CN109053286A - A kind of preparation method of rice microorganism fertilizer - Google Patents
A kind of preparation method of rice microorganism fertilizer Download PDFInfo
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- CN109053286A CN109053286A CN201811046810.5A CN201811046810A CN109053286A CN 109053286 A CN109053286 A CN 109053286A CN 201811046810 A CN201811046810 A CN 201811046810A CN 109053286 A CN109053286 A CN 109053286A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
- C05C3/00—Fertilisers containing other salts of ammonia or ammonia itself, e.g. gas liquor
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Abstract
The invention belongs to biotechnology technical fields, and in particular to a kind of preparation method of rice microorganism fertilizer.The present invention is using coconut husk as support material, but coconut husk is fine and close, hole is undeveloped, therefore the present invention passes through salt acid soak, first is that improving the acidic-group inside coconut husk, second is that it is loose clean to its inside, utilize Steam explosion treatment, it can effectively improve gas porosity, and cellulose for keeping it internal etc. carries out decomposed, it is carbonized in advance again, then by be modified its surface using maleic anhydride, then it is combined with soybean protein, again under the catalytic action of catalyst, virtue drawing is carried out with albumen using mixed sugar to react, and nutritional ingredient is existed with chemical bond or the formation for forming compound, it not easily runs off, the iron ion that catalyst generates simultaneously can be used as microelement and be supplemented, can living environment effectively be provided for microorganism, improve microbe survival ability.
Description
Technical field
The invention belongs to biotechnology technical fields, and in particular to a kind of preparation method of rice microorganism fertilizer.
Background technique
Currently, the rice conventional fertilizer application application of nitrogenous fertilizer (especially lay particular stress on) mostly based on nitrogen, phosphorus, potash fertilizer, despise in, micro- fertilizer
Application, bring in agricultural production serious chemical fertilizer apply partially with the unbalanced problem of nutrition supply and demand, cannot sufficiently meet rice
Nutrition needed for growth.Fertilizer is the material base of increasing production of rice, applies fertilizer, especially Rational Application nitrogen, phosphorus, potassium, zinc, silicon
Fertilizer can increase substantially rice yield.By the rational application of fertilizer, on the one hand can coordinate demand of the paddy growth to nutrient with
The contradiction of fertilizer provision from soil, to achieve the goal of high yield and high quality；On the other hand less fertilizers input can be used, to obtain higher production
Amount and maximum economic benefit, realize the purpose of cost-saving synergistic.Fertilization of rice must be according to the growth and development characteristic of rice, fertilizer
Nutritional characteristic and regulation of fertilizer requirement are reasonably applied.
Rice uses microorganism fertilizer to cause crops to obtain spy with microbial life activity and its product as one kind in recent years
The living microorganisms product for determining fertilizer effect is widely noticed, it is in culture fertility, raising chemical fertilizer utilization ratio, inhibition crops counterweight
Absorption, purification and the rehabilitating soil of the harmful substances such as metal and pesticide, mention the decomposed utilization for promoting rice straw and municipal refuse
High rice quality has excellent effect.But microbial manure is living body fertilizer, its effect largely has mainly by what it contained
The vital movement of beneficial microorganism is completed.Only when these beneficial microbes are in the situation of vigorous breeding and metabolism
Under, substance conversion and beneficial metabolic product could be formed constantly.For many years, most processing methods of microbial manure only with
Finished product microbial inoculum is mixed with filler, but filler cannot provide good living environment to microorganism well, and microorganism is caused to lack
Few fermentating metabolism product, has seriously affected the fertilizer efficiency of microbial manure product, and microbial manure is poor to environmental suitability, uses
Microbial bacterial agent is mainly single microorganism fungus kind, it is difficult to restore Tiny ecosystem structure, and action time is short, be unable to satisfy rice
Grow the demand to fertilizer.
Summary of the invention
The technical problems to be solved by the invention: for microbial inoculum in current rice microorganism fertilizer and filler cannot well into
Row combines, and causes fertilizer efficiency to reduce, and the problem that action time is short, the present invention provides a kind of preparation sides of rice microorganism fertilizer
Method.
