CN109007298A - A kind of Radix Ophiopogonis feed for pet additive with U.S. hair effect - Google Patents
A kind of Radix Ophiopogonis feed for pet additive with U.S. hair effect Download PDFInfo
- Publication number
- CN109007298A CN109007298A CN201810736697.7A CN201810736697A CN109007298A CN 109007298 A CN109007298 A CN 109007298A CN 201810736697 A CN201810736697 A CN 201810736697A CN 109007298 A CN109007298 A CN 109007298A
- Authority
- CN
- China
- Prior art keywords
- radix ophiopogonis
- ethyl acetate
- radix
- feed
- supernatant
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000000654 additive Substances 0.000 title claims abstract description 30
- 230000000996 additive effect Effects 0.000 title claims abstract description 29
- 210000004209 hair Anatomy 0.000 title claims abstract description 20
- 230000000694 effects Effects 0.000 title claims abstract description 17
- 241001521474 Liriope <hydrozoan> Species 0.000 claims abstract description 39
- 239000000419 plant extract Substances 0.000 claims abstract description 38
- 230000000975 bioactive effect Effects 0.000 claims abstract description 17
- 239000004615 ingredient Substances 0.000 claims abstract description 17
- 239000002994 raw material Substances 0.000 claims abstract description 8
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 claims description 90
- 239000007788 liquid Substances 0.000 claims description 62
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 49
- 239000006228 supernatant Substances 0.000 claims description 40
- 238000000605 extraction Methods 0.000 claims description 35
- 239000013049 sediment Substances 0.000 claims description 30
- 239000000284 extract Substances 0.000 claims description 27
- 108090000790 Enzymes Proteins 0.000 claims description 25
- 102000004190 Enzymes Human genes 0.000 claims description 25
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 25
- 229940088598 enzyme Drugs 0.000 claims description 25
- 108010059892 Cellulase Proteins 0.000 claims description 24
- 229940106157 cellulase Drugs 0.000 claims description 24
- 239000000843 powder Substances 0.000 claims description 21
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 claims description 20
- 108090000526 Papain Proteins 0.000 claims description 20
- 238000005119 centrifugation Methods 0.000 claims description 20
- 239000012071 phase Substances 0.000 claims description 20
- 239000002244 precipitate Substances 0.000 claims description 20
- 239000008346 aqueous phase Substances 0.000 claims description 19
- 239000002024 ethyl acetate extract Substances 0.000 claims description 19
- 239000004365 Protease Substances 0.000 claims description 17
- 229940055729 papain Drugs 0.000 claims description 17
- 235000019834 papain Nutrition 0.000 claims description 17
- 238000002360 preparation method Methods 0.000 claims description 16
- 229960000935 dehydrated alcohol Drugs 0.000 claims description 13
- 238000001035 drying Methods 0.000 claims description 10
- 238000001914 filtration Methods 0.000 claims description 10
- 238000009777 vacuum freeze-drying Methods 0.000 claims description 10
- 238000005406 washing Methods 0.000 claims description 10
- BXTNNJIQILYHJB-GFCCVEGCSA-N Methylophiopogonanone A Chemical compound C1=C2OCOC2=CC(C[C@@H]2COC3=C(C)C(O)=C(C(=C3C2=O)O)C)=C1 BXTNNJIQILYHJB-GFCCVEGCSA-N 0.000 claims description 8
- FHKHGNFKBPFJCB-UHFFFAOYSA-N (25S)-ruscogenin 1-O-<alpha-L-rhamnopyranosyl(1->2)><beta-D-xylopyranosyl(1->3)>-beta-D-fucopyranoside Natural products O1C2(OCC(C)CC2)C(C)C(C2(CCC3C45C)C)C1CC2C3CC=C4CC(O)CC5OC(C1OC2C(C(O)C(O)C(C)O2)O)OC(C)C(O)C1OC1OCC(O)C(O)C1O FHKHGNFKBPFJCB-UHFFFAOYSA-N 0.000 claims description 7
- FHKHGNFKBPFJCB-LYLKFOBISA-N Ophiopogonin D Chemical compound O([C@H]1[C@@H](O)[C@@H](C)O[C@H]([C@@H]1O[C@H]1[C@@H]([C@H](O)[C@@H](O)[C@H](C)O1)O)O[C@@H]1C[C@H](O)CC2=CC[C@H]3[C@@H]4C[C@H]5[C@@H]([C@]4(CC[C@@H]3[C@]21C)C)[C@@H]([C@]1(OC[C@H](C)CC1)O5)C)[C@@H]1OC[C@@H](O)[C@H](O)[C@H]1O FHKHGNFKBPFJCB-LYLKFOBISA-N 0.000 claims description 7
- IDGCVTONMQMXPU-JHZREZMDSA-N ophiopogonin D Natural products C[C@@H]1CC[C@@]2(OC1)O[C@H]3C[C@H]4[C@@H]5CC=C6C[C@@H](O)C[C@@H](O[C@@H]7O[C@H](CO)[C@@H](O)[C@H](O[C@@H]8OC[C@@H](O)[C@H](O)[C@H]8O)[C@H]7O[C@@H]9O[C@@H](C)[C@H](O)[C@@H](O)[C@H]9O)[C@]6(C)[C@H]5CC[C@]4(C)[C@H]3[C@@H]2C IDGCVTONMQMXPU-JHZREZMDSA-N 0.000 claims description 7
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 6
- XTGHTMMHUVFPBQ-ZUTFNMMGSA-N liriope muscari baily saponins c Chemical compound O([C@H]1[C@@H](O)[C@@H](C)O[C@H]([C@@H]1O[C@H]1[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O1)O)O[C@@H]1C[C@H](O)CC2=CC[C@H]3[C@@H]4C[C@H]5[C@@H]([C@]4(CC[C@@H]3[C@]21C)C)[C@@H]([C@]1(OC[C@H](C)CC1)O5)C)[C@@H]1OC[C@@H](O)[C@H](O)[C@H]1O XTGHTMMHUVFPBQ-ZUTFNMMGSA-N 0.000 claims description 6
- 238000001694 spray drying Methods 0.000 claims description 5
- 229910021642 ultra pure water Inorganic materials 0.000 claims description 5
- 239000012498 ultrapure water Substances 0.000 claims description 5
- 238000001816 cooling Methods 0.000 claims description 4
- 238000005259 measurement Methods 0.000 claims description 4
- 238000002156 mixing Methods 0.000 claims description 4
- 238000001139 pH measurement Methods 0.000 claims description 4
- 238000002390 rotary evaporation Methods 0.000 claims description 4
- 238000012216 screening Methods 0.000 claims description 4
- 230000007071 enzymatic hydrolysis Effects 0.000 claims description 2
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 claims description 2
- 241000209140 Triticum Species 0.000 claims 1
- 235000021307 Triticum Nutrition 0.000 claims 1
- 230000015556 catabolic process Effects 0.000 claims 1
- 238000006731 degradation reaction Methods 0.000 claims 1
- 235000010987 pectin Nutrition 0.000 claims 1
- 229920001277 pectin Polymers 0.000 claims 1
- 239000001814 pectin Substances 0.000 claims 1
