CN109007241A - A method of producing gluten - Google Patents
A method of producing gluten Download PDFInfo
- Publication number
- CN109007241A CN109007241A CN201810761593.1A CN201810761593A CN109007241A CN 109007241 A CN109007241 A CN 109007241A CN 201810761593 A CN201810761593 A CN 201810761593A CN 109007241 A CN109007241 A CN 109007241A
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- Prior art keywords
- filtrate
- supernatant
- gluten
- sediment
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Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/14—Vegetable proteins
- A23J3/18—Vegetable proteins from wheat
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/30—Working-up of proteins for foodstuffs by hydrolysis
- A23J3/32—Working-up of proteins for foodstuffs by hydrolysis using chemical agents
- A23J3/34—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes
- A23J3/346—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes of vegetable proteins
Abstract
The present invention relates to Gluten technical fields, specifically disclose a kind of method for producing Gluten, include the following steps: S1, mix wheat flour with PBS buffer solution, and complex enzyme is added, and are protected from light water-bath extraction, and filtering obtains filtrate a；S2, alkaline solution is added into filtrate a, carries out alkali carries, alkali extract is subjected to centrifugal treating, obtains supernatant a and sediment a；S3, ethanol solution is added into sediment a, carries out alcohol extracting, alcohol extract is subjected to centrifugal treating, obtains supernatant b；S4, supernatant a and supernatant b is mixed, adjusts pH, staticly settles, filter to obtain screening and filtrate b；Filtrate b is subjected to centrifugal treating, obtains sediment b；S5, merge screening and sediment b, add water to be tuned into feed liquid, feed liquid is spray-dried, obtain Gluten.Gluten yield of the invention is up to 60% or more, and protein content in Gluten is up to 92% or more.
Description
Technical field
The present invention relates to Gluten technical fields, and in particular to a method of produce Gluten.
Background technique
Gluten main constituents are glutenin and alcohol soluble protein, in addition also contain a small amount of starch, fat, minerals
Deng.Glutenin is to act on the albumen being polymerized by intermolecular disulfide bond by many peptide bonds, and molecular weight is big, is in threadiness,
Height intersection links together in a manner of branch-like, irregular structure, and it is more that molecule includes beta sheet structure, is rich in glutamine
And cystine.Alcohol soluble protein is monomeric protein, molecular weight about 35kD, spherical in shape, not soluble in water and dehydrated alcohol, but is dissolved in 70-
In 80% ethanol solution, feature is that proline and amide are more, and non-polar sidechain is more compared with polar side chain, and intramolecular is both without subunit
Structure, and without peptide interchain disulfide bond, by hydrogen bond, hydrophobic bond and intramolecular disulfide bond connection between single chain polypeptide, formed closer
Three-dimensional structure.But it is lower now for the recovery rate of Gluten, it is unfavorable for improving the performance of enterprises.
Summary of the invention
Technical problems based on background technology, the invention proposes a kind of methods for producing Gluten.Of the invention
Gluten yield is up to 60% or more, and protein content in Gluten is up to 92% or more.
A kind of method producing Gluten proposed by the present invention, includes the following steps:
S1, wheat flour is mixed with PBS buffer solution, complex enzyme is added, be protected from light water-bath extraction, filtering obtains filtrate a；
S2, be added alkaline solution into filtrate a, carry out alkali carries, alkali extract is subjected to centrifugal treating, obtain supernatant a and
Sediment a；
S3, ethanol solution is added into sediment a, carries out alcohol extracting, alcohol extract is subjected to centrifugal treating, obtains supernatant b；
S4, supernatant a and supernatant b is mixed, adjusts pH, staticly settles, filter to obtain screening and filtrate b；By filtrate b into
Row centrifugal treating obtains sediment b；
S5, merge screening and sediment b, add water to be tuned into feed liquid, feed liquid is spray-dried, obtain Gluten.
Preferably, in S1, wheat flour is mixed with the PBS buffer solution of 0.2-0.6mol/L 1:40-60 in mass ratio, is added
Complex enzyme, is protected from light water-bath extraction, and filtering obtains filtrate a.
Preferably, in S1, complex enzyme is made of pectase and cellulase according to the mass ratio of 0.2-0.3:0.7-0.8.
Preferably, in S1, the temperature that water-bath is extracted is 45-60 DEG C, time 5-7h.
Preferably, in S2, the temperature of alkali carries is 45-65 DEG C, pH 8-10, time 2.5-5.5h.
Preferably, in S2, the rate of centrifugal treating is 1000-4000rpm, time 15-35min.
Preferably, in S3, the temperature of alcohol extracting is 50-60 DEG C, pH 7-9, time 3-6h.
