CN109043125A - A kind of mushroom source dog grain phagostimulant and preparation method thereof - Google Patents
A kind of mushroom source dog grain phagostimulant and preparation method thereof Download PDFInfo
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Abstract
The present invention provides a kind of mushroom source dog grain phagostimulants, it is prepared by maillard reaction thing, maltodextrin, potassium sorbate, sodium pyrophosphate, phosphoric acid, the maillard reaction thing is made of mushroom enzymatic hydrolysis clear liquid, xylose, glycine, cysteine hydrochloride, glutamic acid, thiamine, yeast extract；The mushroom enzymatic hydrolysis clear liquid is made of new fresh mushroom, edible mushroom hydrolase and water.The present invention also provides the preparation methods of above-mentioned mushroom source dog grain phagostimulant.It can effectively improve the palatability of dog grain and the intestinal health of pet dog using phagostimulant of the invention.
Description
Technical field
The invention belongs to feed for pet fields, are related to a kind of dog grain phagostimulant, and specifically a kind of mushroom source dog grain lures
Eat agent and preparation method thereof.
Background technique
Phagostimulant is a kind of flavor additives useful for being usually used in animal feed, plays a part of to promote feed intake.With people couple
The concern of pet and love are higher and higher, and can pet eat healthy and the satisfied emphasis for having become dog owner's concern, this causes
Requirement of the pet food to flavor is especially prominent, therefore the warp for affecting a dog food of the quality high degree of dog grain phagostimulant
Ji benefit.
Dog grain phagostimulant is that flavor improving agent is also nutritional additive, and flavor substance is mainly by Maillard reaction shape
At portioned product can also enhance fragrance with flavor compound.And its nutritive value is mostly derived from the substrate and system of dog grain phagostimulant
The part nutritional additive added during standby.
Dog grain phagostimulant on the market mostly uses greatly animal protein to do raw material at present, mainly using the liver of all kinds of animals or
Different meats are cooked substrate and are reacted, and the smell of this kind of product is often difficult to be received by dog owner.Compared with animal protein, eat
The Resource Density of mycoprotein is high, and reproduction speed is fast, and easy making process, and sanitary condition is easier to be managed, and product fragrance is softer.And
Polysaccharide component in edible mushroom plays the role of adjusting enteric microorganism, to the healthy and beneficial of pet dog.
Summary of the invention
For above-mentioned technical problem in the prior art, the present invention provides a kind of mushroom source dog grain phagostimulant and its preparations
Method, described this mushroom source dog grain phagostimulant and preparation method thereof will solve what existing product smell was difficult to be received by people
Technical problem, the present invention can improve the intestinal health of pet dog, so that alleviating pet dog changes the enteron aisle generated when grain not
Adaptation.
The present invention provides a kind of mushroom source dog grain phagostimulants, are prepared by the raw material of following parts by weight:
75-85 parts of maillard reaction thing,
10-25 parts of maltodextrin,
0.2-0.6 parts of potassium sorbate,
0.1-0.2 parts of sodium pyrophosphate,
- 3 parts of phosphatase 11；
The maillard reaction thing is prepared by the raw material of following parts by weight:
Clear liquid 80-90 parts of mushroom enzymatic hydrolysis,
4-5 parts of xylose,
0.5-1.5 parts of glycine,
0.5-1.5 parts of cysteine hydrochloride,
0.5-1.5 parts of glutamic acid,
0.5-1.5 parts of thiamine,
1-3 parts of yeast extract；
The mushroom enzymatic hydrolysis clear liquid is prepared by the raw material of following parts by weight:
New 15-30 parts of fresh mushroom,
0.05-0.2 parts of edible mushroom hydrolase,
65-85 parts of water.
Further, mushroom enzymatic hydrolysis clear liquid is prepared by the raw material of following parts by weight: new 25 parts of fresh mushroom, food
With 0.15 part of bacterium hydrolase, 75 parts of water.
Further, the maillard reaction thing is prepared by the raw material of following parts by weight: mushroom digests clear liquid 90
Part, 4 parts of xylose, 1 part of glycine, 1 part of cysteine hydrochloride, 1 part of glutamic acid, 1 part of thiamine, 2 parts of yeast extract.
