CN109031641A - A kind of digitalized sliced sheet system and pathological section analysis method - Google Patents
A kind of digitalized sliced sheet system and pathological section analysis method Download PDFInfo
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- CN109031641A CN109031641A CN201810951299.7A CN201810951299A CN109031641A CN 109031641 A CN109031641 A CN 109031641A CN 201810951299 A CN201810951299 A CN 201810951299A CN 109031641 A CN109031641 A CN 109031641A
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
- G02B21/365—Control or image processing arrangements for digital or video microscopes
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/0004—Microscopes specially adapted for specific applications
- G02B21/0012—Surgical microscopes
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/0004—Microscopes specially adapted for specific applications
- G02B21/002—Scanning microscopes
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
- G02B21/361—Optical details, e.g. image relay to the camera or image sensor
Abstract
The embodiment of the present invention provides a kind of digitalized sliced sheet system and pathological section analysis method, the digitalized sliced sheet system includes microscope, displacement platform, driving device and data processing equipment, the microscope includes eyepiece and object lens, and the objective table for placing slice sample is provided on institute's translation stage；The driving device is separately connected with the data processing equipment and institute's translation stage, the objective table is located at the microscopical eyepiece, the driving device is used to drive the displacement of institute's translation stage so that the slice sample on objective table is moved to predeterminated position, to observe the slice sample by the eyepiece under the control of the data processing equipment.The present invention cooperates the accurately and fast scanning of mobile platform realization section sample by enabling the digitalized sliced sheet system to carry the microscope of a variety of different models to digitlization slice systems ingehious design.
Description
Technical field
The present invention relates to medical diagnosis technical fields, in particular to a kind of digitalized sliced sheet system and pathological section
Analysis method.
Background technique
Existing pathological section system mostly as shown in Figure 1, by microscope, camera, removable scanning platform, controller with
And the end PC slice scanning system composition, but microscope therein generally require it is micro- using three mesh bioelectronics of specific customization
Mirror, and be furnished with the object lens such as x10, x20, x40, x100, i.e., the microscope in existing pathological section system must be specific customization
, if user oneself has bought microscope and cannot use, so as to cause existing pathological section system availability
Difference, poor user experience.
Summary of the invention
In view of this, the present invention provides a kind of digitalized sliced sheet system and pathological section analysis method, can effectively solve the problem that
The above problem.
In order to achieve the above object, present pre-ferred embodiments provide a kind of digitalized sliced sheet system, and the digitlization is cut
Piece system includes microscope, displacement platform, driving device and data processing equipment, and the microscope includes eyepiece and object lens, institute
The objective table for placing slice sample is provided on translation stage；
The driving device is separately connected with the data processing equipment and institute's translation stage, in institute's translation stage
Objective table is located at the microscopical eyepiece, and the driving device under the control of the data processing equipment for driving institute
Translation stage displacement is so that the slice sample on objective table is moved to predeterminated position, to pass through the eyepiece to the slice
Sample is observed.
In the selection of present pre-ferred embodiments, institute's translation stage includes three-D displacement platform or two-dimension displacement platform
One of.
In the selection of present pre-ferred embodiments, institute's translation stage includes for realizing objective table in X-direction and Y
The X-axis and Y-axis of axis direction displacement, and for realizing the Z axis of focus adjustment, the X-axis and Y-axis respectively with the driving device
Connection.
In the selection of present pre-ferred embodiments, the driving device includes spindle motor.
In the selection of present pre-ferred embodiments, the digitalized sliced sheet system further includes photographic device, the camera shooting
Device is connect with the data processing equipment, and the photographic device is set at the microscopical eyepiece, the photographic device
For obtain the sample image of the section sample under the microscopical eyepiece visual field and be sent to the data processing equipment into
Row image procossing and analysis.
In the selection of present pre-ferred embodiments, the digitalized sliced sheet system further includes diagnosis and treatment terminal, the diagnosis and treatment
Terminal and the data processing equipment communicate to connect.
