CN109077193A - It splits pot algae and its preparation and is improving the application in laying rate of laying hen - Google Patents
It splits pot algae and its preparation and is improving the application in laying rate of laying hen Download PDFInfo
- Publication number
- CN109077193A CN109077193A CN201810300872.8A CN201810300872A CN109077193A CN 109077193 A CN109077193 A CN 109077193A CN 201810300872 A CN201810300872 A CN 201810300872A CN 109077193 A CN109077193 A CN 109077193A
- Authority
- CN
- China
- Prior art keywords
- pot algae
- preparation
- split
- powder
- application
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/70—Feeding-stuffs specially adapted for particular animals for birds
- A23K50/75—Feeding-stuffs specially adapted for particular animals for birds for poultry
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/10—Feeding-stuffs specially adapted for particular animals for ruminants
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/12—Unicellular algae; Culture media therefor
- C12N1/125—Unicellular algae isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/89—Algae ; Processes using algae
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Polymers & Plastics (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Microbiology (AREA)
- Health & Medical Sciences (AREA)
- Food Science & Technology (AREA)
- Animal Husbandry (AREA)
- Birds (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Cell Biology (AREA)
- Biochemistry (AREA)
- Genetics & Genomics (AREA)
- Tropical Medicine & Parasitology (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- Botany (AREA)
- Virology (AREA)
- Molecular Biology (AREA)
- Physiology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention discloses split pot algae and its preparation to improve the application in laying rate of laying hen.In the present invention split pot algae China Committee for Culture Collection of Microorganisms's common micro-organisms center deposit number be CGMCC No.13746.Using it is of the invention split the preparation of pot algae split pot algae powder, the egg laying performance of poultry can be improved, and this hatching egg, organic, safe and stable, easy absorption, it can be used as the safer and more effective approach that people absorb natural DHA, consumer demand can be more catered to and be met, and then of the invention splitting pot algae and split pot algae powder is made to be with a wide range of applications.
Description
Technical field
The present invention relates in field of biotechnology, split pot algae and its preparation to improve the application in laying rate of laying hen.
Background technique
DHA (docosahexaenoic acid) belongs to the serial polyunsaturated fatty acid (ω -3PUFAs) of ω -3, is human cell membrane
With the important composition substance of nerve fiber, there is the important physiology such as brain tonic and intelligence development, the development for promoting optic nerve, prevention and treatment senile dementia
Function is promoting infant growth, is preventing cardiovascular disease, inhibiting and treat certain cancers, guarantees that nervous system is normal
Work etc. plays an important role.
With the raising of living standard and the level of consumption and deepening continuously for DHA physiological function research, for different people
Group DHA intake problem more and more attention has been paid to." Chinese DHA eats condition survey " completed according to Chinese food association
It has been shown that, China is public, and direct DHA intake is only about 40mg from food daily, in serious " starvation " state of DHA.From diet
Middle acquisition DHA has become the common recognition of people, wherein, the dairies such as milk, goat milk rich in high-quality DHA especially prevalent with dairy products
The annual demand of product constantly rises, and high-quality DHA is absorbed from dairy produce, has become a kind of trend.
DHA content in ordinary milk is extremely low, it is difficult to meet people's daily demand, and dairy products external source addition DHA is then deposited
It is more in material requested, larger consumption enterprise's production cost, adding procedure easily cause DHA price reduction, decompose or generate peculiar smell the problems such as.
By suitably increasing the polyunsaturated fatty acids intake such as DHA in ruminant, milk can be improved, in musculature
DHA content, but the special digestion structure of ruminant converts the polyunsaturated fatty acids such as DHA largely after entering cud
At saturated fatty acid, the polyunsaturated fatty acids utilization rate such as DHA is greatly reduced.The rumen-protected technology of fatty acid on the market
Mainly there are coating, hydrogenation, calcification etc., not only processing technique difficulty is big, and the production cost is very high, and there are daily feedings to reduce,
Digestibility decline, the defects of generating butterfat drop syndrome, or even cause toxic side effect.Therefore, it is dynamic how to improve lactation
In object especially ruminant milk the content of DHA and further using ruminant body conversion metaplasia produce be rich in DHA days
Right organic milk is current urgent problem.
In addition, can not only enrich the source DHA by the content for improving DHA in egg, also have greatly expanded answering for DHA
With field and sphere of consumption.
Summary of the invention
The technical problem to be solved by the present invention is to how improve animal product yield.
In order to solve the above technical problems, present invention firstly provides split pot algae (Schizochytrium limacinum) or
Following any applications of its preparation:
B1) application in animal product yield is being improved；
B2) the application in the substance that preparation improves animal product yield.
It is described that split pot algae (Schizochytrium limacinum) can be to split pot algae (Schizochytrium
Limacinum) HS01, described pot algae (Schizochytrium limacinum) HS01 that splits is in Chinese microorganism strain preservation pipe
The deposit number of reason committee common micro-organisms center is CGMCC No.13746.
The active constituent of the preparation can split pot algae (Schizochytrium limacinum) to be described.
The animal can be poultry.The animal concretely chicken.The chicken be further Beijing Cold boiled chicken, the blue Cold boiled chicken in sea,
The blue powder shell chicken of extra large blue brown laying hen or sea.
The animal product is the egg that the animal produces.
The preparation can be to split pot algae powder.
The preparation can be according to the method preparation method of pot algae preparation (the be denoted as this method split) system included the following steps
It is standby: to split pot algae (Schizochytrium limacinum) described in culture, obtain fermentation liquid；It is prepared using the fermentation liquid
The preparation.
Split in above-mentioned application, described in culture pot algae (Schizochytrium limacinum) using fermentation medium into
Row, the fermentation medium are made of solvent and solute, and the solvent is water, and the solute and its concentration are respectively glucose 60
~150g/L, 8~25g/L of yeast extract, 3~8g/L of yeast powder, Na2SO45~20g/L, 0.5~1.5g/L of KCl, MgSO4
1.0~3.0g/L, K2SO40.5~2.5g/L, KH2PO41.0~2.0g/L, (NH4)2SO42.0~5.0g/L, CaCl2 0.5
~2.5g/L, CuSO40.001~0.02g/L, ZnSO40.001~0.02g/L, 0.001~0.06g/L of biotin, starch
0.1~10g/L and albumen powder 0~20g/L, pH are 4.5~6.5.
The starch can be cornstarch or starch Sodium Octenyl Succinate, and the albumen powder can be Fed Protein Powder of Pea Insteal or cream
Albumin powder.The pH of the fermentation medium concretely 6.