Further, the mushroom source dog grain phagostimulant is prepared by the raw material of following parts by weight: Maillard reaction
80 parts of object, 17 parts of maltodextrin, 0.5 part of potassium sorbate, 0.1 part of sodium pyrophosphate, 2.5 parts of phosphoric acid.
The present invention also provides a kind of preparation methods of above-mentioned mushroom source dog grain phagostimulant, include the following steps:
1) one prepare mushroom enzymatic hydrolysis clear liquid the step of, weigh according to parts by weight new fresh mushroom, edible mushroom hydrolase,
Water is ground into little particle after cleaning new fresh mushroom with clear water, water and smashed mushroom is sufficiently stirred, and heats and constant temperature exists
45-55 DEG C, and be that edible mushroom hydrolase is added, constant temperature enzyme digestion reaction 3-4 hours, reheats between 4-7 with edible acid for adjusting pH
To 85-95 DEG C of progress enzyme deactivation, constant temperature continues 10-20 minutes, is cooled to room temperature, with 7500-9000 revs/min of revolving speed high speed from
The heart 10-20 minutes, supernatant is taken to obtain the mushroom enzymatic hydrolysis clear liquid；
2) the step of preparation maillard reaction thing weighs mushroom enzymatic hydrolysis clear liquid, xylose, sweet ammonia according to parts by weight
Acid, cysteine hydrochloride, glutamic acid, thiamine, yeast extract, by the described mushroom enzymatic hydrolysis clear liquid, xylose, glycine,
Cysteine hydrochloride, glutamic acid, thiamine and yeast extract are sufficiently mixed in proportion, and constant temperature is anti-under the conditions of 80-105 DEG C
It answers 1-2 hours, the maillard reaction thing is obtained after cooling；
3) maillard reaction thing, maltodextrin, potassium sorbate, sodium pyrophosphate, phosphoric acid are weighed according to parts by weight, it will be described
Maillard reaction thing is added in a reaction vessel, and thermostatic control is added to Mei Lade between 50-80 DEG C, by maltodextrin
It in reactant, is added while stirring, carries out shearing 4-8 minutes with cutting head after completely dissolution, the sorb is added after defoaming
Sour potassium and sodium pyrophosphate simultaneously sufficiently dissolve, and are cooled to room temperature, and adjusting pH value with food grade phosphoric acid is between 2.5-3.5 to get perfume (or spice)
Mushroom source dog grain phagostimulant.
Further, the first time heating temperature is 50 DEG C, and described is heated to be 90 DEG C for the second time.
Further, pH value control when enzymatic hydrolysis is in 4-7, and preferable ph 5, centrifugal speed is 7500-9000 revs/min
Clock, preferably 8000 revs/min, centrifugation time are 10-20 minutes, preferably 15 minutes.
The raw material of mushroom source dog grain phagostimulant of the present invention is fresh mushroom, not dry product mushroom, and new fresh mushroom needs powder
It is broken into little particle or rotten shape reuses.It can ensure that reservation mushroom is peculiar while the protein ingredient in mushroom is fully utilized in this way
Flavor, enzymatic hydrolysis is carried out after furthermore crushing can more effectively extract peptide material therein, while the contact improved with water can fill
Divide the polysaccharide extracted in mushroom.
The present invention digests mushroom using specific edible mushroom hydrolase, different from traditional protease and cellulose
Enzyme is used in compounding, and edible mushroom hydrolase more rationally, can more effectively promote in water contact mushroom in the proportion of various enzymes
Polypeptide and amino acid.Furthermore it can effectively avoid mushroom enzymatic hydrolysate by being centrifugated this physical method extraction mushroom enzymatic hydrolysis clear liquid
The variation of middle amino acid sequence, and the suspended matter that can not digest of removal can make the state of final phagostimulant product more preferably, state is more
Stablize.
