CN109077199A - Vegetables mitochondria oral emulsion and preparation method thereof - Google Patents
Vegetables mitochondria oral emulsion and preparation method thereof Download PDFInfo
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- CN109077199A CN109077199A CN201710449222.5A CN201710449222A CN109077199A CN 109077199 A CN109077199 A CN 109077199A CN 201710449222 A CN201710449222 A CN 201710449222A CN 109077199 A CN109077199 A CN 109077199A
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- 210000003470 mitochondria Anatomy 0.000 title claims abstract description 56
- 235000013311 vegetables Nutrition 0.000 title claims abstract description 10
- 239000000839 emulsion Substances 0.000 title claims abstract description 8
- 238000002360 preparation method Methods 0.000 title description 6
- 238000004945 emulsification Methods 0.000 title description 2
- 238000004519 manufacturing process Methods 0.000 claims abstract description 16
- 238000005516 engineering process Methods 0.000 claims abstract description 15
- 239000002994 raw material Substances 0.000 claims abstract description 12
- 238000000034 method Methods 0.000 claims abstract description 10
- 239000002502 liposome Substances 0.000 claims abstract description 5
- 235000013361 beverage Nutrition 0.000 claims description 21
- 238000000926 separation method Methods 0.000 claims description 11
- GVJHHUAWPYXKBD-UHFFFAOYSA-N (±)-α-Tocopherol Chemical compound OC1=C(C)C(C)=C2OC(CCCC(C)CCCC(C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-UHFFFAOYSA-N 0.000 claims description 8
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 claims description 8
- 238000004090 dissolution Methods 0.000 claims description 6
- 238000012545 processing Methods 0.000 claims description 6
- DVKFVGVMPLXLKC-PUGXJXRHSA-N [(2s,3r,4s,5s,6r)-2-[(2s,3s,4s,5r)-3,4-dihydroxy-2,5-bis(hydroxymethyl)oxolan-2-yl]-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl] dihydrogen phosphate Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@]1(CO)[C@@]1(OP(O)(O)=O)[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 DVKFVGVMPLXLKC-PUGXJXRHSA-N 0.000 claims description 5
- 239000011734 sodium Substances 0.000 claims description 5
- 229910052708 sodium Inorganic materials 0.000 claims description 5
- JLPULHDHAOZNQI-ZTIMHPMXSA-N 1-hexadecanoyl-2-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phosphocholine Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCC[N+](C)(C)C)OC(=O)CCCCCCC\C=C/C\C=C/CCCCC JLPULHDHAOZNQI-ZTIMHPMXSA-N 0.000 claims description 4
- 239000002202 Polyethylene glycol Substances 0.000 claims description 4
- 229930003427 Vitamin E Natural products 0.000 claims description 4
- 235000012000 cholesterol Nutrition 0.000 claims description 4
- WIGCFUFOHFEKBI-UHFFFAOYSA-N gamma-tocopherol Natural products CC(C)CCCC(C)CCCC(C)CCCC1CCC2C(C)C(O)C(C)C(C)C2O1 WIGCFUFOHFEKBI-UHFFFAOYSA-N 0.000 claims description 4
- 238000002347 injection Methods 0.000 claims description 4
- 239000007924 injection Substances 0.000 claims description 4
- 235000020124 milk-based beverage Nutrition 0.000 claims description 4
- 229920001223 polyethylene glycol Polymers 0.000 claims description 4
- 229940083466 soybean lecithin Drugs 0.000 claims description 4
- 235000019165 vitamin E Nutrition 0.000 claims description 4
- 229940046009 vitamin E Drugs 0.000 claims description 4
- 239000011709 vitamin E Substances 0.000 claims description 4
- 240000001592 Amaranthus caudatus Species 0.000 claims description 3
- 235000009328 Amaranthus caudatus Nutrition 0.000 claims description 3
- 238000012371 Aseptic Filling Methods 0.000 claims description 3
- 240000007124 Brassica oleracea Species 0.000 claims description 3
- 235000003899 Brassica oleracea var acephala Nutrition 0.000 claims description 3
- 235000011301 Brassica oleracea var capitata Nutrition 0.000 claims description 3
- 235000001169 Brassica oleracea var oleracea Nutrition 0.000 claims description 3
- 244000088415 Raphanus sativus Species 0.000 claims description 3
- 235000006140 Raphanus sativus var sativus Nutrition 0.000 claims description 3
- 244000137827 Rumex acetosa Species 0.000 claims description 3
- 235000015807 Rumex acetosa ssp. acetosa Nutrition 0.000 claims description 3
- 235000015447 Rumex acetosa ssp. alpestris Nutrition 0.000 claims description 3
- 235000015448 Rumex acetosa ssp. ambiguus Nutrition 0.000 claims description 3
- 235000015437 Rumex acetosa ssp. arifolius Nutrition 0.000 claims description 3
- 235000015436 Rumex acetosa ssp. lapponicus Nutrition 0.000 claims description 3
- 235000015441 Rumex acetosa ssp. pseudoxyria Nutrition 0.000 claims description 3
- 235000015439 Rumex acetosa ssp. thyrsiflorus Nutrition 0.000 claims description 3
- 244000300264 Spinacia oleracea Species 0.000 claims description 3
- 235000009337 Spinacia oleracea Nutrition 0.000 claims description 3
- 235000012735 amaranth Nutrition 0.000 claims description 3
- 239000004178 amaranth Substances 0.000 claims description 3
- 235000013305 food Nutrition 0.000 claims description 3
- 235000021384 green leafy vegetables Nutrition 0.000 claims description 3
