CN109030519A - A kind of electron cryo-microscopy can operational observations recording device - Google Patents
A kind of electron cryo-microscopy can operational observations recording device Download PDFInfo
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- CN109030519A CN109030519A CN201810622825.5A CN201810622825A CN109030519A CN 109030519 A CN109030519 A CN 109030519A CN 201810622825 A CN201810622825 A CN 201810622825A CN 109030519 A CN109030519 A CN 109030519A
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- liquid nitrogen
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N23/00—Investigating or analysing materials by the use of wave or particle radiation, e.g. X-rays or neutrons, not covered by groups G01N3/00 – G01N17/00, G01N21/00 or G01N22/00
Abstract
The present invention relates to biological electron microscope fields, in particular be a kind of electron cryo-microscopy can operational observations recording device, liquid nitrogen feedway, vacuum plant, experimental vehicle, manual operation device and low temperature shoot recording equipment, the liquid nitrogen feedway is fixedly connected on the front end of experimental vehicle, vacuum plant is fixedly connected on the rear end of experimental vehicle, liquid nitrogen feedway is connected to experimental vehicle with vacuum plant, manual operation device is fixedly connected on the left end of experimental vehicle, low temperature shoots recording equipment clearance fit in the upper end of experimental vehicle, manual operation device and low temperature shooting recording equipment are arranged in experimental vehicle；Beneficial effects of the present invention provide the environment of a relative vacuum for freezing biological sample, guarantee freezing environment, pass through vacuum insulating simultaneously, guarantee experiment safety, simultaneously can in all directions multi-angle adjustment sample shooting angle, guarantee shooting quality, ensures the comprehensive of observation, while reducing liquid nitrogen in vaporescence to the influence of shooting.
Description
Technical field
The present invention relates to biological electron microscope fields, be in particular a kind of electron cryo-microscopy can operational observations record dress
It sets.
Background technique
The use of the Electronic Speculum open visual field of bioengineering well, makes very big contribution for bioengineering.Patent No.
CN201610617959.9's discloses a kind of scanning/transmission electron microscope association analysis vacuum moving device, including with
The vacuum cavity that scanning electron microscope is connected, internal two-dimension translational platform and transmission electron microscope sample bar eccentric sleeve；Vacuum chamber
Body front end and scanning electron microscope cavity flanged joint；Vacuum cavity is provided with the external knob of two-dimension translational platform；Inside cavity is equipped with
Transmission electron microscope sample bar eccentric sleeve for realizing the two-dimension translational platform that SEM electron beam imaging region accurately selects, and thereon,
Realize the Z axis fine tuning of imaging region.The high-resolution imaging and regional area high-precision ingredient of present invention combination TEM obtains and SEM
The advantages of pattern and ingredient of a wide range of sample can be obtained, is realized and is accurately positioned sample survey region, microcosmic, nanoscale phase
In conjunction with the microstructure of study sample, ingredient, object equivalent information；Simultaneously can be used for transmission electron microscope heat, be powered, liquid and
The preliminary test of its integrated specimen holder, to ensure the safety of transmission electron microscope.But the operation freedom degree of this device is not high, nothing
Method ensures the comprehensive of shooting angle, while liquefied nitrogen also will affect shooting visual angle.
Summary of the invention
The object of the present invention is to provide a kind of electron cryo-microscopy can operational observations recording device, its advantages be freezing
Biological sample provides the environment of a relative vacuum, guarantees freezing environment, while by vacuum insulating, guaranteeing experiment safety, together
When can in all directions multi-angle adjustment sample shooting angle, guarantee shooting quality, ensure the comprehensive of observation, while reducing liquid nitrogen
To the influence of shooting in vaporescence.
The purpose of the present invention is achieved through the following technical solutions:
The object of the present invention is to provide a kind of electron cryo-microscopy can operational observations recording device, including liquid nitrogen supply dress
It sets, vacuum plant, experimental vehicle, manual operation device and low temperature shooting recording equipment, the liquid nitrogen feedway is fixed to be connected
It connects in the front end of experimental vehicle, vacuum plant is fixedly connected on the rear end of experimental vehicle, and liquid nitrogen feedway and vacuum plant are equal
It is connected to experimental vehicle, manual operation device is fixedly connected on the left end of experimental vehicle, and low temperature shooting recording equipment clearance fit exists
The upper end of experimental vehicle, manual operation device and low temperature shooting recording equipment are arranged in experimental vehicle.
As optimization of the invention further, a kind of electron cryo-microscopy can operational observations recording device, the liquid
Nitrogen feedway includes that Dewar bottle, liquid nitrogen pump, right liquid nitrogen pipes and left liquid nitrogen pipes, liquid nitrogen pump are connected to Dewar bottle by bellows
Inside, liquid nitrogen pump is connected to right liquid nitrogen pipes and left liquid nitrogen pipes, right liquid nitrogen pipes and left liquid nitrogen pipes and is fixedly connected in reality
It tests on carrier.
As optimization of the invention further, a kind of electron cryo-microscopy can operational observations recording device, described is true
Empty device includes vacuum pump, vacuum manifold road, a right vacuum pipe, right two vacuum pipes, the second from left vacuum pipe and the first from left vacuum
Pipeline, a right vacuum pipe, right two vacuum pipes, the second from left vacuum pipe are connected to vacuum manifold road with the first from left vacuum pipe, very
The rear end of empty total pipeline is connected to vacuum pump.