The present invention uses xylose to react as reduced sugar, and xylose is pentose, the reaction in Maillard reaction
Rate is higher than the hexose that glucose is representative, while effectively improving the efficiency of production preparation, is avoided that long-time high temperature
Reaction lead to part sulfur-bearing ingredient such as cysteine hydrochloride and thiamine overreaction in Maillard reaction, generate bad
Burning, improve aroma quality.Furthermore the present invention adopts yeast and extracts cream, and full lasting paste flavor and meat are fragrant and more sticky and equal
Even fluid state has fine improvement result to the fragrance of phagostimulant, stability and Product Status.
The present invention is sheared using maltodextrin and maillard reaction thing, can effectively promote mushroom source dog grain phagostimulant
Solid content promotes product consistency, improves Product Status, and furthermore maltodextrin is alternatively arranged as carrier, plays dilution, filling is made
With promotion product humidity resistance simultaneously increases fragrance stability.Sodium pyrophosphate has powerful attraction to cat and dog and appetite is promoted to make
With, a small amount of addition can be promoted phagostimulant palatability and resistance to mouth.Product pH value is controlled in acidity using phosphoric acid, on the one hand may be used
Meet dog for the preference of tart flavour mouthfeel, the generation of harmful bacteria on the other hand can be effectively suppressed.
The present invention is compared with existing technology, and technological progress is significant.It is ground behind this in the optimization of formula and product
The efficiency of hair preparation is significantly improved.Each reactant and reaction process parameter are carried out single factor test by preparation method of the invention
Analysis, designing screening by Plackett-Burman influences significant factor, designs response surface optimization by Box-Behnken
Each factor.The present invention examines the resistance to mouth and palatability of phagostimulant using single bowl of method and two bowls of methods in product efficacy evaluation and test
It tests.Simultaneously record the excrement situation of experimental dog and the intestinal health of experimental dog detected, especially by detection Bifidobacterium,
The change in count of lactobacillus, Escherichia coli and C.perfringens is realized.
Specific embodiment
Embodiment 1
In parts by weight, by 25 parts of new fresh mushrooms with clear water clean after be ground into little particle, by 70 parts of water with it is smashed
Mushroom is thoroughly mixed, and is heated to 50 DEG C, is 5.5 with edible acid for adjusting pH value, 0.1 part of edible mushroom hydrolase, constant temperature is added
Stirring 3 hours, is again heated to 95 DEG C of enzyme deactivations, continues 15 minutes, be cooled to room temperature, with 8500 revs/min of revolving speed high speed centrifugation
15 minutes, supernatant is taken to obtain the mushroom enzymatic hydrolysis clear liquid.
Mushroom described in taking 85 parts digests clear liquid, 4 parts of xyloses, 1 part of glycine, 1 part of cysteine hydrochloride, 1 part of paddy ammonia
Acid, 1 part of thiamine and 2 parts of yeast extracts are sufficiently mixed, the isothermal reaction 2 hours under the conditions of 95 DEG C, are obtained after cooling down slightly
The maillard reaction thing.
85 parts of maillard reaction things are taken, controls at 65 DEG C, 15 portions of maltodextrins is slowly added into Maillard reaction
It in object, is added while stirring, carries out high speed shear 5 minutes with cutting head after completely dissolution, should be noted the generation of control foam, disappear
0.3 part of potassium sorbate and 0.15 part of sodium pyrophosphate are added after bubble and sufficiently dissolves.It is cooled to room temperature, adjusts pH with food grade phosphoric acid
Value is 3.3.
Embodiment 2
In parts by weight, by 25 parts of new fresh mushrooms with clear water clean after be ground into little particle, by 60 parts of water with it is smashed
Mushroom is thoroughly mixed, and is heated to 55 DEG C, is 5.3 with edible acid for adjusting pH value, 0.15 part of edible mushroom hydrolase, constant temperature is added
Stirring 3.5 hours, is again heated to 90 DEG C of enzyme deactivations, continues 15 minutes, be cooled to room temperature, with 8000 revs/min of revolving speed high speed from
The heart 15 minutes, supernatant is taken to obtain the mushroom enzymatic hydrolysis clear liquid.
Mushroom described in taking 90 parts digests clear liquid, 4 parts of xyloses, 1 part of glycine, 1 part of cysteine hydrochloride, 0.5 portion of paddy
Propylhomoserin, 0.5 part of thiamine and 3 parts of yeast extracts are sufficiently mixed, the isothermal reaction 1 hour under the conditions of 100 DEG C, after cooling down slightly
Obtain the maillard reaction thing.