- 238000003825 pressing Methods 0.000 claims 5
- 238000009472 formulation Methods 0.000 claims 1
- 239000000203 mixture Substances 0.000 claims 1
- 229930006000 Sucrose Natural products 0.000 abstract description 6
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 abstract description 6
- 239000005720 sucrose Substances 0.000 abstract description 6
- 230000003834 intracellular effect Effects 0.000 abstract description 3
- 210000001519 tissue Anatomy 0.000 abstract description 3
- 238000001085 differential centrifugation Methods 0.000 abstract 1
- 230000004060 metabolic process Effects 0.000 abstract 1
- 239000008267 milk Substances 0.000 abstract 1
- 210000004080 milk Anatomy 0.000 abstract 1
- 235000013336 milk Nutrition 0.000 abstract 1
- 230000001502 supplementing effect Effects 0.000 abstract 1
- 239000000047 product Substances 0.000 description 21
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 17
- 239000000243 solution Substances 0.000 description 9
- 239000013589 supplement Substances 0.000 description 8
- 235000019441 ethanol Nutrition 0.000 description 6
- 238000003756 stirring Methods 0.000 description 5
- 239000007787 solid Substances 0.000 description 4
- 238000011161 development Methods 0.000 description 3
- 230000018109 developmental process Effects 0.000 description 3
- 238000011049 filling Methods 0.000 description 3
- 239000012530 fluid Substances 0.000 description 3
- 229940100688 oral solution Drugs 0.000 description 3
- 210000003463 organelle Anatomy 0.000 description 3
- 229920000858 Cyclodextrin Polymers 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 2
- 239000001116 FEMA 4028 Substances 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- WHGYBXFWUBPSRW-FOUAGVGXSA-N beta-cyclodextrin Chemical compound OC[C@H]([C@H]([C@@H]([C@H]1O)O)O[C@H]2O[C@@H]([C@@H](O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O3)[C@H](O)[C@H]2O)CO)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@@H]3O[C@@H]1CO WHGYBXFWUBPSRW-FOUAGVGXSA-N 0.000 description 2
- 235000011175 beta-cyclodextrine Nutrition 0.000 description 2
- 229960004853 betadex Drugs 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- 229940107161 cholesterol Drugs 0.000 description 2
- 238000013461 design Methods 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- 239000012467 final product Substances 0.000 description 2
- 238000000265 homogenisation Methods 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 238000007789 sealing Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- 238000012356 Product development Methods 0.000 description 1
- 230000003139 buffering effect Effects 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000002270 dispersing agent Substances 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 235000011194 food seasoning agent Nutrition 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 239000013067 intermediate product Substances 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 239000006166 lysate Substances 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 230000029058 respiratory gaseous exchange Effects 0.000 description 1
- 239000013049 sediment Substances 0.000 description 1
- 239000001488 sodium phosphate Substances 0.000 description 1
- 229910000162 sodium phosphate Inorganic materials 0.000 description 1
- 238000012859 sterile filling Methods 0.000 description 1
- 235000013616 tea Nutrition 0.000 description 1
- RYFMWSXOAZQYPI-UHFFFAOYSA-K trisodium phosphate Chemical compound [Na+].[Na+].[Na+].[O-]P([O-])([O-])=O RYFMWSXOAZQYPI-UHFFFAOYSA-K 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/02—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation containing fruit or vegetable juices
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/52—Adding ingredients
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/52—Adding ingredients
- A23L2/60—Sweeteners
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/115—Fatty acids or derivatives thereof; Fats or oils
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/125—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives containing carbohydrate syrups; containing sugars; containing sugar alcohols; containing starch hydrolysates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/15—Vitamins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Nutrition Science (AREA)
- Mycology (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Molecular Biology (AREA)
- Botany (AREA)
- Non-Alcoholic Beverages (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The present invention provides a kind of using vegetables as the oral emulsion production technology rich in mitochondria of raw material.Mitochondria is intracellular energy generation mechanism, by supplementing mitochondria, can generate more energy for body, strengthen the metabolism of tissue.Then the present invention is aided with liposome preparatory techniques by sucrose differential centrifugation separate mitochondria, to isolated one layer of bimolecular film protection of mitochondria, form the oral milk product of pure taste.