- 241001465754 Metazoa Species 0.000 abstract description 10
- 241000282326 Felis catus Species 0.000 abstract description 3
- 241000283973 Oryctolagus cuniculus Species 0.000 abstract description 3
- 230000003648 hair appearance Effects 0.000 abstract description 2
- 230000037308 hair color Effects 0.000 abstract description 2
- 241000699666 Mus <mouse, genus> Species 0.000 abstract 1
- 241000009328 Perro Species 0.000 abstract 1
- 238000012360 testing method Methods 0.000 description 27
- 238000009835 boiling Methods 0.000 description 18
- 238000004064 recycling Methods 0.000 description 15
- 239000000463 material Substances 0.000 description 13
- 239000000203 mixture Substances 0.000 description 12
- 235000014590 basal diet Nutrition 0.000 description 6
- 239000000110 cooling liquid Substances 0.000 description 6
- 230000002255 enzymatic effect Effects 0.000 description 6
- XYIBRDXRRQCHLP-UHFFFAOYSA-N ethyl acetoacetate Chemical compound CCOC(=O)CC(C)=O XYIBRDXRRQCHLP-UHFFFAOYSA-N 0.000 description 6
- 238000001704 evaporation Methods 0.000 description 6
- 230000008020 evaporation Effects 0.000 description 6
- 238000002525 ultrasonication Methods 0.000 description 6
- 229960004756 ethanol Drugs 0.000 description 5
- 235000019441 ethanol Nutrition 0.000 description 5
- 239000003814 drug Substances 0.000 description 4
- 239000003795 chemical substances by application Substances 0.000 description 3
- 230000000052 comparative effect Effects 0.000 description 3
- 239000002131 composite material Substances 0.000 description 3
- 238000011161 development Methods 0.000 description 3
- 239000003651 drinking water Substances 0.000 description 3
- 235000020188 drinking water Nutrition 0.000 description 3
- 239000002366 mineral element Substances 0.000 description 3
- 239000011782 vitamin Substances 0.000 description 3
- 229940088594 vitamin Drugs 0.000 description 3
- 229930003231 vitamin Natural products 0.000 description 3
- 235000013343 vitamin Nutrition 0.000 description 3
- 150000003722 vitamin derivatives Chemical class 0.000 description 3
- 230000005428 wave function Effects 0.000 description 3
- 244000025254 Cannabis sativa Species 0.000 description 2
- 210000000077 angora Anatomy 0.000 description 2
- 239000008157 edible vegetable oil Substances 0.000 description 2
- 235000013312 flour Nutrition 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 238000011835 investigation Methods 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 235000013372 meat Nutrition 0.000 description 2
- 239000008267 milk Substances 0.000 description 2
- 210000004080 milk Anatomy 0.000 description 2
- 235000013336 milk Nutrition 0.000 description 2
- 244000068988 Glycine max Species 0.000 description 1
- 235000010469 Glycine max Nutrition 0.000 description 1
- 241001532026 Liriope muscari Species 0.000 description 1
- 108010059820 Polygalacturonase Proteins 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- 235000019730 animal feed additive Nutrition 0.000 description 1
- 239000003674 animal food additive Substances 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 229940126678 chinese medicines Drugs 0.000 description 1
- 235000005822 corn Nutrition 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 230000008451 emotion Effects 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 238000003810 ethyl acetate extraction Methods 0.000 description 1
- 108010093305 exopolygalacturonase Proteins 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 230000003814 hair luster Effects 0.000 description 1
- 241000411851 herbal medicine Species 0.000 description 1
- 210000004072 lung Anatomy 0.000 description 1
- 235000012054 meals Nutrition 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 230000000474 nursing effect Effects 0.000 description 1
- 229930190017 ophiopogonin Natural products 0.000 description 1
- 229930190714 ophiopogonone Natural products 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 239000001397 quillaja saponaria molina bark Substances 0.000 description 1
- 238000007788 roughening Methods 0.000 description 1
- 229930182490 saponin Natural products 0.000 description 1
- 235000017709 saponins Nutrition 0.000 description 1
- 150000007949 saponins Chemical class 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 239000002351 wastewater Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/40—Feeding-stuffs specially adapted for particular animals for carnivorous animals, e.g. cats or dogs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
Abstract
The present invention provides a kind of Radix Ophiopogonis feed for pet additive with U.S. hair effect, it is characterised in that: the raw material including following parts by weight mixes: plant extracts 35-150 parts of Radix Ophiopogonis, 40-140 parts of Radix Liriopes plant extracts, bioactive ingredients 0.5-1.5 parts of Radix Ophiopogonis.This additive can be added in pet dog, cat, rabbit, mouse or other feeds to produce economic animal based on fur etc., and can play improves hair quality, the function of hair color.
Description
Technical field
The invention belongs to feed for pet field more particularly to a kind of Radix Ophiopogonis feed for pet additives with U.S. hair effect.
Background technique
In recent years, with the fast development of China's economic, when material and culture reach a certain height, people are sought for spirit
Civilization.And pet is increasingly becoming one kind effectively selection of people's emotion sustenance, it is adjoint while the development relationship with pet
The every profession and trade of pet also rise therewith.Wherein, feed for pet industry is also continuously developed growth.Feed for pet be exclusively for
The food that pet provides, the high-grade animal foodstuff between human food and traditional animal and fowl fodder.