80 parts of maillard reaction things are taken, controls at 65 DEG C, 25 portions of maltodextrins is slowly added into Maillard reaction
It in object, is added while stirring, carries out high speed shear 5 minutes with cutting head after completely dissolution, should be noted the generation of control foam, disappear
0.2 part of potassium sorbate and 0.15 part of sodium pyrophosphate are added after bubble and sufficiently dissolves.It is cooled to room temperature, adjusts pH with food grade phosphoric acid
Value is 3.0.
Embodiment 3
In parts by weight, by 30 parts of new fresh mushrooms with clear water clean after be ground into little particle, by 65 parts of water with it is smashed
Mushroom is thoroughly mixed, and is heated to 53 DEG C, is 5 with edible acid for adjusting pH value, 0.2 part of edible mushroom hydrolase is added, constant temperature stirs
It mixes 4 hours, is again heated to 90 DEG C of enzyme deactivations, continue 15 minutes, be cooled to room temperature, with 9000 revs/min of revolving speed high speed centrifugation 10
Minute, take supernatant to obtain the mushroom enzymatic hydrolysis clear liquid.
Mushroom described in taking 90 parts digests clear liquid, 3 parts of xyloses, 1 part of glycine, 0.5 part of cysteine hydrochloride, 0.5 part
Glutamic acid, 1.5 parts of thiamines and 3 parts of yeast extracts are sufficiently mixed, the isothermal reaction 2 hours under the conditions of 90 DEG C, cooling slightly
The maillard reaction thing is obtained afterwards.
80 parts of maillard reaction things are taken, controls at 70 DEG C, 20 portions of maltodextrins is slowly added into Maillard reaction
It in object, is added while stirring, carries out high speed shear 5 minutes with cutting head after completely dissolution, should be noted the generation of control foam, disappear
0.2 part of potassium sorbate and 0.12 part of sodium pyrophosphate are added after bubble and sufficiently dissolves.It is cooled to room temperature, adjusts pH with food grade phosphoric acid
Value is 3.0.
Embodiment 4
In parts by weight, by 18 parts of new fresh mushrooms with clear water clean after be ground into little particle, by 82 parts of water with it is smashed
Mushroom is thoroughly mixed, and is heated to 55 DEG C, is 6 with edible acid for adjusting pH value, 0.1 part of edible mushroom hydrolase is added, constant temperature stirs
It mixes 4 hours, is again heated to 85 DEG C of enzyme deactivations, continue 20 minutes, be cooled to room temperature, with 7500 revs/min of revolving speed high speed centrifugation 20
Minute, take supernatant to obtain the mushroom enzymatic hydrolysis clear liquid.
Mushroom described in taking 80 parts digests clear liquid, 5 parts of xyloses, 1.5 parts of glycine, 1.5 parts of cysteine hydrochlorides, 1 part
Glutamic acid, 1.5 parts of thiamines and 3 parts of yeast extracts are sufficiently mixed, the isothermal reaction 2 hours under the conditions of 90 DEG C, cooling slightly
The maillard reaction thing is obtained afterwards.
85 parts of maillard reaction things are taken, controls at 70 DEG C, 15 portions of maltodextrins is slowly added into Maillard reaction
It in object, is added while stirring, carries out high speed shear 7 minutes with cutting head after completely dissolution, should be noted the generation of control foam, disappear
0.25 part of potassium sorbate and 0.12 part of sodium pyrophosphate are added after bubble and sufficiently dissolves.It is cooled to room temperature, is adjusted with food grade phosphoric acid
PH value is 2.9.
Embodiment 5
In parts by weight, by 25 parts of new fresh mushrooms with clear water clean after be ground into little particle, by 75 parts of water with it is smashed
Mushroom is thoroughly mixed, and is heated to 50 DEG C, is 5 with edible acid for adjusting pH value, 0.15 part of edible mushroom hydrolase is added, constant temperature stirs
It mixes 3.8 hours, is again heated to 90 DEG C of enzyme deactivations, continue 15 minutes, be cooled to room temperature, with 8000 revs/min of revolving speed high speed centrifugation
15 minutes, supernatant is taken to obtain the mushroom enzymatic hydrolysis clear liquid.
