CN109010397A - The Chinese materia medica preparation and preparation method thereof for treating pain - Google Patents
The Chinese materia medica preparation and preparation method thereof for treating pain Download PDFInfo
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Abstract
The invention discloses the Chinese materia medica preparations for the treatment of pain, the preparation method of the traditional Chinese medicine preparation comprises the following steps of: CAULIS SCHEFFLERAE KWANGSIENSIS medicinal material, after crushed, it is infiltrated with 5% sodium bicarbonate solution, 30-40% ethyl alcohol heating and refluxing extraction is used again, filtration, filtrate stand 24-48 hours in 0-4 DEG C of freezer, filter, medicinal extract is obtained after decompression filtrate recycling ethanol, add water, mix, being adjusted with acid solution ph is 3-4, the butanol solution extraction being saturated with water again, n-butanol is recovered under reduced pressure in extract liquor, obtains medicinal extract, adds 50% ethyl alcohol, glycerol, osmanthus nitrogen
Description
Technical field
Extraction separation and applied technical field the present invention relates to Chinese medicine, and in particular to treat pain Chinese materia medica preparation and its
Preparation method.
Background technique
Pain is clinical common sympton, and the complication of many diseases all with pain, is brought great pain to patients.Mesh
Preceding clinically common anodyne refer to can partially or completely medicine for relieving pain, have non-steroidal anti-inflammatory drugs and central analgesic
Two class of medicine.Common anodyne has aspirin, somedon, paracetamol, phenylbutazone, rofecoxib etc..But it is used for a long time, stomach and intestine
Road reaction and renal toxicity increase, erious adverse reaction, are unfavorable for the treatment and recovery of patient disease, and some anesthesia classes have habituation
Property, therefore it is unable to prolonged application.And Chinese Traditional Medicine significantly improves clinical symptoms and sign treating pain or have when complication,
Such as rhizoma corydalis, Radix Notoginseng, with adverse reaction is few and the advantages such as light.CAULIS SCHEFFLERAE KWANGSIENSIS is in treatment sciatica, trigeminal neuralgia etc.
The special efficacy of stubborn and stupid pain causes people and more and more pays close attention to.
CAULIS SCHEFFLERAE KWANGSIENSIS is Chinese medicine simply with Guangxi national characters, records in " Guangxi Chinese herbal medicine ", is Araliaceae
The drying stem and branch with leaf or stem branch of Radix Schfflerae kwangisensis Schefllera kwangsiensis Merr.ex Li.Its nature and flavor slight bitter,
Puckery, temperature has wind-expelling pain-stopping, relaxing tendons and activating collaterals and other effects.It is mainly used for treating rheumatic arthritis, sciatica, trigeminal neuralgia
Pain and kidney, biliary tract pains and other diseases.CAULIS SCHEFFLERAE KWANGSIENSIS main product is distributed mainly on the counties such as Wuming, the Fusui in Guangxi in Guangxi." the Guangxi people
Race's medicine short course " in CAULIS SCHEFFLERAE KWANGSIENSIS the effect of and common method be described: root, stem are decocted in water for oral dose, treating rheumatic heart disease, premenstrual abdomen
Bitterly, it catches a cold；Wine clothes are rushed in decocting, control lumbago due to wind-wetness evil；Be decocted in water for oral dose simultaneous ablution, harnesses the river swollen；Deposited affected part is mashed, fracture is controlled；Mash deposited wound
Around mouthful, venomous snake bite is controlled.The research to CAULIS SCHEFFLERAE KWANGSIENSIS domestic at present is reported few, and Liu Xian etc. publishes thesis " the anti-inflammatory town of CAULIS SCHEFFLERAE KWANGSIENSIS
Pain acts on the screening of active component " in, preliminary screening is carried out to the petroleum ether, ethyl acetate, n-butanol portion of CAULIS SCHEFFLERAE KWANGSIENSIS, as a result
Show that ethyl acetate extract has more significant antalgic and inflammation relieving effect, but there are no further mentioning suitable for large-scale production
Take the report of purification process and application.Hu book equality develops CAULIS SCHEFFLERAE KWANGSIENSIS organic acid composition Study and ointment, using ion
Exchange chromatography and modern separation technology determine the optimum extraction process of total organic acids, and carry out pharmacological research, but extracting method
Complicated, high production cost, is not suitable for industrialized production.The present invention further study CAULIS SCHEFFLERAE KWANGSIENSIS relieve the pain pain effective active at
Point, the pain active component and suitable for the extraction of large-scale production of more effectively relieving the pain has been filtered out by optimizing method for extraction and purification
Method, and it is prepared into tincture, currently, there is not yet relevant report.