Description
Technical field
This patent provides a kind of processing of agriculture product technology, based on the deep processing of agricultural and sideline product, with modern industry
Production technology combines, and provides a kind of body energy supplement beverage based on organelle " mitochondria ".
Background technique
Exercise makes one strong, and moves and the energy is needed to guarantee, and mitochondria is to provide the pass of the energy safeguard of vital movement
Key organelle.Supplement mitochondria is the shortcut of effective offer life entity energy safeguard.It is reported that relatively being done more physical exercises it
Afterwards, intracellular mitochondria is than having apparent increase in the state of peace and quiet.For from another level, pass through supplementary cords grain
Body is exactly the reliable energy provision level of the raising body of real meaning.
The product of country's beverage market is mainly the supplement beverage of tea beverage and general micro-element or minerals at present, is led to
Replenishing vitamins are crossed to supplement the nutrition of human body.Correspondingly, a large amount of agricultural and sideline product raw materials using this kind of production method are necessary
Subsequent processing program is entered back into after drying.So that the production chain of product is relatively simple.
In this case, it especially wants under the developments such as emphasis Support agriculture (agricultural and sideline product), it is necessary to propose complete
New designs by the production of the novel product of Industry Foundation of life science basic theoretical knowledge background.
It is all more fashionable as the technology in forward position using life science, cell biology, but under conventional research method
Product development is not embodied.This is exactly that the research of emerging technology serves the one of people's life without preferably exploitation again
A embodiment.
Generally speaking, what is relatively lacked at present is the effective exploitation and liter that advanced investigative technique is used for conventional life products
Grade.In view of this, deep processing of this patent based on agricultural and sideline product, propose it is a kind of provided with the energy in n cell it is basic
The beverage products of substance are wanted based on unit mitochondria.
Summary of the invention
Mitochondria is most studied one of organelle, and conventional separate mode is two step centrifugal separations.At present
Until, not with the development and application of organelle product as main component.The inventive point of this patent is the separation of mitochondria
Technical application forms the production technology that complete mitochondria takes orally beverage into beverage products production and processing.
The raw materials for production that this patent uses are the fresh vegetables of rich in mitochondria, based on fresh leaf vegetables, such as radish, spinach
Dish, garden sorrel, cabbage, three-coloured amaranth are added sucrose, make to form the buffer protection system of 0.25mol/L pH7.2 sucrose solution.Then into
Row clasmatosis is generally carried out by the way of high-speed homogenization under controlled temperature conditions.With gauze or slightly after raw material is broken
Filter device is filtered.
The first time centrifuge separation of mitochondria production: it is separated off sufficiently not broken plant tissue, by above-mentioned filtered fluid
Centrifugal treating in the state of 700 × g of progress, duration 10min collect centrifugate.Discard solid content.
Second of centrifuge separation of mitochondria production: the supernatant of centrifuge separation for the first time is collected, 10000 × g shape is carried out
Centrifugal treating under state, duration 10min collect solid content to get mitochondria is arrived.The centrifugate of this when can be used as one
As beverage products, it is filling to produce to obtain the final product.Necessary seasoning control can be done if necessary.
