatt tatttggcag caaattctgg tgctcgaatt 5160

ggcatagcag atgaagtgaa atcttgcttc cgtgttgggt ggtctgatga tggcagccct 5220

gaacgtgggt ttcagtacat ttatctaagc gaagaagact atgctcgtat tggcacttct 5280

gtcatagcac ataagatgca gctagacagt ggtgaaatta ggtgggttat tgattctgtt 5340

gtgggcaagg aagatggact tggtgtggag aatatacatg gaagtggtgc tattgccagt 5400

gcttattcta gggcatataa ggagacattt acacttacat ttgtgactgg aagaactgtt 5460

ggaataggag cttatcttgc tcgacttggc atccggtgca tacagcgtct tgaccagcct 5520

attattctta caggctattc tgcactgaac aagcttcttg ggcgggaagt gtacagctcc 5580

cacatgcagt tgggtggtcc caaaatcatg gcaactaatg gtgttgtcca tcttactgtt 5640

tcagatgacc ttgaaggcgt ttctaatata ttgaggtggc tcagttatgt tcctgcctac 5700

attggtggac cacttccagt aacaacaccg ttggacccac cggacagacc tgttgcatac 5760

attcctgaga actcgtgtga tcctcgagcg gctatccgtg gtgttgatga cagccaaggg 5820

aaatggttag gtggtatgtt tgataaagac agctttgtgg aaacatttga aggttgggct 5880

aagacagtgg ttactggcag agcaaagctt ggtggaattc cagtgggtgt gatagctgtg 5940

gagactcaga ccatgatgca aactatccct gctgaccctg gtcagcttga ttcccgtgag 6000

caatctgttc ctcgtgctgg acaagtgtgg tttccagatt ctgcaaccaa gactgcgcag 6060

gcattgctgg acttcaaccg tgaaggatta cctctgttca tcctcgctaa ctggagaggc 6120

ttctctggtg gacaaagaga tctttttgaa ggaattcttc aggctggctc gactattgtt 6180

gagaacctta ggacatacaa tcagcctgcc tttgtctaca ttcccatggc tgcagagcta 6240

cgaggagggg cttgggttgt ggttgatagc aagataaacc cagaccgcat tgagtgctat 6300

gctgagagga ctgcaaaagg caatgttctg gaaccgcaag ggttaattga gatcaagttc 6360

aggtcagagg aactccagga ttgcatgagt cggcttgacc caacattaat tgatctgaaa 6420

gcaaaactcg aagtagcaaa taaaaatgga agtgctgaca caaaatcgct tcaagaaaat 6480

atagaagctc gaacaaaaca gttgatgcct ctatatactc agattgcgat acggtttgct 6540

gaattgcatg atacatccct cagaatggct gcgaaaggtg tgattaagaa agttgtggac 6600

tgggaagaat cacgatcttt cttctataag agattacgga ggaggatctc tgaggatgtt 6660

cttgcaaaag aaattagagc tgtagcaggt gagcagtttt cccaccaacc agcaatcgag 6720

ctgatcaaga aatggtattc agcttcacat gcagctgaat gggatgatga cgatgctttt 6780

gttgcttgga tggataaccc tgaaaactac aaggattata ttcaatatct taaggctcaa 6840

agagtatccc aatccctctc aagtctttca gattccagct cagatttgca agccctgcca 6900

cagggtcttt ccatgttact agataagatg gatccctcta gaagagctca acttgttgaa 6960

gaaatcagga aggtccttgg ttga 6984

<210> 2

<211> 2327

<212> PRT

<213> 水稻(Oryza sativa嘉花1号ACCase-5387突变氨基酸序列)

<400> 2

Met Thr Ser Thr His Val Ala Thr Leu Gly Val Gly Ala Gln Ala Pro

1 5 10 15

Pro Arg His Gln Lys Lys Ser Ala Gly Thr Ala Phe Val Ser Ser Gly

20 25 30

Ser Ser Arg Pro Ser Tyr Arg Lys Asn Gly Gln Arg Thr Arg Ser Leu

35 40 45

Arg Glu Glu Ser Asn Gly Gly Val Ser Asp Ser Lys Lys Leu Asn His

50 55 60

Ser Ile Arg Gln Gly Leu Ala Gly Ile Ile Asp Leu Pro Asn Asp Ala

65 70 75 80

Ala Ser Glu Val Asp Ile Ser His Gly Ser Glu Asp Pro Arg Gly Pro

85 90 95

Thr Val Pro Gly Ser Tyr Gln Met Asn Gly Ile Ile Asn Glu Thr His

100 105 110

Asn Gly Arg His Ala Ser Val Ser Lys Val Val Glu Phe Cys Thr Ala

115 120 125

Leu Gly Gly Lys Thr Pro Ile His Ser Val Leu Val Ala Asn Asn Gly

130 135 140

Met Ala Ala Ala Lys Phe Met Arg Ser Val Arg Thr Trp Ala Asn Asp

145 150 155 160

Thr Phe Gly Ser Glu Lys Ala Ile Gln Leu Ile Ala Met Ala Thr Pro

165 170 175

Glu Asp Leu Arg Ile Asn Ala Glu His Ile Arg Ile Ala Asp Gln Phe

180 185 190

Val Glu Val Pro Gly Gly Thr Asn Asn Asn Asn Tyr Ala Asn Val Gln

195 200 205

Leu Ile Val Glu Ile Ala Glu Arg Thr Gly Val Ser Ala Val Trp Pro

210 215 220

Gly Trp Gly His Ala Ser Glu Asn Pro Glu Leu Pro Asp Ala Leu Thr

225 230 235 240

Ala Lys Gly Ile Val Phe Leu Gly Pro Pro Ala Ser Ser Met His Ala

245 250 255

Leu Gly Asp Lys Val Gly Ser Ala Leu Ile Ala Gln Ala Ala Gly Val

260 265 270

Pro Thr Leu Ala Trp Ser Gly Ser His Val Glu Val Pro Leu Glu Cys

275 280 285

Cys Leu Asp Ser Ile Pro Asp Glu Met Tyr Arg Lys Ala Cys Val Thr

290 295 300

Thr Thr Glu Glu Ala Val Ala Ser Cys Gln Val Val Gly Tyr Pro Ala

305 310 315 320

Met Ile Lys Ala Ser Trp Gly Gly Gly Gly Lys Gly Ile Arg Lys Val

325 330 335

His Asn Asp Asp Glu Val Arg Thr Leu Phe Lys Gln Val Gln Gly Glu

340 345 350

Val Pro Gly Ser Pro Ile Phe Ile Met Arg Leu Ala Ala Gln Ser Arg

355 360 365

His Leu Glu Val Gln Leu Leu Cys Asp Gln Tyr Gly Asn Val Ala Ala

370 375 380

Leu His Ser Arg Asp Cys Ser Val Gln Arg Arg His Gln Lys Ile Ile

385 390 395 400

Glu Glu Gly Pro Val Thr Val Ala Pro Arg Glu Thr Val Lys Glu Leu

405 410 415

Glu Gln Ala Ala Arg Arg Leu Ala Lys Ala Val Gly Tyr Val Gly Ala

420 425 430

Ala Thr Val Glu Tyr Leu Tyr Ser Met Glu Thr Gly Glu Tyr Tyr Phe

435 440 445

Leu Glu Leu Asn Pro Arg Leu Gln Val Glu His Pro Val Thr Glu Trp

450 455 460

Ile Ala Glu Val Asn Leu Pro Ala Ala Gln Val Ala Val Gly Met Gly

465 470 475 480

Ile Pro Leu Trp Gln Ile Pro Glu Ile Arg Arg Phe Tyr Gly Met Asn

485 490 495

His Gly Gly Gly Tyr Asp Leu Trp Arg Lys Thr Ala Ala Leu Ala Thr

500 505 510

Pro Phe Asn Phe Asp Glu Val Asp Ser Lys Trp Pro Lys Gly His Cys

515 520 525

Val Ala Val Arg Ile Thr Ser Glu Asp Pro Asp Asp Gly Phe Lys Pro

530 535 540

Thr Gly Gly Lys Val Lys Glu Ile Ser Phe Lys Ser Lys Pro Asn Val

545 550 555 560

Trp Ala Tyr Phe Ser Val Lys Ser Gly Gly Gly Ile His Glu Phe Ala

565 570 575

Asp Ser Gln Phe Gly His Val Phe Ala Tyr Gly Thr Thr Arg Ser Ala

580 585 590

Ala Ile Thr Thr Met Ala Leu Ala Leu Lys Glu Val Gln Ile Arg Gly

595 600 605

Glu Ile His Ser Asn Val Asp Tyr Thr Val Asp Leu Leu Asn Ala Ser

610 615 620

Asp Phe Arg Glu Asn Lys Ile His Thr Gly Trp Leu Asp Thr Arg Ile

625 630 635 640

Ala Met Arg Val Gln Ala Glu Arg Pro Pro Trp Tyr Ile Ser Val Val

645 650 655

Gly Gly Ala Leu Tyr Lys Thr Val Thr Ala Asn Thr Ala Thr Val Ser

660 665 670

Asp Tyr Val Gly Tyr Leu Thr Lys Gly Gln Ile Pro Pro Lys His Ile

675 680 685

Ser Leu Val Tyr Thr Thr Val Ala Leu Asn Ile Asp Gly Lys Lys Tyr

690 695 700

Thr Ile Asp Thr Val Arg Ser Gly His Gly Ser Tyr Arg Leu Arg Met

705 710 715 720

Asn Gly Ser Thr Val Asp Ala Asn Val Gln Ile Leu Cys Asp Gly Gly

725 730 735

Leu Leu Met Gln Leu Asp Gly Asn Ser His Val Ile Tyr Ala Glu Glu

740 745 750

Glu Ala Ser Gly Thr Arg Leu Leu Ile Asp Gly Lys Thr Cys Met Leu

755 760 765

Gln Asn Asp His Asp Pro Ser Lys Leu Leu Ala Glu Thr Pro Cys Lys

770 775 780

Leu Leu Arg Phe Leu Val Ala Asp Gly Ala His Val Asp Ala Asp Val

785 790 795 800

Pro Tyr Ala Glu Val Glu Val Met Lys Met Cys Met Pro Leu Leu Ser

805 810 815

Pro Ala Ser Gly Val Ile His Val Val Met Ser Glu Gly Gln Ala Met

820 825 830

Gln Ala Gly Asp Leu Ile Ala Arg Leu Asp Leu Asp Asp Pro Ser Ala

835 840 845

Val Lys Arg Ala Glu Pro Phe Glu Asp Thr Phe Pro Gln Met Gly Leu

850 855 860

Pro Ile Ala Ala Ser Gly Gln Val His Lys Leu Cys Ala Ala Ser Leu

865 870 875 880

Asn Ala Cys Arg Met Ile Leu Ala Gly Tyr Glu His Asp Ile Asp Lys

885 890 895

Val Val Pro Glu Leu Val Tyr Cys Leu Asp Thr Pro Glu Leu Pro Phe

900 905 910

Leu Gln Trp Glu Glu Leu Met Ser Val Leu Ala Thr Arg Leu Pro Arg

915 920 925

Asn Leu Lys Ser Glu Leu Glu Gly Lys Tyr Glu Glu Tyr Lys Val Lys

930 935 940

Phe Asp Ser Gly Ile Ile Asn Asp Phe Pro Ala Asn Met Leu Arg Val

945 950 955 960

Ile Ile Glu Glu Asn Leu Ala Cys Gly Ser Glu Lys Glu Lys Ala Thr

965 970 975

Asn Glu Arg Leu Val Glu Pro Leu Met Ser Leu Leu Lys Ser Tyr Glu

980 985 990

Gly Gly Arg Glu Ser His Ala His Phe Val Val Lys Ser Leu Phe Glu

995 1000 1005

Glu Tyr Leu Tyr Val Glu Glu Leu Phe Ser Asp Gly Ile Gln Ser Asp

1010 1015 1020

Val Ile Glu Arg Leu Arg Leu Gln His Ser Lys Asp Leu Gln Lys Val

1025 1030 1035 1040

Val Asp Ile Val Leu Ser His Gln Ser Val Arg Asn Lys Thr Lys Leu

1045 1050 1055

Ile Leu Lys Leu Met Glu Ser Leu Val Tyr Pro Asn Pro Ala Ala Tyr

1060 1065 1070

Arg Asp Gln Leu Ile Arg Phe Ser Ser Leu Asn His Lys Ala Tyr Tyr

1075 1080 1085

Lys Leu Ala Leu Lys Ala Ser Glu Leu Leu Glu Gln Thr Lys Leu Ser

1090 1095 1100

Glu Leu Arg Ala Arg Ile Ala Arg Ser Leu Ser Glu Leu Glu Met Phe

1105 1110 1115 1120

Thr Glu Glu Ser Lys Gly Leu Ser Met His Lys Arg Glu Ile Ala Ile

1125 1130 1135

Lys Glu Ser Met Glu Asp Leu Val Thr Ala Pro Leu Pro Val Glu Asp

1140 1145 1150

Ala Leu Ile Ser Leu Phe Asp Cys Ser Asp Thr Thr Val Gln Gln Arg

1155 1160 1165

Val Ile Glu Thr Tyr Ile Ala Arg Leu Tyr Gln Pro His Leu Val Lys

1170 1175 1180

Asp Ser Ile Lys Met Lys Trp Ile Glu Ser Gly Val Ile Ala Leu Trp

1185 1190 1195 1200

Glu Phe Pro Glu Gly His Phe Asp Ala Arg Asn Gly Gly Ala Val Leu

1205 1210 1215

Gly Asp Lys Arg Trp Gly Ala Met Val Ile Val Lys Ser Leu Glu Ser

1220 1225 1230

Leu Ser Met Ala Ile Arg Phe Ala Leu Lys Glu Thr Ser His Tyr Thr

1235 1240 1245

Ser Ser Glu Gly Asn Met Met His Ile Ala Leu Leu Gly Ala Asp Asn

1250 1255 1260

Lys Met His Ile Ile Gln Glu Ser Gly Asp Asp Ala Asp Arg Ile Ala

1265 1270 1275 1280

Lys Leu Pro Leu Ile Leu Lys Asp Asn Val Thr Asp Leu His Ala Ser

1285 1290 1295

Gly Val Lys Thr Ile Ser Phe Ile Val Gln Arg Asp Glu Ala Arg Met

1300 1305 1310

Thr Met Arg Arg Thr Phe Leu Trp Ser Asp Glu Lys Leu Ser Tyr Glu

1315 1320 1325

Glu Glu Pro Ile Leu Arg His Val Glu Pro Pro Leu Ser Ala Leu Leu

1330 1335 1340

Glu Leu Asp Lys Leu Lys Val Lys Gly Tyr Asn Glu Met Lys Tyr Thr

1345 1350 1355 1360

Pro Ser Arg Asp Arg Gln Trp His Ile Tyr Thr Leu Arg Asn Thr Glu

1365 1370 1375

Asn Pro Lys Met Leu His Arg Val Phe Phe Arg Thr Leu Val Arg Gln

1380 1385 1390

Pro Ser Val Ser Asn Lys Phe Ser Ser Gly Gln Ile Gly Asp Met Glu

1395 1400 1405

Val Gly Ser Ala Glu Glu Pro Leu Ser Phe Thr Ser Thr Ser Ile Leu

1410 1415 1420

Arg Ser Leu Met Thr Ala Ile Glu Glu Leu Glu Leu His Ala Ile Arg

1425 1430 1435 1440

Thr Gly His Ser His Met Tyr Leu His Val Leu Lys Glu Gln Lys Leu

1445 1450 1455

Leu Asp Leu Val Pro Val Ser Gly Asn Thr Val Leu Asp Val Gly Gln

1460 1465 1470

Asp Glu Ala Thr Ala Tyr Ser Leu Leu Lys Glu Met Ala Met Lys Ile

1475 1480 1485

His Glu Leu Val Gly Ala Arg Met His His Leu Ser Val Cys Gln Trp

1490 1495 1500

Glu Val Lys Leu Lys Leu Asp Cys Asp Gly Pro Ala Ser Gly Thr Trp

1505 1510 1515 1520

Arg Ile Val Thr Thr Asn Val Thr Ser His Thr Cys Thr Val Asp Ile

1525 1530 1535

Tyr Arg Glu Met Glu Asp Lys Glu Ser Arg Lys Leu Val Tyr His Pro

1540 1545 1550

Ala Thr Pro Ala Ala Gly Pro Leu His Gly Val Ala Leu Asn Asn Pro

1555 1560 1565

Tyr Gln Pro Leu Ser Val Ile Asp Leu Lys Arg Cys Ser Ala Arg Asn

1570 1575 1580

Asn Arg Thr Thr Tyr Cys Tyr Asp Phe Pro Leu Ala Phe Glu Thr Ala

1585 1590 1595 1600

Val Arg Lys Ser Trp Ser Ser Ser Thr Ser Gly Ala Ser Lys Gly Val

1605 1610 1615

Glu Asn Ala Gln Cys Tyr Val Lys Ala Thr Glu Leu Val Phe Ala Asp

1620 1625 1630

Lys His Gly Ser Trp Gly Thr Pro Leu Val Gln Met Asp Arg Pro Ala

1635 1640 1645

Gly Leu Asn Asp Ile Gly Met Val Ala Trp Thr Leu Lys Met Ser Thr

1650 1655 1660

Pro Glu Phe Pro Ser Gly Arg Glu Ile Ile Val Val Ala Asn Asp Ile

1665 1670 1675 1680

Thr Phe Arg Ala Gly Ser Phe Gly Pro Arg Glu Asp Ala Phe Phe Glu

1685 1690 1695

Ala Val Thr Asn Leu Ala Cys Glu Lys Lys Leu Pro Leu Ile Tyr Leu

1700 1705 1710

Ala Ala Asn Ser Gly Ala Arg Ile Gly Ile Ala Asp Glu Val Lys Ser

1715 1720 1725

Cys Phe Arg Val Gly Trp Ser Asp Asp Gly Ser Pro Glu Arg Gly Phe

1730 1735 1740

Gln Tyr Ile Tyr Leu Ser Glu Glu Asp Tyr Ala Arg Ile Gly Thr Ser

1745 1750 1755 1760

Val Ile Ala His Lys Met Gln Leu Asp Ser Gly Glu Ile Arg Trp Val

1765 1770 1775

Ile Asp Ser Val Val Gly Lys Glu Asp Gly Leu Gly Val Glu Asn Ile

1780 1785 1790

His Gly Ser Gly Ala Ile Ala Ser Ala Tyr Ser Arg Ala Tyr Lys Glu

1795 1800 1805

Thr Phe Thr Leu Thr Phe Val Thr Gly Arg Thr Val Gly Ile Gly Ala

1810 1815 1820

Tyr Leu Ala Arg Leu Gly Ile Arg Cys Ile Gln Arg Leu Asp Gln Pro

1825 1830 1835 1840

Ile Ile Leu Thr Gly Tyr Ser Ala Leu Asn Lys Leu Leu Gly Arg Glu

1845 1850 1855

Val Tyr Ser Ser His Met Gln Leu Gly Gly Pro Lys Ile Met Ala Thr

1860 1865 1870

Asn Gly Val Val His Leu Thr Val Ser Asp Asp Leu Glu Gly Val Ser

1875 1880 1885

Asn Ile Leu Arg Trp Leu Ser Tyr Val Pro Ala Tyr Ile Gly Gly Pro

1890 1895 1900

Leu Pro Val Thr Thr Pro Leu Asp Pro Pro Asp Arg Pro Val Ala Tyr

1905 1910 1915 1920

Ile Pro Glu Asn Ser Cys Asp Pro Arg Ala Ala Ile Arg Gly Val Asp

1925 1930 1935

Asp Ser Gln Gly Lys Trp Leu Gly Gly Met Phe Asp Lys Asp Ser Phe

1940 1945 1950

Val Glu Thr Phe Glu Gly Trp Ala Lys Thr Val Val Thr Gly Arg Ala

1955 1960 1965

Lys Leu Gly Gly Ile Pro Val Gly Val Ile Ala Val Glu Thr Gln Thr

1970 1975 1980

Met Met Gln Thr Ile Pro Ala Asp Pro Gly Gln Leu Asp Ser Arg Glu

1985 1990 1995 2000

Gln Ser Val Pro Arg Ala Gly Gln Val Trp Phe Pro Asp Ser Ala Thr

2005 2010 2015

Lys Thr Ala Gln Ala Leu Leu Asp Phe Asn Arg Glu Gly Leu Pro Leu

2020 2025 2030

Phe Ile Leu Ala Asn Trp Arg Gly Phe Ser Gly Gly Gln Arg Asp Leu

2035 2040 2045

Phe Glu Gly Ile Leu Gln Ala Gly Ser Thr Ile Val Glu Asn Leu Arg

2050 2055 2060

Thr Tyr Asn Gln Pro Ala Phe Val Tyr Ile Pro Met Ala Ala Glu Leu

2065 2070 2075 2080

Arg Gly Gly Ala Trp Val Val Val Asp Ser Lys Ile Asn Pro Asp Arg

2085 2090 2095

Ile Glu Cys Tyr Ala Glu Arg Thr Ala Lys Gly Asn Val Leu Glu Pro

2100 2105 2110

Gln Gly Leu Ile Glu Ile Lys Phe Arg Ser Glu Glu Leu Gln Asp Cys

2115 2120 2125

Met Ser Arg Leu Asp Pro Thr Leu Ile Asp Leu Lys Ala Lys Leu Glu

2130 2135 2140

Val Ala Asn Lys Asn Gly Ser Ala Asp Thr Lys Ser Leu Gln Glu Asn

2145 2150 2155 2160

Ile Glu Ala Arg Thr Lys Gln Leu Met Pro Leu Tyr Thr Gln Ile Ala

2165 2170 2175

Ile Arg Phe Ala Glu Leu His Asp Thr Ser Leu Arg Met Ala Ala Lys

2180 2185 2190

Gly Val Ile Lys Lys Val Val Asp Trp Glu Glu Ser Arg Ser Phe Phe

2195 2200 2205

Tyr Lys Arg Leu Arg Arg Arg Ile Ser Glu Asp Val Leu Ala Lys Glu

2210 2215 2220

Ile Arg Ala Val Ala Gly Glu Gln Phe Ser His Gln Pro Ala Ile Glu

2225 2230 2235 2240

Leu Ile Lys Lys Trp Tyr Ser Ala Ser His Ala Ala Glu Trp Asp Asp

2245 2250 2255

Asp Asp Ala Phe Val Ala Trp Met Asp Asn Pro Glu Asn Tyr Lys Asp

2260 2265 2270

Tyr Ile Gln Tyr Leu Lys Ala Gln Arg Val Ser Gln Ser Leu Ser Ser

2275 2280 2285

Leu Ser Asp Ser Ser Ser Asp Leu Gln Ala Leu Pro Gln Gly Leu Ser

2290 2295 2300

Met Leu Leu Asp Lys Met Asp Pro Ser Arg Arg Ala Gln Leu Val Glu

2305 2310 2315 2320

Glu Ile Arg Lys Val Leu Gly

2325

<210> 3

<211> 6984

<212> DNA

<213> 水稻(Oryza sativa嘉花1号ACCase-5389突变核苷酸序列)

<400> 3

atgacatcca cacatgtggc gacattggga gttggtgccc aggcacctcc tcgtcaccag 60

aaaaagtcag ctggcactgc atttgtatca tctgggtcat caagaccctc ataccgaaag 120

aatggtcagc gtactcggtc acttagggaa gaaagcaatg gaggagtgtc tgattccaaa 180

aagcttaacc actctattcg ccaaggtctt gctggcatca ttgacctccc aaatgacgca 240

gcttcagaag ttgatatttc acatggttcc gaagatccca gggggcctac ggtcccaggt 300

tcctaccaaa tgaatgggat tatcaatgaa acacataatg ggaggcatgc ttcagtctcc 360

aaggttgttg agttttgtac ggcacttggt ggcaaaacac caattcacag tgtattagtg 420

gccaacaatg gaatggcagc agctaagttc atgcggagtg tccgaacatg ggctaatgat 480

acttttggat cagagaaggc aattcagctg atagctatgg caactccgga ggatctgagg 540

ataaatgcag agcacatcag aattgccgat caatttgtag aggtacctgg tggaacaaac 600

aacaacaact atgcaaatgt ccaactcata gtggagatag cagagagaac aggtgtttct 660

gctgtttggc ctggttgggg tcatgcatct gagaatcctg aacttccaga tgcgctgact 720

gcaaaaggaa ttgtttttct tgggccacca gcatcatcaa tgcatgcatt aggagacaag 780

gttggctcag ctctcattgc tcaagcagct ggagttccaa cacttgcttg gagtggatca 840

catgtggaag ttcctctgga gtgttgcttg gactcaatac ctgatgagat gtatagaaaa 900

gcttgtgtta ctaccacaga ggaagcagtt gcaagttgtc aggtggttgg ttatcctgcc 960

atgattaagg catcttgggg tggtggtggt aaaggaataa ggaaggttca taatgatgat 1020

gaggttagga cattatttaa gcaagttcaa ggcgaagtac ctggttcccc aatatttatc 1080

atgaggctag ctgctcagag tcgacatctt gaagttcagt tgctttgtga tcaatatggc 1140

aacgtagcag cacttcacag tcgagattgc agtgtacaac ggcgacacca aaagataatc 1200

gaggaaggac cagttactgt tgctcctcgt gagactgtga aagagcttga gcaggcagca 1260

cggaggcttg ctaaagctgt gggttatgtt ggtgctgcta ctgttgaata cctttacagc 1320

atggaaactg gtgaatatta ttttctggaa cttaatccac ggctacaggt tgagcatcct 1380

gtcactgagt ggatagctga agtaaatttg cctgcggctc aagttgctgt tggaatgggt 1440

ataccccttt ggcagattcc agagatcagg cgcttctacg gaatgaacca tggaggaggc 1500

tatgaccttt ggaggaaaac agcagctcta gcgactccat ttaactttga tgaagtagat 1560

tctaaatggc caaaaggcca ctgcgtagct gttagaataa ctagcgagga tccagatgat 1620

gggtttaagc ctactggtgg aaaagtaaag gagataagtt tcaagagtaa accaaatgtt 1680

tgggcctatt tctcagtaaa gtctggtgga ggcatccatg aattcgctga ttctcagttc 1740

ggacatgttt ttgcgtatgg aactactaga tcggcagcaa taactaccat ggctcttgca 1800

ctaaaagagg ttcaaattcg tggagaaatt cattcaaacg tagactacac agttgaccta 1860

ttaaatgcct cagattttag agaaaataag attcatactg gttggctgga taccaggata 1920

gccatgcgtg ttcaagctga gaggcctcca tggtatattt cagtcgttgg aggggcttta 1980

tataaaacag taactgccaa cacggccact gtttctgatt atgttggtta tcttaccaag 2040

ggccagattc caccaaagca tatatccctt gtctatacga ctgttgcttt gaatatagat 2100

gggaaaaaat atacaatcga tactgtgagg agtggacatg gtagctacag attgcgaatg 2160

aatggatcaa cggttgacgc aaatgtacaa atattatgtg atggtgggct tttaatgcag 2220

ctggatggaa acagccatgt aatttatgct gaagaagagg ccagtggtac acgacttctt 2280

attgatggaa agacatgcat gttacagaat gaccatgacc catcaaagtt attagctgag 2340

acaccatgca aacttcttcg tttcttggtt gctgatggtg ctcatgttga tgctgatgta 2400

ccatatgcgg aagttgaggt tatgaagatg tgcatgcccc tcttatcacc cgcttctggt 2460

gtcatacatg ttgtaatgtc tgagggccaa gcaatgcagg ctggtgatct tatagctagg 2520

ctggatcttg atgacccttc tgctgttaag agagctgagc cgttcgaaga tacttttcca 2580

caaatgggtc tccctattgc tgcttctggc caagttcaca aattatgtgc tgcaagtctg 2640

aatgcttgtc gaatgatcct tgcggggtat gagcatgata ttgacaaggt tgtgccagag 2700

ttggtatact gcctagacac tccggagctt cctttcctgc agtgggagga gcttatgtct 2760

gttttagcaa ctagacttcc aagaaatctt aaaagtgagt tggagggcaa atatgaggaa 2820

tacaaagtaa aatttgactc tgggataatc aatgatttcc ctgccaatat gctacgagtg 2880

ataattgagg aaaatcttgc atgtggttct gagaaggaga aggctacaaa tgagaggctt 2940

gttgagcctc ttatgagcct actgaagtca tatgagggtg ggagagaaag tcatgctcac 3000

tttgttgtca agtccctttt tgaggagtat ctctatgttg aagaattgtt cagtgatgga 3060

attcagtctg atgtgattga gcgtctgcgc cttcaacata gtaaagacct acagaaggtc 3120

gtagacattg tgttgtccca ccagagtgtt agaaataaaa ctaagctgat actaaaactc 3180

atggagagtc tggtctatcc aaatcctgct gcctacaggg atcaattgat tcgcttttct 3240

tcccttaatc acaaagcgta ttacaagttg gcacttaaag ctagtgaact tcttgaacaa 3300

acaaaactta gtgagctccg tgcaagaata gcaaggagcc tttcagagct ggagatgttt 3360

actgaggaaa gcaagggtct ctccatgcat aagcgagaaa ttgccattaa ggagagcatg 3420

gaagatttag tcactgctcc actgccagtt gaagatgcgc tcatttcttt atttgattgt 3480

agtgatacaa ctgttcaaca gagagtgatt gagacttata tagctcgatt ataccagcct 3540

catcttgtaa aggacagtat caaaatgaaa tggatagaat cgggtgttat tgctttatgg 3600

gaatttcctg aagggcattt tgatgcaaga aatggaggag cggttcttgg tgacaaaaga 3660

tggggtgcca tggtcattgt caagtctctt gaatcacttt caatggccat tagatttgca 3720

ctaaaggaga catcacacta cactagctct gagggcaata tgatgcatat tgctttgttg 3780

ggtgctgata ataagatgca tataattcaa gaaagtggtg atgatgctga cagaatagcc 3840

aaacttccct tgatactaaa ggataatgta accgatctgc atgcctctgg tgtgaaaaca 3900

ataagtttca ttgttcaaag agatgaagca cggatgacaa tgcgtcgtac cttcctttgg 3960

tctgatgaaa agctttctta tgaggaagag ccaattctcc ggcatgtgga acctcctctt 4020

tctgcacttc ttgagttgga caagttgaaa gtgaaaggat acaatgaaat gaagtatacc 4080

ccatcacggg atcgtcaatg gcatatctac acacttagaa atactgaaaa ccccaaaatg 4140

ttgcaccggg tatttttccg aacccttgtc aggcaaccca gtgtatccaa caagttttct 4200

tcgggccaga ttggtgacat ggaagttggg agtgctgaag aacctctgtc atttacatca 4260

accagcatat taagatcttt gatgactgct atagaggaat tggagcttca cgcaattaga 4320

actggccatt cacacatgta tttgcatgta ttgaaagaac aaaagcttct tgatcttgtt 4380

ccagtttcag ggaatacagt tttggatgtt ggtcaagatg aagctactgc atattcactt 4440

ttaaaagaaa tggctatgaa gatacatgaa cttgttggtg caagaatgca ccatctttct 4500

gtatgccaat gggaagtgaa acttaagttg gactgcgatg gtcctgccag tggtacctgg 4560

aggattgtaa caaccaatgt tactagtcac acttgcactg tggatatcta ccgtgagatg 4620

gaagataaag aatcacggaa gttagtatac catcccgcca ctccggcggc tggtcctctg 4680

catggtgtgg cactgaataa tccatatcag cctttgagtg tcattgatct caaacgctgt 4740

tctgctagga ataatagaac tacatactgc tatgattttc cactggcatt tgaaactgca 4800

gtgaggaagt catggtcctc tagtacctct ggtgcttcta aaggtgttga aaatgcccaa 4860

tgttatgtta aagctacaga gttggtattt gcggacaaac atgggtcatg gggcactcct 4920

ttagttcaaa tggaccggcc tgctgggctc aatgacattg gtatggtagc ttggaccttg 4980

aagatgtcca ctcctgaatt tcctagtggt agggagatta ttgttgttgc aaatgatatt 5040

acgttcagag ctggatcatt tggcccaagg gaagatgcat tttttgaagc tgttaccaac 5100

ctagcctgtg agaagaaact tcctcttatt tatttggcag caaattctgg tgctcgaatt 5160

ggcatagcag atgaagtgaa atcttgcttc cgtgttgggt ggtctgatga tggcagccct 5220

gaacgtgggt ttcagtacat ttatctaagc gaagaagact atgctcgtat tggcacttct 5280

gtcatagcac ataagatgca gctagacagt ggtgaaatta ggtgggttat tgattctgtt 5340

gtgggcaagg aagatggact tggtgtggag aatatacatg gaagtgctcc tattgccagt 5400

gcttattcta gggcatataa ggagacattt acacttacat ttgtgactgg aagaactgtt 5460

ggaataggag cttatcttgc tcgacttggc atccggtgca tacagcgtct tgaccagcct 5520

attattctta caggctattc tgcactgaac aagcttcttg ggcgggaagt gtacagctcc 5580

cacatgcagt tgggtggtcc caaaatcatg gcaactaatg gtgttgtcca tcttactgtt 5640

tcagatgacc ttgaaggcgt ttctaatata ttgaggtggc tcagttatgt tcctgcctac 5700

attggtggac cacttccagt aacaacaccg ttggacccac cggacagacc tgttgcatac 5760

attcctgaga actcgtgtga tcctcgagcg gctatccgtg gtgttgatga cagccaaggg 5820

aaatggttag gtggtatgtt tgataaagac agctttgtgg aaacatttga aggttgggct 5880

aagacagtgg ttactggcag agcaaagctt ggtggaattc cagtgggtgt gatagctgtg 5940

gagactcaga ccatgatgca aactatccct gctgaccctg gtcagcttga ttcccgtgag 6000

caatctgttc ctcgtgctgg acaagtgtgg tttccagatt ctgcaaccaa gactgcgcag 6060

gcattgctgg acttcaaccg tgaaggatta cctctgttca tcctcgctaa ctggagaggc 6120

ttctctggtg gacaaagaga tctttttgaa ggaattcttc aggctggctc gactattgtt 6180

gagaacctta ggacatacaa tcagcctgcc tttgtctaca ttcccatggc tgcagagcta 6240

cgaggagggg cttgggttgt ggttgatagc aagataaacc cagaccgcat tgagtgctat 6300

gctgagagga ctgcaaaagg caatgttctg gaaccgcaag ggttaattga gatcaagttc 6360

aggtcagagg aactccagga ttgcatgagt cggcttgacc caacattaat tgatctgaaa 6420

gcaaaactcg aagtagcaaa taaaaatgga agtgctgaca caaaatcgct tcaagaaaat 6480

atagaagctc gaacaaaaca gttgatgcct ctatatactc agattgcgat acggtttgct 6540

gaattgcatg atacatccct cagaatggct gcgaaaggtg tgattaagaa agttgtggac 6600

tgggaagaat cacgatcttt cttctataag agattacgga ggaggatctc tgaggatgtt 6660

cttgcaaaag aaattagagc tgtagcaggt gagcagtttt cccaccaacc agcaatcgag 6720

ctgatcaaga aatggtattc agcttcacat gcagctgaat gggatgatga cgatgctttt 6780

gttgcttgga tggataaccc tgaaaactac aaggattata ttcaatatct taaggctcaa 6840

agagtatccc aatccctctc aagtctttca gattccagct cagatttgca agccctgcca 6900

cagggtcttt ccatgttact agataagatg gatccctcta gaagagctca acttgttgaa 6960

gaaatcagga aggtccttgg ttga 6984

<210> 4

<211> 2327

<212> PRT

<213> 水稻(Oryza sativa嘉花1号ACCase-5389突变氨基酸序列)