The solute and its concentration of the fermentation medium concretely following n1) or n2) or n3) or n4):
N1) glucose 60g/L, yeast extract 8g/L, yeast powder 3g/L, Na2SO4 5g/L、KCl 0.5g/L、MgSO4
1.0g/L、K2SO4 0.5g/L、KH2PO4 1.0g/L、(NH4)2SO4 2.0g/L、CaCl2 0.5g/L、CuSO4 0.001g/L、
ZnSO40.001g/L, biotin 0.001g/L and cornstarch 0.1g/L；
N2) glucose 150g/L, yeast extract 25g/L, yeast powder 8g/L, Na2SO4 20g/L、KCl 1.5g/L、MgSO4
3.0g/L、K2SO4 2.5g/L、KH2PO4 2.0g/L、(NH4)2SO4 5.0g/L、CaCl2 2.5g/L、CuSO4 0.02g/L、
ZnSO40.02g/L, biotin 0.06g/L, cornstarch 10g/L and Fed Protein Powder of Pea Insteal 20g/L；
N3) glucose 60g/L, yeast extract 8g/L, yeast powder 3g/L, Na2SO4 5g/L、KCl 0.5g/L、MgSO4
1.0g/L、K2SO4 0.5g/L、KH2PO4 1.0g/L、(NH4)2SO4 2.0g/L、CaCl2 0.5g/L、CuSO4 0.001g/L、
ZnSO40.001g/L, biotin 0.001g/L and starch Sodium Octenyl Succinate 0.1g/L；
N4) glucose 150g/L, yeast extract 25g/L, yeast powder 8g/L, Na2SO4 20g/L、KCl 1.5g/L、MgSO4
3.0g/L、K2SO4 2.5g/L、KH2PO4 2.0g/L、(NH4)2SO4 5.0g/L、CaCl2 2.5g/L、CuSO4 0.02g/L、
ZnSO40.02g/L, biotin 0.06g/L, starch Sodium Octenyl Succinate 10g/L and PURE WHEY 20g/L.
It is described that the preparation is prepared using the fermentation liquid in above-mentioned application can include: the dry fermentation liquid obtains
To the preparation.
The above method may additionally include to carry out again after addition antioxidant after obtaining the fermentation liquid into the fermentation liquid
Pot algae powder is split described in being dried to obtain.
The antioxidant can be the molten antioxidant of oil and/or water soluble antioxidant.The molten antioxidant of oil can be fan
Repeatedly perfume, mixed tocopherols, tea polyphenols and/or ascorbyl palmitate.The water soluble antioxidant can be phytic acid, anti-bad
Hematic acid and/or arabo-ascorbic acid.
The antioxidant several different specific antioxidants when being made of, and proportion no requirement (NR) between each ingredient can
It adjusts according to specific needs.
The antioxidant is concretely by mixed tocopherols, rosemary, tea polyphenols, arabo-ascorbic acid and phytic acid group
At mixing antioxidant.Proportion in the mixing antioxidant between each substance can be following p1), p2), p3) or p4):
P1) mass ratio of mixed tocopherols, rosemary, tea polyphenols, arabo-ascorbic acid and phytic acid is 20:2:10:10:
2；
P2) mass ratio of mixed tocopherols, rosemary, tea polyphenols, arabo-ascorbic acid and phytic acid is 40:3:20:20:
4；
P3) mass ratio of mixed tocopherols, rosemary, tea polyphenols, arabo-ascorbic acid and phytic acid is 60:2:40:30:
6；
P4) mass ratio of mixed tocopherols, rosemary, tea polyphenols, arabo-ascorbic acid and phytic acid is 80:2:40:40:
8。
The drying can be spray drying or roller drying or freeze-drying.
The above method, which may also include, to split pot algae (Schizochytrium limacinum) described in the fermentation liquid
It is washed.The above method further may also include splitting in pot algae (Schizochytrium limacinum) after washing and add
It is dried again after adding the antioxidant.
The dissolved oxygen amount of the culture can be 0~80% (such as 10~80%).The temperature of the culture can be 20~30 DEG C.Institute
The time for stating culture can be 72~120h.
In order to solve the above technical problems, the present invention also provides the methods for improving animal product yield, which comprises
Feeding animals split pot algae (Schizochytrium limacinum) or its preparation is realized and improves the animal product yield.
It is described that split pot algae (Schizochytrium limacinum) can be to split pot algae (Schizochytrium
Limacinum) HS01, described pot algae (Schizochytrium limacinum) HS01 that splits is in Chinese microorganism strain preservation pipe
The deposit number of reason committee common micro-organisms center is CGMCC No.13746.
The active constituent of the preparation can split pot algae (Schizochytrium limacinum) to be described.
In the above method, the animal can be poultry.The animal further can be chicken.The chicken is further that Beijing is white
Chicken, the blue Cold boiled chicken in sea, the blue powder shell chicken of the blue brown laying hen in sea or sea.
In the above method, the animal product can be the egg of animal production.
In the above method, the raising animal product quality can be the raising animal product DHA content and/or reduction institute
State animal product cholesterol level.
In the above method, the preparation can be prepared using the preparation method for splitting pot algae preparation.
Using it is of the invention split pot algae (Schizochytrium limacinum) preparation split pot algae powder, house can be improved
The egg laying performance of fowl, and this hatching egg, organic, safe and stable, easy absorption can be used as people and absorb the safer of natural DHA
Consumer demand can more be catered to and be met to effective way, so make it is of the invention split pot algae and split pot algae powder and have widely answer
With value.
Biomaterial preservation explanation
The classification naming of biomaterial: Schizochytrium limacinum
The strain number of biomaterial: HS01
Depositary institution's title of biomaterial: China Committee for Culture Collection of Microorganisms's common micro-organisms center
Depositary institution's abbreviation of biomaterial: CGMCC
The depositary institution address of biomaterial: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, Chinese Academy of Sciences microorganism
Research institute, postcode: 100101
The preservation date of biomaterial: on March 10th, 2017
The collection of biomaterial is registered on the books number: CGMCC No.13746
Specific embodiment
The present invention is further described in detail With reference to embodiment, and the embodiment provided is only for explaining
The bright present invention, the range being not intended to be limiting of the invention.Experimental method in following embodiments is unless otherwise specified
Conventional method.Material as used in the following examples, reagent, instrument etc., are commercially available unless otherwise specified.
Quantitative test in following embodiment, is respectively provided with three repeated experiments, and results are averaged.
Screening fluid nutrient medium: by glucose 50g and yeast powder 15g be dissolved in 1L mixed liquor (by 1 parts by volume natural sea-water and
1 volume distilled water mixes), pH value is natural.
Screening flat board: about 55 DEG C of screening solid medium is poured into culture dish, the solid plate obtained after cooling.
Fermentation medium: by glucose 60g, glutamic acid or sodium glutamate 10g, Dried Corn Steep Liquor Powder 10g, NaSO4 14g、
KCl 0.5g、MgSO4 2.0g、K2SO4 1.0g、KH2PO4 1.0g、(NH4)2SO41.0g and CaCl20.5g is dissolved in 1L distillation
Water adjusts pH value to 6.0.
