CN109044244A - Microscope is peeped in one kind is matched with endoscope - Google Patents
Microscope is peeped in one kind is matched with endoscope Download PDFInfo
- Publication number
- CN109044244A CN109044244A CN201810702602.XA CN201810702602A CN109044244A CN 109044244 A CN109044244 A CN 109044244A CN 201810702602 A CN201810702602 A CN 201810702602A CN 109044244 A CN109044244 A CN 109044244A
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- microscope
- endoscope
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- peeping
- cell
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B1/00—Instruments for performing medical examinations of the interior of cavities or tubes of the body by visual or photographical inspection, e.g. endoscopes; Illuminating arrangements therefor
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B1/00—Instruments for performing medical examinations of the interior of cavities or tubes of the body by visual or photographical inspection, e.g. endoscopes; Illuminating arrangements therefor
- A61B1/06—Instruments for performing medical examinations of the interior of cavities or tubes of the body by visual or photographical inspection, e.g. endoscopes; Illuminating arrangements therefor with illuminating arrangements
- A61B1/0661—Endoscope light sources
- A61B1/0684—Endoscope light sources using light emitting diodes [LED]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B1/00—Instruments for performing medical examinations of the interior of cavities or tubes of the body by visual or photographical inspection, e.g. endoscopes; Illuminating arrangements therefor
- A61B1/06—Instruments for performing medical examinations of the interior of cavities or tubes of the body by visual or photographical inspection, e.g. endoscopes; Illuminating arrangements therefor with illuminating arrangements
- A61B1/07—Instruments for performing medical examinations of the interior of cavities or tubes of the body by visual or photographical inspection, e.g. endoscopes; Illuminating arrangements therefor with illuminating arrangements using light-conductive means, e.g. optical fibres
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Surgery (AREA)
- Physics & Mathematics (AREA)
- Engineering & Computer Science (AREA)
- Optics & Photonics (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Pathology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Biophysics (AREA)
- Heart & Thoracic Surgery (AREA)
- Medical Informatics (AREA)
- Radiology & Medical Imaging (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Microelectronics & Electronic Packaging (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
Matched with endoscope interior microscope is peeped the present invention relates to a kind of.More particularly to peeping microscope in a kind of light field fluorescence submicron order.It includes the components such as miniature object lens, optical fiber, fiber adapter, manual Z axis adjusting knob, fluorescent optical filter group, LED light source, video camera and adjustment structure, CMOS camera, PC control terminal and the control box of high-NA.Cell can be amplified 1000 times or more by the endoscope microlens of this submicron order.Submicron resolution, it can be seen that the metamorphosis of subcellular level, such as the direct variation of born of the same parents' device or karyomorphism can effectively observe the heteromorphism of tissue；Advantage is real time imagery, can directly differentiate position and the stage of carcinogenesis in body.Europe and domestic key lab observe for living body depth brain neurological motion, somatoscopy animal brain deep layer, the imaging of brain deep layer hippocampus neuron, and the functional area dendritic spines (less than 1 micron) of cell surface protrusion are high-visible.
Description
Technical field
Matched with endoscope interior microscope is peeped the present invention relates to a kind of.It is widely used in medicine and Neuscience is ground
Field is studied carefully, more particularly to peeping microscope in a kind of light field fluorescence submicron order.Microscope is peeped in this, can clearly be distinguished
The boundary of cancerous issue and normal tissue accomplishes precisely to perform the operation, and can with maximum magnitude retain the tissue of health, is conducive to postoperative extensive
It is multiple；It can effectively improve cancer diagnosis rate and cure rate, can be widely applied to the observation of living body depth brain neurological motion, push brain
Research.
Background technique
Endoscopy is the important diagnosis and treatment method of early detection cancer, but due to the limit of conventional endoscope imaging multiplying power
System will finally be made a definite diagnosis toward contact by living body puncture.Traditional pathology is long from biopsy to diagnosis out time, need through
Biopsy, freezing sample then slice are gone through, handles and be fixed to film-making on glass slide, HE contaminates piece, and pathologist sees piece in microscope
The process of diagnosis lasts long；And since pathologist work fatigue is possible to fail to pinpoint a disease in diagnosis during diagnosing, biopsy is sampled such as
Fruit cannot accurately get the tissue of lesions position, the limitation etc. of pathological section, and film-making and dye tablet quality etc. can all influence to diagnose
Conclusion.Present cancer is commonplace, and early discovery, early treatment major part cancer can cure.
Biopsy synchronous cannot be carried out with diagnosis and operation under traditional pathology mode, need to sample when biopsy, sample is sent
Inspection, pathological diagnosis, then perform the operation；Alimentary tumor patient needs to undergo an endoscopy and a surgical procedure, intermediate
Several days waiting time are needed, cannot be handled in time.