In order to solve the above technical problems, the present invention is using technical solution as described below:
A kind of preparation method of rice microorganism fertilizer, the preparation method include the following steps:
(1) take coconut husk to be crushed, be sieved, sieving particle is put into hydrochloric acid solution and is impregnated, is filtered, washing, by washings with
Additive 7~9:1 in mass ratio, which is put into ball mill, carries out ball milling, collects ball milling object；
(2) ball milling object is uniformly mixed with ethanol solution 4~6:1 in mass ratio, carries out Steam explosion treatment, collect steam blasting
Filter residue is collected in object, filtering；
(3) filter residue is put into retort and is carbonized in advance, collect pre- carbide, by pre- carbide, water 2~4:9 in mass ratio
It is put into reactor and mixes, modifying agent is added, additional amount is the 30~35% of pre- charing amount of substance, and stirring preheating adds pre-
5~7% ammonium persulfates of amount of substance are carbonized, heating is stirred to react；
(4) after the completion of reaction, it discharges, filtering collects filtrate, filtrate, water 2:7 in mass ratio are uniformly mixed, and adjusts
PH to 9.0~9.5 adds the soybean protein of filtrate quality 10~20%, stirs at 65~70 DEG C；
(5) after stirring, the mixed sugar of filtrate quality 9~13% is added and filtrate quality 0.6~1.2% is added
Catalyst, is warming up to 90~100 DEG C, stirs, cooling, spray drying, collects spray-dried product, then by spray-dried product with mix
Microorganism liquid 1:4~6 in mass ratio carry out mixing standing, freeze-drying, collect freeze-drying object to get rice microorganism fertilizer.
Additive is to mix by ammonium hydrogen carbonate, ammonium nitrate mass ratio 4~6:1~3 in the step (1).
The condition of steam blasting is 1.5~2.0MPa in the step (2), maintains pressure 70s.
The condition carbonized in advance in the step (3) is to be warming up to 120~130 DEG C using nitrogen protection with 10 DEG C/min, protected
70~90min of temperature, then 180~190 DEG C are warming up to, 1h is carbonized in advance.
Modifying agent is maleic anhydride in the step (3), butyl acrylate 7:1~2 in mass ratio mix.
Mixed sugar is sucrose in the step (5), glucose 2:1~3 in mass ratio mix.
Catalyst is ferric nitrate in the step (5).
The preparation of composite microbial thing liquid in the step (5) are as follows: according to parts by weight, take 180~220 parts of water, 40~43
Part yeast extract, 20~23 parts of peptones, 10~13 portions of sucrose, 6~9 parts of azotobacter chroococcums, 5~7 parts of fertile soil, stirring are mixed
It closes, stands, filtering collects filtrate, filtrate is mixed with polyglutamic acid 8~10:3 in mass ratio to get complex microorganism
Liquid.
The present invention is compared with other methods, and advantageous effects are:
(1) present invention is using coconut husk as support material, but coconut husk is fine and close, and hole is undeveloped, therefore the present invention passes through salt acid soak,
First is that the acidic-group inside coconut husk is improved, second is that it is loose clean to its inside, it, can be effective using Steam explosion treatment
Cellulose for improving gas porosity, and keeping it internal etc. carries out decomposed, increases active group, then carbonized in advance, improves
Then adsorptivity and activity itself utilize the horse of grafting then by be modified its surface using maleic anhydride
Carry out acid anhydrides to be combined with soybean protein, improve the upper nutritional ingredient of raw material, then under the catalytic action of catalyst, using mixed
It closes sugar to react with albumen progress virtue drawing, further increases the nutritional ingredient of raw material, and nutritional ingredient is with chemical bond or formation
The formation of compound exists, and not easily runs off, while the iron ion that generates of catalyst can be used as microelement and be supplemented, can be with
It is effective to provide living environment for microorganism, improve microbe survival ability；
(2) present invention is cultivated using microbial inoculum and the microorganism in fertile soil, first is that can increase microbial diversity, is improved
Fertilizer efficiency further increases fertilizer efficiency, is wrapped up finally by polyglutamic acid second is that obtaining abundant organic matter therein by culture
Processing, and polyglutamic acid can decompose generation nutriment, provide rice use, can also use, increase for microorganism
Action time.
