CN109079947A - A kind of stained wood for the method and preparation that wood staining is handled - Google Patents
A kind of stained wood for the method and preparation that wood staining is handled Download PDFInfo
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- CN109079947A CN109079947A CN201810882048.8A CN201810882048A CN109079947A CN 109079947 A CN109079947 A CN 109079947A CN 201810882048 A CN201810882048 A CN 201810882048A CN 109079947 A CN109079947 A CN 109079947A
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B27—WORKING OR PRESERVING WOOD OR SIMILAR MATERIAL; NAILING OR STAPLING MACHINES IN GENERAL
- B27K—PROCESSES, APPARATUS OR SELECTION OF SUBSTANCES FOR IMPREGNATING, STAINING, DYEING, BLEACHING OF WOOD OR SIMILAR MATERIALS, OR TREATING OF WOOD OR SIMILAR MATERIALS WITH PERMEANT LIQUIDS, NOT OTHERWISE PROVIDED FOR; CHEMICAL OR PHYSICAL TREATMENT OF CORK, CANE, REED, STRAW OR SIMILAR MATERIALS
- B27K5/00—Treating of wood not provided for in groups B27K1/00, B27K3/00
- B27K5/02—Staining or dyeing wood; Bleaching wood
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Abstract
The invention discloses a kind of timber biological dyeing treatment and stained wood, wood staining method of the invention includes the chromogenic plain microorganism of surface seeding in timber, using the growth of chromogenic plain microorganism, induction dyeing culture is carried out to timber, so that stain for wood.The stained wood colour fixation prepared using the method for the present invention is good, operating method is simple, dyeing condition is mild, and stained wood is not easy to change, decorative pattern is controllable, effectively prevents environmental problem brought by the leachability of chemical dye in the existing timber using chemical dye dyeing, the method for the present invention is to person poultry harmless, a variety of timber can be dyed, the production cost of the stained wood of preparation is low.
Description
Technical field
The present invention relates to a kind of methods dyed using Botryodiplodia theo-bromae bacterium to timber biological, belong to Wood Dyeing Process neck
Domain.
Technical background
Botryodiplodia theo-bromae bacterium (Botryodiplodia thebromae Pat.) is a kind of stain fungus, can pass through spore
Mycelia enters timber by conduit or horizontal organization after breeding, air borne and spore-germination, so that wood internal becomes blue
Color or black.According to " pathogenic microorganism Laboratory biosafety management rules ", Botryodiplodia theo-bromae Pseudomonas is in the 4th class cause of disease
Microorganism will not cause the mankind or Animal diseases in general.Botryodiplodia theo-bromae Pseudomonas Fungi Imperfecti, Sphaeropsidales,
Pycnidia, full maturity after 15 days can be formed after 10 days in plating medium.The mycelia of Botryodiplodia theo-bromae bacterium is sturdy, shows
In coffee color under micro mirror, it is distributed mainly on sapwood parenchymal tissue, fiber and catheter lumen, when nutrition is abundant, can be also diffused into whole
A xylem, but mycelia is very thin, does not influence substantially on the mechanical property of timber.
Research thinks, the melanin deposition and the mycelia institute around it that Botryodiplodia theo-bromae bacterium makes rubber wood timber discoloration be its secretion
It causes.The culture mediums that difference is crossed, will affect the secretion of Botryodiplodia theo-bromae bacterium pigment, infect timber using Botryodiplodia theo-bromae bacterium, can be with
Making to be infected sapstain brown, blue or black, the timber after changing colour has higher ornamental value than common wood, and
And it overcomes using chemical synthetic dye to the problem of environmental pollution of wood staining and the dyestuff losing issue of chemical staining timber.