Radix Ophiopogonis and Radix Liriopes are recorded in going through an edition Pharmacopoeia of the People's Republic of China, with nourishing Yin and promoting production of body fluid, moistening lung and clearing away the heart-fire etc.
Function and be widely used, be that China commonly uses large Chinese medicine, and often has one of herbal medicine raw material.This project group membership is in reality
It is found in ground investigation and investigation, Radix Ophiopogonis and Radix Liriopes producing region often have personnel that both Chinese medicines are added to pet or animal feeding-stuff
In, to play the role of anti-depilation and increase hair luster and compliance.This seminar carries out scientific experimentation accordingly, further demonstrate,proves
Radix Ophiopogonis and Radix Liriopes, which is illustrated, has the function of U.S. hair, and not yet finds toxic side effect, can be used as animal feed additive use.
Pet hair quality, hair color are the important indicators of pet kind and aesthetics, however due to nursing, living environment and pet
The problems such as reasons such as owner's living habit, pet hair falls off, fades, is roughening, also highlights.But it is asked at present for above-mentioned
Topic only have medicament smear, wash/externally applied products such as hair rinse are remedied, cannot fundamentally solve.
Summary of the invention
According to the above technical problem, the present invention provides a kind of Radix Ophiopogonis feed for pet additive with U.S. hair effect, special
Sign is: the raw material including following parts by weight mixes:
Plant extracts 35-150 parts of Radix Ophiopogonis, 40-140 parts of Radix Liriopes plant extracts, Radix Ophiopogonis bioactive ingredients 0.5-
1.5 part.
The Radix Ophiopogonis plant extracts the preparation method comprises the following steps:
A. it will be crushed after the drying of Radix Ophiopogonis stem tuber, 150 mesh standard sieves crossed after crushing, are added to powder after screening
In the water of 25-30 times of volume, ultrasonic wave extraction is then carried out in mixing liquid, acoustic power used in the ultrasonic wave extraction is
200W-500W, ultrasonic temperature are 30-60 DEG C, and the ultrasonic wave extraction time is 10-60min；To liquid after ultrasonic wave extraction
PH measurement is carried out, PH adjusting is carried out after measurement, adjusts and arrives 3-6, then at 30-60 DEG C of temperature, according to certain enzyme sequence,
It is separately added into cellulase, pectase and papain, digests 40-100min, the extracting solution filtered after cooling will mention
After taking liquid to be concentrated in vacuo to the 30% of original volume, centrifugation obtains supernatant and sediment, takes supernatant with isometric ethyl acetate
Extraction 3 times, recycles aqueous phase liquid, combined ethyl acetate phase liquid, and rotary evaporation recycles ethyl acetate, obtains Radix Ophiopogonis ethyl acetate
Extract；
B. the aqueous phase liquid recycled after extracting in a is taken, after being concentrated in vacuo to the 30% of original volume, centrifugation obtains supernatant
Liquid and sediment take supernatant that the dehydrated alcohol of 5 times of volumes is added, stand overnight, sediment is obtained after filtering, successively with anhydrous
Ethyl alcohol, acetone washing precipitate 3 times, will precipitate vacuum freeze-drying, obtain Radix Ophiopogonis water extract；
C. Radix Ophiopogonis ethyl acetate extract and Radix Ophiopogonis water extract are uniformly mixed, obtain Radix Ophiopogonis plant extracts.
The Radix Liriopes plant extracts the preparation method comprises the following steps:
A. it will be crushed after the drying of Radix Liriopes stem tuber, 150 mesh standard sieves crossed after crushing, powder is added to 25- after screening
In the water of 30 times of volumes, ultrasonic wave extraction, ultrasonic power used in the ultrasonic wave extraction are then carried out in mixing liquid
200W-500W, 30-60 DEG C of ultrasonic temperature, ultrasonic wave extraction 10-60min；PH measurement is carried out to liquid after ultrasonic wave extraction,
PH is adjusted to 3-6 using NaOH or HCl solution after measurement, 30-60 DEG C of temperature, according to certain enzyme sequence, the fibre of addition
Plain enzyme, pectase and papain are tieed up, 40-100min is digested, the extracting solution filtered after cooling is dense by extracting solution vacuum
After being reduced to the 30% of original volume, centrifugation obtains supernatant and sediment, and supernatant is taken to be extracted 3 times with isometric ethyl acetate, returns
Aqueous phase liquid, combined ethyl acetate phase liquid are received, rotary evaporation recycles ethyl acetate, obtains Radix Liriopes ethyl acetate extract；
B. the aqueous phase liquid recycled after extracting in a is taken, after being concentrated in vacuo to the 30% of original volume, centrifugation obtains supernatant
Liquid and sediment take supernatant that the dehydrated alcohol of 5 times of volumes is added, stand overnight, sediment is obtained after filtering, successively with anhydrous
Ethyl alcohol, acetone washing precipitate 3 times, will precipitate vacuum freeze-drying, obtain Radix Liriopes water extract；
C. Radix Ophiopogonis ethyl acetate extract and Radix Ophiopogonis water extract are uniformly mixed, obtain Radix Liriopes plant extracts.
The Radix Ophiopogonis bioactive ingredients the preparation method comprises the following steps:
Take equivalent Ophiopogonin D powder, Liriope muscaribaily saponins C powder and
MethylophiopogonanoneA powder is dissolved in ultrapure water, then is spray-dried, spray drying EAT 160
DEG C, leaving air temp is 110 DEG C, obtains Radix Ophiopogonis bioactive ingredients after dry.
The enzyme sequence are as follows: pectase → cellulase → papain, the enzyme concentration of the enzyme are 0.4-
1.3%, the condition of pectinase enzymatic hydrolysis are as follows: pH 3.4,52 DEG C of temperature, time 34min, enzyme concentration 0.42%；Cellulose enzyme
The condition of solution are as follows: pH 3.8,34 DEG C of temperature, time 37min, enzyme concentration 0.47%；The condition of papain enzymolysis are as follows: pH
5.7,35 DEG C of temperature, time 51min, enzyme concentration 0.54%.
The adding proportion of the Radix Ophiopogonis feed for pet additive is the 0.3-1.5% of premix or batch total weight.