On the other hand, present pre-ferred embodiments also provide a kind of pathological section analysis method, applied to above-mentioned number
Change slice systems, the pathological section analysis method includes:
Based on the original state of institute's translation stage, the data processing equipment sends control according to default movement rule and refers to
It enables to the driving device；
The driving device operates according to the control instruction and institute's translation stage is driven to move so that positioned at described
Pathological section sample on the objective table of displacement platform is moved to predeterminated position according to the default movement rule；
The data processing equipment control photographic device acquires the pathological section under the microscopical eyepiece visual field
The sample image of the current location of sample is simultaneously sent to the data processing equipment and is analyzed.
In the selection of present pre-ferred embodiments, institute's translation stage includes three-D displacement platform or two-dimension displacement platform
One of.
In the selection of present pre-ferred embodiments, which comprises
When the photographic device completes the acquisition to the sample image of the different parts of the section sample and transmission, institute
It states data processing equipment and splicing is carried out to collected each sample image.
In the selection of present pre-ferred embodiments, the method also includes:
Section sample image after the completion of splicing is calculated and exported as the input of default Illnesses Diagnoses model is described
Medical features on section sample image, and illness present on the pathological section sample is analyzed according to the medical features.
Compared with prior art, the embodiment of the present invention provides a kind of digitalized sliced sheet system and pathological section analysis method,
Wherein, the digitalized sliced sheet system that the present invention provides can carry the microscope of a variety of different models, and then cooperate mobile platform
Realize the scanning to section sample.In addition, the present invention can be realized image scanning splicing and artificial intelligence auxiliary diagnosis, it can be significantly
Medical diagnosis efficiency is improved, saves the diagnosis time, it is ensured that diagnostic accuracy.
Detailed description of the invention
In order to illustrate the technical solution of the embodiments of the present invention more clearly, below will be to needed in the embodiment attached
Figure is briefly described, it should be understood that the following drawings illustrates only certain embodiments of the present invention, therefore is not construed as pair
The restriction of range for those of ordinary skill in the art without creative efforts, can also be according to this
A little attached drawings obtain other relevant attached drawings.
Fig. 1 is the structural schematic diagram of digitalized sliced sheet system in the prior art.
Fig. 2 is the structural schematic diagram of digitalized sliced sheet system provided in an embodiment of the present invention.
Fig. 3 is the frame structure schematic diagram of digitalized sliced sheet system provided in an embodiment of the present invention.
Fig. 4 is the flow diagram of pathological section analysis method provided in an embodiment of the present invention.
Fig. 5 is another flow diagram of pathological section analysis method provided in an embodiment of the present invention.
Icon: 10- digitalized sliced sheet system；11- microscope；12- displacement platform；13- driving device；14- data processing
Equipment；15- photographic device.
Specific embodiment
In order to make the object, technical scheme and advantages of the embodiment of the invention clearer, below in conjunction with the embodiment of the present invention
In attached drawing, technical scheme in the embodiment of the invention is clearly and completely described, it is clear that described embodiment is
A part of the embodiment of the present invention, instead of all the embodiments.The present invention being usually described and illustrated herein in the accompanying drawings is implemented
The component of example can be arranged and be designed with a variety of different configurations.
Therefore, the detailed description of the embodiment of the present invention provided in the accompanying drawings is not intended to limit below claimed
The scope of the present invention, but be merely representative of selected embodiment of the invention.Based on the embodiments of the present invention, this field is common
Technical staff's every other embodiment obtained without creative efforts belongs to the model that the present invention protects
It encloses.
It should also be noted that similar label and letter indicate similar terms in following attached drawing, therefore, once a certain Xiang Yi
It is defined in a attached drawing, does not then need that it is further defined and explained in subsequent attached drawing.
Fig. 2 and Fig. 3 are please referred to, is the structural representation for the digitalized sliced sheet system 10 that present pre-ferred embodiments provide
Figure, the digitalized sliced sheet system 10 include microscope 11, displacement platform 12, driving device 13 and data processing equipment 14, institute
Stating microscope 11 includes eyepiece and object lens, and the objective table for placing slice sample is provided on institute's translation stage 12.