Mushroom described in taking 90 parts digests clear liquid, 4 parts of xyloses, 1 part of glycine, 1 part of cysteine hydrochloride, 1 part of paddy ammonia
Acid, 1 part of thiamine and 2 parts of yeast extracts are sufficiently mixed, the isothermal reaction 1.5 hours under the conditions of 95 DEG C, after cooling down slightly
To the maillard reaction thing.
80 parts of maillard reaction things are taken, controls at 65 DEG C, 17 portions of maltodextrins is slowly added into Maillard reaction
It in object, is added while stirring, carries out high speed shear 7 minutes with cutting head after completely dissolution, should be noted the generation of control foam, disappear
0.5 part of potassium sorbate and 0.11 part of sodium pyrophosphate are added after bubble and sufficiently dissolves.It is cooled to room temperature, adjusts pH with food grade phosphoric acid
Value is 3.0.
Comparative example 1
Comparative example 1 and embodiment 5 are the difference is that select two different protein sources as preparation dog grain phagostimulant
Substrate, comparative example select on the market the most common chicken gizzard prepared as substrate, embodiment 5 use perfume (or spice) of the present invention
Mushroom prepares dog grain phagostimulant as substrate.
In parts by weight, it is ground into gruel after 65 portions of fresh chicken gizzard being cleaned with clear water, by 35 parts of water and smashed chicken gizzard
It is thoroughly mixed, is heated to 55 DEG C, be 6.5 with edible acid for adjusting pH value, 0.55 part of compound protease, constant temperature stirring is added
2.7 hours, 90 DEG C of enzyme deactivations are again heated to, continues 15 minutes, is cooled to room temperature.
Chicken gizzard enzymolysis liquid described in taking 80 parts, 10 parts of water, 4 parts of xyloses, 1 part of glycine, 1 part of cysteine hydrochloride, 1 part
Glutamic acid, 1 part of thiamine and 2 parts of yeast extracts are sufficiently mixed, the isothermal reaction 1.5 hours under the conditions of 95 DEG C, cooling slightly
The maillard reaction thing of the chicken gizzard obtained afterwards.
The maillard reaction thing of 90 portions of chicken gizzard is taken, controls at 65 DEG C, it is anti-that 10 portions of maltodextrins is slowly added into Mei Lade
It answers in object, is added while stirring, carry out high speed shear 8 minutes with cutting head after completely dissolution, should be noted the generation of control foam,
0.5 part of potassium sorbate and 0.11 part of sodium pyrophosphate are added after defoaming and sufficiently dissolves.It is cooled to room temperature, is adjusted with food grade phosphoric acid
PH value is 3.0.
Palatability test
In the blank assay dog food for being not added with any phagostimulant, mushroom food calling prepared by 4% embodiment 5 is sprayed respectively
Chicken gizzard phagostimulant prepared by agent and comparative example 1, makes by dog grain Aa and dog grain Bb.Furthermore prepare blank assay dog food, be named as
Dog grain Cc, compares.
The small-sized dog of 40 different cultivars is chosen as experimental dog, age of experimental dog need to 1 one full year of life between 5 one full year of life and
Health continues 2 days without disease, every group of feeding test, the daily meal of feeding two (sooner or later every a meal, being spaced 8 hours).First group of experiment
In, experimental dog it is every meal simultaneously feed each 150 grams of dog grain Aa and dog grain Bb, every meal feeding time be 30 minutes, weigh after feeding
And the surplus of dog grain Aa and dog grain Bb are recorded, while observing and recording experimental dog to the first preference of dog grain.Second group real
It tests and palatability test is carried out to dog grain Bb and dog grain Cc using same procedure.
Grazing rate (%)=consumption dog index of flow amount/original dog index of flow amount × 100%
Tableware must be cleaned before each feeding, and prepares sufficient drinking water, fixed tableware to prevent overturning, hand over by when lower meal feeding
Tableware position is changed, the dog grain after every meal feeding must not be reused.