The present invention uses Chinese medicine Radix Ophiopogonis, Radix Liriopes and its extract for raw material, and can fundamentally solve animal U.S. hair needs
It asks, market prospects are considerable, can play facilitation for Radix Ophiopogonis, Radix Liriopes industry development.Equipment needed for the present invention is simple, drug valence
Lattice are cheap, and quickly operation, quick income may be implemented.Preparation section of the present invention is simple, the production time is short, and has and generate waste water
Less, the features such as environmental pollution is small.The present invention is rich in bioactive ingredients such as polysaccharide, ophiopogonin class and ophiopogonone classes, right
While animal U.S. hair, the effects of adjusting is immune, promotion is grown may also function as.Radix Ophiopogonis feed for pet additive of the present invention can
It is added in pet or the premix of economic animal routine or batch, therefore, the present invention also provides raise containing the Radix Ophiopogonis pet
The premix of feed additives or batch.
Specific embodiment
According to embodiment, invention is further explained:
Ophiopogonin D is ophiopogonin D, Liriope muscaribaily saponins C is liriope muscari Baily
Saponin(e C, Methylophiopogonanone A are Methylophiopogonanone A B.
Embodiment 1:
It carries out Radix Ophiopogonis plant extracts, Radix Liriopes plant extracts, Radix Ophiopogonis bioactive ingredients to be prepared, Radix Ophiopogonis plant
Extract the preparation method comprises the following steps:
A. it will be crushed after the drying of Radix Ophiopogonis stem tuber, cross 150 mesh standard sieves, be added in the water of 25-30 times of volume, in ultrasonic wave function
Rate 300W, under the conditions of 55 DEG C of ultrasonic temperature, ultrasonic wave extraction 43min；According to pectase → cellulase → papain
Enzyme sequence to after ultrasonication material liquid mixture carry out Enzymatic Extraction.PH to 3.4 is adjusted, 52 DEG C of temperature, is added
0.42% cellulase digests 34min.5min boiling water bath after enzyme-deactivating, will adjust pH to 3.8,34 DEG C of temperature, be added
0.47% cellulase digests 37min.5min boiling water bath after enzyme-deactivating, will adjust pH to 5.7,35 DEG C of temperature, be added
0.54% papain enzymolysis 51min, 5min boiling water bath is by enzyme-deactivating.The extraction filtered after material liquid mixture is cooling
Liquid.After extracting solution to be concentrated in vacuo to the 30% of original volume, centrifugation obtains supernatant and sediment.Take supernatant with isometric second
Acetoacetic ester extracts 3 times, merges recycling aqueous phase liquid, combined ethyl acetate phase liquid.The evaporation recycling of ethyl acetate phase liquid in rotation
Ethyl acetate obtains Radix Ophiopogonis ethyl acetate extract.
B. the aqueous phase liquid for merging recycling after extracting in a is taken, after being concentrated in vacuo to the 30% of original volume, centrifugation is obtained
Supernatant and sediment.It takes supernatant that the dehydrated alcohol of 5 times of volumes is added, stands overnight, obtain sediment after filtering.Successively use
Dehydrated alcohol, acetone washing precipitate 3 times, will precipitate vacuum freeze-drying, obtain Radix Ophiopogonis water extract.
C. Radix Ophiopogonis ethyl acetate extract and Radix Ophiopogonis water extract are uniformly mixed, obtain Radix Ophiopogonis plant extracts.
Radix Liriopes plant extracts the preparation method comprises the following steps:
A. it will be crushed after the drying of Radix Liriopes stem tuber, cross 150 mesh standard sieves, be added in the water of 25-30 times of volume, in ultrasonic wave
Power 300W, under the conditions of 55 DEG C of ultrasonic temperature, ultrasonic wave extraction 43min；According to pectase → cellulase → Papain
The enzyme sequence of enzyme carries out Enzymatic Extraction to the material liquid mixture after ultrasonication.PH to 3.4 is adjusted, 52 DEG C of temperature, is added
0.42% cellulase digests 34min.5min boiling water bath after enzyme-deactivating, will adjust pH to 3.8,34 DEG C of temperature, be added
0.47% cellulase digests 37min.5min boiling water bath after enzyme-deactivating, will adjust pH to 5.7,35 DEG C of temperature, be added
0.54% papain enzymolysis 51min, 5min boiling water bath is by enzyme-deactivating.The extraction filtered after material liquid mixture is cooling
Liquid.After extracting solution to be concentrated in vacuo to the 30% of original volume, centrifugation obtains supernatant and sediment.Take supernatant with isometric second
Acetoacetic ester extracts 3 times, merges recycling aqueous phase liquid, combined ethyl acetate phase liquid.The evaporation recycling of ethyl acetate phase liquid in rotation
Ethyl acetate obtains Radix Liriopes ethyl acetate extract.
B. the aqueous phase liquid recycled after extracting in a is taken, after being concentrated in vacuo to the 30% of original volume, centrifugation obtains supernatant
Liquid and sediment.It takes supernatant that the dehydrated alcohol of 5 times of volumes is added, stands overnight, obtain sediment after filtering.Successively with anhydrous
Ethyl alcohol, acetone washing precipitate 3 times, will precipitate vacuum freeze-drying, obtain Radix Liriopes water extract.
C. Radix Ophiopogonis ethyl acetate extract and Radix Ophiopogonis water extract are uniformly mixed, obtain Radix Liriopes plant extracts.
Radix Ophiopogonis bioactive ingredients the preparation method comprises the following steps:
Take equivalent Ophiopogonin D powder, Liriope muscaribaily saponins C powder and
MethylophiopogonanoneA powder is dissolved in ultrapure water, then is spray-dried, spray drying EAT 160
DEG C, leaving air temp is 110 DEG C, obtains Radix Ophiopogonis bioactive ingredients after dry.
Choose the above-mentioned raw material produced, by Radix Ophiopogonis plant extracts, Radix Liriopes plant extracts, Radix Ophiopogonis bioactivity at
Divide and chosen according to the 136: 123: 1 of part, is mixed after selection, additive completes.It will system when use
The additive for making to complete is put into feed for pet, and adding the adding proportion of agent is the 0.5% of feed total amount.