The driving device 13 is separately connected with the data processing equipment 14 and institute's translation stage 12, and the displacement is flat
Objective table in platform 12 is located at the eyepiece of the microscope 11, and the driving device 13 is used in the data processing equipment 14
Control under driving institute's translation stage 12 be displaced so that the slice sample on objective table is moved to predeterminated position, to pass through
Eyepiece is stated to observe the slice sample.
Wherein, the present embodiment can be realized the digitlization by the aforementioned setting to above-mentioned digitalized sliced sheet system 10
Quick disassembly and installation in 10 use process of slice systems.Meanwhile the digitalized sliced sheet system 10 that the present invention provides can carry
Different types of microscope 11 carries out the scanning of section sample, greatly improves the practicability of digitalized sliced sheet system 10.
Further, since using microscope 11 and displacement platform 12 in the digitalized sliced sheet system 10 provided in the present embodiment
Detachable design, while can be achieved at the same time the scanning of multiple section samples, and can be realized scan slice with slice is loaded
It carries out.
In detail, difference according to actual needs, the microscope 11 can be selected but to be not limited to WMSC-1037 type micro-
Mirror, WMS-1087 research biomicroscope etc..In the present embodiment, the microscope 11 can directly carry institute's translation stage 12
Scanning, the observation to section sample can be realized.
Further, the driving device 13 is flat for driving the displacement under the control of the data processing equipment 14
Objective table in platform 12 is moved to predeterminated position, in order to be realized by the microscope 11 to the slice sample on the objective table
The observation of product.In the present embodiment, the actual type of the driving device 13 can according to the practical structures of institute's translation stage 12 into
Row is chosen.
For example, institute's translation stage 12 can be but not limited to three-D displacement platform or two-dimension displacement platform, when institute's rheme
When shifting platform 12 is three-D displacement platform, the three-D displacement platform may include for realizing objective table in X-direction and Y-axis side
Connect respectively with X-axis from the driving device 13 to displacement and Y-axis, and for realizing the Z axis of focus adjustment, the X-axis and Y-axis
It connects.
Here, the driving device 13 may include X-axis spindle motor, Y-axis spindle motor and Z axis spindle motor, the X
Axis spindle motor connect, the Y-axis lead screw electricity mobile in X-direction with driving objective table with the X-axis of the three-D displacement platform 12
Machine is connect with the Y-axis of the three-D displacement platform 12 and is moved in the Y direction with driving objective table, the Z axis spindle motor with it is described
The Z axis connection of three-D displacement platform 12 is mobile in Z-direction with driving objective table.When actual implementation, the objective table is in X-axis, Y
The movement of axis, Z-direction can also be realized by manually adjusting.It is understood that when the driving device 13 is by multiple motors
When composition, 14 equipment of data processing equipment can be realized the independent control to each motor.
In addition, as an implementation, institute's translation stage 12 can also be by track, the branch run along the track
Frame and the objective table being arranged on the bracket are constituted so that the bracket can under the driving of the driving device 13 band
It moves the objective table to move along the track, specifically, this example is herein with no restrictions.
Further, intelligent scanning, the image of section sample are adopted in order to be realized by the digitalized sliced sheet system 10
Collection and medical diagnosis on disease, in the present embodiment, the digitalized sliced sheet system 10 may also include photographic device 15, the photographic device
15 connect with the data processing equipment 14, and the photographic device 15 is set at the eyepiece of the microscope 11, the camera shooting
Device 15 is used to obtain the sample image of the section sample under the eyepiece visual field of the microscope 11 and is sent at the data
It manages equipment 14 and carries out image procossing and Illnesses Diagnoses.