Specific embodiment
Additive is ammonium hydrogen carbonate, ammonium nitrate mass ratio 4~6:1~3
Modifying agent is maleic anhydride, butyl acrylate 7:1~2 in mass ratio mix.
Mixed sugar is sucrose, glucose 2:1~3 in mass ratio mix.
Catalyst is ferric nitrate.
The preparation of composite microbial thing liquid are as follows: according to parts by weight, take 180~220 parts of water, 40~43 parts of yeast extracts, 20
~23 parts of peptones, 10~13 portions of sucrose, 6~9 parts of azotobacter chroococcums, 5~7 parts of fertile soil, are stirred, at 28~35 DEG C
20~26h is stood, filtering collects filtrate, filtrate is mixed with polyglutamic acid 8~10:3 in mass ratio to get compound micro-
Biological fluids.
A kind of preparation method of rice microorganism fertilizer, the preparation method include the following steps:
(1) it takes coconut husk to be crushed, crosses 200 meshes, sieving particle is put in 0.8mol/L hydrochloric acid solution and impregnates 5h, filters, washes
It washs to neutrality, washings and additive 7~9:1 in mass ratio is put into ball mill, ball milling 20min is carried out with 300r/min, receive
Collect ball milling object；
(2) ball milling object is uniformly mixed with ethanol solution 4~6:1 in mass ratio, carries out Steam explosion treatment, the item of steam blasting
Part is 1.5~2.0MPa, maintains pressure 70s, collects steam blasting object, and filter residue is collected in filtering；
(3) filter residue is put into retort and is carbonized in advance, the condition carbonized in advance is using nitrogen protection, with 10 DEG C/min heating
To 120~130 DEG C, 70~90min is kept the temperature, then be warming up to 180~190 DEG C, carbonize 1h in advance, collect pre- carbide, will carbonize in advance
Object, water 2~4:9 in mass ratio, which are put into reactor, mixes 10min, and modifying agent is added, and additional amount is the 30 of pre- charing amount of substance
~35%, 30min is preheated in 50 DEG C of stirrings, 5~7% ammonium persulfates of pre- charing amount of substance is added, is warming up to 70~80 DEG C,
It is stirred to react 4h；
(4) after the completion of reaction, it discharges, filtering collects filtrate, filtrate, water 2:7 in mass ratio are uniformly mixed, and adjusts
PH to 9.0~9.5 adds the soybean protein of filtrate quality 10~20%, in 65~70 DEG C of stirring 7h；
(5) after stirring, the mixed sugar of filtrate quality 9~13% is added and filtrate quality 0.6~1.2% is added
Catalyst is warming up to 90~100 DEG C, and stirring 3h, cooling, spray drying is collected spray-dried product, then by spray-dried product and mixed
It closes microorganism liquid 1:4~6 in mass ratio and carries out mixing standing 1h, freeze-drying collects freeze-drying object to get rice microorganism
Fertilizer.
Additive is ammonium hydrogen carbonate, ammonium nitrate 6:3 in mass ratio is mixed.
Modifying agent is maleic anhydride, butyl acrylate 7:2 in mass ratio is mixed.
Mixed sugar is sucrose, glucose 2:3 in mass ratio is mixed.
Catalyst is ferric nitrate.
The preparation of composite microbial thing liquid are as follows: according to parts by weight, take 220 parts of water, 43 parts of yeast extracts, 23 parts of peptones,
13 portions of sucrose, 9 parts of azotobacter chroococcums, 7 parts of fertile soil, are stirred, and 26h is stood at 35 DEG C, and filtering is collected filtrate, will be filtered
Liquid is mixed with polyglutamic acid 10:3 in mass ratio to get composite microbial thing liquid.