Summary of the invention
The purpose of the present invention is for existing wood staining handle, provide it is a kind of using microorganism can
Can two spore bacterium of ball to timber carry out biology induction dyeing method and using the colouring method preparation stained wood, side of the present invention
Method carries out biological stain processing to timber using Botryodiplodia theo-bromae bacterium, and Botryodiplodia theo-bromae bacterium carries out microorganism on timber to be dyed
Breeding, chromogenesis during growth metabolism dye timber.The method of the present invention operating method is simple, the dye of preparation
The fixed jail of color wood color, avoids pigment loss, avoids the loss of chemical dye and paints the influence to environment, can be right
It is mostly dyed in timber, stained wood low production cost.
To achieve the purpose of the present invention, one aspect of the present invention provides a kind of wood staining processing method, is included in be dyed
The chromogenic plain microorganism of surface seeding for handling timber, carries out induction dyeing culture.
Chromogenic element microorganism is bred in wood surface, a large amount of pigments is generated in growth course, by wood staining.
Wherein, the timber selection rubber wood timber, birch, masson pine or eucalyptus citriodora, preferably rubber wood timber.
In particular, the chromogenic plain microorganism is Botryodiplodia theo-bromae bacterium (Botryodiplodia thebromae Pat.).
Wherein, induction dyeing culture includes the following steps: Botryodiplodia theo-bromae bacterium in expanding on solid medium
Numerous culture；Then by expand the Botryodiplodia theo-bromae bacterium mycelia after numerous culture then and be covered on processing to be dyed timber surface,
Carry out microbial reproduction culture.
After the spore-germination of Botryodiplodia theo-bromae bacterium, mycelia can be entered in timber by the conduit or horizontal organization of timber
Portion is distributed in the parenchymal tissue of timber, fiber and catheter lumen, and then is diffused into entire xylem, and the metabolism of Botryodiplodia theo-bromae bacterium produces
Raw melanin can be secreted by mycelia, and then timber is made to change colour.
In particular, the condition of culture of the induction dyeing culture are as follows: cultivation temperature is 20-35 DEG C, preferably 25-30 DEG C；
Incubation time is 5-20 days, preferably 6-15 days, further preferably 10-15 days.
Especially, in the induction dyeing incubation, control relative humidity is 24-96%.
In particular, the inoculum concentration of the Botryodiplodia theo-bromae bacterium is the fresh mycelia 0.5-2.0g of inoculation every square centimeter, preferably
For 0.7-1.5g.
Wherein, the solid medium for expanding numerous culture Botryodiplodia theo-bromae bacterium selects carbon-nitrogen ratio for the culture of 7:1-20:1
Base.
In particular, the solid medium selection is formulated following culture medium 1: ammonium sulfate 1-4g, dipotassium hydrogen phosphate 1g, chlorine
Change potassium 0.5g, magnesium sulfate 0.5g, ferrous sulfate 0.01g, sucrose 10-40g, agar 15-20g, water 1000mL.
In particular, the solid medium selection is formulated following culture medium 2: beef extract 1-10g, dipotassium hydrogen phosphate 1g,
Potassium chloride 0.5g, magnesium sulfate 0.5g, ferrous sulfate 0.01g, sucrose 10-40g, agar 15-20g, water 1000mL.
In particular, the solid medium selection is formulated following culture medium 3: peptone 1-10g, dipotassium hydrogen phosphate 1g,
Potassium chloride 0.5g, magnesium sulfate 0.5g, ferrous sulfate 0.01g, sucrose 10-40g, agar 15-20g, water 1000mL.
Wherein, it is described expand numerous culture be chromogenic plain microorganism Botryodiplodia theo-bromae bacterium is seeded on solid medium cultivate to
Botryodiplodia theo-bromae bacterium mycelia is covered on culture medium.
In particular, the condition of culture for expanding numerous culture are as follows: cultivation temperature is 20-35 DEG C, preferably 25-30 DEG C；Culture
Time is 5-20 days, preferably 7-15 days.
Especially, described to expand in numerous incubation, control relative humidity is 24-92%.
In particular, the processing timber to be dyed after high pressure steam sterilization is handled, is inoculated with chromogenic then at wood surface
Plain microorganism carries out the induction dyeing culture processing.