Wherein, the sample image that the connection of the photographic device 15 and the data processing equipment 14 is used to scan is sent out
The data processing equipment 14 is given, what 14 one side of data processing equipment can be used for being acquired according to the photographic device 15
Sample image adjusts the moving direction of institute's translation stage 12, on the other hand can be by the sample that sends to the photographic device 15
Image is handled and analyzes illness present in section sample.In addition, the photographic device 15 is carrying out adopting for sample image
When collection, it can be and realized under the control of the data processing equipment 14, the present embodiment is not particularly limited herein.
Further, the data processing equipment 14 realizes control and data point to the driving device 13 as device
Analysis processing, when actual implementation, the data processing equipment 14 is that the electronics that there is computer etc. data processing and instruction to send is set
It is standby.Optionally, in the present embodiment, the data processing equipment 14 is also mountable has for carrying out section sample image analysis
Slice analysis system software etc., the present embodiment is herein with no restrictions.
Further, in the present embodiment, the digitalized sliced sheet system 10 may also include diagnosis and treatment terminal, and the diagnosis and treatment are whole
End is communicated to connect with the data processing equipment 14, and the diagnosis and treatment terminal can be Cloud Server, computer, mobile phone etc., for real
Now the data in the data processing equipment 14 are transmitted, or doctor convenient for distal end and expert pass through the diagnosis and treatment
Terminal carries out the illness consultation of doctors and discussion etc., so that the digitalized sliced sheet system 10 is not in practical applications by data processing equipment
The memory space limitation of 14 the machine or territory restriction etc..
Based on the design and description to above-mentioned digital slices system, the embodiment of the present invention also provides a kind of applied to the number
The pathological section analysis method of word slice systems 10, as shown in figure 4, the process for the pathological section analysis method is illustrated
Figure, is introduced below with reference to detailed process and step of the Fig. 4 to the pathological section analysis method.
Step 10, the original state based on institute's translation stage 12, the data processing equipment 14 is according to default mobile rule
Control instruction is then sent to the driving device 13；
Step 11, the driving device 13 operated according to the control instruction and drive institute's translation stage 12 mobile so that
The pathological section sample obtained on the objective table of institute's translation stage 12 is moved to default position according to the default movement rule
It sets.
Step 12, the data processing equipment 14 controls under the eyepiece visual field that photographic device 15 acquires the microscope 11
The sample image of the current location of the pathological section sample is simultaneously sent to the data processing equipment 14 and is analyzed.
The pathological section analysis method provided in above-mentioned steps S10- step S12 can be realized by data processing equipment 14
Intelligence, precision control to displacement platform 12, and then realized based on the collected sample image of photographic device 15 to pathology
The Accurate Analysis and diagnosis of section sample.It is understood that the movement of displacement platform 12 above-mentioned refers to institute's translation stage
The movement of objective table in 12, such as objective table are mobile in X-direction movement, Y-direction.
In detail, presetting movement rule described in step S10 and step S11 can be according to the actual type of the mobile platform
It is flexibly set, such as working as the mobile platform is two-dimensional movement platform, can first adjust objective table in X-axis according to predeterminated position
Direction is moved to preset, then adjusts objective table again in Y direction and is moved to preset, finally makes the slice on objective table
Sample displacement is to predeterminated position.
It is understood that the default movement rule can be the data processing equipment 14 according to institute's translation stage
12 current state automatically generates, and is also possible to artificially be manually entered, the present embodiment is limited herein.
By the cooperation of the eyepiece in photographic device 15 and the microscope 11 in step S12, to acquire pathological section sample
The sample image of product is simultaneously sent to the progress image procossing of data processing equipment 14, can be realized the precision point to section sample
Analysis.
Further, in actual implementation, in order to realize the intelligent analysis to the pathological section sample, in this implementation
In example, as shown in figure 5, the pathological section analysis method further includes step S13, it is specific as follows.
Step S13, the data processing equipment 14 judge whether to complete the sample of the different parts to the section sample
The acquisition of image carries out splicing to collected each sample image if all acquisitions.