Wort agar medium: 150g fructus hordei germinatus leaching powder is dissolved in 1L mixed liquor (by 1 parts by volume natural sea-water and 1 volume
Distilled water mixes), pH value is natural；Then it is 15g/100mL, obtained culture medium that agar powder to its concentration, which is added,.
Mixed tocopherols are ADM Products, article No. MTS-90；Rosemary is that Ke Naiou trade (Shanghai) is limited
Company's Guangzhou Branch product, article No. are ROSEMARY 41-19-58；Tea polyphenols are the source Fujian Li Kang bioengineering Co., Ltd
Product, article No. TP-98；Arabo-ascorbic acid is that ocean experiment Co., Ltd's product is opened up in Zhengzhou, and article No. is content >=98%；Phytic acid is
Laiyang City Wan Jiwei bioengineering Co., Ltd product.
The separation identification of embodiment 1, fragmentation pot HS01
One, the separation of fragmentation pot HS01
1, present inventor acquires fragmentation pot from Zhangzhou City of Fujian Province Yunxiao County mangrove many places, and mixing is mixed
Close liquid；0.5mL mixed liquor is seeded to 5mL screening fluid nutrient medium, then 25 DEG C, 200rpm/min culture 2d are cultivated
Bacterium solution.
2, the culture bacterium solution that step 1 obtains is spread evenly across in screening flat board, 25 DEG C of stationary culture 2d, generates single bacterium
It falls.
3, it after completing step 2, picks them separately single colonie and is seeded to 5mL fermentation medium, then 25 DEG C, 200rpm/min training
2d is supported, culture bacterium solution is obtained.
4, the culture bacterium solution for taking step 3 to obtain, 4 DEG C, 2000rpm centrifugation 5min, collects thallus.
5, take 1.0~2.0g of thallus into tool plug graduated cylinder (specification 100mL), it is 8.3mol/L's that 15mL concentration, which is first added,
HCL aqueous solution covers lid, and being placed in 50~60min of hydrolysis in 70~80 DEG C of water-baths, (period, every 10min was placed in eddy mixer
Upper oscillation tool plug graduated cylinder 1 time)；To be cooled that 10mL 95% (v/v) ethanol water is first added to after room temperature, shake well is uniform
Afterwards, it adds 20mL anhydrous ether shake well and extracts 1~2min, be eventually adding 20mL petroleum ether, shake well extraction 1~
Upper organic phase is placed in glass weighing disk (dried and claimed bare weight) by 2min, stratification, which is placed in logical
Organic phase is set sufficiently to evaporate clean (must sufficiently volatilize clean) on boiling water bath in wind cupboard, liquid phase is grease.
6, the grease for taking step 5 to extract detects DHA content according to GB 26400-2011 national food safety standard, according to
The composition and content of the method detection fatty acid of AOAC996.06.
The higher bacterial strain of DHA content is selected, is purified 24 times repeatedly.Screen one plant of schizochytrium limacinum strain is named as fragmentation
Pot HS01.
Fragmentation pot HS01 monoclonal is seeded to 12 generation of fermentation medium continuous passage and is contained according to above-mentioned steps detection DHA
Amount.The result shows that fragmentation pot HS01 production DHA's has good stability.
Two, the identification of fragmentation pot HS01
1, Morphological Identification
Fragmentation pot HS01 is seeded in wort agar medium, 25 DEG C of dark cultures, observes the form of bacterium colony simultaneously after 5d
Pass through the morphological feature of high resolution TEM analysis and observation thallus.
The result shows that the colony diameter of fragmentation pot HS01 is 2~4.3mm, white (later period light orange), edge is irregular；
Thallus is proliferated in a manner of fragmentation, and cell wall is thin, and spherical, colourless or light orange is transparent, and size is 4.5~15.5 μm, travelling
Spore and expolasm net have no.
2,18s rDNA sequence homology analysis
The partial sequence of the 18s rDNA of fragmentation pot HS01 is as shown in the sequence 1 in sequence table.
The partial sequence of the 18s rDNA of fragmentation pot HS01 is as shown in the sequence 2 in sequence table.
In summary each qualification result, fragmentation pot HS01 are fragmentation pot (Schizoochytrium limacinum).
Three, the preservation of fragmentation pot HS01
Fragmentation pot (Schizoochytrium limacinum) HS01 has been preserved in the micro- life of China on 03 10th, 2017
Object culture presevation administration committee common micro-organisms center (abbreviation CGMCC, address are as follows: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City
No. 3), deposit number is CGMCC No.13746.
Embodiment 2, the preparation for splitting pot algae powder
Using embodiment 1 split pot algae HS01 preparation split pot algae powder the step of it is as follows, experiment in triplicate, be as a result averaged
Value:
One, the preparation of culture medium
Shake flask medium 1 is made of solute and solvent, and solvent is water, and solute and its concentration are respectively 60g/L glucose, ferment
Female cream 5g/L；Shake flask medium 2 is made of solute and solvent, and solvent is water, solute and its concentration be respectively 150g/L glucose,
Yeast extract 25g/L.
Seed culture medium 1 is made of solute and solvent, and solvent is water, and solute and its concentration are respectively glucose 60g/L, ferment
Female cream 8g/L, yeast powder 3g/L, Na2SO4 5g/L、KCl 0.5g/L、MgSO4 1.0g/L、K2SO4 0.5g/L、KH2PO4
1.0g/L、(NH4)2SO4 2.0g/L、CaCl2 0.5g/L、CuSO4 0.001g/L、ZnSO40.001g/L, benefit after the completion of configuration
Initial pH6.0 is adjusted with alkali (sodium hydroxide solution or ammonium hydroxide)；Seed culture medium 2 is made of solute and solvent, and solvent is water,
Solute and its concentration are respectively glucose 150g/L, yeast extract 25g/L, yeast powder 8g/L, Na2SO4 20g/L、KCl 1.5g/L、
MgSO4 3.0g/L、K2SO4 2.5g/L、KH2PO4 2.0g/L、(NH4)2SO4 5.0g/L、CaCl2 2.5g/L、CuSO4
0.02g/L、ZnSO40.02g/L adjusts initial pH6.0 using alkali (sodium hydroxide solution or ammonium hydroxide) after the completion of configuration.
