CN109077199A - Vegetables mitochondria oral emulsion and preparation method thereof - Google Patents

Vegetables mitochondria oral emulsion and preparation method thereof Download PDF

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Publication number
CN109077199A
CN109077199A CN201710449222.5A CN201710449222A CN109077199A CN 109077199 A CN109077199 A CN 109077199A CN 201710449222 A CN201710449222 A CN 201710449222A CN 109077199 A CN109077199 A CN 109077199A
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China
Prior art keywords
mitochondria
beverage
oral
pressing
vegetables
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CN201710449222.5A
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Chinese (zh)
Inventor
石正国
丁恩友
石政涛
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Fa Technology (guizhou) Co Ltd
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Fa Technology (guizhou) Co Ltd
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Priority to CN201710449222.5A priority Critical patent/CN109077199A/en
Publication of CN109077199A publication Critical patent/CN109077199A/en
Pending legal-status Critical Current

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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/02Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation containing fruit or vegetable juices
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/52Adding ingredients
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/52Adding ingredients
    • A23L2/60Sweeteners
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/105Plant extracts, their artificial duplicates or their derivatives
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/115Fatty acids or derivatives thereof; Fats or oils
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/125Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives containing carbohydrate syrups; containing sugars; containing sugar alcohols; containing starch hydrolysates
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/15Vitamins
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Polymers & Plastics (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Nutrition Science (AREA)
  • Mycology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Molecular Biology (AREA)
  • Botany (AREA)
  • Non-Alcoholic Beverages (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)

Abstract

The present invention provides a kind of using vegetables as the oral emulsion production technology rich in mitochondria of raw material.Mitochondria is intracellular energy generation mechanism, by supplementing mitochondria, can generate more energy for body, strengthen the metabolism of tissue.Then the present invention is aided with liposome preparatory techniques by sucrose differential centrifugation separate mitochondria, to isolated one layer of bimolecular film protection of mitochondria, form the oral milk product of pure taste.