<400> 4

Met Thr Ser Thr His Val Ala Thr Leu Gly Val Gly Ala Gln Ala Pro

1 5 10 15

Pro Arg His Gln Lys Lys Ser Ala Gly Thr Ala Phe Val Ser Ser Gly

20 25 30

Ser Ser Arg Pro Ser Tyr Arg Lys Asn Gly Gln Arg Thr Arg Ser Leu

35 40 45

Arg Glu Glu Ser Asn Gly Gly Val Ser Asp Ser Lys Lys Leu Asn His

50 55 60

Ser Ile Arg Gln Gly Leu Ala Gly Ile Ile Asp Leu Pro Asn Asp Ala

65 70 75 80

Ala Ser Glu Val Asp Ile Ser His Gly Ser Glu Asp Pro Arg Gly Pro

85 90 95

Thr Val Pro Gly Ser Tyr Gln Met Asn Gly Ile Ile Asn Glu Thr His

100 105 110

Asn Gly Arg His Ala Ser Val Ser Lys Val Val Glu Phe Cys Thr Ala

115 120 125

Leu Gly Gly Lys Thr Pro Ile His Ser Val Leu Val Ala Asn Asn Gly

130 135 140

Met Ala Ala Ala Lys Phe Met Arg Ser Val Arg Thr Trp Ala Asn Asp

145 150 155 160

Thr Phe Gly Ser Glu Lys Ala Ile Gln Leu Ile Ala Met Ala Thr Pro

165 170 175

Glu Asp Leu Arg Ile Asn Ala Glu His Ile Arg Ile Ala Asp Gln Phe

180 185 190

Val Glu Val Pro Gly Gly Thr Asn Asn Asn Asn Tyr Ala Asn Val Gln

195 200 205

Leu Ile Val Glu Ile Ala Glu Arg Thr Gly Val Ser Ala Val Trp Pro

210 215 220

Gly Trp Gly His Ala Ser Glu Asn Pro Glu Leu Pro Asp Ala Leu Thr

225 230 235 240

Ala Lys Gly Ile Val Phe Leu Gly Pro Pro Ala Ser Ser Met His Ala

245 250 255

Leu Gly Asp Lys Val Gly Ser Ala Leu Ile Ala Gln Ala Ala Gly Val

260 265 270

Pro Thr Leu Ala Trp Ser Gly Ser His Val Glu Val Pro Leu Glu Cys

275 280 285

Cys Leu Asp Ser Ile Pro Asp Glu Met Tyr Arg Lys Ala Cys Val Thr

290 295 300

Thr Thr Glu Glu Ala Val Ala Ser Cys Gln Val Val Gly Tyr Pro Ala

305 310 315 320

Met Ile Lys Ala Ser Trp Gly Gly Gly Gly Lys Gly Ile Arg Lys Val

325 330 335

His Asn Asp Asp Glu Val Arg Thr Leu Phe Lys Gln Val Gln Gly Glu

340 345 350

Val Pro Gly Ser Pro Ile Phe Ile Met Arg Leu Ala Ala Gln Ser Arg

355 360 365

His Leu Glu Val Gln Leu Leu Cys Asp Gln Tyr Gly Asn Val Ala Ala

370 375 380

Leu His Ser Arg Asp Cys Ser Val Gln Arg Arg His Gln Lys Ile Ile

385 390 395 400

Glu Glu Gly Pro Val Thr Val Ala Pro Arg Glu Thr Val Lys Glu Leu

405 410 415

Glu Gln Ala Ala Arg Arg Leu Ala Lys Ala Val Gly Tyr Val Gly Ala

420 425 430

Ala Thr Val Glu Tyr Leu Tyr Ser Met Glu Thr Gly Glu Tyr Tyr Phe

435 440 445

Leu Glu Leu Asn Pro Arg Leu Gln Val Glu His Pro Val Thr Glu Trp

450 455 460

Ile Ala Glu Val Asn Leu Pro Ala Ala Gln Val Ala Val Gly Met Gly

465 470 475 480

Ile Pro Leu Trp Gln Ile Pro Glu Ile Arg Arg Phe Tyr Gly Met Asn

485 490 495

His Gly Gly Gly Tyr Asp Leu Trp Arg Lys Thr Ala Ala Leu Ala Thr

500 505 510

Pro Phe Asn Phe Asp Glu Val Asp Ser Lys Trp Pro Lys Gly His Cys

515 520 525

Val Ala Val Arg Ile Thr Ser Glu Asp Pro Asp Asp Gly Phe Lys Pro

530 535 540

Thr Gly Gly Lys Val Lys Glu Ile Ser Phe Lys Ser Lys Pro Asn Val

545 550 555 560

Trp Ala Tyr Phe Ser Val Lys Ser Gly Gly Gly Ile His Glu Phe Ala

565 570 575

Asp Ser Gln Phe Gly His Val Phe Ala Tyr Gly Thr Thr Arg Ser Ala

580 585 590

Ala Ile Thr Thr Met Ala Leu Ala Leu Lys Glu Val Gln Ile Arg Gly

595 600 605

Glu Ile His Ser Asn Val Asp Tyr Thr Val Asp Leu Leu Asn Ala Ser

610 615 620

Asp Phe Arg Glu Asn Lys Ile His Thr Gly Trp Leu Asp Thr Arg Ile

625 630 635 640

Ala Met Arg Val Gln Ala Glu Arg Pro Pro Trp Tyr Ile Ser Val Val

645 650 655

Gly Gly Ala Leu Tyr Lys Thr Val Thr Ala Asn Thr Ala Thr Val Ser

660 665 670

Asp Tyr Val Gly Tyr Leu Thr Lys Gly Gln Ile Pro Pro Lys His Ile

675 680 685

Ser Leu Val Tyr Thr Thr Val Ala Leu Asn Ile Asp Gly Lys Lys Tyr

690 695 700

Thr Ile Asp Thr Val Arg Ser Gly His Gly Ser Tyr Arg Leu Arg Met

705 710 715 720

Asn Gly Ser Thr Val Asp Ala Asn Val Gln Ile Leu Cys Asp Gly Gly

725 730 735

Leu Leu Met Gln Leu Asp Gly Asn Ser His Val Ile Tyr Ala Glu Glu

740 745 750

Glu Ala Ser Gly Thr Arg Leu Leu Ile Asp Gly Lys Thr Cys Met Leu

755 760 765

Gln Asn Asp His Asp Pro Ser Lys Leu Leu Ala Glu Thr Pro Cys Lys

770 775 780

Leu Leu Arg Phe Leu Val Ala Asp Gly Ala His Val Asp Ala Asp Val

785 790 795 800

Pro Tyr Ala Glu Val Glu Val Met Lys Met Cys Met Pro Leu Leu Ser

805 810 815

Pro Ala Ser Gly Val Ile His Val Val Met Ser Glu Gly Gln Ala Met

820 825 830

Gln Ala Gly Asp Leu Ile Ala Arg Leu Asp Leu Asp Asp Pro Ser Ala

835 840 845

Val Lys Arg Ala Glu Pro Phe Glu Asp Thr Phe Pro Gln Met Gly Leu

850 855 860

Pro Ile Ala Ala Ser Gly Gln Val His Lys Leu Cys Ala Ala Ser Leu

865 870 875 880

Asn Ala Cys Arg Met Ile Leu Ala Gly Tyr Glu His Asp Ile Asp Lys

885 890 895

Val Val Pro Glu Leu Val Tyr Cys Leu Asp Thr Pro Glu Leu Pro Phe

900 905 910

Leu Gln Trp Glu Glu Leu Met Ser Val Leu Ala Thr Arg Leu Pro Arg

915 920 925

Asn Leu Lys Ser Glu Leu Glu Gly Lys Tyr Glu Glu Tyr Lys Val Lys

930 935 940

Phe Asp Ser Gly Ile Ile Asn Asp Phe Pro Ala Asn Met Leu Arg Val

945 950 955 960

Ile Ile Glu Glu Asn Leu Ala Cys Gly Ser Glu Lys Glu Lys Ala Thr

965 970 975

Asn Glu Arg Leu Val Glu Pro Leu Met Ser Leu Leu Lys Ser Tyr Glu

980 985 990

Gly Gly Arg Glu Ser His Ala His Phe Val Val Lys Ser Leu Phe Glu

995 1000 1005

Glu Tyr Leu Tyr Val Glu Glu Leu Phe Ser Asp Gly Ile Gln Ser Asp

1010 1015 1020

Val Ile Glu Arg Leu Arg Leu Gln His Ser Lys Asp Leu Gln Lys Val

1025 1030 1035 1040

Val Asp Ile Val Leu Ser His Gln Ser Val Arg Asn Lys Thr Lys Leu

1045 1050 1055

Ile Leu Lys Leu Met Glu Ser Leu Val Tyr Pro Asn Pro Ala Ala Tyr

1060 1065 1070

Arg Asp Gln Leu Ile Arg Phe Ser Ser Leu Asn His Lys Ala Tyr Tyr

1075 1080 1085

Lys Leu Ala Leu Lys Ala Ser Glu Leu Leu Glu Gln Thr Lys Leu Ser

1090 1095 1100

Glu Leu Arg Ala Arg Ile Ala Arg Ser Leu Ser Glu Leu Glu Met Phe

1105 1110 1115 1120

Thr Glu Glu Ser Lys Gly Leu Ser Met His Lys Arg Glu Ile Ala Ile

1125 1130 1135

Lys Glu Ser Met Glu Asp Leu Val Thr Ala Pro Leu Pro Val Glu Asp

1140 1145 1150

Ala Leu Ile Ser Leu Phe Asp Cys Ser Asp Thr Thr Val Gln Gln Arg

1155 1160 1165

Val Ile Glu Thr Tyr Ile Ala Arg Leu Tyr Gln Pro His Leu Val Lys

1170 1175 1180

Asp Ser Ile Lys Met Lys Trp Ile Glu Ser Gly Val Ile Ala Leu Trp

1185 1190 1195 1200

Glu Phe Pro Glu Gly His Phe Asp Ala Arg Asn Gly Gly Ala Val Leu

1205 1210 1215

Gly Asp Lys Arg Trp Gly Ala Met Val Ile Val Lys Ser Leu Glu Ser

1220 1225 1230

Leu Ser Met Ala Ile Arg Phe Ala Leu Lys Glu Thr Ser His Tyr Thr

1235 1240 1245

Ser Ser Glu Gly Asn Met Met His Ile Ala Leu Leu Gly Ala Asp Asn

1250 1255 1260

Lys Met His Ile Ile Gln Glu Ser Gly Asp Asp Ala Asp Arg Ile Ala

1265 1270 1275 1280

Lys Leu Pro Leu Ile Leu Lys Asp Asn Val Thr Asp Leu His Ala Ser

1285 1290 1295

Gly Val Lys Thr Ile Ser Phe Ile Val Gln Arg Asp Glu Ala Arg Met

1300 1305 1310

Thr Met Arg Arg Thr Phe Leu Trp Ser Asp Glu Lys Leu Ser Tyr Glu

1315 1320 1325

Glu Glu Pro Ile Leu Arg His Val Glu Pro Pro Leu Ser Ala Leu Leu

1330 1335 1340

Glu Leu Asp Lys Leu Lys Val Lys Gly Tyr Asn Glu Met Lys Tyr Thr

1345 1350 1355 1360

Pro Ser Arg Asp Arg Gln Trp His Ile Tyr Thr Leu Arg Asn Thr Glu

1365 1370 1375

Asn Pro Lys Met Leu His Arg Val Phe Phe Arg Thr Leu Val Arg Gln

1380 1385 1390

Pro Ser Val Ser Asn Lys Phe Ser Ser Gly Gln Ile Gly Asp Met Glu

1395 1400 1405

Val Gly Ser Ala Glu Glu Pro Leu Ser Phe Thr Ser Thr Ser Ile Leu

1410 1415 1420

Arg Ser Leu Met Thr Ala Ile Glu Glu Leu Glu Leu His Ala Ile Arg

1425 1430 1435 1440

Thr Gly His Ser His Met Tyr Leu His Val Leu Lys Glu Gln Lys Leu

1445 1450 1455

Leu Asp Leu Val Pro Val Ser Gly Asn Thr Val Leu Asp Val Gly Gln

1460 1465 1470

Asp Glu Ala Thr Ala Tyr Ser Leu Leu Lys Glu Met Ala Met Lys Ile

1475 1480 1485

His Glu Leu Val Gly Ala Arg Met His His Leu Ser Val Cys Gln Trp

1490 1495 1500

Glu Val Lys Leu Lys Leu Asp Cys Asp Gly Pro Ala Ser Gly Thr Trp

1505 1510 1515 1520

Arg Ile Val Thr Thr Asn Val Thr Ser His Thr Cys Thr Val Asp Ile

1525 1530 1535

Tyr Arg Glu Met Glu Asp Lys Glu Ser Arg Lys Leu Val Tyr His Pro

1540 1545 1550

Ala Thr Pro Ala Ala Gly Pro Leu His Gly Val Ala Leu Asn Asn Pro

1555 1560 1565

Tyr Gln Pro Leu Ser Val Ile Asp Leu Lys Arg Cys Ser Ala Arg Asn

1570 1575 1580

Asn Arg Thr Thr Tyr Cys Tyr Asp Phe Pro Leu Ala Phe Glu Thr Ala

1585 1590 1595 1600

Val Arg Lys Ser Trp Ser Ser Ser Thr Ser Gly Ala Ser Lys Gly Val

1605 1610 1615

Glu Asn Ala Gln Cys Tyr Val Lys Ala Thr Glu Leu Val Phe Ala Asp

1620 1625 1630

Lys His Gly Ser Trp Gly Thr Pro Leu Val Gln Met Asp Arg Pro Ala

1635 1640 1645

Gly Leu Asn Asp Ile Gly Met Val Ala Trp Thr Leu Lys Met Ser Thr

1650 1655 1660

Pro Glu Phe Pro Ser Gly Arg Glu Ile Ile Val Val Ala Asn Asp Ile

1665 1670 1675 1680

Thr Phe Arg Ala Gly Ser Phe Gly Pro Arg Glu Asp Ala Phe Phe Glu

1685 1690 1695

Ala Val Thr Asn Leu Ala Cys Glu Lys Lys Leu Pro Leu Ile Tyr Leu

1700 1705 1710

Ala Ala Asn Ser Gly Ala Arg Ile Gly Ile Ala Asp Glu Val Lys Ser

1715 1720 1725

Cys Phe Arg Val Gly Trp Ser Asp Asp Gly Ser Pro Glu Arg Gly Phe

1730 1735 1740

Gln Tyr Ile Tyr Leu Ser Glu Glu Asp Tyr Ala Arg Ile Gly Thr Ser

1745 1750 1755 1760

Val Ile Ala His Lys Met Gln Leu Asp Ser Gly Glu Ile Arg Trp Val

1765 1770 1775

Ile Asp Ser Val Val Gly Lys Glu Asp Gly Leu Gly Val Glu Asn Ile

1780 1785 1790

His Gly Ser Ala Pro Ile Ala Ser Ala Tyr Ser Arg Ala Tyr Lys Glu

1795 1800 1805

Thr Phe Thr Leu Thr Phe Val Thr Gly Arg Thr Val Gly Ile Gly Ala

1810 1815 1820

Tyr Leu Ala Arg Leu Gly Ile Arg Cys Ile Gln Arg Leu Asp Gln Pro

1825 1830 1835 1840

Ile Ile Leu Thr Gly Tyr Ser Ala Leu Asn Lys Leu Leu Gly Arg Glu

1845 1850 1855

Val Tyr Ser Ser His Met Gln Leu Gly Gly Pro Lys Ile Met Ala Thr

1860 1865 1870

Asn Gly Val Val His Leu Thr Val Ser Asp Asp Leu Glu Gly Val Ser

1875 1880 1885

Asn Ile Leu Arg Trp Leu Ser Tyr Val Pro Ala Tyr Ile Gly Gly Pro

1890 1895 1900

Leu Pro Val Thr Thr Pro Leu Asp Pro Pro Asp Arg Pro Val Ala Tyr

1905 1910 1915 1920

Ile Pro Glu Asn Ser Cys Asp Pro Arg Ala Ala Ile Arg Gly Val Asp

1925 1930 1935

Asp Ser Gln Gly Lys Trp Leu Gly Gly Met Phe Asp Lys Asp Ser Phe

1940 1945 1950

Val Glu Thr Phe Glu Gly Trp Ala Lys Thr Val Val Thr Gly Arg Ala

1955 1960 1965

Lys Leu Gly Gly Ile Pro Val Gly Val Ile Ala Val Glu Thr Gln Thr

1970 1975 1980

Met Met Gln Thr Ile Pro Ala Asp Pro Gly Gln Leu Asp Ser Arg Glu

1985 1990 1995 2000

Gln Ser Val Pro Arg Ala Gly Gln Val Trp Phe Pro Asp Ser Ala Thr

2005 2010 2015

Lys Thr Ala Gln Ala Leu Leu Asp Phe Asn Arg Glu Gly Leu Pro Leu

2020 2025 2030

Phe Ile Leu Ala Asn Trp Arg Gly Phe Ser Gly Gly Gln Arg Asp Leu

2035 2040 2045

Phe Glu Gly Ile Leu Gln Ala Gly Ser Thr Ile Val Glu Asn Leu Arg

2050 2055 2060

Thr Tyr Asn Gln Pro Ala Phe Val Tyr Ile Pro Met Ala Ala Glu Leu

2065 2070 2075 2080

Arg Gly Gly Ala Trp Val Val Val Asp Ser Lys Ile Asn Pro Asp Arg

2085 2090 2095

Ile Glu Cys Tyr Ala Glu Arg Thr Ala Lys Gly Asn Val Leu Glu Pro

2100 2105 2110

Gln Gly Leu Ile Glu Ile Lys Phe Arg Ser Glu Glu Leu Gln Asp Cys

2115 2120 2125

Met Ser Arg Leu Asp Pro Thr Leu Ile Asp Leu Lys Ala Lys Leu Glu

2130 2135 2140

Val Ala Asn Lys Asn Gly Ser Ala Asp Thr Lys Ser Leu Gln Glu Asn

2145 2150 2155 2160

Ile Glu Ala Arg Thr Lys Gln Leu Met Pro Leu Tyr Thr Gln Ile Ala

2165 2170 2175

Ile Arg Phe Ala Glu Leu His Asp Thr Ser Leu Arg Met Ala Ala Lys

2180 2185 2190

Gly Val Ile Lys Lys Val Val Asp Trp Glu Glu Ser Arg Ser Phe Phe

2195 2200 2205

Tyr Lys Arg Leu Arg Arg Arg Ile Ser Glu Asp Val Leu Ala Lys Glu

2210 2215 2220

Ile Arg Ala Val Ala Gly Glu Gln Phe Ser His Gln Pro Ala Ile Glu

2225 2230 2235 2240

Leu Ile Lys Lys Trp Tyr Ser Ala Ser His Ala Ala Glu Trp Asp Asp

2245 2250 2255

Asp Asp Ala Phe Val Ala Trp Met Asp Asn Pro Glu Asn Tyr Lys Asp

2260 2265 2270

Tyr Ile Gln Tyr Leu Lys Ala Gln Arg Val Ser Gln Ser Leu Ser Ser

2275 2280 2285

Leu Ser Asp Ser Ser Ser Asp Leu Gln Ala Leu Pro Gln Gly Leu Ser

2290 2295 2300

Met Leu Leu Asp Lys Met Asp Pro Ser Arg Arg Ala Gln Leu Val Glu

2305 2310 2315 2320

Glu Ile Arg Lys Val Leu Gly

2325

<210> 5

<211> 6984

<212> DNA

<213> 水稻(Oryza sativa嘉花1号ACCase-5504突变核苷酸序列)

<400> 5

atgacatcca cacatgtggc gacattggga gttggtgccc aggcacctcc tcgtcaccag 60

aaaaagtcag ctggcactgc atttgtatca tctgggtcat caagaccctc ataccgaaag 120

aatggtcagc gtactcggtc acttagggaa gaaagcaatg gaggagtgtc tgattccaaa 180

aagcttaacc actctattcg ccaaggtctt gctggcatca ttgacctccc aaatgacgca 240

gcttcagaag ttgatatttc acatggttcc gaagatccca gggggcctac ggtcccaggt 300

tcctaccaaa tgaatgggat tatcaatgaa acacataatg ggaggcatgc ttcagtctcc 360

aaggttgttg agttttgtac ggcacttggt ggcaaaacac caattcacag tgtattagtg 420

gccaacaatg gaatggcagc agctaagttc atgcggagtg tccgaacatg ggctaatgat 480

acttttggat cagagaaggc aattcagctg atagctatgg caactccgga ggatctgagg 540

ataaatgcag agcacatcag aattgccgat caatttgtag aggtacctgg tggaacaaac 600

aacaacaact atgcaaatgt ccaactcata gtggagatag cagagagaac aggtgtttct 660

gctgtttggc ctggttgggg tcatgcatct gagaatcctg aacttccaga tgcgctgact 720

gcaaaaggaa ttgtttttct tgggccacca gcatcatcaa tgcatgcatt aggagacaag 780

gttggctcag ctctcattgc tcaagcagct ggagttccaa cacttgcttg gagtggatca 840

catgtggaag ttcctctgga gtgttgcttg gactcaatac ctgatgagat gtatagaaaa 900

gcttgtgtta ctaccacaga ggaagcagtt gcaagttgtc aggtggttgg ttatcctgcc 960

atgattaagg catcttgggg tggtggtggt aaaggaataa ggaaggttca taatgatgat 1020

gaggttagga cattatttaa gcaagttcaa ggcgaagtac ctggttcccc aatatttatc 1080

atgaggctag ctgctcagag tcgacatctt gaagttcagt tgctttgtga tcaatatggc 1140

aacgtagcag cacttcacag tcgagattgc agtgtacaac ggcgacacca aaagataatc 1200

gaggaaggac cagttactgt tgctcctcgt gagactgtga aagagcttga gcaggcagca 1260

cggaggcttg ctaaagctgt gggttatgtt ggtgctgcta ctgttgaata cctttacagc 1320

atggaaactg gtgaatatta ttttctggaa cttaatccac ggctacaggt tgagcatcct 1380

gtcactgagt ggatagctga agtaaatttg cctgcggctc aagttgctgt tggaatgggt 1440

ataccccttt ggcagattcc agagatcagg cgcttctacg gaatgaacca tggaggaggc 1500

tatgaccttt ggaggaaaac agcagctcta gcgactccat ttaactttga tgaagtagat 1560

tctaaatggc caaaaggcca ctgcgtagct gttagaataa ctagcgagga tccagatgat 1620

gggtttaagc ctactggtgg aaaagtaaag gagataagtt tcaagagtaa accaaatgtt 1680

tgggcctatt tctcagtaaa gtctggtgga ggcatccatg aattcgctga ttctcagttc 1740

ggacatgttt ttgcgtatgg aactactaga tcggcagcaa taactaccat ggctcttgca 1800

ctaaaagagg ttcaaattcg tggagaaatt cattcaaacg tagactacac agttgaccta 1860

ttaaatgcct cagattttag agaaaataag attcatactg gttggctgga taccaggata 1920

gccatgcgtg ttcaagctga gaggcctcca tggtatattt cagtcgttgg aggggcttta 1980

tataaaacag taactgccaa cacggccact gtttctgatt atgttggtta tcttaccaag 2040

ggccagattc caccaaagca tatatccctt gtctatacga ctgttgcttt gaatatagat 2100

gggaaaaaat atacaatcga tactgtgagg agtggacatg gtagctacag attgcgaatg 2160

aatggatcaa cggttgacgc aaatgtacaa atattatgtg atggtgggct tttaatgcag 2220

ctggatggaa acagccatgt aatttatgct gaagaagagg ccagtggtac acgacttctt 2280

attgatggaa agacatgcat gttacagaat gaccatgacc catcaaagtt attagctgag 2340

acaccatgca aacttcttcg tttcttggtt gctgatggtg ctcatgttga tgctgatgta 2400

ccatatgcgg aagttgaggt tatgaagatg tgcatgcccc tcttatcacc cgcttctggt 2460

gtcatacatg ttgtaatgtc tgagggccaa gcaatgcagg ctggtgatct tatagctagg 2520

ctggatcttg atgacccttc tgctgttaag agagctgagc cgttcgaaga tacttttcca 2580

caaatgggtc tccctattgc tgcttctggc caagttcaca aattatgtgc tgcaagtctg 2640

aatgcttgtc gaatgatcct tgcggggtat gagcatgata ttgacaaggt tgtgccagag 2700

ttggtatact gcctagacac tccggagctt cctttcctgc agtgggagga gcttatgtct 2760

gttttagcaa ctagacttcc aagaaatctt aaaagtgagt tggagggcaa atatgaggaa 2820

tacaaagtaa aatttgactc tgggataatc aatgatttcc ctgccaatat gctacgagtg 2880

ataattgagg aaaatcttgc atgtggttct gagaaggaga aggctacaaa tgagaggctt 2940

gttgagcctc ttatgagcct actgaagtca tatgagggtg ggagagaaag tcatgctcac 3000

tttgttgtca agtccctttt tgaggagtat ctctatgttg aagaattgtt cagtgatgga 3060

attcagtctg atgtgattga gcgtctgcgc cttcaacata gtaaagacct acagaaggtc 3120

gtagacattg tgttgtccca ccagagtgtt agaaataaaa ctaagctgat actaaaactc 3180

atggagagtc tggtctatcc aaatcctgct gcctacaggg atcaattgat tcgcttttct 3240

tcccttaatc acaaagcgta ttacaagttg gcacttaaag ctagtgaact tcttgaacaa 3300

acaaaactta gtgagctccg tgcaagaata gcaaggagcc tttcagagct ggagatgttt 3360

actgaggaaa gcaagggtct ctccatgcat aagcgagaaa ttgccattaa ggagagcatg 3420

gaagatttag tcactgctcc actgccagtt gaagatgcgc tcatttcttt atttgattgt 3480

agtgatacaa ctgttcaaca gagagtgatt gagacttata tagctcgatt ataccagcct 3540

catcttgtaa aggacagtat caaaatgaaa tggatagaat cgggtgttat tgctttatgg 3600

gaatttcctg aagggcattt tgatgcaaga aatggaggag cggttcttgg tgacaaaaga 3660

tggggtgcca tggtcattgt caagtctctt gaatcacttt caatggccat tagatttgca 3720

ctaaaggaga catcacacta cactagctct gagggcaata tgatgcatat tgctttgttg 3780

ggtgctgata ataagatgca tataattcaa gaaagtggtg atgatgctga cagaatagcc 3840

aaacttccct tgatactaaa ggataatgta accgatctgc atgcctctgg tgtgaaaaca 3900

ataagtttca ttgttcaaag agatgaagca cggatgacaa tgcgtcgtac cttcctttgg 3960

tctgatgaaa agctttctta tgaggaagag ccaattctcc ggcatgtgga acctcctctt 4020

tctgcacttc ttgagttgga caagttgaaa gtgaaaggat acaatgaaat gaagtatacc 4080

ccatcacggg atcgtcaatg gcatatctac acacttagaa atactgaaaa ccccaaaatg 4140

ttgcaccggg tatttttccg aacccttgtc aggcaaccca gtgtatccaa caagttttct 4200

tcgggccaga ttggtgacat ggaagttggg agtgctgaag aacctctgtc atttacatca 4260

accagcatat taagatcttt gatgactgct atagaggaat tggagcttca cgcaattaga 4320

actggccatt cacacatgta tttgcatgta ttgaaagaac aaaagcttct tgatcttgtt 4380

ccagtttcag ggaatacagt tttggatgtt ggtcaagatg aagctactgc atattcactt 4440

ttaaaagaaa tggctatgaa gatacatgaa cttgttggtg caagaatgca ccatctttct 4500

gtatgccaat gggaagtgaa acttaagttg gactgcgatg gtcctgccag tggtacctgg 4560

aggattgtaa caaccaatgt tactagtcac acttgcactg tggatatcta ccgtgagatg 4620

gaagataaag aatcacggaa gttagtatac catcccgcca ctccggcggc tggtcctctg 4680

catggtgtgg cactgaataa tccatatcag cctttgagtg tcattgatct caaacgctgt 4740

tctgctagga ataatagaac tacatactgc tatgattttc cactggcatt tgaaactgca 4800

gtgaggaagt catggtcctc tagtacctct ggtgcttcta aaggtgttga aaatgcccaa 4860

tgttatgtta aagctacaga gttggtattt gcggacaaac atgggtcatg gggcactcct 4920

ttagttcaaa tggaccggcc tgctgggctc aatgacattg gtatggtagc ttggaccttg 4980

aagatgtcca ctcctgaatt tcctagtggt agggagatta ttgttgttgc aaatgatatt 5040

acgttcagag ctggatcatt tggcccaagg gaagatgcat tttttgaagc tgttaccaac 5100

ctagcctgtg agaagaaact tcctcttatt tatttggcag caaattctgg tgctcgaatt 5160

ggcatagcag atgaagtgaa atcttgcttc cgtgttgggt ggtctgatga tggcagccct 5220

gaacgtgggt ttcagtacat ttatctaagc gaagaagact atgctcgtat tggcacttct 5280

gtcatagcac ataagatgca gctagacagt ggtgaaatta ggtgggttat tgattctgtt 5340

gtgggcaagg aagatggact tggtgtggag aatatacatg gaagtgctgc tattgccagt 5400

gcttattcta gggcatataa ggagacattt acacttacat ttgtgactgg aagaactgtt 5460

ggaataggag cttatcttgc tcgacttggc atccggtgca taccgcgtct tgaccagcct 5520

attattctta caggctattc tgcactgaac aagcttcttg ggcgggaagt gtacagctcc 5580

cacatgcagt tgggtggtcc caaaatcatg gcaactaatg gtgttgtcca tcttactgtt 5640

tcagatgacc ttgaaggcgt ttctaatata ttgaggtggc tcagttatgt tcctgcctac 5700

attggtggac cacttccagt aacaacaccg ttggacccac cggacagacc tgttgcatac 5760

attcctgaga actcgtgtga tcctcgagcg gctatccgtg gtgttgatga cagccaaggg 5820

aaatggttag gtggtatgtt tgataaagac agctttgtgg aaacatttga aggttgggct 5880

aagacagtgg ttactggcag agcaaagctt ggtggaattc cagtgggtgt gatagctgtg 5940

gagactcaga ccatgatgca aactatccct gctgaccctg gtcagcttga ttcccgtgag 6000

caatctgttc ctcgtgctgg acaagtgtgg tttccagatt ctgcaaccaa gactgcgcag 6060

gcattgctgg acttcaaccg tgaaggatta cctctgttca tcctcgctaa ctggagaggc 6120

ttctctggtg gacaaagaga tctttttgaa ggaattcttc aggctggctc gactattgtt 6180

gagaacctta ggacatacaa tcagcctgcc tttgtctaca ttcccatggc tgcagagcta 6240

cgaggagggg cttgggttgt ggttgatagc aagataaacc cagaccgcat tgagtgctat 6300

gctgagagga ctgcaaaagg caatgttctg gaaccgcaag ggttaattga gatcaagttc 6360

aggtcagagg aactccagga ttgcatgagt cggcttgacc caacattaat tgatctgaaa 6420

gcaaaactcg aagtagcaaa taaaaatgga agtgctgaca caaaatcgct tcaagaaaat 6480

atagaagctc gaacaaaaca gttgatgcct ctatatactc agattgcgat acggtttgct 6540

gaattgcatg atacatccct cagaatggct gcgaaaggtg tgattaagaa agttgtggac 6600

tgggaagaat cacgatcttt cttctataag agattacgga ggaggatctc tgaggatgtt 6660

cttgcaaaag aaattagagc tgtagcaggt gagcagtttt cccaccaacc agcaatcgag 6720

ctgatcaaga aatggtattc agcttcacat gcagctgaat gggatgatga cgatgctttt 6780

gttgcttgga tggataaccc tgaaaactac aaggattata ttcaatatct taaggctcaa 6840

agagtatccc aatccctctc aagtctttca gattccagct cagatttgca agccctgcca 6900

cagggtcttt ccatgttact agataagatg gatccctcta gaagagctca acttgttgaa 6960

gaaatcagga aggtccttgg ttga 6984

<210> 6

<211> 2327

<212> PRT

<213> 水稻(Oryza sativa嘉花1号ACCase-5504突变氨基酸序列)

<400> 6

Met Thr Ser Thr His Val Ala Thr Leu Gly Val Gly Ala Gln Ala Pro

1 5 10 15

Pro Arg His Gln Lys Lys Ser Ala Gly Thr Ala Phe Val Ser Ser Gly

20 25 30

Ser Ser Arg Pro Ser Tyr Arg Lys Asn Gly Gln Arg Thr Arg Ser Leu

35 40 45

Arg Glu Glu Ser Asn Gly Gly Val Ser Asp Ser Lys Lys Leu Asn His

50 55 60

Ser Ile Arg Gln Gly Leu Ala Gly Ile Ile Asp Leu Pro Asn Asp Ala

65 70 75 80

Ala Ser Glu Val Asp Ile Ser His Gly Ser Glu Asp Pro Arg Gly Pro

85 90 95

Thr Val Pro Gly Ser Tyr Gln Met Asn Gly Ile Ile Asn Glu Thr His

100 105 110

Asn Gly Arg His Ala Ser Val Ser Lys Val Val Glu Phe Cys Thr Ala

115 120 125

Leu Gly Gly Lys Thr Pro Ile His Ser Val Leu Val Ala Asn Asn Gly

130 135 140

Met Ala Ala Ala Lys Phe Met Arg Ser Val Arg Thr Trp Ala Asn Asp

145 150 155 160

Thr Phe Gly Ser Glu Lys Ala Ile Gln Leu Ile Ala Met Ala Thr Pro

165 170 175

Glu Asp Leu Arg Ile Asn Ala Glu His Ile Arg Ile Ala Asp Gln Phe

180 185 190

Val Glu Val Pro Gly Gly Thr Asn Asn Asn Asn Tyr Ala Asn Val Gln

195 200 205

Leu Ile Val Glu Ile Ala Glu Arg Thr Gly Val Ser Ala Val Trp Pro

210 215 220

Gly Trp Gly His Ala Ser Glu Asn Pro Glu Leu Pro Asp Ala Leu Thr

225 230 235 240

Ala Lys Gly Ile Val Phe Leu Gly Pro Pro Ala Ser Ser Met His Ala

245 250 255

Leu Gly Asp Lys Val Gly Ser Ala Leu Ile Ala Gln Ala Ala Gly Val

260 265 270

Pro Thr Leu Ala Trp Ser Gly Ser His Val Glu Val Pro Leu Glu Cys

275 280 285

Cys Leu Asp Ser Ile Pro Asp Glu Met Tyr Arg Lys Ala Cys Val Thr

290 295 300

Thr Thr Glu Glu Ala Val Ala Ser Cys Gln Val Val Gly Tyr Pro Ala

305 310 315 320

Met Ile Lys Ala Ser Trp Gly Gly Gly Gly Lys Gly Ile Arg Lys Val

325 330 335

His Asn Asp Asp Glu Val Arg Thr Leu Phe Lys Gln Val Gln Gly Glu

340 345 350

Val Pro Gly Ser Pro Ile Phe Ile Met Arg Leu Ala Ala Gln Ser Arg

355 360 365

His Leu Glu Val Gln Leu Leu Cys Asp Gln Tyr Gly Asn Val Ala Ala

370 375 380

Leu His Ser Arg Asp Cys Ser Val Gln Arg Arg His Gln Lys Ile Ile

385 390 395 400

Glu Glu Gly Pro Val Thr Val Ala Pro Arg Glu Thr Val Lys Glu Leu

405 410 415

Glu Gln Ala Ala Arg Arg Leu Ala Lys Ala Val Gly Tyr Val Gly Ala

420 425 430

Ala Thr Val Glu Tyr Leu Tyr Ser Met Glu Thr Gly Glu Tyr Tyr Phe

435 440 445

Leu Glu Leu Asn Pro Arg Leu Gln Val Glu His Pro Val Thr Glu Trp

450 455 460

Ile Ala Glu Val Asn Leu Pro Ala Ala Gln Val Ala Val Gly Met Gly

465 470 475 480

Ile Pro Leu Trp Gln Ile Pro Glu Ile Arg Arg Phe Tyr Gly Met Asn

485 490 495

His Gly Gly Gly Tyr Asp Leu Trp Arg Lys Thr Ala Ala Leu Ala Thr

500 505 510

Pro Phe Asn Phe Asp Glu Val Asp Ser Lys Trp Pro Lys Gly His Cys

515 520 525

Val Ala Val Arg Ile Thr Ser Glu Asp Pro Asp Asp Gly Phe Lys Pro

530 535 540

Thr Gly Gly Lys Val Lys Glu Ile Ser Phe Lys Ser Lys Pro Asn Val

545 550 555 560

Trp Ala Tyr Phe Ser Val Lys Ser Gly Gly Gly Ile His Glu Phe Ala

565 570 575

Asp Ser Gln Phe Gly His Val Phe Ala Tyr Gly Thr Thr Arg Ser Ala

580 585 590

Ala Ile Thr Thr Met Ala Leu Ala Leu Lys Glu Val Gln Ile Arg Gly

595 600 605

Glu Ile His Ser Asn Val Asp Tyr Thr Val Asp Leu Leu Asn Ala Ser

610 615 620

Asp Phe Arg Glu Asn Lys Ile His Thr Gly Trp Leu Asp Thr Arg Ile

625 630 635 640

Ala Met Arg Val Gln Ala Glu Arg Pro Pro Trp Tyr Ile Ser Val Val

645 650 655

Gly Gly Ala Leu Tyr Lys Thr Val Thr Ala Asn Thr Ala Thr Val Ser

660 665 670

Asp Tyr Val Gly Tyr Leu Thr Lys Gly Gln Ile Pro Pro Lys His Ile

675 680 685

Ser Leu Val Tyr Thr Thr Val Ala Leu Asn Ile Asp Gly Lys Lys Tyr

690 695 700

Thr Ile Asp Thr Val Arg Ser Gly His Gly Ser Tyr Arg Leu Arg Met

705 710 715 720

Asn Gly Ser Thr Val Asp Ala Asn Val Gln Ile Leu Cys Asp Gly Gly

725 730 735

Leu Leu Met Gln Leu Asp Gly Asn Ser His Val Ile Tyr Ala Glu Glu

740 745 750

Glu Ala Ser Gly Thr Arg Leu Leu Ile Asp Gly Lys Thr Cys Met Leu

755 760 765

Gln Asn Asp His Asp Pro Ser Lys Leu Leu Ala Glu Thr Pro Cys Lys

770 775 780

Leu Leu Arg Phe Leu Val Ala Asp Gly Ala His Val Asp Ala Asp Val

785 790 795 800

Pro Tyr Ala Glu Val Glu Val Met Lys Met Cys Met Pro Leu Leu Ser

805 810 815

Pro Ala Ser Gly Val Ile His Val Val Met Ser Glu Gly Gln Ala Met

820 825 830

Gln Ala Gly Asp Leu Ile Ala Arg Leu Asp Leu Asp Asp Pro Ser Ala

835 840 845

Val Lys Arg Ala Glu Pro Phe Glu Asp Thr Phe Pro Gln Met Gly Leu

850 855 860

Pro Ile Ala Ala Ser Gly Gln Val His Lys Leu Cys Ala Ala Ser Leu

865 870 875 880

Asn Ala Cys Arg Met Ile Leu Ala Gly Tyr Glu His Asp Ile Asp Lys

885 890 895

Val Val Pro Glu Leu Val Tyr Cys Leu Asp Thr Pro Glu Leu Pro Phe

900 905 910

Leu Gln Trp Glu Glu Leu Met Ser Val Leu Ala Thr Arg Leu Pro Arg

915 920 925

Asn Leu Lys Ser Glu Leu Glu Gly Lys Tyr Glu Glu Tyr Lys Val Lys

930 935 940

Phe Asp Ser Gly Ile Ile Asn Asp Phe Pro Ala Asn Met Leu Arg Val

945 950 955 960

Ile Ile Glu Glu Asn Leu Ala Cys Gly Ser Glu Lys Glu Lys Ala Thr

965 970 975

Asn Glu Arg Leu Val Glu Pro Leu Met Ser Leu Leu Lys Ser Tyr Glu

980 985 990

Gly Gly Arg Glu Ser His Ala His Phe Val Val Lys Ser Leu Phe Glu

995 1000 1005

Glu Tyr Leu Tyr Val Glu Glu Leu Phe Ser Asp Gly Ile Gln Ser Asp

1010 1015 1020

Val Ile Glu Arg Leu Arg Leu Gln His Ser Lys Asp Leu Gln Lys Val

1025 1030 1035 1040

Val Asp Ile Val Leu Ser His Gln Ser Val Arg Asn Lys Thr Lys Leu

1045 1050 1055

Ile Leu Lys Leu Met Glu Ser Leu Val Tyr Pro Asn Pro Ala Ala Tyr

1060 1065 1070

Arg Asp Gln Leu Ile Arg Phe Ser Ser Leu Asn His Lys Ala Tyr Tyr

1075 1080 1085

Lys Leu Ala Leu Lys Ala Ser Glu Leu Leu Glu Gln Thr Lys Leu Ser

1090 1095 1100

Glu Leu Arg Ala Arg Ile Ala Arg Ser Leu Ser Glu Leu Glu Met Phe

1105 1110 1115 1120

Thr Glu Glu Ser Lys Gly Leu Ser Met His Lys Arg Glu Ile Ala Ile

1125 1130 1135

Lys Glu Ser Met Glu Asp Leu Val Thr Ala Pro Leu Pro Val Glu Asp

1140 1145 1150

Ala Leu Ile Ser Leu Phe Asp Cys Ser Asp Thr Thr Val Gln Gln Arg

1155 1160 1165

Val Ile Glu Thr Tyr Ile Ala Arg Leu Tyr Gln Pro His Leu Val Lys

1170 1175 1180

Asp Ser Ile Lys Met Lys Trp Ile Glu Ser Gly Val Ile Ala Leu Trp

1185 1190 1195 1200

Glu Phe Pro Glu Gly His Phe Asp Ala Arg Asn Gly Gly Ala Val Leu

1205 1210 1215

Gly Asp Lys Arg Trp Gly Ala Met Val Ile Val Lys Ser Leu Glu Ser

1220 1225 1230

Leu Ser Met Ala Ile Arg Phe Ala Leu Lys Glu Thr Ser His Tyr Thr

1235 1240 1245

Ser Ser Glu Gly Asn Met Met His Ile Ala Leu Leu Gly Ala Asp Asn

1250 1255 1260

Lys Met His Ile Ile Gln Glu Ser Gly Asp Asp Ala Asp Arg Ile Ala

1265 1270 1275 1280

Lys Leu Pro Leu Ile Leu Lys Asp Asn Val Thr Asp Leu His Ala Ser

1285 1290 1295

Gly Val Lys Thr Ile Ser Phe Ile Val Gln Arg Asp Glu Ala Arg Met

1300 1305 1310

Thr Met Arg Arg Thr Phe Leu Trp Ser Asp Glu Lys Leu Ser Tyr Glu

1315 1320 1325

Glu Glu Pro Ile Leu Arg His Val Glu Pro Pro Leu Ser Ala Leu Leu

1330 1335 1340

Glu Leu Asp Lys Leu Lys Val Lys Gly Tyr Asn Glu Met Lys Tyr Thr

1345 1350 1355 1360

Pro Ser Arg Asp Arg Gln Trp His Ile Tyr Thr Leu Arg Asn Thr Glu

1365 1370 1375

Asn Pro Lys Met Leu His Arg Val Phe Phe Arg Thr Leu Val Arg Gln

1380 1385 1390

Pro Ser Val Ser Asn Lys Phe Ser Ser Gly Gln Ile Gly Asp Met Glu

1395 1400 1405

Val Gly Ser Ala Glu Glu Pro Leu Ser Phe Thr Ser Thr Ser Ile Leu

1410 1415 1420

Arg Ser Leu Met Thr Ala Ile Glu Glu Leu Glu Leu His Ala Ile Arg

1425 1430 1435 1440

Thr Gly His Ser His Met Tyr Leu His Val Leu Lys Glu Gln Lys Leu

1445 1450 1455

Leu Asp Leu Val Pro Val Ser Gly Asn Thr Val Leu Asp Val Gly Gln

1460 1465 1470

Asp Glu Ala Thr Ala Tyr Ser Leu Leu Lys Glu Met Ala Met Lys Ile

1475 1480 1485

His Glu Leu Val Gly Ala Arg Met His His Leu Ser Val Cys Gln Trp

1490 1495 1500

Glu Val Lys Leu Lys Leu Asp Cys Asp Gly Pro Ala Ser Gly Thr Trp

1505 1510 1515 1520

Arg Ile Val Thr Thr Asn Val Thr Ser His Thr Cys Thr Val Asp Ile

1525 1530 1535

Tyr Arg Glu Met Glu Asp Lys Glu Ser Arg Lys Leu Val Tyr His Pro

1540 1545 1550

Ala Thr Pro Ala Ala Gly Pro Leu His Gly Val Ala Leu Asn Asn Pro

1555 1560 1565

Tyr Gln Pro Leu Ser Val Ile Asp Leu Lys Arg Cys Ser Ala Arg Asn

1570 1575 1580

Asn Arg Thr Thr Tyr Cys Tyr Asp Phe Pro Leu Ala Phe Glu Thr Ala

1585 1590 1595 1600

Val Arg Lys Ser Trp Ser Ser Ser Thr Ser Gly Ala Ser Lys Gly Val

1605 1610 1615

Glu Asn Ala Gln Cys Tyr Val Lys Ala Thr Glu Leu Val Phe Ala Asp

1620 1625 1630

Lys His Gly Ser Trp Gly Thr Pro Leu Val Gln Met Asp Arg Pro Ala

1635 1640 1645

Gly Leu Asn Asp Ile Gly Met Val Ala Trp Thr Leu Lys Met Ser Thr

1650 1655 1660

Pro Glu Phe Pro Ser Gly Arg Glu Ile Ile Val Val Ala Asn Asp Ile

1665 1670 1675 1680

Thr Phe Arg Ala Gly Ser Phe Gly Pro Arg Glu Asp Ala Phe Phe Glu

1685 1690 1695

Ala Val Thr Asn Leu Ala Cys Glu Lys Lys Leu Pro Leu Ile Tyr Leu

1700 1705 1710

Ala Ala Asn Ser Gly Ala Arg Ile Gly Ile Ala Asp Glu Val Lys Ser

1715 1720 1725

Cys Phe Arg Val Gly Trp Ser Asp Asp Gly Ser Pro Glu Arg Gly Phe

1730 1735 1740

Gln Tyr Ile Tyr Leu Ser Glu Glu Asp Tyr Ala Arg Ile Gly Thr Ser

1745 1750 1755 1760

Val Ile Ala His Lys Met Gln Leu Asp Ser Gly Glu Ile Arg Trp Val

1765 1770 1775

Ile Asp Ser Val Val Gly Lys Glu Asp Gly Leu Gly Val Glu Asn Ile

1780 1785 1790

His Gly Ser Ala Ala Ile Ala Ser Ala Tyr Ser Arg Ala Tyr Lys Glu

1795 1800 1805

Thr Phe Thr Leu Thr Phe Val Thr Gly Arg Thr Val Gly Ile Gly Ala

1810 1815 1820

Tyr Leu Ala Arg Leu Gly Ile Arg Cys Ile Asp Arg Leu Asp Gln Pro

1825 1830 1835 1840

Ile Ile Leu Thr Gly Tyr Ser Ala Leu Asn Lys Leu Leu Gly Arg Glu

1845 1850 1855

Val Tyr Ser Ser His Met Gln Leu Gly Gly Pro Lys Ile Met Ala Thr

1860 1865 1870

Asn Gly Val Val His Leu Thr Val Ser Asp Asp Leu Glu Gly Val Ser

1875 1880 1885

Asn Ile Leu Arg Trp Leu Ser Tyr Val Pro Ala Tyr Ile Gly Gly Pro

1890 1895 1900

Leu Pro Val Thr Thr Pro Leu Asp Pro Pro Asp Arg Pro Val Ala Tyr

1905 1910 1915 1920

Ile Pro Glu Asn Ser Cys Asp Pro Arg Ala Ala Ile Arg Gly Val Asp

1925 1930 1935

Asp Ser Gln Gly Lys Trp Leu Gly Gly Met Phe Asp Lys Asp Ser Phe

1940 1945 1950

Val Glu Thr Phe Glu Gly Trp Ala Lys Thr Val Val Thr Gly Arg Ala

1955 1960 1965

Lys Leu Gly Gly Ile Pro Val Gly Val Ile Ala Val Glu Thr Gln Thr

1970 1975 1980

Met Met Gln Thr Ile Pro Ala Asp Pro Gly Gln Leu Asp Ser Arg Glu

1985 1990 1995 2000

Gln Ser Val Pro Arg Ala Gly Gln Val Trp Phe Pro Asp Ser Ala Thr

2005 2010 2015

Lys Thr Ala Gln Ala Leu Leu Asp Phe Asn Arg Glu Gly Leu Pro Leu

2020 2025 2030

Phe Ile Leu Ala Asn Trp Arg Gly Phe Ser Gly Gly Gln Arg Asp Leu

2035 2040 2045

Phe Glu Gly Ile Leu Gln Ala Gly Ser Thr Ile Val Glu Asn Leu Arg

2050 2055 2060

Thr Tyr Asn Gln Pro Ala Phe Val Tyr Ile Pro Met Ala Ala Glu Leu

2065 2070 2075 2080

Arg Gly Gly Ala Trp Val Val Val Asp Ser Lys Ile Asn Pro Asp Arg

2085 2090 2095

Ile Glu Cys Tyr Ala Glu Arg Thr Ala Lys Gly Asn Val Leu Glu Pro

2100 2105 2110

Gln Gly Leu Ile Glu Ile Lys Phe Arg Ser Glu Glu Leu Gln Asp Cys

2115 2120 2125

Met Ser Arg Leu Asp Pro Thr Leu Ile Asp Leu Lys Ala Lys Leu Glu

2130 2135 2140

Val Ala Asn Lys Asn Gly Ser Ala Asp Thr Lys Ser Leu Gln Glu Asn

2145 2150 2155 2160

Ile Glu Ala Arg Thr Lys Gln Leu Met Pro Leu Tyr Thr Gln Ile Ala

2165 2170 2175

Ile Arg Phe Ala Glu Leu His Asp Thr Ser Leu Arg Met Ala Ala Lys

2180 2185 2190

Gly Val Ile Lys Lys Val Val Asp Trp Glu Glu Ser Arg Ser Phe Phe

2195 2200 2205

Tyr Lys Arg Leu Arg Arg Arg Ile Ser Glu Asp Val Leu Ala Lys Glu

2210 2215 2220

Ile Arg Ala Val Ala Gly Glu Gln Phe Ser His Gln Pro Ala Ile Glu

2225 2230 2235 2240

Leu Ile Lys Lys Trp Tyr Ser Ala Ser His Ala Ala Glu Trp Asp Asp

2245 2250 2255

Asp Asp Ala Phe Val Ala Trp Met Asp Asn Pro Glu Asn Tyr Lys Asp

2260 2265 2270

Tyr Ile Gln Tyr Leu Lys Ala Gln Arg Val Ser Gln Ser Leu Ser Ser

2275 2280 2285

Leu Ser Asp Ser Ser Ser Asp Leu Gln Ala Leu Pro Gln Gly Leu Ser

2290 2295 2300

Met Leu Leu Asp Lys Met Asp Pro Ser Arg Arg Ala Gln Leu Val Glu

2305 2310 2315 2320

Glu Ile Arg Lys Val Leu Gly

2325

<210> 7

<211> 6984

<212> DNA

<213> 水稻(Oryza sativa嘉花1号ACCase-5623突变核苷酸序列)