Embodiment 2:
It carries out Radix Ophiopogonis plant extracts, Radix Liriopes plant extracts, Radix Ophiopogonis bioactive ingredients to be prepared, Radix Ophiopogonis plant
Extract the preparation method comprises the following steps:
A. it will be crushed after the drying of Radix Ophiopogonis stem tuber, cross 150 mesh standard sieves, be added in the water of 25-30 times of volume, in ultrasonic wave function
Rate 300W, under the conditions of 55 DEG C of ultrasonic temperature, ultrasonic wave extraction 43min；According to pectase → cellulase → papain
Enzyme sequence to after ultrasonication material liquid mixture carry out Enzymatic Extraction.PH to 3.4 is adjusted, 52 DEG C of temperature, is added
0.42% cellulase digests 34min.5min boiling water bath after enzyme-deactivating, will adjust pH to 3.8,34 DEG C of temperature, be added
0.47% cellulase digests 37min.5min boiling water bath after enzyme-deactivating, will adjust pH to 5.7,35 DEG C of temperature, be added
0.54% papain enzymolysis 51min, 5min boiling water bath is by enzyme-deactivating.The extraction filtered after material liquid mixture is cooling
Liquid.After extracting solution to be concentrated in vacuo to the 30% of original volume, centrifugation obtains supernatant and sediment.Take supernatant with isometric second
Acetoacetic ester extracts 3 times, merges recycling aqueous phase liquid, combined ethyl acetate phase liquid.The evaporation recycling of ethyl acetate phase liquid in rotation
Ethyl acetate obtains Radix Ophiopogonis ethyl acetate extract.
B. the aqueous phase liquid for merging recycling after extracting in a is taken, after being concentrated in vacuo to the 30% of original volume, centrifugation is obtained
Supernatant and sediment.It takes supernatant that the dehydrated alcohol of 5 times of volumes is added, stands overnight, obtain sediment after filtering.Successively use
Dehydrated alcohol, acetone washing precipitate 3 times, will precipitate vacuum freeze-drying, obtain Radix Ophiopogonis water extract.
C. Radix Ophiopogonis ethyl acetate extract and Radix Ophiopogonis water extract are uniformly mixed, obtain Radix Ophiopogonis plant extracts.
Radix Liriopes plant extracts the preparation method comprises the following steps:
A. it will be crushed after the drying of Radix Liriopes stem tuber, cross 150 mesh standard sieves, be added in the water of 25-30 times of volume, in ultrasonic wave
Power 300W, under the conditions of 55 DEG C of ultrasonic temperature, ultrasonic wave extraction 43min；According to pectase → cellulase → Papain
The enzyme sequence of enzyme carries out Enzymatic Extraction to the material liquid mixture after ultrasonication.PH to 3.4 is adjusted, 52 DEG C of temperature, is added
0.42% cellulase digests 34min.5min boiling water bath after enzyme-deactivating, will adjust pH to 3.8,34 DEG C of temperature, be added
0.47% cellulase digests 37min.5min boiling water bath after enzyme-deactivating, will adjust pH to 5.7,35 DEG C of temperature, be added
0.54% papain enzymolysis 51min, 5min boiling water bath is by enzyme-deactivating.The extraction filtered after material liquid mixture is cooling
Liquid.After extracting solution to be concentrated in vacuo to the 30% of original volume, centrifugation obtains supernatant and sediment.Take supernatant with isometric second
Acetoacetic ester extracts 3 times, merges recycling aqueous phase liquid, combined ethyl acetate phase liquid.The evaporation recycling of ethyl acetate phase liquid in rotation
Ethyl acetate obtains Radix Liriopes ethyl acetate extract.
B. the aqueous phase liquid recycled after extracting in a is taken, after being concentrated in vacuo to the 30% of original volume, centrifugation obtains supernatant
Liquid and sediment.It takes supernatant that the dehydrated alcohol of 5 times of volumes is added, stands overnight, obtain sediment after filtering.Successively with anhydrous
Ethyl alcohol, acetone washing precipitate 3 times, will precipitate vacuum freeze-drying, obtain Radix Liriopes water extract.
C. Radix Ophiopogonis ethyl acetate extract and Radix Ophiopogonis water extract are uniformly mixed, obtain Radix Liriopes plant extracts.
Radix Ophiopogonis bioactive ingredients the preparation method comprises the following steps:
Take equivalent Ophiopogonin D powder, Liriope muscaribaily saponins C powder and
Methylophiopogonanone A powder is dissolved in ultrapure water, then is spray-dried, spray drying EAT 160
DEG C, leaving air temp is 110 DEG C, obtains Radix Ophiopogonis bioactive ingredients after dry.
Choose the above-mentioned raw material produced, by Radix Ophiopogonis plant extracts, Radix Liriopes plant extracts, Radix Ophiopogonis bioactivity at
Divide and chosen according to the 50: 100: 0.6 of part, is mixed after selection, additive completes.It will system when use
The additive for making to complete is put into feed for pet, and adding the adding proportion of agent is the 1% of feed total amount.
Embodiment 3:
It carries out Radix Ophiopogonis plant extracts, Radix Liriopes plant extracts, Radix Ophiopogonis bioactive ingredients to be prepared, Radix Ophiopogonis plant
Extract the preparation method comprises the following steps:
A. it will be crushed after the drying of Radix Ophiopogonis stem tuber, cross 150 mesh standard sieves, be added in the water of 25-30 times of volume, in ultrasonic wave function
Rate 300W, under the conditions of 55 DEG C of ultrasonic temperature, ultrasonic wave extraction 43min；According to pectase → cellulase → papain
Enzyme sequence to after ultrasonication material liquid mixture carry out Enzymatic Extraction.PH to 3.4 is adjusted, 52 DEG C of temperature, is added
0.42% cellulase digests 34min.5min boiling water bath after enzyme-deactivating, will adjust pH to 3.8,34 DEG C of temperature, be added
0.47% cellulase digests 37min.5min boiling water bath after enzyme-deactivating, will adjust pH to 5.7,35 DEG C of temperature, be added
0.54% papain enzymolysis 51min, 5min boiling water bath is by enzyme-deactivating.The extraction filtered after material liquid mixture is cooling
Liquid.After extracting solution to be concentrated in vacuo to the 30% of original volume, centrifugation obtains supernatant and sediment.Take supernatant with isometric second
Acetoacetic ester extracts 3 times, merges recycling aqueous phase liquid, combined ethyl acetate phase liquid.The evaporation recycling of ethyl acetate phase liquid in rotation
Ethyl acetate obtains Radix Ophiopogonis ethyl acetate extract.