Wherein, processing timber to be dyed carries out high pressure steam sterilization processing, and the purpose is to reduce other, there may be miscellaneous bacterias
Interference to chromogenic plain microorganism growth, influences wood staining effect.
In particular, the high pressure steam sterilization processing is the sterilizing described using progress in high-pressure steam sterilizing pan.
In particular, the temperature of the high pressure steam sterilization processing is (121.3 ± 2) DEG C；Pressure (103.4 ± 10) kPa；It goes out
The bacterium time is (30 ± 5) min.
Especially, the timber after high pressure steam sterilization, after carrying out constant temperature and humidity processing, then in the surface seeding of timber
The culture of chromogenic element microorganism Botryodiplodia theo-bromae bacterium carries out induction dyeing culture processing.
Wherein, the constant temperature and humidity condition are as follows: temperature is 25-35 DEG C；Relative humidity is 24%-96%.
In particular, the constant temperature and humidity condition are as follows: temperature is 25-30 DEG C.
Especially, being inoculated with the inoculum concentration of chromogenic plain microorganism Botryodiplodia theo-bromae bacterium in wood surface is inoculation every square centimeter
Fresh mycelia 0.5-1.5g, preferably 0.7-1.5g.
In particular, further including carrying out sterilization treatment to induction dyeing culture treated timber.
Especially, the sterilization treatment is handled using high pressure steam sterilization, and sterilization treatment temperature is (121.3 ± 2) DEG C；Pressure
Power (103.4 ± 10) kPa；Sterilization time is (30 ± 5) min.
Another aspect of the present invention provides a kind of stained wood being prepared according to above-mentioned colouring method.
Wherein, the timber selection rubber wood timber, birch, masson pine or eucalyptus citriodora, preferably rubber wood timber.
Compared with prior art, the present invention has the advantage that
1, the method for the present invention can carry out biological stain, especially best to rubber wood timber dyeing effect, dyeing to a variety of tree species
Quality is stablized.
2, the present invention is easy to operate using the method for Botryodiplodia theo-bromae bacterium biological stain, low in cost, and color fastness is high, is not easy
Discoloration, decorative pattern is controllable, effectively prevents the decorative timbers mode such as the leachability of chemical staining dyestuff in wood and paint and brings
Environmental problem, it is environmentally protective and to person poultry harmless.
3, the method for the present invention is simple to timber progress biological stain processing operation method, and treatment conditions are mild, can be to more
Kind timber carries out biological stain.
Specific embodiment
The invention will now be further described with reference to specific embodiments, the advantages and features of the present invention will be with description and
It is apparent.But examples are merely exemplary for these, and it is not intended to limit the scope of the present invention in any way.Those skilled in the art
Member it should be understood that without departing from the spirit and scope of the invention can details to technical solution of the present invention and form into
Row modifications or substitutions, but these modifications and replacement are fallen within the protection scope of the present invention.
Embodiment 1
1, test material
Chromogenic plain microorganism Botryodiplodia theo-bromae bacterium (Botryodiplodia thebromae Pat.) of the invention is purchased from north
Na Chuanlian Bioisystech Co., Ltd, it is spare in being refrigerated in 4 DEG C of refrigerator.
2, culture medium is prepared
1) culture medium 1:
Ammonium sulfate 1-4g, dipotassium hydrogen phosphate 1g, potassium chloride 0.5g, magnesium sulfate 0.5g, ferrous sulfate 0.01g, sucrose 10-
40g, agar 15-20g, water 1000mL.
2) culture medium 2:
Beef extract 1-10g, dipotassium hydrogen phosphate 1g, potassium chloride 0.5g, magnesium sulfate 0.5g, ferrous sulfate 0.01g, sucrose 10-
40g, agar 15-20g, water 1000mL.