Mean value is carried out to test result and standard deviation calculates, and does one-way analysis of variance, first group and second group of experiment
As a result respectively as shown in table one, table two:
Table one:
Table two:
| Dog grain | Aa | Cc |
| Grazing rate | 68.265±5.551 | 19.283±4.796* |
| First preference number | 144 | 16* |
*: indicating that there were significant differences (p < 0.05, n=40)
As shown in Table 1, the dog grain for being added to mushroom source dog grain phagostimulant is all slightly lower on grazing rate and first preference number
In the dog grain for adding traditional chicken gizzard phagostimulant, but without significant difference.As shown in Table 2, it is added to mushroom source phagostimulant
Dog grain is all significantly higher than the dog grain for not adding phagostimulant on grazing rate and first preference number.By palatability test it is found that
Using mushroom source dog grain phagostimulant compared with traditional chicken gizzard dog grain phagostimulant, to the improvement aspect of dog grain palatability, although effect
It is slightly worse, but there is no marked difference.Furthermore it is compared with the dog food for being not added with phagostimulant it is found that the mushroom source dog grain lures
Food agent has significant attractant effect, can greatly promote the grazing rate of dog grain, improves first feeding preference quantity, dramatically increases
The palatability of dog grain.
Resistance to mouth test
In the blank assay dog food for being not added with any phagostimulant, the mushroom source for spraying 4% preparation of embodiment 5 respectively is lured
The chicken gizzard phagostimulant for eating agent and the preparation of comparative example 1, makes by dog grain Aa and dog grain Bb.Furthermore prepare blank assay dog food, name
For dog grain Cc, compare.
The small-sized dog of 24 same breeds is chosen as experimental dog, and is only divided into 3 groups with every group 8, the age of experimental dog needs
2 one full year of life between 3 one full year of life and health without disease.Feeding test continues 14 days, and the daily meal of feeding two is (sooner or later every a meal, interval 8
Hour).The every meal of first group of experimental dog feeds 200 grams of dog grain Aa, and every meal feeding time is 30 minutes, weighs and remembers after feeding
Record the surplus of dog grain Aa.Second group and the experiment of third group carry out resistance to mouth to dog grain Bb and dog grain Cc using same procedure and survey
Examination.
Grazing rate (%)=consumption dog index of flow amount/original dog index of flow amount × 100%
Tableware must be cleaned before each feeding, and prepares sufficient drinking water, fixed tableware to prevent overturning, hand over by when lower meal feeding
Tableware position is changed, the dog grain after every meal feeding must not be reused.
Mean value is carried out to test result and standard deviation calculates, and does one-way analysis of variance, three groups of group experimental result difference
It is as shown in Table 3:
Table three
Note: a, b, c expression longitudinal comparison are no, and there were significant differences (p < 0.05, n=8)；A, B, C indicate whether lateral comparison has
Significantly (p < 0.05, n=8).
As shown in Table 3, the dog grain Aa of the mushroom source dog grain phagostimulant is added in resistance to mouth test in 14 days,
It significantly decreases on grazing rate from the 3rd day, continue to the 12nd day and is once decreased obviously.In addition, with dog grain Bb and
Dog grain Cc is compared, and dog grain Aa is substantially less than dog grain Bb in the 1st, 2 day grazing rate, but is higher than dog grain Cc, but from the 3rd day to the
8 days, three kinds of dog grains did not had significant difference on grazing rate, and had used the grazing rate of the dog grain Aa of mushroom source phagostimulant slightly higher
In dog grain Bb and dog grain Cc.It seeing on the whole, dog grain Aa and dog grain Bb were just not significantly different on grazing rate from the 3rd day, and
The a little higher than dog grain Bb of the grazing rate of dog grain Aa, and from the 9th day, the grazing rate of dog grain Aa is significantly higher than dog grain Cc.In conclusion
The mushroom source dog grain phagostimulant does not have difference with traditional phagostimulant several, chicken gizzard dog grain on resistance to mouth, and for dog grain
The improvement of resistance to mouth is very significant.