B. the aqueous phase liquid for merging recycling after extracting in a is taken, after being concentrated in vacuo to the 30% of original volume, centrifugation is obtained
Supernatant and sediment.It takes supernatant that the dehydrated alcohol of 5 times of volumes is added, stands overnight, obtain sediment after filtering.Successively use
Dehydrated alcohol, acetone washing precipitate 3 times, will precipitate vacuum freeze-drying, obtain Radix Ophiopogonis water extract.
C. Radix Ophiopogonis ethyl acetate extract and Radix Ophiopogonis water extract are uniformly mixed, obtain Radix Ophiopogonis plant extracts.
Radix Liriopes plant extracts the preparation method comprises the following steps:
A. it will be crushed after the drying of Radix Liriopes stem tuber, cross 150 mesh standard sieves, be added in the water of 25-30 times of volume, in ultrasonic wave
Power 300W, under the conditions of 55 DEG C of ultrasonic temperature, ultrasonic wave extraction 43min；According to pectase → cellulase → Papain
The enzyme sequence of enzyme carries out Enzymatic Extraction to the material liquid mixture after ultrasonication.PH to 3.4 is adjusted, 52 DEG C of temperature, is added
0.42% cellulase digests 34min.5min boiling water bath after enzyme-deactivating, will adjust pH to 3.8,34 DEG C of temperature, be added
0.47% cellulase digests 37min.5min boiling water bath after enzyme-deactivating, will adjust pH to 5.7,35 DEG C of temperature, be added
0.54% papain enzymolysis 51min, 5min boiling water bath is by enzyme-deactivating.The extraction filtered after material liquid mixture is cooling
Liquid.After extracting solution to be concentrated in vacuo to the 30% of original volume, centrifugation obtains supernatant and sediment.Take supernatant with isometric second
Acetoacetic ester extracts 3 times, merges recycling aqueous phase liquid, combined ethyl acetate phase liquid.The evaporation recycling of ethyl acetate phase liquid in rotation
Ethyl acetate obtains Radix Liriopes ethyl acetate extract.
B. the aqueous phase liquid recycled after extracting in a is taken, after being concentrated in vacuo to the 30% of original volume, centrifugation obtains supernatant
Liquid and sediment.It takes supernatant that the dehydrated alcohol of 5 times of volumes is added, stands overnight, obtain sediment after filtering.Successively with anhydrous
Ethyl alcohol, acetone washing precipitate 3 times, will precipitate vacuum freeze-drying, obtain Radix Liriopes water extract.
C. Radix Ophiopogonis ethyl acetate extract and Radix Ophiopogonis water extract are uniformly mixed, obtain Radix Liriopes plant extracts.
Radix Ophiopogonis bioactive ingredients the preparation method comprises the following steps:
Take equivalent Ophiopogonin D powder, Liriope muscaribaily saponins C powder and
MethylophiopogonanoneA powder is dissolved in ultrapure water, then is spray-dried, spray drying EAT 160
DEG C, leaving air temp is 110 DEG C, obtains Radix Ophiopogonis bioactive ingredients after dry.
Choose the above-mentioned raw material produced, by Radix Ophiopogonis plant extracts, Radix Liriopes plant extracts, Radix Ophiopogonis bioactivity at
Divide and chosen according to the 100: 80: 1.2 of part, is mixed after selection, additive completes.It will system when use
The additive for making to complete is put into feed for pet, and adding the adding proportion of agent is the 1.4% of feed total amount.
Embodiment 4
The present invention has carried out comparative test to pet dog, selects the 12 more than monthly age safe enlightening dog 100 of bouncing, according to
Weight and sex ratio phase approximately principle are divided into 4 groups, respectively control group, test group 1, test group 2, test group 3.Control group is fed
Basal diet, test group 1 feed the Radix Ophiopogonis feed for pet additive that addition embodiment 1 describes, and test group 2 feeds addition embodiment
The Radix Ophiopogonis feed for pet additive of 2 descriptions, test group 3 feed the Radix Ophiopogonis feed for pet additive that addition embodiment 3 describes.Daily
It is consistent to feed feed total weight, test feed period is 60 days.The formula of basal diet are as follows: meat 250g, milk powder 150g, edible oil
50g, flour 300g, mineral element and vitamin composite sheet 1.For the examination animal free-ranging under same environment, can move freely,
Drinking-water, three times a day feeds.Effect is shown in Table 1.
1 control group of table and test group U.S. hair and anti-nose fade results
Embodiment 5
The present invention has carried out comparative test to pet cat, selects 12 monthly ages of the bouncing above Persian cat 100, according to
Weight and sex ratio phase approximately principle are divided into 4 groups, respectively control group, test group 1, test group 2, test group 3.Control group is fed
Basal diet, test group 1 feed the Radix Ophiopogonis feed for pet additive that addition embodiment 1 describes, and test group 2 feeds addition embodiment
The Radix Ophiopogonis feed for pet additive of 2 descriptions, test group 3 feed the Radix Ophiopogonis feed for pet additive that addition embodiment 3 describes.Daily
It is consistent to feed feed total weight, test feed period is 60 days.The formula of basal diet are as follows: meat 200g, milk powder 200g, edible oil
30g, flour 250g, mineral element and vitamin composite sheet 1.For the examination animal free-ranging under same environment, can move freely,
Drinking-water, three times a day feeds.Effect is shown in Table 2.
2 control group of table and test group U.S. hair and anti-nose fade results
Embodiment 6
The present invention has carried out comparative test to Angora rabbit, selects 6 monthly ages of the bouncing above Angora rabbit 100,
It is divided into 4 groups, respectively control group, test group 1, test group 2, test group 3 according to weight and sex ratio phase approximately principle.Control group
Basal diet is fed, test group 1 feeds the Radix Ophiopogonis feed for pet additive that addition embodiment 1 describes, and it is real that test group 2 feeds addition
The Radix Ophiopogonis feed for pet additive of the description of example 2 is applied, test group 3 feeds the Radix Ophiopogonis feed for pet additive that addition embodiment 3 describes.
It is consistent that feed total weight is fed daily, and test feed period is 60 days.The formula of basal diet are as follows: corn 25g, soya-bean cake 40g, grass meal
50g, mineral element and vitamin composite sheet 0.2 separately feed fresh grass 800g.It, can be certainly for trying the animal free-ranging under same environment
By activity, drinking-water, three times a day feed.Effect is shown in Table 3.