3) culture medium 3:
Peptone 1-10g, dipotassium hydrogen phosphate 1g, potassium chloride 0.5g, magnesium sulfate 0.5g, ferrous sulfate 0.01g, sucrose 10-
40g, agar 15-20g, water 1000mL.
Above-mentioned culture medium weighs required agar, is poured into culture volume 3/4 to be matched according to the quantity of configuration culture medium
Sterile water in, be added each ingredient of culture medium, every a kind of ingredient is added all to be sufficiently stirred, finally plus water is settled to culture medium most
Final volume, culture medium constant temperature at 121 DEG C sterilize 15 minutes.After above-mentioned culture medium prepares, it is stand-by to be stored in 4 DEG C of refrigerators.
Embodiment 2
The method dyed using Botryodiplodia theo-bromae bacterium to timber biological of the present embodiment, is included the following steps:
1, timber pre-processes
It treats stained wood rubber wood timber and is placed in progress high pressure steam sterilization processing in high-pressure steam sterilizing pan, wherein high pressure
In steam sterilizing treatment process, control sterilization treatment (30 ± 5) min under conditions of sterilising temp is (121.3 ± 2) DEG C, system
Must sterilize timber；
Sterilizing timber is placed in climatic chamber, in 30 DEG C (usually 25-35 DEG C), relative humidity is 35% (usual
To carry out constant temperature and humidity processing under conditions of 24%-96%), handling to the temperature of timber is 30 DEG C (usually 25-35 DEG C)；Contain
Water rate is 35% (usually 24-96%), obtains pretreatment timber, spare.
Constant temperature and humidity handle it is identical as the growing environment of Botryodiplodia theo-bromae bacterium to the temperature and humidity of timber, be suitable for cocoa ball
The growth of two spore bacterium, i.e., it is consistent with the optimum growth temperature of Botryodiplodia theo-bromae bacterium, humidity.
2, the numerous culture of the expansion of Botryodiplodia theo-bromae bacterium
Chromogenic plain microorganism Botryodiplodia theo-bromae bacterium is seeded on culture medium 1, carries out expanding in constant temperature and humidity incubator numerous
Culture, the purpose and effect for expanding numerous culture are: a large amount of Botryodiplodia theo-bromae bacterium are cultivated, in order to there is enough bacterium to be inoculated into timber
On；Wherein, expand in numerous incubation that control cultivation temperature be 27 DEG C (usually 25-35 DEG C), relative humidity is 35% (usually
24-96%), Botryodiplodia theo-bromae bacterium mycelia is covered on culture to culture medium, being generally incubated the time is 5-20 days, obtains and expands numerous culture
Chromogenic plain microorganism (i.e. a large amount of fresh Botryodiplodia theo-bromae bacterium mycelia), the wherein formula of culture medium 1: ammonium sulfate 2g, phosphoric acid hydrogen
Dipotassium 1g, potassium chloride 0.5g, magnesium sulfate 0.5g, ferrous sulfate 0.01g, sucrose 15g, agar 15g, water 1000mL；Culture medium 1
Carbon-nitrogen ratio is 15:1.
It is illustrated so that the carbon-nitrogen ratio of culture medium 1 is 15:1 as an example in the embodiment of the present invention, other carbon-nitrogen ratios are 7-20:
1 culture medium 1 is also applied for the present invention.
3, induction dyeing processing
The Botryodiplodia theo-bromae bacterium mycelia for expanding numerous culture (i.e. chromogenic plain microorganism) is inoculated with and is covered on the pre- of step 1 preparation
Handle the surface of timber；In 27 DEG C (usually 25-35 DEG C), relative humidity be 35% (usually 24-96%) under conditions of into
Row microculture；Botryodiplodia theo-bromae bacterium flourish on rubber wood timber, mycelia enter timber by conduit or horizontal organization,
So that wood surface and intrinsic stain；Wherein, the inoculum concentration of Botryodiplodia theo-bromae bacterium is that wood surface inoculation every square centimeter is new
Fresh Botryodiplodia theo-bromae bacterium mycelia 0.7g；
In the Botryodiplodia theo-bromae bacterium of culture 10 days (usually 5-20 days) removal wood surface；Obtain induction dyeing processing wood
Material；
The present invention, which is inoculated with Botryodiplodia theo-bromae bacterium, can be seeded in Botryodiplodia theo-bromae bacterium entire wood surface or basis
The demand of pigmented section or size, the shape etc. for dyeing pattern are inoculated in corresponding region.