Intestinal flora detection
While doing the test of resistance to mouth, the excrement progress enterobacteriaceae for collecting experimental dog for the 0th day, the 7th day and the 14th day is chosen
The test of group verifies influence of the dog grain phagostimulant to pet dog intestinal health, it is intended to when grain is changed in improvement not to pet dog enteron aisle
Adaptation.
The intestinal flora specifically includes two kinds of probiotics, respectively Bifidobacterium and lactobacillus and two kinds of nocuousness
Pathogenic bacteria, respectively Escherichia coli and C.perfringens.Detection method uses colony counting method, the wherein detection of Bifidobacterium
Method is carried out according to GB4789.34-2016, and the detection method of lactobacillus is carried out according to GB 4789.35-2016, Escherichia coli
Detection method is carried out according to GB 4789.3-2016, and the detection method of C.perfringens is carried out according to GB 4789.13-2012.
The clump count obtained to detection takes denary logarithm to calculate, and calculates mean value and standard deviation, carries out one-way analysis of variance,
As a result as shown in table four to table seven.
Table four:
Table five:
Table six:
Table seven:
Note: a, b, c expression longitudinal comparison are no, and there were significant differences (p < 0.05, n=8)；A, B, C indicate whether lateral comparison has
Significantly (p < 0.05, n=8).
By table four and table five it is found that the mushroom source dog grain phagostimulant has remarkable effect to the growth of lactobacillus, to double
Discrimination bacillus this there is no effect.In addition, traditional chicken gizzard dog grain phagostimulant has faint inhibiting effect to the growth of lactobacillus, the 14th
It plate count result is it is found that the lactobacillus quantity eaten in the experimental dog enteron aisle of the dog grain Bb of chicken gizzard phagostimulant is significantly low
In having eaten the experimental dog for being added to mushroom source phagostimulant dog grain Aa, or even also less than blank control group Cc.Lactobacillus is enteron aisle
Interior important probiotics has positive facilitation to intestinal health, can not only maintain intestinal microecology balance, promote nutrition
The absorption of substance, but also improve body's immunity, adjust blood lipid level and in terms of play important work
With.The growth of this lactobacillus and proliferation can be to the growths of the harmful intestinal tract bacterias such as Escherichia coli, clostridium and gram-negative anaerobic bacteria
It is inhibited.
By table six and table seven it is found that the mushroom source dog grain phagostimulant has persistently Escherichia coli and C.perfringens
Inhibiting effect, and after continuous feeding 14 days, the Escherichia coli in experimental dog enteron aisle and C.perfringens can be substantially reduced.So
And compared with control group Cc, the colicine eaten in the experimental dog enteron aisle of addition chicken gizzard phagostimulant dog grain Bb increases instead
Although not being obviously improved, there is certain negative influence to the intestinal health of pet dog.Enterobacteriaceae in enteron aisle
Bacterium mainly includes escherichia coli, enterobacter cloacae and Klebsiella Pneumoniae etc., this kind of bacterium in host immunity decline
It can lure the generation of host disease into.If position is bred other than enteron aisle, various infectious diseases such as respiratory tract, urine can be caused
Road, wound infection etc..Furthermore when C.perfringens is excessively bred in enteron aisle, the toxin for being metabolized generation be will cause in food
The diseases such as poison, enterotoxemia and necrotic enteritis.
In conclusion can get using mushroom source dog grain phagostimulant prepared by the present invention similar with traditional chicken gizzard phagostimulant
Palatability and resistance to mouth effect, but long-term consumption can improve the intestinal health of pet dog.
The above content is merely a preferred embodiment of the present invention, and is not used in the limitation present invention, can there is various modifications and variations.It is all
Any improvement, the replacement done within the scope of the spirit and principles in the present invention, are included within the scope of protection of the present invention.
Claims (7)
1. a kind of mushroom source dog grain phagostimulant, which is characterized in that be prepared by the raw material of following parts by weight:
75-85 parts of maillard reaction thing,
10-25 parts of maltodextrin,
0.2-0.6 parts of potassium sorbate,
0.1-0.2 parts of sodium pyrophosphate,
- 3 parts of phosphatase 11；
The maillard reaction thing is prepared by the raw material of following parts by weight:
Clear liquid 80-90 parts of mushroom enzymatic hydrolysis,
4-5 parts of xylose,
0.5-1.5 parts of glycine,
0.5-1.5 parts of cysteine hydrochloride,
0.5-1.5 parts of glutamic acid,
0.5-1.5 parts of thiamine,
1-3 parts of yeast extract；
The mushroom enzymatic hydrolysis clear liquid is prepared by the raw material of following parts by weight:
New 15-30 parts of fresh mushroom,
0.05-0.2 parts of edible mushroom hydrolase,
65-85 parts of water.