Description

Vegetables mitochondria oral emulsion and preparation method thereof
Technical field
This patent provides a kind of processing of agriculture product technology, based on the deep processing of agricultural and sideline product, with modern industry Production technology combines, and provides a kind of body energy supplement beverage based on organelle " mitochondria ".
Background technique
Exercise makes one strong, and moves and the energy is needed to guarantee, and mitochondria is to provide the pass of the energy safeguard of vital movement Key organelle.Supplement mitochondria is the shortcut of effective offer life entity energy safeguard.It is reported that relatively being done more physical exercises it Afterwards, intracellular mitochondria is than having apparent increase in the state of peace and quiet.For from another level, pass through supplementary cords grain Body is exactly the reliable energy provision level of the raising body of real meaning.
The product of country's beverage market is mainly the supplement beverage of tea beverage and general micro-element or minerals at present, is led to Replenishing vitamins are crossed to supplement the nutrition of human body.Correspondingly, a large amount of agricultural and sideline product raw materials using this kind of production method are necessary Subsequent processing program is entered back into after drying.So that the production chain of product is relatively simple.
In this case, it especially wants under the developments such as emphasis Support agriculture (agricultural and sideline product), it is necessary to propose complete New designs by the production of the novel product of Industry Foundation of life science basic theoretical knowledge background.
It is all more fashionable as the technology in forward position using life science, cell biology, but under conventional research method Product development is not embodied.This is exactly that the research of emerging technology serves the one of people's life without preferably exploitation again A embodiment.
Generally speaking, what is relatively lacked at present is the effective exploitation and liter that advanced investigative technique is used for conventional life products Grade.In view of this, deep processing of this patent based on agricultural and sideline product, propose it is a kind of provided with the energy in n cell it is basic The beverage products of substance are wanted based on unit mitochondria.
Summary of the invention
Mitochondria is most studied one of organelle, and conventional separate mode is two step centrifugal separations.At present Until, not with the development and application of organelle product as main component.The inventive point of this patent is the separation of mitochondria Technical application forms the production technology that complete mitochondria takes orally beverage into beverage products production and processing.
The raw materials for production that this patent uses are the fresh vegetables of rich in mitochondria, based on fresh leaf vegetables, such as radish, spinach Dish, garden sorrel, cabbage, three-coloured amaranth are added sucrose, make to form the buffer protection system of 0.25mol/L pH7.2 sucrose solution.Then into Row clasmatosis is generally carried out by the way of high-speed homogenization under controlled temperature conditions.With gauze or slightly after raw material is broken Filter device is filtered.
The first time centrifuge separation of mitochondria production: it is separated off sufficiently not broken plant tissue, by above-mentioned filtered fluid Centrifugal treating in the state of 700 × g of progress, duration 10min collect centrifugate.Discard solid content.
Second of centrifuge separation of mitochondria production: the supernatant of centrifuge separation for the first time is collected, 10000 × g shape is carried out Centrifugal treating under state, duration 10min collect solid content to get mitochondria is arrived.The centrifugate of this when can be used as one As beverage products, it is filling to produce to obtain the final product.Necessary seasoning control can be done if necessary.
Mitochondria oral solution is prepared, next the mitochondria obtained to collection is needed to carry out buffering preparation, usually adopted The dissolution of mitochondria is carried out with 0.25mol/L pH7.2 sucrose phosphate sodium buffer.Mitochondria after dissolution can be adopted directly The oral beverage of general mitochondria is formed with sterile production technology is filling.
In view of being prepared into liposomal product, need to be added necessary macromolecule dispersant, sorbierite, beta ring paste Above-mentioned substance can be added in the buffer of dissolution mitochondria in essence, polyethylene glycol, can also be after dissolution individually with solution The mode of supplement is added.
After the ethanol solution of soybean lecithin, vitamin E, cholesterol heat is dissolved, liposome is prepared using injection method, In the buffer for being added to mitochondria under stirring.The oral lipid body beverage of mitochondria is obtained after sterile filling afterwards.
It is taken orally in beverage production process in the mitochondria of this patent, to prepare 1000L calculating, the service condition of each reagent Part following table executes.
Title rank dosage.
Sucrose food grade 100-300mol.
Sodium phosphate analyzes pure 1-40kg.
Sorbierite analyzes pure 5-260kg.
Beta cyclodextrin analyzes pure 5-100kg.
Polyethylene glycol analyzes pure 2-60kg.
Soybean lecithin food grade 20-100kg.
Vitamin E analyzes pure 3-30kg.
The pure 1-30kg of cholesterol analysis.
The pure 30-250L of (anhydrous) ethanol analysis.
The technology income of this patent.
This patent devises the beverage products constituted based on the mitochondria in vegetables (especially leaf vegetables) source, can be fast The energy safeguard level of fast effective supplement human body.Body is directly improved by increasing intracellular respiration mechanism " mitochondria " Energy safeguard it is horizontal, the effective energy supply for increasing body.
As the beverage products of body energy supplement class, the mitochondria beverage products of this Specialty Design development are to drink in the market Expect the useful supplement of product, while providing a completely new technical solution for the deep processing of agricultural and sideline product.
Detailed description of the invention
Fig. 1 is the process principle figure of vegetables mitochondria oral emulsion of this patent and preparation method thereof.
Specific embodiment
Embodiment 1: the centrifuge separation and the production of general beverage products of mitochondria.
1, raw materials for production: the fresh vegetables of rich in mitochondria, such as radish, spinach, garden sorrel, cabbage, three-coloured amaranth.
2, fresh raw materials for production are cleaned and is sorted, take out damaged rotten part.
3, sucrose and a small amount of water are added according to formula ratio, it is ensured that the intermediate product solution being homogenized forms 0.25mol/L The buffer protection system of pH7.2 sucrose solution.
4, clasmatosis is generally carried out by the way of high-speed homogenization under controlled temperature conditions.Control homogenate is broken The temperature of process is 2-30 DEG C.It is filtered after raw material is broken with gauze or coarse filter device.
5, the first time centrifuge separation of mitochondria: being separated off sufficiently broken plant tissue, by above-mentioned filtered fluid into Centrifugal treating in the state of 700 × g of row, duration 10min collect centrifugate.Discard solid content.
6, second of centrifuge separation of mitochondria separation: the supernatant of centrifuge separation for the first time is collected, 10000 × g is carried out Centrifugal treating under state, duration 10min collect solid content to get mitochondria is arrived.
The centrifugate of this when can be used as general beverage products, filling to produce to obtain the final product.
Embodiment 2: the preparation of mitochondria oral solution.
1, the sediment that centrifugation obtains, as mitochondria raw material are collected.
2, above-mentioned mitochondria is added to 0.25mol/LpH7.2 sucrose phosphate sodium buffer, stirring and dissolving.
3, it with 0.25mol/LpH7.2 sucrose phosphate sodium buffer constant volume, is filled with aseptic filtration technology, aseptic filling technology Dress sealing obtains mitochondria oral solution.
Embodiment 3: the preparation of mitochondria liposome oral fluid.
1, the ethanol solution of phosphatide is prepared.
Soybean lecithin, vitamin E, cholesterol are weighed according to formula ratio, is dissolved, is obtained transparent with (anhydrous) ethyl alcohol of heat Building is to ethanol solution.
2, the allotment of mitochondria buffer.
Sorbierite, beta cyclodextrin, polyethylene glycol are added in the mitochondria lysate of embodiment 2, stirring makes thoroughly Dissolution.
3, injection method prepares liposome.
Maintaining mitochondria buffer temperature is 35-70 DEG C, and under stirring, phosphatide ethanol solution is at the uniform velocity injected into line In plastochondria buffer.Injection finishes, and continues stirring 10-30 minutes.
4, it with 0.25mol/LpH7.2 sucrose phosphate sodium buffer constant volume, is filled with aseptic filtration technology, aseptic filling technology Dress sealing obtains mitochondria lipidosome oral milk beverage.