The present invention induces in dyeing treatment process chromogenic plain microorganism to expand numerous culture mix can be by timber to be dyed
Surface all cover, can also with covering part, can also wood surface cover at different shapes, decorative pattern, wood surface
In microorganism growth process, the pigment that microorganism generates is according to the covering shape, size, decorative pattern of the numerous culture mix of expansion to timber
It is dyed, forms different dyeing shapes, size, decorative pattern.
4, timber sterilizing, drying process
Processing timber is dyed into induction and is placed in progress high pressure steam sterilization processing in high-pressure steam sterilizing pan, wherein high pressure
In steam sterilizing treatment process, control sterilising temp is (121.3 ± 2) DEG C, sterilization treatment (30 ± 5) min；It then will be at sterilizing
Timber after reason is dried, and obtains stained wood, and stained wood is surface rubber wood timber blue.
Embodiment 2A
Culture medium Selective agar medium 2 in addition to expanding numerous culture in the numerous incubation step of expansion of step 2) Botryodiplodia theo-bromae bacterium, step
The inoculum concentration of Botryodiplodia theo-bromae bacterium is that wood surface every square centimeter is inoculated with fresh cocoa in rapid 3) induction dyeing processing supplement
Except two spore bacterium mycelia 0.8g of ball, remaining is same as Example 2, wherein the formula of culture medium 2 are as follows: beef extract 4g, phosphoric acid hydrogen two
Potassium 1g, potassium chloride 0.5g, magnesium sulfate 0.5g, ferrous sulfate 0.01g, sucrose 30g, agar 20g, water 1000mL；The carbon of culture medium 2
Nitrogen ratio is 20:1.
Stained wood obtained is surface dyeing rubber wood timber blue.
Embodiment 2B
Culture medium Selective agar medium 2 in addition to expanding numerous culture in the numerous incubation step of expansion of step 2) Botryodiplodia theo-bromae bacterium, step
The inoculum concentration of Botryodiplodia theo-bromae bacterium is that wood surface every square centimeter is inoculated with fresh cocoa in rapid 3) induction dyeing processing supplement
Except two spore bacterium mycelia 0.8g of ball, remaining is same as Example 2, wherein the formula of culture medium 3 are as follows: peptone 4g, phosphoric acid hydrogen two
Potassium 1g, potassium chloride 0.5g, magnesium sulfate 0.5g, ferrous sulfate 0.01g, sucrose 30g, agar 20g, water 1000mL；The carbon of culture medium 3
Nitrogen ratio is 20:1.
Stained wood obtained is that surface is in nattier blue dyeing rubber wood timber.
Embodiment 3
1, timber pre-processes
It treats stained wood rubber wood timber and is placed in progress high pressure steam sterilization processing in high-pressure steam sterilizing pan, wherein high pressure
In steam sterilizing treatment process, control sterilization treatment (30 ± 5) min under conditions of sterilising temp is (121.3 ± 2) DEG C, system
Must sterilize timber；
Sterilizing timber is placed in climatic chamber, in 30 DEG C (usually 25-35 DEG C), relative humidity is 65% (usual
To carry out constant temperature and humidity processing under conditions of 24%-96%), handling to the temperature of timber is 30 DEG C (usually 25-35 DEG C)；Contain
Water rate is 65% (usually 24-96%), obtains pretreatment timber, spare.