<400> 7

atgacatcca cacatgtggc gacattggga gttggtgccc aggcacctcc tcgtcaccag 60

aaaaagtcag ctggcactgc atttgtatca tctgggtcat caagaccctc ataccgaaag 120

aatggtcagc gtactcggtc acttagggaa gaaagcaatg gaggagtgtc tgattccaaa 180

aagcttaacc actctattcg ccaaggtctt gctggcatca ttgacctccc aaatgacgca 240

gcttcagaag ttgatatttc acatggttcc gaagatccca gggggcctac ggtcccaggt 300

tcctaccaaa tgaatgggat tatcaatgaa acacataatg ggaggcatgc ttcagtctcc 360

aaggttgttg agttttgtac ggcacttggt ggcaaaacac caattcacag tgtattagtg 420

gccaacaatg gaatggcagc agctaagttc atgcggagtg tccgaacatg ggctaatgat 480

acttttggat cagagaaggc aattcagctg atagctatgg caactccgga ggatctgagg 540

ataaatgcag agcacatcag aattgccgat caatttgtag aggtacctgg tggaacaaac 600

aacaacaact atgcaaatgt ccaactcata gtggagatag cagagagaac aggtgtttct 660

gctgtttggc ctggttgggg tcatgcatct gagaatcctg aacttccaga tgcgctgact 720

gcaaaaggaa ttgtttttct tgggccacca gcatcatcaa tgcatgcatt aggagacaag 780

gttggctcag ctctcattgc tcaagcagct ggagttccaa cacttgcttg gagtggatca 840

catgtggaag ttcctctgga gtgttgcttg gactcaatac ctgatgagat gtatagaaaa 900

gcttgtgtta ctaccacaga ggaagcagtt gcaagttgtc aggtggttgg ttatcctgcc 960

atgattaagg catcttgggg tggtggtggt aaaggaataa ggaaggttca taatgatgat 1020

gaggttagga cattatttaa gcaagttcaa ggcgaagtac ctggttcccc aatatttatc 1080

atgaggctag ctgctcagag tcgacatctt gaagttcagt tgctttgtga tcaatatggc 1140

aacgtagcag cacttcacag tcgagattgc agtgtacaac ggcgacacca aaagataatc 1200

gaggaaggac cagttactgt tgctcctcgt gagactgtga aagagcttga gcaggcagca 1260

cggaggcttg ctaaagctgt gggttatgtt ggtgctgcta ctgttgaata cctttacagc 1320

atggaaactg gtgaatatta ttttctggaa cttaatccac ggctacaggt tgagcatcct 1380

gtcactgagt ggatagctga agtaaatttg cctgcggctc aagttgctgt tggaatgggt 1440

ataccccttt ggcagattcc agagatcagg cgcttctacg gaatgaacca tggaggaggc 1500

tatgaccttt ggaggaaaac agcagctcta gcgactccat ttaactttga tgaagtagat 1560

tctaaatggc caaaaggcca ctgcgtagct gttagaataa ctagcgagga tccagatgat 1620

gggtttaagc ctactggtgg aaaagtaaag gagataagtt tcaagagtaa accaaatgtt 1680

tgggcctatt tctcagtaaa gtctggtgga ggcatccatg aattcgctga ttctcagttc 1740

ggacatgttt ttgcgtatgg aactactaga tcggcagcaa taactaccat ggctcttgca 1800

ctaaaagagg ttcaaattcg tggagaaatt cattcaaacg tagactacac agttgaccta 1860

ttaaatgcct cagattttag agaaaataag attcatactg gttggctgga taccaggata 1920

gccatgcgtg ttcaagctga gaggcctcca tggtatattt cagtcgttgg aggggcttta 1980

tataaaacag taactgccaa cacggccact gtttctgatt atgttggtta tcttaccaag 2040

ggccagattc caccaaagca tatatccctt gtctatacga ctgttgcttt gaatatagat 2100

gggaaaaaat atacaatcga tactgtgagg agtggacatg gtagctacag attgcgaatg 2160

aatggatcaa cggttgacgc aaatgtacaa atattatgtg atggtgggct tttaatgcag 2220

ctggatggaa acagccatgt aatttatgct gaagaagagg ccagtggtac acgacttctt 2280

attgatggaa agacatgcat gttacagaat gaccatgacc catcaaagtt attagctgag 2340

acaccatgca aacttcttcg tttcttggtt gctgatggtg ctcatgttga tgctgatgta 2400

ccatatgcgg aagttgaggt tatgaagatg tgcatgcccc tcttatcacc cgcttctggt 2460

gtcatacatg ttgtaatgtc tgagggccaa gcaatgcagg ctggtgatct tatagctagg 2520

ctggatcttg atgacccttc tgctgttaag agagctgagc cgttcgaaga tacttttcca 2580

caaatgggtc tccctattgc tgcttctggc caagttcaca aattatgtgc tgcaagtctg 2640

aatgcttgtc gaatgatcct tgcggggtat gagcatgata ttgacaaggt tgtgccagag 2700

ttggtatact gcctagacac tccggagctt cctttcctgc agtgggagga gcttatgtct 2760

gttttagcaa ctagacttcc aagaaatctt aaaagtgagt tggagggcaa atatgaggaa 2820

tacaaagtaa aatttgactc tgggataatc aatgatttcc ctgccaatat gctacgagtg 2880

ataattgagg aaaatcttgc atgtggttct gagaaggaga aggctacaaa tgagaggctt 2940

gttgagcctc ttatgagcct actgaagtca tatgagggtg ggagagaaag tcatgctcac 3000

tttgttgtca agtccctttt tgaggagtat ctctatgttg aagaattgtt cagtgatgga 3060

attcagtctg atgtgattga gcgtctgcgc cttcaacata gtaaagacct acagaaggtc 3120

gtagacattg tgttgtccca ccagagtgtt agaaataaaa ctaagctgat actaaaactc 3180

atggagagtc tggtctatcc aaatcctgct gcctacaggg atcaattgat tcgcttttct 3240

tcccttaatc acaaagcgta ttacaagttg gcacttaaag ctagtgaact tcttgaacaa 3300

acaaaactta gtgagctccg tgcaagaata gcaaggagcc tttcagagct ggagatgttt 3360

actgaggaaa gcaagggtct ctccatgcat aagcgagaaa ttgccattaa ggagagcatg 3420

gaagatttag tcactgctcc actgccagtt gaagatgcgc tcatttcttt atttgattgt 3480

agtgatacaa ctgttcaaca gagagtgatt gagacttata tagctcgatt ataccagcct 3540

catcttgtaa aggacagtat caaaatgaaa tggatagaat cgggtgttat tgctttatgg 3600

gaatttcctg aagggcattt tgatgcaaga aatggaggag cggttcttgg tgacaaaaga 3660

tggggtgcca tggtcattgt caagtctctt gaatcacttt caatggccat tagatttgca 3720

ctaaaggaga catcacacta cactagctct gagggcaata tgatgcatat tgctttgttg 3780

ggtgctgata ataagatgca tataattcaa gaaagtggtg atgatgctga cagaatagcc 3840

aaacttccct tgatactaaa ggataatgta accgatctgc atgcctctgg tgtgaaaaca 3900

ataagtttca ttgttcaaag agatgaagca cggatgacaa tgcgtcgtac cttcctttgg 3960

tctgatgaaa agctttctta tgaggaagag ccaattctcc ggcatgtgga acctcctctt 4020

tctgcacttc ttgagttgga caagttgaaa gtgaaaggat acaatgaaat gaagtatacc 4080

ccatcacggg atcgtcaatg gcatatctac acacttagaa atactgaaaa ccccaaaatg 4140

ttgcaccggg tatttttccg aacccttgtc aggcaaccca gtgtatccaa caagttttct 4200

tcgggccaga ttggtgacat ggaagttggg agtgctgaag aacctctgtc atttacatca 4260

accagcatat taagatcttt gatgactgct atagaggaat tggagcttca cgcaattaga 4320

actggccatt cacacatgta tttgcatgta ttgaaagaac aaaagcttct tgatcttgtt 4380

ccagtttcag ggaatacagt tttggatgtt ggtcaagatg aagctactgc atattcactt 4440

ttaaaagaaa tggctatgaa gatacatgaa cttgttggtg caagaatgca ccatctttct 4500

gtatgccaat gggaagtgaa acttaagttg gactgcgatg gtcctgccag tggtacctgg 4560

aggattgtaa caaccaatgt tactagtcac acttgcactg tggatatcta ccgtgagatg 4620

gaagataaag aatcacggaa gttagtatac catcccgcca ctccggcggc tggtcctctg 4680

catggtgtgg cactgaataa tccatatcag cctttgagtg tcattgatct caaacgctgt 4740

tctgctagga ataatagaac tacatactgc tatgattttc cactggcatt tgaaactgca 4800

gtgaggaagt catggtcctc tagtacctct ggtgcttcta aaggtgttga aaatgcccaa 4860

tgttatgtta aagctacaga gttggtattt gcggacaaac atgggtcatg gggcactcct 4920

ttagttcaaa tggaccggcc tgctgggctc aatgacattg gtatggtagc ttggaccttg 4980

aagatgtcca ctcctgaatt tcctagtggt agggagatta ttgttgttgc aaatgatatt 5040

acgttcagag ctggatcatt tggcccaagg gaagatgcat tttttgaagc tgttaccaac 5100

ctagcctgtg agaagaaact tcctcttatt tatttggcag caaattctgg tgctcgaatt 5160

ggcatagcag atgaagtgaa atcttgcttc cgtgttgggt ggtctgatga tggcagccct 5220

gaacgtgggt ttcagtacat ttatctaagc gaagaagact atgctcgtat tggcacttct 5280

gtcatagcac ataagatgca gctagacagt ggtgaaatta ggtgggttat tgattctgtt 5340

gtgggcaagg aagatggact tggtgtggag aatatacatg gaagtgctgc tattgccagt 5400

gcttattcta gggcatataa ggagacattt acacttacat ttgtgactgg aagaactgtt 5460

ggaataggag cttatcttgc tcgacttggc atccggtgca tacagcgtct tgaccagcct 5520

attattctta caggctattc tgcactgaac aagcttcttg ggcgggaagt gtacagctcc 5580

cacatgcagt tgggtggtcc caaaatcatg gcaactaatg gttttgtcca tcttactgtt 5640

tcagatgacc ttgaaggcgt ttctaatata ttgaggtggc tcagttatgt tcctgcctac 5700

attggtggac cacttccagt aacaacaccg ttggacccac cggacagacc tgttgcatac 5760

attcctgaga actcgtgtga tcctcgagcg gctatccgtg gtgttgatga cagccaaggg 5820

aaatggttag gtggtatgtt tgataaagac agctttgtgg aaacatttga aggttgggct 5880

aagacagtgg ttactggcag agcaaagctt ggtggaattc cagtgggtgt gatagctgtg 5940

gagactcaga ccatgatgca aactatccct gctgaccctg gtcagcttga ttcccgtgag 6000

caatctgttc ctcgtgctgg acaagtgtgg tttccagatt ctgcaaccaa gactgcgcag 6060

gcattgctgg acttcaaccg tgaaggatta cctctgttca tcctcgctaa ctggagaggc 6120

ttctctggtg gacaaagaga tctttttgaa ggaattcttc aggctggctc gactattgtt 6180

gagaacctta ggacatacaa tcagcctgcc tttgtctaca ttcccatggc tgcagagcta 6240

cgaggagggg cttgggttgt ggttgatagc aagataaacc cagaccgcat tgagtgctat 6300

gctgagagga ctgcaaaagg caatgttctg gaaccgcaag ggttaattga gatcaagttc 6360

aggtcagagg aactccagga ttgcatgagt cggcttgacc caacattaat tgatctgaaa 6420

gcaaaactcg aagtagcaaa taaaaatgga agtgctgaca caaaatcgct tcaagaaaat 6480

atagaagctc gaacaaaaca gttgatgcct ctatatactc agattgcgat acggtttgct 6540

gaattgcatg atacatccct cagaatggct gcgaaaggtg tgattaagaa agttgtggac 6600

tgggaagaat cacgatcttt cttctataag agattacgga ggaggatctc tgaggatgtt 6660

cttgcaaaag aaattagagc tgtagcaggt gagcagtttt cccaccaacc agcaatcgag 6720

ctgatcaaga aatggtattc agcttcacat gcagctgaat gggatgatga cgatgctttt 6780

gttgcttgga tggataaccc tgaaaactac aaggattata ttcaatatct taaggctcaa 6840

agagtatccc aatccctctc aagtctttca gattccagct cagatttgca agccctgcca 6900

cagggtcttt ccatgttact agataagatg gatccctcta gaagagctca acttgttgaa 6960

gaaatcagga aggtccttgg ttga 6984

<210> 8

<211> 2327

<212> PRT

<213> 水稻(Oryza sativa嘉花1号ACCase-5623突变氨基酸序列)

<400> 8

Met Thr Ser Thr His Val Ala Thr Leu Gly Val Gly Ala Gln Ala Pro

1 5 10 15

Pro Arg His Gln Lys Lys Ser Ala Gly Thr Ala Phe Val Ser Ser Gly

20 25 30

Ser Ser Arg Pro Ser Tyr Arg Lys Asn Gly Gln Arg Thr Arg Ser Leu

35 40 45

Arg Glu Glu Ser Asn Gly Gly Val Ser Asp Ser Lys Lys Leu Asn His

50 55 60

Ser Ile Arg Gln Gly Leu Ala Gly Ile Ile Asp Leu Pro Asn Asp Ala

65 70 75 80

Ala Ser Glu Val Asp Ile Ser His Gly Ser Glu Asp Pro Arg Gly Pro

85 90 95

Thr Val Pro Gly Ser Tyr Gln Met Asn Gly Ile Ile Asn Glu Thr His

100 105 110

Asn Gly Arg His Ala Ser Val Ser Lys Val Val Glu Phe Cys Thr Ala

115 120 125

Leu Gly Gly Lys Thr Pro Ile His Ser Val Leu Val Ala Asn Asn Gly

130 135 140

Met Ala Ala Ala Lys Phe Met Arg Ser Val Arg Thr Trp Ala Asn Asp

145 150 155 160

Thr Phe Gly Ser Glu Lys Ala Ile Gln Leu Ile Ala Met Ala Thr Pro

165 170 175

Glu Asp Leu Arg Ile Asn Ala Glu His Ile Arg Ile Ala Asp Gln Phe

180 185 190

Val Glu Val Pro Gly Gly Thr Asn Asn Asn Asn Tyr Ala Asn Val Gln

195 200 205

Leu Ile Val Glu Ile Ala Glu Arg Thr Gly Val Ser Ala Val Trp Pro

210 215 220

Gly Trp Gly His Ala Ser Glu Asn Pro Glu Leu Pro Asp Ala Leu Thr

225 230 235 240

Ala Lys Gly Ile Val Phe Leu Gly Pro Pro Ala Ser Ser Met His Ala

245 250 255

Leu Gly Asp Lys Val Gly Ser Ala Leu Ile Ala Gln Ala Ala Gly Val

260 265 270

Pro Thr Leu Ala Trp Ser Gly Ser His Val Glu Val Pro Leu Glu Cys

275 280 285

Cys Leu Asp Ser Ile Pro Asp Glu Met Tyr Arg Lys Ala Cys Val Thr

290 295 300

Thr Thr Glu Glu Ala Val Ala Ser Cys Gln Val Val Gly Tyr Pro Ala

305 310 315 320

Met Ile Lys Ala Ser Trp Gly Gly Gly Gly Lys Gly Ile Arg Lys Val

325 330 335

His Asn Asp Asp Glu Val Arg Thr Leu Phe Lys Gln Val Gln Gly Glu

340 345 350

Val Pro Gly Ser Pro Ile Phe Ile Met Arg Leu Ala Ala Gln Ser Arg

355 360 365

His Leu Glu Val Gln Leu Leu Cys Asp Gln Tyr Gly Asn Val Ala Ala

370 375 380

Leu His Ser Arg Asp Cys Ser Val Gln Arg Arg His Gln Lys Ile Ile

385 390 395 400

Glu Glu Gly Pro Val Thr Val Ala Pro Arg Glu Thr Val Lys Glu Leu

405 410 415

Glu Gln Ala Ala Arg Arg Leu Ala Lys Ala Val Gly Tyr Val Gly Ala

420 425 430

Ala Thr Val Glu Tyr Leu Tyr Ser Met Glu Thr Gly Glu Tyr Tyr Phe

435 440 445

Leu Glu Leu Asn Pro Arg Leu Gln Val Glu His Pro Val Thr Glu Trp

450 455 460

Ile Ala Glu Val Asn Leu Pro Ala Ala Gln Val Ala Val Gly Met Gly

465 470 475 480

Ile Pro Leu Trp Gln Ile Pro Glu Ile Arg Arg Phe Tyr Gly Met Asn

485 490 495

His Gly Gly Gly Tyr Asp Leu Trp Arg Lys Thr Ala Ala Leu Ala Thr

500 505 510

Pro Phe Asn Phe Asp Glu Val Asp Ser Lys Trp Pro Lys Gly His Cys

515 520 525

Val Ala Val Arg Ile Thr Ser Glu Asp Pro Asp Asp Gly Phe Lys Pro

530 535 540

Thr Gly Gly Lys Val Lys Glu Ile Ser Phe Lys Ser Lys Pro Asn Val

545 550 555 560

Trp Ala Tyr Phe Ser Val Lys Ser Gly Gly Gly Ile His Glu Phe Ala

565 570 575

Asp Ser Gln Phe Gly His Val Phe Ala Tyr Gly Thr Thr Arg Ser Ala

580 585 590

Ala Ile Thr Thr Met Ala Leu Ala Leu Lys Glu Val Gln Ile Arg Gly

595 600 605

Glu Ile His Ser Asn Val Asp Tyr Thr Val Asp Leu Leu Asn Ala Ser

610 615 620

Asp Phe Arg Glu Asn Lys Ile His Thr Gly Trp Leu Asp Thr Arg Ile

625 630 635 640

Ala Met Arg Val Gln Ala Glu Arg Pro Pro Trp Tyr Ile Ser Val Val

645 650 655

Gly Gly Ala Leu Tyr Lys Thr Val Thr Ala Asn Thr Ala Thr Val Ser

660 665 670

Asp Tyr Val Gly Tyr Leu Thr Lys Gly Gln Ile Pro Pro Lys His Ile

675 680 685

Ser Leu Val Tyr Thr Thr Val Ala Leu Asn Ile Asp Gly Lys Lys Tyr

690 695 700

Thr Ile Asp Thr Val Arg Ser Gly His Gly Ser Tyr Arg Leu Arg Met

705 710 715 720

Asn Gly Ser Thr Val Asp Ala Asn Val Gln Ile Leu Cys Asp Gly Gly

725 730 735

Leu Leu Met Gln Leu Asp Gly Asn Ser His Val Ile Tyr Ala Glu Glu

740 745 750

Glu Ala Ser Gly Thr Arg Leu Leu Ile Asp Gly Lys Thr Cys Met Leu

755 760 765

Gln Asn Asp His Asp Pro Ser Lys Leu Leu Ala Glu Thr Pro Cys Lys

770 775 780

Leu Leu Arg Phe Leu Val Ala Asp Gly Ala His Val Asp Ala Asp Val

785 790 795 800

Pro Tyr Ala Glu Val Glu Val Met Lys Met Cys Met Pro Leu Leu Ser

805 810 815

Pro Ala Ser Gly Val Ile His Val Val Met Ser Glu Gly Gln Ala Met

820 825 830

Gln Ala Gly Asp Leu Ile Ala Arg Leu Asp Leu Asp Asp Pro Ser Ala

835 840 845

Val Lys Arg Ala Glu Pro Phe Glu Asp Thr Phe Pro Gln Met Gly Leu

850 855 860

Pro Ile Ala Ala Ser Gly Gln Val His Lys Leu Cys Ala Ala Ser Leu

865 870 875 880

Asn Ala Cys Arg Met Ile Leu Ala Gly Tyr Glu His Asp Ile Asp Lys

885 890 895

Val Val Pro Glu Leu Val Tyr Cys Leu Asp Thr Pro Glu Leu Pro Phe

900 905 910

Leu Gln Trp Glu Glu Leu Met Ser Val Leu Ala Thr Arg Leu Pro Arg

915 920 925

Asn Leu Lys Ser Glu Leu Glu Gly Lys Tyr Glu Glu Tyr Lys Val Lys

930 935 940

Phe Asp Ser Gly Ile Ile Asn Asp Phe Pro Ala Asn Met Leu Arg Val

945 950 955 960

Ile Ile Glu Glu Asn Leu Ala Cys Gly Ser Glu Lys Glu Lys Ala Thr

965 970 975

Asn Glu Arg Leu Val Glu Pro Leu Met Ser Leu Leu Lys Ser Tyr Glu

980 985 990

Gly Gly Arg Glu Ser His Ala His Phe Val Val Lys Ser Leu Phe Glu

995 1000 1005

Glu Tyr Leu Tyr Val Glu Glu Leu Phe Ser Asp Gly Ile Gln Ser Asp

1010 1015 1020

Val Ile Glu Arg Leu Arg Leu Gln His Ser Lys Asp Leu Gln Lys Val

1025 1030 1035 1040

Val Asp Ile Val Leu Ser His Gln Ser Val Arg Asn Lys Thr Lys Leu

1045 1050 1055

Ile Leu Lys Leu Met Glu Ser Leu Val Tyr Pro Asn Pro Ala Ala Tyr

1060 1065 1070

Arg Asp Gln Leu Ile Arg Phe Ser Ser Leu Asn His Lys Ala Tyr Tyr

1075 1080 1085

Lys Leu Ala Leu Lys Ala Ser Glu Leu Leu Glu Gln Thr Lys Leu Ser

1090 1095 1100

Glu Leu Arg Ala Arg Ile Ala Arg Ser Leu Ser Glu Leu Glu Met Phe

1105 1110 1115 1120

Thr Glu Glu Ser Lys Gly Leu Ser Met His Lys Arg Glu Ile Ala Ile

1125 1130 1135

Lys Glu Ser Met Glu Asp Leu Val Thr Ala Pro Leu Pro Val Glu Asp

1140 1145 1150

Ala Leu Ile Ser Leu Phe Asp Cys Ser Asp Thr Thr Val Gln Gln Arg

1155 1160 1165

Val Ile Glu Thr Tyr Ile Ala Arg Leu Tyr Gln Pro His Leu Val Lys

1170 1175 1180

Asp Ser Ile Lys Met Lys Trp Ile Glu Ser Gly Val Ile Ala Leu Trp

1185 1190 1195 1200

Glu Phe Pro Glu Gly His Phe Asp Ala Arg Asn Gly Gly Ala Val Leu

1205 1210 1215

Gly Asp Lys Arg Trp Gly Ala Met Val Ile Val Lys Ser Leu Glu Ser

1220 1225 1230

Leu Ser Met Ala Ile Arg Phe Ala Leu Lys Glu Thr Ser His Tyr Thr

1235 1240 1245

Ser Ser Glu Gly Asn Met Met His Ile Ala Leu Leu Gly Ala Asp Asn

1250 1255 1260

Lys Met His Ile Ile Gln Glu Ser Gly Asp Asp Ala Asp Arg Ile Ala

1265 1270 1275 1280

Lys Leu Pro Leu Ile Leu Lys Asp Asn Val Thr Asp Leu His Ala Ser

1285 1290 1295

Gly Val Lys Thr Ile Ser Phe Ile Val Gln Arg Asp Glu Ala Arg Met

1300 1305 1310

Thr Met Arg Arg Thr Phe Leu Trp Ser Asp Glu Lys Leu Ser Tyr Glu

1315 1320 1325

Glu Glu Pro Ile Leu Arg His Val Glu Pro Pro Leu Ser Ala Leu Leu

1330 1335 1340

Glu Leu Asp Lys Leu Lys Val Lys Gly Tyr Asn Glu Met Lys Tyr Thr

1345 1350 1355 1360

Pro Ser Arg Asp Arg Gln Trp His Ile Tyr Thr Leu Arg Asn Thr Glu

1365 1370 1375

Asn Pro Lys Met Leu His Arg Val Phe Phe Arg Thr Leu Val Arg Gln

1380 1385 1390

Pro Ser Val Ser Asn Lys Phe Ser Ser Gly Gln Ile Gly Asp Met Glu

1395 1400 1405

Val Gly Ser Ala Glu Glu Pro Leu Ser Phe Thr Ser Thr Ser Ile Leu

1410 1415 1420

Arg Ser Leu Met Thr Ala Ile Glu Glu Leu Glu Leu His Ala Ile Arg

1425 1430 1435 1440

Thr Gly His Ser His Met Tyr Leu His Val Leu Lys Glu Gln Lys Leu

1445 1450 1455

Leu Asp Leu Val Pro Val Ser Gly Asn Thr Val Leu Asp Val Gly Gln

1460 1465 1470

Asp Glu Ala Thr Ala Tyr Ser Leu Leu Lys Glu Met Ala Met Lys Ile

1475 1480 1485

His Glu Leu Val Gly Ala Arg Met His His Leu Ser Val Cys Gln Trp

1490 1495 1500

Glu Val Lys Leu Lys Leu Asp Cys Asp Gly Pro Ala Ser Gly Thr Trp

1505 1510 1515 1520

Arg Ile Val Thr Thr Asn Val Thr Ser His Thr Cys Thr Val Asp Ile

1525 1530 1535

Tyr Arg Glu Met Glu Asp Lys Glu Ser Arg Lys Leu Val Tyr His Pro

1540 1545 1550

Ala Thr Pro Ala Ala Gly Pro Leu His Gly Val Ala Leu Asn Asn Pro

1555 1560 1565

Tyr Gln Pro Leu Ser Val Ile Asp Leu Lys Arg Cys Ser Ala Arg Asn

1570 1575 1580

Asn Arg Thr Thr Tyr Cys Tyr Asp Phe Pro Leu Ala Phe Glu Thr Ala

1585 1590 1595 1600

Val Arg Lys Ser Trp Ser Ser Ser Thr Ser Gly Ala Ser Lys Gly Val

1605 1610 1615

Glu Asn Ala Gln Cys Tyr Val Lys Ala Thr Glu Leu Val Phe Ala Asp

1620 1625 1630

Lys His Gly Ser Trp Gly Thr Pro Leu Val Gln Met Asp Arg Pro Ala

1635 1640 1645

Gly Leu Asn Asp Ile Gly Met Val Ala Trp Thr Leu Lys Met Ser Thr

1650 1655 1660

Pro Glu Phe Pro Ser Gly Arg Glu Ile Ile Val Val Ala Asn Asp Ile

1665 1670 1675 1680

Thr Phe Arg Ala Gly Ser Phe Gly Pro Arg Glu Asp Ala Phe Phe Glu

1685 1690 1695

Ala Val Thr Asn Leu Ala Cys Glu Lys Lys Leu Pro Leu Ile Tyr Leu

1700 1705 1710

Ala Ala Asn Ser Gly Ala Arg Ile Gly Ile Ala Asp Glu Val Lys Ser

1715 1720 1725

Cys Phe Arg Val Gly Trp Ser Asp Asp Gly Ser Pro Glu Arg Gly Phe

1730 1735 1740

Gln Tyr Ile Tyr Leu Ser Glu Glu Asp Tyr Ala Arg Ile Gly Thr Ser

1745 1750 1755 1760

Val Ile Ala His Lys Met Gln Leu Asp Ser Gly Glu Ile Arg Trp Val

1765 1770 1775

Ile Asp Ser Val Val Gly Lys Glu Asp Gly Leu Gly Val Glu Asn Ile

1780 1785 1790

His Gly Ser Ala Ala Ile Ala Ser Ala Tyr Ser Arg Ala Tyr Lys Glu

1795 1800 1805

Thr Phe Thr Leu Thr Phe Val Thr Gly Arg Thr Val Gly Ile Gly Ala

1810 1815 1820

Tyr Leu Ala Arg Leu Gly Ile Arg Cys Ile Gln Arg Leu Asp Gln Pro

1825 1830 1835 1840

Ile Ile Leu Thr Gly Tyr Ser Ala Leu Asn Lys Leu Leu Gly Arg Glu

1845 1850 1855

Val Tyr Ser Ser His Met Gln Leu Gly Gly Pro Lys Ile Met Ala Thr

1860 1865 1870

Asn Gly Phe Val His Leu Thr Val Ser Asp Asp Leu Glu Gly Val Ser

1875 1880 1885

Asn Ile Leu Arg Trp Leu Ser Tyr Val Pro Ala Tyr Ile Gly Gly Pro

1890 1895 1900

Leu Pro Val Thr Thr Pro Leu Asp Pro Pro Asp Arg Pro Val Ala Tyr

1905 1910 1915 1920

Ile Pro Glu Asn Ser Cys Asp Pro Arg Ala Ala Ile Arg Gly Val Asp

1925 1930 1935

Asp Ser Gln Gly Lys Trp Leu Gly Gly Met Phe Asp Lys Asp Ser Phe

1940 1945 1950

Val Glu Thr Phe Glu Gly Trp Ala Lys Thr Val Val Thr Gly Arg Ala

1955 1960 1965

Lys Leu Gly Gly Ile Pro Val Gly Val Ile Ala Val Glu Thr Gln Thr

1970 1975 1980

Met Met Gln Thr Ile Pro Ala Asp Pro Gly Gln Leu Asp Ser Arg Glu

1985 1990 1995 2000

Gln Ser Val Pro Arg Ala Gly Gln Val Trp Phe Pro Asp Ser Ala Thr

2005 2010 2015

Lys Thr Ala Gln Ala Leu Leu Asp Phe Asn Arg Glu Gly Leu Pro Leu

2020 2025 2030

Phe Ile Leu Ala Asn Trp Arg Gly Phe Ser Gly Gly Gln Arg Asp Leu

2035 2040 2045

Phe Glu Gly Ile Leu Gln Ala Gly Ser Thr Ile Val Glu Asn Leu Arg

2050 2055 2060

Thr Tyr Asn Gln Pro Ala Phe Val Tyr Ile Pro Met Ala Ala Glu Leu

2065 2070 2075 2080

Arg Gly Gly Ala Trp Val Val Val Asp Ser Lys Ile Asn Pro Asp Arg

2085 2090 2095

Ile Glu Cys Tyr Ala Glu Arg Thr Ala Lys Gly Asn Val Leu Glu Pro

2100 2105 2110

Gln Gly Leu Ile Glu Ile Lys Phe Arg Ser Glu Glu Leu Gln Asp Cys

2115 2120 2125

Met Ser Arg Leu Asp Pro Thr Leu Ile Asp Leu Lys Ala Lys Leu Glu

2130 2135 2140

Val Ala Asn Lys Asn Gly Ser Ala Asp Thr Lys Ser Leu Gln Glu Asn

2145 2150 2155 2160

Ile Glu Ala Arg Thr Lys Gln Leu Met Pro Leu Tyr Thr Gln Ile Ala

2165 2170 2175

Ile Arg Phe Ala Glu Leu His Asp Thr Ser Leu Arg Met Ala Ala Lys

2180 2185 2190

Gly Val Ile Lys Lys Val Val Asp Trp Glu Glu Ser Arg Ser Phe Phe

2195 2200 2205

Tyr Lys Arg Leu Arg Arg Arg Ile Ser Glu Asp Val Leu Ala Lys Glu

2210 2215 2220

Ile Arg Ala Val Ala Gly Glu Gln Phe Ser His Gln Pro Ala Ile Glu

2225 2230 2235 2240

Leu Ile Lys Lys Trp Tyr Ser Ala Ser His Ala Ala Glu Trp Asp Asp

2245 2250 2255

Asp Asp Ala Phe Val Ala Trp Met Asp Asn Pro Glu Asn Tyr Lys Asp

2260 2265 2270

Tyr Ile Gln Tyr Leu Lys Ala Gln Arg Val Ser Gln Ser Leu Ser Ser

2275 2280 2285

Leu Ser Asp Ser Ser Ser Asp Leu Gln Ala Leu Pro Gln Gly Leu Ser

2290 2295 2300

Met Leu Leu Asp Lys Met Asp Pro Ser Arg Arg Ala Gln Leu Val Glu

2305 2310 2315 2320

Glu Ile Arg Lys Val Leu Gly

2325

<210> 9

<211> 6984

<212> DNA

<213> 水稻(Oryza sativa嘉花1号ACCase-6028突变核苷酸序列)

<400> 9

atgacatcca cacatgtggc gacattggga gttggtgccc aggcacctcc tcgtcaccag 60

aaaaagtcag ctggcactgc atttgtatca tctgggtcat caagaccctc ataccgaaag 120

aatggtcagc gtactcggtc acttagggaa gaaagcaatg gaggagtgtc tgattccaaa 180

aagcttaacc actctattcg ccaaggtctt gctggcatca ttgacctccc aaatgacgca 240

gcttcagaag ttgatatttc acatggttcc gaagatccca gggggcctac ggtcccaggt 300

tcctaccaaa tgaatgggat tatcaatgaa acacataatg ggaggcatgc ttcagtctcc 360

aaggttgttg agttttgtac ggcacttggt ggcaaaacac caattcacag tgtattagtg 420

gccaacaatg gaatggcagc agctaagttc atgcggagtg tccgaacatg ggctaatgat 480

acttttggat cagagaaggc aattcagctg atagctatgg caactccgga ggatctgagg 540

ataaatgcag agcacatcag aattgccgat caatttgtag aggtacctgg tggaacaaac 600

aacaacaact atgcaaatgt ccaactcata gtggagatag cagagagaac aggtgtttct 660

gctgtttggc ctggttgggg tcatgcatct gagaatcctg aacttccaga tgcgctgact 720

gcaaaaggaa ttgtttttct tgggccacca gcatcatcaa tgcatgcatt aggagacaag 780

gttggctcag ctctcattgc tcaagcagct ggagttccaa cacttgcttg gagtggatca 840

catgtggaag ttcctctgga gtgttgcttg gactcaatac ctgatgagat gtatagaaaa 900

gcttgtgtta ctaccacaga ggaagcagtt gcaagttgtc aggtggttgg ttatcctgcc 960

atgattaagg catcttgggg tggtggtggt aaaggaataa ggaaggttca taatgatgat 1020

gaggttagga cattatttaa gcaagttcaa ggcgaagtac ctggttcccc aatatttatc 1080

atgaggctag ctgctcagag tcgacatctt gaagttcagt tgctttgtga tcaatatggc 1140

aacgtagcag cacttcacag tcgagattgc agtgtacaac ggcgacacca aaagataatc 1200

gaggaaggac cagttactgt tgctcctcgt gagactgtga aagagcttga gcaggcagca 1260

cggaggcttg ctaaagctgt gggttatgtt ggtgctgcta ctgttgaata cctttacagc 1320

atggaaactg gtgaatatta ttttctggaa cttaatccac ggctacaggt tgagcatcct 1380

gtcactgagt ggatagctga agtaaatttg cctgcggctc aagttgctgt tggaatgggt 1440

ataccccttt ggcagattcc agagatcagg cgcttctacg gaatgaacca tggaggaggc 1500

tatgaccttt ggaggaaaac agcagctcta gcgactccat ttaactttga tgaagtagat 1560

tctaaatggc caaaaggcca ctgcgtagct gttagaataa ctagcgagga tccagatgat 1620

gggtttaagc ctactggtgg aaaagtaaag gagataagtt tcaagagtaa accaaatgtt 1680

tgggcctatt tctcagtaaa gtctggtgga ggcatccatg aattcgctga ttctcagttc 1740

ggacatgttt ttgcgtatgg aactactaga tcggcagcaa taactaccat ggctcttgca 1800

ctaaaagagg ttcaaattcg tggagaaatt cattcaaacg tagactacac agttgaccta 1860

ttaaatgcct cagattttag agaaaataag attcatactg gttggctgga taccaggata 1920

gccatgcgtg ttcaagctga gaggcctcca tggtatattt cagtcgttgg aggggcttta 1980

tataaaacag taactgccaa cacggccact gtttctgatt atgttggtta tcttaccaag 2040

ggccagattc caccaaagca tatatccctt gtctatacga ctgttgcttt gaatatagat 2100

gggaaaaaat atacaatcga tactgtgagg agtggacatg gtagctacag attgcgaatg 2160

aatggatcaa cggttgacgc aaatgtacaa atattatgtg atggtgggct tttaatgcag 2220

ctggatggaa acagccatgt aatttatgct gaagaagagg ccagtggtac acgacttctt 2280

attgatggaa agacatgcat gttacagaat gaccatgacc catcaaagtt attagctgag 2340

acaccatgca aacttcttcg tttcttggtt gctgatggtg ctcatgttga tgctgatgta 2400

ccatatgcgg aagttgaggt tatgaagatg tgcatgcccc tcttatcacc cgcttctggt 2460

gtcatacatg ttgtaatgtc tgagggccaa gcaatgcagg ctggtgatct tatagctagg 2520

ctggatcttg atgacccttc tgctgttaag agagctgagc cgttcgaaga tacttttcca 2580

caaatgggtc tccctattgc tgcttctggc caagttcaca aattatgtgc tgcaagtctg 2640

aatgcttgtc gaatgatcct tgcggggtat gagcatgata ttgacaaggt tgtgccagag 2700

ttggtatact gcctagacac tccggagctt cctttcctgc agtgggagga gcttatgtct 2760

gttttagcaa ctagacttcc aagaaatctt aaaagtgagt tggagggcaa atatgaggaa 2820

tacaaagtaa aatttgactc tgggataatc aatgatttcc ctgccaatat gctacgagtg 2880

ataattgagg aaaatcttgc atgtggttct gagaaggaga aggctacaaa tgagaggctt 2940

gttgagcctc ttatgagcct actgaagtca tatgagggtg ggagagaaag tcatgctcac 3000

tttgttgtca agtccctttt tgaggagtat ctctatgttg aagaattgtt cagtgatgga 3060

attcagtctg atgtgattga gcgtctgcgc cttcaacata gtaaagacct acagaaggtc 3120

gtagacattg tgttgtccca ccagagtgtt agaaataaaa ctaagctgat actaaaactc 3180

atggagagtc tggtctatcc aaatcctgct gcctacaggg atcaattgat tcgcttttct 3240

tcccttaatc acaaagcgta ttacaagttg gcacttaaag ctagtgaact tcttgaacaa 3300

acaaaactta gtgagctccg tgcaagaata gcaaggagcc tttcagagct ggagatgttt 3360

actgaggaaa gcaagggtct ctccatgcat aagcgagaaa ttgccattaa ggagagcatg 3420

gaagatttag tcactgctcc actgccagtt gaagatgcgc tcatttcttt atttgattgt 3480

agtgatacaa ctgttcaaca gagagtgatt gagacttata tagctcgatt ataccagcct 3540

catcttgtaa aggacagtat caaaatgaaa tggatagaat cgggtgttat tgctttatgg 3600

gaatttcctg aagggcattt tgatgcaaga aatggaggag cggttcttgg tgacaaaaga 3660

tggggtgcca tggtcattgt caagtctctt gaatcacttt caatggccat tagatttgca 3720

ctaaaggaga catcacacta cactagctct gagggcaata tgatgcatat tgctttgttg 3780

ggtgctgata ataagatgca tataattcaa gaaagtggtg atgatgctga cagaatagcc 3840

aaacttccct tgatactaaa ggataatgta accgatctgc atgcctctgg tgtgaaaaca 3900

ataagtttca ttgttcaaag agatgaagca cggatgacaa tgcgtcgtac cttcctttgg 3960

tctgatgaaa agctttctta tgaggaagag ccaattctcc ggcatgtgga acctcctctt 4020

tctgcacttc ttgagttgga caagttgaaa gtgaaaggat acaatgaaat gaagtatacc 4080

ccatcacggg atcgtcaatg gcatatctac acacttagaa atactgaaaa ccccaaaatg 4140

ttgcaccggg tatttttccg aacccttgtc aggcaaccca gtgtatccaa caagttttct 4200

tcgggccaga ttggtgacat ggaagttggg agtgctgaag aacctctgtc atttacatca 4260

accagcatat taagatcttt gatgactgct atagaggaat tggagcttca cgcaattaga 4320

actggccatt cacacatgta tttgcatgta ttgaaagaac aaaagcttct tgatcttgtt 4380

ccagtttcag ggaatacagt tttggatgtt ggtcaagatg aagctactgc atattcactt 4440

ttaaaagaaa tggctatgaa gatacatgaa cttgttggtg caagaatgca ccatctttct 4500

gtatgccaat gggaagtgaa acttaagttg gactgcgatg gtcctgccag tggtacctgg 4560

aggattgtaa caaccaatgt tactagtcac acttgcactg tggatatcta ccgtgagatg 4620

gaagataaag aatcacggaa gttagtatac catcccgcca ctccggcggc tggtcctctg 4680

catggtgtgg cactgaataa tccatatcag cctttgagtg tcattgatct caaacgctgt 4740

tctgctagga ataatagaac tacatactgc tatgattttc cactggcatt tgaaactgca 4800

gtgaggaagt catggtcctc tagtacctct ggtgcttcta aaggtgttga aaatgcccaa 4860

tgttatgtta aagctacaga gttggtattt gcggacaaac atgggtcatg gggcactcct 4920

ttagttcaaa tggaccggcc tgctgggctc aatgacattg gtatggtagc ttggaccttg 4980

aagatgtcca ctcctgaatt tcctagtggt agggagatta ttgttgttgc aaatgatatt 5040

acgttcagag ctggatcatt tggcccaagg gaagatgcat tttttgaagc tgttaccaac 5100

ctagcctgtg agaagaaact tcctcttatt tatttggcag caaattctgg tgctcgaatt 5160

ggcatagcag atgaagtgaa atcttgcttc cgtgttgggt ggtctgatga tggcagccct 5220

gaacgtgggt ttcagtacat ttatctaagc gaagaagact atgctcgtat tggcacttct 5280

gtcatagcac ataagatgca gctagacagt ggtgaaatta ggtgggttat tgattctgtt 5340

gtgggcaagg aagatggact tggtgtggag aatatacatg gaagtgctgc tattgccagt 5400

gcttattcta gggcatataa ggagacattt acacttacat ttgtgactgg aagaactgtt 5460

ggaataggag cttatcttgc tcgacttggc atccggtgca tacagcgtct tgaccagcct 5520

attattctta caggctattc tgcactgaac aagcttcttg ggcgggaagt gtacagctcc 5580

cacatgcagt tgggtggtcc caaaatcatg gcaactaatg gtgttgtcca tcttactgtt 5640

tcagatgacc ttgaaggcgt ttctaatata ttgaggtggc tcagttatgt tcctgcctac 5700

attggtggac cacttccagt aacaacaccg ttggacccac cggacagacc tgttgcatac 5760

attcctgaga actcgtgtga tcctcgagcg gctatccgtg gtgttgatga cagccaaggg 5820

aaatggttag gtggtatgtt tgataaagac agctttgtgg aaacatttga aggttgggct 5880

aagacagtgg ttactggcag agcaaagctt ggtggaattc cagtgggtgt gatagctgtg 5940

gagactcaga ccatgatgca aactatccct gctgaccctg gtcagcttga ttcccgtgag 6000

caatctgttc ctcgtgctgg acaagtgggg tttccagatt ctgcaaccaa gactgcgcag 6060

gcattgctgg acttcaaccg tgaaggatta cctctgttca tcctcgctaa ctggagaggc 6120

ttctctggtg gacaaagaga tctttttgaa ggaattcttc aggctggctc gactattgtt 6180

gagaacctta ggacatacaa tcagcctgcc tttgtctaca ttcccatggc tgcagagcta 6240

cgaggagggg cttgggttgt ggttgatagc aagataaacc cagaccgcat tgagtgctat 6300

gctgagagga ctgcaaaagg caatgttctg gaaccgcaag ggttaattga gatcaagttc 6360

aggtcagagg aactccagga ttgcatgagt cggcttgacc caacattaat tgatctgaaa 6420

gcaaaactcg aagtagcaaa taaaaatgga agtgctgaca caaaatcgct tcaagaaaat 6480

atagaagctc gaacaaaaca gttgatgcct ctatatactc agattgcgat acggtttgct 6540

gaattgcatg atacatccct cagaatggct gcgaaaggtg tgattaagaa agttgtggac 6600

tgggaagaat cacgatcttt cttctataag agattacgga ggaggatctc tgaggatgtt 6660

cttgcaaaag aaattagagc tgtagcaggt gagcagtttt cccaccaacc agcaatcgag 6720

ctgatcaaga aatggtattc agcttcacat gcagctgaat gggatgatga cgatgctttt 6780

gttgcttgga tggataaccc tgaaaactac aaggattata ttcaatatct taaggctcaa 6840

agagtatccc aatccctctc aagtctttca gattccagct cagatttgca agccctgcca 6900

cagggtcttt ccatgttact agataagatg gatccctcta gaagagctca acttgttgaa 6960

gaaatcagga aggtccttgg ttga 6984

<210> 10

<211> 2327

<212> PRT

<213> 水稻(Oryza sativa嘉花1号ACCase-6028突变氨基酸序列)