3 control group of table and test group U.S. hair and anti-nose fade results
The above is only a preferred embodiment of the present invention, it is noted that for the common skill of the art
For art personnel, without departing from the principle of the present invention, several improvement can also be made, these improvement also should be regarded as this hair
Bright protection scope.
Claims (6)
1. a kind of Radix Ophiopogonis feed for pet additive with U.S. hair effect, it is characterised in that: the raw material including following parts by weight is mixed
It closes:
Plant extracts 35-150 parts of Radix Ophiopogonis, 40-140 parts of Radix Liriopes plant extracts, Radix Ophiopogonis bioactive ingredients 0.5-1.5
Part.
2. a kind of Radix Ophiopogonis feed for pet additive with U.S. hair effect described in accordance with the claim 1, it is characterised in that: described
Radix Ophiopogonis plant extracts the preparation method comprises the following steps:
A. it will be crushed after the drying of Radix Ophiopogonis stem tuber, 150 mesh standard sieves crossed after crushing, powder is added to 25-30 after screening
In the water of times volume, ultrasonic wave extraction is then carried out in mixing liquid, acoustic power used in the ultrasonic wave extraction is 200W-
500W, ultrasonic temperature are 30-60 DEG C, and the ultrasonic wave extraction time is 10-60min；Liquid is carried out after ultrasonic wave extraction
PH measurement, carries out PH adjusting after measurement, adjust and arrive 3-6, then at 30-60 DEG C of temperature, according to certain enzyme sequence, respectively
Cellulase, pectase and papain is added, digests 40-100min, the extracting solution filtered after cooling, by extracting solution
After being concentrated in vacuo to the 30% of original volume, centrifugation obtains supernatant and sediment, and supernatant is taken to extract 3 with isometric ethyl acetate
It is secondary, aqueous phase liquid, combined ethyl acetate phase liquid are recycled, rotary evaporation recycles ethyl acetate, obtains the extraction of Radix Ophiopogonis ethyl acetate
Object；
B. take the aqueous phase liquid that recycles after extracting in a, after being concentrated in vacuo to the 30% of original volume, centrifugation obtain supernatant with
Sediment takes supernatant that the dehydrated alcohol of 5 times of volumes is added, stands overnight, sediment is obtained after filtering, successively with anhydrous second
Alcohol, acetone washing precipitate 3 times, will precipitate vacuum freeze-drying, obtain Radix Ophiopogonis water extract；
C. Radix Ophiopogonis ethyl acetate extract and Radix Ophiopogonis water extract are uniformly mixed, obtain Radix Ophiopogonis plant extracts.
3. a kind of Radix Ophiopogonis feed for pet additive with U.S. hair effect described in accordance with the claim 1, it is characterised in that: described
Radix Liriopes plant extracts the preparation method comprises the following steps:
A. it will be crushed after the drying of Radix Liriopes stem tuber, 150 mesh standard sieves crossed after crushing, powder is added to 25-30 times after screening
In the water of volume, ultrasonic wave extraction is then carried out in mixing liquid, ultrasonic power 200W- used in the ultrasonic wave extraction
500W, 30-60 DEG C of ultrasonic temperature, ultrasonic wave extraction 10-60min；PH measurement is carried out to liquid after ultrasonic wave extraction, after measurement
PH is adjusted to 3-6 using NaOH or HCl solution, 30-60 DEG C of temperature, according to certain enzyme sequence, the cellulase of addition,
Pectase and papain, digest 40-100min, and extracting solution is concentrated in vacuo to original by the extracting solution filtered after cooling
After the 30% of volume, centrifugation obtains supernatant and sediment, and supernatant is taken to be extracted 3 times with isometric ethyl acetate, recycles water phase
Liquid, combined ethyl acetate phase liquid, rotary evaporation recycle ethyl acetate, obtain Radix Liriopes ethyl acetate extract；
B. take the aqueous phase liquid that recycles after extracting in a, after being concentrated in vacuo to the 30% of original volume, centrifugation obtain supernatant with
Sediment takes supernatant that the dehydrated alcohol of 5 times of volumes is added, stands overnight, sediment is obtained after filtering, successively with anhydrous second
Alcohol, acetone washing precipitate 3 times, will precipitate vacuum freeze-drying, obtain Radix Liriopes water extract；
C. Radix Ophiopogonis ethyl acetate extract and Radix Ophiopogonis water extract are uniformly mixed, obtain Radix Liriopes plant extracts.
4. a kind of Radix Ophiopogonis feed for pet additive with U.S. hair effect described in accordance with the claim 1, it is characterised in that: described
Radix Ophiopogonis bioactive ingredients the preparation method comprises the following steps:
Take equivalent Ophiopogonin D powder, Liriope muscaribaily saponins C powder and
Methylophiopogonanone A powder is dissolved in ultrapure water, then is spray-dried, spray drying EAT 160
DEG C, leaving air temp is 110 DEG C, obtains Radix Ophiopogonis bioactive ingredients after dry.
5. existing according to a kind of Radix Ophiopogonis feed for pet additive with U.S. hair effect, feature described in claim 2,3 or 4
In: the enzyme sequence are as follows: pectase → cellulase → papain, the enzyme concentration of the enzyme are 0.4-1.3%, pectin
The condition of enzyme enzymatic hydrolysis are as follows: pH 3.4,52 DEG C of temperature, time 34min, enzyme concentration 0.42%；The condition of cellulase degradation are as follows:
PH 3.8,34 DEG C of temperature, time 37min, enzyme concentration 0.47%；The condition of papain enzymolysis are as follows: pH 5.7, temperature 35
DEG C, time 51min, enzyme concentration 0.54%.