As optimization of the invention further, a kind of electron cryo-microscopy can operational observations recording device, the reality
Testing carrier includes outermost shell, upper end cover, two observation panels, the first from left circular hole, the second from left circular hole, right two circular holes, right a circular hole, center
Circular hole, left pipeline, vacuum insulating region, center housing, upper spacer, the left circular hole of center shell, the right circular hole in center, most intrinsic fog air bound are exhausted
Shell, liquid nitrogen flow slot and observation operating area, upper end cover are fixedly connected on the upper end of outermost shell and center housing, two sights
Cha Kou is arranged on upper end cover, and the middle-end of upper end cover, the first from left circular hole, the second from left circular hole, right two circular holes, the right side are arranged in center hole
One circular hole is successively set on the rear end of outermost shell from left to right, and left pipeline is fixedly connected on the left end of outermost shell, vacuum every
Exhausted region is arranged between outermost shell and center housing, and left pipeline is connected to vacuum insulating region, a right vacuum pipe and the first from left
Vacuum pipe is respectively fixedly connected in a right circular hole and the first from left circular hole, and a right vacuum pipe is connected to very with the first from left vacuum pipe
Empty shallow trench isolation, the lower end of center housing are fixedly connected on the lower end of the inner wall of outermost shell, and liquid nitrogen flow slot is centrally disposed
The lower end of inner walls, the right circular hole of the left circular hole of center shell and center are arranged at the rear end of center housing, and the second from left vacuum pipe is solid
Surely it is connected in the left circular hole of center shell and the second from left circular hole, right two vacuum pipes are fixedly connected on and the right circular hole in center and right two circular holes
Interior, the second from left vacuum pipe is connected to the inside of center housing with right two vacuum pipes, and the most exhausted shell of intrinsic fog air bound is fixedly connected on
In liquid nitrogen flow slot, upper spacer is fixedly connected between the most outer wall of the exhausted shell of intrinsic fog air bound and the inner wall of center housing, observation
Operating area is arranged in the most exhausted shell of intrinsic fog air bound, center hole connection observation operating area, center housing and most interior fog
The left end of isolation shell is respectively arranged with circular hole I and circular hole II.
As optimization of the invention further, a kind of electron cryo-microscopy can operational observations recording device, it is described most
The front end of outer housing, center housing and the most exhausted shell of intrinsic fog air bound is respectively provided with there are two circular hole, and right liquid nitrogen pipes are fixedly connected on
In circular hole on the right side of outermost shell, center housing and the most exhausted shell of intrinsic fog air bound, left liquid nitrogen pipes be fixedly connected on outermost shell,
In circular hole on the left of center housing and the most exhausted shell of intrinsic fog air bound, right liquid nitrogen pipes are connected to liquid nitrogen flow with left liquid nitrogen pipes
Slot.
As optimization of the invention further, a kind of electron cryo-microscopy can operational observations recording device, the hand
Dynamic operating device includes fixed -piping, sealing baffle, outer electromagnetism ring, left end cap, seal rubber, adjustment operation pipe, left and right displacement
Pipe, internal magnetic ring, connecting rod, two strong magnetic are fast, sphere, copper mesh connecting rod, copper mesh are clapped, wire guide and electromagnetic block, fixed -piping are solid
Surely it is connected in the circular hole I of center housing and the circular hole II of the most exhausted shell of intrinsic fog air bound, fixed -piping connection observation operating area,
Fixed -piping inner wall is provided with sliding slot, and sealing baffle is fixedly connected on fixed -piping, and sealing baffle is by being threadedly engaged connection
In the left end of left pipeline, outer electromagnetism ring is slidably connected on fixed -piping, and the left end of sealing baffle, left end is arranged in outer electromagnetism ring
Lid is fixedly connected on the left end of fixed -piping, and seal rubber is fixedly connected in left end cap, and adjustment operation pipe is fixedly connected on close
It seals in rubber, the upper and lower ends of left and right displacement pipe are provided with sliding block, and the inner wall of left and right displacement pipe is provided with ball groove, and internal magnetic ring is solid
Surely it is connected in the sliding block of left and right displacement pipe, sphere is fixedly connected in connecting rod, sphere is rotatably connected on the ball of left and right displacement pipe
In slot, two strong magnetic is fixedly connected with fastly in the left end of connecting rod, and copper mesh connecting rod is fixedly connected on the right end of connecting rod, copper mesh
Clap adjusted by being threadedly engaged the right end for being connected to copper mesh connecting rod the right end clearance fit of operation pipe two strong magnetic fastly it
Between, electromagnetic block setting is in adjustment operation pipe, and on adjustment operation pipe, wire guide is connected to the interior of adjustment operation pipe for wire guide setting
Portion.
As optimization of the invention further, a kind of electron cryo-microscopy can operational observations recording device, described is low
Temperature shooting recording equipment is fixedly connected in center hole, and low temperature shoots the lower end setting of recording equipment in copper mesh bat.