Fermentation medium 1 is made of solute and solvent, and solvent is water, and solute and its concentration are respectively glucose 60g/L, ferment
Female cream 8g/L, yeast powder 3g/L, Na2SO4 5g/L、KCl 0.5g/L、MgSO4 1.0g/L、K2SO4 0.5g/L、KH2PO4
1.0g/L、(NH4)2SO4 2.0g/L、CaCl2 0.5g/L、CuSO4 0.001g/L、ZnSO40.001g/L, biotin
0.001g/L, cornstarch 0.1g/L adjust initial pH6.0 using alkali (sodium hydroxide solution or ammonium hydroxide) after the completion of configuration；
Fermentation medium 2 is made of solute and solvent, and solvent is water, and solute and its concentration are respectively glucose 150g/L, yeast extract
25g/L, yeast powder 8g/L, Na2SO4 20g/L、KCl 1.5g/L、MgSO4 3.0g/L、K2SO4 2.5g/L、KH2PO4 2.0g/
L、(NH4)2SO4 5.0g/L、CaCl2 2.5g/L、CuSO4 0.02g/L、ZnSO40.02g/L, biotin 0.06g/L, corn
Starch 10g/L, Fed Protein Powder of Pea Insteal 20g/L are adjusted initially after the completion of configuration using alkali (sodium hydroxide solution or ammonium hydroxide)
pH6.0；Fermentation medium 3 is made of solute and solvent, and solvent is water, and solute and its concentration are respectively glucose 60g/L, yeast
Cream 8g/L, yeast powder 3g/L, Na2SO4 5g/L、KCl 0.5g/L、MgSO4 1.0g/L、K2SO4 0.5g/L、KH2PO4 1.0g/
L、(NH4)2SO4 2.0g/L、CaCl2 0.5g/L、CuSO4 0.001g/L、ZnSO4It is 0.001g/L, biotin 0.001g/L, pungent
Starch alkenyl succinate sodium 0.1g/L adjusts initial pH6.0 using alkali (sodium hydroxide solution or ammonium hydroxide) after the completion of configuration；Hair
Ferment culture medium 4 is made of solute and solvent, and solvent is water, and solute and its concentration are respectively glucose 150g/L, yeast extract 25g/
L, yeast powder 8g/L, Na2SO4 20g/L、KCl 1.5g/L、MgSO4 3.0g/L、K2SO4 2.5g/L、KH2PO4 2.0g/L、
(NH4)2SO4 5.0g/L、CaCl2 2.5g/L、CuSO4 0.02g/L、ZnSO40.02g/L, biotin 0.06g/L, octenyl
Succinic acid starch sodium 10g/L, PURE WHEY 20g/L are adjusted after the completion of configuration using alkali (sodium hydroxide solution or ammonium hydroxide)
Initial pH6.0.
Two, preparation and the Indexs measure of pot algae powder are split
1, it prepares
Pot algae HS01 will be split to be seeded in Shake flask medium 1, cultivated for 24 hours under conditions of revolving speed 200rpm, 20 DEG C of temperature,
Obtain shake flask culture 1；Shake flask culture 1 is seeded in seed culture medium 1, is that 10~80% (growth course is molten in dissolved oxygen
Solution oxygen is a dynamic process), cultivate 48h under conditions of 20 DEG C of temperature, keep during the cultivation process the pH of culture solution be 4.5~
Between 6.5, fermentation process pH can decline, and be adjusted by ammonium hydroxide or sodium hydroxide solution, obtain seed culture fluid 1；It will
Seed culture fluid 1 is seeded in fermentation medium 1 according to 10% inoculum concentration, in (the growth course dissolved oxygen of dissolved oxygen 10~80%
A dynamic process), cultivate 120h under conditions of 20 DEG C of temperature, obtain fermentation liquid, which be denoted as fermentation liquid 1,
Keeping the pH of culture solution during the cultivation process is between 4.5~6.5, and fermentation process pH can decline, and pass through ammonium hydroxide or hydroxide
Sodium solution is adjusted.
Pot algae HS01 will be split to be seeded in Shake flask medium 2, cultivate 48h under conditions of revolving speed 400rpm, 30 DEG C of temperature,
Obtain shake flask culture 2；Shake flask culture 2 is seeded in seed culture medium 2, is 10~80%, 30 DEG C of temperature in dissolved oxygen
Under the conditions of cultivate for 24 hours, during the cultivation process keep culture solution pH be 4.5~6.5 between, fermentation process pH can decline, and pass through
Ammonium hydroxide or sodium hydroxide solution are adjusted, and obtain seed culture fluid 2；Seed culture fluid 2 is connect according to 20% inoculum concentration
Kind cultivates 72h under conditions of dissolved oxygen 10~80%, 30 DEG C of temperature, fermentation liquid is obtained, by the fermentation into fermentation medium 2
Liquid is denoted as fermentation liquid 2, and keeping the pH of culture solution during the cultivation process is between 4.5~6.5, and fermentation process pH can decline, and pass through
Ammonium hydroxide or sodium hydroxide solution are adjusted.
Pot algae HS01 will be split to be seeded in Shake flask medium 1, cultivated for 24 hours under conditions of revolving speed 200rpm, 20 DEG C of temperature,
Obtain shake flask culture 1；Shake flask culture 1 is seeded in seed culture medium 1, is 10~80%, 20 DEG C of temperature in dissolved oxygen
Under the conditions of cultivate 48h, during the cultivation process keep culture solution pH be 4.5~6.5 between, fermentation process pH can decline, and pass through
Ammonium hydroxide or sodium hydroxide solution are adjusted, and obtain seed culture fluid 1；Seed culture fluid 1 is connect according to 10% inoculum concentration
Kind cultivates 120h under conditions of dissolved oxygen 0~80%, 20 DEG C of temperature, fermentation liquid is obtained, by the fermentation into fermentation medium 3
Liquid is denoted as fermentation liquid 3, and between pH4.5~6.5 for keeping culture solution during the cultivation process, fermentation process pH can decline, and pass through ammonia
Water or sodium hydroxide solution are adjusted.
Pot algae HS01 will be split to be seeded in Shake flask medium 2, cultivated for 24 hours under conditions of revolving speed 400rpm, 30 DEG C of temperature,
Obtain shake flask culture 2；Shake flask culture 2 is seeded in seed culture medium 2, is 10~80%, 30 DEG C of temperature in dissolved oxygen
Under the conditions of cultivate for 24 hours, during the cultivation process keep culture solution pH4.5~6.5 between, fermentation process pH can decline, and pass through ammonia
Water or sodium hydroxide solution are adjusted, and obtain seed culture fluid 2；Seed culture fluid 2 is inoculated with according to 20% inoculum concentration
Into fermentation medium 4,72h is cultivated under conditions of dissolved oxygen 10~80%, 30 DEG C of temperature, fermentation liquid is obtained, by the fermentation liquid
It is denoted as fermentation liquid 4, between pH4.5~6.5 for keeping culture solution during the cultivation process, fermentation process pH can decline, and pass through ammonium hydroxide
Or sodium hydroxide solution is adjusted.
After fermentation, into fermentation liquid 1 add antioxidant 1 (antioxidant 1 by mixed tocopherols, rosemary,
Tea polyphenols, arabo-ascorbic acid and phytic acid composition, wherein mixed tocopherols, rosemary, tea polyphenols, arabo-ascorbic acid and plant
The mass ratio of acid is 20:2:10:10:2) mixed liquor is obtained, it is mixed tocopherols in the mixed liquor, rosemary, tea polyphenols, different
The mass percentage content of ascorbic acid and phytic acid is respectively 0.2%, 0.02%, 0.1%, 0.1% and 0.02%, by the mixing
Liquid is mixed through Over emulsfication, obtains stable fermentation liquid 1；Stable fermentation liquid 1 is subjected to pasteurization, then carries out being sprayed, roll
Cylinder or freeze-drying prepare and split pot algae powder 1.