Preferably, in S4, supernatant a and supernatant b is mixed, pH to 4-5 is adjusted, staticly settles 0.5-2h, filter
Screening and filtrate b；Filtrate b is subjected to centrifugal treating 10-40min, obtains sediment b.
Preferably, in S5, the mass fraction of feed liquid is 30-40wt%.
The present invention, first with compound enzymatic treatment wheat flour, is conducive to Wheat cell wall film during extracting Gluten
It is broken, the recovery rate and purity of glutelin amyloid proteins are effectively improved, and since low-temperature condition can be always maintained in operating process, it can
Ensure its bioactivity；Then alkali carries are carried out, lye can not only significantly improve the recovery rate of glutenin in Gluten, may be used also
To cut off the hydrogen bond in albumen, amido bond and disulfide bond, and some polar groups can be made to dissociate, make protein molecule surface institute band
Charge is identical, and the conjugates such as protein and starch, cellulose is promoted to separate, to increase the dissolubility of protein molecule；It connects
Carry out alcohol extracting, can effectively extract the alcohol soluble protein in wheat flour, improve the purity of Gluten；Finally in acid condition into
Row precipitating, obtains Gluten；Enzyme treated, alkali carries and alcohol extraction process combine, and the yield of Gluten is up to 60% or more, Gluten
In protein content up to 92% or more.
Specific embodiment
In the following, technical solution of the present invention is described in detail by specific embodiment.
Embodiment 1
A method of Gluten is produced, is included the following steps:
S1, wheat flour is mixed with PBS buffer solution, complex enzyme is added, be protected from light water-bath extraction, filtering obtains filtrate a；
S2, be added alkaline solution into filtrate a, carry out alkali carries, alkali extract is subjected to centrifugal treating, obtain supernatant a and
Sediment a；
S3, ethanol solution is added into sediment a, carries out alcohol extracting, alcohol extract is subjected to centrifugal treating, obtains supernatant b；
S4, supernatant a and supernatant b is mixed, adjusts pH, staticly settles, filter to obtain screening and filtrate b；By filtrate b into
Row centrifugal treating obtains sediment b；
S5, merge screening and sediment b, add water to be tuned into feed liquid, feed liquid is spray-dried, obtain Gluten.
Embodiment 2
A method of Gluten is produced, is included the following steps:
S1, wheat flour is mixed with PBS buffer solution, complex enzyme is added, be protected from light water-bath extraction, filtering obtains filtrate a；
S2, be added alkaline solution into filtrate a, carry out alkali carries, alkali extract is subjected to centrifugal treating, obtain supernatant a and
Sediment a；
S3, ethanol solution is added into sediment a, carries out alcohol extracting, alcohol extract is subjected to centrifugal treating, obtains supernatant b；
S4, supernatant a and supernatant b is mixed, adjusts pH, staticly settles, filter to obtain screening and filtrate b；By filtrate b into
Row centrifugal treating obtains sediment b；
S5, merge screening and sediment b, add water to be tuned into feed liquid, feed liquid is spray-dried, obtain Gluten；
Wherein, in S1, wheat flour is mixed with the PBS buffer solution of 0.3mol/L 1:45 in mass ratio, complex enzyme is added, keeps away
Light water-bath is extracted, and filtering obtains filtrate a.
Embodiment 3
A method of Gluten is produced, is included the following steps:
S1, wheat flour is mixed with PBS buffer solution, complex enzyme is added, be protected from light water-bath extraction, filtering obtains filtrate a；
S2, be added alkaline solution into filtrate a, carry out alkali carries, alkali extract is subjected to centrifugal treating, obtain supernatant a and
Sediment a；
S3, ethanol solution is added into sediment a, carries out alcohol extracting, alcohol extract is subjected to centrifugal treating, obtains supernatant b；
S4, supernatant a and supernatant b is mixed, adjusts pH, staticly settles, filter to obtain screening and filtrate b；By filtrate b into
Row centrifugal treating obtains sediment b；
S5, merge screening and sediment b, add water to be tuned into feed liquid, feed liquid is spray-dried, obtain Gluten；
Wherein, in S1, wheat flour is mixed with the PBS buffer solution of 0.5mol/L 1:50 in mass ratio, complex enzyme is added, keeps away
Light water-bath is extracted, and filtering obtains filtrate a；
In S1, complex enzyme is made of pectase and cellulase according to the mass ratio of 0.25:0.75；
In S1, the temperature that water-bath is extracted is 50 DEG C, time 6h；
In S2, the temperature of alkali carries is 55 DEG C, pH 9, time 3.5h；
In S2, the rate of centrifugal treating is 2000rpm, time 25min；
In S3, the temperature of alcohol extracting is 55 DEG C, pH 8, time 5h；
In S4, supernatant a and supernatant b is mixed, pH to 4.5 is adjusted, staticly settles 1h, filter to obtain screening and filtrate b；
Filtrate b is subjected to centrifugal treating 30min, obtains sediment b；
In S5, the mass fraction of feed liquid is 35wt%.