The disclosure of background above technology contents is only used for auxiliary and understands inventive concept and technical solution of the invention, not
The prior art for necessarily belonging to present patent application shows above content in the applying date of present patent application in no tangible proof
In the case where having disclosed, above-mentioned background technique should not be taken to the novelty and creativeness of evaluation the application.
Summary of the invention
The purpose of the present invention is to provide it is a kind of extracted from CAULIS SCHEFFLERAE KWANGSIENSIS have relieve the pain pain effect active component and its
Preparation method, and it is prepared into tincture, facilitate patient to use.
A kind of Chinese materia medica preparation for treating pain, the preparation method of the traditional Chinese medicine preparation comprises the following steps of: CAULIS SCHEFFLERAE KWANGSIENSIS medicinal material is used after crushed
5% sodium bicarbonate solution infiltration, then 30-40% ethyl alcohol heating and refluxing extraction is used, it filters, filtrate stands in 0-4 DEG C of freezer
It 24-48 hours, filters, medicinal extract is obtained after decompression filtrate recycling ethanol, adds water, mix, being adjusted with acid solution ph is 3-4, then is used
Water saturated butanol solution extraction, extract liquor are recovered under reduced pressure n-butanol, obtain medicinal extract, add 50% ethyl alcohol, glycerol, osmanthus nitrogen
Ketone, mix, filtration to get.
The Chinese materia medica preparation of above-described treatment pain, is made: CAULIS SCHEFFLERAE KWANGSIENSIS medicinal material of the supplementary material of following parts by weight
3000 parts, 50% ethyl alcohol 1000ml, glycerol 30ml, osmanthus nitrogenKetone 20ml.
The preparation method of the Chinese materia medica preparation of above-described treatment pain, comprising the following steps:
S1. 3000 parts of CAULIS SCHEFFLERAE KWANGSIENSIS medicinal material are taken, is crushed, is infiltrated with 5% sodium bicarbonate solution, then added with 30-40% ethyl alcohol
Circumfluence distillation, filtration, obtains primary filtrate；
S2. primary filtrate is stood 24-48 hours in 0-4 DEG C of freezer, filtered, medicinal extract is obtained after decompression filtrate recycling ethanol；
S3. water is added in medicinal extract, mixes, being adjusted with acid solution ph is 3-4；
S4. the butanol solution extraction being saturated with water, extract liquor are recovered under reduced pressure n-butanol, obtain CAULIS SCHEFFLERAE KWANGSIENSIS active component；
S5. 50% ethyl alcohol 1000ml, glycerol 30ml, osmanthus nitrogen are added in CAULIS SCHEFFLERAE KWANGSIENSIS active componentKetone 20ml is mixed, filter
Cross to get.
Further, CAULIS SCHEFFLERAE KWANGSIENSIS medicinal material is crushed in the step S1 into coarse powder.
Further, the dosage of 5% sodium bicarbonate solution is 1-1.2 times of medicinal material weight and measures in the step S1, soaks
Moistening the time is 20-30 minutes.
Further, heating and refluxing extraction number is 2-3 times in the step S1.
Further, 6-8 times that 30-40% amount of alcohol is CAULIS SCHEFFLERAE KWANGSIENSIS medicinal material weight is added in the step S1 to measure.
Further, amount of water is 4-6 times of medicinal extract weight and measures that the acid is hydrochloric acid or sulfuric acid in the step S3.
Further, the butanol solution being saturated with water in the step S4 extracts 2-3 times.
The Chinese materia medica preparation of the application of the Chinese materia medica preparation of the above treatment pain, the treatment pain is preventing or is treating pain
The pain external application such as the application of shape, especially rheumatic arthritis, sciatica, trigeminal neuralgia.
The beneficial effects of the present invention are:
1, the present invention is screened by modernization extraction separation method in conjunction with pharmacological evaluation, preferably obtains making with the pain that relieves the pain
With significant CAULIS SCHEFFLERAE KWANGSIENSIS active component, fumaric extract yield is up to 82% or more in the active component；Internal and body
Outer pharmacodynamic test shows to all have significant analgesia, anti-inflammatory effect, and CAULIS SCHEFFLERAE KWANGSIENSIS active component more reported in the literature activity it is strong,
Good drug efficacy.
2, the CAULIS SCHEFFLERAE KWANGSIENSIS active component with the pain effect that relieves the pain provided by the invention is prepared into tincture, facilitates patient's external application,
Energy minor betterment patient pain's state, facilitates clinical application.The preparation of the Chinese materia medica preparation for the treatment of pain provided by the invention simultaneously
Method, operation is simple, and the active component high income of extraction, strong operability, production cost is low, is suitble to industrial scale big
Production.
Specific embodiment
The invention will be further described combined with specific embodiments below, but does not limit the scope of the invention and apply
Range.CAULIS SCHEFFLERAE KWANGSIENSIS medicinal material used in the embodiment of the present invention is bought from Yulin medicinal material market, lot number 20171105, each embodiment
Medicinal material used is same batch.
One, the preparation of CAULIS SCHEFFLERAE KWANGSIENSIS active component and CAULIS SCHEFFLERAE KWANGSIENSIS tincture of the present invention
Embodiment 1
The preparation method of CAULIS SCHEFFLERAE KWANGSIENSIS active component, comprising the following steps:
S1. CAULIS SCHEFFLERAE KWANGSIENSIS medicinal material 5kg is taken, coarse powder is ground into, is infiltrated 20 minutes with 5% sodium bicarbonate solution 5kg, then with 6
Amount 30 ethyl alcohol heating and refluxing extraction 2 times again, 1 hour every time, filtration obtained primary filtrate；
S2. primary filtrate is stood 24 hours in 0-4 DEG C of freezer, filtered, medicinal extract is obtained after decompression filtrate recycling ethanol；
S3. 4 times of amount water are added in medicinal extract, mix, adjusting solution ph with hydrochloric acid solution is 3；
S4. the butanol solution being saturated with water extracts 2 times, and n-butanol is recovered under reduced pressure in extract liquor, dry, obtained extract
As CAULIS SCHEFFLERAE KWANGSIENSIS active component.
The preparation method of 1 CAULIS SCHEFFLERAE KWANGSIENSIS tincture of embodiment:
With the preparation method of the above CAULIS SCHEFFLERAE KWANGSIENSIS active component, the dosage of CAULIS SCHEFFLERAE KWANGSIENSIS medicinal material is 30kg, and being prepared into CAULIS SCHEFFLERAE KWANGSIENSIS has
Imitate position；50% ethyl alcohol 10000ml, glycerol 300ml, osmanthus nitrogen are added in CAULIS SCHEFFLERAE KWANGSIENSIS active componentKetone 200ml is mixed, filter
Cross to get.
Embodiment 2
The preparation method of CAULIS SCHEFFLERAE KWANGSIENSIS active component, comprising the following steps:
S1. CAULIS SCHEFFLERAE KWANGSIENSIS medicinal material 5kg is taken, coarse powder is ground into, is infiltrated 30 minutes with 5% sodium bicarbonate solution 6kg, then with 8
Amount 40% ethyl alcohol heating and refluxing extraction 3 times again, 2 hours every time, filtration obtained primary filtrate；
S2. primary filtrate is stood 48 hours in 0-4 DEG C of freezer, filtered, medicinal extract is obtained after decompression filtrate recycling ethanol；
S3. 6 times of amount water are added in medicinal extract, mix, adjusting solution ph with sulfuric acid solution is 4；
S4. the butanol solution being saturated with water extracts 3 times, and n-butanol is recovered under reduced pressure in extract liquor, dry, obtained extract
As CAULIS SCHEFFLERAE KWANGSIENSIS active component.
The preparation method of 2 CAULIS SCHEFFLERAE KWANGSIENSIS tincture of embodiment:
With the preparation method of the above CAULIS SCHEFFLERAE KWANGSIENSIS active component, the dosage of CAULIS SCHEFFLERAE KWANGSIENSIS medicinal material is 30kg, and being prepared into CAULIS SCHEFFLERAE KWANGSIENSIS has
Imitate position；50% ethyl alcohol 10000ml, glycerol 300ml, osmanthus nitrogen are added in CAULIS SCHEFFLERAE KWANGSIENSIS active componentKetone 200ml is mixed, filter
Cross to get.
Embodiment 3
The preparation method of CAULIS SCHEFFLERAE KWANGSIENSIS active component, comprising the following steps:
S1. CAULIS SCHEFFLERAE KWANGSIENSIS medicinal material 5kg is taken, coarse powder is ground into, is infiltrated 25 minutes with 5% sodium bicarbonate solution 5.5kg, then use
7 times amount 35% ethyl alcohol heating and refluxing extraction 2 times, 1.5 hours every time, filtration, obtain primary filtrate；
S2. primary filtrate is stood 32 hours in 0-4 DEG C of freezer, filtered, medicinal extract is obtained after decompression filtrate recycling ethanol；
S3. 5 times of amount water are added in medicinal extract, mix, adjusting solution ph with hydrochloric acid solution is 3.5；
S4. the butanol solution being saturated with water extracts 3 times, and n-butanol is recovered under reduced pressure in extract liquor, dry, obtained extract
As CAULIS SCHEFFLERAE KWANGSIENSIS active component.
The preparation method of 3 CAULIS SCHEFFLERAE KWANGSIENSIS tincture of embodiment:
With the preparation method of the above CAULIS SCHEFFLERAE KWANGSIENSIS active component, the dosage of CAULIS SCHEFFLERAE KWANGSIENSIS medicinal material is 30kg, and being prepared into CAULIS SCHEFFLERAE KWANGSIENSIS has
Imitate position；50% ethyl alcohol 10000ml, glycerol 300ml, osmanthus nitrogen are added in CAULIS SCHEFFLERAE KWANGSIENSIS active componentKetone 200ml is mixed, filter
Cross to get.
Embodiment 4
The preparation method of CAULIS SCHEFFLERAE KWANGSIENSIS active component, comprising the following steps:
S1. CAULIS SCHEFFLERAE KWANGSIENSIS medicinal material 5kg is taken, coarse powder is ground into, is infiltrated 20 minutes with 5% sodium bicarbonate solution 4kg, then with 8
Amount 40% ethyl alcohol heating and refluxing extraction 3 times again, 1 hour every time, filtration obtained primary filtrate；
S2. primary filtrate is stood 24 hours in 0-4 DEG C of freezer, filtered, medicinal extract is obtained after decompression filtrate recycling ethanol；
S3. 4 times of amount water are added in medicinal extract, mix, adjusting solution ph with sulfuric acid solution is 4；
S4. the butanol solution being saturated with water extracts 3 times, and n-butanol is recovered under reduced pressure in extract liquor, dry, obtained extract
As CAULIS SCHEFFLERAE KWANGSIENSIS active component.
The preparation method of 4 CAULIS SCHEFFLERAE KWANGSIENSIS tincture of embodiment:
With the preparation method of the above CAULIS SCHEFFLERAE KWANGSIENSIS active component, the dosage of CAULIS SCHEFFLERAE KWANGSIENSIS medicinal material is 30kg, and being prepared into CAULIS SCHEFFLERAE KWANGSIENSIS has
Imitate position；50% ethyl alcohol 10000ml, glycerol 300ml, osmanthus nitrogen are added in CAULIS SCHEFFLERAE KWANGSIENSIS active componentKetone 200ml is mixed, filter
Cross to get.
Two, the preparation of documents CAULIS SCHEFFLERAE KWANGSIENSIS anti-inflammatory and analgesic effect active component
Comparative example
CAULIS SCHEFFLERAE KWANGSIENSIS medicinal material 0.5kg is taken, coarse powder is ground into, is heated to reflux 3 times with 75% ethyl alcohol, 1 hour every time, is filtered, close
And extracting solution, ethyl alcohol is recycled, the powder that gets dry extract is dried in vacuo.With petroleum ether (60-90 DEG C) heating and refluxing extraction 3 times, 1 is small every time
When, petroleum ether extract is discarded, takes extraction residue, then with ethyl acetate heating and refluxing extraction 3 times, every time 1 hour, is filtered, is closed
And extracting solution, filtration, filtrate recycles ethyl acetate, and it is dry, obtain anti-inflammatory and analgesic effect active component comparative example sample.
Three, embodiment sample assay
1. chromatographic condition
Chromatographic column is Shimadzu VP-ODS column (250mm x 4.6mm): mobile phase is -0.2% phosphoric acid solution of methanol (5:95)；
Flow velocity is 1.OmL/min, and column temperature is room temperature；Detector is Japanese Shimadzu SPD-M10Avp UV detector, and the detection wave people are
210nm。
2. the configuration of solution
The preparation of reference substance solution: precision weighs that fumaric acid reference substance is appropriate, adds methanol that every 1mL is made containing 10 μ g's
Solution to get.
The preparation of sample solution: taking above-mentioned the present embodiment 1-4 active component, comparative example sample each 1g, accurately weighed,
It is set in 100mL conical flask respectively, adds 50% ethanol solution 50mL, weighed weight, heating water bath reflux 2h lets cool, adds 50% second
Alcohol supplies the weight of less loss, shakes up, 0.45 μ L miillpore filter filtration to get.
3. test result
The sample solution for taking above-described embodiment 1-4 active component, comparative example sample preparation to obtain, with fumaric acid
For the performance assessment criteria of assay, measurement result is shown in Table 1, and calculates extract yield.
1 embodiment sample assay result table of table
Above-mentioned test result shows that the fumaric acid content in 1-4 sample of the embodiment of the present invention is all larger than comparison and implements
Example group, extract yield height is 82% or more, high by 30% or more compared with comparative example group content, illustrates that the present invention is excellent through inventor
Change the extracting method of screening, the extraction of organic acid is more complete in CAULIS SCHEFFLERAE KWANGSIENSIS medicinal material, and extraction efficiency is high, and effective active composition contains
Amount is high.
Four, internal pharmacodynamics test
1. material
1.1 experiment reagents
Sample: respectively 3 active component of Example, prepare resulting CAULIS SCHEFFLERAE KWANGSIENSIS active component in comparative example, face use
The grinding of preceding plus water is configured to the suspension of various concentration, as test solution；Positive control: acetylsalicylic acid tablet is matched before use
It is spare at solution.
Reagent: rat, mouse, rabbit maintain feed (multifarious Jia Xing Biotechnology Co., Ltd of Wuhan City)；95% is edible
Grade alcohol is that chemistry is pure, remaining reagent is that analysis is pure.
1.2 laboratory apparatus
Electronic balance (0.1g-1000g, Zhejiang Kai Feng group company)；Digital display thermostat water bath (HH-6, the general day instrument in Changzhou
Device Manufacturing Co., Ltd)；Centrifuge (5430R, eppendorf desk type multifunctional refrigerated centrifuge)；Electrothermostat (DNP-
9052A, Xingtai Run Lian Science and Technology Development Co., Ltd.).
1.3 experimental animal
Cleaning grade Kunming mouse, 19~21g of weight；Cleaning grade Wistar rat, weight 150-200g；Regular grade is newly western
Blue rabbit, 2.0~2.5kg of weight；Animal male and female dual-purpose requires selection Animal Sex according to different tests.Animal origin is in Guangxi
Medical university.Rearing conditions: 25 ± 5 DEG C of room temperature, humidity 60 ± 20%.Feed is provided, freely ingests, drink water.
2. experimental method
2.1 analgesic test
2.1.1 hot plate method will first be vented beaker in water bath, and amount of water is not floated in water bath with beaker falls partially to spend, if
Determining water bath temperature is 55 DEG C, and after temperature rises to 55 DEG C and stablizes, taking 100 weight is that 19~21g female mice is placed in sky
In beaker, the incubation period (second) for licking metapedes reaction with mouse, for pain indicator, it is small between 5 seconds and 30 seconds to select the response latency
Mouse (mouse of jump is rejected) measures and records the pain sensation response latency of every mouse (as pain threshold before medicine).It will
Qualified mice is randomly divided into 3 high dose group of blank control group, positive drug control group (acetylsalicylic acid tablet) and embodiment, low dose
Amount group, comparative example high dose group, every group 12.Start stomach-filling after grouping and give corresponding test medicine, 1 time a day, even
It is 3 days continuous, the pain sensation response latency (person was in terms of 60 seconds in greater than 60 seconds) of each group mouse is measured after 1 hour, after the last administration with medicine
Medicine analgesic effect is evaluated with the pain threshold difference before medicine afterwards (if being negative value with the pain threshold difference before medicine after medicine in terms of 0).
2.2.2 writhing method takes mouse 60,19~21g of weight, be randomly divided into blank control group, positive drug control group (Ah
Take charge of a forest tract) and 3 high dose group of embodiment, low dose group, comparative example group high dose group, every group 12.It is filled after grouping
Stomach gives corresponding test medicine, and 1 time a day, for three days on end, every mouse difference 0.6% acetic acid of ip is molten after last dose 1 hour
Liquid 0.5ml, there is writhing response number in each every group of every mouse in 15 minutes from after injection acetum for observation, evaluates drug town
Pain effect.
2.2 anti-inflammatory effect
2.2.1 the influence of Carrageenan rat podarthrum swelling
50 rats are taken, it is high to be randomly divided into blank control group, positive drug control group (acetylsalicylic acid tablet) and embodiment 3
Dosage group, low dose group, comparative example high dose group, every group 10.After animal packet, by method with rat start stomach-filling to
Corresponding test drug is given, 1 time a day, for three days on end.Administration in 3rd day is thick with every rat right hind leg vola pedis of vernier caliper measurement simultaneously
It spends (mm), as control value before medicine.1% carrageenan is subcutaneously injected in 30 minutes every group of every rat right hind leg vola pedis after administration
0.1ml/ only causes inflammation, cause it is scorching after 2,4,6h measure every rat right hind leg vola pedis thickness (mm) again so that right hind foot after inflammation
Right hind vola pedis calculates swelling rate with a thickness of swelling before plantar thickness-cause is scorching.
3. the influence pair rabbit kneecap joint
2.0~2.5kg of weight new zealand rabbit 30 is selected, half male and half female is randomly divided into Normal group, model by gender
Control group, positive controls (acetylsalicylic acid tablet), 3 high dose group of embodiment, low dose group, comparative example high dose group, often
Group 6.It is in addition to Normal group, remaining 1.6% papain physiology salt of each group rabbit right Injection in knuckle articular cavity is water-soluble
Liquid 0.5ml was injected once, co-injection 2 times again every 3 days.Modeling simultaneously, rabbit gives relative medicine by a group stomach-filling, daily 1
It is secondary, continuous 4 weeks.The 2nd day after the last administration, rabbit is put to death, kneecap joint tissue is taken, first substantially visually observes cartilage, then put
Enter and impregnated in the formalin solution that volume fraction is 10%, row Hematoxylin-eosin (HE) dyes, and light is related under the microscope
Morphological change, and carry out histological scores.
3 experimental results
3.1 analgesic activity
Hot plate method test result shows that 3 high dose group of embodiment has apparent analgesic activity, compared with positive controls, right
The mouse hot-plate reaction time can be extended than embodiment high dose group, it is statistically significant compared with blank control group, it is shown in Table 2.
Writhing method test result shows that 3 high dose group of embodiment has apparent analgesic activity, can significantly reduce mouse acetic acid injection and draw
The writhing number risen, it is statistically significant compared with blank control group, it is shown in Table 3.
The analgesic activity result table of 2 hot plate method of table
Compared with blank control group: P < 0.01 * P < 0.05, * *
The analgesic activity result table of 3 writhing method of table
Compared with blank control group: P < 0.01 * P < 0.05, * *
3.2 anti-inflammatory effect
Test result shows that 3 high dose group of the embodiment of the present invention, low dose group and comparative example high dose group are diagonally pitched
Dish glue causes the swelling of rat toes to have obvious anti-inflammatory effect, is shown in Table 4.
The influence result table of 4 Carrageenan of table cause rat toes swelling
Compared with blank control group: P < 0.01 * P < 0.05, * *
3.2.1 to the influence in rabbit kneecap joint
The embodiment of the present invention 3,5, which can reduce rabbit knee Osteoarthritis, to be shown to rabbit kneecap joint test result
Pathological change, can reduce Papain enzymic rabbit knee osteoarthritis model articular cartilage pathology scoring score value, see
Table 5.
The influence result table that table 5 scores to rabbit articular cartilage pathology
Compared with Normal group:▲▲P<0.01；Compared with model control group: P < 0.01 * P < 0.05, * *
Five, clinical application is tested
1. case selection
Selection is because of the functions of joint such as sciatica, affected nerves of patients with trigeminal neuralgia or rheumatism, arthritis, urarthritis
Impaired patients.
2. therapeutic scheme
(1) scheme one:
Affected part is dried with warm water cleaning, 1 CAULIS SCHEFFLERAE KWANGSIENSIS tincture of embodiment is stained with sterilized cotton ball or cotton swab and embrocates affected part simultaneously
It is gently massaged into fever, 3 times a day, drug application can be soaked with double gauze compared with severe one and paste affected part, with bandaging, and 3 times a day will
Medicine drop allows it to penetrate into outside wrapping cloth.
(2) scheme two:
Affected part is dried with warm water cleaning, 3 CAULIS SCHEFFLERAE KWANGSIENSIS tincture of embodiment is stained with sterilized cotton ball or cotton swab and embrocates affected part simultaneously
It is gently massaged into fever, 3 times a day, drug application can be soaked with double gauze compared with severe one and paste affected part, with bandaging, and 3 times a day will
Medicine drop allows it to penetrate into outside wrapping cloth.
3. clinical efficacy
82 patients for meeting above-mentioned case selection condition are selected, male: 40, female: 42, the age: 15-65 years old.Respectively
It is treated using above-mentioned 2 kinds of therapeutic schemes, is as a result detailed in following table.
Clinical efficacy investigation result table
Clinical test results show: using the above-mentioned case of Chinese medicine preparation treatment of present invention treatment pain, cure rate 50%
More than, 97.4% or more effective percentage is significant in efficacy, is showed no irritative response to normal and damaged skin, also there are no skin
Skin allergic reaction, use are safe.
4. typical case
(1) it is blue certain, female 36 years old, because getting injured by a fall the right carpal joint swelling and pain of cause, limitation of activity, is diagnosed as right distal radius closed
Fracture is gone home after local commune hospital doctor manipulative reduction is fixed, and more than 10 days of (specifically unknown) external application of herbal medicine of using by oneself do not improve
And examine, right type joint swelling, tenderness, Pi Wen is slightly higher, each finger joint limitation of activity.Using two external application for curing of scheme, after 20 days, swell
Swollen, pain disappears, and articulations digitorum manus is normal without tenderness, activity, recovery from illness.
(2) poplar, male, 55 years old, two years ago caused left lumbar pain, limitation of activity because of lift weight.It is controlled using being wiped outside scheme one
It treats, pain relief after 5 days, pain disappears after 15 days, and waist activity is normal, recovery from illness.
(3) Tang, male, 58 years old, sciatica was distributed outside and foot after back of thighs, shank.Using present invention side
Treatment is wiped outside case two.Pain relief after 5 days is fully recovered after 15 days, can normal walking and movement.
The above content is combine it is specific/further detailed description of the invention for preferred embodiment, cannot
Assert that specific implementation of the invention is only limited to these instructions.General technical staff of the technical field of the invention is come
It says, without departing from the inventive concept of the premise, some replacements or modifications can also be made to the embodiment that these have been described,
And these substitutions or variant all shall be regarded as belonging to protection scope of the present invention.
Claims (10)
1. a kind of Chinese materia medica preparation for treating pain, which is characterized in that the preparation method of the traditional Chinese medicine preparation comprises the following steps of: CAULIS SCHEFFLERAE KWANGSIENSIS medicinal material, warp
After crushing, is infiltrated, then use 30-40% ethyl alcohol heating and refluxing extraction, filtered, filtrate is cold at 0-4 DEG C with 5% sodium bicarbonate solution
24-48 hours are stood in library, is filtered, medicinal extract is obtained after decompression filtrate recycling ethanol, adds water, is mixed, being adjusted with acid solution ph is
3-4, then the butanol solution extraction being saturated with water, extract liquor is recovered under reduced pressure n-butanol, obtains medicinal extract, adds 50% ethyl alcohol, sweet
Oil, osmanthus nitrogenKetone, mix, filtration to get.
2. the Chinese materia medica preparation for the treatment of pain according to claim 1, which is characterized in that by the supplementary material of following parts by weight
It is made: 3000 parts of CAULIS SCHEFFLERAE KWANGSIENSIS medicinal material, 50% ethyl alcohol 1000ml, glycerol 30ml, osmanthus nitrogenKetone 20ml.
3. a kind of preparation method of the Chinese materia medica preparation for the treatment of pain as described in claim 1, which is characterized in that including following step
It is rapid:
S1. 3000 parts of CAULIS SCHEFFLERAE KWANGSIENSIS medicinal material are taken, is crushed, is infiltrated with 5% sodium bicarbonate solution, then heated back with 30-40% ethyl alcohol
Stream extracts, and filtration obtains primary filtrate；
S2. primary filtrate is stood 24-48 hours in 0-4 DEG C of freezer, filtered, medicinal extract is obtained after decompression filtrate recycling ethanol；
S3. water is added in medicinal extract, mixes, being adjusted with acid solution ph is 3-4；
S4. the butanol solution extraction being saturated with water, extract liquor are recovered under reduced pressure n-butanol, obtain CAULIS SCHEFFLERAE KWANGSIENSIS active component；
S5. 50% ethyl alcohol 1000ml, glycerol 30ml, osmanthus nitrogen are added in CAULIS SCHEFFLERAE KWANGSIENSIS active componentKetone 20ml is mixed, filtration, i.e.,
?.
4. the preparation method of the Chinese materia medica preparation for the treatment of pain according to claim 3, which is characterized in that in the step S1
CAULIS SCHEFFLERAE KWANGSIENSIS medicinal material is crushed into coarse powder.
5. the preparation method of the Chinese materia medica preparation for the treatment of pain according to claim 3, which is characterized in that in the step S1
The dosage of 5% sodium bicarbonate solution is that 1-1.2 times of medicinal material weight is measured, and infiltrating time is 20-30 minutes.
6. the preparation method of the Chinese materia medica preparation for the treatment of pain according to claim 3, which is characterized in that in the step S1
Heating and refluxing extraction number is 2-3 times.
7. the preparation method of the Chinese materia medica preparation for the treatment of pain according to claim 3, which is characterized in that in the step S1
6-8 times that 30-40% amount of alcohol is CAULIS SCHEFFLERAE KWANGSIENSIS medicinal material weight is added to measure.
8. the preparation method of the Chinese materia medica preparation for the treatment of pain according to claim 3, which is characterized in that in the step S3
Amount of water is that 4-6 times of medicinal extract weight is measured, and the acid is hydrochloric acid or sulfuric acid.
9. the preparation method of the Chinese materia medica preparation for the treatment of pain according to claim 3, which is characterized in that in the step S4
The butanol solution being saturated with water extracts 2-3 times.
10. the application of the Chinese materia medica preparation for the treatment of pain as described in claim 1, it is characterised in that: in the treatment pain
Medicine preparation is in the application for preventing or treating pain symptom.
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Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106727796A (en) * | 2015-11-19 | 2017-05-31 | 哈尔滨圣吉药业股份有限公司 | A kind of CAULIS SCHEFFLERAE KWANGSIENSIS dispersible tablet and preparation method thereof |
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2018
- 2018-09-30 CN CN201811163009.9A patent/CN109010397A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106727796A (en) * | 2015-11-19 | 2017-05-31 | 哈尔滨圣吉药业股份有限公司 | A kind of CAULIS SCHEFFLERAE KWANGSIENSIS dispersible tablet and preparation method thereof |
Non-Patent Citations (1)
| Title |
|---|
| 刘娴，等: "汉桃叶抗炎镇痛作用有效部位的筛选", 《安徽医药》 * |
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Application publication date: 20181218 |