Using technical solution provided by the invention, compared with prior art, has the advantages that and supply dress for liquid nitrogen
Set and provide the environment of a relative vacuum with vacuum plant for freezing biological sample, guarantee freezing environment, at the same by vacuum every
Absolutely, guarantee experiment safety, manual operation device can in all directions multi-angle adjustment sample shooting angle, guarantee shooting quality,
It ensures the comprehensive of observation, while reducing liquid nitrogen in vaporescence to the influence of shooting.
Detailed description of the invention
Fig. 1 is whole structural schematic diagram one of the invention；
Fig. 2 is the structural schematic diagram of liquid nitrogen feedway of the invention；
Fig. 3 is the structural schematic diagram of vacuum plant of the invention；
Fig. 4 is the structural schematic diagram one of experimental vehicle of the invention；
Fig. 5 is the structural schematic diagram two of experimental vehicle of the invention；
Fig. 6 is the structural schematic diagram three of experimental vehicle of the invention；
Fig. 7 is the structural schematic diagram four of experimental vehicle of the invention；
Fig. 8 is the structural schematic diagram five of experimental vehicle of the invention；
Fig. 9 is the structural schematic diagram one of manual operation device of the invention；
Figure 10 is the structural schematic diagram two of manual operation device of the invention；
Figure 11 is the structural schematic diagram three of manual operation device of the invention；
Figure 12 is the structural schematic diagram four of manual operation device of the invention；
Figure 13 is the structural schematic diagram five of manual operation device of the invention.
In figure: liquid nitrogen feedway 1；Dewar bottle 1-1；Liquid nitrogen pump 1-2；Right liquid nitrogen pipes 1-3；Left liquid nitrogen pipes 1-4；Very
Empty device 2；Vacuum pump 2-1；Vacuum manifold road 2-2；One vacuum pipe 2-3 of the right side；Two vacuum pipe 2-4 of the right side；The second from left vacuum pipe
2-5；The first from left vacuum pipe 2-6；Experimental vehicle 3；Outermost shell 3-1；Upper end cover 3-2；Observation panel 3-3；The first from left circular hole 3-4；It is left
Two circular hole 3-5；Two circular hole 3-6 of the right side；One circular hole 3-7 of the right side；Center hole 3-8；Left pipeline 3-9；Vacuum insulating region 3-10；Center
Shell 3-11；Upper spacer 3-12；The left circular hole 3-13 of center shell；The right circular hole 3-14 in center；The exhausted shell 3-15 of most intrinsic fog air bound；Liquid nitrogen
Flowing groove 3-16；Observe operating area 3-17；Manual operation device 4；Fixed -piping 4-1；Sealing baffle 4-2；Outer electromagnetism ring 4-
3；Left end cap 4-4；Seal rubber 4-5；Adjust operation pipe 4-6；Left and right displacement pipe 4-7；Internal magnetic ring 4-8；Connecting rod 4-9；Qiang Ci
Fast 4-10；Sphere 4-11；Copper mesh connecting rod 4-12；Copper mesh claps 4-13；Wire guide 4-14；Electromagnetic block 4-15；Low temperature shooting record
Equipment 5.
Specific embodiment
The present invention is described in further detail below in conjunction with the accompanying drawings.
It is fixedly connected described in the present apparatus and refers to and be fixed by the modes such as weld, be screwed, in conjunction with different
Use environment, using different fixed forms, the rotation connection refers to by drying bearing on axis, is kept off by elasticity
Circle realizes the axial restraint of bearing, realizes that rotation, described being slidably connected refer to that the sliding by sliding block in sliding slot is connected
It connects, it is by sealing ring or O-ring at required sealing that described hingedly referring to, which carries out movable connection by pin shaft and short axle,
Realize sealing.
Specific embodiment 1:
As shown in Fig. 1~Figure 13, a kind of electron cryo-microscopy can operational observations recording device, including liquid nitrogen feedway 1,
Vacuum plant 2, experimental vehicle 3, manual operation device 4 and low temperature shoot recording equipment 5, and the liquid nitrogen feedway 1 is fixed
It is connected to the front end of experimental vehicle 3, vacuum plant 2 is fixedly connected on the rear end of experimental vehicle 3, liquid nitrogen feedway 1 and vacuum
Device 2 is connected to experimental vehicle 3, and manual operation device 4 is fixedly connected on the left end of experimental vehicle 3, and low temperature shoots recording equipment 5
Clearance fit is arranged at experimental vehicle 3 in the upper end of experimental vehicle 3, manual operation device 4 and low temperature shooting recording equipment 5
It is interior.Low temperature shooting recording equipment 5 is chosen for the prior art, it is ensured that is shot in the environment of extremely low temperature；Liquid nitrogen supply
Device 1 and vacuum plant 2 make the vacuum environment that an extremely low temperature is ensured in experimental vehicle 3, make the sample on manual operation device 4
Quick freeze avoids having other remaining molecules on sample, and moving operating device 4 by Omnibearing operating makes low temperature shooting record
The shooting angle of sample is more fully on 5 pairs of operation manual operation devices 4 of equipment.