<400> 10

Met Thr Ser Thr His Val Ala Thr Leu Gly Val Gly Ala Gln Ala Pro

1 5 10 15

Pro Arg His Gln Lys Lys Ser Ala Gly Thr Ala Phe Val Ser Ser Gly

20 25 30

Ser Ser Arg Pro Ser Tyr Arg Lys Asn Gly Gln Arg Thr Arg Ser Leu

35 40 45

Arg Glu Glu Ser Asn Gly Gly Val Ser Asp Ser Lys Lys Leu Asn His

50 55 60

Ser Ile Arg Gln Gly Leu Ala Gly Ile Ile Asp Leu Pro Asn Asp Ala

65 70 75 80

Ala Ser Glu Val Asp Ile Ser His Gly Ser Glu Asp Pro Arg Gly Pro

85 90 95

Thr Val Pro Gly Ser Tyr Gln Met Asn Gly Ile Ile Asn Glu Thr His

100 105 110

Asn Gly Arg His Ala Ser Val Ser Lys Val Val Glu Phe Cys Thr Ala

115 120 125

Leu Gly Gly Lys Thr Pro Ile His Ser Val Leu Val Ala Asn Asn Gly

130 135 140

Met Ala Ala Ala Lys Phe Met Arg Ser Val Arg Thr Trp Ala Asn Asp

145 150 155 160

Thr Phe Gly Ser Glu Lys Ala Ile Gln Leu Ile Ala Met Ala Thr Pro

165 170 175

Glu Asp Leu Arg Ile Asn Ala Glu His Ile Arg Ile Ala Asp Gln Phe

180 185 190

Val Glu Val Pro Gly Gly Thr Asn Asn Asn Asn Tyr Ala Asn Val Gln

195 200 205

Leu Ile Val Glu Ile Ala Glu Arg Thr Gly Val Ser Ala Val Trp Pro

210 215 220

Gly Trp Gly His Ala Ser Glu Asn Pro Glu Leu Pro Asp Ala Leu Thr

225 230 235 240

Ala Lys Gly Ile Val Phe Leu Gly Pro Pro Ala Ser Ser Met His Ala

245 250 255

Leu Gly Asp Lys Val Gly Ser Ala Leu Ile Ala Gln Ala Ala Gly Val

260 265 270

Pro Thr Leu Ala Trp Ser Gly Ser His Val Glu Val Pro Leu Glu Cys

275 280 285

Cys Leu Asp Ser Ile Pro Asp Glu Met Tyr Arg Lys Ala Cys Val Thr

290 295 300

Thr Thr Glu Glu Ala Val Ala Ser Cys Gln Val Val Gly Tyr Pro Ala

305 310 315 320

Met Ile Lys Ala Ser Trp Gly Gly Gly Gly Lys Gly Ile Arg Lys Val

325 330 335

His Asn Asp Asp Glu Val Arg Thr Leu Phe Lys Gln Val Gln Gly Glu

340 345 350

Val Pro Gly Ser Pro Ile Phe Ile Met Arg Leu Ala Ala Gln Ser Arg

355 360 365

His Leu Glu Val Gln Leu Leu Cys Asp Gln Tyr Gly Asn Val Ala Ala

370 375 380

Leu His Ser Arg Asp Cys Ser Val Gln Arg Arg His Gln Lys Ile Ile

385 390 395 400

Glu Glu Gly Pro Val Thr Val Ala Pro Arg Glu Thr Val Lys Glu Leu

405 410 415

Glu Gln Ala Ala Arg Arg Leu Ala Lys Ala Val Gly Tyr Val Gly Ala

420 425 430

Ala Thr Val Glu Tyr Leu Tyr Ser Met Glu Thr Gly Glu Tyr Tyr Phe

435 440 445

Leu Glu Leu Asn Pro Arg Leu Gln Val Glu His Pro Val Thr Glu Trp

450 455 460

Ile Ala Glu Val Asn Leu Pro Ala Ala Gln Val Ala Val Gly Met Gly

465 470 475 480

Ile Pro Leu Trp Gln Ile Pro Glu Ile Arg Arg Phe Tyr Gly Met Asn

485 490 495

His Gly Gly Gly Tyr Asp Leu Trp Arg Lys Thr Ala Ala Leu Ala Thr

500 505 510

Pro Phe Asn Phe Asp Glu Val Asp Ser Lys Trp Pro Lys Gly His Cys

515 520 525

Val Ala Val Arg Ile Thr Ser Glu Asp Pro Asp Asp Gly Phe Lys Pro

530 535 540

Thr Gly Gly Lys Val Lys Glu Ile Ser Phe Lys Ser Lys Pro Asn Val

545 550 555 560

Trp Ala Tyr Phe Ser Val Lys Ser Gly Gly Gly Ile His Glu Phe Ala

565 570 575

Asp Ser Gln Phe Gly His Val Phe Ala Tyr Gly Thr Thr Arg Ser Ala

580 585 590

Ala Ile Thr Thr Met Ala Leu Ala Leu Lys Glu Val Gln Ile Arg Gly

595 600 605

Glu Ile His Ser Asn Val Asp Tyr Thr Val Asp Leu Leu Asn Ala Ser

610 615 620

Asp Phe Arg Glu Asn Lys Ile His Thr Gly Trp Leu Asp Thr Arg Ile

625 630 635 640

Ala Met Arg Val Gln Ala Glu Arg Pro Pro Trp Tyr Ile Ser Val Val

645 650 655

Gly Gly Ala Leu Tyr Lys Thr Val Thr Ala Asn Thr Ala Thr Val Ser

660 665 670

Asp Tyr Val Gly Tyr Leu Thr Lys Gly Gln Ile Pro Pro Lys His Ile

675 680 685

Ser Leu Val Tyr Thr Thr Val Ala Leu Asn Ile Asp Gly Lys Lys Tyr

690 695 700

Thr Ile Asp Thr Val Arg Ser Gly His Gly Ser Tyr Arg Leu Arg Met

705 710 715 720

Asn Gly Ser Thr Val Asp Ala Asn Val Gln Ile Leu Cys Asp Gly Gly

725 730 735

Leu Leu Met Gln Leu Asp Gly Asn Ser His Val Ile Tyr Ala Glu Glu

740 745 750

Glu Ala Ser Gly Thr Arg Leu Leu Ile Asp Gly Lys Thr Cys Met Leu

755 760 765

Gln Asn Asp His Asp Pro Ser Lys Leu Leu Ala Glu Thr Pro Cys Lys

770 775 780

Leu Leu Arg Phe Leu Val Ala Asp Gly Ala His Val Asp Ala Asp Val

785 790 795 800

Pro Tyr Ala Glu Val Glu Val Met Lys Met Cys Met Pro Leu Leu Ser

805 810 815

Pro Ala Ser Gly Val Ile His Val Val Met Ser Glu Gly Gln Ala Met

820 825 830

Gln Ala Gly Asp Leu Ile Ala Arg Leu Asp Leu Asp Asp Pro Ser Ala

835 840 845

Val Lys Arg Ala Glu Pro Phe Glu Asp Thr Phe Pro Gln Met Gly Leu

850 855 860

Pro Ile Ala Ala Ser Gly Gln Val His Lys Leu Cys Ala Ala Ser Leu

865 870 875 880

Asn Ala Cys Arg Met Ile Leu Ala Gly Tyr Glu His Asp Ile Asp Lys

885 890 895

Val Val Pro Glu Leu Val Tyr Cys Leu Asp Thr Pro Glu Leu Pro Phe

900 905 910

Leu Gln Trp Glu Glu Leu Met Ser Val Leu Ala Thr Arg Leu Pro Arg

915 920 925

Asn Leu Lys Ser Glu Leu Glu Gly Lys Tyr Glu Glu Tyr Lys Val Lys

930 935 940

Phe Asp Ser Gly Ile Ile Asn Asp Phe Pro Ala Asn Met Leu Arg Val

945 950 955 960

Ile Ile Glu Glu Asn Leu Ala Cys Gly Ser Glu Lys Glu Lys Ala Thr

965 970 975

Asn Glu Arg Leu Val Glu Pro Leu Met Ser Leu Leu Lys Ser Tyr Glu

980 985 990

Gly Gly Arg Glu Ser His Ala His Phe Val Val Lys Ser Leu Phe Glu

995 1000 1005

Glu Tyr Leu Tyr Val Glu Glu Leu Phe Ser Asp Gly Ile Gln Ser Asp

1010 1015 1020

Val Ile Glu Arg Leu Arg Leu Gln His Ser Lys Asp Leu Gln Lys Val

1025 1030 1035 1040

Val Asp Ile Val Leu Ser His Gln Ser Val Arg Asn Lys Thr Lys Leu

1045 1050 1055

Ile Leu Lys Leu Met Glu Ser Leu Val Tyr Pro Asn Pro Ala Ala Tyr

1060 1065 1070

Arg Asp Gln Leu Ile Arg Phe Ser Ser Leu Asn His Lys Ala Tyr Tyr

1075 1080 1085

Lys Leu Ala Leu Lys Ala Ser Glu Leu Leu Glu Gln Thr Lys Leu Ser

1090 1095 1100

Glu Leu Arg Ala Arg Ile Ala Arg Ser Leu Ser Glu Leu Glu Met Phe

1105 1110 1115 1120

Thr Glu Glu Ser Lys Gly Leu Ser Met His Lys Arg Glu Ile Ala Ile

1125 1130 1135

Lys Glu Ser Met Glu Asp Leu Val Thr Ala Pro Leu Pro Val Glu Asp

1140 1145 1150

Ala Leu Ile Ser Leu Phe Asp Cys Ser Asp Thr Thr Val Gln Gln Arg

1155 1160 1165

Val Ile Glu Thr Tyr Ile Ala Arg Leu Tyr Gln Pro His Leu Val Lys

1170 1175 1180

Asp Ser Ile Lys Met Lys Trp Ile Glu Ser Gly Val Ile Ala Leu Trp

1185 1190 1195 1200

Glu Phe Pro Glu Gly His Phe Asp Ala Arg Asn Gly Gly Ala Val Leu

1205 1210 1215

Gly Asp Lys Arg Trp Gly Ala Met Val Ile Val Lys Ser Leu Glu Ser

1220 1225 1230

Leu Ser Met Ala Ile Arg Phe Ala Leu Lys Glu Thr Ser His Tyr Thr

1235 1240 1245

Ser Ser Glu Gly Asn Met Met His Ile Ala Leu Leu Gly Ala Asp Asn

1250 1255 1260

Lys Met His Ile Ile Gln Glu Ser Gly Asp Asp Ala Asp Arg Ile Ala

1265 1270 1275 1280

Lys Leu Pro Leu Ile Leu Lys Asp Asn Val Thr Asp Leu His Ala Ser

1285 1290 1295

Gly Val Lys Thr Ile Ser Phe Ile Val Gln Arg Asp Glu Ala Arg Met

1300 1305 1310

Thr Met Arg Arg Thr Phe Leu Trp Ser Asp Glu Lys Leu Ser Tyr Glu

1315 1320 1325

Glu Glu Pro Ile Leu Arg His Val Glu Pro Pro Leu Ser Ala Leu Leu

1330 1335 1340

Glu Leu Asp Lys Leu Lys Val Lys Gly Tyr Asn Glu Met Lys Tyr Thr

1345 1350 1355 1360

Pro Ser Arg Asp Arg Gln Trp His Ile Tyr Thr Leu Arg Asn Thr Glu

1365 1370 1375

Asn Pro Lys Met Leu His Arg Val Phe Phe Arg Thr Leu Val Arg Gln

1380 1385 1390

Pro Ser Val Ser Asn Lys Phe Ser Ser Gly Gln Ile Gly Asp Met Glu

1395 1400 1405

Val Gly Ser Ala Glu Glu Pro Leu Ser Phe Thr Ser Thr Ser Ile Leu

1410 1415 1420

Arg Ser Leu Met Thr Ala Ile Glu Glu Leu Glu Leu His Ala Ile Arg

1425 1430 1435 1440

Thr Gly His Ser His Met Tyr Leu His Val Leu Lys Glu Gln Lys Leu

1445 1450 1455

Leu Asp Leu Val Pro Val Ser Gly Asn Thr Val Leu Asp Val Gly Gln

1460 1465 1470

Asp Glu Ala Thr Ala Tyr Ser Leu Leu Lys Glu Met Ala Met Lys Ile

1475 1480 1485

His Glu Leu Val Gly Ala Arg Met His His Leu Ser Val Cys Gln Trp

1490 1495 1500

Glu Val Lys Leu Lys Leu Asp Cys Asp Gly Pro Ala Ser Gly Thr Trp

1505 1510 1515 1520

Arg Ile Val Thr Thr Asn Val Thr Ser His Thr Cys Thr Val Asp Ile

1525 1530 1535

Tyr Arg Glu Met Glu Asp Lys Glu Ser Arg Lys Leu Val Tyr His Pro

1540 1545 1550

Ala Thr Pro Ala Ala Gly Pro Leu His Gly Val Ala Leu Asn Asn Pro

1555 1560 1565

Tyr Gln Pro Leu Ser Val Ile Asp Leu Lys Arg Cys Ser Ala Arg Asn

1570 1575 1580

Asn Arg Thr Thr Tyr Cys Tyr Asp Phe Pro Leu Ala Phe Glu Thr Ala

1585 1590 1595 1600

Val Arg Lys Ser Trp Ser Ser Ser Thr Ser Gly Ala Ser Lys Gly Val

1605 1610 1615

Glu Asn Ala Gln Cys Tyr Val Lys Ala Thr Glu Leu Val Phe Ala Asp

1620 1625 1630

Lys His Gly Ser Trp Gly Thr Pro Leu Val Gln Met Asp Arg Pro Ala

1635 1640 1645

Gly Leu Asn Asp Ile Gly Met Val Ala Trp Thr Leu Lys Met Ser Thr

1650 1655 1660

Pro Glu Phe Pro Ser Gly Arg Glu Ile Ile Val Val Ala Asn Asp Ile

1665 1670 1675 1680

Thr Phe Arg Ala Gly Ser Phe Gly Pro Arg Glu Asp Ala Phe Phe Glu

1685 1690 1695

Ala Val Thr Asn Leu Ala Cys Glu Lys Lys Leu Pro Leu Ile Tyr Leu

1700 1705 1710

Ala Ala Asn Ser Gly Ala Arg Ile Gly Ile Ala Asp Glu Val Lys Ser

1715 1720 1725

Cys Phe Arg Val Gly Trp Ser Asp Asp Gly Ser Pro Glu Arg Gly Phe

1730 1735 1740

Gln Tyr Ile Tyr Leu Ser Glu Glu Asp Tyr Ala Arg Ile Gly Thr Ser

1745 1750 1755 1760

Val Ile Ala His Lys Met Gln Leu Asp Ser Gly Glu Ile Arg Trp Val

1765 1770 1775

Ile Asp Ser Val Val Gly Lys Glu Asp Gly Leu Gly Val Glu Asn Ile

1780 1785 1790

His Gly Ser Ala Ala Ile Ala Ser Ala Tyr Ser Arg Ala Tyr Lys Glu

1795 1800 1805

Thr Phe Thr Leu Thr Phe Val Thr Gly Arg Thr Val Gly Ile Gly Ala

1810 1815 1820

Tyr Leu Ala Arg Leu Gly Ile Arg Cys Ile Gln Arg Leu Asp Gln Pro

1825 1830 1835 1840

Ile Ile Leu Thr Gly Tyr Ser Ala Leu Asn Lys Leu Leu Gly Arg Glu

1845 1850 1855

Val Tyr Ser Ser His Met Gln Leu Gly Gly Pro Lys Ile Met Ala Thr

1860 1865 1870

Asn Gly Val Val His Leu Thr Val Ser Asp Asp Leu Glu Gly Val Ser

1875 1880 1885

Asn Ile Leu Arg Trp Leu Ser Tyr Val Pro Ala Tyr Ile Gly Gly Pro

1890 1895 1900

Leu Pro Val Thr Thr Pro Leu Asp Pro Pro Asp Arg Pro Val Ala Tyr

1905 1910 1915 1920

Ile Pro Glu Asn Ser Cys Asp Pro Arg Ala Ala Ile Arg Gly Val Asp

1925 1930 1935

Asp Ser Gln Gly Lys Trp Leu Gly Gly Met Phe Asp Lys Asp Ser Phe

1940 1945 1950

Val Glu Thr Phe Glu Gly Trp Ala Lys Thr Val Val Thr Gly Arg Ala

1955 1960 1965

Lys Leu Gly Gly Ile Pro Val Gly Val Ile Ala Val Glu Thr Gln Thr

1970 1975 1980

Met Met Gln Thr Ile Pro Ala Asp Pro Gly Gln Leu Asp Ser Arg Glu

1985 1990 1995 2000

Gln Ser Val Pro Arg Ala Gly Gln Val Gly Phe Pro Asp Ser Ala Thr

2005 2010 2015

Lys Thr Ala Gln Ala Leu Leu Asp Phe Asn Arg Glu Gly Leu Pro Leu

2020 2025 2030

Phe Ile Leu Ala Asn Trp Arg Gly Phe Ser Gly Gly Gln Arg Asp Leu

2035 2040 2045

Phe Glu Gly Ile Leu Gln Ala Gly Ser Thr Ile Val Glu Asn Leu Arg

2050 2055 2060

Thr Tyr Asn Gln Pro Ala Phe Val Tyr Ile Pro Met Ala Ala Glu Leu

2065 2070 2075 2080

Arg Gly Gly Ala Trp Val Val Val Asp Ser Lys Ile Asn Pro Asp Arg

2085 2090 2095

Ile Glu Cys Tyr Ala Glu Arg Thr Ala Lys Gly Asn Val Leu Glu Pro

2100 2105 2110

Gln Gly Leu Ile Glu Ile Lys Phe Arg Ser Glu Glu Leu Gln Asp Cys

2115 2120 2125

Met Ser Arg Leu Asp Pro Thr Leu Ile Asp Leu Lys Ala Lys Leu Glu

2130 2135 2140

Val Ala Asn Lys Asn Gly Ser Ala Asp Thr Lys Ser Leu Gln Glu Asn

2145 2150 2155 2160

Ile Glu Ala Arg Thr Lys Gln Leu Met Pro Leu Tyr Thr Gln Ile Ala

2165 2170 2175

Ile Arg Phe Ala Glu Leu His Asp Thr Ser Leu Arg Met Ala Ala Lys

2180 2185 2190

Gly Val Ile Lys Lys Val Val Asp Trp Glu Glu Ser Arg Ser Phe Phe

2195 2200 2205

Tyr Lys Arg Leu Arg Arg Arg Ile Ser Glu Asp Val Leu Ala Lys Glu

2210 2215 2220

Ile Arg Ala Val Ala Gly Glu Gln Phe Ser His Gln Pro Ala Ile Glu

2225 2230 2235 2240

Leu Ile Lys Lys Trp Tyr Ser Ala Ser His Ala Ala Glu Trp Asp Asp

2245 2250 2255

Asp Asp Ala Phe Val Ala Trp Met Asp Asn Pro Glu Asn Tyr Lys Asp

2260 2265 2270

Tyr Ile Gln Tyr Leu Lys Ala Gln Arg Val Ser Gln Ser Leu Ser Ser

2275 2280 2285

Leu Ser Asp Ser Ser Ser Asp Leu Gln Ala Leu Pro Gln Gly Leu Ser

2290 2295 2300

Met Leu Leu Asp Lys Met Asp Pro Ser Arg Arg Ala Gln Leu Val Glu

2305 2310 2315 2320

Glu Ile Arg Lys Val Leu Gly

2325

<210> 11

<211> 6984

<212> DNA

<213> 水稻(Oryza sativa嘉花1号ACCase-6030突变核苷酸序列)

<400> 11

atgacatcca cacatgtggc gacattggga gttggtgccc aggcacctcc tcgtcaccag 60

aaaaagtcag ctggcactgc atttgtatca tctgggtcat caagaccctc ataccgaaag 120

aatggtcagc gtactcggtc acttagggaa gaaagcaatg gaggagtgtc tgattccaaa 180

aagcttaacc actctattcg ccaaggtctt gctggcatca ttgacctccc aaatgacgca 240

gcttcagaag ttgatatttc acatggttcc gaagatccca gggggcctac ggtcccaggt 300

tcctaccaaa tgaatgggat tatcaatgaa acacataatg ggaggcatgc ttcagtctcc 360

aaggttgttg agttttgtac ggcacttggt ggcaaaacac caattcacag tgtattagtg 420

gccaacaatg gaatggcagc agctaagttc atgcggagtg tccgaacatg ggctaatgat 480

acttttggat cagagaaggc aattcagctg atagctatgg caactccgga ggatctgagg 540

ataaatgcag agcacatcag aattgccgat caatttgtag aggtacctgg tggaacaaac 600

aacaacaact atgcaaatgt ccaactcata gtggagatag cagagagaac aggtgtttct 660

gctgtttggc ctggttgggg tcatgcatct gagaatcctg aacttccaga tgcgctgact 720

gcaaaaggaa ttgtttttct tgggccacca gcatcatcaa tgcatgcatt aggagacaag 780

gttggctcag ctctcattgc tcaagcagct ggagttccaa cacttgcttg gagtggatca 840

catgtggaag ttcctctgga gtgttgcttg gactcaatac ctgatgagat gtatagaaaa 900

gcttgtgtta ctaccacaga ggaagcagtt gcaagttgtc aggtggttgg ttatcctgcc 960

atgattaagg catcttgggg tggtggtggt aaaggaataa ggaaggttca taatgatgat 1020

gaggttagga cattatttaa gcaagttcaa ggcgaagtac ctggttcccc aatatttatc 1080

atgaggctag ctgctcagag tcgacatctt gaagttcagt tgctttgtga tcaatatggc 1140

aacgtagcag cacttcacag tcgagattgc agtgtacaac ggcgacacca aaagataatc 1200

gaggaaggac cagttactgt tgctcctcgt gagactgtga aagagcttga gcaggcagca 1260

cggaggcttg ctaaagctgt gggttatgtt ggtgctgcta ctgttgaata cctttacagc 1320

atggaaactg gtgaatatta ttttctggaa cttaatccac ggctacaggt tgagcatcct 1380

gtcactgagt ggatagctga agtaaatttg cctgcggctc aagttgctgt tggaatgggt 1440

ataccccttt ggcagattcc agagatcagg cgcttctacg gaatgaacca tggaggaggc 1500

tatgaccttt ggaggaaaac agcagctcta gcgactccat ttaactttga tgaagtagat 1560

tctaaatggc caaaaggcca ctgcgtagct gttagaataa ctagcgagga tccagatgat 1620

gggtttaagc ctactggtgg aaaagtaaag gagataagtt tcaagagtaa accaaatgtt 1680

tgggcctatt tctcagtaaa gtctggtgga ggcatccatg aattcgctga ttctcagttc 1740

ggacatgttt ttgcgtatgg aactactaga tcggcagcaa taactaccat ggctcttgca 1800

ctaaaagagg ttcaaattcg tggagaaatt cattcaaacg tagactacac agttgaccta 1860

ttaaatgcct cagattttag agaaaataag attcatactg gttggctgga taccaggata 1920

gccatgcgtg ttcaagctga gaggcctcca tggtatattt cagtcgttgg aggggcttta 1980

tataaaacag taactgccaa cacggccact gtttctgatt atgttggtta tcttaccaag 2040

ggccagattc caccaaagca tatatccctt gtctatacga ctgttgcttt gaatatagat 2100

gggaaaaaat atacaatcga tactgtgagg agtggacatg gtagctacag attgcgaatg 2160

aatggatcaa cggttgacgc aaatgtacaa atattatgtg atggtgggct tttaatgcag 2220

ctggatggaa acagccatgt aatttatgct gaagaagagg ccagtggtac acgacttctt 2280

attgatggaa agacatgcat gttacagaat gaccatgacc catcaaagtt attagctgag 2340

acaccatgca aacttcttcg tttcttggtt gctgatggtg ctcatgttga tgctgatgta 2400

ccatatgcgg aagttgaggt tatgaagatg tgcatgcccc tcttatcacc cgcttctggt 2460

gtcatacatg ttgtaatgtc tgagggccaa gcaatgcagg ctggtgatct tatagctagg 2520

ctggatcttg atgacccttc tgctgttaag agagctgagc cgttcgaaga tacttttcca 2580

caaatgggtc tccctattgc tgcttctggc caagttcaca aattatgtgc tgcaagtctg 2640

aatgcttgtc gaatgatcct tgcggggtat gagcatgata ttgacaaggt tgtgccagag 2700

ttggtatact gcctagacac tccggagctt cctttcctgc agtgggagga gcttatgtct 2760

gttttagcaa ctagacttcc aagaaatctt aaaagtgagt tggagggcaa atatgaggaa 2820

tacaaagtaa aatttgactc tgggataatc aatgatttcc ctgccaatat gctacgagtg 2880

ataattgagg aaaatcttgc atgtggttct gagaaggaga aggctacaaa tgagaggctt 2940

gttgagcctc ttatgagcct actgaagtca tatgagggtg ggagagaaag tcatgctcac 3000

tttgttgtca agtccctttt tgaggagtat ctctatgttg aagaattgtt cagtgatgga 3060

attcagtctg atgtgattga gcgtctgcgc cttcaacata gtaaagacct acagaaggtc 3120

gtagacattg tgttgtccca ccagagtgtt agaaataaaa ctaagctgat actaaaactc 3180

atggagagtc tggtctatcc aaatcctgct gcctacaggg atcaattgat tcgcttttct 3240

tcccttaatc acaaagcgta ttacaagttg gcacttaaag ctagtgaact tcttgaacaa 3300

acaaaactta gtgagctccg tgcaagaata gcaaggagcc tttcagagct ggagatgttt 3360

actgaggaaa gcaagggtct ctccatgcat aagcgagaaa ttgccattaa ggagagcatg 3420

gaagatttag tcactgctcc actgccagtt gaagatgcgc tcatttcttt atttgattgt 3480

agtgatacaa ctgttcaaca gagagtgatt gagacttata tagctcgatt ataccagcct 3540

catcttgtaa aggacagtat caaaatgaaa tggatagaat cgggtgttat tgctttatgg 3600

gaatttcctg aagggcattt tgatgcaaga aatggaggag cggttcttgg tgacaaaaga 3660

tggggtgcca tggtcattgt caagtctctt gaatcacttt caatggccat tagatttgca 3720

ctaaaggaga catcacacta cactagctct gagggcaata tgatgcatat tgctttgttg 3780

ggtgctgata ataagatgca tataattcaa gaaagtggtg atgatgctga cagaatagcc 3840

aaacttccct tgatactaaa ggataatgta accgatctgc atgcctctgg tgtgaaaaca 3900

ataagtttca ttgttcaaag agatgaagca cggatgacaa tgcgtcgtac cttcctttgg 3960

tctgatgaaa agctttctta tgaggaagag ccaattctcc ggcatgtgga acctcctctt 4020

tctgcacttc ttgagttgga caagttgaaa gtgaaaggat acaatgaaat gaagtatacc 4080

ccatcacggg atcgtcaatg gcatatctac acacttagaa atactgaaaa ccccaaaatg 4140

ttgcaccggg tatttttccg aacccttgtc aggcaaccca gtgtatccaa caagttttct 4200

tcgggccaga ttggtgacat ggaagttggg agtgctgaag aacctctgtc atttacatca 4260

accagcatat taagatcttt gatgactgct atagaggaat tggagcttca cgcaattaga 4320

actggccatt cacacatgta tttgcatgta ttgaaagaac aaaagcttct tgatcttgtt 4380

ccagtttcag ggaatacagt tttggatgtt ggtcaagatg aagctactgc atattcactt 4440

ttaaaagaaa tggctatgaa gatacatgaa cttgttggtg caagaatgca ccatctttct 4500

gtatgccaat gggaagtgaa acttaagttg gactgcgatg gtcctgccag tggtacctgg 4560

aggattgtaa caaccaatgt tactagtcac acttgcactg tggatatcta ccgtgagatg 4620

gaagataaag aatcacggaa gttagtatac catcccgcca ctccggcggc tggtcctctg 4680

catggtgtgg cactgaataa tccatatcag cctttgagtg tcattgatct caaacgctgt 4740

tctgctagga ataatagaac tacatactgc tatgattttc cactggcatt tgaaactgca 4800

gtgaggaagt catggtcctc tagtacctct ggtgcttcta aaggtgttga aaatgcccaa 4860

tgttatgtta aagctacaga gttggtattt gcggacaaac atgggtcatg gggcactcct 4920

ttagttcaaa tggaccggcc tgctgggctc aatgacattg gtatggtagc ttggaccttg 4980

aagatgtcca ctcctgaatt tcctagtggt agggagatta ttgttgttgc aaatgatatt 5040

acgttcagag ctggatcatt tggcccaagg gaagatgcat tttttgaagc tgttaccaac 5100

ctagcctgtg agaagaaact tcctcttatt tatttggcag caaattctgg tgctcgaatt 5160

ggcatagcag atgaagtgaa atcttgcttc cgtgttgggt ggtctgatga tggcagccct 5220

gaacgtgggt ttcagtacat ttatctaagc gaagaagact atgctcgtat tggcacttct 5280

gtcatagcac ataagatgca gctagacagt ggtgaaatta ggtgggttat tgattctgtt 5340

gtgggcaagg aagatggact tggtgtggag aatatacatg gaagtgctgc tattgccagt 5400

gcttattcta gggcatataa ggagacattt acacttacat ttgtgactgg aagaactgtt 5460

ggaataggag cttatcttgc tcgacttggc atccggtgca tacagcgtct tgaccagcct 5520

attattctta caggctattc tgcactgaac aagcttcttg ggcgggaagt gtacagctcc 5580

cacatgcagt tgggtggtcc caaaatcatg gcaactaatg gtgttgtcca tcttactgtt 5640

tcagatgacc ttgaaggcgt ttctaatata ttgaggtggc tcagttatgt tcctgcctac 5700

attggtggac cacttccagt aacaacaccg ttggacccac cggacagacc tgttgcatac 5760

attcctgaga actcgtgtga tcctcgagcg gctatccgtg gtgttgatga cagccaaggg 5820

aaatggttag gtggtatgtt tgataaagac agctttgtgg aaacatttga aggttgggct 5880

aagacagtgg ttactggcag agcaaagctt ggtggaattc cagtgggtgt gatagctgtg 5940

gagactcaga ccatgatgca aactatccct gctgaccctg gtcagcttga ttcccgtgag 6000

caatctgttc ctcgtgctgg acaagtgtgt tttccagatt ctgcaaccaa gactgcgcag 6060

gcattgctgg acttcaaccg tgaaggatta cctctgttca tcctcgctaa ctggagaggc 6120

ttctctggtg gacaaagaga tctttttgaa ggaattcttc aggctggctc gactattgtt 6180

gagaacctta ggacatacaa tcagcctgcc tttgtctaca ttcccatggc tgcagagcta 6240

cgaggagggg cttgggttgt ggttgatagc aagataaacc cagaccgcat tgagtgctat 6300

gctgagagga ctgcaaaagg caatgttctg gaaccgcaag ggttaattga gatcaagttc 6360

aggtcagagg aactccagga ttgcatgagt cggcttgacc caacattaat tgatctgaaa 6420

gcaaaactcg aagtagcaaa taaaaatgga agtgctgaca caaaatcgct tcaagaaaat 6480

atagaagctc gaacaaaaca gttgatgcct ctatatactc agattgcgat acggtttgct 6540

gaattgcatg atacatccct cagaatggct gcgaaaggtg tgattaagaa agttgtggac 6600

tgggaagaat cacgatcttt cttctataag agattacgga ggaggatctc tgaggatgtt 6660

cttgcaaaag aaattagagc tgtagcaggt gagcagtttt cccaccaacc agcaatcgag 6720

ctgatcaaga aatggtattc agcttcacat gcagctgaat gggatgatga cgatgctttt 6780

gttgcttgga tggataaccc tgaaaactac aaggattata ttcaatatct taaggctcaa 6840

agagtatccc aatccctctc aagtctttca gattccagct cagatttgca agccctgcca 6900

cagggtcttt ccatgttact agataagatg gatccctcta gaagagctca acttgttgaa 6960

gaaatcagga aggtccttgg ttga 6984

<210> 12

<211> 2327

<212> PRT

<213> 水稻(Oryza sativa嘉花1号ACCase-6030突变氨基酸序列)