Mitochondria oral solution is prepared, next the mitochondria obtained to collection is needed to carry out buffering preparation, usually adopted
The dissolution of mitochondria is carried out with 0.25mol/L pH7.2 sucrose phosphate sodium buffer.Mitochondria after dissolution can be adopted directly
The oral beverage of general mitochondria is formed with sterile production technology is filling.
In view of being prepared into liposomal product, need to be added necessary macromolecule dispersant, sorbierite, beta ring paste
Above-mentioned substance can be added in the buffer of dissolution mitochondria in essence, polyethylene glycol, can also be after dissolution individually with solution
The mode of supplement is added.
After the ethanol solution of soybean lecithin, vitamin E, cholesterol heat is dissolved, liposome is prepared using injection method,
In the buffer for being added to mitochondria under stirring.The oral lipid body beverage of mitochondria is obtained after sterile filling afterwards.
It is taken orally in beverage production process in the mitochondria of this patent, to prepare 1000L calculating, the service condition of each reagent
Part following table executes.
Title rank dosage.
Sucrose food grade 100-300mol.
Sodium phosphate analyzes pure 1-40kg.
Sorbierite analyzes pure 5-260kg.
Beta cyclodextrin analyzes pure 5-100kg.
Polyethylene glycol analyzes pure 2-60kg.
Soybean lecithin food grade 20-100kg.
Vitamin E analyzes pure 3-30kg.
The pure 1-30kg of cholesterol analysis.
The pure 30-250L of (anhydrous) ethanol analysis.
The technology income of this patent.
This patent devises the beverage products constituted based on the mitochondria in vegetables (especially leaf vegetables) source, can be fast
The energy safeguard level of fast effective supplement human body.Body is directly improved by increasing intracellular respiration mechanism " mitochondria "
Energy safeguard it is horizontal, the effective energy supply for increasing body.
As the beverage products of body energy supplement class, the mitochondria beverage products of this Specialty Design development are to drink in the market
Expect the useful supplement of product, while providing a completely new technical solution for the deep processing of agricultural and sideline product.
Detailed description of the invention
Fig. 1 is the process principle figure of vegetables mitochondria oral emulsion of this patent and preparation method thereof.
Specific embodiment
Embodiment 1: the centrifuge separation and the production of general beverage products of mitochondria.
1, raw materials for production: the fresh vegetables of rich in mitochondria, such as radish, spinach, garden sorrel, cabbage, three-coloured amaranth.
2, fresh raw materials for production are cleaned and is sorted, take out damaged rotten part.
3, sucrose and a small amount of water are added according to formula ratio, it is ensured that the intermediate product solution being homogenized forms 0.25mol/L
The buffer protection system of pH7.2 sucrose solution.
4, clasmatosis is generally carried out by the way of high-speed homogenization under controlled temperature conditions.Control homogenate is broken
The temperature of process is 2-30 DEG C.It is filtered after raw material is broken with gauze or coarse filter device.
5, the first time centrifuge separation of mitochondria: being separated off sufficiently broken plant tissue, by above-mentioned filtered fluid into
Centrifugal treating in the state of 700 × g of row, duration 10min collect centrifugate.Discard solid content.
6, second of centrifuge separation of mitochondria separation: the supernatant of centrifuge separation for the first time is collected, 10000 × g is carried out
Centrifugal treating under state, duration 10min collect solid content to get mitochondria is arrived.
The centrifugate of this when can be used as general beverage products, filling to produce to obtain the final product.
Embodiment 2: the preparation of mitochondria oral solution.
1, the sediment that centrifugation obtains, as mitochondria raw material are collected.
2, above-mentioned mitochondria is added to 0.25mol/LpH7.2 sucrose phosphate sodium buffer, stirring and dissolving.
3, it with 0.25mol/LpH7.2 sucrose phosphate sodium buffer constant volume, is filled with aseptic filtration technology, aseptic filling technology
Dress sealing obtains mitochondria oral solution.
Embodiment 3: the preparation of mitochondria liposome oral fluid.
1, the ethanol solution of phosphatide is prepared.
Soybean lecithin, vitamin E, cholesterol are weighed according to formula ratio, is dissolved, is obtained transparent with (anhydrous) ethyl alcohol of heat
Building is to ethanol solution.
2, the allotment of mitochondria buffer.
Sorbierite, beta cyclodextrin, polyethylene glycol are added in the mitochondria lysate of embodiment 2, stirring makes thoroughly
Dissolution.
3, injection method prepares liposome.