2, the numerous culture of the expansion of Botryodiplodia theo-bromae bacterium
Chromogenic plain microorganism Botryodiplodia theo-bromae bacterium is seeded on culture medium 2, carries out expanding in constant temperature and humidity incubator numerous
Culture, wherein expanding control cultivation temperature in numerous incubation is 25 DEG C (usually 25-35 DEG C), and relative humidity is 65% (usual
For 24-96%), Botryodiplodia theo-bromae bacterium mycelia is covered in culture to culture medium, being generally incubated the time is 5-20 days, obtains the numerous training of expansion
Feeding chromogenic plain microorganism (i.e. a large amount of Botryodiplodia theo-bromae bacterium mycelia), the wherein formula of culture medium 2: beef extract 4g, phosphoric acid hydrogen two
Potassium 1g, potassium chloride 0.5g, magnesium sulfate 0.5g, ferrous sulfate 0.01g, sucrose 30g, agar 20g, water 1000mL；The carbon of culture medium 2
Nitrogen ratio is 20:1.
It is illustrated so that the carbon-nitrogen ratio of culture medium 2 is 20:1 as an example in the embodiment of the present invention, other carbon-nitrogen ratios are 7-20:
1 culture medium 2 is also applied for the present invention.
3, induction dyeing processing
The Botryodiplodia theo-bromae bacterium mycelia for expanding numerous culture (i.e. chromogenic plain microorganism) is inoculated with and is covered on the pre- of step 1 preparation
Handle the surface of timber；In 25 DEG C (usually 25-35 DEG C), relative humidity be 65% (usually 24-96%) under conditions of into
Row microculture；Botryodiplodia theo-bromae bacterium flourish on rubber wood timber, mycelia enter timber by conduit or horizontal organization,
So that wood surface and intrinsic stain；Wherein, the inoculum concentration of Botryodiplodia theo-bromae bacterium is that wood surface inoculation every square centimeter is new
Fresh Botryodiplodia theo-bromae bacterium mycelia 1.2g；
In the Botryodiplodia theo-bromae bacterium of culture 14 days (usually 5-20 days) removal wood surface；Obtain induction dyeing processing wood
Material；
4, timber sterilizing, drying process
Processing timber is dyed into induction and is placed in progress high pressure steam sterilization processing in high-pressure steam sterilizing pan, wherein high pressure
In steam sterilizing treatment process, control sterilising temp is (121.3 ± 2) DEG C, sterilization treatment (30 ± 5) min；It then will be at sterilizing
Timber after reason is dried, and obtains stained wood, and stained wood is that surface is in nattier blue dyeing rubber wood timber.
Embodiment 3A
Culture medium Selective agar medium 3 in addition to expanding numerous culture in the numerous incubation step of expansion of step 2) Botryodiplodia theo-bromae bacterium, step
The inoculum concentration of Botryodiplodia theo-bromae bacterium is that wood surface every square centimeter is inoculated with fresh cocoa in rapid 3) induction dyeing processing supplement
Except two spore bacterium mycelia 1.2g of ball, remaining is same as Example 3, wherein the formula of culture medium 3 are as follows: peptone 10g, phosphoric acid hydrogen two
Potassium 1g, potassium chloride 0.5g, magnesium sulfate 0.5g, ferrous sulfate 0.01g, sucrose 30g, agar 20g, water 1000mL；The carbon of culture medium 3
Nitrogen ratio is 10:1.
Stained wood obtained is the dyeing rubber wood timber that surface is in brown.
Embodiment 3B
Culture medium Selective agar medium 1 in addition to expanding numerous culture in the numerous incubation step of expansion of step 2) Botryodiplodia theo-bromae bacterium, step
The inoculum concentration of Botryodiplodia theo-bromae bacterium is that wood surface every square centimeter is inoculated with fresh cocoa in rapid 3) induction dyeing processing supplement
Except two spore bacterium mycelia 1.2g of ball, remaining is same as Example 3, wherein the formula of culture medium 1 are as follows: ammonium sulfate 6g, phosphoric acid hydrogen two
Potassium 1g, potassium chloride 0.5g, magnesium sulfate 0.5g, ferrous sulfate 0.01g, sucrose 30g, agar 15g, water 1000mL；The carbon of culture medium 1
Nitrogen ratio is 13:1.
