CN202141852U - Microscope device for full-view micro image shooting - Google Patents

Microscope device for full-view micro image shooting Download PDF

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Publication number
CN202141852U
CN202141852U CN201120255005U CN201120255005U CN202141852U CN 202141852 U CN202141852 U CN 202141852U CN 201120255005 U CN201120255005 U CN 201120255005U CN 201120255005 U CN201120255005 U CN 201120255005U CN 202141852 U CN202141852 U CN 202141852U
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China
Prior art keywords
microscope
image
micro
spindle motor
grating
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Expired - Lifetime
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CN201120255005U
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Chinese (zh)
Inventor
叶志前
章庆和
姜磊
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HANGZHOU ZHUOTENG INFORMATION TECHNOLOGY CO., LTD.
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ZHEJIANG GREEN LANDER INFORMATION TECHNOLOGY CO LTD
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Priority to CN201120255005U priority Critical patent/CN202141852U/en
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Abstract

A microscope device for full-view micro image shooting at least comprises a microscope, a computer and a rectification slide. An image shooting device is arranged on a trinocular connector of the microscope, an X-axis motor and a Y-axis motor are arranged on an object platform of the microscope, a grating scale in the X-axis direction and a grating scale in the Y-axis direction are arranged on the object platform of the microscope and respectively connected with a grating signal reading device, a Z-axis motor is arranged on a focusing knob of the microscope and connected with a focusing controller, and the image shooting device, the grating signal reading device and the focusing controller are respectively connected with the computer. On the premise that full-view micro image splicing accuracy is guaranteed, increased grating scale cost of the microscope device is much smaller than cost of a high-accuracy scanning platform and a high-accuracy location control system which are used before, so that cost of the whole microscope device is greatly lowered.