Maintaining mitochondria buffer temperature is 35-70 DEG C, and under stirring, phosphatide ethanol solution is at the uniform velocity injected into line
In plastochondria buffer.Injection finishes, and continues stirring 10-30 minutes.
4, it with 0.25mol/LpH7.2 sucrose phosphate sodium buffer constant volume, is filled with aseptic filtration technology, aseptic filling technology
Dress sealing obtains mitochondria lipidosome oral milk beverage.
Claims (6)
1. a kind of oral emulsion (beverage) production technology rich in mitochondria, the technology is using the vegetables rich in mitochondria as raw material, warp
Clasmatosis and centrifuge separation mitochondria after, oral emulsion is prepared into using food grade phosphatide, make mitochondria obtain enrichment and
Protection, to provide the sufficient energy for life entity.
2. pressing claim 1, the raw material for preparing mitochondria oral emulsion is fresh vegetable raw-material, common easy usually in life
In the fresh vegetables class of processing, preferably leaf vegetables raw material, include: radish, spinach, garden sorrel, cabbage, three-coloured amaranth.
3. pressing claim 1, mitochondria, which takes orally milk beverage, can be simple mitochondria beverage, be also possible to encapsulate using phosphatide
The lipidosome oral emulsion formed later is the beverage products produced using asptic technique because being related to vital principle.
4. pressing claim 3, Pure mitochondria beverage is to carry out line grain using 0.25mol/L pH7.2 sucrose phosphate sodium buffer
The beverage products directly produced with aseptic filling technology after the dissolution of body.
5. pressing claim 3, mitochondria lipidosome oral milk beverage is after using injection method to prepare liposome with phosphatide
Encapsulate the beverage products formed.
6. pressing claim 5, the formulation dosage range of mitochondria lipidosome oral milk beverage are as follows: sorbierite 5-260, beta ring paste
Smart 5-100, polyethylene glycol 2-60, soybean lecithin 20-100, vitamin E 3-30, cholesterol 1-30.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710449222.5A CN109077199A (en) | 2017-06-14 | 2017-06-14 | Vegetables mitochondria oral emulsion and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710449222.5A CN109077199A (en) | 2017-06-14 | 2017-06-14 | Vegetables mitochondria oral emulsion and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109077199A true CN109077199A (en) | 2018-12-25 |
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| CN201710449222.5A Pending CN109077199A (en) | 2017-06-14 | 2017-06-14 | Vegetables mitochondria oral emulsion and preparation method thereof |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20110123651A1 (en) * | 2009-05-20 | 2011-05-26 | Mower Thomas E | Dietary supplement drink for delivery of resveratrol and other polyphenols |
| CN102936592A (en) * | 2012-12-06 | 2013-02-20 | 四川农业大学 | Fast and high-efficiency extracting and purifying method of corn mitochondrial DNA (deoxyribonucleic acid) |
| CN103181936A (en) * | 2011-12-30 | 2013-07-03 | 孟泉科 | Ginkgo flavone liposome oral emulsion formula and preparation method thereof |
| CN103239563A (en) * | 2013-05-23 | 2013-08-14 | 东方中科生命科学有限责任公司 | Production method of healthy product capable of enhancing cell life vitality of human body and preparation of method |
| CN103719968A (en) * | 2013-12-10 | 2014-04-16 | 马鞍山市安康菌业有限公司 | Red ganoderma lucidum health beverage and preparation method thereof |
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Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20110123651A1 (en) * | 2009-05-20 | 2011-05-26 | Mower Thomas E | Dietary supplement drink for delivery of resveratrol and other polyphenols |
| CN103181936A (en) * | 2011-12-30 | 2013-07-03 | 孟泉科 | Ginkgo flavone liposome oral emulsion formula and preparation method thereof |
| CN102936592A (en) * | 2012-12-06 | 2013-02-20 | 四川农业大学 | Fast and high-efficiency extracting and purifying method of corn mitochondrial DNA (deoxyribonucleic acid) |
| CN103239563A (en) * | 2013-05-23 | 2013-08-14 | 东方中科生命科学有限责任公司 | Production method of healthy product capable of enhancing cell life vitality of human body and preparation of method |
| CN103719968A (en) * | 2013-12-10 | 2014-04-16 | 马鞍山市安康菌业有限公司 | Red ganoderma lucidum health beverage and preparation method thereof |
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Application publication date: 20181225 |