Specific embodiment 2:
As shown in Fig. 1~Figure 13, present embodiment is described further embodiment one, the liquid nitrogen feedway
1 includes that Dewar bottle 1-1, liquid nitrogen pump 1-2, right liquid nitrogen pipes 1-3 and left liquid nitrogen pipes 1-4, liquid nitrogen pump 1-2 are connected to by bellows
The inside of Dewar bottle 1-1, liquid nitrogen pump 1-2 are connected to right liquid nitrogen pipes 1-3 and left liquid nitrogen pipes 1-4, right liquid nitrogen pipes 1-3 and left liquid
Nitrogen pipeline 1-4 is fixedly connected on experimental vehicle 3.
Specific embodiment 3:
As shown in Fig. 1~Figure 13, present embodiment is described further embodiment two, and the vacuum plant 2 wraps
Include vacuum pump 2-1, vacuum manifold road 2-2, one vacuum pipe 2-3 of the right side, the right side two vacuum pipe 2-4, the second from left vacuum pipe 2-5 and a left side
One vacuum pipe 2-6, one vacuum pipe 2-3 of the right side, the right side two vacuum pipe 2-4, the second from left vacuum pipe 2-5 and the first from left vacuum pipe 2-
6 are connected to vacuum manifold road 2-2, and the rear end of vacuum manifold road 2-2 is connected to vacuum pump 2-1.Between the pipeline and pipeline
It is to pass through welded connecting.
Specific embodiment 4:
As shown in Fig. 1~Figure 13, present embodiment is described further embodiment three, and the experimental vehicle 3 wraps
Include outermost shell 3-1, upper end cover 3-2, two observation panel 3-3, the first from left circular hole 3-4, the second from left circular hole 3-5, the right side two circular hole 3-6, the right side
One circular hole 3-7, center hole 3-8, left pipeline 3-9, vacuum insulating region 3-10, center housing 3-11, upper spacer 3-12, center
The left circular hole 3-13 of shell, the right circular hole 3-14 in center, the exhausted shell 3-15 of most intrinsic fog air bound, liquid nitrogen flow slot 3-16 and observation operating area
3-17, upper end cover 3-2 are fixedly connected on the upper end of outermost shell 3-1 and center housing 3-11, and two observation panel 3-3 are arranged at
On upper end cover 3-2, the middle-end of upper end cover 3-2, the first from left circular hole 3-4, the second from left circular hole 3-5, right two circular holes are arranged in center hole 3-8
3-6, one circular hole 3-7 of the right side are successively set on the rear end of outermost shell 3-1 from left to right, and left pipeline 3-9 is fixedly connected on outermost shell
The left end of body 3-1, vacuum insulating region 3-10 are arranged between outermost shell 3-1 and center housing 3-11, left pipeline 3-9 connection
Vacuum insulating region 3-10, the right side one vacuum pipe 2-3 and the first from left vacuum pipe 2-6 be respectively fixedly connected in one circular hole 3-7 of the right side and
In the first from left circular hole 3-4, one vacuum pipe 2-3 of the right side is connected to vacuum insulating region 3-10, center housing with the first from left vacuum pipe 2-6
The lower end of 3-11 is fixedly connected on the lower end of the inner wall of outermost shell 3-1, the centrally disposed shell 3-11 of liquid nitrogen flow slot 3-16
The lower end of inner wall, the left circular hole 3-13 of center shell and the right circular hole 3-14 in center are arranged at the rear end of center housing 3-11, and the second from left is true
Vacant duct 2-5 is fixedly connected in center shell left circular hole 3-13 and the second from left circular hole 3-5, and two vacuum pipe 2-4 of the right side are fixedly connected on
In the right circular hole 3-14 in center and two circular hole 3-6 of the right side, the second from left vacuum pipe 2-5 is connected to center shell with two vacuum pipe 2-4 of the right side
The exhausted shell 3-15 in the inside of body 3-11, most intrinsic fog air bound is fixedly connected in liquid nitrogen flow slot 3-16, and upper spacer 3-12 is fixed to be connected
It connects between the outer wall of the exhausted shell 3-15 of most intrinsic fog air bound and the inner wall of center housing 3-11, observation operating area 3-17 setting exists
In the exhausted shell 3-15 of most intrinsic fog air bound, center hole 3-8 connection observation operating area 3-17, center housing 3-11 and most interior fog
The left end of isolation shell 3-15 is respectively arranged with circular hole I and circular hole II.Macromolecule is provided on the exhausted shell 3-15 of most intrinsic fog air bound
One-way ventilating film；Low temperature resistant acrylic glass is provided in two observation panel 3-3.
Specific embodiment 5:
As shown in Fig. 1~Figure 13, present embodiment is described further embodiment four, the outermost shell 3-1,
The front end of the exhausted shell 3-15 of center housing 3-11 and most intrinsic fog air bound is respectively provided with there are two circular hole, and right liquid nitrogen pipes 1-3 is fixed to be connected
It connects in the circular hole on the right side of the exhausted shell 3-15 of outermost shell 3-1, center housing 3-11 and most intrinsic fog air bound, left liquid nitrogen pipes 1-4
It is fixedly connected in the circular hole on the left of outermost shell 3-1, center housing 3-11 and the exhausted shell 3-15 of most intrinsic fog air bound, right liquid nitrogen pipe
Road 1-3 is connected to liquid nitrogen flow slot 3-16 with left liquid nitrogen pipes 1-4.