A kind of preparation method of rice microorganism fertilizer, the preparation method include the following steps:
(1) it takes coconut husk to be crushed, crosses 200 meshes, sieving particle is put in 0.8mol/L hydrochloric acid solution and impregnates 5h, filters, washes
It washs to neutrality, washings and additive 9:1 in mass ratio is put into ball mill, ball milling 20min is carried out with 300r/min, collect
Ball milling object；
(2) ball milling object is uniformly mixed with ethanol solution 6:1 in mass ratio, carries out Steam explosion treatment, the condition of steam blasting
For 2.0MPa, pressure 70s is maintained, collects steam blasting object, filter residue is collected in filtering；
(3) filter residue is put into retort and is carbonized in advance, the condition carbonized in advance is using nitrogen protection, with 10 DEG C/min heating
To 130 DEG C, 90min is kept the temperature, then is warming up to 190 DEG C, 1h is carbonized in advance, collects pre- carbide, by pre- carbide, water in mass ratio 4:
9 are put into reactor and mix 10min, modifying agent are added, additional amount is the 35% of pre- charing amount of substance, in 50 DEG C of stirrings preheatings
30min adds 7% ammonium persulfate of pre- charing amount of substance, is warming up to 80 DEG C, is stirred to react 4h；
(4) after the completion of reaction, it discharges, filtering collects filtrate, filtrate, water 2:7 in mass ratio are uniformly mixed, and adjusts
PH to 9.5 adds the soybean protein of filtrate quality 20%, in 70 DEG C of stirring 7h；
(5) after stirring, the mixed sugar of filtrate quality 13% is added and the catalyst of filtrate quality 1.2% is added,
It is warming up to 100 DEG C, stirs 3h, spray-dried product is collected in cooling, spray drying, then by spray-dried product and mixed microorganism liquid
1:6 in mass ratio carries out mixing and stands 1h, and freeze-drying collects freeze-drying object to get rice microorganism fertilizer.
Additive is ammonium hydrogen carbonate, ammonium nitrate mass ratio 5:2 is mixed.
Modifying agent is maleic anhydride, butyl acrylate 7:1 in mass ratio is mixed.
Mixed sugar is sucrose, glucose 2:2 in mass ratio is mixed.
Catalyst is ferric nitrate.
The preparation of composite microbial thing liquid are as follows: according to parts by weight, take 200 parts of water, 42 parts of yeast extracts, 22 parts of peptones,
12 portions of sucrose, 8 parts of azotobacter chroococcums, 6 parts of fertile soil, are stirred, and 23h is stood at 32 DEG C, and filtering is collected filtrate, will be filtered
Liquid is mixed with polyglutamic acid 9:3 in mass ratio to get composite microbial thing liquid.
A kind of preparation method of rice microorganism fertilizer, the preparation method include the following steps:
(1) it takes coconut husk to be crushed, crosses 200 meshes, sieving particle is put in 0.8mol/L hydrochloric acid solution and impregnates 5h, filters, washes
It washs to neutrality, washings and additive 8:1 in mass ratio is put into ball mill, ball milling 20min is carried out with 300r/min, collect
Ball milling object；
(2) ball milling object is uniformly mixed with ethanol solution 5:1 in mass ratio, carries out Steam explosion treatment, the condition of steam blasting
For 1.8MPa, pressure 70s is maintained, collects steam blasting object, filter residue is collected in filtering；
(3) filter residue is put into retort and is carbonized in advance, the condition carbonized in advance is using nitrogen protection, with 10 DEG C/min heating
To 125 DEG C, 73min is kept the temperature, then is warming up to 185 DEG C, 1h is carbonized in advance, collects pre- carbide, by pre- carbide, water in mass ratio 3:
9 are put into reactor and mix 10min, modifying agent are added, additional amount is the 33% of pre- charing amount of substance, in 50 DEG C of stirrings preheatings
30min adds 6% ammonium persulfate of pre- charing amount of substance, is warming up to 75 DEG C, is stirred to react 4h；
(4) after the completion of reaction, it discharges, filtering collects filtrate, filtrate, water 2:7 in mass ratio are uniformly mixed, and adjusts
PH to 9.5 adds the soybean protein of filtrate quality 15%, in 68 DEG C of stirring 7h；
(5) after stirring, the mixed sugar of filtrate quality 11% is added and the catalyst of filtrate quality 0.9% is added,
95 DEG C are warming up to, 3h is stirred, cooling, spray drying collects spray-dried product, then spray-dried product and mixed microorganism liquid are pressed
Mass ratio 1:5 carries out mixing and stands 1h, and freeze-drying collects freeze-drying object to get rice microorganism fertilizer.