6. a kind of Radix Ophiopogonis feed for pet additive with U.S. hair effect described in accordance with the claim 1, is characterized in that: the wheat
The adding proportion of winter feed for pet additive is the 0.3-1.5% of premix or batch total weight.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2017111671751 | 2017-11-21 | ||
| CN201711167175 | 2017-11-21 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN109007298A true CN109007298A (en) | 2018-12-18 |
| CN109007298B CN109007298B (en) | 2021-08-24 |
Family
ID=64640499
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810736697.7A Active CN109007298B (en) | 2017-11-21 | 2018-07-06 | Ophiopogon japonicus pet feed additive with hair beautifying effect |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109007298B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111296635A (en) * | 2019-12-16 | 2020-06-19 | 郑州立蓓生物工程有限公司 | Pet hair-beautifying food and preparation method thereof |
| CN114177257A (en) * | 2021-07-22 | 2022-03-15 | 山东畜牧兽医职业学院 | Traditional Chinese medicine composition for brightening skin and hair of pets and preparation thereof |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20050238741A1 (en) * | 2002-03-29 | 2005-10-27 | Kim Sung-Jin | Composition comprising an extract of liriopsis tuber for protecting brain cells and improving memory |
| CN102127042A (en) * | 2011-01-26 | 2011-07-20 | 浙江大学 | Homoisoflavonoid compounds and preparation method and use thereof |
| CN102406833A (en) * | 2010-12-01 | 2012-04-11 | 成都中医药大学 | Comprehensive extraction and utilization technology for creeping liriope |
| CN103494037A (en) * | 2013-09-30 | 2014-01-08 | 山东省健牧生物药业有限公司 | Fur-bearing animal feed additive |
| CN105639149A (en) * | 2015-12-29 | 2016-06-08 | 青岛北斗星云通信科技有限公司 | Pet food with fur brightening and beautifying function |
| CN106615876A (en) * | 2016-12-23 | 2017-05-10 | 安徽赛澳生物工程有限公司 | Hair beautifying feed for pet dogs |
-
2018
- 2018-07-06 CN CN201810736697.7A patent/CN109007298B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20050238741A1 (en) * | 2002-03-29 | 2005-10-27 | Kim Sung-Jin | Composition comprising an extract of liriopsis tuber for protecting brain cells and improving memory |
| CN102406833A (en) * | 2010-12-01 | 2012-04-11 | 成都中医药大学 | Comprehensive extraction and utilization technology for creeping liriope |
| CN102127042A (en) * | 2011-01-26 | 2011-07-20 | 浙江大学 | Homoisoflavonoid compounds and preparation method and use thereof |
| CN103494037A (en) * | 2013-09-30 | 2014-01-08 | 山东省健牧生物药业有限公司 | Fur-bearing animal feed additive |
| CN105639149A (en) * | 2015-12-29 | 2016-06-08 | 青岛北斗星云通信科技有限公司 | Pet food with fur brightening and beautifying function |
| CN106615876A (en) * | 2016-12-23 | 2017-05-10 | 安徽赛澳生物工程有限公司 | Hair beautifying feed for pet dogs |
Non-Patent Citations (1)
| Title |
|---|
| 孙健等: "山麦冬的化学成分及药理作用研究进展", 《中国药物警戒》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111296635A (en) * | 2019-12-16 | 2020-06-19 | 郑州立蓓生物工程有限公司 | Pet hair-beautifying food and preparation method thereof |
| CN114177257A (en) * | 2021-07-22 | 2022-03-15 | 山东畜牧兽医职业学院 | Traditional Chinese medicine composition for brightening skin and hair of pets and preparation thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN109007298B (en) | 2021-08-24 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101438764B (en) | Organic composite biological feed and method for producing the same | |
| CN102204638B (en) | Chinese medicinal feed for fattening pigs | |
| CN104041663A (en) | Mixed feed for feeding tenebrio molitor larva | |
| CN104782957A (en) | Fodder for lambs | |
| CN106614306B (en) | A kind of chicken method for breeding | |
| CN105941833A (en) | Feed capable of improving immunity of eleutheronema tetradactylum and preparation method of feed | |
| CN105494933A (en) | Method for producing bamboo leaf feed additive | |
| CN109007298A (en) | A kind of Radix Ophiopogonis feed for pet additive with U.S. hair effect | |
| CN104855711A (en) | Good-palatability feed for juvenile soft-shelled turtles | |
| CN103892052A (en) | Cordyceps militaris medium immunopotentiator for feeding and production method of immunopotentiator | |
| CN103766660A (en) | Pig feed containing no antibiotics or other synthetic drugs and preparation method of pig feed | |
| CN105995241A (en) | Feed for grass carp culture | |
| CN104705490A (en) | Chinese herbal medicine feed additive for herbivorous animals and application of feed additive | |
| CN108576481A (en) | A kind of egg feedstuff and preparation method thereof | |
| CN104256140A (en) | Feed for yellow meal worm larva and preparation method thereof | |
| CN104187140A (en) | Method for using Chinese herbal medicine duck feed | |
| KR20190014386A (en) | Poultry feed additive for immunity enhancement using korea herbal medicine byproduct and fermented red ginseng red ginseng residue | |
| CN111000032A (en) | Feed additive containing sweet wormwood herb and preparation method and application thereof | |
| CN104782958A (en) | Preparation method of fodder for lambs | |
| CN104256141A (en) | Feed for yellow meal worms and preparation method thereof | |
| CN104839473A (en) | Environmental-friendly chicken feed capable of increasing egg yield and improving egg quality, and preparation method thereof | |
| CN107889967A (en) | Mixed feed during Egg Production of Laying Hens | |
| CN107691875A (en) | Laying-hen compound feed | |
| CN106343190A (en) | Method for producing pig feed from sugar mill alcohol slops | |
| CN107259070A (en) | Poultry feed and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20240116 Address after: 266000 no.778, Mingyue Road, Huangdao District, Qingdao City, Shandong Province Patentee after: Qingdao Mingyue Anxin Nutrition Technology Co.,Ltd. Address before: Room 524, 5th Floor, Unit 2, Building 4, Dexin Yunchuan Business Center, Xihu District, Hangzhou City, Zhejiang Province, 310000 (self declared) Patentee before: Hangzhou Xiyin Information Technology Co.,Ltd. Effective date of registration: 20240116 Address after: Room 524, 5th Floor, Unit 2, Building 4, Dexin Yunchuan Business Center, Xihu District, Hangzhou City, Zhejiang Province, 310000 (self declared) Patentee after: Hangzhou Xiyin Information Technology Co.,Ltd. Address before: 621000 Songya Town, Youxian District, Mianyang City, Sichuan Province Patentee before: MIANYANG ACEDEMY OF AGRICULTURE SCIENCES |