<400> 12

Met Thr Ser Thr His Val Ala Thr Leu Gly Val Gly Ala Gln Ala Pro

1 5 10 15

Pro Arg His Gln Lys Lys Ser Ala Gly Thr Ala Phe Val Ser Ser Gly

20 25 30

Ser Ser Arg Pro Ser Tyr Arg Lys Asn Gly Gln Arg Thr Arg Ser Leu

35 40 45

Arg Glu Glu Ser Asn Gly Gly Val Ser Asp Ser Lys Lys Leu Asn His

50 55 60

Ser Ile Arg Gln Gly Leu Ala Gly Ile Ile Asp Leu Pro Asn Asp Ala

65 70 75 80

Ala Ser Glu Val Asp Ile Ser His Gly Ser Glu Asp Pro Arg Gly Pro

85 90 95

Thr Val Pro Gly Ser Tyr Gln Met Asn Gly Ile Ile Asn Glu Thr His

100 105 110

Asn Gly Arg His Ala Ser Val Ser Lys Val Val Glu Phe Cys Thr Ala

115 120 125

Leu Gly Gly Lys Thr Pro Ile His Ser Val Leu Val Ala Asn Asn Gly

130 135 140

Met Ala Ala Ala Lys Phe Met Arg Ser Val Arg Thr Trp Ala Asn Asp

145 150 155 160

Thr Phe Gly Ser Glu Lys Ala Ile Gln Leu Ile Ala Met Ala Thr Pro

165 170 175

Glu Asp Leu Arg Ile Asn Ala Glu His Ile Arg Ile Ala Asp Gln Phe

180 185 190

Val Glu Val Pro Gly Gly Thr Asn Asn Asn Asn Tyr Ala Asn Val Gln

195 200 205

Leu Ile Val Glu Ile Ala Glu Arg Thr Gly Val Ser Ala Val Trp Pro

210 215 220

Gly Trp Gly His Ala Ser Glu Asn Pro Glu Leu Pro Asp Ala Leu Thr

225 230 235 240

Ala Lys Gly Ile Val Phe Leu Gly Pro Pro Ala Ser Ser Met His Ala

245 250 255

Leu Gly Asp Lys Val Gly Ser Ala Leu Ile Ala Gln Ala Ala Gly Val

260 265 270

Pro Thr Leu Ala Trp Ser Gly Ser His Val Glu Val Pro Leu Glu Cys

275 280 285

Cys Leu Asp Ser Ile Pro Asp Glu Met Tyr Arg Lys Ala Cys Val Thr

290 295 300

Thr Thr Glu Glu Ala Val Ala Ser Cys Gln Val Val Gly Tyr Pro Ala

305 310 315 320

Met Ile Lys Ala Ser Trp Gly Gly Gly Gly Lys Gly Ile Arg Lys Val

325 330 335

His Asn Asp Asp Glu Val Arg Thr Leu Phe Lys Gln Val Gln Gly Glu

340 345 350

Val Pro Gly Ser Pro Ile Phe Ile Met Arg Leu Ala Ala Gln Ser Arg

355 360 365

His Leu Glu Val Gln Leu Leu Cys Asp Gln Tyr Gly Asn Val Ala Ala

370 375 380

Leu His Ser Arg Asp Cys Ser Val Gln Arg Arg His Gln Lys Ile Ile

385 390 395 400

Glu Glu Gly Pro Val Thr Val Ala Pro Arg Glu Thr Val Lys Glu Leu

405 410 415

Glu Gln Ala Ala Arg Arg Leu Ala Lys Ala Val Gly Tyr Val Gly Ala

420 425 430

Ala Thr Val Glu Tyr Leu Tyr Ser Met Glu Thr Gly Glu Tyr Tyr Phe

435 440 445

Leu Glu Leu Asn Pro Arg Leu Gln Val Glu His Pro Val Thr Glu Trp

450 455 460

Ile Ala Glu Val Asn Leu Pro Ala Ala Gln Val Ala Val Gly Met Gly

465 470 475 480

Ile Pro Leu Trp Gln Ile Pro Glu Ile Arg Arg Phe Tyr Gly Met Asn

485 490 495

His Gly Gly Gly Tyr Asp Leu Trp Arg Lys Thr Ala Ala Leu Ala Thr

500 505 510

Pro Phe Asn Phe Asp Glu Val Asp Ser Lys Trp Pro Lys Gly His Cys

515 520 525

Val Ala Val Arg Ile Thr Ser Glu Asp Pro Asp Asp Gly Phe Lys Pro

530 535 540

Thr Gly Gly Lys Val Lys Glu Ile Ser Phe Lys Ser Lys Pro Asn Val

545 550 555 560

Trp Ala Tyr Phe Ser Val Lys Ser Gly Gly Gly Ile His Glu Phe Ala

565 570 575

Asp Ser Gln Phe Gly His Val Phe Ala Tyr Gly Thr Thr Arg Ser Ala

580 585 590

Ala Ile Thr Thr Met Ala Leu Ala Leu Lys Glu Val Gln Ile Arg Gly

595 600 605

Glu Ile His Ser Asn Val Asp Tyr Thr Val Asp Leu Leu Asn Ala Ser

610 615 620

Asp Phe Arg Glu Asn Lys Ile His Thr Gly Trp Leu Asp Thr Arg Ile

625 630 635 640

Ala Met Arg Val Gln Ala Glu Arg Pro Pro Trp Tyr Ile Ser Val Val

645 650 655

Gly Gly Ala Leu Tyr Lys Thr Val Thr Ala Asn Thr Ala Thr Val Ser

660 665 670

Asp Tyr Val Gly Tyr Leu Thr Lys Gly Gln Ile Pro Pro Lys His Ile

675 680 685

Ser Leu Val Tyr Thr Thr Val Ala Leu Asn Ile Asp Gly Lys Lys Tyr

690 695 700

Thr Ile Asp Thr Val Arg Ser Gly His Gly Ser Tyr Arg Leu Arg Met

705 710 715 720

Asn Gly Ser Thr Val Asp Ala Asn Val Gln Ile Leu Cys Asp Gly Gly

725 730 735

Leu Leu Met Gln Leu Asp Gly Asn Ser His Val Ile Tyr Ala Glu Glu

740 745 750

Glu Ala Ser Gly Thr Arg Leu Leu Ile Asp Gly Lys Thr Cys Met Leu

755 760 765

Gln Asn Asp His Asp Pro Ser Lys Leu Leu Ala Glu Thr Pro Cys Lys

770 775 780

Leu Leu Arg Phe Leu Val Ala Asp Gly Ala His Val Asp Ala Asp Val

785 790 795 800

Pro Tyr Ala Glu Val Glu Val Met Lys Met Cys Met Pro Leu Leu Ser

805 810 815

Pro Ala Ser Gly Val Ile His Val Val Met Ser Glu Gly Gln Ala Met

820 825 830

Gln Ala Gly Asp Leu Ile Ala Arg Leu Asp Leu Asp Asp Pro Ser Ala

835 840 845

Val Lys Arg Ala Glu Pro Phe Glu Asp Thr Phe Pro Gln Met Gly Leu

850 855 860

Pro Ile Ala Ala Ser Gly Gln Val His Lys Leu Cys Ala Ala Ser Leu

865 870 875 880

Asn Ala Cys Arg Met Ile Leu Ala Gly Tyr Glu His Asp Ile Asp Lys

885 890 895

Val Val Pro Glu Leu Val Tyr Cys Leu Asp Thr Pro Glu Leu Pro Phe

900 905 910

Leu Gln Trp Glu Glu Leu Met Ser Val Leu Ala Thr Arg Leu Pro Arg

915 920 925

Asn Leu Lys Ser Glu Leu Glu Gly Lys Tyr Glu Glu Tyr Lys Val Lys

930 935 940

Phe Asp Ser Gly Ile Ile Asn Asp Phe Pro Ala Asn Met Leu Arg Val

945 950 955 960

Ile Ile Glu Glu Asn Leu Ala Cys Gly Ser Glu Lys Glu Lys Ala Thr

965 970 975

Asn Glu Arg Leu Val Glu Pro Leu Met Ser Leu Leu Lys Ser Tyr Glu

980 985 990

Gly Gly Arg Glu Ser His Ala His Phe Val Val Lys Ser Leu Phe Glu

995 1000 1005

Glu Tyr Leu Tyr Val Glu Glu Leu Phe Ser Asp Gly Ile Gln Ser Asp

1010 1015 1020

Val Ile Glu Arg Leu Arg Leu Gln His Ser Lys Asp Leu Gln Lys Val

1025 1030 1035 1040

Val Asp Ile Val Leu Ser His Gln Ser Val Arg Asn Lys Thr Lys Leu

1045 1050 1055

Ile Leu Lys Leu Met Glu Ser Leu Val Tyr Pro Asn Pro Ala Ala Tyr

1060 1065 1070

Arg Asp Gln Leu Ile Arg Phe Ser Ser Leu Asn His Lys Ala Tyr Tyr

1075 1080 1085

Lys Leu Ala Leu Lys Ala Ser Glu Leu Leu Glu Gln Thr Lys Leu Ser

1090 1095 1100

Glu Leu Arg Ala Arg Ile Ala Arg Ser Leu Ser Glu Leu Glu Met Phe

1105 1110 1115 1120

Thr Glu Glu Ser Lys Gly Leu Ser Met His Lys Arg Glu Ile Ala Ile

1125 1130 1135

Lys Glu Ser Met Glu Asp Leu Val Thr Ala Pro Leu Pro Val Glu Asp

1140 1145 1150

Ala Leu Ile Ser Leu Phe Asp Cys Ser Asp Thr Thr Val Gln Gln Arg

1155 1160 1165

Val Ile Glu Thr Tyr Ile Ala Arg Leu Tyr Gln Pro His Leu Val Lys

1170 1175 1180

Asp Ser Ile Lys Met Lys Trp Ile Glu Ser Gly Val Ile Ala Leu Trp

1185 1190 1195 1200

Glu Phe Pro Glu Gly His Phe Asp Ala Arg Asn Gly Gly Ala Val Leu

1205 1210 1215

Gly Asp Lys Arg Trp Gly Ala Met Val Ile Val Lys Ser Leu Glu Ser

1220 1225 1230

Leu Ser Met Ala Ile Arg Phe Ala Leu Lys Glu Thr Ser His Tyr Thr

1235 1240 1245

Ser Ser Glu Gly Asn Met Met His Ile Ala Leu Leu Gly Ala Asp Asn

1250 1255 1260

Lys Met His Ile Ile Gln Glu Ser Gly Asp Asp Ala Asp Arg Ile Ala

1265 1270 1275 1280

Lys Leu Pro Leu Ile Leu Lys Asp Asn Val Thr Asp Leu His Ala Ser

1285 1290 1295

Gly Val Lys Thr Ile Ser Phe Ile Val Gln Arg Asp Glu Ala Arg Met

1300 1305 1310

Thr Met Arg Arg Thr Phe Leu Trp Ser Asp Glu Lys Leu Ser Tyr Glu

1315 1320 1325

Glu Glu Pro Ile Leu Arg His Val Glu Pro Pro Leu Ser Ala Leu Leu

1330 1335 1340

Glu Leu Asp Lys Leu Lys Val Lys Gly Tyr Asn Glu Met Lys Tyr Thr

1345 1350 1355 1360

Pro Ser Arg Asp Arg Gln Trp His Ile Tyr Thr Leu Arg Asn Thr Glu

1365 1370 1375

Asn Pro Lys Met Leu His Arg Val Phe Phe Arg Thr Leu Val Arg Gln

1380 1385 1390

Pro Ser Val Ser Asn Lys Phe Ser Ser Gly Gln Ile Gly Asp Met Glu

1395 1400 1405

Val Gly Ser Ala Glu Glu Pro Leu Ser Phe Thr Ser Thr Ser Ile Leu

1410 1415 1420

Arg Ser Leu Met Thr Ala Ile Glu Glu Leu Glu Leu His Ala Ile Arg

1425 1430 1435 1440

Thr Gly His Ser His Met Tyr Leu His Val Leu Lys Glu Gln Lys Leu

1445 1450 1455

Leu Asp Leu Val Pro Val Ser Gly Asn Thr Val Leu Asp Val Gly Gln

1460 1465 1470

Asp Glu Ala Thr Ala Tyr Ser Leu Leu Lys Glu Met Ala Met Lys Ile

1475 1480 1485

His Glu Leu Val Gly Ala Arg Met His His Leu Ser Val Cys Gln Trp

1490 1495 1500

Glu Val Lys Leu Lys Leu Asp Cys Asp Gly Pro Ala Ser Gly Thr Trp

1505 1510 1515 1520

Arg Ile Val Thr Thr Asn Val Thr Ser His Thr Cys Thr Val Asp Ile

1525 1530 1535

Tyr Arg Glu Met Glu Asp Lys Glu Ser Arg Lys Leu Val Tyr His Pro

1540 1545 1550

Ala Thr Pro Ala Ala Gly Pro Leu His Gly Val Ala Leu Asn Asn Pro

1555 1560 1565

Tyr Gln Pro Leu Ser Val Ile Asp Leu Lys Arg Cys Ser Ala Arg Asn

1570 1575 1580

Asn Arg Thr Thr Tyr Cys Tyr Asp Phe Pro Leu Ala Phe Glu Thr Ala

1585 1590 1595 1600

Val Arg Lys Ser Trp Ser Ser Ser Thr Ser Gly Ala Ser Lys Gly Val

1605 1610 1615

Glu Asn Ala Gln Cys Tyr Val Lys Ala Thr Glu Leu Val Phe Ala Asp

1620 1625 1630

Lys His Gly Ser Trp Gly Thr Pro Leu Val Gln Met Asp Arg Pro Ala

1635 1640 1645

Gly Leu Asn Asp Ile Gly Met Val Ala Trp Thr Leu Lys Met Ser Thr

1650 1655 1660

Pro Glu Phe Pro Ser Gly Arg Glu Ile Ile Val Val Ala Asn Asp Ile

1665 1670 1675 1680

Thr Phe Arg Ala Gly Ser Phe Gly Pro Arg Glu Asp Ala Phe Phe Glu

1685 1690 1695

Ala Val Thr Asn Leu Ala Cys Glu Lys Lys Leu Pro Leu Ile Tyr Leu

1700 1705 1710

Ala Ala Asn Ser Gly Ala Arg Ile Gly Ile Ala Asp Glu Val Lys Ser

1715 1720 1725

Cys Phe Arg Val Gly Trp Ser Asp Asp Gly Ser Pro Glu Arg Gly Phe

1730 1735 1740

Gln Tyr Ile Tyr Leu Ser Glu Glu Asp Tyr Ala Arg Ile Gly Thr Ser

1745 1750 1755 1760

Val Ile Ala His Lys Met Gln Leu Asp Ser Gly Glu Ile Arg Trp Val

1765 1770 1775

Ile Asp Ser Val Val Gly Lys Glu Asp Gly Leu Gly Val Glu Asn Ile

1780 1785 1790

His Gly Ser Ala Ala Ile Ala Ser Ala Tyr Ser Arg Ala Tyr Lys Glu

1795 1800 1805

Thr Phe Thr Leu Thr Phe Val Thr Gly Arg Thr Val Gly Ile Gly Ala

1810 1815 1820

Tyr Leu Ala Arg Leu Gly Ile Arg Cys Ile Gln Arg Leu Asp Gln Pro

1825 1830 1835 1840

Ile Ile Leu Thr Gly Tyr Ser Ala Leu Asn Lys Leu Leu Gly Arg Glu

1845 1850 1855

Val Tyr Ser Ser His Met Gln Leu Gly Gly Pro Lys Ile Met Ala Thr

1860 1865 1870

Asn Gly Val Val His Leu Thr Val Ser Asp Asp Leu Glu Gly Val Ser

1875 1880 1885

Asn Ile Leu Arg Trp Leu Ser Tyr Val Pro Ala Tyr Ile Gly Gly Pro

1890 1895 1900

Leu Pro Val Thr Thr Pro Leu Asp Pro Pro Asp Arg Pro Val Ala Tyr

1905 1910 1915 1920

Ile Pro Glu Asn Ser Cys Asp Pro Arg Ala Ala Ile Arg Gly Val Asp

1925 1930 1935

Asp Ser Gln Gly Lys Trp Leu Gly Gly Met Phe Asp Lys Asp Ser Phe

1940 1945 1950

Val Glu Thr Phe Glu Gly Trp Ala Lys Thr Val Val Thr Gly Arg Ala

1955 1960 1965

Lys Leu Gly Gly Ile Pro Val Gly Val Ile Ala Val Glu Thr Gln Thr

1970 1975 1980

Met Met Gln Thr Ile Pro Ala Asp Pro Gly Gln Leu Asp Ser Arg Glu

1985 1990 1995 2000

Gln Ser Val Pro Arg Ala Gly Gln Val Trp Phe Pro Asp Ser Ala Thr

2005 2010 2015

Lys Thr Ala Gln Ala Leu Leu Asp Phe Asn Arg Glu Gly Leu Pro Leu

2020 2025 2030

Phe Ile Leu Ala Asn Trp Arg Gly Phe Ser Gly Gly Gln Arg Asp Leu

2035 2040 2045

Phe Glu Gly Ile Leu Gln Ala Gly Ser Thr Ile Val Glu Asn Leu Arg

2050 2055 2060

Thr Tyr Asn Gln Pro Ala Phe Val Tyr Ile Pro Met Ala Ala Glu Leu

2065 2070 2075 2080

Arg Gly Gly Ala Trp Val Val Val Asp Ser Lys Ile Asn Pro Asp Arg

2085 2090 2095

Ile Glu Cys Tyr Ala Glu Arg Thr Ala Lys Gly Asn Val Leu Glu Pro

2100 2105 2110

Gln Gly Leu Ile Glu Ile Lys Phe Arg Ser Glu Glu Leu Gln Asp Cys

2115 2120 2125

Met Ser Arg Leu Asp Pro Thr Leu Ile Asp Leu Lys Ala Lys Leu Glu

2130 2135 2140

Val Ala Asn Lys Asn Gly Ser Ala Asp Thr Lys Ser Leu Gln Glu Asn

2145 2150 2155 2160

Ile Glu Ala Arg Thr Lys Gln Leu Met Pro Leu Tyr Thr Gln Ile Ala

2165 2170 2175

Ile Arg Phe Ala Glu Leu His Asp Thr Ser Leu Arg Met Ala Ala Lys

2180 2185 2190

Gly Val Ile Lys Lys Val Val Asp Trp Glu Glu Ser Arg Ser Phe Phe

2195 2200 2205

Tyr Lys Arg Leu Arg Arg Arg Ile Ser Glu Asp Val Leu Ala Lys Glu

2210 2215 2220

Ile Arg Ala Val Ala Gly Glu Gln Phe Ser His Gln Pro Ala Ile Glu

2225 2230 2235 2240

Leu Ile Lys Lys Trp Tyr Ser Ala Ser His Ala Ala Glu Trp Asp Asp

2245 2250 2255

Asp Asp Ala Phe Val Ala Trp Met Asp Asn Pro Glu Asn Tyr Lys Asp

2260 2265 2270

Tyr Ile Gln Tyr Leu Lys Ala Gln Arg Val Ser Gln Ser Leu Ser Ser

2275 2280 2285

Leu Ser Asp Ser Ser Ser Asp Leu Gln Ala Leu Pro Gln Gly Leu Ser

2290 2295 2300

Met Leu Leu Asp Lys Met Asp Pro Ser Arg Arg Ala Gln Leu Val Glu

2305 2310 2315 2320

Glu Ile Arg Lys Val Leu Gly

2325

<210> 13

<211> 6984

<212> DNA

<213> 水稻(Oryza sativa嘉花1号ACCase-6149突变核苷酸序列)

<400> 13

atgacatcca cacatgtggc gacattggga gttggtgccc aggcacctcc tcgtcaccag 60

aaaaagtcag ctggcactgc atttgtatca tctgggtcat caagaccctc ataccgaaag 120

aatggtcagc gtactcggtc acttagggaa gaaagcaatg gaggagtgtc tgattccaaa 180

aagcttaacc actctattcg ccaaggtctt gctggcatca ttgacctccc aaatgacgca 240

gcttcagaag ttgatatttc acatggttcc gaagatccca gggggcctac ggtcccaggt 300

tcctaccaaa tgaatgggat tatcaatgaa acacataatg ggaggcatgc ttcagtctcc 360

aaggttgttg agttttgtac ggcacttggt ggcaaaacac caattcacag tgtattagtg 420

gccaacaatg gaatggcagc agctaagttc atgcggagtg tccgaacatg ggctaatgat 480

acttttggat cagagaaggc aattcagctg atagctatgg caactccgga ggatctgagg 540

ataaatgcag agcacatcag aattgccgat caatttgtag aggtacctgg tggaacaaac 600

aacaacaact atgcaaatgt ccaactcata gtggagatag cagagagaac aggtgtttct 660

gctgtttggc ctggttgggg tcatgcatct gagaatcctg aacttccaga tgcgctgact 720

gcaaaaggaa ttgtttttct tgggccacca gcatcatcaa tgcatgcatt aggagacaag 780

gttggctcag ctctcattgc tcaagcagct ggagttccaa cacttgcttg gagtggatca 840

catgtggaag ttcctctgga gtgttgcttg gactcaatac ctgatgagat gtatagaaaa 900

gcttgtgtta ctaccacaga ggaagcagtt gcaagttgtc aggtggttgg ttatcctgcc 960

atgattaagg catcttgggg tggtggtggt aaaggaataa ggaaggttca taatgatgat 1020

gaggttagga cattatttaa gcaagttcaa ggcgaagtac ctggttcccc aatatttatc 1080

atgaggctag ctgctcagag tcgacatctt gaagttcagt tgctttgtga tcaatatggc 1140

aacgtagcag cacttcacag tcgagattgc agtgtacaac ggcgacacca aaagataatc 1200

gaggaaggac cagttactgt tgctcctcgt gagactgtga aagagcttga gcaggcagca 1260

cggaggcttg ctaaagctgt gggttatgtt ggtgctgcta ctgttgaata cctttacagc 1320

atggaaactg gtgaatatta ttttctggaa cttaatccac ggctacaggt tgagcatcct 1380

gtcactgagt ggatagctga agtaaatttg cctgcggctc aagttgctgt tggaatgggt 1440

ataccccttt ggcagattcc agagatcagg cgcttctacg gaatgaacca tggaggaggc 1500

tatgaccttt ggaggaaaac agcagctcta gcgactccat ttaactttga tgaagtagat 1560

tctaaatggc caaaaggcca ctgcgtagct gttagaataa ctagcgagga tccagatgat 1620

gggtttaagc ctactggtgg aaaagtaaag gagataagtt tcaagagtaa accaaatgtt 1680

tgggcctatt tctcagtaaa gtctggtgga ggcatccatg aattcgctga ttctcagttc 1740

ggacatgttt ttgcgtatgg aactactaga tcggcagcaa taactaccat ggctcttgca 1800

ctaaaagagg ttcaaattcg tggagaaatt cattcaaacg tagactacac agttgaccta 1860

ttaaatgcct cagattttag agaaaataag attcatactg gttggctgga taccaggata 1920

gccatgcgtg ttcaagctga gaggcctcca tggtatattt cagtcgttgg aggggcttta 1980

tataaaacag taactgccaa cacggccact gtttctgatt atgttggtta tcttaccaag 2040

ggccagattc caccaaagca tatatccctt gtctatacga ctgttgcttt gaatatagat 2100

gggaaaaaat atacaatcga tactgtgagg agtggacatg gtagctacag attgcgaatg 2160

aatggatcaa cggttgacgc aaatgtacaa atattatgtg atggtgggct tttaatgcag 2220

ctggatggaa acagccatgt aatttatgct gaagaagagg ccagtggtac acgacttctt 2280

attgatggaa agacatgcat gttacagaat gaccatgacc catcaaagtt attagctgag 2340

acaccatgca aacttcttcg tttcttggtt gctgatggtg ctcatgttga tgctgatgta 2400

ccatatgcgg aagttgaggt tatgaagatg tgcatgcccc tcttatcacc cgcttctggt 2460

gtcatacatg ttgtaatgtc tgagggccaa gcaatgcagg ctggtgatct tatagctagg 2520

ctggatcttg atgacccttc tgctgttaag agagctgagc cgttcgaaga tacttttcca 2580

caaatgggtc tccctattgc tgcttctggc caagttcaca aattatgtgc tgcaagtctg 2640

aatgcttgtc gaatgatcct tgcggggtat gagcatgata ttgacaaggt tgtgccagag 2700

ttggtatact gcctagacac tccggagctt cctttcctgc agtgggagga gcttatgtct 2760

gttttagcaa ctagacttcc aagaaatctt aaaagtgagt tggagggcaa atatgaggaa 2820

tacaaagtaa aatttgactc tgggataatc aatgatttcc ctgccaatat gctacgagtg 2880

ataattgagg aaaatcttgc atgtggttct gagaaggaga aggctacaaa tgagaggctt 2940

gttgagcctc ttatgagcct actgaagtca tatgagggtg ggagagaaag tcatgctcac 3000

tttgttgtca agtccctttt tgaggagtat ctctatgttg aagaattgtt cagtgatgga 3060

attcagtctg atgtgattga gcgtctgcgc cttcaacata gtaaagacct acagaaggtc 3120

gtagacattg tgttgtccca ccagagtgtt agaaataaaa ctaagctgat actaaaactc 3180

atggagagtc tggtctatcc aaatcctgct gcctacaggg atcaattgat tcgcttttct 3240

tcccttaatc acaaagcgta ttacaagttg gcacttaaag ctagtgaact tcttgaacaa 3300

acaaaactta gtgagctccg tgcaagaata gcaaggagcc tttcagagct ggagatgttt 3360

actgaggaaa gcaagggtct ctccatgcat aagcgagaaa ttgccattaa ggagagcatg 3420

gaagatttag tcactgctcc actgccagtt gaagatgcgc tcatttcttt atttgattgt 3480

agtgatacaa ctgttcaaca gagagtgatt gagacttata tagctcgatt ataccagcct 3540

catcttgtaa aggacagtat caaaatgaaa tggatagaat cgggtgttat tgctttatgg 3600

gaatttcctg aagggcattt tgatgcaaga aatggaggag cggttcttgg tgacaaaaga 3660

tggggtgcca tggtcattgt caagtctctt gaatcacttt caatggccat tagatttgca 3720

ctaaaggaga catcacacta cactagctct gagggcaata tgatgcatat tgctttgttg 3780

ggtgctgata ataagatgca tataattcaa gaaagtggtg atgatgctga cagaatagcc 3840

aaacttccct tgatactaaa ggataatgta accgatctgc atgcctctgg tgtgaaaaca 3900

ataagtttca ttgttcaaag agatgaagca cggatgacaa tgcgtcgtac cttcctttgg 3960

tctgatgaaa agctttctta tgaggaagag ccaattctcc ggcatgtgga acctcctctt 4020

tctgcacttc ttgagttgga caagttgaaa gtgaaaggat acaatgaaat gaagtatacc 4080

ccatcacggg atcgtcaatg gcatatctac acacttagaa atactgaaaa ccccaaaatg 4140

ttgcaccggg tatttttccg aacccttgtc aggcaaccca gtgtatccaa caagttttct 4200

tcgggccaga ttggtgacat ggaagttggg agtgctgaag aacctctgtc atttacatca 4260

accagcatat taagatcttt gatgactgct atagaggaat tggagcttca cgcaattaga 4320

actggccatt cacacatgta tttgcatgta ttgaaagaac aaaagcttct tgatcttgtt 4380

ccagtttcag ggaatacagt tttggatgtt ggtcaagatg aagctactgc atattcactt 4440

ttaaaagaaa tggctatgaa gatacatgaa cttgttggtg caagaatgca ccatctttct 4500

gtatgccaat gggaagtgaa acttaagttg gactgcgatg gtcctgccag tggtacctgg 4560

aggattgtaa caaccaatgt tactagtcac acttgcactg tggatatcta ccgtgagatg 4620

gaagataaag aatcacggaa gttagtatac catcccgcca ctccggcggc tggtcctctg 4680

catggtgtgg cactgaataa tccatatcag cctttgagtg tcattgatct caaacgctgt 4740

tctgctagga ataatagaac tacatactgc tatgattttc cactggcatt tgaaactgca 4800

gtgaggaagt catggtcctc tagtacctct ggtgcttcta aaggtgttga aaatgcccaa 4860

tgttatgtta aagctacaga gttggtattt gcggacaaac atgggtcatg gggcactcct 4920

ttagttcaaa tggaccggcc tgctgggctc aatgacattg gtatggtagc ttggaccttg 4980

aagatgtcca ctcctgaatt tcctagtggt agggagatta ttgttgttgc aaatgatatt 5040

acgttcagag ctggatcatt tggcccaagg gaagatgcat tttttgaagc tgttaccaac 5100

ctagcctgtg agaagaaact tcctcttatt tatttggcag caaattctgg tgctcgaatt 5160

ggcatagcag atgaagtgaa atcttgcttc cgtgttgggt ggtctgatga tggcagccct 5220

gaacgtgggt ttcagtacat ttatctaagc gaagaagact atgctcgtat tggcacttct 5280

gtcatagcac ataagatgca gctagacagt ggtgaaatta ggtgggttat tgattctgtt 5340

gtgggcaagg aagatggact tggtgtggag aatatacatg gaagtgctgc tattgccagt 5400

gcttattcta gggcatataa ggagacattt acacttacat ttgtgactgg aagaactgtt 5460

ggaataggag cttatcttgc tcgacttggc atccggtgca tacagcgtct tgaccagcct 5520

attattctta caggctattc tgcactgaac aagcttcttg ggcgggaagt gtacagctcc 5580

cacatgcagt tgggtggtcc caaaatcatg gcaactaatg gtgttgtcca tcttactgtt 5640

tcagatgacc ttgaaggcgt ttctaatata ttgaggtggc tcagttatgt tcctgcctac 5700

attggtggac cacttccagt aacaacaccg ttggacccac cggacagacc tgttgcatac 5760

attcctgaga actcgtgtga tcctcgagcg gctatccgtg gtgttgatga cagccaaggg 5820

aaatggttag gtggtatgtt tgataaagac agctttgtgg aaacatttga aggttgggct 5880

aagacagtgg ttactggcag agcaaagctt ggtggaattc cagtgggtgt gatagctgtg 5940

gagactcaga ccatgatgca aactatccct gctgaccctg gtcagcttga ttcccgtgag 6000

caatctgttc ctcgtgctgg acaagtgtgg tttccagatt ctgcaaccaa gactgcgcag 6060

gcattgctgg acttcaaccg tgaaggatta cctctgttca tcctcgctaa ctggagaggc 6120

ttctctggtg gacaaagaga tctttttgca ggaattcttc aggctggctc gactattgtt 6180

gagaacctta ggacatacaa tcagcctgcc tttgtctaca ttcccatggc tgcagagcta 6240

cgaggagggg cttgggttgt ggttgatagc aagataaacc cagaccgcat tgagtgctat 6300

gctgagagga ctgcaaaagg caatgttctg gaaccgcaag ggttaattga gatcaagttc 6360

aggtcagagg aactccagga ttgcatgagt cggcttgacc caacattaat tgatctgaaa 6420

gcaaaactcg aagtagcaaa taaaaatgga agtgctgaca caaaatcgct tcaagaaaat 6480

atagaagctc gaacaaaaca gttgatgcct ctatatactc agattgcgat acggtttgct 6540

gaattgcatg atacatccct cagaatggct gcgaaaggtg tgattaagaa agttgtggac 6600

tgggaagaat cacgatcttt cttctataag agattacgga ggaggatctc tgaggatgtt 6660

cttgcaaaag aaattagagc tgtagcaggt gagcagtttt cccaccaacc agcaatcgag 6720

ctgatcaaga aatggtattc agcttcacat gcagctgaat gggatgatga cgatgctttt 6780

gttgcttgga tggataaccc tgaaaactac aaggattata ttcaatatct taaggctcaa 6840

agagtatccc aatccctctc aagtctttca gattccagct cagatttgca agccctgcca 6900

cagggtcttt ccatgttact agataagatg gatccctcta gaagagctca acttgttgaa 6960

gaaatcagga aggtccttgg ttga 6984

<210> 14

<211> 2327

<212> PRT

<213> 水稻(Oryza sativa嘉花1号ACCase-6149突变氨基酸序列)

<400> 14

Met Thr Ser Thr His Val Ala Thr Leu Gly Val Gly Ala Gln Ala Pro

1 5 10 15

Pro Arg His Gln Lys Lys Ser Ala Gly Thr Ala Phe Val Ser Ser Gly

20 25 30

Ser Ser Arg Pro Ser Tyr Arg Lys Asn Gly Gln Arg Thr Arg Ser Leu

35 40 45

Arg Glu Glu Ser Asn Gly Gly Val Ser Asp Ser Lys Lys Leu Asn His

50 55 60

Ser Ile Arg Gln Gly Leu Ala Gly Ile Ile Asp Leu Pro Asn Asp Ala

65 70 75 80

Ala Ser Glu Val Asp Ile Ser His Gly Ser Glu Asp Pro Arg Gly Pro

85 90 95

Thr Val Pro Gly Ser Tyr Gln Met Asn Gly Ile Ile Asn Glu Thr His

100 105 110

Asn Gly Arg His Ala Ser Val Ser Lys Val Val Glu Phe Cys Thr Ala

115 120 125

Leu Gly Gly Lys Thr Pro Ile His Ser Val Leu Val Ala Asn Asn Gly

130 135 140

Met Ala Ala Ala Lys Phe Met Arg Ser Val Arg Thr Trp Ala Asn Asp

145 150 155 160

Thr Phe Gly Ser Glu Lys Ala Ile Gln Leu Ile Ala Met Ala Thr Pro

165 170 175

Glu Asp Leu Arg Ile Asn Ala Glu His Ile Arg Ile Ala Asp Gln Phe

180 185 190

Val Glu Val Pro Gly Gly Thr Asn Asn Asn Asn Tyr Ala Asn Val Gln

195 200 205

Leu Ile Val Glu Ile Ala Glu Arg Thr Gly Val Ser Ala Val Trp Pro

210 215 220

Gly Trp Gly His Ala Ser Glu Asn Pro Glu Leu Pro Asp Ala Leu Thr

225 230 235 240

Ala Lys Gly Ile Val Phe Leu Gly Pro Pro Ala Ser Ser Met His Ala

245 250 255

Leu Gly Asp Lys Val Gly Ser Ala Leu Ile Ala Gln Ala Ala Gly Val

260 265 270

Pro Thr Leu Ala Trp Ser Gly Ser His Val Glu Val Pro Leu Glu Cys

275 280 285

Cys Leu Asp Ser Ile Pro Asp Glu Met Tyr Arg Lys Ala Cys Val Thr

290 295 300

Thr Thr Glu Glu Ala Val Ala Ser Cys Gln Val Val Gly Tyr Pro Ala

305 310 315 320

Met Ile Lys Ala Ser Trp Gly Gly Gly Gly Lys Gly Ile Arg Lys Val

325 330 335

His Asn Asp Asp Glu Val Arg Thr Leu Phe Lys Gln Val Gln Gly Glu

340 345 350

Val Pro Gly Ser Pro Ile Phe Ile Met Arg Leu Ala Ala Gln Ser Arg

355 360 365

His Leu Glu Val Gln Leu Leu Cys Asp Gln Tyr Gly Asn Val Ala Ala

370 375 380

Leu His Ser Arg Asp Cys Ser Val Gln Arg Arg His Gln Lys Ile Ile

385 390 395 400

Glu Glu Gly Pro Val Thr Val Ala Pro Arg Glu Thr Val Lys Glu Leu

405 410 415

Glu Gln Ala Ala Arg Arg Leu Ala Lys Ala Val Gly Tyr Val Gly Ala

420 425 430

Ala Thr Val Glu Tyr Leu Tyr Ser Met Glu Thr Gly Glu Tyr Tyr Phe

435 440 445

Leu Glu Leu Asn Pro Arg Leu Gln Val Glu His Pro Val Thr Glu Trp

450 455 460

Ile Ala Glu Val Asn Leu Pro Ala Ala Gln Val Ala Val Gly Met Gly

465 470 475 480

Ile Pro Leu Trp Gln Ile Pro Glu Ile Arg Arg Phe Tyr Gly Met Asn

485 490 495

His Gly Gly Gly Tyr Asp Leu Trp Arg Lys Thr Ala Ala Leu Ala Thr

500 505 510

Pro Phe Asn Phe Asp Glu Val Asp Ser Lys Trp Pro Lys Gly His Cys

515 520 525

Val Ala Val Arg Ile Thr Ser Glu Asp Pro Asp Asp Gly Phe Lys Pro

530 535 540

Thr Gly Gly Lys Val Lys Glu Ile Ser Phe Lys Ser Lys Pro Asn Val

545 550 555 560

Trp Ala Tyr Phe Ser Val Lys Ser Gly Gly Gly Ile His Glu Phe Ala

565 570 575

Asp Ser Gln Phe Gly His Val Phe Ala Tyr Gly Thr Thr Arg Ser Ala

580 585 590

Ala Ile Thr Thr Met Ala Leu Ala Leu Lys Glu Val Gln Ile Arg Gly

595 600 605

Glu Ile His Ser Asn Val Asp Tyr Thr Val Asp Leu Leu Asn Ala Ser

610 615 620

Asp Phe Arg Glu Asn Lys Ile His Thr Gly Trp Leu Asp Thr Arg Ile

625 630 635 640

Ala Met Arg Val Gln Ala Glu Arg Pro Pro Trp Tyr Ile Ser Val Val

645 650 655

Gly Gly Ala Leu Tyr Lys Thr Val Thr Ala Asn Thr Ala Thr Val Ser

660 665 670

Asp Tyr Val Gly Tyr Leu Thr Lys Gly Gln Ile Pro Pro Lys His Ile

675 680 685

Ser Leu Val Tyr Thr Thr Val Ala Leu Asn Ile Asp Gly Lys Lys Tyr

690 695 700

Thr Ile Asp Thr Val Arg Ser Gly His Gly Ser Tyr Arg Leu Arg Met

705 710 715 720

Asn Gly Ser Thr Val Asp Ala Asn Val Gln Ile Leu Cys Asp Gly Gly

725 730 735

Leu Leu Met Gln Leu Asp Gly Asn Ser His Val Ile Tyr Ala Glu Glu

740 745 750

Glu Ala Ser Gly Thr Arg Leu Leu Ile Asp Gly Lys Thr Cys Met Leu

755 760 765

Gln Asn Asp His Asp Pro Ser Lys Leu Leu Ala Glu Thr Pro Cys Lys

770 775 780

Leu Leu Arg Phe Leu Val Ala Asp Gly Ala His Val Asp Ala Asp Val

785 790 795 800

Pro Tyr Ala Glu Val Glu Val Met Lys Met Cys Met Pro Leu Leu Ser

805 810 815

Pro Ala Ser Gly Val Ile His Val Val Met Ser Glu Gly Gln Ala Met

820 825 830

Gln Ala Gly Asp Leu Ile Ala Arg Leu Asp Leu Asp Asp Pro Ser Ala

835 840 845

Val Lys Arg Ala Glu Pro Phe Glu Asp Thr Phe Pro Gln Met Gly Leu

850 855 860

Pro Ile Ala Ala Ser Gly Gln Val His Lys Leu Cys Ala Ala Ser Leu

865 870 875 880

Asn Ala Cys Arg Met Ile Leu Ala Gly Tyr Glu His Asp Ile Asp Lys

885 890 895

Val Val Pro Glu Leu Val Tyr Cys Leu Asp Thr Pro Glu Leu Pro Phe

900 905 910

Leu Gln Trp Glu Glu Leu Met Ser Val Leu Ala Thr Arg Leu Pro Arg

915 920 925

Asn Leu Lys Ser Glu Leu Glu Gly Lys Tyr Glu Glu Tyr Lys Val Lys

930 935 940

Phe Asp Ser Gly Ile Ile Asn Asp Phe Pro Ala Asn Met Leu Arg Val

945 950 955 960

Ile Ile Glu Glu Asn Leu Ala Cys Gly Ser Glu Lys Glu Lys Ala Thr

965 970 975

Asn Glu Arg Leu Val Glu Pro Leu Met Ser Leu Leu Lys Ser Tyr Glu

980 985 990

Gly Gly Arg Glu Ser His Ala His Phe Val Val Lys Ser Leu Phe Glu

995 1000 1005

Glu Tyr Leu Tyr Val Glu Glu Leu Phe Ser Asp Gly Ile Gln Ser Asp

1010 1015 1020

Val Ile Glu Arg Leu Arg Leu Gln His Ser Lys Asp Leu Gln Lys Val

1025 1030 1035 1040

Val Asp Ile Val Leu Ser His Gln Ser Val Arg Asn Lys Thr Lys Leu

1045 1050 1055

Ile Leu Lys Leu Met Glu Ser Leu Val Tyr Pro Asn Pro Ala Ala Tyr

1060 1065 1070

Arg Asp Gln Leu Ile Arg Phe Ser Ser Leu Asn His Lys Ala Tyr Tyr

1075 1080 1085

Lys Leu Ala Leu Lys Ala Ser Glu Leu Leu Glu Gln Thr Lys Leu Ser

1090 1095 1100

Glu Leu Arg Ala Arg Ile Ala Arg Ser Leu Ser Glu Leu Glu Met Phe

1105 1110 1115 1120

Thr Glu Glu Ser Lys Gly Leu Ser Met His Lys Arg Glu Ile Ala Ile

1125 1130 1135

Lys Glu Ser Met Glu Asp Leu Val Thr Ala Pro Leu Pro Val Glu Asp

1140 1145 1150

Ala Leu Ile Ser Leu Phe Asp Cys Ser Asp Thr Thr Val Gln Gln Arg

1155 1160 1165

Val Ile Glu Thr Tyr Ile Ala Arg Leu Tyr Gln Pro His Leu Val Lys

1170 1175 1180

Asp Ser Ile Lys Met Lys Trp Ile Glu Ser Gly Val Ile Ala Leu Trp

1185 1190 1195 1200

Glu Phe Pro Glu Gly His Phe Asp Ala Arg Asn Gly Gly Ala Val Leu

1205 1210 1215

Gly Asp Lys Arg Trp Gly Ala Met Val Ile Val Lys Ser Leu Glu Ser

1220 1225 1230

Leu Ser Met Ala Ile Arg Phe Ala Leu Lys Glu Thr Ser His Tyr Thr

1235 1240 1245

Ser Ser Glu Gly Asn Met Met His Ile Ala Leu Leu Gly Ala Asp Asn

1250 1255 1260

Lys Met His Ile Ile Gln Glu Ser Gly Asp Asp Ala Asp Arg Ile Ala

1265 1270 1275 1280

Lys Leu Pro Leu Ile Leu Lys Asp Asn Val Thr Asp Leu His Ala Ser

1285 1290 1295

Gly Val Lys Thr Ile Ser Phe Ile Val Gln Arg Asp Glu Ala Arg Met

1300 1305 1310

Thr Met Arg Arg Thr Phe Leu Trp Ser Asp Glu Lys Leu Ser Tyr Glu

1315 1320 1325

Glu Glu Pro Ile Leu Arg His Val Glu Pro Pro Leu Ser Ala Leu Leu

1330 1335 1340

Glu Leu Asp Lys Leu Lys Val Lys Gly Tyr Asn Glu Met Lys Tyr Thr

1345 1350 1355 1360

Pro Ser Arg Asp Arg Gln Trp His Ile Tyr Thr Leu Arg Asn Thr Glu

1365 1370 1375

Asn Pro Lys Met Leu His Arg Val Phe Phe Arg Thr Leu Val Arg Gln

1380 1385 1390

Pro Ser Val Ser Asn Lys Phe Ser Ser Gly Gln Ile Gly Asp Met Glu

1395 1400 1405

Val Gly Ser Ala Glu Glu Pro Leu Ser Phe Thr Ser Thr Ser Ile Leu

1410 1415 1420

Arg Ser Leu Met Thr Ala Ile Glu Glu Leu Glu Leu His Ala Ile Arg

1425 1430 1435 1440

Thr Gly His Ser His Met Tyr Leu His Val Leu Lys Glu Gln Lys Leu

1445 1450 1455

Leu Asp Leu Val Pro Val Ser Gly Asn Thr Val Leu Asp Val Gly Gln

1460 1465 1470

Asp Glu Ala Thr Ala Tyr Ser Leu Leu Lys Glu Met Ala Met Lys Ile

1475 1480 1485

His Glu Leu Val Gly Ala Arg Met His His Leu Ser Val Cys Gln Trp

1490 1495 1500

Glu Val Lys Leu Lys Leu Asp Cys Asp Gly Pro Ala Ser Gly Thr Trp

1505 1510 1515 1520

Arg Ile Val Thr Thr Asn Val Thr Ser His Thr Cys Thr Val Asp Ile

1525 1530 1535

Tyr Arg Glu Met Glu Asp Lys Glu Ser Arg Lys Leu Val Tyr His Pro

1540 1545 1550

Ala Thr Pro Ala Ala Gly Pro Leu His Gly Val Ala Leu Asn Asn Pro

1555 1560 1565

Tyr Gln Pro Leu Ser Val Ile Asp Leu Lys Arg Cys Ser Ala Arg Asn

1570 1575 1580

Asn Arg Thr Thr Tyr Cys Tyr Asp Phe Pro Leu Ala Phe Glu Thr Ala

1585 1590 1595 1600

Val Arg Lys Ser Trp Ser Ser Ser Thr Ser Gly Ala Ser Lys Gly Val

1605 1610 1615

Glu Asn Ala Gln Cys Tyr Val Lys Ala Thr Glu Leu Val Phe Ala Asp

1620 1625 1630

Lys His Gly Ser Trp Gly Thr Pro Leu Val Gln Met Asp Arg Pro Ala

1635 1640 1645

Gly Leu Asn Asp Ile Gly Met Val Ala Trp Thr Leu Lys Met Ser Thr

1650 1655 1660

Pro Glu Phe Pro Ser Gly Arg Glu Ile Ile Val Val Ala Asn Asp Ile

1665 1670 1675 1680

Thr Phe Arg Ala Gly Ser Phe Gly Pro Arg Glu Asp Ala Phe Phe Glu

1685 1690 1695

Ala Val Thr Asn Leu Ala Cys Glu Lys Lys Leu Pro Leu Ile Tyr Leu

1700 1705 1710

Ala Ala Asn Ser Gly Ala Arg Ile Gly Ile Ala Asp Glu Val Lys Ser

1715 1720 1725

Cys Phe Arg Val Gly Trp Ser Asp Asp Gly Ser Pro Glu Arg Gly Phe

1730 1735 1740

Gln Tyr Ile Tyr Leu Ser Glu Glu Asp Tyr Ala Arg Ile Gly Thr Ser

1745 1750 1755 1760

Val Ile Ala His Lys Met Gln Leu Asp Ser Gly Glu Ile Arg Trp Val

1765 1770 1775

Ile Asp Ser Val Val Gly Lys Glu Asp Gly Leu Gly Val Glu Asn Ile

1780 1785 1790

His Gly Ser Ala Ala Ile Ala Ser Ala Tyr Ser Arg Ala Tyr Lys Glu

1795 1800 1805

Thr Phe Thr Leu Thr Phe Val Thr Gly Arg Thr Val Gly Ile Gly Ala

1810 1815 1820

Tyr Leu Ala Arg Leu Gly Ile Arg Cys Ile Gln Arg Leu Asp Gln Pro

1825 1830 1835 1840

Ile Ile Leu Thr Gly Tyr Ser Ala Leu Asn Lys Leu Leu Gly Arg Glu

1845 1850 1855

Val Tyr Ser Ser His Met Gln Leu Gly Gly Pro Lys Ile Met Ala Thr

1860 1865 1870

Asn Gly Val Val His Leu Thr Val Ser Asp Asp Leu Glu Gly Val Ser

1875 1880 1885

Asn Ile Leu Arg Trp Leu Ser Tyr Val Pro Ala Tyr Ile Gly Gly Pro

1890 1895 1900

Leu Pro Val Thr Thr Pro Leu Asp Pro Pro Asp Arg Pro Val Ala Tyr

1905 1910 1915 1920

Ile Pro Glu Asn Ser Cys Asp Pro Arg Ala Ala Ile Arg Gly Val Asp

1925 1930 1935

Asp Ser Gln Gly Lys Trp Leu Gly Gly Met Phe Asp Lys Asp Ser Phe

1940 1945 1950

Val Glu Thr Phe Glu Gly Trp Ala Lys Thr Val Val Thr Gly Arg Ala

1955 1960 1965

Lys Leu Gly Gly Ile Pro Val Gly Val Ile Ala Val Glu Thr Gln Thr

1970 1975 1980

Met Met Gln Thr Ile Pro Ala Asp Pro Gly Gln Leu Asp Ser Arg Glu

1985 1990 1995 2000

Gln Ser Val Pro Arg Ala Gly Gln Val Trp Phe Pro Asp Ser Ala Thr

2005 2010 2015

Lys Thr Ala Gln Ala Leu Leu Asp Phe Asn Arg Glu Gly Leu Pro Leu

2020 2025 2030

Phe Ile Leu Ala Asn Trp Arg Gly Phe Ser Gly Gly Gln Arg Asp Leu

2035 2040 2045

Phe Gly Gly Ile Leu Gln Ala Gly Ser Thr Ile Val Glu Asn Leu Arg

2050 2055 2060

Thr Tyr Asn Gln Pro Ala Phe Val Tyr Ile Pro Met Ala Ala Glu Leu

2065 2070 2075 2080

Arg Gly Gly Ala Trp Val Val Val Asp Ser Lys Ile Asn Pro Asp Arg

2085 2090 2095

Ile Glu Cys Tyr Ala Glu Arg Thr Ala Lys Gly Asn Val Leu Glu Pro

2100 2105 2110

Gln Gly Leu Ile Glu Ile Lys Phe Arg Ser Glu Glu Leu Gln Asp Cys

2115 2120 2125

Met Ser Arg Leu Asp Pro Thr Leu Ile Asp Leu Lys Ala Lys Leu Glu

2130 2135 2140

Val Ala Asn Lys Asn Gly Ser Ala Asp Thr Lys Ser Leu Gln Glu Asn

2145 2150 2155 2160

Ile Glu Ala Arg Thr Lys Gln Leu Met Pro Leu Tyr Thr Gln Ile Ala

2165 2170 2175

Ile Arg Phe Ala Glu Leu His Asp Thr Ser Leu Arg Met Ala Ala Lys

2180 2185 2190

Gly Val Ile Lys Lys Val Val Asp Trp Glu Glu Ser Arg Ser Phe Phe

2195 2200 2205

Tyr Lys Arg Leu Arg Arg Arg Ile Ser Glu Asp Val Leu Ala Lys Glu

2210 2215 2220

Ile Arg Ala Val Ala Gly Glu Gln Phe Ser His Gln Pro Ala Ile Glu

2225 2230 2235 2240

Leu Ile Lys Lys Trp Tyr Ser Ala Ser His Ala Ala Glu Trp Asp Asp

2245 2250 2255

Asp Asp Ala Phe Val Ala Trp Met Asp Asn Pro Glu Asn Tyr Lys Asp

2260 2265 2270

Tyr Ile Gln Tyr Leu Lys Ala Gln Arg Val Ser Gln Ser Leu Ser Ser

2275 2280 2285

Leu Ser Asp Ser Ser Ser Asp Leu Gln Ala Leu Pro Gln Gly Leu Ser

2290 2295 2300

Met Leu Leu Asp Lys Met Asp Pro Ser Arg Arg Ala Gln Leu Val Glu

2305 2310 2315 2320

Glu Ile Arg Lys Val Leu Gly

2325

<210> 15

<211> 6984

<212> DNA

<213> 水稻(Oryza sativa嘉花1号ACCase-6178突变核苷酸序列)