Embodiment 4
A method of Gluten is produced, is included the following steps:
S1, wheat flour is mixed with PBS buffer solution, complex enzyme is added, be protected from light water-bath extraction, filtering obtains filtrate a；
S2, be added alkaline solution into filtrate a, carry out alkali carries, alkali extract is subjected to centrifugal treating, obtain supernatant a and
Sediment a；
S3, ethanol solution is added into sediment a, carries out alcohol extracting, alcohol extract is subjected to centrifugal treating, obtains supernatant b；
S4, supernatant a and supernatant b is mixed, adjusts pH, staticly settles, filter to obtain screening and filtrate b；By filtrate b into
Row centrifugal treating obtains sediment b；
S5, merge screening and sediment b, add water to be tuned into feed liquid, feed liquid is spray-dried, obtain Gluten；
Wherein, in S1, wheat flour is mixed with the PBS buffer solution of 0.2mol/L 1:60 in mass ratio, complex enzyme is added, keeps away
Light water-bath is extracted, and filtering obtains filtrate a；
In S1, complex enzyme is made of pectase and cellulase according to the mass ratio of 0.2:0.8；
In S1, the temperature that water-bath is extracted is 45 DEG C, time 7h；
In S2, the temperature of alkali carries is 45 DEG C, pH 10, time 2.5h；
In S2, the rate of centrifugal treating is 4000rpm, time 15min；
In S3, the temperature of alcohol extracting is 60 DEG C, pH 7, time 6h；
In S4, supernatant a and supernatant b is mixed, pH to 4 is adjusted, staticly settles 2h, filter to obtain screening and filtrate b；It will
Filtrate b carries out centrifugal treating 10min, obtains sediment b；
In S5, the mass fraction of feed liquid is 40wt%.
Embodiment 5
A method of Gluten is produced, is included the following steps:
S1, wheat flour is mixed with PBS buffer solution, complex enzyme is added, be protected from light water-bath extraction, filtering obtains filtrate a；
S2, be added alkaline solution into filtrate a, carry out alkali carries, alkali extract is subjected to centrifugal treating, obtain supernatant a and
Sediment a；
S3, ethanol solution is added into sediment a, carries out alcohol extracting, alcohol extract is subjected to centrifugal treating, obtains supernatant b；
S4, supernatant a and supernatant b is mixed, adjusts pH, staticly settles, filter to obtain screening and filtrate b；By filtrate b into
Row centrifugal treating obtains sediment b；
S5, merge screening and sediment b, add water to be tuned into feed liquid, feed liquid is spray-dried, obtain Gluten；
Wherein, in S1, wheat flour is mixed with the PBS buffer solution of 0.6mol/L 1:40 in mass ratio, complex enzyme is added, keeps away
Light water-bath is extracted, and filtering obtains filtrate a；
In S1, complex enzyme is made of pectase and cellulase according to the mass ratio of 0.3:0.7；
In S1, the temperature that water-bath is extracted is 60 DEG C, time 5h；
In S2, the temperature of alkali carries is 65 DEG C, pH 8, time 5.5h；
In S2, the rate of centrifugal treating is 1000rpm, time 35min；
In S3, the temperature of alcohol extracting is 50 DEG C, pH 9, time 3h；
In S4, supernatant a and supernatant b is mixed, pH to 5 is adjusted, staticly settles 0.5h, filter to obtain screening and filtrate b；
Filtrate b is subjected to centrifugal treating 40min, obtains sediment b；
In S5, the mass fraction of feed liquid is 30wt%.
The foregoing is only a preferred embodiment of the present invention, but scope of protection of the present invention is not limited thereto,
Anyone skilled in the art in the technical scope disclosed by the present invention, according to the technique and scheme of the present invention and its
Inventive concept is subject to equivalent substitution or change, should be covered by the protection scope of the present invention.
Claims (9)
1. a kind of method for producing Gluten, which is characterized in that include the following steps:
S1, wheat flour is mixed with PBS buffer solution, complex enzyme is added, be protected from light water-bath extraction, filtering obtains filtrate a；
S2, alkaline solution is added into filtrate a, carries out alkali carries, alkali extract is subjected to centrifugal treating, obtains supernatant a and precipitating
Object a；
S3, ethanol solution is added into sediment a, carries out alcohol extracting, alcohol extract is subjected to centrifugal treating, obtains supernatant b；
S4, supernatant a and supernatant b is mixed, adjusts pH, staticly settles, filter to obtain screening and filtrate b；By filtrate b carry out from
Heart processing, obtains sediment b；
S5, merge screening and sediment b, add water to be tuned into feed liquid, feed liquid is spray-dried, obtain Gluten.