The present invention is that microscope is peeped in a submicron order, and accuracy of observation reaches 0.4 micron, does not need to puncture sampling, just
The living body that cancer cell may be implemented precisely detects.Conventional endoscope is broken through, needs not move through traditional pathology mode, when inspection can
To look into and diagnose in physical examination, while it being clearly apparent the tissue morphology of tumour, accurate judgement tumor boundaries, effectively auxiliary operative doctor
Precisely judgement and tumor resection tissue, avoid the multiple wound to patient.
Summary of the invention
As shown in Figure 1, peeping microscope in one kind is matched with endoscope.More particularly to a kind of light field fluorescence submicron order
Inside peep microscope.It includes object lens, optical fiber, fiber adapter, manual Z axis adjusting knob, fluorescent optical filter group, LED light source, takes the photograph
The components such as camera and adjustment structure, CMOS camera, PC control terminal and control box.
Diameter only has in 2.6 millimeters of endoscope lens, containing 7 groups of hand-made eyeglasses, passes through wireless connection, so that it may
To realize the accurate movement of camera lens in controlling terminal.In general, the enlargement ratio of conventional endoscope is about at 5 times or so,
And the endoscope microlens of this submicron order, then cell can be amplified 1000 times or more.Submicron resolution can be seen
To the variation of cell born of the same parents' device level, it is exactly the direct variation of subcellular form, can effectively observes the heteromorphism of tissue, advantage
It is real time imagery, can directly differentiates position and the stage of carcinogenesis in body.Europe and domestic key lab are for living
The observation of body depth brain neurological motion, somatoscopy animal brain cortex, the imaging of brain deep layer hippocampus, the function of cell surface protrusion
Energy region dendritic spines (less than 1 micron) are high-visible.
Detailed description of the invention
Attached drawing 1 is that the present invention constitutes component drawings；
Attached drawing 2 is the Cerebral Cortex and hippocampus nerve dendritic spines that the present invention observes；
Attached drawing 3 is the mouse brain depth cortical neuron and hippocampus neuron that the present invention observes
Attached drawing 4 is that (red is brain gliosis cytoma to the cancerous tissue form abnormity figure of the invention observed, green is that small colloid is thin
Born of the same parents)；
1. objective object lens；2. fiber adapter fiber adapter；3. fiber optical fiber；4. fiber adapter
Fiber adapter；5. fine Piezo Z axis piezoelectricity Z axis；6. manual drive corset Z axis focus
The manual Z axis adjusting knob of knob；7. fluore cube flourescent sheet group；8. epi illuminater fluorescence, which is fallen, penetrates illumination dress
It sets；9. LED light source LED light source；10. camera adapter camera adapter；11. CMOS camera
CMOS camera；12. Control Box controls box；13. Main unit master controller.
Specific embodiment
The dendritic spines (less than 1 micron) of Neuroscience Research experiment, the imaging of brain deep layer hippocampus, cell surface protrusion are clear
It is clear visible.The transmitting of dendritic spines responsible nerve signal is the region that the brain activities such as learning and memory emotion occur；Because in the present invention
It is high to peep the resolution of microscope, and can widely be made by Europe and domestic key lab's buying with real time imagery
With for the observation of living body depth brain neurological motion, somatoscopy animal brain deep layer.
The present invention can be very big for the promotion of neurology research, and huge promotion will be also generated for medical development and is made
With.This diameter only has in 2.6 millimeters of endoscope lens, containing 7 groups of hand-made eyeglasses, passes through wireless connection, so that it may
To realize the accurate movement of camera lens in controlling terminal.In general, the enlargement ratio of conventional endoscope is about at 5 times or so,
And the endoscope microlens of this submicron order, then cell can be amplified 1000 times or more.Submicron resolution can be seen
To the variation of cell level, it is exactly the direct variation of the form of cell, can effectively observes the heteromorphism of tissue, advantage is reality
When be imaged, can directly differentiate position and the stage of carcinogenesis in body.In addition to being used for cancer diagnosis, peeped in this submicron order
Mirror may also help in the accurate excision that doctor realizes cancer surgeries, and in cancer operation, doctor's most headache is exactly to determine
The boundary of cancer cell and normal cell, if cancerous issue excision is imperfect, remaining cancer cell also will continue to grow, and cut off
Excessively, then human body can be caused huge harm, is unfavorable for post-operative recovery.And it is this in peep microscope, can clearly distinguish cancer
The boundary for becoming tissue and normal tissue, accomplishes precisely to perform the operation.