<400> 15

atgacatcca cacatgtggc gacattggga gttggtgccc aggcacctcc tcgtcaccag 60

aaaaagtcag ctggcactgc atttgtatca tctgggtcat caagaccctc ataccgaaag 120

aatggtcagc gtactcggtc acttagggaa gaaagcaatg gaggagtgtc tgattccaaa 180

aagcttaacc actctattcg ccaaggtctt gctggcatca ttgacctccc aaatgacgca 240

gcttcagaag ttgatatttc acatggttcc gaagatccca gggggcctac ggtcccaggt 300

tcctaccaaa tgaatgggat tatcaatgaa acacataatg ggaggcatgc ttcagtctcc 360

aaggttgttg agttttgtac ggcacttggt ggcaaaacac caattcacag tgtattagtg 420

gccaacaatg gaatggcagc agctaagttc atgcggagtg tccgaacatg ggctaatgat 480

acttttggat cagagaaggc aattcagctg atagctatgg caactccgga ggatctgagg 540

ataaatgcag agcacatcag aattgccgat caatttgtag aggtacctgg tggaacaaac 600

aacaacaact atgcaaatgt ccaactcata gtggagatag cagagagaac aggtgtttct 660

gctgtttggc ctggttgggg tcatgcatct gagaatcctg aacttccaga tgcgctgact 720

gcaaaaggaa ttgtttttct tgggccacca gcatcatcaa tgcatgcatt aggagacaag 780

gttggctcag ctctcattgc tcaagcagct ggagttccaa cacttgcttg gagtggatca 840

catgtggaag ttcctctgga gtgttgcttg gactcaatac ctgatgagat gtatagaaaa 900

gcttgtgtta ctaccacaga ggaagcagtt gcaagttgtc aggtggttgg ttatcctgcc 960

atgattaagg catcttgggg tggtggtggt aaaggaataa ggaaggttca taatgatgat 1020

gaggttagga cattatttaa gcaagttcaa ggcgaagtac ctggttcccc aatatttatc 1080

atgaggctag ctgctcagag tcgacatctt gaagttcagt tgctttgtga tcaatatggc 1140

aacgtagcag cacttcacag tcgagattgc agtgtacaac ggcgacacca aaagataatc 1200

gaggaaggac cagttactgt tgctcctcgt gagactgtga aagagcttga gcaggcagca 1260

cggaggcttg ctaaagctgt gggttatgtt ggtgctgcta ctgttgaata cctttacagc 1320

atggaaactg gtgaatatta ttttctggaa cttaatccac ggctacaggt tgagcatcct 1380

gtcactgagt ggatagctga agtaaatttg cctgcggctc aagttgctgt tggaatgggt 1440

ataccccttt ggcagattcc agagatcagg cgcttctacg gaatgaacca tggaggaggc 1500

tatgaccttt ggaggaaaac agcagctcta gcgactccat ttaactttga tgaagtagat 1560

tctaaatggc caaaaggcca ctgcgtagct gttagaataa ctagcgagga tccagatgat 1620

gggtttaagc ctactggtgg aaaagtaaag gagataagtt tcaagagtaa accaaatgtt 1680

tgggcctatt tctcagtaaa gtctggtgga ggcatccatg aattcgctga ttctcagttc 1740

ggacatgttt ttgcgtatgg aactactaga tcggcagcaa taactaccat ggctcttgca 1800

ctaaaagagg ttcaaattcg tggagaaatt cattcaaacg tagactacac agttgaccta 1860

ttaaatgcct cagattttag agaaaataag attcatactg gttggctgga taccaggata 1920

gccatgcgtg ttcaagctga gaggcctcca tggtatattt cagtcgttgg aggggcttta 1980

tataaaacag taactgccaa cacggccact gtttctgatt atgttggtta tcttaccaag 2040

ggccagattc caccaaagca tatatccctt gtctatacga ctgttgcttt gaatatagat 2100

gggaaaaaat atacaatcga tactgtgagg agtggacatg gtagctacag attgcgaatg 2160

aatggatcaa cggttgacgc aaatgtacaa atattatgtg atggtgggct tttaatgcag 2220

ctggatggaa acagccatgt aatttatgct gaagaagagg ccagtggtac acgacttctt 2280

attgatggaa agacatgcat gttacagaat gaccatgacc catcaaagtt attagctgag 2340

acaccatgca aacttcttcg tttcttggtt gctgatggtg ctcatgttga tgctgatgta 2400

ccatatgcgg aagttgaggt tatgaagatg tgcatgcccc tcttatcacc cgcttctggt 2460

gtcatacatg ttgtaatgtc tgagggccaa gcaatgcagg ctggtgatct tatagctagg 2520

ctggatcttg atgacccttc tgctgttaag agagctgagc cgttcgaaga tacttttcca 2580

caaatgggtc tccctattgc tgcttctggc caagttcaca aattatgtgc tgcaagtctg 2640

aatgcttgtc gaatgatcct tgcggggtat gagcatgata ttgacaaggt tgtgccagag 2700

ttggtatact gcctagacac tccggagctt cctttcctgc agtgggagga gcttatgtct 2760

gttttagcaa ctagacttcc aagaaatctt aaaagtgagt tggagggcaa atatgaggaa 2820

tacaaagtaa aatttgactc tgggataatc aatgatttcc ctgccaatat gctacgagtg 2880

ataattgagg aaaatcttgc atgtggttct gagaaggaga aggctacaaa tgagaggctt 2940

gttgagcctc ttatgagcct actgaagtca tatgagggtg ggagagaaag tcatgctcac 3000

tttgttgtca agtccctttt tgaggagtat ctctatgttg aagaattgtt cagtgatgga 3060

attcagtctg atgtgattga gcgtctgcgc cttcaacata gtaaagacct acagaaggtc 3120

gtagacattg tgttgtccca ccagagtgtt agaaataaaa ctaagctgat actaaaactc 3180

atggagagtc tggtctatcc aaatcctgct gcctacaggg atcaattgat tcgcttttct 3240

tcccttaatc acaaagcgta ttacaagttg gcacttaaag ctagtgaact tcttgaacaa 3300

acaaaactta gtgagctccg tgcaagaata gcaaggagcc tttcagagct ggagatgttt 3360

actgaggaaa gcaagggtct ctccatgcat aagcgagaaa ttgccattaa ggagagcatg 3420

gaagatttag tcactgctcc actgccagtt gaagatgcgc tcatttcttt atttgattgt 3480

agtgatacaa ctgttcaaca gagagtgatt gagacttata tagctcgatt ataccagcct 3540

catcttgtaa aggacagtat caaaatgaaa tggatagaat cgggtgttat tgctttatgg 3600

gaatttcctg aagggcattt tgatgcaaga aatggaggag cggttcttgg tgacaaaaga 3660

tggggtgcca tggtcattgt caagtctctt gaatcacttt caatggccat tagatttgca 3720

ctaaaggaga catcacacta cactagctct gagggcaata tgatgcatat tgctttgttg 3780

ggtgctgata ataagatgca tataattcaa gaaagtggtg atgatgctga cagaatagcc 3840

aaacttccct tgatactaaa ggataatgta accgatctgc atgcctctgg tgtgaaaaca 3900

ataagtttca ttgttcaaag agatgaagca cggatgacaa tgcgtcgtac cttcctttgg 3960

tctgatgaaa agctttctta tgaggaagag ccaattctcc ggcatgtgga acctcctctt 4020

tctgcacttc ttgagttgga caagttgaaa gtgaaaggat acaatgaaat gaagtatacc 4080

ccatcacggg atcgtcaatg gcatatctac acacttagaa atactgaaaa ccccaaaatg 4140

ttgcaccggg tatttttccg aacccttgtc aggcaaccca gtgtatccaa caagttttct 4200

tcgggccaga ttggtgacat ggaagttggg agtgctgaag aacctctgtc atttacatca 4260

accagcatat taagatcttt gatgactgct atagaggaat tggagcttca cgcaattaga 4320

actggccatt cacacatgta tttgcatgta ttgaaagaac aaaagcttct tgatcttgtt 4380

ccagtttcag ggaatacagt tttggatgtt ggtcaagatg aagctactgc atattcactt 4440

ttaaaagaaa tggctatgaa gatacatgaa cttgttggtg caagaatgca ccatctttct 4500

gtatgccaat gggaagtgaa acttaagttg gactgcgatg gtcctgccag tggtacctgg 4560

aggattgtaa caaccaatgt tactagtcac acttgcactg tggatatcta ccgtgagatg 4620

gaagataaag aatcacggaa gttagtatac catcccgcca ctccggcggc tggtcctctg 4680

catggtgtgg cactgaataa tccatatcag cctttgagtg tcattgatct caaacgctgt 4740

tctgctagga ataatagaac tacatactgc tatgattttc cactggcatt tgaaactgca 4800

gtgaggaagt catggtcctc tagtacctct ggtgcttcta aaggtgttga aaatgcccaa 4860

tgttatgtta aagctacaga gttggtattt gcggacaaac atgggtcatg gggcactcct 4920

ttagttcaaa tggaccggcc tgctgggctc aatgacattg gtatggtagc ttggaccttg 4980

aagatgtcca ctcctgaatt tcctagtggt agggagatta ttgttgttgc aaatgatatt 5040

acgttcagag ctggatcatt tggcccaagg gaagatgcat tttttgaagc tgttaccaac 5100

ctagcctgtg agaagaaact tcctcttatt tatttggcag caaattctgg tgctcgaatt 5160

ggcatagcag atgaagtgaa atcttgcttc cgtgttgggt ggtctgatga tggcagccct 5220

gaacgtgggt ttcagtacat ttatctaagc gaagaagact atgctcgtat tggcacttct 5280

gtcatagcac ataagatgca gctagacagt ggtgaaatta ggtgggttat tgattctgtt 5340

gtgggcaagg aagatggact tggtgtggag aatatacatg gaagtgctgc tattgccagt 5400

gcttattcta gggcatataa ggagacattt acacttacat ttgtgactgg aagaactgtt 5460

ggaataggag cttatcttgc tcgacttggc atccggtgca tacagcgtct tgaccagcct 5520

attattctta caggctattc tgcactgaac aagcttcttg ggcgggaagt gtacagctcc 5580

cacatgcagt tgggtggtcc caaaatcatg gcaactaatg gtgttgtcca tcttactgtt 5640

tcagatgacc ttgaaggcgt ttctaatata ttgaggtggc tcagttatgt tcctgcctac 5700

attggtggac cacttccagt aacaacaccg ttggacccac cggacagacc tgttgcatac 5760

attcctgaga actcgtgtga tcctcgagcg gctatccgtg gtgttgatga cagccaaggg 5820

aaatggttag gtggtatgtt tgataaagac agctttgtgg aaacatttga aggttgggct 5880

aagacagtgg ttactggcag agcaaagctt ggtggaattc cagtgggtgt gatagctgtg 5940

gagactcaga ccatgatgca aactatccct gctgaccctg gtcagcttga ttcccgtgag 6000

caatctgttc ctcgtgctgg acaagtgtgg tttccagatt ctgcaaccaa gactgcgcag 6060

gcattgctgg acttcaaccg tgaaggatta cctctgttca tcctcgctaa ctggagaggc 6120

ttctctggtg gacaaagaga tctttttgaa ggaattcttc aggctggctc gactattctt 6180

gagaacctta ggacatacaa tcagcctgcc tttgtctaca ttcccatggc tgcagagcta 6240

cgaggagggg cttgggttgt ggttgatagc aagataaacc cagaccgcat tgagtgctat 6300

gctgagagga ctgcaaaagg caatgttctg gaaccgcaag ggttaattga gatcaagttc 6360

aggtcagagg aactccagga ttgcatgagt cggcttgacc caacattaat tgatctgaaa 6420

gcaaaactcg aagtagcaaa taaaaatgga agtgctgaca caaaatcgct tcaagaaaat 6480

atagaagctc gaacaaaaca gttgatgcct ctatatactc agattgcgat acggtttgct 6540

gaattgcatg atacatccct cagaatggct gcgaaaggtg tgattaagaa agttgtggac 6600

tgggaagaat cacgatcttt cttctataag agattacgga ggaggatctc tgaggatgtt 6660

cttgcaaaag aaattagagc tgtagcaggt gagcagtttt cccaccaacc agcaatcgag 6720

ctgatcaaga aatggtattc agcttcacat gcagctgaat gggatgatga cgatgctttt 6780

gttgcttgga tggataaccc tgaaaactac aaggattata ttcaatatct taaggctcaa 6840

agagtatccc aatccctctc aagtctttca gattccagct cagatttgca agccctgcca 6900

cagggtcttt ccatgttact agataagatg gatccctcta gaagagctca acttgttgaa 6960

gaaatcagga aggtccttgg ttga 6984

<210> 16

<211> 2327

<212> PRT

<213> 水稻(Oryza sativa嘉花1号ACCase-6178突变氨基酸序列)

<400> 16

Met Thr Ser Thr His Val Ala Thr Leu Gly Val Gly Ala Gln Ala Pro

1 5 10 15

Pro Arg His Gln Lys Lys Ser Ala Gly Thr Ala Phe Val Ser Ser Gly

20 25 30

Ser Ser Arg Pro Ser Tyr Arg Lys Asn Gly Gln Arg Thr Arg Ser Leu

35 40 45

Arg Glu Glu Ser Asn Gly Gly Val Ser Asp Ser Lys Lys Leu Asn His

50 55 60

Ser Ile Arg Gln Gly Leu Ala Gly Ile Ile Asp Leu Pro Asn Asp Ala

65 70 75 80

Ala Ser Glu Val Asp Ile Ser His Gly Ser Glu Asp Pro Arg Gly Pro

85 90 95

Thr Val Pro Gly Ser Tyr Gln Met Asn Gly Ile Ile Asn Glu Thr His

100 105 110

Asn Gly Arg His Ala Ser Val Ser Lys Val Val Glu Phe Cys Thr Ala

115 120 125

Leu Gly Gly Lys Thr Pro Ile His Ser Val Leu Val Ala Asn Asn Gly

130 135 140

Met Ala Ala Ala Lys Phe Met Arg Ser Val Arg Thr Trp Ala Asn Asp

145 150 155 160

Thr Phe Gly Ser Glu Lys Ala Ile Gln Leu Ile Ala Met Ala Thr Pro

165 170 175

Glu Asp Leu Arg Ile Asn Ala Glu His Ile Arg Ile Ala Asp Gln Phe

180 185 190

Val Glu Val Pro Gly Gly Thr Asn Asn Asn Asn Tyr Ala Asn Val Gln

195 200 205

Leu Ile Val Glu Ile Ala Glu Arg Thr Gly Val Ser Ala Val Trp Pro

210 215 220

Gly Trp Gly His Ala Ser Glu Asn Pro Glu Leu Pro Asp Ala Leu Thr

225 230 235 240

Ala Lys Gly Ile Val Phe Leu Gly Pro Pro Ala Ser Ser Met His Ala

245 250 255

Leu Gly Asp Lys Val Gly Ser Ala Leu Ile Ala Gln Ala Ala Gly Val

260 265 270

Pro Thr Leu Ala Trp Ser Gly Ser His Val Glu Val Pro Leu Glu Cys

275 280 285

Cys Leu Asp Ser Ile Pro Asp Glu Met Tyr Arg Lys Ala Cys Val Thr

290 295 300

Thr Thr Glu Glu Ala Val Ala Ser Cys Gln Val Val Gly Tyr Pro Ala

305 310 315 320

Met Ile Lys Ala Ser Trp Gly Gly Gly Gly Lys Gly Ile Arg Lys Val

325 330 335

His Asn Asp Asp Glu Val Arg Thr Leu Phe Lys Gln Val Gln Gly Glu

340 345 350

Val Pro Gly Ser Pro Ile Phe Ile Met Arg Leu Ala Ala Gln Ser Arg

355 360 365

His Leu Glu Val Gln Leu Leu Cys Asp Gln Tyr Gly Asn Val Ala Ala

370 375 380

Leu His Ser Arg Asp Cys Ser Val Gln Arg Arg His Gln Lys Ile Ile

385 390 395 400

Glu Glu Gly Pro Val Thr Val Ala Pro Arg Glu Thr Val Lys Glu Leu

405 410 415

Glu Gln Ala Ala Arg Arg Leu Ala Lys Ala Val Gly Tyr Val Gly Ala

420 425 430

Ala Thr Val Glu Tyr Leu Tyr Ser Met Glu Thr Gly Glu Tyr Tyr Phe

435 440 445

Leu Glu Leu Asn Pro Arg Leu Gln Val Glu His Pro Val Thr Glu Trp

450 455 460

Ile Ala Glu Val Asn Leu Pro Ala Ala Gln Val Ala Val Gly Met Gly

465 470 475 480

Ile Pro Leu Trp Gln Ile Pro Glu Ile Arg Arg Phe Tyr Gly Met Asn

485 490 495

His Gly Gly Gly Tyr Asp Leu Trp Arg Lys Thr Ala Ala Leu Ala Thr

500 505 510

Pro Phe Asn Phe Asp Glu Val Asp Ser Lys Trp Pro Lys Gly His Cys

515 520 525

Val Ala Val Arg Ile Thr Ser Glu Asp Pro Asp Asp Gly Phe Lys Pro

530 535 540

Thr Gly Gly Lys Val Lys Glu Ile Ser Phe Lys Ser Lys Pro Asn Val

545 550 555 560

Trp Ala Tyr Phe Ser Val Lys Ser Gly Gly Gly Ile His Glu Phe Ala

565 570 575

Asp Ser Gln Phe Gly His Val Phe Ala Tyr Gly Thr Thr Arg Ser Ala

580 585 590

Ala Ile Thr Thr Met Ala Leu Ala Leu Lys Glu Val Gln Ile Arg Gly

595 600 605

Glu Ile His Ser Asn Val Asp Tyr Thr Val Asp Leu Leu Asn Ala Ser

610 615 620

Asp Phe Arg Glu Asn Lys Ile His Thr Gly Trp Leu Asp Thr Arg Ile

625 630 635 640

Ala Met Arg Val Gln Ala Glu Arg Pro Pro Trp Tyr Ile Ser Val Val

645 650 655

Gly Gly Ala Leu Tyr Lys Thr Val Thr Ala Asn Thr Ala Thr Val Ser

660 665 670

Asp Tyr Val Gly Tyr Leu Thr Lys Gly Gln Ile Pro Pro Lys His Ile

675 680 685

Ser Leu Val Tyr Thr Thr Val Ala Leu Asn Ile Asp Gly Lys Lys Tyr

690 695 700

Thr Ile Asp Thr Val Arg Ser Gly His Gly Ser Tyr Arg Leu Arg Met

705 710 715 720

Asn Gly Ser Thr Val Asp Ala Asn Val Gln Ile Leu Cys Asp Gly Gly

725 730 735

Leu Leu Met Gln Leu Asp Gly Asn Ser His Val Ile Tyr Ala Glu Glu

740 745 750

Glu Ala Ser Gly Thr Arg Leu Leu Ile Asp Gly Lys Thr Cys Met Leu

755 760 765

Gln Asn Asp His Asp Pro Ser Lys Leu Leu Ala Glu Thr Pro Cys Lys

770 775 780

Leu Leu Arg Phe Leu Val Ala Asp Gly Ala His Val Asp Ala Asp Val

785 790 795 800

Pro Tyr Ala Glu Val Glu Val Met Lys Met Cys Met Pro Leu Leu Ser

805 810 815

Pro Ala Ser Gly Val Ile His Val Val Met Ser Glu Gly Gln Ala Met

820 825 830

Gln Ala Gly Asp Leu Ile Ala Arg Leu Asp Leu Asp Asp Pro Ser Ala

835 840 845

Val Lys Arg Ala Glu Pro Phe Glu Asp Thr Phe Pro Gln Met Gly Leu

850 855 860

Pro Ile Ala Ala Ser Gly Gln Val His Lys Leu Cys Ala Ala Ser Leu

865 870 875 880

Asn Ala Cys Arg Met Ile Leu Ala Gly Tyr Glu His Asp Ile Asp Lys

885 890 895

Val Val Pro Glu Leu Val Tyr Cys Leu Asp Thr Pro Glu Leu Pro Phe

900 905 910

Leu Gln Trp Glu Glu Leu Met Ser Val Leu Ala Thr Arg Leu Pro Arg

915 920 925

Asn Leu Lys Ser Glu Leu Glu Gly Lys Tyr Glu Glu Tyr Lys Val Lys

930 935 940

Phe Asp Ser Gly Ile Ile Asn Asp Phe Pro Ala Asn Met Leu Arg Val

945 950 955 960

Ile Ile Glu Glu Asn Leu Ala Cys Gly Ser Glu Lys Glu Lys Ala Thr

965 970 975

Asn Glu Arg Leu Val Glu Pro Leu Met Ser Leu Leu Lys Ser Tyr Glu

980 985 990

Gly Gly Arg Glu Ser His Ala His Phe Val Val Lys Ser Leu Phe Glu

995 1000 1005

Glu Tyr Leu Tyr Val Glu Glu Leu Phe Ser Asp Gly Ile Gln Ser Asp

1010 1015 1020

Val Ile Glu Arg Leu Arg Leu Gln His Ser Lys Asp Leu Gln Lys Val

1025 1030 1035 1040

Val Asp Ile Val Leu Ser His Gln Ser Val Arg Asn Lys Thr Lys Leu

1045 1050 1055

Ile Leu Lys Leu Met Glu Ser Leu Val Tyr Pro Asn Pro Ala Ala Tyr

1060 1065 1070

Arg Asp Gln Leu Ile Arg Phe Ser Ser Leu Asn His Lys Ala Tyr Tyr

1075 1080 1085

Lys Leu Ala Leu Lys Ala Ser Glu Leu Leu Glu Gln Thr Lys Leu Ser

1090 1095 1100

Glu Leu Arg Ala Arg Ile Ala Arg Ser Leu Ser Glu Leu Glu Met Phe

1105 1110 1115 1120

Thr Glu Glu Ser Lys Gly Leu Ser Met His Lys Arg Glu Ile Ala Ile

1125 1130 1135

Lys Glu Ser Met Glu Asp Leu Val Thr Ala Pro Leu Pro Val Glu Asp

1140 1145 1150

Ala Leu Ile Ser Leu Phe Asp Cys Ser Asp Thr Thr Val Gln Gln Arg

1155 1160 1165

Val Ile Glu Thr Tyr Ile Ala Arg Leu Tyr Gln Pro His Leu Val Lys

1170 1175 1180

Asp Ser Ile Lys Met Lys Trp Ile Glu Ser Gly Val Ile Ala Leu Trp

1185 1190 1195 1200

Glu Phe Pro Glu Gly His Phe Asp Ala Arg Asn Gly Gly Ala Val Leu

1205 1210 1215

Gly Asp Lys Arg Trp Gly Ala Met Val Ile Val Lys Ser Leu Glu Ser

1220 1225 1230

Leu Ser Met Ala Ile Arg Phe Ala Leu Lys Glu Thr Ser His Tyr Thr

1235 1240 1245

Ser Ser Glu Gly Asn Met Met His Ile Ala Leu Leu Gly Ala Asp Asn

1250 1255 1260

Lys Met His Ile Ile Gln Glu Ser Gly Asp Asp Ala Asp Arg Ile Ala

1265 1270 1275 1280

Lys Leu Pro Leu Ile Leu Lys Asp Asn Val Thr Asp Leu His Ala Ser

1285 1290 1295

Gly Val Lys Thr Ile Ser Phe Ile Val Gln Arg Asp Glu Ala Arg Met

1300 1305 1310

Thr Met Arg Arg Thr Phe Leu Trp Ser Asp Glu Lys Leu Ser Tyr Glu

1315 1320 1325

Glu Glu Pro Ile Leu Arg His Val Glu Pro Pro Leu Ser Ala Leu Leu

1330 1335 1340

Glu Leu Asp Lys Leu Lys Val Lys Gly Tyr Asn Glu Met Lys Tyr Thr

1345 1350 1355 1360

Pro Ser Arg Asp Arg Gln Trp His Ile Tyr Thr Leu Arg Asn Thr Glu

1365 1370 1375

Asn Pro Lys Met Leu His Arg Val Phe Phe Arg Thr Leu Val Arg Gln

1380 1385 1390

Pro Ser Val Ser Asn Lys Phe Ser Ser Gly Gln Ile Gly Asp Met Glu

1395 1400 1405

Val Gly Ser Ala Glu Glu Pro Leu Ser Phe Thr Ser Thr Ser Ile Leu

1410 1415 1420

Arg Ser Leu Met Thr Ala Ile Glu Glu Leu Glu Leu His Ala Ile Arg

1425 1430 1435 1440

Thr Gly His Ser His Met Tyr Leu His Val Leu Lys Glu Gln Lys Leu

1445 1450 1455

Leu Asp Leu Val Pro Val Ser Gly Asn Thr Val Leu Asp Val Gly Gln

1460 1465 1470

Asp Glu Ala Thr Ala Tyr Ser Leu Leu Lys Glu Met Ala Met Lys Ile

1475 1480 1485

His Glu Leu Val Gly Ala Arg Met His His Leu Ser Val Cys Gln Trp

1490 1495 1500

Glu Val Lys Leu Lys Leu Asp Cys Asp Gly Pro Ala Ser Gly Thr Trp

1505 1510 1515 1520

Arg Ile Val Thr Thr Asn Val Thr Ser His Thr Cys Thr Val Asp Ile

1525 1530 1535

Tyr Arg Glu Met Glu Asp Lys Glu Ser Arg Lys Leu Val Tyr His Pro

1540 1545 1550

Ala Thr Pro Ala Ala Gly Pro Leu His Gly Val Ala Leu Asn Asn Pro

1555 1560 1565

Tyr Gln Pro Leu Ser Val Ile Asp Leu Lys Arg Cys Ser Ala Arg Asn

1570 1575 1580

Asn Arg Thr Thr Tyr Cys Tyr Asp Phe Pro Leu Ala Phe Glu Thr Ala

1585 1590 1595 1600

Val Arg Lys Ser Trp Ser Ser Ser Thr Ser Gly Ala Ser Lys Gly Val

1605 1610 1615

Glu Asn Ala Gln Cys Tyr Val Lys Ala Thr Glu Leu Val Phe Ala Asp

1620 1625 1630

Lys His Gly Ser Trp Gly Thr Pro Leu Val Gln Met Asp Arg Pro Ala

1635 1640 1645

Gly Leu Asn Asp Ile Gly Met Val Ala Trp Thr Leu Lys Met Ser Thr

1650 1655 1660

Pro Glu Phe Pro Ser Gly Arg Glu Ile Ile Val Val Ala Asn Asp Ile

1665 1670 1675 1680

Thr Phe Arg Ala Gly Ser Phe Gly Pro Arg Glu Asp Ala Phe Phe Glu

1685 1690 1695

Ala Val Thr Asn Leu Ala Cys Glu Lys Lys Leu Pro Leu Ile Tyr Leu

1700 1705 1710

Ala Ala Asn Ser Gly Ala Arg Ile Gly Ile Ala Asp Glu Val Lys Ser

1715 1720 1725

Cys Phe Arg Val Gly Trp Ser Asp Asp Gly Ser Pro Glu Arg Gly Phe

1730 1735 1740

Gln Tyr Ile Tyr Leu Ser Glu Glu Asp Tyr Ala Arg Ile Gly Thr Ser

1745 1750 1755 1760

Val Ile Ala His Lys Met Gln Leu Asp Ser Gly Glu Ile Arg Trp Val

1765 1770 1775

Ile Asp Ser Val Val Gly Lys Glu Asp Gly Leu Gly Val Glu Asn Ile

1780 1785 1790

His Gly Ser Ala Ala Ile Ala Ser Ala Tyr Ser Arg Ala Tyr Lys Glu

1795 1800 1805

Thr Phe Thr Leu Thr Phe Val Thr Gly Arg Thr Val Gly Ile Gly Ala

1810 1815 1820

Tyr Leu Ala Arg Leu Gly Ile Arg Cys Ile Gln Arg Leu Asp Gln Pro

1825 1830 1835 1840

Ile Ile Leu Thr Gly Tyr Ser Ala Leu Asn Lys Leu Leu Gly Arg Glu

1845 1850 1855

Val Tyr Ser Ser His Met Gln Leu Gly Gly Pro Lys Ile Met Ala Thr

1860 1865 1870

Asn Gly Val Val His Leu Thr Val Ser Asp Asp Leu Glu Gly Val Ser

1875 1880 1885

Asn Ile Leu Arg Trp Leu Ser Tyr Val Pro Ala Tyr Ile Gly Gly Pro

1890 1895 1900

Leu Pro Val Thr Thr Pro Leu Asp Pro Pro Asp Arg Pro Val Ala Tyr

1905 1910 1915 1920

Ile Pro Glu Asn Ser Cys Asp Pro Arg Ala Ala Ile Arg Gly Val Asp

1925 1930 1935

Asp Ser Gln Gly Lys Trp Leu Gly Gly Met Phe Asp Lys Asp Ser Phe

1940 1945 1950

Val Glu Thr Phe Glu Gly Trp Ala Lys Thr Val Val Thr Gly Arg Ala

1955 1960 1965

Lys Leu Gly Gly Ile Pro Val Gly Val Ile Ala Val Glu Thr Gln Thr

1970 1975 1980

Met Met Gln Thr Ile Pro Ala Asp Pro Gly Gln Leu Asp Ser Arg Glu

1985 1990 1995 2000

Gln Ser Val Pro Arg Ala Gly Gln Val Trp Phe Pro Asp Ser Ala Thr

2005 2010 2015

Lys Thr Ala Gln Ala Leu Leu Asp Phe Asn Arg Glu Gly Leu Pro Leu

2020 2025 2030

Phe Ile Leu Ala Asn Trp Arg Gly Phe Ser Gly Gly Gln Arg Asp Leu

2035 2040 2045

Phe Glu Gly Ile Leu Gln Ala Gly Ser Thr Ile Leu Glu Asn Leu Arg

2050 2055 2060

Thr Tyr Asn Gln Pro Ala Phe Val Tyr Ile Pro Met Ala Ala Glu Leu

2065 2070 2075 2080

Arg Gly Gly Ala Trp Val Val Val Asp Ser Lys Ile Asn Pro Asp Arg

2085 2090 2095

Ile Glu Cys Tyr Ala Glu Arg Thr Ala Lys Gly Asn Val Leu Glu Pro

2100 2105 2110

Gln Gly Leu Ile Glu Ile Lys Phe Arg Ser Glu Glu Leu Gln Asp Cys

2115 2120 2125

Met Ser Arg Leu Asp Pro Thr Leu Ile Asp Leu Lys Ala Lys Leu Glu

2130 2135 2140

Val Ala Asn Lys Asn Gly Ser Ala Asp Thr Lys Ser Leu Gln Glu Asn

2145 2150 2155 2160

Ile Glu Ala Arg Thr Lys Gln Leu Met Pro Leu Tyr Thr Gln Ile Ala

2165 2170 2175

Ile Arg Phe Ala Glu Leu His Asp Thr Ser Leu Arg Met Ala Ala Lys

2180 2185 2190

Gly Val Ile Lys Lys Val Val Asp Trp Glu Glu Ser Arg Ser Phe Phe

2195 2200 2205

Tyr Lys Arg Leu Arg Arg Arg Ile Ser Glu Asp Val Leu Ala Lys Glu

2210 2215 2220

Ile Arg Ala Val Ala Gly Glu Gln Phe Ser His Gln Pro Ala Ile Glu

2225 2230 2235 2240

Leu Ile Lys Lys Trp Tyr Ser Ala Ser His Ala Ala Glu Trp Asp Asp

2245 2250 2255

Asp Asp Ala Phe Val Ala Trp Met Asp Asn Pro Glu Asn Tyr Lys Asp

2260 2265 2270

Tyr Ile Gln Tyr Leu Lys Ala Gln Arg Val Ser Gln Ser Leu Ser Ser

2275 2280 2285

Leu Ser Asp Ser Ser Ser Asp Leu Gln Ala Leu Pro Gln Gly Leu Ser

2290 2295 2300

Met Leu Leu Asp Lys Met Asp Pro Ser Arg Arg Ala Gln Leu Val Glu

2305 2310 2315 2320

Glu Ile Arg Lys Val Leu Gly

2325

<210> 17

<211> 6984

<212> DNA

<213> 水稻(Oryza sativa嘉花1号ACCase-6209突变核苷酸序列)

<400> 17

atgacatcca cacatgtggc gacattggga gttggtgccc aggcacctcc tcgtcaccag 60

aaaaagtcag ctggcactgc atttgtatca tctgggtcat caagaccctc ataccgaaag 120

aatggtcagc gtactcggtc acttagggaa gaaagcaatg gaggagtgtc tgattccaaa 180

aagcttaacc actctattcg ccaaggtctt gctggcatca ttgacctccc aaatgacgca 240

gcttcagaag ttgatatttc acatggttcc gaagatccca gggggcctac ggtcccaggt 300

tcctaccaaa tgaatgggat tatcaatgaa acacataatg ggaggcatgc ttcagtctcc 360

aaggttgttg agttttgtac ggcacttggt ggcaaaacac caattcacag tgtattagtg 420

gccaacaatg gaatggcagc agctaagttc atgcggagtg tccgaacatg ggctaatgat 480

acttttggat cagagaaggc aattcagctg atagctatgg caactccgga ggatctgagg 540

ataaatgcag agcacatcag aattgccgat caatttgtag aggtacctgg tggaacaaac 600

aacaacaact atgcaaatgt ccaactcata gtggagatag cagagagaac aggtgtttct 660

gctgtttggc ctggttgggg tcatgcatct gagaatcctg aacttccaga tgcgctgact 720

gcaaaaggaa ttgtttttct tgggccacca gcatcatcaa tgcatgcatt aggagacaag 780

gttggctcag ctctcattgc tcaagcagct ggagttccaa cacttgcttg gagtggatca 840

catgtggaag ttcctctgga gtgttgcttg gactcaatac ctgatgagat gtatagaaaa 900

gcttgtgtta ctaccacaga ggaagcagtt gcaagttgtc aggtggttgg ttatcctgcc 960

atgattaagg catcttgggg tggtggtggt aaaggaataa ggaaggttca taatgatgat 1020

gaggttagga cattatttaa gcaagttcaa ggcgaagtac ctggttcccc aatatttatc 1080

atgaggctag ctgctcagag tcgacatctt gaagttcagt tgctttgtga tcaatatggc 1140

aacgtagcag cacttcacag tcgagattgc agtgtacaac ggcgacacca aaagataatc 1200

gaggaaggac cagttactgt tgctcctcgt gagactgtga aagagcttga gcaggcagca 1260

cggaggcttg ctaaagctgt gggttatgtt ggtgctgcta ctgttgaata cctttacagc 1320

atggaaactg gtgaatatta ttttctggaa cttaatccac ggctacaggt tgagcatcct 1380

gtcactgagt ggatagctga agtaaatttg cctgcggctc aagttgctgt tggaatgggt 1440

ataccccttt ggcagattcc agagatcagg cgcttctacg gaatgaacca tggaggaggc 1500

tatgaccttt ggaggaaaac agcagctcta gcgactccat ttaactttga tgaagtagat 1560

tctaaatggc caaaaggcca ctgcgtagct gttagaataa ctagcgagga tccagatgat 1620

gggtttaagc ctactggtgg aaaagtaaag gagataagtt tcaagagtaa accaaatgtt 1680

tgggcctatt tctcagtaaa gtctggtgga ggcatccatg aattcgctga ttctcagttc 1740

ggacatgttt ttgcgtatgg aactactaga tcggcagcaa taactaccat ggctcttgca 1800

ctaaaagagg ttcaaattcg tggagaaatt cattcaaacg tagactacac agttgaccta 1860

ttaaatgcct cagattttag agaaaataag attcatactg gttggctgga taccaggata 1920

gccatgcgtg ttcaagctga gaggcctcca tggtatattt cagtcgttgg aggggcttta 1980

tataaaacag taactgccaa cacggccact gtttctgatt atgttggtta tcttaccaag 2040

ggccagattc caccaaagca tatatccctt gtctatacga ctgttgcttt gaatatagat 2100

gggaaaaaat atacaatcga tactgtgagg agtggacatg gtagctacag attgcgaatg 2160

aatggatcaa cggttgacgc aaatgtacaa atattatgtg atggtgggct tttaatgcag 2220

ctggatggaa acagccatgt aatttatgct gaagaagagg ccagtggtac acgacttctt 2280

attgatggaa agacatgcat gttacagaat gaccatgacc catcaaagtt attagctgag 2340

acaccatgca aacttcttcg tttcttggtt gctgatggtg ctcatgttga tgctgatgta 2400

ccatatgcgg aagttgaggt tatgaagatg tgcatgcccc tcttatcacc cgcttctggt 2460

gtcatacatg ttgtaatgtc tgagggccaa gcaatgcagg ctggtgatct tatagctagg 2520

ctggatcttg atgacccttc tgctgttaag agagctgagc cgttcgaaga tacttttcca 2580

caaatgggtc tccctattgc tgcttctggc caagttcaca aattatgtgc tgcaagtctg 2640

aatgcttgtc gaatgatcct tgcggggtat gagcatgata ttgacaaggt tgtgccagag 2700

ttggtatact gcctagacac tccggagctt cctttcctgc agtgggagga gcttatgtct 2760

gttttagcaa ctagacttcc aagaaatctt aaaagtgagt tggagggcaa atatgaggaa 2820

tacaaagtaa aatttgactc tgggataatc aatgatttcc ctgccaatat gctacgagtg 2880

ataattgagg aaaatcttgc atgtggttct gagaaggaga aggctacaaa tgagaggctt 2940

gttgagcctc ttatgagcct actgaagtca tatgagggtg ggagagaaag tcatgctcac 3000

tttgttgtca agtccctttt tgaggagtat ctctatgttg aagaattgtt cagtgatgga 3060

attcagtctg atgtgattga gcgtctgcgc cttcaacata gtaaagacct acagaaggtc 3120

gtagacattg tgttgtccca ccagagtgtt agaaataaaa ctaagctgat actaaaactc 3180

atggagagtc tggtctatcc aaatcctgct gcctacaggg atcaattgat tcgcttttct 3240

tcccttaatc acaaagcgta ttacaagttg gcacttaaag ctagtgaact tcttgaacaa 3300

acaaaactta gtgagctccg tgcaagaata gcaaggagcc tttcagagct ggagatgttt 3360

actgaggaaa gcaagggtct ctccatgcat aagcgagaaa ttgccattaa ggagagcatg 3420

gaagatttag tcactgctcc actgccagtt gaagatgcgc tcatttcttt atttgattgt 3480

agtgatacaa ctgttcaaca gagagtgatt gagacttata tagctcgatt ataccagcct 3540

catcttgtaa aggacagtat caaaatgaaa tggatagaat cgggtgttat tgctttatgg 3600

gaatttcctg aagggcattt tgatgcaaga aatggaggag cggttcttgg tgacaaaaga 3660

tggggtgcca tggtcattgt caagtctctt gaatcacttt caatggccat tagatttgca 3720

ctaaaggaga catcacacta cactagctct gagggcaata tgatgcatat tgctttgttg 3780

ggtgctgata ataagatgca tataattcaa gaaagtggtg atgatgctga cagaatagcc 3840

aaacttccct tgatactaaa ggataatgta accgatctgc atgcctctgg tgtgaaaaca 3900

ataagtttca ttgttcaaag agatgaagca cggatgacaa tgcgtcgtac cttcctttgg 3960

tctgatgaaa agctttctta tgaggaagag ccaattctcc ggcatgtgga acctcctctt 4020

tctgcacttc ttgagttgga caagttgaaa gtgaaaggat acaatgaaat gaagtatacc 4080

ccatcacggg atcgtcaatg gcatatctac acacttagaa atactgaaaa ccccaaaatg 4140

ttgcaccggg tatttttccg aacccttgtc aggcaaccca gtgtatccaa caagttttct 4200

tcgggccaga ttggtgacat ggaagttggg agtgctgaag aacctctgtc atttacatca 4260

accagcatat taagatcttt gatgactgct atagaggaat tggagcttca cgcaattaga 4320

actggccatt cacacatgta tttgcatgta ttgaaagaac aaaagcttct tgatcttgtt 4380

ccagtttcag ggaatacagt tttggatgtt ggtcaagatg aagctactgc atattcactt 4440

ttaaaagaaa tggctatgaa gatacatgaa cttgttggtg caagaatgca ccatctttct 4500

gtatgccaat gggaagtgaa acttaagttg gactgcgatg gtcctgccag tggtacctgg 4560

aggattgtaa caaccaatgt tactagtcac acttgcactg tggatatcta ccgtgagatg 4620

gaagataaag aatcacggaa gttagtatac catcccgcca ctccggcggc tggtcctctg 4680

catggtgtgg cactgaataa tccatatcag cctttgagtg tcattgatct caaacgctgt 4740

tctgctagga ataatagaac tacatactgc tatgattttc cactggcatt tgaaactgca 4800

gtgaggaagt catggtcctc tagtacctct ggtgcttcta aaggtgttga aaatgcccaa 4860

tgttatgtta aagctacaga gttggtattt gcggacaaac atgggtcatg gggcactcct 4920

ttagttcaaa tggaccggcc tgctgggctc aatgacattg gtatggtagc ttggaccttg 4980

aagatgtcca ctcctgaatt tcctagtggt agggagatta ttgttgttgc aaatgatatt 5040

acgttcagag ctggatcatt tggcccaagg gaagatgcat tttttgaagc tgttaccaac 5100

ctagcctgtg agaagaaact tcctcttatt tatttggcag caaattctgg tgctcgaatt 5160

ggcatagcag atgaagtgaa atcttgcttc cgtgttgggt ggtctgatga tggcagccct 5220

gaacgtgggt ttcagtacat ttatctaagc gaagaagact atgctcgtat tggcacttct 5280

gtcatagcac ataagatgca gctagacagt ggtgaaatta ggtgggttat tgattctgtt 5340

gtgggcaagg aagatggact tggtgtggag aatatacatg gaagtgctgc tattgccagt 5400

gcttattcta gggcatataa ggagacattt acacttacat ttgtgactgg aagaactgtt 5460

ggaataggag cttatcttgc tcgacttggc atccggtgca tacagcgtct tgaccagcct 5520

attattctta caggctattc tgcactgaac aagcttcttg ggcgggaagt gtacagctcc 5580

cacatgcagt tgggtggtcc caaaatcatg gcaactaatg gtgttgtcca tcttactgtt 5640

tcagatgacc ttgaaggcgt ttctaatata ttgaggtggc tcagttatgt tcctgcctac 5700

attggtggac cacttccagt aacaacaccg ttggacccac cggacagacc tgttgcatac 5760

attcctgaga actcgtgtga tcctcgagcg gctatccgtg gtgttgatga cagccaaggg 5820

aaatggttag gtggtatgtt tgataaagac agctttgtgg aaacatttga aggttgggct 5880

aagacagtgg ttactggcag agcaaagctt ggtggaattc cagtgggtgt gatagctgtg 5940

gagactcaga ccatgatgca aactatccct gctgaccctg gtcagcttga ttcccgtgag 6000

caatctgttc ctcgtgctgg acaagtgtgg tttccagatt ctgcaaccaa gactgcgcag 6060

gcattgctgg acttcaaccg tgaaggatta cctctgttca tcctcgctaa ctggagaggc 6120

ttctctggtg gacaaagaga tctttttgaa ggaattcttc aggctggctc gactattgtt 6180

gagaacctta ggacatacaa tcagcctgtc tttgtctaca ttcccatggc tgcagagcta 6240

cgaggagggg cttgggttgt ggttgatagc aagataaacc cagaccgcat tgagtgctat 6300

gctgagagga ctgcaaaagg caatgttctg gaaccgcaag ggttaattga gatcaagttc 6360

aggtcagagg aactccagga ttgcatgagt cggcttgacc caacattaat tgatctgaaa 6420

gcaaaactcg aagtagcaaa taaaaatgga agtgctgaca caaaatcgct tcaagaaaat 6480

atagaagctc gaacaaaaca gttgatgcct ctatatactc agattgcgat acggtttgct 6540

gaattgcatg atacatccct cagaatggct gcgaaaggtg tgattaagaa agttgtggac 6600

tgggaagaat cacgatcttt cttctataag agattacgga ggaggatctc tgaggatgtt 6660

cttgcaaaag aaattagagc tgtagcaggt gagcagtttt cccaccaacc agcaatcgag 6720

ctgatcaaga aatggtattc agcttcacat gcagctgaat gggatgatga cgatgctttt 6780

gttgcttgga tggataaccc tgaaaactac aaggattata ttcaatatct taaggctcaa 6840

agagtatccc aatccctctc aagtctttca gattccagct cagatttgca agccctgcca 6900

cagggtcttt ccatgttact agataagatg gatccctcta gaagagctca acttgttgaa 6960

gaaatcagga aggtccttgg ttga 6984

<210> 18

<211> 2327

<212> PRT

<213> 水稻(Oryza sativa嘉花1号ACCase-6209突变氨基酸序列)