Specific embodiment 6:
As shown in Fig. 1~Figure 13, present embodiment is described further embodiment five, the manual operation device
4 include fixed -piping 4-1, sealing baffle 4-2, outer electromagnetism ring 4-3, left end cap 4-4, seal rubber 4-5, adjustment operation pipe 4-6,
Left and right displacement pipe 4-7, internal magnetic ring 4-8, connecting rod 4-9, two the fast 4-10 of strong magnetic, sphere 4-11, copper mesh connecting rod 4-12, copper mesh
Clap 4-13, wire guide 4-14 and electromagnetic block 4-15, fixed -piping 4-1 are fixedly connected on the circular hole I of center housing 3-11 and most interior
Fog completely cuts off in the circular hole II of shell 3-15, and fixed -piping 4-1 connection observation operating area 3-17, fixed -piping 4-1 inner wall is set
It is equipped with sliding slot, sealing baffle 4-2 is fixedly connected on fixed -piping 4-1, and sealing baffle 4-2 is connected to left pipe by being threadedly engaged
The left end of road 3-9, outer electromagnetism ring 4-3 are slidably connected on fixed -piping 4-1, and outer electromagnetism ring 4-3 is arranged in sealing baffle 4-2's
Left end, left end cap 4-4 are fixedly connected on the left end of fixed -piping 4-1, and seal rubber 4-5 is fixedly connected in left end cap 4-4, adjust
Whole operation pipe 4-6 is fixedly connected in seal rubber 4-5, and the upper and lower ends of left and right displacement pipe 4-7 are provided with sliding block, left and right position
The inner wall for moving pipe 4-7 is provided with ball groove, and internal magnetic ring 4-8 is fixedly connected in the sliding block of left and right displacement pipe 4-7, on connecting rod 4-9
It is fixedly connected with sphere 4-11, sphere 4-11 is rotatably connected in the ball groove of left and right displacement pipe 4-7, and two fast 4-10 of strong magnetic are fixed
It is connected to the left end of connecting rod 4-9, copper mesh connecting rod 4-12 is fixedly connected on the right end of connecting rod 4-9, and copper mesh is clapped 4-13 and passed through
It is threadedly engaged the right end for being connected to copper mesh connecting rod 4-12, adjusts the right end clearance fit of operation pipe 4-6 in two fast 4- of strong magnetic
Between 10, electromagnetic block 4-15 setting is in adjustment operation pipe 4-6, and wire guide 4-14 setting is on adjustment operation pipe 4-6, wire guide
The inside of 4-14 connection adjustment operation pipe 4-6.
Specific embodiment 7:
As shown in Fig. 1~Figure 13, present embodiment is described further embodiment six, and the low temperature shoots record
Equipment 5 is fixedly connected in center hole 3-8, and the lower end setting that low temperature shoots recording equipment 5 is clapped on 4-13 in copper mesh.
The operation principle of the present invention is that: sample is placed on copper mesh and is clapped on the copper mesh of 4-13, the copper mesh for having sample is clapped into 4-
13 pass through the sealing of sealing and seal rubber 4-5 of the left end cap 4-4 on left pipeline 3-9, so that the copper mesh for having sample is clapped 4-13 and put
It sets in observation operating area 3-17, by opening vacuum pump 2-1, the right side one vacuum pipe 2-3 and the first from left vacuum pipe 2-6 will be true
It is evacuated in empty shallow trench isolation 3-10, ensures low temperature environment, avoid frostbite operator of leaking, pass through two vacuum pipe 2- of the right side
Environment in center housing 3-11 is evacuated by 4 and the second from left vacuum pipe 2-5, by the exhausted shell 3-15 of most intrinsic fog air bound
The characteristic of macromolecule one-way ventilating film makes to observe vacuum in operating area 3-17, provides the vacuum environment of freezing；Open liquid nitrogen
1-2 is pumped, liquid nitrogen is flowed in liquid nitrogen flow slot 3-16 by right liquid nitrogen pipes 1-3 and left liquid nitrogen pipes 1-4, passes through the vapour of liquid nitrogen
Change, make to observe rapid cooling in operating area 3-17 and center housing 3-11, the sample for clapping copper mesh on 4-13 is quick-frozen, passes through two
In a observation panel 3-3 on low temperature resistant acrylic glass observed, and by low temperature shoot recording equipment 5 shoot, be
It ensures comprehensive shooting angle, dispatch from foreign news agency magnet ring 4-3 can be made to connect electricity, by moving left and right outer electromagnetism ring 4-3, electromagnetism ring 4-3
Absorption internal magnetic ring 4-8 and then left and right displacement pipe 4-7 is driven to move left and right, and then drives connecting rod 4-9, sphere 4-11, two strong
The fast 4-10 of magnetic, copper mesh connecting rod 4-12 and copper mesh clap 4-13 and move left and right, and then make sample or so adjustment, while passing through electromagnetic block
4-15 connects electricity, operates connecting rod 4-9, sphere 4-11, copper mesh connecting rod 4-12 and copper by adsorbing the fast 4-10 of two strong magnetic
Net claps 4-13, can be manually rotated displacement of the adjustment operation pipe 4-6 on seal rubber 4-5, so make connecting rod 4-9, sphere 4-11,
Copper mesh connecting rod 4-12 and copper mesh clap 4-13 rotation, and then adjust sample rotates, are shot.