Antioxidant 2 is added into fermentation liquid 2, and (antioxidant 2 is by mixed tocopherols, rosemary, tea polyphenols, different anti-
Bad hematic acid and phytic acid composition, wherein mixed tocopherols, rosemary, tea polyphenols, arabo-ascorbic acid and phytic acid mass ratio be
Mixed liquor 40:3:20:20:4) is obtained, mixed tocopherols, rosemary, tea polyphenols, arabo-ascorbic acid and plant in the mixed liquor
The mass percentage content of acid is respectively 0.4%, 0.03%, 0.2%, 0.2% and 0.04%, which is mixed through Over emulsfication
It is even, obtain stable fermentation liquid 2；Stable fermentation liquid 2 is subjected to pasteurization, spraying, roller is then carried out or freezing is dry
Dry prepare splits pot algae powder 2.
Fermentation liquid 3 is split into pot frustule mud by being collected by centrifugation, same volume is added according to pot frustule mud volume is split
Aseptic deionized water mixes, and is then centrifuged, repeated washing 2~3 times, and pot frustule mud is split in acquisition；Pot frustule is split to this
Antioxidant 3 is added in mud, and (antioxidant 3 is by mixed tocopherols, rosemary, tea polyphenols, arabo-ascorbic acid and phytic acid group
Mass ratio at, wherein mixed tocopherols, rosemary, tea polyphenols, arabo-ascorbic acid and phytic acid is 60:2:40:30:6)
Mixture is obtained, the quality percentage of mixed tocopherols, rosemary, tea polyphenols, arabo-ascorbic acid and phytic acid in the mixture
It is respectively 0.6%, 0.02%, 0.4%, 0.3% and 0.06% than content, which is mixed through Over emulsfication, is stablized
Cell mud；The stable cell mud is passed through into pasteurization, (spraying, roller or freeze-drying, three is then dried
It selects one) to prepare and splits pot algae powder, this is split pot algae powder and is denoted as and splits pot algae powder 3.
Fermentation liquid 4 is split into pot frustule mud by being collected by centrifugation, same volume is added according to pot frustule mud volume is split
Aseptic deionized water mixes, and is then centrifuged, repeated washing 2~3 times, and pot frustule mud is split in acquisition；Pot frustule is split to this
Antioxidant 4 is added in mud, and (antioxidant 4 is by mixed tocopherols, rosemary, tea polyphenols, arabo-ascorbic acid and phytic acid group
Mass ratio at, wherein mixed tocopherols, rosemary, tea polyphenols, arabo-ascorbic acid and phytic acid is 80:2:40:40:8)
Mixture is obtained, the quality percentage of mixed tocopherols, rosemary, tea polyphenols, arabo-ascorbic acid and phytic acid in the mixture
It is respectively 0.8%, 0.02%, 0.4%, 0.4% and 0.08% than content, which is mixed through Over emulsfication, is stablized
Cell mud；The stable cell mud is passed through into pasteurization, (spraying, roller or freeze-drying, three is then dried
It selects one) to prepare and splits pot algae powder, this is split pot algae powder and is denoted as and splits pot algae powder 4.
2, Indexs measure
What detecting step 1 obtained respectively splits the protein of pot algae powder 1-4, the content of moisture, fatty acid, ash content, DHA, tool
Body detecting method is as follows:
The detection of protein: it is carried out according to GB 5009.9 " measurement of national food safety standard Protein in Food ".
The detection of moisture: it is carried out according to GB 5009.3 " measurement of moisture in national food safety standard food ".
The detection of ash content: it is carried out according to GB 5009.4 " measurement of ash content in national food safety standard food ".
The detection of fatty acid: it is carried out according to GB 5009.168 " fatty acid determination in national food safety standard food ".
The detection of DHA: according to " the national food safety standard food additives docosahexaenoic acid grease of GB 26400
(fermentation method) " it carries out.
The results show that the mass content that protein in pot algae powder is split obtained in above-mentioned steps is 10~60%, moisture
Mass content is 0.5~3.0%, and the mass content of ash content is 3~12%, and the mass content of fatty acid is 25~50%.In rouge
In fat acid, the mass content of unsaturated fatty acid DHA is that the mass content of 10~24%, DPA is the matter of 2.0~6.0%, EPA
Measuring content is 0.1~0.5%.
Table 1, the Indexs measure result for splitting pot algae powder
Note: the content of each index refers to that each substance accounts for the mass percent of dry powder in table 1；Other fatty acid refer to except C8:0
Octanoic acid, C10:0 certain herbaceous plants with big flowers acid, C16:0 palmitinic acid, C18:0 stearic acid, the fatty acid outside C22:5DPA, C20:5EPA and C22:6DHA.
Three, the fistula experiment test of pot algae powder is split
It is tested using the cow rumen fistula of crossing that Nylon Bag split pot algae powder 1 and 2.Its operating procedure is as follows:
1, experimental animal and daily ration
Milk cow (holstein cow) equipped with permanent stomach fistula.Pre-feeding period 7 days, expelling parasite is carried out, during this period with 1%
Metrifonate medical fluid drives away vermin, and the levamisole hydrochloride for taking orally 0.8mg/kg weight drives away endoparasite.In nutrition
Horizontal is to raise under conditions of 1.3 times of maintenances need, and equivalent is fed twice daily, and 07:00 and 16:00 are respectively fed once, after feeding certainly
By drinking water.
2, the preparation of sample
It splits pot algae powder and uses " quartering " random acquisition sample, after 65 DEG C dry to constant weight, it is spare to be packed into port grinding bottle.
3, the production of Nylon Bag
With 300 mesh nylon cloths, it is cut into the rectangle of 170mm × 130mm, terylene linear slit two pass is used after doubling, size is made
For the Nylon Bag of 120mm × 80mm, dissipates side and plated with soldering iron.Nylon Bag is put into cud balance 72h and takes out to clean before test and is dried
Dry, inspection can be used without breakage.
4, experimental design and measuring method
Nylon Bag Rumen is carried out by the scheme of the propositions such as raising dairy cattle standard scientific research cooperative groups, and test is using random
Randomized block design, every ox each time point set two repetitions.
Pot algae powder 10g or so is split in each Nylon Bag loading, and sample variance analysis difference is not significant (P > 0.05).Every 2 bags of jails
It ties up on half polyethylene pipe of a 30cm long, Nylon Bag is placed on cud abdomen capsule portion, the other end of pipe by 2h after raising in morning
Fistula is hung over to cover.6 root canals are put simultaneously in every bovine rumen, totally 12 bags.After putting bag 0h, 2h, 4h, 6h, 12h, for 24 hours,
8 time points of 36h and 48h take out a root canal from the cud of every ox, are cleaned with clear water, are put into rinsing in washing machine 7 and divide
Clock, until clarification of water, then dries to constant weight at 65 DEG C, and weigh.