Wherein, since under the amplification of the microscope 11, each collected sample image of the photographic device 15 is only
It is a part of the pathological section sample, therefore, in the present embodiment, in order to ensure the data processing equipment 14 is to described
The accuracy of pathological section sample analysis and comprehensive, the data processing equipment 14 are completed pair controlling the photographic device 15
After the Image Acquisition of the pathological section sample different parts, the sample image to different positions is needed to be spliced to obtain
Complete pathological section sample image.
Wherein, when splicing to each sample image, it can be the movement rule based on institute's translation stage 12
Image mosaic is realized with the acquisition order of sample image, is also possible to the actual sample based on pre-stored pathological section sample
Image realizes image mosaic etc., and the present embodiment is herein with no restrictions.
Further, it after completing sample image splicing in step s 13, can be carried out based on spliced section sample image
Illnesses Diagnoses specifically can refer to following step S14.
Step S14, using the section sample image after the completion of splicing as the input of default Illnesses Diagnoses model to calculate simultaneously
The medical features on the section sample image are exported, and is analyzed on the pathological section sample and is existed according to the medical features
Illness.
Image recognition can be carried out to the section sample image being spliced to form by image processing techniques in step S14,
And then based on illness present on pathological section sample described in the image characteristic analysis recognized.
When actual implementation, in step S14 when diagnosing to pathological image, diagnosis report can be generated according to diagnostic result
It accuses, and described diagnosis report etc. is uploaded to cloud platform or other medical systems, to breach the limitation of local storage space,
And the limitation of local level, expert's remote medical consultation with specialists can be carried out.
In conclusion the embodiment of the present invention provides a kind of digitalized sliced sheet system 10 and pathological section analysis method, wherein
The digitalized sliced sheet system 10 that the present invention provides can carry the microscope 11 of a variety of different models, and then cooperate mobile platform real
Now to the scanning of section sample.In addition, the present invention can be realized image scanning splicing and artificial intelligence auxiliary diagnosis, can mention significantly
High medical diagnosis efficiency saves the diagnosis time, it is ensured that diagnostic accuracy.
In several embodiments provided by the embodiment of the present invention, it should be understood that disclosed system and method, it can also
To realize by another way.System and method embodiment described above is only schematical, for example, in attached drawing
Flow chart and block diagram show that the systems of multiple embodiments according to the present invention, method and computer program product are able to achieve
Architecture, function and operation.In this regard, each box in flowchart or block diagram can represent module, a program
A part of section or code, a part of the module, section or code include that one or more is patrolled for realizing defined
Collect the executable instruction of function.It should also be noted that in some implementations as replacement, function marked in the box
It can occur in a different order than that indicated in the drawings.For example, two continuous boxes can actually be held substantially in parallel
Row, they can also be executed in the opposite order sometimes, and this depends on the function involved.It is also noted that block diagram and/or
The combination of each box in flow chart and the box in block diagram and or flow chart, can the function as defined in executing or dynamic
The dedicated hardware based system made is realized, or can be realized using a combination of dedicated hardware and computer instructions.
In addition, each functional module in each embodiment of the present invention can integrate one independent portion of formation together
Point, it is also possible to modules individualism, an independent part can also be integrated to form with two or more modules.
It, can be with if the function is realized and when sold or used as an independent product in the form of software function module
It is stored in a computer readable storage medium.Based on this understanding, technical solution of the present invention is substantially in other words
The part of the part that contributes to existing technology or the technical solution can be embodied in the form of software products, the meter
Calculation machine software product is stored in a storage medium, including some instructions are used so that a computer equipment (can be a
People's computer, electronic equipment or network equipment etc.) execute all or part of step of each embodiment the method for the present invention
Suddenly.And storage medium above-mentioned includes: USB flash disk, mobile hard disk, read-only memory (ROM, Read-Only Memory), deposits at random
The various media that can store program code such as access to memory (RAM, Random Access Memory), magnetic or disk.
It should be noted that, in this document, the terms "include", "comprise" or its any other variant are intended to the packet of nonexcludability
Contain, so that the process, method, article or equipment for including a series of elements not only includes those elements, but also including
Other elements that are not explicitly listed, or further include for elements inherent to such a process, method, article, or device.