Above description is not limitation of the present invention, and the present invention is also not limited to the example above, the art it is common
The variations, modifications, additions or substitutions that technical staff is made within the essential scope of the present invention also belong to protection model of the invention
It encloses.
Claims (7)
1. a kind of electron cryo-microscopy can operational observations recording device, including liquid nitrogen feedway (1), vacuum plant (2), experiment
Carrier (3), manual operation device (4) and low temperature shooting recording equipment (5), it is characterised in that: the liquid nitrogen feedway (1)
It is fixedly connected on the front end of experimental vehicle (3), vacuum plant (2) is fixedly connected on the rear end of experimental vehicle (3), liquid nitrogen supply dress
It sets (1) to be connected to experimental vehicle (3) with vacuum plant (2), manual operation device (4) is fixedly connected on a left side for experimental vehicle (3)
End, low temperature shoot recording equipment (5) clearance fit in the upper end of experimental vehicle (3), manual operation device (4) and low temperature shooting note
Recording apparatus (5) is arranged in experimental vehicle (3).
2. a kind of electron cryo-microscopy according to claim 1 can operational observations recording device, it is characterised in that: it is described
Liquid nitrogen feedway (1) includes Dewar bottle (1-1), liquid nitrogen pump (1-2), right liquid nitrogen pipes (1-3) and left liquid nitrogen pipes (1-4),
Liquid nitrogen pump (1-2) is connected to the inside of Dewar bottle (1-1) by bellows, and liquid nitrogen pump (1-2) is connected to right liquid nitrogen pipes (1-3) and a left side
Liquid nitrogen pipes (1-4), right liquid nitrogen pipes (1-3) and left liquid nitrogen pipes (1-4) are fixedly connected on experimental vehicle (3).
3. a kind of electron cryo-microscopy according to claim 2 can operational observations recording device, it is characterised in that: it is described
Vacuum plant (2) includes vacuum pump (2-1), vacuum manifold road (2-2), a right vacuum pipe (2-3), two vacuum pipe (2- of the right side
4), the second from left vacuum pipe (2-5) and the first from left vacuum pipe (2-6), a right vacuum pipe (2-3), right two vacuum pipes (2-4),
The second from left vacuum pipe (2-5) is connected to vacuum manifold road (2-2) with the first from left vacuum pipe (2-6), after vacuum manifold road (2-2)
End connection vacuum pump (2-1).
4. a kind of electron cryo-microscopy according to claim 3 can operational observations recording device, it is characterised in that: it is described
Experimental vehicle (3) includes outermost shell (3-1), upper end cover (3-2), two observation panels (3-3), the first from left circular hole (3-4), the second from left circle
Hole (3-5), right two circular holes (3-6), a right circular hole (3-7), center hole (3-8), left pipeline (3-9), vacuum insulating region (3-
10), center housing (3-11), upper spacer (3-12), the left circular hole of center shell (3-13), the right circular hole in center (3-14), most interior fog
Completely cut off shell (3-15), liquid nitrogen flow slot (3-16) and observation operating area (3-17), upper end cover (3-2) is fixedly connected on outermost
The upper end of shell (3-1) and center housing (3-11), two observation panels (3-3) are arranged on upper end cover (3-2), center hole
The middle-end in upper end cover (3-2), the first from left circular hole (3-4), the second from left circular hole (3-5), right two circular holes (3-6), one circle of the right side is arranged in (3-8)
Hole (3-7) is successively set on the rear end of outermost shell (3-1) from left to right, and left pipeline (3-9) is fixedly connected on outermost shell (3-
1) left end, vacuum insulating region (3-10) are arranged between outermost shell (3-1) and center housing (3-11), left pipeline (3-
9) it is connected to vacuum insulating region (3-10), a right vacuum pipe (2-3) and the first from left vacuum pipe (2-6) are respectively fixedly connected on the right side
In one circular hole (3-7) and the first from left circular hole (3-4), a right vacuum pipe (2-3) and the first from left vacuum pipe (2-6) be connected to vacuum every
Exhausted region (3-10), the lower end of center housing (3-11) is fixedly connected on the lower end of the inner wall of outermost shell (3-1), liquid nitrogen flow
The lower end of slot (3-16) centrally disposed shell (3-11) inner wall, the right circular hole of the left circular hole of center shell (3-13) and center (3-14) are equal
The rear end of centrally disposed shell (3-11), the second from left vacuum pipe (2-5) are fixedly connected on the left circular hole of center shell (3-13) and a left side
In two circular holes (3-5), right two vacuum pipes (2-4) be fixedly connected on it is interior with the right circular hole in center (3-14) and right two circular holes (3-6),
The second from left vacuum pipe (2-5) is connected to the inside of center housing (3-11), the most exhausted shell of intrinsic fog air bound with right two vacuum pipes (2-4)
Body (3-15) is fixedly connected in liquid nitrogen flow slot (3-16), and upper spacer (3-12) is fixedly connected on the most exhausted shell of intrinsic fog air bound
Between the inner wall of the outer wall and center housing (3-11) of (3-15), observation operating area (3-17) is arranged in the most exhausted shell of intrinsic fog air bound
In body (3-15), center hole (3-8) connection observation operating area (3-17), center housing (3-11) and the most exhausted shell of intrinsic fog air bound
The left end of body (3-15) is respectively arranged with circular hole I and circular hole II.