2. producing the method for Gluten according to claim 1, which is characterized in that in S1, by wheat flour and 0.2-0.6mol/
The PBS buffer solution of L 1:40-60 in mass ratio mixing, is added complex enzyme, is protected from light water-bath extraction, and filtering obtains filtrate a.
3. the method for production Gluten according to claim 1 or claim 2, which is characterized in that in S1, complex enzyme is by pectase and fibre
Plain enzyme is tieed up to form according to the mass ratio of 0.2-0.3:0.7-0.8.
4. the method for production Gluten according to claim 1 or claim 2, which is characterized in that in S1, the temperature that water-bath is extracted is
45-60 DEG C, time 5-7h.
5. the method for production Gluten according to claim 1 or claim 2, which is characterized in that in S2, the temperature of alkali carries is 45-65
DEG C, pH 8-10, time 2.5-5.5h.
6. the method for production Gluten according to claim 1 or claim 2, which is characterized in that in S2, the rate of centrifugal treating is
1000-4000rpm, time 15-35min.
7. the method for production Gluten according to claim 1 or claim 2, which is characterized in that in S3, the temperature of alcohol extracting is 50-60
DEG C, pH 7-9, time 3-6h.
8. the method for production Gluten according to claim 1 or claim 2, which is characterized in that in S4, by supernatant a and supernatant b
Mixing adjusts pH to 4-5, staticly settles 0.5-2h, filter to obtain screening and filtrate b；Filtrate b is subjected to centrifugal treating 10-
40min obtains sediment b.
9. the method for production Gluten according to claim 1 or claim 2, which is characterized in that in S5, the mass fraction of feed liquid is
30-40wt%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810761593.1A CN109007241A (en) | 2018-07-12 | 2018-07-12 | A method of producing gluten |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810761593.1A CN109007241A (en) | 2018-07-12 | 2018-07-12 | A method of producing gluten |
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| Publication Number | Publication Date |
|---|---|
| CN109007241A true CN109007241A (en) | 2018-12-18 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201810761593.1A Pending CN109007241A (en) | 2018-07-12 | 2018-07-12 | A method of producing gluten |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113527452A (en) * | 2021-06-24 | 2021-10-22 | 天津商业大学 | Method for preparing alkali-soluble glutenin by standing coagulation method |
Citations (5)
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|---|---|---|---|---|
| CN101215316A (en) * | 2008-01-09 | 2008-07-09 | 湘潭大学 | Microwave extraction method for lotus seed protein |
| CN101215318A (en) * | 2008-01-09 | 2008-07-09 | 湘潭大学 | Ultrasonic wave extraction method for lotus seed protein |
| CN102776165A (en) * | 2011-05-09 | 2012-11-14 | 白银赛诺生物科技有限公司 | Starch wheat compound enzyme and method of applying same in preparation of starch wheat and vital gluten |
| CN104365990A (en) * | 2014-11-20 | 2015-02-25 | 华中农业大学 | Wheat alcohol-soluble protein and glutelin as well as preparation method and application thereof |
| CN106418157A (en) * | 2016-10-21 | 2017-02-22 | 周荣 | Preparation method of wheat gluten flour |
-
2018
- 2018-07-12 CN CN201810761593.1A patent/CN109007241A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101215316A (en) * | 2008-01-09 | 2008-07-09 | 湘潭大学 | Microwave extraction method for lotus seed protein |
| CN101215318A (en) * | 2008-01-09 | 2008-07-09 | 湘潭大学 | Ultrasonic wave extraction method for lotus seed protein |
| CN102776165A (en) * | 2011-05-09 | 2012-11-14 | 白银赛诺生物科技有限公司 | Starch wheat compound enzyme and method of applying same in preparation of starch wheat and vital gluten |
| CN104365990A (en) * | 2014-11-20 | 2015-02-25 | 华中农业大学 | Wheat alcohol-soluble protein and glutelin as well as preparation method and application thereof |
| CN106418157A (en) * | 2016-10-21 | 2017-02-22 | 周荣 | Preparation method of wheat gluten flour |
Non-Patent Citations (1)
| Title |
|---|
| 崔春: "《食物蛋白质控制酶解技术》", 30 June 2018, 中国轻工业出版社 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113527452A (en) * | 2021-06-24 | 2021-10-22 | 天津商业大学 | Method for preparing alkali-soluble glutenin by standing coagulation method |
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