In the absence of more restrictions, the element limited by sentence "including a ...", it is not excluded that including the element
Process, method, article or equipment in there is also other identical elements.
The foregoing is only a preferred embodiment of the present invention, is not intended to restrict the invention, for the skill of this field
For art personnel, the invention may be variously modified and varied.All within the spirits and principles of the present invention, made any to repair
Change, equivalent replacement, improvement etc., should all be included in the protection scope of the present invention.
Claims (10)
1. a kind of digitalized sliced sheet system, which is characterized in that the digitalized sliced sheet system includes microscope, displacement platform, drive
Dynamic device and data processing equipment, the microscope include eyepiece and object lens, are provided on institute's translation stage and cut for placing
The objective table of piece sample；
The driving device is separately connected with the data processing equipment and institute's translation stage, the loading in institute's translation stage
Platform is located at the microscopical eyepiece, and the driving device is used to drive institute's rheme under the control of the data processing equipment
Platform displacement is moved so that the slice sample on objective table is moved to predeterminated position, to pass through the eyepiece to the slice sample
It is observed.
2. digitalized sliced sheet system according to claim 1, which is characterized in that institute's translation stage includes that three-D displacement is flat
One of platform or two-dimension displacement platform.
3. digitalized sliced sheet system according to claim 1, which is characterized in that institute's translation stage includes for realizing load
The X-axis and Y-axis that object platform is displaced in x-axis direction and Y direction, and for realizing the Z axis of focus adjustment, the X-axis and Y-axis point
It is not connect with the driving device.
4. digitalized sliced sheet system according to claim 1, which is characterized in that the driving device includes spindle motor.
5. digitalized sliced sheet system according to claim 1, which is characterized in that the digitalized sliced sheet system further includes taking the photograph
As device, the photographic device is connect with the data processing equipment, and the photographic device is set to the microscopical eyepiece
Place, the photographic device are used to obtain the sample image of the section sample under the microscopical eyepiece visual field and are sent to described
Data processing equipment carries out image procossing and analysis.
6. digitalized sliced sheet system according to claim 1, which is characterized in that the digitalized sliced sheet system further includes examining
Terminal is treated, the diagnosis and treatment terminal and the data processing equipment communicate to connect.
7. a kind of pathological section analysis method, which is characterized in that applied to number described in any one of the claims 1-6
Change slice systems, the pathological section analysis method includes:
Based on the original state of institute's translation stage, the data processing equipment according to default movement rule send control instruction to
The driving device；
The driving device operates according to the control instruction and institute's translation stage is driven to move so that positioned at the displacement
Pathological section sample on the objective table of platform is moved to predeterminated position according to the default movement rule；
The data processing equipment control photographic device acquires the pathological section sample under the microscopical eyepiece visual field
Current location sample image and be sent to the data processing equipment and analyzed.
8. pathological section analysis method according to claim 7, which is characterized in that institute's translation stage includes three-D displacement
One of platform or two-dimension displacement platform.
9. pathological section analysis method according to claim 7, which is characterized in that the described method includes:
The multiple sample images for the pathological section sample different parts for acquiring and sending based on the photographic device, the number
Splicing is carried out to collected each sample image according to processing equipment.
10. pathological section analysis method according to claim 9, which is characterized in that the method also includes:
Using the section sample image after the completion of splicing as the input of default Illnesses Diagnoses model to calculate and export the slice
Medical features on sample image, and illness present on the pathological section sample is analyzed according to the medical features.
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| CN201810951299.7A CN109031641A (en) | 2018-08-21 | 2018-08-21 | A kind of digitalized sliced sheet system and pathological section analysis method |
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| CN201810951299.7A CN109031641A (en) | 2018-08-21 | 2018-08-21 | A kind of digitalized sliced sheet system and pathological section analysis method |
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| CN110648750A (en) * | 2019-09-04 | 2020-01-03 | 杭州憶盛医疗科技有限公司 | Digital section system and pathological section analysis method |
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Application publication date: 20181218 |