Additive is ammonium hydrogen carbonate, ammonium nitrate 4:1 in mass ratio is mixed.
Modifying agent is maleic anhydride, butyl acrylate 7:1 in mass ratio is mixed.
Mixed sugar is sucrose, glucose 2:1 in mass ratio is mixed.
Catalyst is ferric nitrate.
The preparation of composite microbial thing liquid are as follows: according to parts by weight, take 180 parts of water, 40 parts of yeast extracts, 20 parts of peptones,
10 portions of sucrose, 6 parts of azotobacter chroococcums, 5 parts of fertile soil, are stirred, and 20h is stood at 28 DEG C, and filtering is collected filtrate, will be filtered
Liquid is mixed with polyglutamic acid 8:3 in mass ratio to get composite microbial thing liquid.
A kind of preparation method of rice microorganism fertilizer, the preparation method include the following steps:
(1) it takes coconut husk to be crushed, crosses 200 meshes, sieving particle is put in 0.8mol/L hydrochloric acid solution and impregnates 5h, filters, washes
It washs to neutrality, washings and additive 7:1 in mass ratio is put into ball mill, ball milling 20min is carried out with 300r/min, collect
Ball milling object；
(2) ball milling object is uniformly mixed with ethanol solution 4:1 in mass ratio, carries out Steam explosion treatment, the condition of steam blasting
For 1.5MPa, pressure 70s is maintained, collects steam blasting object, filter residue is collected in filtering；
(3) filter residue is put into retort and is carbonized in advance, the condition carbonized in advance is using nitrogen protection, with 10 DEG C/min heating
To 120 DEG C, 70min is kept the temperature, then is warming up to 180 DEG C, 1h is carbonized in advance, collects pre- carbide, by pre- carbide, water in mass ratio 2:
9 are put into reactor and mix 10min, modifying agent are added, additional amount is the 30% of pre- charing amount of substance, in 50 DEG C of stirrings preheatings
30min adds 5~7% ammonium persulfates of pre- charing amount of substance, is warming up to 70 DEG C, is stirred to react 4h；
(4) after the completion of reaction, it discharges, filtering collects filtrate, filtrate, water 2:7 in mass ratio are uniformly mixed, and adjusts
PH to 9.0 adds the soybean protein of filtrate quality 10%, in 65 DEG C of stirring 7h；
(5) after stirring, the mixed sugar of filtrate quality 9% is added and the catalyst of filtrate quality 0.6% is added, rises
Temperature stirs 3h to 90 DEG C, cooling, spray drying, collects spray-dried product, then spray-dried product and mixed microorganism liquid are pressed matter
Amount carries out mixing than 1:4 and stands 1h, and freeze-drying collects freeze-drying object to get rice microorganism fertilizer.
Comparative example 1 is roughly the same with embodiment 2, to mix biomass carbon and mixed microorganism liquid 1:5 in mass ratio
It closes and stands 1h, freeze-drying collects freeze-drying object to get rice microorganism fertilizer.
The commercially available rice microorganism fertilizer of comparative example 2.