The measurement of dry matter (DM) is carried out by GB6435-86 method, and the measurement of DHA is by Xiamen Huisheng Biological Co., Ltd.
It is responsible for.Certain nutrient degradability/%=(certain remaining nutritional quality of 1-/at sample time point (t) is put into certain nutrient in bag
Gross mass) × 100%.
The results are shown in Table 2, split as the result is shown pot algae powder cross cow rumen highest degradation rate be 54.7%.
Table 2, the fistula experimental result for splitting pot algae powder
Embodiment 3, embodiment 2 the pot algae powder that splits DHA content in milk can be improved
The present embodiment splits pot by the DHA content split pot algae powder, detect in milk to Cow-feeding embodiment 2, determination
Influence of the algae powder to DHA content in milk, experiment is in triplicate.
One, method for breeding:
Weight, healthy holstein cow of the monthly age without significant difference 60 are chosen, is randomly divided into six groups, every group 10,
That is free-ranging experimental group 1, free-ranging experimental group 2, free-ranging blank control group, stable breeding experimental group 1, stable breeding experimental group 2 and stable breeding blank pair
According to group.
Carrying out pre-feeding period cultivation to each experimental group milk cow first, (pre-feeding period is 15 days, gradually according to the adaptation situation of milk cow
Increasing amount splits pot algae powder feeding volume)；The formal nursing phase later will split pot algae powder according in certain adding proportion investment feed
It is stirred mixing, it is primary every feeding in 8 hours.
The pot algae powder that splits added in the feed of free-ranging experimental group 1 and free-ranging experimental group 2 is respectively that embodiment 1 splits pot algae powder
1 and 2, the pot algae powder that splits added in the feed of stable breeding experimental group 1 and stable breeding experimental group 2 is respectively that embodiment 1 splits 1 He of pot algae powder
2, specific feeding method is as follows:
Pre-feeding period: the amount that pot algae powder is split in experimental group feeding gradually increases, specific as follows:
The scale of feeding that pre-feeding period splits pot algae powder on the the 1st and 2 day is 50mg/ days/head；
The scale of feeding that pre-feeding period splits pot algae powder on the the 3rd and 4 day is 75mg/ days/head；
The scale of feeding that pre-feeding period splits pot algae powder on the the 5th and 6 day is 100mg/ days/head；
The scale of feeding that pre-feeding period splits pot algae powder on the the 7th and 8 day is 125mg/ days/head；
The scale of feeding that pre-feeding period splits pot algae powder on the the 9th and 10 day is 150mg/ days/head；
The scale of feeding that pre-feeding period splits pot algae powder on the the 11st and 12 day is 200mg/ days/head；
The scale of feeding that pre-feeding period splits pot algae powder on the the 13rd, 14 and 15 day is 250mg/ days/head.
The formal nursing phase is to split 250mg/ days/head of powder of pot algae.
It is not added in the feed of blank control group Cow-feeding and splits pot algae powder, remaining ingredient is identical as experimental group, when feeding
Between and the same experimental group of scale of feeding.
The milk cow of free-ranging experimental group 1, free-ranging experimental group 2 and free-ranging blank control group carries out free-ranging, without milk in free-ranging place
Other edible foods of ox；The milk cow of stable breeding experimental group 1, stable breeding experimental group 2 and stable breeding blank control group carries out stable breeding, without it in circle
His edible food.
Two, milk Data Detection
The acquisition of DHA milk sample: the milk samples of all feeding oxen three times, mixed sampling inspection are acquired in the morning, afternoon and evening；And according to national standard
Fatty acid determination in GB5413.27-2010 infant food and dairy products；Detect butterfat, the lactoprotein, DHA content in milk
Index.
As a result (table 3) is shown, either free-ranging or stable breeding, and the content of butterfat and lactoprotein in experimental group milk is same
Blank control group is without significant difference.And in feed add embodiment 1 split pot algae powder after, with the increase of feeding time, milk
In DHA content gradually increase, and DHA content is all remarkably higher than control group in each experimental group milk, in two control group milk
DHA content is without significant difference.
Table 3, milk DHA content testing result (mg/100g milk)
Note: pre-feeding period terminates to be pre-feeding period the 15th day, the 7th, 15,30,45,60 and 75 day formal phase be positive respectively formula nursing
The the 7th, 15,30,45,60 and 75 day of phase, refers to the 100th day after formal 75 days phases.
Embodiment 4, embodiment 2 the pot algae powder that splits the egg laying performance of egg quality and laying hen can be improved
The present embodiment splits pot algae powder by feed embodiment 2 to laying hen, detection split pot algae powder to Layer Production Performance and
The influence of egg quality.
1, experimental animal
Laying hen (sea orchid Cold boiled chicken) 360 in laying period of the healthy weight without significant difference is randomly selected, between each laying hen
Monthly age without significant difference, laying hen is randomly divided into 4 groups of (control group, 0.5% experimental group, 1.0% experimental group and 1.5% experiments
Group), every group 6 are parallel, and each parallel 15.
2, feeding management
Using 3 layers of cage, continuous light.Test daily ration is fed in the form of dry mash, and day feeds 3 times, is freely eaten and is drunk water,
Daily feed intake is recorded by cage.Experimental group laying hens essential ration is corn-soybean meal diet, and each experimental group feeding is added to implementation
The basal diet for splitting pot algae powder of example 2, the mass content that pot algae powder 1 is split in the food of 0.5% experimental group is 0.5%, and 1.0% is real
It tests that split the mass content of pot algae powder 1 in the food of group be 1.0%, the mass content of pot algae powder 1 is split in the food of 1.5% experimental group
It is 1.5%；Control group fed basal diet.Experimental group feeding is contained and is denoted as the 1st day on the day of splitting pot algae powder.Statistics produces daily
Egg rate respectively in the cholesterol level (the results are shown in Table 4 in the 25th day) of the 15th day and the 25th day detection egg, and calculates production
The variation of egg rate, egg cholesterol and DHA content, the results are shown in Table 5.
Table 4 splits pot algae powder 1 raising the 25th day laying rate, the measurement result of cholesterol and DHA content
Table 5, raising the 15th day and the 25th day laying rate and egg nutrient ingredient variation
Note: in table 5, " laying rate increase and decrease " refers to the variable quantity compared with the laying rate of control group same time laying hen, and " gallbladder is solid
Alcohol increase and decrease " refers to the variable quantity compared with the cholesterol level of control group same time egg, and "+" indicates to increase, and "-" expression subtracts
It is few.