2. a kind of mushroom source dog grain phagostimulant according to claim 1, which is characterized in that the described mushroom enzymatic hydrolysis clear liquid by
The raw material of following parts by weight is prepared: new 25 parts of fresh mushroom, 0.15 part of edible mushroom hydrolase, 75 parts of water.
3. a kind of mushroom source dog grain phagostimulant according to claim 1, which is characterized in that the maillard reaction thing by
The raw material of following parts by weight is prepared: mushroom digests 90 parts of clear liquid, 4 parts of xylose, 1 part of glycine, cysteine hydrochloride 1
Part, 1 part of glutamic acid, 1 part of thiamine, 2 parts of yeast extract.
4. a kind of mushroom source dog grain phagostimulant according to claim 1, which is characterized in that the mushroom source dog grain food calling
Agent is prepared by the raw material of following parts by weight: 80 parts of maillard reaction thing, 17 parts of maltodextrin, 0.5 part of potassium sorbate, burnt phosphorus
0.1 part of sour sodium, 2.5 parts of phosphoric acid.
5. a kind of preparation method of mushroom source dog grain phagostimulant described in claim 1, which is characterized in that the present invention also provides
A kind of preparation method of above-mentioned mushroom source dog grain phagostimulant, includes the following steps:
1) one prepares the step of mushroom digests clear liquid, weighs new fresh mushroom, edible mushroom hydrolase, water according to parts by weight, will
New fresh mushroom is ground into little particle after being cleaned with clear water, and water and smashed mushroom are sufficiently stirred, and heats and constant temperature is in 45-55
DEG C, and be that edible mushroom hydrolase is added, constant temperature enzyme digestion reaction 3-4 hours, is again heated to 85- between 4-7 with edible acid for adjusting pH
95 DEG C of progress enzyme deactivations, constant temperature continue 10-20 minutes, are cooled to room temperature, with 7500-9000 revs/min of revolving speed high speed centrifugation 10-
20 minutes, supernatant is taken to obtain the mushroom enzymatic hydrolysis clear liquid；
2) the step of preparation maillard reaction thing weighs mushroom enzymatic hydrolysis clear liquid, xylose, glycine, half according to parts by weight
The mushroom is digested clear liquid, xylose, glycine, half Guang ammonia by cystine hydrochloride, glutamic acid, thiamine, yeast extract
Acid hydrochloride, glutamic acid, thiamine and yeast extract are sufficiently mixed in proportion, isothermal reaction 1-2 under the conditions of 80-105 DEG C
Hour, the maillard reaction thing is obtained after cooling；
3) maillard reaction thing, maltodextrin, potassium sorbate, sodium pyrophosphate, phosphoric acid are weighed according to parts by weight, and the beauty is drawn
Moral reactant is added in a reaction vessel, and thermostatic control is added to Maillard reaction between 50-80 DEG C, by maltodextrin
It in object, is added while stirring, carries out shearing 4-8 minutes with cutting head after completely dissolution, the potassium sorbate is added after defoaming
It with sodium pyrophosphate and sufficiently dissolves, is cooled to room temperature, adjusting pH value with food grade phosphoric acid is between 2.5-3.5 to get mushroom source
Dog grain phagostimulant.
6. a kind of preparation method of mushroom source dog grain phagostimulant according to claim 5, which is characterized in that described first
Secondary heating temperature is 50 DEG C, and described is heated to be 90 DEG C for the second time.
7. a kind of preparation method of mushroom source dog grain phagostimulant according to claim 5, which is characterized in that pH when enzymatic hydrolysis
Value is 5, and centrifugal speed is 8000 revs/min, and centrifugation time is 15 minutes.
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Application publication date: 20181221 |