The rice microorganism fertilizer of Examples 1 to 3 and comparative example 1~2 is subjected to experimental test, in rice transplanting period, according to every
20~25 kilograms of mu, is applied in paddy field, for rice in utilization, and according to rice different time, it can successively increase by 10~
15% amount, finally according to 147-88(meters of matter measuring methods of NY) measurement rice quality.
Test result such as table 1.
Table 1
It can to sum up obtain, rice microorganism fertilizer prepared by the present invention has good getting fat effect.
Claims (8)
1. a kind of preparation method of rice microorganism fertilizer, which is characterized in that the preparation method includes the following steps:
(1) take coconut husk to be crushed, be sieved, sieving particle is put into hydrochloric acid solution and is impregnated, is filtered, washing, by washings with
Additive 7~9:1 in mass ratio, which is put into ball mill, carries out ball milling, collects ball milling object；
(2) ball milling object is uniformly mixed with ethanol solution 4~6:1 in mass ratio, carries out Steam explosion treatment, collect steam blasting
Filter residue is collected in object, filtering；
(3) filter residue is put into retort and is carbonized in advance, collect pre- carbide, by pre- carbide, water 2~4:9 in mass ratio
It is put into reactor and mixes, modifying agent is added, additional amount is the 30~35% of pre- charing amount of substance, and stirring preheating adds pre-
5~7% ammonium persulfates of amount of substance are carbonized, heating is stirred to react；
(4) after the completion of reaction, it discharges, filtering collects filtrate, filtrate, water 2:7 in mass ratio are uniformly mixed, and adjusts
PH to 9.0~9.5 adds the soybean protein of filtrate quality 10~20%, stirs at 65~70 DEG C；
(5) after stirring, the mixed sugar of filtrate quality 9~13% is added and filtrate quality 0.6~1.2% is added
Catalyst, is warming up to 90~100 DEG C, stirs, cooling, spray drying, collects spray-dried product, then by spray-dried product with mix
Microorganism liquid 1:4~6 in mass ratio carry out mixing standing, freeze-drying, collect freeze-drying object to get rice microorganism fertilizer.
2. the preparation method of rice microorganism fertilizer according to claim 1, which is characterized in that additive in the step (1)
It is mixed for ammonium hydrogen carbonate, ammonium nitrate 4~6:1~3 in mass ratio.
3. the preparation method of rice microorganism fertilizer according to claim 1, which is characterized in that steam is quick-fried in the step (2)
Broken condition is 1.5~2.0MPa, maintains pressure 70s.
4. the preparation method of rice microorganism fertilizer according to claim 1, which is characterized in that carbonized in advance in the step (3)
Condition be to be warming up to 120~130 DEG C with 10 DEG C/min, keep the temperature 70~90min using nitrogen protection, then be warming up to 180~
190 DEG C, 1h is carbonized in advance.
5. the preparation method of rice microorganism fertilizer according to claim 1, which is characterized in that modifying agent in the step (3)
It is mixed for maleic anhydride, butyl acrylate 7:1~2 in mass ratio.
6. the preparation method of rice microorganism fertilizer according to claim 1, which is characterized in that mixed sugar in the step (5)
It is mixed for sucrose, glucose 2:1~3 in mass ratio.
7. the preparation method of rice microorganism fertilizer according to claim 1, which is characterized in that catalyst in the step (5)
For ferric nitrate.
8. the preparation method of rice microorganism fertilizer according to claim 1, which is characterized in that compound micro- in the step (5)
The preparation of biological fluids are as follows: according to parts by weight, take 180~220 parts of water, 40~43 parts of yeast extracts, 20~23 parts of peptones, 10
~13 portions of sucrose, 6~9 parts of azotobacter chroococcums, 5~7 parts of fertile soil, are stirred, stand, filtering, collect filtrate, by filtrate with
Polyglutamic acid 8~10:3 in mass ratio is mixed to get composite microbial thing liquid.
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| CN113912451A (en) * | 2021-11-19 | 2022-01-11 | 杨诗雨 | Organic fertilizer capable of improving soil and preparation method thereof |
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Application publication date: 20181221 |