The 25th day laying rate, egg size, feed intake, egg material ratio are counted, egg lipid content and cholesterol level are detected,
The results are shown in Table 6.
Same amount does not split the influence to performance in layers and lipid in pot algae powder the 25th day for table 6, raising
Significance analysis is carried out between projects each group (index), wherein the significance analysis of laying rate is as follows:
0.5% group of statistic
| Group | N | Mean value | Standard deviation | The standard deviation of mean value |
| Control group | 6 | 91.600 | 3.4774 | 1.4196 |
| 0.5% group | 6 | 87.617 | 4.4441 | 1.8143 |
0.5% group of independent sample is examined
1.0% group of statistic
| Group | N | Mean value | Standard deviation | The standard deviation of mean value |
| Control | 6 | 91.600 | 3.4774 | 1.4196 |
| 1.0% group | 6 | 95.600 | 1.8580 | .7585 |
1.0% group of independent sample is examined
1.5% group of statistic
| Group | N | Mean value | Standard deviation | The standard deviation of mean value |
| Control | 6 | 91.600 | 3.4774 | 1.4196 |
| 1.5% group | 6 | 93.500 | 2.1392 | .8733 |
1.5% group of independent sample is examined
From significance analysis as can be seen that for laying rate, the 25th day 0.5% group and 1.5% group of its laying rate relative to
Control group difference is not significant (P > 0.05), but 1.0% group of laying rate (P < 0.05) that can significantly improve laying hen, shows to raise
The laying rate of laying hen can be improved in the pot algae powder 1 that splits for feeding specific quantity.
Pot algae powder 1 is split in laying hen feeding, feeds the 15th day DHA content relative comparison group, 0.5% group can be improved
317.71%, reach 150mg/100g；1.0% group can be improved 579.48%, reach 244mg/100g；And 1.5% group can be with
885.79% is improved, 354mg/100g is reached.After feeding 25 days, 0.5% group and 1.0% group remains unchanged substantially, and 1.5% group
Have and slightly decline, but compared with the control group, still dramatically increases.Show that feeding splits pot algae powder 1 and DHA in laying hen egg can be improved
Content, and split that pot algae 1 additive amount of powder is more, and DHA content is higher in egg in food.
Pot algae powder 1 is split in laying hen feeding, and cholesterol content in eggs is decreased obviously；The slippage of the content of egg cholesterol
With the content of pot algae powder 1 is split in food without obvious relation, but be the increase of time with feeding, cholesterol content in eggs has into one
Walk downward trend.Show that feeding splits pot algae powder 1 and can reduce the content of cholesterol in laying hen egg.
According to the method described above, the pot algae powder 1 that splits in above-mentioned steps is replaced with and splits pot algae powder 2, other steps are constant, obtain
Arrived Similar trend as a result, showing that the pot algae powder 1 and 2 that splits of embodiment 1 all has identical function.
<110>Xiamen Huisheng Biological Co., Ltd.
<120>it splits pot algae and its preparation and is improving the application in laying rate of laying hen
<160> 2
<170> PatentIn version 3.5
<210> 1
<211> 207
<212> DNA
<213>artificial sequence
<220>
<223>
<400> 1
agccatgcat gtgtaagtat aagcgattgt actgtgagac tgcgaacggc tcattatatc 60
agtaataatt tcttcggtag tttcttttat atggatacct gcagtaattc tggaaataat 120
acatgctgta agagccctgt atggggctgc acttattaga ttgaagccga ttttattggt 180
gaatcatgat aattgagcag attgact 207
<210> 2
<211> 239
<212> DNA
<213>artificial sequence
<220>
<223>
<400> 2
gagttctgcc tctgtccaaa aattaatcca aacagaaaca tcccatggtt tcatcggacc 60
gttcaatcgg taggtgcgac gggcggtgtg tacaaagggc agggacgtat tcaatgcaag 120
ctgatgactt gcgtttacta ggaattcctc gttggagatt aataattgca aaaatctagc 180
cccagcacga tgagcgttcc aaggattagc caggccttcc gaccaagcac tcaattcca 239
Claims (10)
1. splitting pot algae (Schizochytrium limacinum) or following any applications of its preparation:
B1) application in animal product yield is being improved；
B2) the application in the substance that preparation improves animal product yield.
2. application according to claim 1, it is characterised in that: described to split pot algae (Schizochytrium limacinum)
It is described to split pot algae (Schizochytrium limacinum) to split pot algae (Schizochytrium limacinum) HS01
HS01 is CGMCC No.13746 in the deposit number of China Committee for Culture Collection of Microorganisms's common micro-organisms center；
And/or the animal is poultry.
3. application according to claim 2, it is characterised in that: the animal is chicken.
4. application according to claim 3, it is characterised in that: the chicken is Beijing Cold boiled chicken, the blue Cold boiled chicken in sea, the blue brown laying hen in sea
Or the blue powder shell chicken in sea.
5. according to the application any in claim 2-4, it is characterised in that: the animal product is what the animal produced
Egg.
6. any application in -5 according to claim 1, it is characterised in that: the preparation is to split pot algae powder.
7. any application in -6 according to claim 1, it is characterised in that: the preparation is according to the side included the following steps
Method preparation: pot algae (Schizochytrium limacinum) is split described in culture claim 1, obtains fermentation liquid；It utilizes
The preparation is prepared in the fermentation liquid.
8. application according to claim 7, it is characterised in that: split pot algae described in culture claim 1
(Schizochytrium limacinum) is carried out using fermentation medium, and the fermentation medium is made of solvent and solute,
The solvent is water, the solute and its concentration be respectively 60~150g/L of glucose, 8~25g/L of yeast extract, yeast powder 3~
8g/L、Na2SO45~20g/L, 0.5~1.5g/L of KCl, MgSO41.0~3.0g/L, K2SO40.5~2.5g/L, KH2PO4
1.0~2.0g/L, (NH4)2SO42.0~5.0g/L, CaCl20.5~2.5g/L, CuSO40.001~0.02g/L, ZnSO4
0.001~0.02g/L, 0.001~0.06g/L of biotin, 0.1~10g/L of starch and albumen powder 0~20g/L, pH is 4.5~
6.5。
9. application according to claim 7 or 8, it is characterised in that: described that the system is prepared using the fermentation liquid
Agent includes: the dry fermentation liquid, obtains the preparation.
10. improve animal product yield method, comprising: feeding animals split pot algae (Schizochytrium limacinum) or
Its preparation, which is realized, improves the animal product yield.