Claims (6)

1. a kind of oral emulsion (beverage) production technology rich in mitochondria, the technology is using the vegetables rich in mitochondria as raw material, warp Clasmatosis and centrifuge separation mitochondria after, oral emulsion is prepared into using food grade phosphatide, make mitochondria obtain enrichment and Protection, to provide the sufficient energy for life entity.
2. pressing claim 1, the raw material for preparing mitochondria oral emulsion is fresh vegetable raw-material, common easy usually in life In the fresh vegetables class of processing, preferably leaf vegetables raw material, include: radish, spinach, garden sorrel, cabbage, three-coloured amaranth.
3. pressing claim 1, mitochondria, which takes orally milk beverage, can be simple mitochondria beverage, be also possible to encapsulate using phosphatide The lipidosome oral emulsion formed later is the beverage products produced using asptic technique because being related to vital principle.
4. pressing claim 3, Pure mitochondria beverage is to carry out line grain using 0.25mol/L pH7.2 sucrose phosphate sodium buffer The beverage products directly produced with aseptic filling technology after the dissolution of body.
5. pressing claim 3, mitochondria lipidosome oral milk beverage is after using injection method to prepare liposome with phosphatide Encapsulate the beverage products formed.
6. pressing claim 5, the formulation dosage range of mitochondria lipidosome oral milk beverage are as follows: sorbierite 5-260, beta ring paste Smart 5-100, polyethylene glycol 2-60, soybean lecithin 20-100, vitamin E 3-30, cholesterol 1-30.
CN201710449222.5A 2017-06-14 2017-06-14 Vegetables mitochondria oral emulsion and preparation method thereof Pending CN109077199A (en)

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CN109077199A true CN109077199A (en) 2018-12-25

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Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20110123651A1 (en) * 2009-05-20 2011-05-26 Mower Thomas E Dietary supplement drink for delivery of resveratrol and other polyphenols
CN102936592A (en) * 2012-12-06 2013-02-20 四川农业大学 Fast and high-efficiency extracting and purifying method of corn mitochondrial DNA (deoxyribonucleic acid)
CN103181936A (en) * 2011-12-30 2013-07-03 孟泉科 Ginkgo flavone liposome oral emulsion formula and preparation method thereof
CN103239563A (en) * 2013-05-23 2013-08-14 东方中科生命科学有限责任公司 Production method of healthy product capable of enhancing cell life vitality of human body and preparation of method
CN103719968A (en) * 2013-12-10 2014-04-16 马鞍山市安康菌业有限公司 Red ganoderma lucidum health beverage and preparation method thereof

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20110123651A1 (en) * 2009-05-20 2011-05-26 Mower Thomas E Dietary supplement drink for delivery of resveratrol and other polyphenols
CN103181936A (en) * 2011-12-30 2013-07-03 孟泉科 Ginkgo flavone liposome oral emulsion formula and preparation method thereof
CN102936592A (en) * 2012-12-06 2013-02-20 四川农业大学 Fast and high-efficiency extracting and purifying method of corn mitochondrial DNA (deoxyribonucleic acid)
CN103239563A (en) * 2013-05-23 2013-08-14 东方中科生命科学有限责任公司 Production method of healthy product capable of enhancing cell life vitality of human body and preparation of method
CN103719968A (en) * 2013-12-10 2014-04-16 马鞍山市安康菌业有限公司 Red ganoderma lucidum health beverage and preparation method thereof

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Application publication date: 20181225