<400> 18

Met Thr Ser Thr His Val Ala Thr Leu Gly Val Gly Ala Gln Ala Pro

1 5 10 15

Pro Arg His Gln Lys Lys Ser Ala Gly Thr Ala Phe Val Ser Ser Gly

20 25 30

Ser Ser Arg Pro Ser Tyr Arg Lys Asn Gly Gln Arg Thr Arg Ser Leu

35 40 45

Arg Glu Glu Ser Asn Gly Gly Val Ser Asp Ser Lys Lys Leu Asn His

50 55 60

Ser Ile Arg Gln Gly Leu Ala Gly Ile Ile Asp Leu Pro Asn Asp Ala

65 70 75 80

Ala Ser Glu Val Asp Ile Ser His Gly Ser Glu Asp Pro Arg Gly Pro

85 90 95

Thr Val Pro Gly Ser Tyr Gln Met Asn Gly Ile Ile Asn Glu Thr His

100 105 110

Asn Gly Arg His Ala Ser Val Ser Lys Val Val Glu Phe Cys Thr Ala

115 120 125

Leu Gly Gly Lys Thr Pro Ile His Ser Val Leu Val Ala Asn Asn Gly

130 135 140

Met Ala Ala Ala Lys Phe Met Arg Ser Val Arg Thr Trp Ala Asn Asp

145 150 155 160

Thr Phe Gly Ser Glu Lys Ala Ile Gln Leu Ile Ala Met Ala Thr Pro

165 170 175

Glu Asp Leu Arg Ile Asn Ala Glu His Ile Arg Ile Ala Asp Gln Phe

180 185 190

Val Glu Val Pro Gly Gly Thr Asn Asn Asn Asn Tyr Ala Asn Val Gln

195 200 205

Leu Ile Val Glu Ile Ala Glu Arg Thr Gly Val Ser Ala Val Trp Pro

210 215 220

Gly Trp Gly His Ala Ser Glu Asn Pro Glu Leu Pro Asp Ala Leu Thr

225 230 235 240

Ala Lys Gly Ile Val Phe Leu Gly Pro Pro Ala Ser Ser Met His Ala

245 250 255

Leu Gly Asp Lys Val Gly Ser Ala Leu Ile Ala Gln Ala Ala Gly Val

260 265 270

Pro Thr Leu Ala Trp Ser Gly Ser His Val Glu Val Pro Leu Glu Cys

275 280 285

Cys Leu Asp Ser Ile Pro Asp Glu Met Tyr Arg Lys Ala Cys Val Thr

290 295 300

Thr Thr Glu Glu Ala Val Ala Ser Cys Gln Val Val Gly Tyr Pro Ala

305 310 315 320

Met Ile Lys Ala Ser Trp Gly Gly Gly Gly Lys Gly Ile Arg Lys Val

325 330 335

His Asn Asp Asp Glu Val Arg Thr Leu Phe Lys Gln Val Gln Gly Glu

340 345 350

Val Pro Gly Ser Pro Ile Phe Ile Met Arg Leu Ala Ala Gln Ser Arg

355 360 365

His Leu Glu Val Gln Leu Leu Cys Asp Gln Tyr Gly Asn Val Ala Ala

370 375 380

Leu His Ser Arg Asp Cys Ser Val Gln Arg Arg His Gln Lys Ile Ile

385 390 395 400

Glu Glu Gly Pro Val Thr Val Ala Pro Arg Glu Thr Val Lys Glu Leu

405 410 415

Glu Gln Ala Ala Arg Arg Leu Ala Lys Ala Val Gly Tyr Val Gly Ala

420 425 430

Ala Thr Val Glu Tyr Leu Tyr Ser Met Glu Thr Gly Glu Tyr Tyr Phe

435 440 445

Leu Glu Leu Asn Pro Arg Leu Gln Val Glu His Pro Val Thr Glu Trp

450 455 460

Ile Ala Glu Val Asn Leu Pro Ala Ala Gln Val Ala Val Gly Met Gly

465 470 475 480

Ile Pro Leu Trp Gln Ile Pro Glu Ile Arg Arg Phe Tyr Gly Met Asn

485 490 495

His Gly Gly Gly Tyr Asp Leu Trp Arg Lys Thr Ala Ala Leu Ala Thr

500 505 510

Pro Phe Asn Phe Asp Glu Val Asp Ser Lys Trp Pro Lys Gly His Cys

515 520 525

Val Ala Val Arg Ile Thr Ser Glu Asp Pro Asp Asp Gly Phe Lys Pro

530 535 540

Thr Gly Gly Lys Val Lys Glu Ile Ser Phe Lys Ser Lys Pro Asn Val

545 550 555 560

Trp Ala Tyr Phe Ser Val Lys Ser Gly Gly Gly Ile His Glu Phe Ala

565 570 575

Asp Ser Gln Phe Gly His Val Phe Ala Tyr Gly Thr Thr Arg Ser Ala

580 585 590

Ala Ile Thr Thr Met Ala Leu Ala Leu Lys Glu Val Gln Ile Arg Gly

595 600 605

Glu Ile His Ser Asn Val Asp Tyr Thr Val Asp Leu Leu Asn Ala Ser

610 615 620

Asp Phe Arg Glu Asn Lys Ile His Thr Gly Trp Leu Asp Thr Arg Ile

625 630 635 640

Ala Met Arg Val Gln Ala Glu Arg Pro Pro Trp Tyr Ile Ser Val Val

645 650 655

Gly Gly Ala Leu Tyr Lys Thr Val Thr Ala Asn Thr Ala Thr Val Ser

660 665 670

Asp Tyr Val Gly Tyr Leu Thr Lys Gly Gln Ile Pro Pro Lys His Ile

675 680 685

Ser Leu Val Tyr Thr Thr Val Ala Leu Asn Ile Asp Gly Lys Lys Tyr

690 695 700

Thr Ile Asp Thr Val Arg Ser Gly His Gly Ser Tyr Arg Leu Arg Met

705 710 715 720

Asn Gly Ser Thr Val Asp Ala Asn Val Gln Ile Leu Cys Asp Gly Gly

725 730 735

Leu Leu Met Gln Leu Asp Gly Asn Ser His Val Ile Tyr Ala Glu Glu

740 745 750

Glu Ala Ser Gly Thr Arg Leu Leu Ile Asp Gly Lys Thr Cys Met Leu

755 760 765

Gln Asn Asp His Asp Pro Ser Lys Leu Leu Ala Glu Thr Pro Cys Lys

770 775 780

Leu Leu Arg Phe Leu Val Ala Asp Gly Ala His Val Asp Ala Asp Val

785 790 795 800

Pro Tyr Ala Glu Val Glu Val Met Lys Met Cys Met Pro Leu Leu Ser

805 810 815

Pro Ala Ser Gly Val Ile His Val Val Met Ser Glu Gly Gln Ala Met

820 825 830

Gln Ala Gly Asp Leu Ile Ala Arg Leu Asp Leu Asp Asp Pro Ser Ala

835 840 845

Val Lys Arg Ala Glu Pro Phe Glu Asp Thr Phe Pro Gln Met Gly Leu

850 855 860

Pro Ile Ala Ala Ser Gly Gln Val His Lys Leu Cys Ala Ala Ser Leu

865 870 875 880

Asn Ala Cys Arg Met Ile Leu Ala Gly Tyr Glu His Asp Ile Asp Lys

885 890 895

Val Val Pro Glu Leu Val Tyr Cys Leu Asp Thr Pro Glu Leu Pro Phe

900 905 910

Leu Gln Trp Glu Glu Leu Met Ser Val Leu Ala Thr Arg Leu Pro Arg

915 920 925

Asn Leu Lys Ser Glu Leu Glu Gly Lys Tyr Glu Glu Tyr Lys Val Lys

930 935 940

Phe Asp Ser Gly Ile Ile Asn Asp Phe Pro Ala Asn Met Leu Arg Val

945 950 955 960

Ile Ile Glu Glu Asn Leu Ala Cys Gly Ser Glu Lys Glu Lys Ala Thr

965 970 975

Asn Glu Arg Leu Val Glu Pro Leu Met Ser Leu Leu Lys Ser Tyr Glu

980 985 990

Gly Gly Arg Glu Ser His Ala His Phe Val Val Lys Ser Leu Phe Glu

995 1000 1005

Glu Tyr Leu Tyr Val Glu Glu Leu Phe Ser Asp Gly Ile Gln Ser Asp

1010 1015 1020

Val Ile Glu Arg Leu Arg Leu Gln His Ser Lys Asp Leu Gln Lys Val

1025 1030 1035 1040

Val Asp Ile Val Leu Ser His Gln Ser Val Arg Asn Lys Thr Lys Leu

1045 1050 1055

Ile Leu Lys Leu Met Glu Ser Leu Val Tyr Pro Asn Pro Ala Ala Tyr

1060 1065 1070

Arg Asp Gln Leu Ile Arg Phe Ser Ser Leu Asn His Lys Ala Tyr Tyr

1075 1080 1085

Lys Leu Ala Leu Lys Ala Ser Glu Leu Leu Glu Gln Thr Lys Leu Ser

1090 1095 1100

Glu Leu Arg Ala Arg Ile Ala Arg Ser Leu Ser Glu Leu Glu Met Phe

1105 1110 1115 1120

Thr Glu Glu Ser Lys Gly Leu Ser Met His Lys Arg Glu Ile Ala Ile

1125 1130 1135

Lys Glu Ser Met Glu Asp Leu Val Thr Ala Pro Leu Pro Val Glu Asp

1140 1145 1150

Ala Leu Ile Ser Leu Phe Asp Cys Ser Asp Thr Thr Val Gln Gln Arg

1155 1160 1165

Val Ile Glu Thr Tyr Ile Ala Arg Leu Tyr Gln Pro His Leu Val Lys

1170 1175 1180

Asp Ser Ile Lys Met Lys Trp Ile Glu Ser Gly Val Ile Ala Leu Trp

1185 1190 1195 1200

Glu Phe Pro Glu Gly His Phe Asp Ala Arg Asn Gly Gly Ala Val Leu

1205 1210 1215

Gly Asp Lys Arg Trp Gly Ala Met Val Ile Val Lys Ser Leu Glu Ser

1220 1225 1230

Leu Ser Met Ala Ile Arg Phe Ala Leu Lys Glu Thr Ser His Tyr Thr

1235 1240 1245

Ser Ser Glu Gly Asn Met Met His Ile Ala Leu Leu Gly Ala Asp Asn

1250 1255 1260

Lys Met His Ile Ile Gln Glu Ser Gly Asp Asp Ala Asp Arg Ile Ala

1265 1270 1275 1280

Lys Leu Pro Leu Ile Leu Lys Asp Asn Val Thr Asp Leu His Ala Ser

1285 1290 1295

Gly Val Lys Thr Ile Ser Phe Ile Val Gln Arg Asp Glu Ala Arg Met

1300 1305 1310

Thr Met Arg Arg Thr Phe Leu Trp Ser Asp Glu Lys Leu Ser Tyr Glu

1315 1320 1325

Glu Glu Pro Ile Leu Arg His Val Glu Pro Pro Leu Ser Ala Leu Leu

1330 1335 1340

Glu Leu Asp Lys Leu Lys Val Lys Gly Tyr Asn Glu Met Lys Tyr Thr

1345 1350 1355 1360

Pro Ser Arg Asp Arg Gln Trp His Ile Tyr Thr Leu Arg Asn Thr Glu

1365 1370 1375

Asn Pro Lys Met Leu His Arg Val Phe Phe Arg Thr Leu Val Arg Gln

1380 1385 1390

Pro Ser Val Ser Asn Lys Phe Ser Ser Gly Gln Ile Gly Asp Met Glu

1395 1400 1405

Val Gly Ser Ala Glu Glu Pro Leu Ser Phe Thr Ser Thr Ser Ile Leu

1410 1415 1420

Arg Ser Leu Met Thr Ala Ile Glu Glu Leu Glu Leu His Ala Ile Arg

1425 1430 1435 1440

Thr Gly His Ser His Met Tyr Leu His Val Leu Lys Glu Gln Lys Leu

1445 1450 1455

Leu Asp Leu Val Pro Val Ser Gly Asn Thr Val Leu Asp Val Gly Gln

1460 1465 1470

Asp Glu Ala Thr Ala Tyr Ser Leu Leu Lys Glu Met Ala Met Lys Ile

1475 1480 1485

His Glu Leu Val Gly Ala Arg Met His His Leu Ser Val Cys Gln Trp

1490 1495 1500

Glu Val Lys Leu Lys Leu Asp Cys Asp Gly Pro Ala Ser Gly Thr Trp

1505 1510 1515 1520

Arg Ile Val Thr Thr Asn Val Thr Ser His Thr Cys Thr Val Asp Ile

1525 1530 1535

Tyr Arg Glu Met Glu Asp Lys Glu Ser Arg Lys Leu Val Tyr His Pro

1540 1545 1550

Ala Thr Pro Ala Ala Gly Pro Leu His Gly Val Ala Leu Asn Asn Pro

1555 1560 1565

Tyr Gln Pro Leu Ser Val Ile Asp Leu Lys Arg Cys Ser Ala Arg Asn

1570 1575 1580

Asn Arg Thr Thr Tyr Cys Tyr Asp Phe Pro Leu Ala Phe Glu Thr Ala

1585 1590 1595 1600

Val Arg Lys Ser Trp Ser Ser Ser Thr Ser Gly Ala Ser Lys Gly Val

1605 1610 1615

Glu Asn Ala Gln Cys Tyr Val Lys Ala Thr Glu Leu Val Phe Ala Asp

1620 1625 1630

Lys His Gly Ser Trp Gly Thr Pro Leu Val Gln Met Asp Arg Pro Ala

1635 1640 1645

Gly Leu Asn Asp Ile Gly Met Val Ala Trp Thr Leu Lys Met Ser Thr

1650 1655 1660

Pro Glu Phe Pro Ser Gly Arg Glu Ile Ile Val Val Ala Asn Asp Ile

1665 1670 1675 1680

Thr Phe Arg Ala Gly Ser Phe Gly Pro Arg Glu Asp Ala Phe Phe Glu

1685 1690 1695

Ala Val Thr Asn Leu Ala Cys Glu Lys Lys Leu Pro Leu Ile Tyr Leu

1700 1705 1710

Ala Ala Asn Ser Gly Ala Arg Ile Gly Ile Ala Asp Glu Val Lys Ser

1715 1720 1725

Cys Phe Arg Val Gly Trp Ser Asp Asp Gly Ser Pro Glu Arg Gly Phe

1730 1735 1740

Gln Tyr Ile Tyr Leu Ser Glu Glu Asp Tyr Ala Arg Ile Gly Thr Ser

1745 1750 1755 1760

Val Ile Ala His Lys Met Gln Leu Asp Ser Gly Glu Ile Arg Trp Val

1765 1770 1775

Ile Asp Ser Val Val Gly Lys Glu Asp Gly Leu Gly Val Glu Asn Ile

1780 1785 1790

His Gly Ser Ala Ala Ile Ala Ser Ala Tyr Ser Arg Ala Tyr Lys Glu

1795 1800 1805

Thr Phe Thr Leu Thr Phe Val Thr Gly Arg Thr Val Gly Ile Gly Ala

1810 1815 1820

Tyr Leu Ala Arg Leu Gly Ile Arg Cys Ile Gln Arg Leu Asp Gln Pro

1825 1830 1835 1840

Ile Ile Leu Thr Gly Tyr Ser Ala Leu Asn Lys Leu Leu Gly Arg Glu

1845 1850 1855

Val Tyr Ser Ser His Met Gln Leu Gly Gly Pro Lys Ile Met Ala Thr

1860 1865 1870

Asn Gly Val Val His Leu Thr Val Ser Asp Asp Leu Glu Gly Val Ser

1875 1880 1885

Asn Ile Leu Arg Trp Leu Ser Tyr Val Pro Ala Tyr Ile Gly Gly Pro

1890 1895 1900

Leu Pro Val Thr Thr Pro Leu Asp Pro Pro Asp Arg Pro Val Ala Tyr

1905 1910 1915 1920

Ile Pro Glu Asn Ser Cys Asp Pro Arg Ala Ala Ile Arg Gly Val Asp

1925 1930 1935

Asp Ser Gln Gly Lys Trp Leu Gly Gly Met Phe Asp Lys Asp Ser Phe

1940 1945 1950

Val Glu Thr Phe Glu Gly Trp Ala Lys Thr Val Val Thr Gly Arg Ala

1955 1960 1965

Lys Leu Gly Gly Ile Pro Val Gly Val Ile Ala Val Glu Thr Gln Thr

1970 1975 1980

Met Met Gln Thr Ile Pro Ala Asp Pro Gly Gln Leu Asp Ser Arg Glu

1985 1990 1995 2000

Gln Ser Val Pro Arg Ala Gly Gln Val Trp Phe Pro Asp Ser Ala Thr

2005 2010 2015

Lys Thr Ala Gln Ala Leu Leu Asp Phe Asn Arg Glu Gly Leu Pro Leu

2020 2025 2030

Phe Ile Leu Ala Asn Trp Arg Gly Phe Ser Gly Gly Gln Arg Asp Leu

2035 2040 2045

Phe Glu Gly Ile Leu Gln Ala Gly Ser Thr Ile Val Glu Asn Leu Arg

2050 2055 2060

Thr Tyr Asn Gln Pro Val Phe Val Tyr Ile Pro Met Ala Ala Glu Leu

2065 2070 2075 2080

Arg Gly Gly Ala Trp Val Val Val Asp Ser Lys Ile Asn Pro Asp Arg

2085 2090 2095

Ile Glu Cys Tyr Ala Glu Arg Thr Ala Lys Gly Asn Val Leu Glu Pro

2100 2105 2110

Gln Gly Leu Ile Glu Ile Lys Phe Arg Ser Glu Glu Leu Gln Asp Cys

2115 2120 2125

Met Ser Arg Leu Asp Pro Thr Leu Ile Asp Leu Lys Ala Lys Leu Glu

2130 2135 2140

Val Ala Asn Lys Asn Gly Ser Ala Asp Thr Lys Ser Leu Gln Glu Asn

2145 2150 2155 2160

Ile Glu Ala Arg Thr Lys Gln Leu Met Pro Leu Tyr Thr Gln Ile Ala

2165 2170 2175

Ile Arg Phe Ala Glu Leu His Asp Thr Ser Leu Arg Met Ala Ala Lys

2180 2185 2190

Gly Val Ile Lys Lys Val Val Asp Trp Glu Glu Ser Arg Ser Phe Phe

2195 2200 2205

Tyr Lys Arg Leu Arg Arg Arg Ile Ser Glu Asp Val Leu Ala Lys Glu

2210 2215 2220

Ile Arg Ala Val Ala Gly Glu Gln Phe Ser His Gln Pro Ala Ile Glu

2225 2230 2235 2240

Leu Ile Lys Lys Trp Tyr Ser Ala Ser His Ala Ala Glu Trp Asp Asp

2245 2250 2255

Asp Asp Ala Phe Val Ala Trp Met Asp Asn Pro Glu Asn Tyr Lys Asp

2260 2265 2270

Tyr Ile Gln Tyr Leu Lys Ala Gln Arg Val Ser Gln Ser Leu Ser Ser

2275 2280 2285

Leu Ser Asp Ser Ser Ser Asp Leu Gln Ala Leu Pro Gln Gly Leu Ser

2290 2295 2300

Met Leu Leu Asp Lys Met Asp Pro Ser Arg Arg Ala Gln Leu Val Glu

2305 2310 2315 2320

Glu Ile Arg Lys Val Leu Gly

2325

<210> 19

<211> 6984

<212> DNA

<213> 水稻(Oryza sativa嘉花1号ACCase-6316突变核苷酸序列)

<400> 19

atgacatcca cacatgtggc gacattggga gttggtgccc aggcacctcc tcgtcaccag 60

aaaaagtcag ctggcactgc atttgtatca tctgggtcat caagaccctc ataccgaaag 120

aatggtcagc gtactcggtc acttagggaa gaaagcaatg gaggagtgtc tgattccaaa 180

aagcttaacc actctattcg ccaaggtctt gctggcatca ttgacctccc aaatgacgca 240

gcttcagaag ttgatatttc acatggttcc gaagatccca gggggcctac ggtcccaggt 300

tcctaccaaa tgaatgggat tatcaatgaa acacataatg ggaggcatgc ttcagtctcc 360

aaggttgttg agttttgtac ggcacttggt ggcaaaacac caattcacag tgtattagtg 420

gccaacaatg gaatggcagc agctaagttc atgcggagtg tccgaacatg ggctaatgat 480

acttttggat cagagaaggc aattcagctg atagctatgg caactccgga ggatctgagg 540

ataaatgcag agcacatcag aattgccgat caatttgtag aggtacctgg tggaacaaac 600

aacaacaact atgcaaatgt ccaactcata gtggagatag cagagagaac aggtgtttct 660

gctgtttggc ctggttgggg tcatgcatct gagaatcctg aacttccaga tgcgctgact 720

gcaaaaggaa ttgtttttct tgggccacca gcatcatcaa tgcatgcatt aggagacaag 780

gttggctcag ctctcattgc tcaagcagct ggagttccaa cacttgcttg gagtggatca 840

catgtggaag ttcctctgga gtgttgcttg gactcaatac ctgatgagat gtatagaaaa 900

gcttgtgtta ctaccacaga ggaagcagtt gcaagttgtc aggtggttgg ttatcctgcc 960

atgattaagg catcttgggg tggtggtggt aaaggaataa ggaaggttca taatgatgat 1020

gaggttagga cattatttaa gcaagttcaa ggcgaagtac ctggttcccc aatatttatc 1080

atgaggctag ctgctcagag tcgacatctt gaagttcagt tgctttgtga tcaatatggc 1140

aacgtagcag cacttcacag tcgagattgc agtgtacaac ggcgacacca aaagataatc 1200

gaggaaggac cagttactgt tgctcctcgt gagactgtga aagagcttga gcaggcagca 1260

cggaggcttg ctaaagctgt gggttatgtt ggtgctgcta ctgttgaata cctttacagc 1320

atggaaactg gtgaatatta ttttctggaa cttaatccac ggctacaggt tgagcatcct 1380

gtcactgagt ggatagctga agtaaatttg cctgcggctc aagttgctgt tggaatgggt 1440

ataccccttt ggcagattcc agagatcagg cgcttctacg gaatgaacca tggaggaggc 1500

tatgaccttt ggaggaaaac agcagctcta gcgactccat ttaactttga tgaagtagat 1560

tctaaatggc caaaaggcca ctgcgtagct gttagaataa ctagcgagga tccagatgat 1620

gggtttaagc ctactggtgg aaaagtaaag gagataagtt tcaagagtaa accaaatgtt 1680

tgggcctatt tctcagtaaa gtctggtgga ggcatccatg aattcgctga ttctcagttc 1740

ggacatgttt ttgcgtatgg aactactaga tcggcagcaa taactaccat ggctcttgca 1800

ctaaaagagg ttcaaattcg tggagaaatt cattcaaacg tagactacac agttgaccta 1860

ttaaatgcct cagattttag agaaaataag attcatactg gttggctgga taccaggata 1920

gccatgcgtg ttcaagctga gaggcctcca tggtatattt cagtcgttgg aggggcttta 1980

tataaaacag taactgccaa cacggccact gtttctgatt atgttggtta tcttaccaag 2040

ggccagattc caccaaagca tatatccctt gtctatacga ctgttgcttt gaatatagat 2100

gggaaaaaat atacaatcga tactgtgagg agtggacatg gtagctacag attgcgaatg 2160

aatggatcaa cggttgacgc aaatgtacaa atattatgtg atggtgggct tttaatgcag 2220

ctggatggaa acagccatgt aatttatgct gaagaagagg ccagtggtac acgacttctt 2280

attgatggaa agacatgcat gttacagaat gaccatgacc catcaaagtt attagctgag 2340

acaccatgca aacttcttcg tttcttggtt gctgatggtg ctcatgttga tgctgatgta 2400

ccatatgcgg aagttgaggt tatgaagatg tgcatgcccc tcttatcacc cgcttctggt 2460

gtcatacatg ttgtaatgtc tgagggccaa gcaatgcagg ctggtgatct tatagctagg 2520

ctggatcttg atgacccttc tgctgttaag agagctgagc cgttcgaaga tacttttcca 2580

caaatgggtc tccctattgc tgcttctggc caagttcaca aattatgtgc tgcaagtctg 2640

aatgcttgtc gaatgatcct tgcggggtat gagcatgata ttgacaaggt tgtgccagag 2700

ttggtatact gcctagacac tccggagctt cctttcctgc agtgggagga gcttatgtct 2760

gttttagcaa ctagacttcc aagaaatctt aaaagtgagt tggagggcaa atatgaggaa 2820

tacaaagtaa aatttgactc tgggataatc aatgatttcc ctgccaatat gctacgagtg 2880

ataattgagg aaaatcttgc atgtggttct gagaaggaga aggctacaaa tgagaggctt 2940

gttgagcctc ttatgagcct actgaagtca tatgagggtg ggagagaaag tcatgctcac 3000

tttgttgtca agtccctttt tgaggagtat ctctatgttg aagaattgtt cagtgatgga 3060

attcagtctg atgtgattga gcgtctgcgc cttcaacata gtaaagacct acagaaggtc 3120

gtagacattg tgttgtccca ccagagtgtt agaaataaaa ctaagctgat actaaaactc 3180

atggagagtc tggtctatcc aaatcctgct gcctacaggg atcaattgat tcgcttttct 3240

tcccttaatc acaaagcgta ttacaagttg gcacttaaag ctagtgaact tcttgaacaa 3300

acaaaactta gtgagctccg tgcaagaata gcaaggagcc tttcagagct ggagatgttt 3360

actgaggaaa gcaagggtct ctccatgcat aagcgagaaa ttgccattaa ggagagcatg 3420

gaagatttag tcactgctcc actgccagtt gaagatgcgc tcatttcttt atttgattgt 3480

agtgatacaa ctgttcaaca gagagtgatt gagacttata tagctcgatt ataccagcct 3540

catcttgtaa aggacagtat caaaatgaaa tggatagaat cgggtgttat tgctttatgg 3600

gaatttcctg aagggcattt tgatgcaaga aatggaggag cggttcttgg tgacaaaaga 3660

tggggtgcca tggtcattgt caagtctctt gaatcacttt caatggccat tagatttgca 3720

ctaaaggaga catcacacta cactagctct gagggcaata tgatgcatat tgctttgttg 3780

ggtgctgata ataagatgca tataattcaa gaaagtggtg atgatgctga cagaatagcc 3840

aaacttccct tgatactaaa ggataatgta accgatctgc atgcctctgg tgtgaaaaca 3900

ataagtttca ttgttcaaag agatgaagca cggatgacaa tgcgtcgtac cttcctttgg 3960

tctgatgaaa agctttctta tgaggaagag ccaattctcc ggcatgtgga acctcctctt 4020

tctgcacttc ttgagttgga caagttgaaa gtgaaaggat acaatgaaat gaagtatacc 4080

ccatcacggg atcgtcaatg gcatatctac acacttagaa atactgaaaa ccccaaaatg 4140

ttgcaccggg tatttttccg aacccttgtc aggcaaccca gtgtatccaa caagttttct 4200

tcgggccaga ttggtgacat ggaagttggg agtgctgaag aacctctgtc atttacatca 4260

accagcatat taagatcttt gatgactgct atagaggaat tggagcttca cgcaattaga 4320

actggccatt cacacatgta tttgcatgta ttgaaagaac aaaagcttct tgatcttgtt 4380

ccagtttcag ggaatacagt tttggatgtt ggtcaagatg aagctactgc atattcactt 4440

ttaaaagaaa tggctatgaa gatacatgaa cttgttggtg caagaatgca ccatctttct 4500

gtatgccaat gggaagtgaa acttaagttg gactgcgatg gtcctgccag tggtacctgg 4560

aggattgtaa caaccaatgt tactagtcac acttgcactg tggatatcta ccgtgagatg 4620

gaagataaag aatcacggaa gttagtatac catcccgcca ctccggcggc tggtcctctg 4680

catggtgtgg cactgaataa tccatatcag cctttgagtg tcattgatct caaacgctgt 4740

tctgctagga ataatagaac tacatactgc tatgattttc cactggcatt tgaaactgca 4800

gtgaggaagt catggtcctc tagtacctct ggtgcttcta aaggtgttga aaatgcccaa 4860

tgttatgtta aagctacaga gttggtattt gcggacaaac atgggtcatg gggcactcct 4920

ttagttcaaa tggaccggcc tgctgggctc aatgacattg gtatggtagc ttggaccttg 4980

aagatgtcca ctcctgaatt tcctagtggt agggagatta ttgttgttgc aaatgatatt 5040

acgttcagag ctggatcatt tggcccaagg gaagatgcat tttttgaagc tgttaccaac 5100

ctagcctgtg agaagaaact tcctcttatt tatttggcag caaattctgg tgctcgaatt 5160

ggcatagcag atgaagtgaa atcttgcttc cgtgttgggt ggtctgatga tggcagccct 5220

gaacgtgggt ttcagtacat ttatctaagc gaagaagact atgctcgtat tggcacttct 5280

gtcatagcac ataagatgca gctagacagt ggtgaaatta ggtgggttat tgattctgtt 5340

gtgggcaagg aagatggact tggtgtggag aatatacatg gaagtgctgc tattgccagt 5400

gcttattcta gggcatataa ggagacattt acacttacat ttgtgactgg aagaactgtt 5460

ggaataggag cttatcttgc tcgacttggc atccggtgca tacagcgtct tgaccagcct 5520

attattctta caggctattc tgcactgaac aagcttcttg ggcgggaagt gtacagctcc 5580

cacatgcagt tgggtggtcc caaaatcatg gcaactaatg gtgttgtcca tcttactgtt 5640

tcagatgacc ttgaaggcgt ttctaatata ttgaggtggc tcagttatgt tcctgcctac 5700

attggtggac cacttccagt aacaacaccg ttggacccac cggacagacc tgttgcatac 5760

attcctgaga actcgtgtga tcctcgagcg gctatccgtg gtgttgatga cagccaaggg 5820

aaatggttag gtggtatgtt tgataaagac agctttgtgg aaacatttga aggttgggct 5880

aagacagtgg ttactggcag agcaaagctt ggtggaattc cagtgggtgt gatagctgtg 5940

gagactcaga ccatgatgca aactatccct gctgaccctg gtcagcttga ttcccgtgag 6000

caatctgttc ctcgtgctgg acaagtgtgg tttccagatt ctgcaaccaa gactgcgcag 6060

gcattgctgg acttcaaccg tgaaggatta cctctgttca tcctcgctaa ctggagaggc 6120

ttctctggtg gacaaagaga tctttttgaa ggaattcttc aggctggctc gactattgtt 6180

gagaacctta ggacatacaa tcagcctgcc tttgtctaca ttcccatggc tgcagagcta 6240

cgaggagggg cttgggttgt ggttgatagc aagataaacc cagaccgcat tgagtgctat 6300

gctgagagga ctgcagaagg caatgttctg gaaccgcaag ggttaattga gatcaagttc 6360

aggtcagagg aactccagga ttgcatgagt cggcttgacc caacattaat tgatctgaaa 6420

gcaaaactcg aagtagcaaa taaaaatgga agtgctgaca caaaatcgct tcaagaaaat 6480

atagaagctc gaacaaaaca gttgatgcct ctatatactc agattgcgat acggtttgct 6540

gaattgcatg atacatccct cagaatggct gcgaaaggtg tgattaagaa agttgtggac 6600

tgggaagaat cacgatcttt cttctataag agattacgga ggaggatctc tgaggatgtt 6660

cttgcaaaag aaattagagc tgtagcaggt gagcagtttt cccaccaacc agcaatcgag 6720

ctgatcaaga aatggtattc agcttcacat gcagctgaat gggatgatga cgatgctttt 6780

gttgcttgga tggataaccc tgaaaactac aaggattata ttcaatatct taaggctcaa 6840

agagtatccc aatccctctc aagtctttca gattccagct cagatttgca agccctgcca 6900

cagggtcttt ccatgttact agataagatg gatccctcta gaagagctca acttgttgaa 6960

gaaatcagga aggtccttgg ttga 6984

<210> 20

<211> 2327

<212> PRT

<213> 水稻(Oryza sativa嘉花1号ACCase-6316突变氨基酸序列)

<400> 20

Met Thr Ser Thr His Val Ala Thr Leu Gly Val Gly Ala Gln Ala Pro

1 5 10 15

Pro Arg His Gln Lys Lys Ser Ala Gly Thr Ala Phe Val Ser Ser Gly

20 25 30

Ser Ser Arg Pro Ser Tyr Arg Lys Asn Gly Gln Arg Thr Arg Ser Leu

35 40 45

Arg Glu Glu Ser Asn Gly Gly Val Ser Asp Ser Lys Lys Leu Asn His

50 55 60

Ser Ile Arg Gln Gly Leu Ala Gly Ile Ile Asp Leu Pro Asn Asp Ala

65 70 75 80

Ala Ser Glu Val Asp Ile Ser His Gly Ser Glu Asp Pro Arg Gly Pro

85 90 95

Thr Val Pro Gly Ser Tyr Gln Met Asn Gly Ile Ile Asn Glu Thr His

100 105 110

Asn Gly Arg His Ala Ser Val Ser Lys Val Val Glu Phe Cys Thr Ala

115 120 125

Leu Gly Gly Lys Thr Pro Ile His Ser Val Leu Val Ala Asn Asn Gly

130 135 140

Met Ala Ala Ala Lys Phe Met Arg Ser Val Arg Thr Trp Ala Asn Asp

145 150 155 160

Thr Phe Gly Ser Glu Lys Ala Ile Gln Leu Ile Ala Met Ala Thr Pro

165 170 175

Glu Asp Leu Arg Ile Asn Ala Glu His Ile Arg Ile Ala Asp Gln Phe

180 185 190

Val Glu Val Pro Gly Gly Thr Asn Asn Asn Asn Tyr Ala Asn Val Gln

195 200 205

Leu Ile Val Glu Ile Ala Glu Arg Thr Gly Val Ser Ala Val Trp Pro

210 215 220

Gly Trp Gly His Ala Ser Glu Asn Pro Glu Leu Pro Asp Ala Leu Thr

225 230 235 240

Ala Lys Gly Ile Val Phe Leu Gly Pro Pro Ala Ser Ser Met His Ala

245 250 255

Leu Gly Asp Lys Val Gly Ser Ala Leu Ile Ala Gln Ala Ala Gly Val

260 265 270

Pro Thr Leu Ala Trp Ser Gly Ser His Val Glu Val Pro Leu Glu Cys

275 280 285

Cys Leu Asp Ser Ile Pro Asp Glu Met Tyr Arg Lys Ala Cys Val Thr

290 295 300

Thr Thr Glu Glu Ala Val Ala Ser Cys Gln Val Val Gly Tyr Pro Ala

305 310 315 320

Met Ile Lys Ala Ser Trp Gly Gly Gly Gly Lys Gly Ile Arg Lys Val

325 330 335

His Asn Asp Asp Glu Val Arg Thr Leu Phe Lys Gln Val Gln Gly Glu

340 345 350

Val Pro Gly Ser Pro Ile Phe Ile Met Arg Leu Ala Ala Gln Ser Arg

355 360 365

His Leu Glu Val Gln Leu Leu Cys Asp Gln Tyr Gly Asn Val Ala Ala

370 375 380

Leu His Ser Arg Asp Cys Ser Val Gln Arg Arg His Gln Lys Ile Ile

385 390 395 400

Glu Glu Gly Pro Val Thr Val Ala Pro Arg Glu Thr Val Lys Glu Leu

405 410 415

Glu Gln Ala Ala Arg Arg Leu Ala Lys Ala Val Gly Tyr Val Gly Ala

420 425 430

Ala Thr Val Glu Tyr Leu Tyr Ser Met Glu Thr Gly Glu Tyr Tyr Phe

435 440 445

Leu Glu Leu Asn Pro Arg Leu Gln Val Glu His Pro Val Thr Glu Trp

450 455 460

Ile Ala Glu Val Asn Leu Pro Ala Ala Gln Val Ala Val Gly Met Gly

465 470 475 480

Ile Pro Leu Trp Gln Ile Pro Glu Ile Arg Arg Phe Tyr Gly Met Asn

485 490 495

His Gly Gly Gly Tyr Asp Leu Trp Arg Lys Thr Ala Ala Leu Ala Thr

500 505 510

Pro Phe Asn Phe Asp Glu Val Asp Ser Lys Trp Pro Lys Gly His Cys

515 520 525

Val Ala Val Arg Ile Thr Ser Glu Asp Pro Asp Asp Gly Phe Lys Pro

530 535 540

Thr Gly Gly Lys Val Lys Glu Ile Ser Phe Lys Ser Lys Pro Asn Val

545 550 555 560

Trp Ala Tyr Phe Ser Val Lys Ser Gly Gly Gly Ile His Glu Phe Ala

565 570 575

Asp Ser Gln Phe Gly His Val Phe Ala Tyr Gly Thr Thr Arg Ser Ala

580 585 590

Ala Ile Thr Thr Met Ala Leu Ala Leu Lys Glu Val Gln Ile Arg Gly

595 600 605

Glu Ile His Ser Asn Val Asp Tyr Thr Val Asp Leu Leu Asn Ala Ser

610 615 620

Asp Phe Arg Glu Asn Lys Ile His Thr Gly Trp Leu Asp Thr Arg Ile

625 630 635 640

Ala Met Arg Val Gln Ala Glu Arg Pro Pro Trp Tyr Ile Ser Val Val

645 650 655

Gly Gly Ala Leu Tyr Lys Thr Val Thr Ala Asn Thr Ala Thr Val Ser

660 665 670

Asp Tyr Val Gly Tyr Leu Thr Lys Gly Gln Ile Pro Pro Lys His Ile

675 680 685

Ser Leu Val Tyr Thr Thr Val Ala Leu Asn Ile Asp Gly Lys Lys Tyr

690 695 700

Thr Ile Asp Thr Val Arg Ser Gly His Gly Ser Tyr Arg Leu Arg Met

705 710 715 720

Asn Gly Ser Thr Val Asp Ala Asn Val Gln Ile Leu Cys Asp Gly Gly

725 730 735

Leu Leu Met Gln Leu Asp Gly Asn Ser His Val Ile Tyr Ala Glu Glu

740 745 750

Glu Ala Ser Gly Thr Arg Leu Leu Ile Asp Gly Lys Thr Cys Met Leu

755 760 765

Gln Asn Asp His Asp Pro Ser Lys Leu Leu Ala Glu Thr Pro Cys Lys

770 775 780

Leu Leu Arg Phe Leu Val Ala Asp Gly Ala His Val Asp Ala Asp Val

785 790 795 800

Pro Tyr Ala Glu Val Glu Val Met Lys Met Cys Met Pro Leu Leu Ser

805 810 815

Pro Ala Ser Gly Val Ile His Val Val Met Ser Glu Gly Gln Ala Met

820 825 830

Gln Ala Gly Asp Leu Ile Ala Arg Leu Asp Leu Asp Asp Pro Ser Ala

835 840 845

Val Lys Arg Ala Glu Pro Phe Glu Asp Thr Phe Pro Gln Met Gly Leu

850 855 860

Pro Ile Ala Ala Ser Gly Gln Val His Lys Leu Cys Ala Ala Ser Leu

865 870 875 880

Asn Ala Cys Arg Met Ile Leu Ala Gly Tyr Glu His Asp Ile Asp Lys

885 890 895

Val Val Pro Glu Leu Val Tyr Cys Leu Asp Thr Pro Glu Leu Pro Phe

900 905 910

Leu Gln Trp Glu Glu Leu Met Ser Val Leu Ala Thr Arg Leu Pro Arg

915 920 925

Asn Leu Lys Ser Glu Leu Glu Gly Lys Tyr Glu Glu Tyr Lys Val Lys

930 935 940

Phe Asp Ser Gly Ile Ile Asn Asp Phe Pro Ala Asn Met Leu Arg Val

945 950 955 960

Ile Ile Glu Glu Asn Leu Ala Cys Gly Ser Glu Lys Glu Lys Ala Thr

965 970 975

Asn Glu Arg Leu Val Glu Pro Leu Met Ser Leu Leu Lys Ser Tyr Glu

980 985 990

Gly Gly Arg Glu Ser His Ala His Phe Val Val Lys Ser Leu Phe Glu

995 1000 1005

Glu Tyr Leu Tyr Val Glu Glu Leu Phe Ser Asp Gly Ile Gln Ser Asp

1010 1015 1020

Val Ile Glu Arg Leu Arg Leu Gln His Ser Lys Asp Leu Gln Lys Val

1025 1030 1035 1040

Val Asp Ile Val Leu Ser His Gln Ser Val Arg Asn Lys Thr Lys Leu

1045 1050 1055

Ile Leu Lys Leu Met Glu Ser Leu Val Tyr Pro Asn Pro Ala Ala Tyr

1060 1065 1070

Arg Asp Gln Leu Ile Arg Phe Ser Ser Leu Asn His Lys Ala Tyr Tyr

1075 1080 1085

Lys Leu Ala Leu Lys Ala Ser Glu Leu Leu Glu Gln Thr Lys Leu Ser

1090 1095 1100

Glu Leu Arg Ala Arg Ile Ala Arg Ser Leu Ser Glu Leu Glu Met Phe

1105 1110 1115 1120

Thr Glu Glu Ser Lys Gly Leu Ser Met His Lys Arg Glu Ile Ala Ile

1125 1130 1135

Lys Glu Ser Met Glu Asp Leu Val Thr Ala Pro Leu Pro Val Glu Asp

1140 1145 1150

Ala Leu Ile Ser Leu Phe Asp Cys Ser Asp Thr Thr Val Gln Gln Arg

1155 1160 1165

Val Ile Glu Thr Tyr Ile Ala Arg Leu Tyr Gln Pro His Leu Val Lys

1170 1175 1180

Asp Ser Ile Lys Met Lys Trp Ile Glu Ser Gly Val Ile Ala Leu Trp

1185 1190 1195 1200

Glu Phe Pro Glu Gly His Phe Asp Ala Arg Asn Gly Gly Ala Val Leu

1205 1210 1215

Gly Asp Lys Arg Trp Gly Ala Met Val Ile Val Lys Ser Leu Glu Ser

1220 1225 1230

Leu Ser Met Ala Ile Arg Phe Ala Leu Lys Glu Thr Ser His Tyr Thr

1235 1240 1245

Ser Ser Glu Gly Asn Met Met His Ile Ala Leu Leu Gly Ala Asp Asn

1250 1255 1260

Lys Met His Ile Ile Gln Glu Ser Gly Asp Asp Ala Asp Arg Ile Ala

1265 1270 1275 1280

Lys Leu Pro Leu Ile Leu Lys Asp Asn Val Thr Asp Leu His Ala Ser

1285 1290 1295

Gly Val Lys Thr Ile Ser Phe Ile Val Gln Arg Asp Glu Ala Arg Met

1300 1305 1310

Thr Met Arg Arg Thr Phe Leu Trp Ser Asp Glu Lys Leu Ser Tyr Glu

1315 1320 1325

Glu Glu Pro Ile Leu Arg His Val Glu Pro Pro Leu Ser Ala Leu Leu

1330 1335 1340

Glu Leu Asp Lys Leu Lys Val Lys Gly Tyr Asn Glu Met Lys Tyr Thr

1345 1350 1355 1360

Pro Ser Arg Asp Arg Gln Trp His Ile Tyr Thr Leu Arg Asn Thr Glu

1365 1370 1375

Asn Pro Lys Met Leu His Arg Val Phe Phe Arg Thr Leu Val Arg Gln

1380 1385 1390

Pro Ser Val Ser Asn Lys Phe Ser Ser Gly Gln Ile Gly Asp Met Glu

1395 1400 1405

Val Gly Ser Ala Glu Glu Pro Leu Ser Phe Thr Ser Thr Ser Ile Leu

1410 1415 1420

Arg Ser Leu Met Thr Ala Ile Glu Glu Leu Glu Leu His Ala Ile Arg

1425 1430 1435 1440

Thr Gly His Ser His Met Tyr Leu His Val Leu Lys Glu Gln Lys Leu

1445 1450 1455

Leu Asp Leu Val Pro Val Ser Gly Asn Thr Val Leu Asp Val Gly Gln

1460 1465 1470

Asp Glu Ala Thr Ala Tyr Ser Leu Leu Lys Glu Met Ala Met Lys Ile

1475 1480 1485

His Glu Leu Val Gly Ala Arg Met His His Leu Ser Val Cys Gln Trp

1490 1495 1500

Glu Val Lys Leu Lys Leu Asp Cys Asp Gly Pro Ala Ser Gly Thr Trp

1505 1510 1515 1520

Arg Ile Val Thr Thr Asn Val Thr Ser His Thr Cys Thr Val Asp Ile

1525 1530 1535

Tyr Arg Glu Met Glu Asp Lys Glu Ser Arg Lys Leu Val Tyr His Pro

1540 1545 1550

Ala Thr Pro Ala Ala Gly Pro Leu His Gly Val Ala Leu Asn Asn Pro

1555 1560 1565

Tyr Gln Pro Leu Ser Val Ile Asp Leu Lys Arg Cys Ser Ala Arg Asn

1570 1575 1580

Asn Arg Thr Thr Tyr Cys Tyr Asp Phe Pro Leu Ala Phe Glu Thr Ala

1585 1590 1595 1600

Val Arg Lys Ser Trp Ser Ser Ser Thr Ser Gly Ala Ser Lys Gly Val

1605 1610 1615

Glu Asn Ala Gln Cys Tyr Val Lys Ala Thr Glu Leu Val Phe Ala Asp

1620 1625 1630

Lys His Gly Ser Trp Gly Thr Pro Leu Val Gln Met Asp Arg Pro Ala

1635 1640 1645

Gly Leu Asn Asp Ile Gly Met Val Ala Trp Thr Leu Lys Met Ser Thr

1650 1655 1660

Pro Glu Phe Pro Ser Gly Arg Glu Ile Ile Val Val Ala Asn Asp Ile

1665 1670 1675 1680

Thr Phe Arg Ala Gly Ser Phe Gly Pro Arg Glu Asp Ala Phe Phe Glu

1685 1690 1695

Ala Val Thr Asn Leu Ala Cys Glu Lys Lys Leu Pro Leu Ile Tyr Leu

1700 1705 1710

Ala Ala Asn Ser Gly Ala Arg Ile Gly Ile Ala Asp Glu Val Lys Ser

1715 1720 1725

Cys Phe Arg Val Gly Trp Ser Asp Asp Gly Ser Pro Glu Arg Gly Phe

1730 1735 1740

Gln Tyr Ile Tyr Leu Ser Glu Glu Asp Tyr Ala Arg Ile Gly Thr Ser

1745 1750 1755 1760

Val Ile Ala His Lys Met Gln Leu Asp Ser Gly Glu Ile Arg Trp Val

1765 1770 1775

Ile Asp Ser Val Val Gly Lys Glu Asp Gly Leu Gly Val Glu Asn Ile

1780 1785 1790

His Gly Ser Ala Ala Ile Ala Ser Ala Tyr Ser Arg Ala Tyr Lys Glu

1795 1800 1805

Thr Phe Thr Leu Thr Phe Val Thr Gly Arg Thr Val Gly Ile Gly Ala

1810 1815 1820

Tyr Leu Ala Arg Leu Gly Ile Arg Cys Ile Gln Arg Leu Asp Gln Pro

1825 1830 1835 1840

Ile Ile Leu Thr Gly Tyr Ser Ala Leu Asn Lys Leu Leu Gly Arg Glu

1845 1850 1855

Val Tyr Ser Ser His Met Gln Leu Gly Gly Pro Lys Ile Met Ala Thr

1860 1865 1870

Asn Gly Val Val His Leu Thr Val Ser Asp Asp Leu Glu Gly Val Ser

1875 1880 1885

Asn Ile Leu Arg Trp Leu Ser Tyr Val Pro Ala Tyr Ile Gly Gly Pro

1890 1895 1900

Leu Pro Val Thr Thr Pro Leu Asp Pro Pro Asp Arg Pro Val Ala Tyr

1905 1910 1915 1920

Ile Pro Glu Asn Ser Cys Asp Pro Arg Ala Ala Ile Arg Gly Val Asp

1925 1930 1935

Asp Ser Gln Gly Lys Trp Leu Gly Gly Met Phe Asp Lys Asp Ser Phe

1940 1945 1950

Val Glu Thr Phe Glu Gly Trp Ala Lys Thr Val Val Thr Gly Arg Ala

1955 1960 1965

Lys Leu Gly Gly Ile Pro Val Gly Val Ile Ala Val Glu Thr Gln Thr

1970 1975 1980

Met Met Gln Thr Ile Pro Ala Asp Pro Gly Gln Leu Asp Ser Arg Glu

1985 1990 1995 2000

Gln Ser Val Pro Arg Ala Gly Gln Val Trp Phe Pro Asp Ser Ala Thr

2005 2010 2015

Lys Thr Ala Gln Ala Leu Leu Asp Phe Asn Arg Glu Gly Leu Pro Leu

2020 2025 2030

Phe Ile Leu Ala Asn Trp Arg Gly Phe Ser Gly Gly Gln Arg Asp Leu

2035 2040 2045

Phe Glu Gly Ile Leu Gln Ala Gly Ser Thr Ile Val Glu Asn Leu Arg

2050 2055 2060

Thr Tyr Asn Gln Pro Ala Phe Val Tyr Ile Pro Met Ala Ala Glu Leu

2065 2070 2075 2080

Arg Gly Gly Ala Trp Val Val Val Asp Ser Lys Ile Asn Pro Asp Arg

2085 2090 2095

Ile Glu Cys Tyr Ala Glu Arg Thr Ala Glu Gly Asn Val Leu Glu Pro

2100 2105 2110

Gln Gly Leu Ile Glu Ile Lys Phe Arg Ser Glu Glu Leu Gln Asp Cys

2115 2120 2125

Met Ser Arg Leu Asp Pro Thr Leu Ile Asp Leu Lys Ala Lys Leu Glu

2130 2135 2140

Val Ala Asn Lys Asn Gly Ser Ala Asp Thr Lys Ser Leu Gln Glu Asn

2145 2150 2155 2160

Ile Glu Ala Arg Thr Lys Gln Leu Met Pro Leu Tyr Thr Gln Ile Ala

2165 2170 2175

Ile Arg Phe Ala Glu Leu His Asp Thr Ser Leu Arg Met Ala Ala Lys

2180 2185 2190

Gly Val Ile Lys Lys Val Val Asp Trp Glu Glu Ser Arg Ser Phe Phe

2195 2200 2205

Tyr Lys Arg Leu Arg Arg Arg Ile Ser Glu Asp Val Leu Ala Lys Glu

2210 2215 2220

Ile Arg Ala Val Ala Gly Glu Gln Phe Ser His Gln Pro Ala Ile Glu

2225 2230 2235 2240

Leu Ile Lys Lys Trp Tyr Ser Ala Ser His Ala Ala Glu Trp Asp Asp

2245 2250 2255

Asp Asp Ala Phe Val Ala Trp Met Asp Asn Pro Glu Asn Tyr Lys Asp

2260 2265 2270

Tyr Ile Gln Tyr Leu Lys Ala Gln Arg Val Ser Gln Ser Leu Ser Ser

2275 2280 2285

Leu Ser Asp Ser Ser Ser Asp Leu Gln Ala Leu Pro Gln Gly Leu Ser

2290 2295 2300

Met Leu Leu Asp Lys Met Asp Pro Ser Arg Arg Ala Gln Leu Val Glu

2305 2310 2315 2320

Glu Ile Arg Lys Val Leu Gly

2325

<210> 21

<211> 6984

<212> DNA

<213> 水稻(Oryza sativa嘉花1号ACCase野生型基因序列)