Description

The microscopie unit that a kind of panorama micro-image is taken
Technical field
The utility model relates to the microscopie unit that a kind of panorama micro-image is taken, and belongs to the microscopie unit technical field.
Background technology
At present external panorama micro-image is taken product comparative maturity, is to have adopted one of the method for lining by line scan (Progressive Scanning), linear array scanning method (Line scanning), piece transmission method (Area transference) and these four kinds of principles of optical matrix method (Optical matrix) to realize basically.But the price of these products is generally high, and making domesticly has the medium and low-end users of big demand to be difficult to accept to the micro-product of panorama.Owing to generally need use high precision scanning platform and positioning control system in the implementation structure of these products; And the cost of manufacture of precision sweep platform and control system is higher; Make that cost of goods manifactured is difficult to descend, thereby hindered the product promotion and application in market at home.
Summary of the invention
The purpose of the utility model is to overcome deficiency of the prior art, and a kind of microscopie unit of panorama micro-image shooting of low preparation cost is provided.
The technical scheme of the utility model is that the microscopie unit that a kind of panorama micro-image is taken which comprises at least a microscope and computing machine and proofreaied and correct slide; Said microscopical three order interfaces are provided with image capturing device; Said microscopical objective table is provided with X spindle motor and y-axis motor; Sample on the driving objective table moves at X axle and Y direction, and said microscopical objective table is provided with X-direction grating chi and Y direction grating chi; Measure the displacement of objective table in X axle and Y direction; Described X-direction grating chi is connected the grating signal reading device respectively with Y direction grating chi; Said microscopical focusing knob is provided with the Z spindle motor, the moving up and down of driving objective table, and described Z spindle motor connects focus controller; Focus controller drives the motion of Z spindle motor and to the focus control of objective table, described image capturing device, grating signal reading device and focus controller are connected computing machine respectively.
Described X spindle motor is connected X/Y spindle motor driving governor respectively with y-axis motor; X/Y spindle motor driving governor drives the motion of X spindle motor and y-axis motor; Described X/Y spindle motor driving governor connects computing machine.
Described correction slide adopts photoetching technique to make, and the pattern magnitude on the slide is corresponding with the object lens of different multiplying with the position.
The utility model can also provide the objective table of Z-direction accurately to locate up and down through increasing the grating chi of Z-direction.
The utility model is under the prerequisite that guarantees panorama micro-image splicing precision, and the cost that increases the grating chi is significantly less than the cost that uses high precision scanning platform and high-precision fixed level controlling system, thereby makes the cost of whole device descend greatly.
Description of drawings
Fig. 1 is the utility model structural representation.
Embodiment
Microscopie unit as shown in Figure 1, that a kind of panorama micro-image is taken which comprises at least a microscope 1 and computing machine 15 and correction slide 14; Three order interfaces 13 of said microscope 1 are provided with image capturing device 2; The objective table 11 of said microscope 1 is provided with X spindle motor 3 and y-axis motor 4, and the sample on the driving objective table 11 moves at X axle and Y direction; The objective table 11 of said microscope 1 is provided with X-direction grating chi 8 and Y direction grating chi 9, measures the displacement of objective table 11 in X axle and Y direction respectively; Described X-direction grating chi 8 is connected grating signal reading device 10 respectively with Y direction grating chi 9; The focusing knob 12 of said microscope 1 is provided with moving up and down of Z spindle motor 5 driving objective tables 11, and described Z spindle motor 5 connects focus controller 7; Focus controller 7 drives the motion of Z spindle motors and to the focus control of objective table; Described image capturing device 2, grating signal reading device 10 are connected computing machine 15 respectively with focus controller 7.
Described X spindle motor 3 is connected X/Y spindle motor driving governor 6 respectively with y-axis motor 4; X/Y spindle motor driving governor 6 drives the motion of X spindle motor 3 and y-axis motor 4; Described X/Y spindle motor driving governor 6 connects computing machine 15.
Described correction slide 14 adopts photoetching technique to make, and the pattern magnitude on the slide is corresponding with the object lens of different multiplying with the position.
The utility model also can improve the bearing accuracy that objective table moves up and down in Z-direction through increasing the grating chi 16 of Z-direction.Autofocus controller 7 reads the positional information of Z axle grating chi 16, and control objective table 11 moves up and down Z-direction, thereby can realize the bearing accuracy that objective table 11 moves on a large scale, the automation process of can realize placing section, changing section.
Before the use first of the utility model after installation, use special correcting circuit slide 14 to carry out learning correction,, set up the mapping relations of image pixel and space physics distance through the automatic identification of computer software.During actual photographed sample image; Computer software is according to the grating measuring data that read; According to the mapping relations that obtain in the learning correction process between image pixel and the space physics distance; Image to shooting obtains is cut out, the AP piece that the acquisition and the current shooting visual field are corresponding, and generation can be carried out the cell picture piece of panoramic picture splicing.
Be placed on sample on the objective table 11 with the moving of objective table X-Y direction, carry out the mobile control and the focus control up and down of X/Y direction, accomplish shooting whole sample zone through 15 pairs of objective tables of computing machine 11.The cell picture piece of the splicing that is useful on that computing machine 15 will obtain; The objective table physical location that records through X/Y direction grating chi during according to its image taking; Confirm the geometric space stitching position of cell picture piece, carry out the picture position splicing, thereby obtain the panorama micro-image.Under the measurement of high precision grating chi, guaranteed the accuracy of cutting out of photographic images, also guarantee to cut out the accuracy of the cell picture location of back cell picture piece in panorama micro-image splicing.
The learning correction process of the utility model device is a kind of computer picture recognition to the special pattern on the special correcting circuit slide 14.The image of the special correcting circuit slide 14 that diverse location is taken obtains the characteristic of diverse location through computer picture recognition.According to optical grating ruler measurement data, calculate mapping relations on the X axle, Y direction between image pixel and the space physics distance, and remembered by this device at diverse location.To the object lens of different multiplying, all to carry out learning correction in the same way.This device feature of replacing is not taking place or reinstalling under the situation of this device, the utility model device needn't carry out learning correction once more.
Sample is focused into sharply defined image under the control of focus controller after correctly being placed into objective table.Take image in this visual field, and read optical grating ruler measurement value that should position, the visual field.The image that shooting obtains is transferred to computing machine and is preserved.At X-direction moving stage 11, keep the Y direction invariant position, displacement is less than the X-direction size of taking field range.The optical grating ruler measurement value of this position is read in automatic focus and take this images of positions once more.Utilize this image taking position X-direction grating chi 8 measured values and last time the image taking position X-direction grating chi 8 measured values; According to the mapping relations on X-direction between image pixel that obtains in the learning correction process and the space physics distance; Image to current shooting carries out cutting out of directions X; Generate corresponding this and take the sliceable cell picture piece of physical location, and preserved separately.The shooting process of repetition X-direction and processing procedure are until the least significant end of X axle coverage.After having taken the last piece image of X-direction, keep the X axle constant, at Y direction moving stage 11, displacement is less than the Y direction size of taking field range.After automatic focus, take this location drawing picture, and read the optical grating ruler measurement value of this position.According to the mapping relations on Y direction between image pixel that obtains in the learning correction process and the space physics distance; According to these Y direction of principal axis grating chi 9 measured values with image taking position last time; And be combined on the Y direction and the adjacent existing image information of this photographic images; The image that this shooting is obtained carries out cutting out of Y direction, generates corresponding this and takes the sliceable cell picture piece of physical location, and preserved separately.Keep the Y direction invariant position once more, along X axle opposite direction moving stage, same displacement is less than the X-direction size of taking field range.Automatic focus and photographic images read the optical grating ruler measurement value of this camera site once more.According to the mapping relations on X-direction between the image pixel that obtains in the learning correction process and the space physics distance,, the image of current shooting is carried out cutting out of directions X according to these X-direction grating chi 8 measured values with image taking position last time.While is according to Y direction grating chi 9 measured values of this camera site; Be combined in Y direction and the adjacent image information of this photographic images; According to the mapping relations on Y direction between image pixel and the space physics distance, this photographic images is carried out cutting out of Y direction.Final this photographic images is created on all cell picture pieces through cutting out of X axle, Y direction, and is preserved separately.Keep the Y direction invariant position, repeat shooting process in the other direction and image cutting-out is crossed the least significant end of title until X axle coverage along the X axle.X axle shooting least significant end is the same with arriving last time, keeps the X-direction invariant position once more, along the Y direction moving stage.So repeat, form the shooting process of the similar scanning that moves around line by line, all sample zones have been taken the most at last, form the image block of corresponding each camera site.
When generating panoramic picture; The camera site corresponding according to every width of cloth cell picture piece; Image is carried out preliminary geometric position splicing; And adopt location matches algorithm and fusion treatment that the edge of adjacent two width of cloth image blocks is carried out fine adjustment, and eliminate the measuring error that factors such as machinery because of objective table, grating chi, electronics cause, splice micro-image thereby formed a complete accurate panorama.Since the position of every width of cloth cell picture piece with cut out size by the strict control of the high precision measuring data of grating chi; Guaranteed the accuracy of panorama micro-image splicing; Significantly reduce the fine adjustment computing time at image mosaic edge, satisfying the high-quality panorama micro-image of acquisition under the panorama requirement of splicing time.