5. a kind of electron cryo-microscopy according to claim 4 can operational observations recording device, it is characterised in that: it is described
The front end of outermost shell (3-1), center housing (3-11) and the most exhausted shell of intrinsic fog air bound (3-15) is respectively provided with there are two circular hole, right
It is right that liquid nitrogen pipes (1-3) are fixedly connected on outermost shell (3-1), center housing (3-11) and the most exhausted shell of intrinsic fog air bound (3-15)
In the circular hole of side, left liquid nitrogen pipes (1-4) are fixedly connected on outermost shell (3-1), center housing (3-11) and most intrinsic fog air bound
In circular hole on the left of exhausted shell (3-15), right liquid nitrogen pipes (1-3) are connected to liquid nitrogen flow slot (3- with left liquid nitrogen pipes (1-4)
16)。
6. a kind of electron cryo-microscopy according to claim 5 can operational observations recording device, it is characterised in that: it is described
Manual operation device (4) includes fixed -piping (4-1), sealing baffle (4-2), outer electromagnetism ring (4-3), left end cap (4-4), sealing
Rubber (4-5), adjustment operation pipe (4-6), left and right displacement pipe (4-7), internal magnetic ring (4-8), connecting rod (4-9), two strong magnetic are fast
(4-10), sphere (4-11), copper mesh connecting rod (4-12), copper mesh clap (4-13), wire guide (4-14) and electromagnetic block (4-15), Gu
Determine pipeline (4-1) to be fixedly connected in the circular hole I of center housing (3-11) and the circular hole II of the most exhausted shell of intrinsic fog air bound (3-15),
Fixed -piping (4-1) connection observation operating area (3-17), fixed -piping (4-1) inner wall are provided with sliding slot, sealing baffle (4-2)
It is fixedly connected on the left end of fixed -piping (4-1), sealing baffle (4-2) is by being threadedly engaged the left side for being connected to left pipeline (3-9)
End, outer electromagnetism ring (4-3) are slidably connected on fixed -piping (4-1), and outer electromagnetism ring (4-3) is arranged in sealing baffle (4-2)
Left end, left end cap (4-4) are fixedly connected on the left end of fixed -piping (4-1), and seal rubber (4-5) is fixedly connected on left end cap
In (4-4), adjustment operation pipe (4-6) is fixedly connected in seal rubber (4-5), and the upper and lower ends of left and right displacement pipe (4-7) are equal
It is provided with sliding block, the inner wall of left and right displacement pipe (4-7) is provided with ball groove, and internal magnetic ring (4-8) is fixedly connected on left and right displacement pipe (4-
7) it in sliding block, is fixedly connected in connecting rod (4-9) sphere (4-11), sphere (4-11) clearance fit is in left and right displacement pipe (4-
7) in ball groove, two strong magnetic fast (4-10) is fixedly connected in the left end of connecting rod (4-9), and copper mesh connecting rod (4-12) is fixed
It is connected to the right end of connecting rod (4-9), copper mesh bat (4-13), which passes through, is threadedly engaged the right end for being connected to copper mesh connecting rod (4-12),
The right end clearance fit of operation pipe (4-6) is adjusted between two strong magnetic fast (4-10), electromagnetic block (4-15) setting is grasped in adjustment
It manages in (4-6), wire guide (4-14) setting is in adjustment operation pipe (4-6), wire guide (4-14) connection adjustment operation pipe (4-
6) inside.
7. a kind of electron cryo-microscopy according to claim 6 can operational observations recording device, it is characterised in that: it is described
Low temperature shooting recording equipment (5) is fixedly connected in center hole (3-8), and the lower end setting that low temperature shoots recording equipment (5) exists
Copper mesh is clapped on (4-13).