<400> 21

atgacatcca cacatgtggc gacattggga gttggtgccc aggcacctcc tcgtcaccag 60

aaaaagtcag ctggcactgc atttgtatca tctgggtcat caagaccctc ataccgaaag 120

aatggtcagc gtactcggtc acttagggaa gaaagcaatg gaggagtgtc tgattccaaa 180

aagcttaacc actctattcg ccaaggtctt gctggcatca ttgacctccc aaatgacgca 240

gcttcagaag ttgatatttc acatggttcc gaagatccca gggggcctac ggtcccaggt 300

tcctaccaaa tgaatgggat tatcaatgaa acacataatg ggaggcatgc ttcagtctcc 360

aaggttgttg agttttgtac ggcacttggt ggcaaaacac caattcacag tgtattagtg 420

gccaacaatg gaatggcagc agctaagttc atgcggagtg tccgaacatg ggctaatgat 480

acttttggat cagagaaggc aattcagctg atagctatgg caactccgga ggatctgagg 540

ataaatgcag agcacatcag aattgccgat caatttgtag aggtacctgg tggaacaaac 600

aacaacaact atgcaaatgt ccaactcata gtggagatag cagagagaac aggtgtttct 660

gctgtttggc ctggttgggg tcatgcatct gagaatcctg aacttccaga tgcgctgact 720

gcaaaaggaa ttgtttttct tgggccacca gcatcatcaa tgcatgcatt aggagacaag 780

gttggctcag ctctcattgc tcaagcagct ggagttccaa cacttgcttg gagtggatca 840

catgtggaag ttcctctgga gtgttgcttg gactcaatac ctgatgagat gtatagaaaa 900

gcttgtgtta ctaccacaga ggaagcagtt gcaagttgtc aggtggttgg ttatcctgcc 960

atgattaagg catcttgggg tggtggtggt aaaggaataa ggaaggttca taatgatgat 1020

gaggttagga cattatttaa gcaagttcaa ggcgaagtac ctggttcccc aatatttatc 1080

atgaggctag ctgctcagag tcgacatctt gaagttcagt tgctttgtga tcaatatggc 1140

aacgtagcag cacttcacag tcgagattgc agtgtacaac ggcgacacca aaagataatc 1200

gaggaaggac cagttactgt tgctcctcgt gagactgtga aagagcttga gcaggcagca 1260

cggaggcttg ctaaagctgt gggttatgtt ggtgctgcta ctgttgaata cctttacagc 1320

atggaaactg gtgaatatta ttttctggaa cttaatccac ggctacaggt tgagcatcct 1380

gtcactgagt ggatagctga agtaaatttg cctgcggctc aagttgctgt tggaatgggt 1440

ataccccttt ggcagattcc agagatcagg cgcttctacg gaatgaacca tggaggaggc 1500

tatgaccttt ggaggaaaac agcagctcta gcgactccat ttaactttga tgaagtagat 1560

tctaaatggc caaaaggcca ctgcgtagct gttagaataa ctagcgagga tccagatgat 1620

gggtttaagc ctactggtgg aaaagtaaag gagataagtt tcaagagtaa accaaatgtt 1680

tgggcctatt tctcagtaaa gtctggtgga ggcatccatg aattcgctga ttctcagttc 1740

ggacatgttt ttgcgtatgg aactactaga tcggcagcaa taactaccat ggctcttgca 1800

ctaaaagagg ttcaaattcg tggagaaatt cattcaaacg tagactacac agttgaccta 1860

ttaaatgcct cagattttag agaaaataag attcatactg gttggctgga taccaggata 1920

gccatgcgtg ttcaagctga gaggcctcca tggtatattt cagtcgttgg aggggcttta 1980

tataaaacag taactgccaa cacggccact gtttctgatt atgttggtta tcttaccaag 2040

ggccagattc caccaaagca tatatccctt gtctatacga ctgttgcttt gaatatagat 2100

gggaaaaaat atacaatcga tactgtgagg agtggacatg gtagctacag attgcgaatg 2160

aatggatcaa cggttgacgc aaatgtacaa atattatgtg atggtgggct tttaatgcag 2220

ctggatggaa acagccatgt aatttatgct gaagaagagg ccagtggtac acgacttctt 2280

attgatggaa agacatgcat gttacagaat gaccatgacc catcaaagtt attagctgag 2340

acaccatgca aacttcttcg tttcttggtt gctgatggtg ctcatgttga tgctgatgta 2400

ccatatgcgg aagttgaggt tatgaagatg tgcatgcccc tcttatcacc cgcttctggt 2460

gtcatacatg ttgtaatgtc tgagggccaa gcaatgcagg ctggtgatct tatagctagg 2520

ctggatcttg atgacccttc tgctgttaag agagctgagc cgttcgaaga tacttttcca 2580

caaatgggtc tccctattgc tgcttctggc caagttcaca aattatgtgc tgcaagtctg 2640

aatgcttgtc gaatgatcct tgcggggtat gagcatgata ttgacaaggt tgtgccagag 2700

ttggtatact gcctagacac tccggagctt cctttcctgc agtgggagga gcttatgtct 2760

gttttagcaa ctagacttcc aagaaatctt aaaagtgagt tggagggcaa atatgaggaa 2820

tacaaagtaa aatttgactc tgggataatc aatgatttcc ctgccaatat gctacgagtg 2880

ataattgagg aaaatcttgc atgtggttct gagaaggaga aggctacaaa tgagaggctt 2940

gttgagcctc ttatgagcct actgaagtca tatgagggtg ggagagaaag tcatgctcac 3000

tttgttgtca agtccctttt tgaggagtat ctctatgttg aagaattgtt cagtgatgga 3060

attcagtctg atgtgattga gcgtctgcgc cttcaacata gtaaagacct acagaaggtc 3120

gtagacattg tgttgtccca ccagagtgtt agaaataaaa ctaagctgat actaaaactc 3180

atggagagtc tggtctatcc aaatcctgct gcctacaggg atcaattgat tcgcttttct 3240

tcccttaatc acaaagcgta ttacaagttg gcacttaaag ctagtgaact tcttgaacaa 3300

acaaaactta gtgagctccg tgcaagaata gcaaggagcc tttcagagct ggagatgttt 3360

actgaggaaa gcaagggtct ctccatgcat aagcgagaaa ttgccattaa ggagagcatg 3420

gaagatttag tcactgctcc actgccagtt gaagatgcgc tcatttcttt atttgattgt 3480

agtgatacaa ctgttcaaca gagagtgatt gagacttata tagctcgatt ataccagcct 3540

catcttgtaa aggacagtat caaaatgaaa tggatagaat cgggtgttat tgctttatgg 3600

gaatttcctg aagggcattt tgatgcaaga aatggaggag cggttcttgg tgacaaaaga 3660

tggggtgcca tggtcattgt caagtctctt gaatcacttt caatggccat tagatttgca 3720

ctaaaggaga catcacacta cactagctct gagggcaata tgatgcatat tgctttgttg 3780

ggtgctgata ataagatgca tataattcaa gaaagtggtg atgatgctga cagaatagcc 3840

aaacttccct tgatactaaa ggataatgta accgatctgc atgcctctgg tgtgaaaaca 3900

ataagtttca ttgttcaaag agatgaagca cggatgacaa tgcgtcgtac cttcctttgg 3960

tctgatgaaa agctttctta tgaggaagag ccaattctcc ggcatgtgga acctcctctt 4020

tctgcacttc ttgagttgga caagttgaaa gtgaaaggat acaatgaaat gaagtatacc 4080

ccatcacggg atcgtcaatg gcatatctac acacttagaa atactgaaaa ccccaaaatg 4140

ttgcaccggg tatttttccg aacccttgtc aggcaaccca gtgtatccaa caagttttct 4200

tcgggccaga ttggtgacat ggaagttggg agtgctgaag aacctctgtc atttacatca 4260

accagcatat taagatcttt gatgactgct atagaggaat tggagcttca cgcaattaga 4320

actggccatt cacacatgta tttgcatgta ttgaaagaac aaaagcttct tgatcttgtt 4380

ccagtttcag ggaatacagt tttggatgtt ggtcaagatg aagctactgc atattcactt 4440

ttaaaagaaa tggctatgaa gatacatgaa cttgttggtg caagaatgca ccatctttct 4500

gtatgccaat gggaagtgaa acttaagttg gactgcgatg gtcctgccag tggtacctgg 4560

aggattgtaa caaccaatgt tactagtcac acttgcactg tggatatcta ccgtgagatg 4620

gaagataaag aatcacggaa gttagtatac catcccgcca ctccggcggc tggtcctctg 4680

catggtgtgg cactgaataa tccatatcag cctttgagtg tcattgatct caaacgctgt 4740

tctgctagga ataatagaac tacatactgc tatgattttc cactggcatt tgaaactgca 4800

gtgaggaagt catggtcctc tagtacctct ggtgcttcta aaggtgttga aaatgcccaa 4860

tgttatgtta aagctacaga gttggtattt gcggacaaac atgggtcatg gggcactcct 4920

ttagttcaaa tggaccggcc tgctgggctc aatgacattg gtatggtagc ttggaccttg 4980

aagatgtcca ctcctgaatt tcctagtggt agggagatta ttgttgttgc aaatgatatt 5040

acgttcagag ctggatcatt tggcccaagg gaagatgcat tttttgaagc tgttaccaac 5100

ctagcctgtg agaagaaact tcctcttatt tatttggcag caaattctgg tgctcgaatt 5160

ggcatagcag atgaagtgaa atcttgcttc cgtgttgggt ggtctgatga tggcagccct 5220

gaacgtgggt ttcagtacat ttatctaagc gaagaagact atgctcgtat tggcacttct 5280

gtcatagcac ataagatgca gctagacagt ggtgaaatta ggtgggttat tgattctgtt 5340

gtgggcaagg aagatggact tggtgtggag aatatacatg gaagtgctgc tattgccagt 5400

gcttattcta gggcatataa ggagacattt acacttacat ttgtgactgg aagaactgtt 5460

ggaataggag cttatcttgc tcgacttggc atccggtgca tacagcgtct tgaccagcct 5520

attattctta caggctattc tgcactgaac aagcttcttg ggcgggaagt gtacagctcc 5580

cacatgcagt tgggtggtcc caaaatcatg gcaactaatg gtgttgtcca tcttactgtt 5640

tcagatgacc ttgaaggcgt ttctaatata ttgaggtggc tcagttatgt tcctgcctac 5700

attggtggac cacttccagt aacaacaccg ttggacccac cggacagacc tgttgcatac 5760

attcctgaga actcgtgtga tcctcgagcg gctatccgtg gtgttgatga cagccaaggg 5820

aaatggttag gtggtatgtt tgataaagac agctttgtgg aaacatttga aggttgggct 5880

aagacagtgg ttactggcag agcaaagctt ggtggaattc cagtgggtgt gatagctgtg 5940

gagactcaga ccatgatgca aactatccct gctgaccctg gtcagcttga ttcccgtgag 6000

caatctgttc ctcgtgctgg acaagtgtgg tttccagatt ctgcaaccaa gactgcgcag 6060

gcattgctgg acttcaaccg tgaaggatta cctctgttca tcctcgctaa ctggagaggc 6120

ttctctggtg gacaaagaga tctttttgaa ggaattcttc aggctggctc gactattgtt 6180

gagaacctta ggacatacaa tcagcctgcc tttgtctaca ttcccatggc tgcagagcta 6240

cgaggagggg cttgggttgt ggttgatagc aagataaacc cagaccgcat tgagtgctat 6300

gctgagagga ctgcaaaagg caatgttctg gaaccgcaag ggttaattga gatcaagttc 6360

aggtcagagg aactccagga ttgcatgagt cggcttgacc caacattaat tgatctgaaa 6420

gcaaaactcg aagtagcaaa taaaaatgga agtgctgaca caaaatcgct tcaagaaaat 6480

atagaagctc gaacaaaaca gttgatgcct ctatatactc agattgcgat acggtttgct 6540

gaattgcatg atacatccct cagaatggct gcgaaaggtg tgattaagaa agttgtggac 6600

tgggaagaat cacgatcttt cttctataag agattacgga ggaggatctc tgaggatgtt 6660

cttgcaaaag aaattagagc tgtagcaggt gagcagtttt cccaccaacc agcaatcgag 6720

ctgatcaaga aatggtattc agcttcacat gcagctgaat gggatgatga cgatgctttt 6780

gttgcttgga tggataaccc tgaaaactac aaggattata ttcaatatct taaggctcaa 6840

agagtatccc aatccctctc aagtctttca gattccagct cagatttgca agccctgcca 6900

cagggtcttt ccatgttact agataagatg gatccctcta gaagagctca acttgttgaa 6960

gaaatcagga aggtccttgg ttga 6984

<210> 22

<211> 2327

<212> PRT

<213> 水稻(Oryza sativa嘉花1号ACCase野生型氨基酸序列)

<400> 22

Met Thr Ser Thr His Val Ala Thr Leu Gly Val Gly Ala Gln Ala Pro

1 5 10 15

Pro Arg His Gln Lys Lys Ser Ala Gly Thr Ala Phe Val Ser Ser Gly

20 25 30

Ser Ser Arg Pro Ser Tyr Arg Lys Asn Gly Gln Arg Thr Arg Ser Leu

35 40 45

Arg Glu Glu Ser Asn Gly Gly Val Ser Asp Ser Lys Lys Leu Asn His

50 55 60

Ser Ile Arg Gln Gly Leu Ala Gly Ile Ile Asp Leu Pro Asn Asp Ala

65 70 75 80

Ala Ser Glu Val Asp Ile Ser His Gly Ser Glu Asp Pro Arg Gly Pro

85 90 95

Thr Val Pro Gly Ser Tyr Gln Met Asn Gly Ile Ile Asn Glu Thr His

100 105 110

Asn Gly Arg His Ala Ser Val Ser Lys Val Val Glu Phe Cys Thr Ala

115 120 125

Leu Gly Gly Lys Thr Pro Ile His Ser Val Leu Val Ala Asn Asn Gly

130 135 140

Met Ala Ala Ala Lys Phe Met Arg Ser Val Arg Thr Trp Ala Asn Asp

145 150 155 160

Thr Phe Gly Ser Glu Lys Ala Ile Gln Leu Ile Ala Met Ala Thr Pro

165 170 175

Glu Asp Leu Arg Ile Asn Ala Glu His Ile Arg Ile Ala Asp Gln Phe

180 185 190

Val Glu Val Pro Gly Gly Thr Asn Asn Asn Asn Tyr Ala Asn Val Gln

195 200 205

Leu Ile Val Glu Ile Ala Glu Arg Thr Gly Val Ser Ala Val Trp Pro

210 215 220

Gly Trp Gly His Ala Ser Glu Asn Pro Glu Leu Pro Asp Ala Leu Thr

225 230 235 240

Ala Lys Gly Ile Val Phe Leu Gly Pro Pro Ala Ser Ser Met His Ala

245 250 255

Leu Gly Asp Lys Val Gly Ser Ala Leu Ile Ala Gln Ala Ala Gly Val

260 265 270

Pro Thr Leu Ala Trp Ser Gly Ser His Val Glu Val Pro Leu Glu Cys

275 280 285

Cys Leu Asp Ser Ile Pro Asp Glu Met Tyr Arg Lys Ala Cys Val Thr

290 295 300

Thr Thr Glu Glu Ala Val Ala Ser Cys Gln Val Val Gly Tyr Pro Ala

305 310 315 320

Met Ile Lys Ala Ser Trp Gly Gly Gly Gly Lys Gly Ile Arg Lys Val

325 330 335

His Asn Asp Asp Glu Val Arg Thr Leu Phe Lys Gln Val Gln Gly Glu

340 345 350

Val Pro Gly Ser Pro Ile Phe Ile Met Arg Leu Ala Ala Gln Ser Arg

355 360 365

His Leu Glu Val Gln Leu Leu Cys Asp Gln Tyr Gly Asn Val Ala Ala

370 375 380

Leu His Ser Arg Asp Cys Ser Val Gln Arg Arg His Gln Lys Ile Ile

385 390 395 400

Glu Glu Gly Pro Val Thr Val Ala Pro Arg Glu Thr Val Lys Glu Leu

405 410 415

Glu Gln Ala Ala Arg Arg Leu Ala Lys Ala Val Gly Tyr Val Gly Ala

420 425 430

Ala Thr Val Glu Tyr Leu Tyr Ser Met Glu Thr Gly Glu Tyr Tyr Phe

435 440 445

Leu Glu Leu Asn Pro Arg Leu Gln Val Glu His Pro Val Thr Glu Trp

450 455 460

Ile Ala Glu Val Asn Leu Pro Ala Ala Gln Val Ala Val Gly Met Gly

465 470 475 480

Ile Pro Leu Trp Gln Ile Pro Glu Ile Arg Arg Phe Tyr Gly Met Asn

485 490 495

His Gly Gly Gly Tyr Asp Leu Trp Arg Lys Thr Ala Ala Leu Ala Thr

500 505 510

Pro Phe Asn Phe Asp Glu Val Asp Ser Lys Trp Pro Lys Gly His Cys

515 520 525

Val Ala Val Arg Ile Thr Ser Glu Asp Pro Asp Asp Gly Phe Lys Pro

530 535 540

Thr Gly Gly Lys Val Lys Glu Ile Ser Phe Lys Ser Lys Pro Asn Val

545 550 555 560

Trp Ala Tyr Phe Ser Val Lys Ser Gly Gly Gly Ile His Glu Phe Ala

565 570 575

Asp Ser Gln Phe Gly His Val Phe Ala Tyr Gly Thr Thr Arg Ser Ala

580 585 590

Ala Ile Thr Thr Met Ala Leu Ala Leu Lys Glu Val Gln Ile Arg Gly

595 600 605

Glu Ile His Ser Asn Val Asp Tyr Thr Val Asp Leu Leu Asn Ala Ser

610 615 620

Asp Phe Arg Glu Asn Lys Ile His Thr Gly Trp Leu Asp Thr Arg Ile

625 630 635 640

Ala Met Arg Val Gln Ala Glu Arg Pro Pro Trp Tyr Ile Ser Val Val

645 650 655

Gly Gly Ala Leu Tyr Lys Thr Val Thr Ala Asn Thr Ala Thr Val Ser

660 665 670

Asp Tyr Val Gly Tyr Leu Thr Lys Gly Gln Ile Pro Pro Lys His Ile

675 680 685

Ser Leu Val Tyr Thr Thr Val Ala Leu Asn Ile Asp Gly Lys Lys Tyr

690 695 700

Thr Ile Asp Thr Val Arg Ser Gly His Gly Ser Tyr Arg Leu Arg Met

705 710 715 720

Asn Gly Ser Thr Val Asp Ala Asn Val Gln Ile Leu Cys Asp Gly Gly

725 730 735

Leu Leu Met Gln Leu Asp Gly Asn Ser His Val Ile Tyr Ala Glu Glu

740 745 750

Glu Ala Ser Gly Thr Arg Leu Leu Ile Asp Gly Lys Thr Cys Met Leu

755 760 765

Gln Asn Asp His Asp Pro Ser Lys Leu Leu Ala Glu Thr Pro Cys Lys

770 775 780

Leu Leu Arg Phe Leu Val Ala Asp Gly Ala His Val Asp Ala Asp Val

785 790 795 800

Pro Tyr Ala Glu Val Glu Val Met Lys Met Cys Met Pro Leu Leu Ser

805 810 815

Pro Ala Ser Gly Val Ile His Val Val Met Ser Glu Gly Gln Ala Met

820 825 830

Gln Ala Gly Asp Leu Ile Ala Arg Leu Asp Leu Asp Asp Pro Ser Ala

835 840 845

Val Lys Arg Ala Glu Pro Phe Glu Asp Thr Phe Pro Gln Met Gly Leu

850 855 860

Pro Ile Ala Ala Ser Gly Gln Val His Lys Leu Cys Ala Ala Ser Leu

865 870 875 880

Asn Ala Cys Arg Met Ile Leu Ala Gly Tyr Glu His Asp Ile Asp Lys

885 890 895

Val Val Pro Glu Leu Val Tyr Cys Leu Asp Thr Pro Glu Leu Pro Phe

900 905 910

Leu Gln Trp Glu Glu Leu Met Ser Val Leu Ala Thr Arg Leu Pro Arg

915 920 925

Asn Leu Lys Ser Glu Leu Glu Gly Lys Tyr Glu Glu Tyr Lys Val Lys

930 935 940

Phe Asp Ser Gly Ile Ile Asn Asp Phe Pro Ala Asn Met Leu Arg Val

945 950 955 960

Ile Ile Glu Glu Asn Leu Ala Cys Gly Ser Glu Lys Glu Lys Ala Thr

965 970 975

Asn Glu Arg Leu Val Glu Pro Leu Met Ser Leu Leu Lys Ser Tyr Glu

980 985 990

Gly Gly Arg Glu Ser His Ala His Phe Val Val Lys Ser Leu Phe Glu

995 1000 1005

Glu Tyr Leu Tyr Val Glu Glu Leu Phe Ser Asp Gly Ile Gln Ser Asp

1010 1015 1020

Val Ile Glu Arg Leu Arg Leu Gln His Ser Lys Asp Leu Gln Lys Val

1025 1030 1035 1040

Val Asp Ile Val Leu Ser His Gln Ser Val Arg Asn Lys Thr Lys Leu

1045 1050 1055

Ile Leu Lys Leu Met Glu Ser Leu Val Tyr Pro Asn Pro Ala Ala Tyr

1060 1065 1070

Arg Asp Gln Leu Ile Arg Phe Ser Ser Leu Asn His Lys Ala Tyr Tyr

1075 1080 1085

Lys Leu Ala Leu Lys Ala Ser Glu Leu Leu Glu Gln Thr Lys Leu Ser

1090 1095 1100

Glu Leu Arg Ala Arg Ile Ala Arg Ser Leu Ser Glu Leu Glu Met Phe

1105 1110 1115 1120

Thr Glu Glu Ser Lys Gly Leu Ser Met His Lys Arg Glu Ile Ala Ile

1125 1130 1135

Lys Glu Ser Met Glu Asp Leu Val Thr Ala Pro Leu Pro Val Glu Asp

1140 1145 1150

Ala Leu Ile Ser Leu Phe Asp Cys Ser Asp Thr Thr Val Gln Gln Arg

1155 1160 1165

Val Ile Glu Thr Tyr Ile Ala Arg Leu Tyr Gln Pro His Leu Val Lys

1170 1175 1180

Asp Ser Ile Lys Met Lys Trp Ile Glu Ser Gly Val Ile Ala Leu Trp

1185 1190 1195 1200

Glu Phe Pro Glu Gly His Phe Asp Ala Arg Asn Gly Gly Ala Val Leu

1205 1210 1215

Gly Asp Lys Arg Trp Gly Ala Met Val Ile Val Lys Ser Leu Glu Ser

1220 1225 1230

Leu Ser Met Ala Ile Arg Phe Ala Leu Lys Glu Thr Ser His Tyr Thr

1235 1240 1245

Ser Ser Glu Gly Asn Met Met His Ile Ala Leu Leu Gly Ala Asp Asn

1250 1255 1260

Lys Met His Ile Ile Gln Glu Ser Gly Asp Asp Ala Asp Arg Ile Ala

1265 1270 1275 1280

Lys Leu Pro Leu Ile Leu Lys Asp Asn Val Thr Asp Leu His Ala Ser

1285 1290 1295

Gly Val Lys Thr Ile Ser Phe Ile Val Gln Arg Asp Glu Ala Arg Met

1300 1305 1310

Thr Met Arg Arg Thr Phe Leu Trp Ser Asp Glu Lys Leu Ser Tyr Glu

1315 1320 1325

Glu Glu Pro Ile Leu Arg His Val Glu Pro Pro Leu Ser Ala Leu Leu

1330 1335 1340

Glu Leu Asp Lys Leu Lys Val Lys Gly Tyr Asn Glu Met Lys Tyr Thr

1345 1350 1355 1360

Pro Ser Arg Asp Arg Gln Trp His Ile Tyr Thr Leu Arg Asn Thr Glu

1365 1370 1375

Asn Pro Lys Met Leu His Arg Val Phe Phe Arg Thr Leu Val Arg Gln

1380 1385 1390

Pro Ser Val Ser Asn Lys Phe Ser Ser Gly Gln Ile Gly Asp Met Glu

1395 1400 1405

Val Gly Ser Ala Glu Glu Pro Leu Ser Phe Thr Ser Thr Ser Ile Leu

1410 1415 1420

Arg Ser Leu Met Thr Ala Ile Glu Glu Leu Glu Leu His Ala Ile Arg

1425 1430 1435 1440

Thr Gly His Ser His Met Tyr Leu His Val Leu Lys Glu Gln Lys Leu

1445 1450 1455

Leu Asp Leu Val Pro Val Ser Gly Asn Thr Val Leu Asp Val Gly Gln

1460 1465 1470

Asp Glu Ala Thr Ala Tyr Ser Leu Leu Lys Glu Met Ala Met Lys Ile

1475 1480 1485

His Glu Leu Val Gly Ala Arg Met His His Leu Ser Val Cys Gln Trp

1490 1495 1500

Glu Val Lys Leu Lys Leu Asp Cys Asp Gly Pro Ala Ser Gly Thr Trp

1505 1510 1515 1520

Arg Ile Val Thr Thr Asn Val Thr Ser His Thr Cys Thr Val Asp Ile

1525 1530 1535

Tyr Arg Glu Met Glu Asp Lys Glu Ser Arg Lys Leu Val Tyr His Pro

1540 1545 1550

Ala Thr Pro Ala Ala Gly Pro Leu His Gly Val Ala Leu Asn Asn Pro

1555 1560 1565

Tyr Gln Pro Leu Ser Val Ile Asp Leu Lys Arg Cys Ser Ala Arg Asn

1570 1575 1580

Asn Arg Thr Thr Tyr Cys Tyr Asp Phe Pro Leu Ala Phe Glu Thr Ala

1585 1590 1595 1600

Val Arg Lys Ser Trp Ser Ser Ser Thr Ser Gly Ala Ser Lys Gly Val

1605 1610 1615

Glu Asn Ala Gln Cys Tyr Val Lys Ala Thr Glu Leu Val Phe Ala Asp

1620 1625 1630

Lys His Gly Ser Trp Gly Thr Pro Leu Val Gln Met Asp Arg Pro Ala

1635 1640 1645

Gly Leu Asn Asp Ile Gly Met Val Ala Trp Thr Leu Lys Met Ser Thr

1650 1655 1660

Pro Glu Phe Pro Ser Gly Arg Glu Ile Ile Val Val Ala Asn Asp Ile

1665 1670 1675 1680

Thr Phe Arg Ala Gly Ser Phe Gly Pro Arg Glu Asp Ala Phe Phe Glu

1685 1690 1695

Ala Val Thr Asn Leu Ala Cys Glu Lys Lys Leu Pro Leu Ile Tyr Leu

1700 1705 1710

Ala Ala Asn Ser Gly Ala Arg Ile Gly Ile Ala Asp Glu Val Lys Ser

1715 1720 1725

Cys Phe Arg Val Gly Trp Ser Asp Asp Gly Ser Pro Glu Arg Gly Phe

1730 1735 1740

Gln Tyr Ile Tyr Leu Ser Glu Glu Asp Tyr Ala Arg Ile Gly Thr Ser

1745 1750 1755 1760

Val Ile Ala His Lys Met Gln Leu Asp Ser Gly Glu Ile Arg Trp Val

1765 1770 1775

Ile Asp Ser Val Val Gly Lys Glu Asp Gly Leu Gly Val Glu Asn Ile

1780 1785 1790

His Gly Ser Ala Ala Ile Ala Ser Ala Tyr Ser Arg Ala Tyr Lys Glu

1795 1800 1805

Thr Phe Thr Leu Thr Phe Val Thr Gly Arg Thr Val Gly Ile Gly Ala

1810 1815 1820

Tyr Leu Ala Arg Leu Gly Ile Arg Cys Ile Gln Arg Leu Asp Gln Pro

1825 1830 1835 1840

Ile Ile Leu Thr Gly Tyr Ser Ala Leu Asn Lys Leu Leu Gly Arg Glu

1845 1850 1855

Val Tyr Ser Ser His Met Gln Leu Gly Gly Pro Lys Ile Met Ala Thr

1860 1865 1870

Asn Gly Val Val His Leu Thr Val Ser Asp Asp Leu Glu Gly Val Ser

1875 1880 1885

Asn Ile Leu Arg Trp Leu Ser Tyr Val Pro Ala Tyr Ile Gly Gly Pro

1890 1895 1900

Leu Pro Val Thr Thr Pro Leu Asp Pro Pro Asp Arg Pro Val Ala Tyr

1905 1910 1915 1920

Ile Pro Glu Asn Ser Cys Asp Pro Arg Ala Ala Ile Arg Gly Val Asp

1925 1930 1935

Asp Ser Gln Gly Lys Trp Leu Gly Gly Met Phe Asp Lys Asp Ser Phe

1940 1945 1950

Val Glu Thr Phe Glu Gly Trp Ala Lys Thr Val Val Thr Gly Arg Ala

1955 1960 1965

Lys Leu Gly Gly Ile Pro Val Gly Val Ile Ala Val Glu Thr Gln Thr

1970 1975 1980

Met Met Gln Thr Ile Pro Ala Asp Pro Gly Gln Leu Asp Ser Arg Glu

1985 1990 1995 2000

Gln Ser Val Pro Arg Ala Gly Gln Val Trp Phe Pro Asp Ser Ala Thr

2005 2010 2015

Lys Thr Ala Gln Ala Leu Leu Asp Phe Asn Arg Glu Gly Leu Pro Leu

2020 2025 2030

Phe Ile Leu Ala Asn Trp Arg Gly Phe Ser Gly Gly Gln Arg Asp Leu

2035 2040 2045

Phe Glu Gly Ile Leu Gln Ala Gly Ser Thr Ile Val Glu Asn Leu Arg

2050 2055 2060

Thr Tyr Asn Gln Pro Ala Phe Val Tyr Ile Pro Met Ala Ala Glu Leu

2065 2070 2075 2080

Arg Gly Gly Ala Trp Val Val Val Asp Ser Lys Ile Asn Pro Asp Arg

2085 2090 2095

Ile Glu Cys Tyr Ala Glu Arg Thr Ala Lys Gly Asn Val Leu Glu Pro

2100 2105 2110

Gln Gly Leu Ile Glu Ile Lys Phe Arg Ser Glu Glu Leu Gln Asp Cys

2115 2120 2125

Met Ser Arg Leu Asp Pro Thr Leu Ile Asp Leu Lys Ala Lys Leu Glu

2130 2135 2140

Val Ala Asn Lys Asn Gly Ser Ala Asp Thr Lys Ser Leu Gln Glu Asn

2145 2150 2155 2160

Ile Glu Ala Arg Thr Lys Gln Leu Met Pro Leu Tyr Thr Gln Ile Ala

2165 2170 2175

Ile Arg Phe Ala Glu Leu His Asp Thr Ser Leu Arg Met Ala Ala Lys

2180 2185 2190

Gly Val Ile Lys Lys Val Val Asp Trp Glu Glu Ser Arg Ser Phe Phe

2195 2200 2205

Tyr Lys Arg Leu Arg Arg Arg Ile Ser Glu Asp Val Leu Ala Lys Glu

2210 2215 2220

Ile Arg Ala Val Ala Gly Glu Gln Phe Ser His Gln Pro Ala Ile Glu

2225 2230 2235 2240

Leu Ile Lys Lys Trp Tyr Ser Ala Ser His Ala Ala Glu Trp Asp Asp

2245 2250 2255

Asp Asp Ala Phe Val Ala Trp Met Asp Asn Pro Glu Asn Tyr Lys Asp

2260 2265 2270

Tyr Ile Gln Tyr Leu Lys Ala Gln Arg Val Ser Gln Ser Leu Ser Ser

2275 2280 2285

Leu Ser Asp Ser Ser Ser Asp Leu Gln Ala Leu Pro Gln Gly Leu Ser

2290 2295 2300

Met Leu Leu Asp Lys Met Asp Pro Ser Arg Arg Ala Gln Leu Val Glu

2305 2310 2315 2320

Glu Ile Arg Lys Val Leu Gly

2325

<210> 23

<211> 25

<212> DNA

<213> ACCase野生型序列ACC-CF引物(Artificial Sequence)

<400> 23

<210> 24

<211> 25

<212> DNA

<213> ACCase野生型序列ACC-CR引物(Artificial Sequence)

<400> 24

<210> 25

<211> 28

<212> DNA

<213> ACCase-5387突变位点ACC5387-MutF引物(Artificial Sequence)

<400> 25

<210> 26

<211> 28

<212> DNA

<213> ACCase-5387突变位点ACC5387-MutR引物(Artificial Sequence)

<400> 26

<210> 27

<211> 28

<212> DNA

<213> ACCase-5389突变位点ACC5389-MutF引物(Artificial Sequence)

<400> 27

<210> 28

<211> 28

<212> DNA

<213> ACCase-5389突变位点ACC5389-MutR引物(Artificial Sequence)

<400> 28

<210> 29

<211> 28

<212> DNA

<213> ACCase-5504突变位点ACC5504-MutF引物(Artificial Sequence)

<400> 29

<210> 30

<211> 28

<212> DNA

<213> ACCase-5504突变位点ACC5504-MutR引物(Artificial Sequence)

<400> 30

<210> 31

<211> 28

<212> DNA

<213> ACCase-5623突变位点ACC5623-MutF引物(Artificial Sequence)

<400> 31

<210> 32

<211> 28

<212> DNA

<213> ACCase-5623突变位点ACC5623-MutR引物(Artificial Sequence)

<400> 32

<210> 33

<211> 30

<212> DNA

<213> ACCase-6028突变位点ACC6028-MutF引物(Artificial Sequence)

<400> 33

<210> 34

<211> 29

<212> DNA

<213> ACCase-6028突变位点ACC6028-MutF引物(Artificial Sequence)

<400> 34

<210> 35

<211> 30

<212> DNA

<213> ACCase-6030突变位点ACC6030-MutF引物(Artificial Sequence)

<400> 35

<210> 36

<211> 29

<212> DNA

<213> ACCase-6030突变位点ACC6030-MutR引物(Artificial Sequence)

<400> 36

<210> 37

<211> 28

<212> DNA

<213> ACCase-6149突变位点ACC6149-MutF引物(Artificial Sequence)

<400> 37

<210> 38

<211> 28

<212> DNA

<213> ACCase-6149突变位点ACC6149-MutR引物(Artificial Sequence)

<400> 38

<210> 39

<211> 28

<212> DNA

<213> ACCase-6178突变位点ACC6178 -MutF引物(Artificial Sequence)

<400> 39

<210> 40

<211> 28

<212> DNA

<213> ACCase-6178突变位点ACC6178 -MutR引物(Artificial Sequence)

<400> 40

<210> 41

<211> 28

<212> DNA

<213> ACCase-6209突变位点ACC6209-MutF引物(Artificial Sequence)

<400> 41

<210> 42

<211> 28

<212> DNA

<213> ACCase-6209突变位点ACC6209-MutR引物(Artificial Sequence)

<400> 42

<210> 43

<211> 30

<212> DNA

<213> ACCase-6316突变位点ACC6316-MutF引物(Artificial Sequence)

<400> 43

<210> 44

<211> 28

<212> DNA

<213> ACCase-6316突变位点ACC6316-MutR引物(Artificial Sequence)

<400> 44

Claims

1.一种水稻ACCase突变型蛋白，所述ACCase突变型蛋白的氨基酸序列存在以下任意一种或多种突变：其对应于野生型水稻ACCase的氨基酸序列的第1796位、第1797位、第1835位、第1875位、第2010位、第2050位、第2060位、第2070位和第2106位发生突变。

2.一种水稻ACCase突变型蛋白，包括：

（a）其氨基酸序列选自由SEQ ID NO. 2、SEQ ID NO. 4、SEQ ID NO. 6、SEQ ID NO. 8、SEQ ID NO. 10、SEQ ID NO. 12、SEQ ID NO. 14、SEQ ID NO. 16、SEQ ID NO. 18和SEQ IDNO. 20组成的组中；或

（b）在（a）中的氨基酸序列经过取代和/或缺失和/或添加一个或多个氨基酸，且具有乙酰辅酶A羧化酶活性的由（a）衍生的蛋白质。

3.一种核酸或基因，其编码根据权利要求1~2任一项所述的蛋白。

4.根据权利要求3所述的核酸或基因，包括：

（i）其编码权利要求1~2任一项所述的蛋白；或

（ii）在严格条件下与（a）限定的核苷酸序列杂交且编码具有乙酰辅酶A羧化酶活性的蛋白质的核苷酸序列；或

（iii）其核苷酸序列选自由SEQ ID NO:1、SEQ ID NO:3、SEQ ID NO:5、SEQ ID NO:7、SEQ ID NO:9、SEQ ID NO:11、SEQ ID NO:13、SEQ ID NO:15、SEQ ID NO:17 和SEQ ID NO:19组成的组中。

5.一种表达盒、重组载体或细胞，其含有根据权利要求3或4所述的核酸或基因。

6.根据权利要求1或2所述的水稻ACCase突变型蛋白、根据权利要求3或4所述的核酸或基因，权利要求5所述的表达盒、重组载体或细胞在植物抗除草剂方面的应用。

7.根据权利要求6所述的应用，其特征在于，所述除草剂是喹禾灵、吡氟氯禾灵、烯草酮、吡氟禾草灵中的一种或多种。

8.一种获得具有除草剂抗性的植物的方法，其特征在于，包括如下步骤：

1）使植物包含根据权利要求3或4所述的核酸或基因；或

2）使植物表达根据权利要求1或2所述的水稻ACCase突变型蛋白。

9.根据权利要求8所述的方法，其特征在于，其包括crispr基因编辑、转基因、杂交、回交或无性繁殖步骤。

10.一种鉴定植物的方法，其中所述植物是包含根据权利要求3或4所述的核酸或基因的植物、表达根据权利要求1~2之任一所述的蛋白的植物或由根据权利要求8~9之任一所述的方法获得的植物，其特征在于，包括以下步骤：

1) 测定所述植物是否包含根据权利要3或4所述的核酸或基因；或，

2) 测定所述植物是否表达根据权利要求1~2之任一所述的蛋白。

11.一种控制杂草的方法，其特征在于，包括：对种植作物的大田施用有效剂量的除草剂，所述作物包含根据权利要求3或4所述的核酸或基因或根据权利要求5所述的表达盒、重组载体或细胞，所述除草剂为喹禾灵、吡氟氯禾灵、烯草酮、吡氟禾草灵中的一种或多种。

12.一种用于保护植物免受由除草剂引起的损伤的方法，其特征在于，包括：对种植作物的大田施用有效剂量的除草剂，所述作物包含根据权利要求3或4所述的核酸或基因或将权利要求5所述的表达盒、重组载体导入植物，导入后的植物产生除草剂抗性蛋白，所述除草剂为喹禾灵、吡氟氯禾灵、烯草酮、吡氟禾草灵中的一种或多种。