Stained wood obtained is surface dyeing rubber wood timber blue.
Embodiment 4
1, timber pre-processes
It treats stained wood birch and is placed in progress high pressure steam sterilization processing in high-pressure steam sterilizing pan, wherein high pressure is steamed
In vapour sterilisation process, control sterilization treatment (30 ± 5) min under conditions of sterilising temp is (121.3 ± 2) DEG C is made
Sterilize timber；
Sterilizing timber is placed in climatic chamber, in 30 DEG C (usually 25-35 DEG C), relative humidity is 96% (usual
To carry out constant temperature and humidity processing under conditions of 24%-96%), handling to the temperature of timber is 30 DEG C (usually 25-35 DEG C)；Contain
Water rate is 96% (usually 24-96%), obtains pretreatment timber, spare.
2, the numerous culture of the expansion of Botryodiplodia theo-bromae bacterium
Chromogenic plain microorganism Botryodiplodia theo-bromae bacterium is seeded on culture medium 3, carries out expanding in constant temperature and humidity incubator numerous
Culture, wherein expanding control cultivation temperature in numerous incubation is 30 DEG C (usually 25-35 DEG C), and relative humidity is 96% (usual
For 24-96%), Botryodiplodia theo-bromae bacterium mycelia is covered in culture to culture medium, being generally incubated the time is 5-20 days, obtains the numerous training of expansion
Feeding chromogenic plain microorganism (i.e. a large amount of Botryodiplodia theo-bromae bacterium mycelia), the wherein formula of culture medium 3: peptone 4g, phosphoric acid hydrogen two
Potassium 1g, potassium chloride 0.5g, magnesium sulfate 0.5g, ferrous sulfate 0.01g, sucrose 30g, agar 20g, water 1000mL；The carbon of culture medium 3
Nitrogen ratio is 20:1.
It is illustrated so that the carbon-nitrogen ratio of culture medium 3 is 20:1 as an example in the embodiment of the present invention, other carbon-nitrogen ratios are 7-20:
1 culture medium 3 is also applied for the present invention.
3, induction dyeing processing
The Botryodiplodia theo-bromae bacterium mycelia for expanding numerous culture (i.e. chromogenic plain microorganism) is inoculated with and is covered on the pre- of step 1 preparation
Handle the surface of timber；In 30 DEG C (usually 25-35 DEG C), relative humidity be 96% (usually 24-96%) under conditions of into
Row microculture；Botryodiplodia theo-bromae bacterium flourish on birch, mycelia enter timber by conduit or horizontal organization, make
Obtain wood surface and intrinsic stain；Wherein, the inoculum concentration of Botryodiplodia theo-bromae bacterium is that wood surface inoculation every square centimeter is fresh
Botryodiplodia theo-bromae bacterium mycelia 1.5g；
In the Botryodiplodia theo-bromae bacterium of culture 13 days (usually 5-20 days) removal wood surface；Obtain induction dyeing processing wood
Material；
4, timber sterilizing, drying process
Processing timber is dyed into induction and is placed in progress high pressure steam sterilization processing in high-pressure steam sterilizing pan, wherein high pressure
In steam sterilizing treatment process, control sterilising temp is (121.3 ± 2) DEG C, sterilization treatment (30 ± 5) min；It then will be at sterilizing
Timber after reason is dried, and obtains stained wood, and stained wood is that surface is in nattier blue dyeing birch.