Claims (5)

1. the microscopie unit that the panorama micro-image is taken which comprises at least a microscope (1), computing machine (15) and has proofreaied and correct slide (14); The three order interfaces (13) that it is characterized in that said microscope (1) are provided with image capturing device (2); The objective table (11) of said microscope (1) is provided with X spindle motor (3) and y-axis motor (4); The objective table (11) of said microscope (1) is provided with X-direction grating chi (8) and Y direction grating chi (9); Described X-direction grating chi (8) is connected grating signal reading device (10) respectively with Y direction grating chi (9); The focusing knob (12) of said microscope (1) is provided with Z spindle motor (5); Described Z spindle motor (5) connects focus controller (7); Described image capturing device (2), described grating signal reading device (10) and focus controller (7) are connected computing machine (15) respectively.
2. the microscopie unit that panorama micro-image according to claim 1 is taken is characterized in that described X spindle motor (3) is connected X/Y spindle motor driving governor (6) respectively with y-axis motor (4); Described X/Y spindle motor driving governor (6) connects computing machine (15).
3. the microscopie unit that panorama micro-image according to claim 1 is taken is characterized in that described correction slide (14) adopts photoetching technique to make, and the pattern magnitude of proofreading and correct on the slide (14) is corresponding with the object lens of different multiplying with the position.
4. the microscopie unit that panorama micro-image according to claim 1 is taken is characterized in that described image capturing device (2) is CCD or cmos digital camera.
5. the microscopie unit that panorama micro-image according to claim 1 is taken, its characteristic can also increase the grating chi (16) of Z-direction, and said Z axle grating chi (16) is connected with focus controller (7).
CN201120255005U 2011-07-19 2011-07-19 Microscope device for full-view micro image shooting Expired - Lifetime CN202141852U (en)

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Application Number Priority Date Filing Date Title
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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105181538A (en) * 2015-10-20 2015-12-23 丹东百特仪器有限公司 Granularity and particle form analyzer with scanning and splicing functions for dynamic particle image and method
CN108827146A (en) * 2018-06-14 2018-11-16 苏州富莱智能科技有限公司 TWAIN standard micrometering system and measurement method
CN108918519A (en) * 2018-07-05 2018-11-30 深圳辉煌耀强科技有限公司 A kind of cell smear image obtains and analysis system
CN109031641A (en) * 2018-08-21 2018-12-18 北京青燕祥云科技有限公司 A kind of digitalized sliced sheet system and pathological section analysis method

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105181538A (en) * 2015-10-20 2015-12-23 丹东百特仪器有限公司 Granularity and particle form analyzer with scanning and splicing functions for dynamic particle image and method
CN105181538B (en) * 2015-10-20 2018-06-26 丹东百特仪器有限公司 With scanning, the dynamic particle image granularity particle shape analyzer of splicing and method
CN108827146A (en) * 2018-06-14 2018-11-16 苏州富莱智能科技有限公司 TWAIN standard micrometering system and measurement method
CN108918519A (en) * 2018-07-05 2018-11-30 深圳辉煌耀强科技有限公司 A kind of cell smear image obtains and analysis system
CN109031641A (en) * 2018-08-21 2018-12-18 北京青燕祥云科技有限公司 A kind of digitalized sliced sheet system and pathological section analysis method

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Effective date of registration: 20121213

Address after: 310003 No. 566, No. 402, Xiacheng District, Hangzhou, Zhejiang, Zhongshan North Road

Patentee after: Ye Zhiqian

Address before: 310012, room two, building five, building 203, building 408, Wensanlu Road, Xihu District, Hangzhou, Zhejiang

Patentee before: Zhejiang Green Lander Information Technology Co.,Ltd.

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