Microscope shape very little is peeped in the present invention, precisely, doctor is when in use by the biopsy in enteron aisle mirror for optical fiber transmission
Channel does not need in addition to increase affiliated facility, will not damage to patient, can be widely applied to Minimally Invasive Surgery, carries out in body
Observation and operation imaging use.
Claims (6)
1. matched with endoscope interior microscope is peeped a kind of, it is characterised in that microscope is peeped in a kind of light field fluorescence submicron order,
Including including object lens, optical fiber, fiber adapter, manual Z axis adjusting knob, fluorescent optical filter group, LED light source, video camera and tune
The components such as whole structure, CMOS camera, PC control terminal and control box.
2. peeping microscope in one kind according to claim 1 is matched with endoscope, it is characterised in that diameter only has 2.6 millis
In the endoscope lens of rice, containing the hand-made eyeglass of multiple groups, cell can be amplified 1000 times or more.
3. peeping microscope in one kind according to claim 2 is matched with endoscope, it is characterised in that peeped in submicron order
Microscope, accuracy of observation reach 0.8 micron, do not need to puncture sampling, so that it may realize that the living body of cancer cell precisely detects.
4. peeping microscope in one kind according to claim 3 is matched with endoscope, it is characterised in that submicron resolution,
It can be seen that the variation of cell level, is exactly the direct variation of the form of cell, can effectively observe the heteromorphism of tissue.
5. peeping microscope in one kind according to claim 4 is matched with endoscope, it is characterised in that real time imagery, it can be with
Position and the stage of carcinogenesis are directly differentiated in body.
6. peeping microscope in one kind according to claim 4 is matched with endoscope, it is characterised in that be used for living body depth
Brain neurological motion observation, somatoscopy animal brain deep layer, the imaging of brain deep layer hippocampus, the dendritic spines of cell surface protrusion
(less than 1 micron) is high-visible.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810702602.XA CN109044244A (en) | 2018-07-02 | 2018-07-02 | Microscope is peeped in one kind is matched with endoscope |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810702602.XA CN109044244A (en) | 2018-07-02 | 2018-07-02 | Microscope is peeped in one kind is matched with endoscope |
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| Publication Number | Publication Date |
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| CN109044244A true CN109044244A (en) | 2018-12-21 |
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Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2507399Y (en) * | 2001-11-15 | 2002-08-28 | 杨志林 | Tumor recognition nerve endoscope |
| US20040210113A1 (en) * | 2003-03-31 | 2004-10-21 | Naoki Hasegawa | Magnifying image pickup unit for an endoscope, an endoscope for in vivo cellular observation that uses it, and endoscopic, in vivo cellular observation methods |
| CN101449963A (en) * | 2008-12-29 | 2009-06-10 | 浙江大学 | Laser co-focusing micro-endoscope |
| CN103134784A (en) * | 2013-02-05 | 2013-06-05 | 华中科技大学 | Optical fiber living body fluorescence excitation spectral imaging device |
| CN204120977U (en) * | 2014-07-21 | 2015-01-28 | 中国科学院遥感与数字地球研究所 | A kind of live body microscopy endoscopic spectrum imaging device |
| CN105167736A (en) * | 2015-08-13 | 2015-12-23 | 中国人民解放军第四军医大学 | Novel multi-modal gastroendoscope system |
| CN106859579A (en) * | 2017-01-26 | 2017-06-20 | 浙江大学 | A kind of fibre bundle confocal fluorescent endoscopic imaging method and device based on sub-pix |
-
2018
- 2018-07-02 CN CN201810702602.XA patent/CN109044244A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2507399Y (en) * | 2001-11-15 | 2002-08-28 | 杨志林 | Tumor recognition nerve endoscope |
| US20040210113A1 (en) * | 2003-03-31 | 2004-10-21 | Naoki Hasegawa | Magnifying image pickup unit for an endoscope, an endoscope for in vivo cellular observation that uses it, and endoscopic, in vivo cellular observation methods |
| CN101449963A (en) * | 2008-12-29 | 2009-06-10 | 浙江大学 | Laser co-focusing micro-endoscope |
| CN103134784A (en) * | 2013-02-05 | 2013-06-05 | 华中科技大学 | Optical fiber living body fluorescence excitation spectral imaging device |
| CN204120977U (en) * | 2014-07-21 | 2015-01-28 | 中国科学院遥感与数字地球研究所 | A kind of live body microscopy endoscopic spectrum imaging device |
| CN105167736A (en) * | 2015-08-13 | 2015-12-23 | 中国人民解放军第四军医大学 | Novel multi-modal gastroendoscope system |
| CN106859579A (en) * | 2017-01-26 | 2017-06-20 | 浙江大学 | A kind of fibre bundle confocal fluorescent endoscopic imaging method and device based on sub-pix |
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