Embodiment 4A
Culture medium Selective agar medium 2 in addition to expanding numerous culture in the numerous incubation step of expansion of step 2) Botryodiplodia theo-bromae bacterium, step
The inoculum concentration of Botryodiplodia theo-bromae bacterium is that wood surface every square centimeter is inoculated with fresh cocoa in rapid 3) induction dyeing processing supplement
Except two spore bacterium mycelia 1.5g of ball, remaining is same as Example 4, wherein the formula of culture medium 2 are as follows: beef extract 10g, phosphoric acid hydrogen two
Potassium 1g, potassium chloride 0.5g, magnesium sulfate 0.5g, ferrous sulfate 0.01g, sucrose 30g, agar 20g, water 1000mL；The carbon of culture medium 2
Nitrogen ratio is 13:1.
Stained wood obtained is surface dyeing birch blue.
Embodiment 4B
Culture medium Selective agar medium 1 in addition to expanding numerous culture in the numerous incubation step of expansion of step 2) Botryodiplodia theo-bromae bacterium, step
The inoculum concentration of Botryodiplodia theo-bromae bacterium is that wood surface every square centimeter is inoculated with fresh cocoa in rapid 3) induction dyeing processing supplement
Except two spore bacterium mycelia 1.5g of ball, remaining is same as Example 4, wherein the formula of culture medium 1 are as follows: ammonium sulfate 1g, phosphoric acid hydrogen two
Potassium 1g, potassium chloride 0.5g, magnesium sulfate 0.5g, ferrous sulfate 0.01g, sucrose 30g, agar 15g, water 1000mL；The carbon of culture medium 1
Nitrogen ratio is 13:1.
Stained wood obtained is that surface is in nattier blue dyeing birch.
Claims (10)
1. a kind of wood staining processing method, characterized in that the chromogenic element of surface seeding including the timber in processing to be dyed is micro-
Biology carries out induction dyeing culture.
2. the method as described in claim 1, characterized in that the chromogenic plain microorganism is Botryodiplodia theo-bromae bacterium
(Botryodiplodia thebromae Pat.)。
3. method according to claim 2, characterized in that the induction dyeing culture includes the following steps: cocoa ball two
Spore bacterium on solid medium in carrying out expanding numerous culture；Then it will expand the Botryodiplodia theo-bromae bacterium mycelium inoculation after numerous culture and be covered on
The surface of the timber of processing to be dyed carries out microbial reproduction culture.
4. method a method according to any one of claims 1-3, characterized in that the processing timber to be dyed passes through high pressure steam sterilization
Afterwards, the chromogenic plain microorganism is inoculated.
5. method as claimed in claim 3, characterized in that the solid medium for expanding numerous culture selects carbon-nitrogen ratio for 7-
The culture medium of 20:1.
6. method as claimed in claim 3, characterized in that the solid medium selection is formulated following culture medium 1: sulfuric acid
Ammonium 1-4g, dipotassium hydrogen phosphate 1g, potassium chloride 0.5g, magnesium sulfate 0.5g, ferrous sulfate 0.01g, sucrose 10-40g, agar 15-
20g, water 1000mL.
7. method according to claim 1 or 2, characterized in that selection is formulated following culture medium 2 in the solid culture:
Beef extract 1-10g, dipotassium hydrogen phosphate 1g, potassium chloride 0.5g, magnesium sulfate 0.5g, ferrous sulfate 0.01g, sucrose 10-40g, agar
15-20g, water 1000mL.
8. method according to claim 1 or 2, characterized in that the solid medium selection is formulated following culture medium 3:
Peptone 1-10g, dipotassium hydrogen phosphate 1g, potassium chloride 0.5g, magnesium sulfate 0.5g, ferrous sulfate 0.01g, sucrose 10-40g, agar
15-20g, water 1000mL.
9. such as any the method for claim 1-3, characterized in that the condition of culture of the induction dyeing culture are as follows: be protected from light, train
Supporting temperature is 20-35 DEG C；Incubation time is 5-20 days.
10. a kind of stained wood, characterized in that be prepared according to such as any the method for claim 1-9.
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| CN111113604A (en) * | 2019-12-30 | 2020-05-08 | 北京林业大学 | Method for cooperatively staining wood by chromobacterium in segmented mode |
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