Priority Applications (7)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810300872.8A CN109077193B (en) | 2018-04-04 | 2018-04-04 | Application of schizochytrium limacinum and preparation thereof in improving laying rate of laying hens |
| PCT/CN2018/115327 WO2019192182A1 (en) | 2018-04-04 | 2018-11-14 | Application of schizochytrium limacinum and preparation thereof in improvement of quality and yield of animal product |
| JP2020529495A JP7191102B2 (en) | 2018-04-04 | 2018-11-14 | Use of Schizochytrium rimascinum and preparations thereof in improving the quality and yield of animal products |
| AU2018417303A AU2018417303A1 (en) | 2018-04-04 | 2018-11-14 | Application of schizochytrium limacinum and preparation thereof in improvement of quality and yield of animal product |
| EP18913727.6A EP3756472A4 (en) | 2018-04-04 | 2018-11-14 | Application of schizochytrium limacinum and preparation thereof in improvement of quality and yield of animal product |
| US17/043,468 US11583566B2 (en) | 2018-04-04 | 2018-11-14 | Use of Schizochytrium limacinum and its preparation in improving the quality and yield of animal product |
| AU2018102207A AU2018102207A4 (en) | 2018-04-04 | 2018-11-14 | Application of schizochytrium limacinum and preparation thereof in improvement of quality and yield of animal product |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810300872.8A CN109077193B (en) | 2018-04-04 | 2018-04-04 | Application of schizochytrium limacinum and preparation thereof in improving laying rate of laying hens |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN109077193A true CN109077193A (en) | 2018-12-25 |
| CN109077193B CN109077193B (en) | 2022-05-17 |
Family
ID=64839613
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810300872.8A Active CN109077193B (en) | 2018-04-04 | 2018-04-04 | Application of schizochytrium limacinum and preparation thereof in improving laying rate of laying hens |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109077193B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114134050A (en) * | 2021-12-01 | 2022-03-04 | 清远一生自然生物研究院有限公司 | Schizochytrium FR-908 for efficiently accumulating DHA and beta-carotene and preparation method and application thereof |
| CN115918802A (en) * | 2022-11-09 | 2023-04-07 | 重庆市三品功能食品研究院有限公司 | Chicken feed for producing DHA (docosahexaenoic acid) eggs suitable for pregnant infants and lactating mothers and application of chicken feed |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102160604A (en) * | 2011-03-01 | 2011-08-24 | 厦门汇盛生物有限公司 | Schizochytrium aggregatum powder additive and application thereof to seedling cultivation, aquiculture and culture of poultry and livestock |
| CN103719540A (en) * | 2013-12-26 | 2014-04-16 | 彭厚新 | Feed additive for increasing DHA (docosahexenoic acid) content of poultry eggs |
| CN106987528A (en) * | 2017-05-31 | 2017-07-28 | 厦门汇盛生物有限公司 | One plant production docosahexaenoic acid bacterium and its application |
-
2018
- 2018-04-04 CN CN201810300872.8A patent/CN109077193B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102160604A (en) * | 2011-03-01 | 2011-08-24 | 厦门汇盛生物有限公司 | Schizochytrium aggregatum powder additive and application thereof to seedling cultivation, aquiculture and culture of poultry and livestock |
| CN103719540A (en) * | 2013-12-26 | 2014-04-16 | 彭厚新 | Feed additive for increasing DHA (docosahexenoic acid) content of poultry eggs |
| CN106987528A (en) * | 2017-05-31 | 2017-07-28 | 厦门汇盛生物有限公司 | One plant production docosahexaenoic acid bacterium and its application |
Non-Patent Citations (1)
| Title |
|---|
| 陈秀丽，等: ""裂殖壶菌粉对蛋鸡生产性能、蛋品质、血清生化指标和蛋黄二十二碳六烯酸含量的影响"", 《动物营养学报》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114134050A (en) * | 2021-12-01 | 2022-03-04 | 清远一生自然生物研究院有限公司 | Schizochytrium FR-908 for efficiently accumulating DHA and beta-carotene and preparation method and application thereof |
| CN114134050B (en) * | 2021-12-01 | 2023-09-15 | 清远一生自然生物研究院有限公司 | Schizochytrium limacinum FR-908 capable of accumulating DHA and beta-carotene, and preparation method and application thereof |
| CN115918802A (en) * | 2022-11-09 | 2023-04-07 | 重庆市三品功能食品研究院有限公司 | Chicken feed for producing DHA (docosahexaenoic acid) eggs suitable for pregnant infants and lactating mothers and application of chicken feed |
Also Published As
| Publication number | Publication date |
|---|---|
| CN109077193B (en) | 2022-05-17 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Hossain et al. | Growth performance, meat yield, oxidative stability, and fatty acid composition of meat from broilers fed diets supplemented with a medicinal plant and probiotics | |
| Ibrahim et al. | Chemical composition, medicinal impacts and cultivation of camelina (Camelina sativa) | |
| KR101971984B1 (en) | Mixed probiotics using heat-treated whole fat soybean, method for preparing the same, and method for producing high quality Korean native steers meat by using the mixed probiotics | |
| CN109082381A (en) | Split the application of pot algae and its preparation in improving ruminant milk in DHA content | |
| KR101735759B1 (en) | Effect of fattening performance and meat quality for Hanwoo steers by dietary supplement of medical herb | |
| CN106879550A (en) | A kind of method for improving laying rate of laying hen | |
| EP3793373A1 (en) | Probiotic for poultry or cud-chewing animals | |
| CN109077193A (en) | It splits pot algae and its preparation and is improving the application in laying rate of laying hen | |
| CN109007295A (en) | A kind of Moringa feed addictive, preparation method and purposes | |
| CN104997120A (en) | Production technology of enteromorpha prolifra fermented beverage prepared by mixed bacteria asynchronous fermentation | |
| CN107094997A (en) | A kind of egg feedstuff | |
| KR101833043B1 (en) | Method for producing feed additive comprising fermented sea mustard and feed additive produced by the same method | |
| CN109077194A (en) | It splits pot algae and is improving the application in egg quality with pot algae preparation is split | |
| CN107259120A (en) | A kind of method that utilization linseed diet produces function mutton | |
| JP7191102B2 (en) | Use of Schizochytrium rimascinum and preparations thereof in improving the quality and yield of animal products | |
| CN105768107A (en) | Composition for improving memory and preparation method and application thereof | |
| CN109198253A (en) | For producing feed addictive and the application of high DHA content egg | |
| CN106173377B (en) | A kind of sweet potato residue fermented feed of ruminant and its application | |
| KR101341991B1 (en) | Korean cattle feed | |
| CN103211226B (en) | Punicic acid or the application of punicic acid compound in raising body in DHA content | |
| CN102742731A (en) | Biological feed additive capable of raising lean meat frequency of animals, its preparation method and application | |
| CN106754427A (en) | Thermophilic blue spore pore fungi new strains and its acclimation method | |
| Hasibuan et al. | The effect of herbal extracts and probiotic feeding on productivity and quality of broilers | |
| CN112825976A (en) | Method for improving egg quality of laying hens by using peony seed meal and application | |
| Moghadam et al. | Supplemental carbohydrase enzyme and methionine in broilers fed flaxseed: effects on growth performance, nutrient retention, muscle lipids, and jejunal morphology |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |