CN109007811A - A kind of composition and preparation method thereof of probiotics fish oil - Google Patents
A kind of composition and preparation method thereof of probiotics fish oil Download PDFInfo
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- CN109007811A CN109007811A CN201810943778.4A CN201810943778A CN109007811A CN 109007811 A CN109007811 A CN 109007811A CN 201810943778 A CN201810943778 A CN 201810943778A CN 109007811 A CN109007811 A CN 109007811A
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- powder
- fish oil
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- freeze
- probiotics
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Classifications
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- A—HUMAN NECESSITIES
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- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/115—Fatty acids or derivatives thereof; Fats or oils
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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Abstract
The invention discloses a kind of compositions and preparation method thereof of probiotics fish oil, main component includes: in parts by mass, product can be obtained in afore-mentioned materials by 100 parts of Freeze-dry Powder of Probioctics composition, 1-100 parts of prebiotic bacterium exopolysaccharide freeze-dried powder composition, 1-100 parts of fish oil composition, 1-10 parts of xylo-oligosaccharide, 1-10 parts of oligofructose, 1-10 parts of inulin, 1-10 parts of soluble dietary fiber, 1-10 parts of insoluble diedairy fiber after mixing.The optimum composition method of fish oil and probiotics is obtained by inquiring into preparation method, improves the content of active bacteria.
Description
Technical field
The present invention relates to the compositions of a kind of probiotics and fish oil.
Background technique
Hyperlipidemia refers to the case where lipoprotein abnormalities elevated levels in blood, with the improvement of people's living standards,
The diseases such as the following hyperlipidemia, artery sclerosis increasingly influence and endanger people's lives and health.Obesity is high blood
The most direct external performance of rouge, 90% or more obese patient have the complication such as hyperlipidemia
After fat pathogenesis is with enteric microorganism imbalance, level of endotoxin excessively increases directly related.Typical item
Part pathogenic bacteria include enterobacter cloacae, pseudomonas aeruginosa and sulfate reducing bacteria of enterobacteriaceae etc..When immune system goes out
Existing problem, these conditioned pathogens will lead to a series of symptoms such as enteron aisle.Enteron aisle chronic disease is adjusted increasingly with probiotics
It attracts attention
But there are no the compositions for a kind of probiotics fish oil occur currently on the market, dedicated for treating or preventing
Or conditioning hyperlipidemia.
Summary of the invention
The purpose of the present invention is to provide one kind can prevent and control the probiotics of hyperlipidemia and the composition of fish oil, by force
Change intestinal flora and adjust function, improve immunity of organisms, induced by endotoxin is horizontal, and partial condition pathogenic bacteria, which have, to be adjusted and inhibit to make
With the application effectively combines fish oil and probiotics, the effect of combination is obtained on taking effect, while to the system of product
Preparation Method improves, to prepare various forms of products.
The present invention solves the above problems used technical solution are as follows:
A kind of composition of probiotics fish oil, primary raw material include: Freeze-dry Powder of Probioctics composition 100 in parts by mass
Part, 1-100 parts of prebiotic bacterium exopolysaccharide freeze-dried powder composition, 1-100 parts of fish oil composition, 1-10 parts of xylo-oligosaccharide, oligomeric fruit
1-10 parts sugared, 1-10 parts of inulin, 1-10 parts of soluble dietary fiber, 1-10 parts of insoluble diedairy fiber, afore-mentioned materials are mixed
Product can be obtained after uniformly.
Specifically, the preparation method of the Freeze-dry Powder of Probioctics composition is will to be selected from lactobacillus plantarum, rhamnose cream bar
Bacterium, Lactobacillus casei, lactobacillus reuteri, lactobacillus acidophilus, bifidobacterium lactis, bifidobacterium longum, bifidobacterium breve, not tally pair
One of discrimination bacillus or a variety of fermented cultures, centrifugation and freeze-drying obtain the Freeze-dry Powder of Probioctics composition of high activity.
Preferably, strain is linked into 1-50% containing balsam pear powder, lectuce tea powder 1-50%, Huang with the inoculum concentration of 1-50wt%
Melon powder 1-50%, yam flour 1-50%, coptis powder 1-50%, radix scutellariae powder 1-50%, astragalus membranaceus powder 1-50%, rhizoma polygonati powder 1-50%,
Fructus Schisandrae Chinensis powder 1-50%, Rhizoma Atractylodis Macrocephalae powder 1-50%, hawthorn powder 1-50%, oligofructose 1-10%, oligoisomaltose 1-10%, chrysanthemum
Powder 1-10%, chitosan oligosaccharide 1-10%, resistant dextrin 1-10%, xylitol 1-10%, lactitol 1-10%, antierythrite 1-
10%, it in one of D-sorbite 1-10% or the culture of numerous compositions, after 25-45 DEG C of fermentation 12-240h, obtains
Fermentation liquid is centrifuged by fermentation liquid, adds inulin 10-80%, resistant dextrin 10-80%, polydextrose 10- after removing supernatant
80%, it is freeze-dried 48-96h after mixing, is prepared into Freeze-dry Powder of Probioctics composition.
Specifically, the preparation method of the prebiotic bacterium exopolysaccharide freeze-dried powder composition is with Lactobacillus casei, long bifid
In the medium through 25-40 DEG C of fermentation 12-48h, culture medium generates exocellular polysaccharide for bacillus and streptococcus thermophilus, through alcohol precipitation,
Freeze-drying obtains exocellular polysaccharide freeze-drying compound powder after centrifugation removal supernatant；The mass percent of the culture medium matches:
Mannose 1-10%, xylose 1-10%, melezitose 1-10%, gossypose 1-10%, xylitol 1-10%, lactitol 1-10%,
Antierythrite 1-10%, PURE WHEY 1-10%, soybean protein isolate 1-10%, Evamilk albumen 1-10%, milk point
From albumen 1-10%, whole-fat milk powder 1-10%, skimmed milk powder 1-10%, pea protein 1-10%, rice protein 1-10%, blueberry
One of juice 1-10%, Tomato juice 1-10%, strawberry juice 1-10%, brewer's wort 1-10%, mulberries powder 1-10% are more
Kind.
Specifically, the fish oil composition is fish oil, fish oil refined oil, the spray-dried obtained mixture of krill oil.
Preferably, the fish oil composition is mixed in the preparation process of Freeze-dry Powder of Probioctics composition, specific to grasp
As lactobacillus plantarum, Lactobacillus rhamnosus, Lactobacillus casei, lactobacillus reuteri, lactobacillus acidophilus, newborn bifid bar will be selected from
One of bacterium, bifidobacterium longum, bifidobacterium breve, bifidobacterium bifidum or a variety of fermented cultures, centrifugation and freeze-drying obtain
The Freeze-dry Powder of Probioctics composition of high activity；Strain is specifically linked into 1- containing balsam pear powder with the inoculum concentration of 1-50wt%
50%, lectuce tea powder 1-50%, cucumber powder 1-50%, yam flour 1-50%, coptis powder 1-50%, radix scutellariae powder 1-50%, Radix Astragali
Powder 1-50%, rhizoma polygonati powder 1-50%, Fructus Schisandrae Chinensis powder 1-50%, Rhizoma Atractylodis Macrocephalae powder 1-50%, hawthorn powder 1-50%, oligofructose 1-
10%, oligoisomaltose 1-10%, inulin 1-10%, chitosan oligosaccharide 1-10%, resistant dextrin 1-10%, xylitol 1-10%,
In one of lactitol 1-10%, antierythrite 1-10%, D-sorbite 1-10% or the culture of numerous compositions, warp
After 25-45 DEG C of fermentation 12-240h, fermentation liquid is obtained, fermentation liquid is centrifuged, adds fish oil, fish oil refining after removing supernatant
Oil, the spray-dried obtained mixture of krill oil, are freeze-dried 48-96h after mixing, are prepared into the probiotics containing fish oil
Freeze-dried powder composition film.
In order to convenient to take and convenient for saving or carrying, best bet is that product is prepared to powdering, but fish oil powder
It is easy to happen reunion and agglomerates, therefore to improve the dispersibility of fish oil powder in the product as far as possible in process of production,
Fish oil, fish oil refined oil, krill oil are added in tunning by the application, and then freeze-dried powder, probiotics is made in freeze-drying together
It is sufficiently mixed in freeze-drying process with fish oil etc., fish oil sweeps along probiotics, and such particle is less likely to occur to reunite, dispersibility
It is good.In addition, emulsifier, protectant use can be saved using the above method, directly protected using fish oil as emulsifier and thallus
Agent is protected, thus the activity of the probiotics helped to improve.
Fish oil composition is allowed to mix in the preparation process of prebiotic bacterium exopolysaccharide freeze-dried powder composition furthermore it is also possible to design
Preparation is closed, to achieve the purpose that prevent fish oil powder from agglomerating.Concrete operations are, by Lactobacillus casei, bifidobacterium longum and thermophilic
For streptococcus in the medium through 25-40 DEG C of fermentation 12-48h, culture medium generates exocellular polysaccharide, through in alcohol precipitation, centrifugation removal
After clear liquid, fish oil, fish oil refined oil, krill oil is added, the exocellular polysaccharide that then freeze-drying obtains being mixed with fish oil together freezes
Dry powder composite；The mass percent of the culture medium matches: mannose 1-10%, xylose 1-10%, melezitose 1-10%, cotton
Sub- sugar 1-10%, xylitol 1-10%, lactitol 1-10%, antierythrite 1-10%, PURE WHEY 1-10%, soybean separation
Albumen 1-10%, Evamilk albumen 1-10%, milk protein isolate 1-10%, whole-fat milk powder 1-10%, skimmed milk powder 1-
10%, pea protein 1-10%, rice protein 1-10%, blueberry juice 1-10%, Tomato juice 1-10%, strawberry juice 1-10%,
One or more of brewer's wort 1-10%, mulberries powder 1-10%.
Preferably, present inventor also tests out Freeze-dry Powder of Probioctics composition and the fish oil composition side of being mixed with
Method: prepare the aqueous emulsion of 1-10% containing fish oil, fish oil refined oil 1-10%, krill oil 1-10%, combine Freeze-dry Powder of Probioctics
Object, which is added in the aqueous emulsion, forms emulsion, and emulsion is then spray-dried powder processed；The preparation of the Freeze-dry Powder of Probioctics
Being will be selected from lactobacillus plantarum, Lactobacillus rhamnosus, Lactobacillus casei, lactobacillus reuteri, lactobacillus acidophilus, newborn bifid bar
One of bacterium, bifidobacterium longum, bifidobacterium breve, bifidobacterium bifidum or a variety of fermented cultures, centrifugation and freeze-drying obtain
The Freeze-dry Powder of Probioctics composition of high activity.Freeze-dry Powder of Probioctics and fish oil etc. are prepared into emulsion, then spray drying allows
Particle of fish oil is adsorbed on probiotics, is formed and is coated to probiotics, improves the activity of probiotics.
The present invention provides the compositions of a kind of probiotics and fish oil powder by the application, can be changed by taking the combination for a long time
Become the composition of enteric microorganism, adjust intestinal flora, improves intestinal immunity, taken suitable for hyperlipemic patients, and pass through
Relevant immune factor and level of endotoxin are measured, it can qualitative and the quantitative reaction vivo immunization factor variation.
The application further improves the preparation method of probiotics and fish oil composition, improves depositing for probiotics
Motility rate, while solving the problems, such as the agglomeration of fish oil powder.
Detailed description of the invention
Fig. 1 is the experimental result picture that embodiment 6 detects target TG；
Fig. 2 is the experimental result picture that embodiment 6 detects target TC.
Specific embodiment
Present invention is further described in detail with reference to embodiments.
This application involves a kind of composition of probiotics fish oil, ingredient includes: Freeze-dry Powder of Probioctics combination in parts by mass
100 parts of object, prebiotic 100 parts of bacterium exopolysaccharide freeze-dried powder composition, 100 parts of fish oil composition, 10 parts of xylo-oligosaccharide, oligofructose
10 parts, 10 parts of inulin, 10 parts of soluble dietary fiber, 10 parts of insoluble diedairy fiber.Freeze-dry Powder of Probioctics composition, probiotics
Compound powder, fish oil composition is lyophilized as main component, xylo-oligosaccharide, oligofructose, inulin, soluble dietary in exocellular polysaccharide
Fiber, insoluble diedairy fiber are as submember.Ratio between main component is 100:(1~100): (1~100).It is secondary
Wanting the ratio between ingredient is (1~10): (1~10): (1~10).Main component and the ratio of submember be 100:(1~
5)。
Embodiment 1
The preparation method of probiotics fish oil composition, includes the following steps
(1) Freeze-dry Powder of Probioctics composition: by lactobacillus plantarum, Lactobacillus rhamnosus, Lactobacillus casei, not tally bifid bar
Bacterium is linked into 1-50% containing balsam pear powder, lectuce tea powder 1-50%, cucumber powder 1-50%, yam flour 1- with the inoculum concentration of 1-50wt%
50%, coptis powder 1-50%, radix scutellariae powder 1-50%, astragalus membranaceus powder 1-50%, rhizoma polygonati powder 1-50%, Fructus Schisandrae Chinensis powder 1-50%, Rhizoma Atractylodis Macrocephalae
Powder 1-50%, hawthorn powder 1-50%, oligofructose 1-10%, oligoisomaltose 1-10%, inulin 1-10%, chitosan oligosaccharide 1-
10%, resistant dextrin 1-10%, xylitol 1-10%, lactitol 1-10%, antierythrite 1-10%, in D-sorbite 1-10%
One or more compositions culture in, after 25-45 DEG C of fermentation 72h, obtain fermentation liquid, fermentation liquid is centrifuged, remove
Supernatant is added inulin 10%, resistant dextrin 20%, polydextrose 10%, is freeze-dried 48-96h after mixing, is prepared into
Freeze-dry Powder of Probioctics composition.
(2) it prebiotic bacterium exopolysaccharide freeze-dried powder composition: is being trained with Lactobacillus casei, bifidobacterium longum and streptococcus thermophilus
It supports through 25-40 DEG C of fermentation 12-48h in base, culture medium generates exocellular polysaccharide, freezes after removing supernatant with alcohol precipitation, centrifugation
It is dried to obtain exocellular polysaccharide freeze-drying compound powder.The mass percent of culture medium matches: mannose 1-10%, xylose 1-10%,
Melezitose 1-10%, gossypose 1-10%, xylitol 1-10%, lactitol 1-10%, antierythrite 1-10%, PURE WHEY
1-10%, soybean protein isolate 1-10%, Evamilk albumen 1-10%, milk protein isolate 1-10%, whole-fat milk powder 1-
10%, skimmed milk powder 1-10%, pea protein 1-10%, rice protein 1-10%, blueberry juice 1-10%, Tomato juice 1-
10%, one or more of strawberry juice 1-10%, brewer's wort 1-10%, mulberries powder 1-10%.
(3) fish oil composition: mass percent is matched: fish oil 10%, fish oil refined oil 10%, krill oil 10%, warp
Spray drying obtains fish oil compound powder.
(4) by step 1-3 resulting product and xylo-oligosaccharide, oligofructose, inulin, soluble dietary fiber, insoluble
Dietary fiber mixes in proportion to obtain the final product.
Embodiment 2
The preparation method of probiotics fish oil composition, includes the following steps
(1) fish oil composition and Freeze-dry Powder of Probioctics composition are mixed with: will selected from lactobacillus plantarum, rhamnose cream
Bacillus, bifidobacterium longum, bifidobacterium breve, is linked into bifidobacterium bifidum bifidobacterium lactis with the inoculum concentration of 1-50wt%
1-50% containing balsam pear powder, lectuce tea powder 1-50%, cucumber powder 1-50%, yam flour 1-50%, coptis powder 1-50%, radix scutellariae powder 1-
50%, astragalus membranaceus powder 1-50%, rhizoma polygonati powder 1-50%, Fructus Schisandrae Chinensis powder 1-50%, Rhizoma Atractylodis Macrocephalae powder 1-50%, hawthorn powder 1-50%, oligomeric
Fructose 1-10%, oligoisomaltose 1-10%, inulin 1-10%, chitosan oligosaccharide 1-10%, resistant dextrin 1-10%, xylitol 1-
10%, one of lactitol 1-10%, antierythrite 1-10%, D-sorbite 1-10% or the culture of numerous compositions
In, after 25-45 DEG C of fermentation 12-96h, fermentation liquid is obtained, fermentation liquid is centrifuged, adds fish oil, fish oil after removing supernatant
Refined oil, krill oil, emulsification are stood, and then spray-dried obtained mixture, is freeze-dried 48-96h after mixing, system
For at the Freeze-dry Powder of Probioctics composition containing fish oil, in spray-drying process, fish oil is coated on probiotics surface.
(2) prebiotic bacterium exopolysaccharide freeze-dried powder composition: referring to embodiment 1.
(3) by step 1 and 2 products obtained therefroms and xylo-oligosaccharide, oligofructose, inulin, soluble dietary fiber, insoluble meals
Food fiber mixes in proportion to obtain the final product.
Embodiment 3
The preparation method of probiotics fish oil composition, includes the following steps
(1) Freeze-dry Powder of Probioctics composition: referring to embodiment 1.
(2) the combination preparation of fish oil composition, prebiotic bacterium exopolysaccharide freeze-dried powder, by Lactobacillus casei, bifidobacterium longum
With streptococcus thermophilus in the medium through 25-40 DEG C of fermentation 12-48h, culture medium generates exocellular polysaccharide, through alcohol precipitation, centrifugation
After removing supernatant, fish oil, fish oil refined oil, krill oil is added, then freeze-drying obtains being mixed with the extracellular of fish oil together
Polysaccharide frozen dried powder composition；The mass percent of culture medium matches: mannose 1-10%, xylose 1-10%, melezitose 1-10%,
Gossypose 1-10%, xylitol 1-10%, lactitol 1-10%, antierythrite 1-10%, PURE WHEY 1-10%, soybean point
From albumen 1-10%, Evamilk albumen 1-10%, milk protein isolate 1-10%, whole-fat milk powder 1-10%, skimmed milk powder 1-
10%, pea protein 1-10%, rice protein 1-10%, blueberry juice 1-10%, Tomato juice 1-10%, strawberry juice 1-10%,
One or more of brewer's wort 1-10%, mulberries powder 1-10%.
(3) by step 1 and 2 products obtained therefroms and xylo-oligosaccharide, oligofructose, inulin, soluble dietary fiber, insoluble meals
Food fiber mixes in proportion to obtain the final product.
Embodiment 4
The preparation method of probiotics fish oil composition, includes the following steps
(1) Freeze-dry Powder of Probioctics composition and fish oil composition mixing preparation method: prepare 1-10% containing fish oil, ichthyol
The aqueous emulsion for refining oil 1-10%, krill oil 1-10%, Freeze-dry Powder of Probioctics composition is added in the aqueous emulsion and forms milkiness
Then emulsion is spray-dried powder processed by liquid；The preparation of the Freeze-dry Powder of Probioctics is will be selected from lactobacillus plantarum, rhamnose cream
It is bacillus, Lactobacillus casei, lactobacillus reuteri, lactobacillus acidophilus, bifidobacterium lactis, bifidobacterium longum, bifidobacterium breve, not tally
One of Bifidobacterium or a variety of fermented cultures, centrifugation and freeze-drying obtain the Freeze-dry Powder of Probioctics composition of high activity；Training
It supports with embodiment 1
(2) prebiotic bacterium exopolysaccharide freeze-dried powder composition: referring to embodiment 1.
(3) by step 1 and 2 products obtained therefroms and xylo-oligosaccharide, oligofructose, inulin, soluble dietary fiber, insoluble meals
Food fiber mixes in proportion to obtain the final product.
The viable bacteria concentration (CFU/g) of embodiment 1-4
The viable count of different preparation methods, product is also different, and it is prebiotic to prepare product using embodiment 4 and embodiment 2
The survival rate of bacterium is higher.
Embodiment 5
Choose 50 people of hypertensive patient, all cholesterol and triglycerides preference crowd, Diagnostic Value of Fasting Serum total cholesterol
(TC) > 6.5mml/L, triglycerides (TG) > 1.24mml/L.Compound Evaluation of Diagnostic Criteria of Hypertension: diastolic pressure >=90mmHg.It is clinical
Show as headache, dizziness.Age 40-55 years old, average age 50.2 years old, wherein male 30, women 20.Control group and experiment
Group men and women patient is fifty-fifty, and two groups of average ages are suitable.
Treatment method: experimental group continuously takes probiotics fish oil product 28 days of embodiment 4, and daily 30 grams, control group takes
With common fish oil.
Observation index: effective: clinical symptoms disappear, diastole drops >=10mmHg, and TC declines > 20%, and TG declines >
40%；
Effective: clinical symptoms are obviously improved, diastole drops >=10mmHg, and TC declines > 10~20%, and TG declines > 20
~40%；
Invalid: clinical symptoms are not improved, and TC declines < 10%, and TG is dropped by less than 20%.
Test result:
1. Comparison of therapeutic: the total effective rate of experimental group and control group is respectively 92% and 64%, the total effective rate of experimental group
The obvious effective rate for being apparently higher than control group (p < 0.05) and experimental group is equally apparently higher than control group (p < 0.05).
The Comparison of therapeutic of experimental group and control group
| Group | Number of cases | Significantly | Effectively | In vain | Total effective rate |
| Control group | 25 | 13 | 10 | 2 | 92% |
| Experimental group | 25 | 10 | 6 | 7 | 64 |
Blood lipids index comparison: experimental group and control group test preceding TG and TC value and compare equal no difference of science of statistics (p > 0.05),
TG and TC value is substantially reduced (p < 0.05) after test, and comparison has statistical difference (p < 0.05) between group in group.Simultaneously
Experimental group diastolic pressure drop-out value is significantly greater than control group.
Experimental group and the blood lipids index of control group change table
Embodiment 6
With above-mentioned probiotics fish oil stomach-filling, taking dose be daily 3ml/ only, every group 10, totally 4 groups, continuous gavage 56
It；5 week old male rats 40, freely feed to the 56th day, are equally divided into 4 groups, every group 10:
Probiotics fish oil group: stomach-filling viable bacteria suspension feeds high lipid food；Normal group: 0.85% physiological saline of stomach-filling, feeding
Standard feed；Control group: 0.85% physiological saline of stomach-filling feeds high lipid food
Daily every 2ml stomach-filling, continues 56 days, then stops stomach-filling, each group feed, water are constant to continue 56 days.
3, sample acquisition is tested with analysis:
Before formal test, the 56th day and period, blood was collected.Blood-sampling method is after going on a hunger strike a night, in rat femoral vein
Blood sampling, 4000r/m is centrifuged 10min and separates serum after blood coagulation, using kit and chromotropic acid development process, respectively cholesterol detection and
Triglycerides.
4, experimental result
See Fig. 1,2.Femoral vein blood drawing detection after being fed mouse high in fat 56 days with the suspension of fish oil containing probiotics, finds and compares
Decline 23.2% and 28.5% respectively compared to triglycerides in mouse blood and cholesterol, shows probiotics fish oil in Mice Body
Truly having reduces cholesterol and triglycerides effect.
Claims (8)
1. a kind of composition of probiotics fish oil, it is characterised in that: main component includes: Freeze-dry Powder of Probioctics in parts by mass
100 parts of composition, 1-100 parts of prebiotic bacterium exopolysaccharide freeze-dried powder composition, 1-100 parts of fish oil composition, xylo-oligosaccharide 1-10
Part, 1-10 parts of oligofructose, 1-10 parts of inulin, 1-10 parts of soluble dietary fiber, 1-10 parts of insoluble diedairy fiber, will be aforementioned
Product can be obtained in material after mixing.
2. the composition of probiotics fish oil according to claim 1, it is characterised in that: the Freeze-dry Powder of Probioctics composition
Preparation method be that will be selected from lactobacillus plantarum, Lactobacillus rhamnosus, Lactobacillus casei, lactobacillus reuteri, acidophilus cream bar
One of bacterium, bifidobacterium lactis, bifidobacterium longum, bifidobacterium breve, bifidobacterium bifidum or a variety of fermented cultures, centrifugation
The Freeze-dry Powder of Probioctics composition of high activity is obtained with freeze-drying.
3. the composition of probiotics fish oil according to claim 2, it is characterised in that: by strain with the inoculation of 1-50wt%
Amount is linked into 1-50% containing balsam pear powder, lectuce tea powder 1-50%, cucumber powder 1-50%, yam flour 1-50%, coptis powder 1-50%,
Radix scutellariae powder 1-50%, astragalus membranaceus powder 1-50%, rhizoma polygonati powder 1-50%, Fructus Schisandrae Chinensis powder 1-50%, Rhizoma Atractylodis Macrocephalae powder 1-50%, hawthorn powder 1-
50%, oligofructose 1-10%, oligoisomaltose 1-10%, inulin 1-10%, chitosan oligosaccharide 1-10%, resistant dextrin 1-
10%, one of xylitol 1-10%, lactitol 1-10%, antierythrite 1-10%, D-sorbite 1-10% or a variety of groups
In the culture for closing object, after 25-45 DEG C of fermentation 12-240h, fermentation liquid is obtained, fermentation liquid is centrifuged, after removal supernatant again
Inulin 10-80%, resistant dextrin 10-80%, polydextrose 10-80% is added, is freeze-dried 48-96h after mixing, prepares
At Freeze-dry Powder of Probioctics composition.
4. the composition of probiotics fish oil according to claim 1, it is characterised in that: the prebiotic bacterium exopolysaccharide freeze-drying
The preparation method of compound powder is sent out in the medium through 25-40 DEG C with Lactobacillus casei, bifidobacterium longum and streptococcus thermophilus
Ferment 12-48h, culture medium generate exocellular polysaccharide, and freeze-drying obtains exocellular polysaccharide jelly after alcohol precipitation, centrifugation removal supernatant
Dry powder composite；The mass percent of the culture medium matches: mannose 1-10%, xylose 1-10%, melezitose 1-10%, cotton
Sub- sugar 1-10%, xylitol 1-10%, lactitol 1-10%, antierythrite 1-10%, PURE WHEY 1-10%, soybean separation
Albumen 1-10%, Evamilk albumen 1-10%, milk protein isolate 1-10%, whole-fat milk powder 1-10%, skimmed milk powder 1-
10%, pea protein 1-10%, rice protein 1-10%, blueberry juice 1-10%, Tomato juice 1-10%, strawberry juice 1-10%,
One or more of brewer's wort 1-10%, mulberries powder 1-10%.
5. the composition of probiotics fish oil according to claim 1, it is characterised in that: the fish oil composition be fish oil,
The spray-dried obtained mixture of fish oil refined oil, krill oil.
6. probiotics fish oil composition according to claim 1, it is characterised in that: the fish oil composition is frozen in probiotics
It is mixed in the preparation process of dry powder composite, concrete operations are will be selected from lactobacillus plantarum, Lactobacillus rhamnosus, cheese cream
Bacillus, lactobacillus reuteri, lactobacillus acidophilus, bifidobacterium lactis, bifidobacterium longum, bifidobacterium breve, in bifidobacterium bifidum
It is one or more it is fermented culture, centrifugation and freeze-drying obtain the Freeze-dry Powder of Probioctics composition of high activity；Specifically by strain
1-50% containing balsam pear powder, lectuce tea powder 1-50%, cucumber powder 1-50%, yam flour 1- are linked into the inoculum concentration of 1-50wt%
50%, coptis powder 1-50%, radix scutellariae powder 1-50%, astragalus membranaceus powder 1-50%, rhizoma polygonati powder 1-50%, Fructus Schisandrae Chinensis powder 1-50%, Rhizoma Atractylodis Macrocephalae
Powder 1-50%, hawthorn powder 1-50%, oligofructose 1-10%, oligoisomaltose 1-10%, inulin 1-10%, chitosan oligosaccharide 1-
10%, resistant dextrin 1-10%, xylitol 1-10%, lactitol 1-10%, antierythrite 1-10%, in D-sorbite 1-10%
One or more compositions culture in, after 25-45 DEG C of fermentation 12-240h, obtain fermentation liquid, fermentation liquid is centrifuged,
The spray-dried obtained mixture of fish oil, fish oil refined oil, krill oil is added after removal supernatant, is freezed after mixing
Dry 48-96h, is prepared into the Freeze-dry Powder of Probioctics composition containing fish oil.
7. probiotics fish oil composition according to claim 1, it is characterised in that: the fish oil composition is in prebiotic mycetocyte
It is mixed in the preparation process of exo polysaccharides freeze-drying compound powder, concrete operations are by Lactobacillus casei, bifidobacterium longum and thermophilic
For hot streptococcus in the medium through 25-40 DEG C of fermentation 12-48h, culture medium generates exocellular polysaccharide, removes through alcohol precipitation, centrifugation
After supernatant, fish oil, fish oil refined oil, krill oil is added, then freeze-drying obtains the exocellular polysaccharide for being mixed with fish oil together
Compound powder is lyophilized；The mass percent of the culture medium matches: mannose 1-10%, xylose 1-10%, melezitose 1-10%,
Gossypose 1-10%, xylitol 1-10%, lactitol 1-10%, antierythrite 1-10%, PURE WHEY 1-10%, soybean point
From albumen 1-10%, Evamilk albumen 1-10%, milk protein isolate 1-10%, whole-fat milk powder 1-10%, skimmed milk powder 1-
10%, pea protein 1-10%, rice protein 1-10%, blueberry juice 1-10%, Tomato juice 1-10%, strawberry juice 1-10%,
One or more of brewer's wort 1-10%, mulberries powder 1-10%.
8. probiotics fish oil composition according to claim 1, it is characterised in that: Freeze-dry Powder of Probioctics composition and fish oil
Composition mixing preparation method: preparing the aqueous emulsion of 1-10% containing fish oil, fish oil refined oil 1-10%, krill oil 1-10%, will
Freeze-dry Powder of Probioctics composition, which is added in the aqueous emulsion, forms emulsion, and emulsion is then spray-dried powder processed；The benefit
The preparation of raw bacterium freeze-dried powder is will to be selected from lactobacillus plantarum, Lactobacillus rhamnosus, Lactobacillus casei, lactobacillus reuteri, acidophilus
One of lactobacillus, bifidobacterium lactis, bifidobacterium longum, bifidobacterium breve, bifidobacterium bifidum or it is a variety of it is fermented culture,
Centrifugation and freeze-drying obtain the Freeze-dry Powder of Probioctics composition of high activity.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115486540A (en) * | 2021-08-25 | 2022-12-20 | 江中药业股份有限公司 | Probiotic herbal composition with antioxidant effect and preparation method thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101208018A (en) * | 2005-06-27 | 2008-06-25 | 法马罗吉卡公司 | Drink comprising a combination of fish oil and probiotics |
| CN105124333A (en) * | 2015-09-02 | 2015-12-09 | 曹博雅 | Composition containing probiotics as well as preparation method and application of composition |
| CN107158231A (en) * | 2017-06-07 | 2017-09-15 | 马凯 | A kind of composition for the probiotics fermention Chinese herbal medicine for preventing and controlling diabetes |
| CN107365705A (en) * | 2017-07-14 | 2017-11-21 | 江苏微康生物科技有限公司 | A kind of oil-soluble probiotics freezes freeze drying protectant and its application of bacterium powder |
-
2018
- 2018-08-18 CN CN201810943778.4A patent/CN109007811A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101208018A (en) * | 2005-06-27 | 2008-06-25 | 法马罗吉卡公司 | Drink comprising a combination of fish oil and probiotics |
| CN105124333A (en) * | 2015-09-02 | 2015-12-09 | 曹博雅 | Composition containing probiotics as well as preparation method and application of composition |
| CN107158231A (en) * | 2017-06-07 | 2017-09-15 | 马凯 | A kind of composition for the probiotics fermention Chinese herbal medicine for preventing and controlling diabetes |
| CN107365705A (en) * | 2017-07-14 | 2017-11-21 | 江苏微康生物科技有限公司 | A kind of oil-soluble probiotics freezes freeze drying protectant and its application of bacterium powder |
Non-Patent Citations (2)
| Title |
|---|
| KERRY L. BENTLEY-HEWITTAB等: "How fish oils could support our friendly bacteria", 《LIPID TECHNOLOGY》 * |
| 王娜: "巴氏醋杆菌对环境胁迫的生理响应机制及提高冻干存活率的研究", 《中国优秀硕士学位论文全文数据库 工程科技Ⅰ辑》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115486540A (en) * | 2021-08-25 | 2022-12-20 | 江中药业股份有限公司 | Probiotic herbal composition with antioxidant effect and preparation method thereof |
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Application publication date: 20181218 |