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110542259A (en) * | 2019-08-09 | 2019-12-06 | 河南中镜科仪科技有限公司 | Quick freezing device |
| CN111175329A (en) * | 2020-01-16 | 2020-05-19 | 中国石油大学(北京) | In-situ observation method for microstructure of liquid nitrogen frozen rock |
| CN113655077A (en) * | 2021-08-13 | 2021-11-16 | 中国科学院化学研究所 | Liquid nitrogen automatic filling and data acquisition linkage device and method for cryoelectron microscope |
Citations (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5147611A (en) * | 1989-07-24 | 1992-09-15 | Union Oil Company Of California | Optical and pyrolyzate analyzer apparatus |
| CN1363893A (en) * | 2001-10-16 | 2002-08-14 | 甘肃工业大学 | Real-time acquisition system of low-temp metallographer for computer |
| US20060249687A1 (en) * | 2005-05-09 | 2006-11-09 | Bing-Huan Lee | Device for operating gas in vacuum or low-pressure environment and for observation of the operation |
| CN101231249A (en) * | 2008-02-26 | 2008-07-30 | 东北大学 | Vacuum low temperature microscopic visualizer |
| CN101354365A (en) * | 2008-04-02 | 2009-01-28 | 中国科学院大连化学物理研究所 | Adiabatic calorimeter and heat measuring system |
| EP2626885A1 (en) * | 2012-02-13 | 2013-08-14 | FEI Company | Forming a vitrified sample for an electron microscopy |
| CN103278308A (en) * | 2013-04-23 | 2013-09-04 | 浙江大学 | Visualization experiment device for deep cryogenic two-phase countercurrent process |
| CN104181105A (en) * | 2013-05-23 | 2014-12-03 | 中国科学院大连化学物理研究所 | Sample tank used for observation of liquid oxygen fluorescence spectrum |
| CN104634737A (en) * | 2013-11-15 | 2015-05-20 | 中国科学院生物物理研究所 | Manual ultralow-temperature sample stage for iPALM (interferometric photoactivated localization microscopy) microscope |
| US20160030941A1 (en) * | 2014-07-29 | 2016-02-04 | Leica Mikrosysteme Gmbh | Manipulation holder for cryomicroscopy |
| CN106370692A (en) * | 2016-08-27 | 2017-02-01 | 上海交通大学 | Visual observation device for dissolution and separation of trace soluble impurities in cryogenic liquid |
| CN106568794A (en) * | 2016-11-04 | 2017-04-19 | 上海交通大学 | Low temperature refrigerating machine-based visual experiment observation apparatus of controlled liquefaction and solidification process of fluid |
| WO2017098574A1 (en) * | 2015-12-08 | 2017-06-15 | 株式会社日立ハイテクノロジーズ | Anti-contamination trap, control method therefor, and charged particle beam device |
| US20170213694A1 (en) * | 2014-07-29 | 2017-07-27 | Leica Mikrosysteme Gmbh | Loading station for transferring frozen samples at low temperatures |
-
2018
- 2018-06-15 CN CN201810622825.5A patent/CN109030519B/en active Active
Patent Citations (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5147611A (en) * | 1989-07-24 | 1992-09-15 | Union Oil Company Of California | Optical and pyrolyzate analyzer apparatus |
| CN1363893A (en) * | 2001-10-16 | 2002-08-14 | 甘肃工业大学 | Real-time acquisition system of low-temp metallographer for computer |
| US20060249687A1 (en) * | 2005-05-09 | 2006-11-09 | Bing-Huan Lee | Device for operating gas in vacuum or low-pressure environment and for observation of the operation |
| CN101231249A (en) * | 2008-02-26 | 2008-07-30 | 东北大学 | Vacuum low temperature microscopic visualizer |
| CN101354365A (en) * | 2008-04-02 | 2009-01-28 | 中国科学院大连化学物理研究所 | Adiabatic calorimeter and heat measuring system |
| EP2626885A1 (en) * | 2012-02-13 | 2013-08-14 | FEI Company | Forming a vitrified sample for an electron microscopy |
| CN103278308A (en) * | 2013-04-23 | 2013-09-04 | 浙江大学 | Visualization experiment device for deep cryogenic two-phase countercurrent process |
| CN104181105A (en) * | 2013-05-23 | 2014-12-03 | 中国科学院大连化学物理研究所 | Sample tank used for observation of liquid oxygen fluorescence spectrum |
| CN104634737A (en) * | 2013-11-15 | 2015-05-20 | 中国科学院生物物理研究所 | Manual ultralow-temperature sample stage for iPALM (interferometric photoactivated localization microscopy) microscope |
| US20160030941A1 (en) * | 2014-07-29 | 2016-02-04 | Leica Mikrosysteme Gmbh | Manipulation holder for cryomicroscopy |
| US20170213694A1 (en) * | 2014-07-29 | 2017-07-27 | Leica Mikrosysteme Gmbh | Loading station for transferring frozen samples at low temperatures |
| WO2017098574A1 (en) * | 2015-12-08 | 2017-06-15 | 株式会社日立ハイテクノロジーズ | Anti-contamination trap, control method therefor, and charged particle beam device |
| CN106370692A (en) * | 2016-08-27 | 2017-02-01 | 上海交通大学 | Visual observation device for dissolution and separation of trace soluble impurities in cryogenic liquid |
| CN106568794A (en) * | 2016-11-04 | 2017-04-19 | 上海交通大学 | Low temperature refrigerating machine-based visual experiment observation apparatus of controlled liquefaction and solidification process of fluid |
Non-Patent Citations (2)
| Title |
|---|
| 张绍志: "低温显微镜的研制", 《低温工程》 * |
| 王俊听: "低温扫描隧道显微镜系统研制及层状材料缺陷研究", 《中国博士学位论文全文数据库 工程科技Ⅱ辑》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110542259A (en) * | 2019-08-09 | 2019-12-06 | 河南中镜科仪科技有限公司 | Quick freezing device |
| CN111175329A (en) * | 2020-01-16 | 2020-05-19 | 中国石油大学(北京) | In-situ observation method for microstructure of liquid nitrogen frozen rock |
| CN113655077A (en) * | 2021-08-13 | 2021-11-16 | 中国科学院化学研究所 | Liquid nitrogen automatic filling and data acquisition linkage device and method for cryoelectron microscope |
| CN113655077B (en) * | 2021-08-13 | 2022-11-22 | 中国科学院化学研究所 | Liquid nitrogen automatic filling and data acquisition linkage device and method for cryoelectron microscope |
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