CN109006299A - A kind of radix scutellariae stock breeding and cultural method - Google Patents
A kind of radix scutellariae stock breeding and cultural method Download PDFInfo
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- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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Abstract
The invention belongs to the method and technology fields of new plant or acquisition new plant, disclose a kind of radix scutellariae stock breeding and cultural method, the radix scutellariae stock breeding and cultural method include: after radix scutellariae mature seed harvests, seed collection, 7 the first tenday period of a month of radix scutellariae fruiting period~late August are first placed in ventilation and dry in the air 1~2 day after harvesting, continue to cure to seed, then it dries, threshing is selected；Radix scutellariae cultivates selection of land site preparation, sowing, field management, extracts bud, the prevention and control of plant diseases, pest control；Pick and process harvests when second year aerial part in October is withered after cultivation.Annual root 6~7g of single plant counterpoise, 16~17g of biennial single plant counterpoise.Content of baicalin is high in root, is all larger than 10%.Its cultivation adaptability is stronger, it is disease-resistant, in terms of it is more excellent.This standard is suitable for the production of Shandong District " Shandong a kind of reed mentioned in ancient books 1 " radix scutellariae.
Description
Technical field
The invention belongs to new plant or obtain new plant method and technology field more particularly to a kind of radix scutellariae stock breeding with
Cultural method.
Background technique
Currently, the prior art commonly used in the trade is such that radix scutellariae is parts of generic medicinal plants, due to increasingly subtracting for wild resource
Less and the continuous expansion of usage amount, current medicinal radix scutellariae is based on cultivation product, and cultivated area constantly expands.Radix scutellariae
(Scutellaria baicalensis Georgi) be Lamiaceae plant, be used as medicine with root, have heat-clearing and damp-drying drug, purging fire for removing toxin,
The effect of hemostasis, miscarriage prevention.Radix scutellariae is a dispersed species, spans multiple climate zones and soil band.Although in its plant of different regions
The features such as plant height is short, branch is how many, color, the blade young pilose antler of flower have some variations, but in general, blade and flowers and fruits form
Than more consistent, the record of infraspecies taxonomy unit there are no.But investigation discovery different sources radix scutellariae has some differences on mode of appearance
It is different, as stem have it is green, two kinds purple, Hua Youzi, powder, white point, and plant height, branch amount, overground part fresh weight, the thickness of root are long
It is short, whether there is or not the biological properties such as the withered heart larger difference, the phenological period is also not quite similar, and shows there is hereditary difference in radix scutellariae kind.
In addition to this, the radix scutellariae cultivation adaptability of different germplasm is different, and the ability for fighting extraneous poor environment is variant, it is often more important that
The content of principle active component scutelloside etc. has larger difference." Shandong a kind of reed mentioned in ancient books 1 " radix scutellariae (Scutellaria baicalensis
It Georgi is) perennial herb, plant height 45-50cm, stem is more sturdy, and stem is more apparent, and stem thickness is strong, and stem 2-3 item has more thereon
Number side shoot, green, coat.Leaf surface bottle green, the food value of leaf is thicker, and blade is larger, long 3-4.2cm, wide 0.6-0.9cm, coat.Leaf
Staple length at edge and lower surface vein and it is close, be in eyelashes.Rachis is longer, and thick, and long 15-20cm, flower arrangement is sparse, and flower is about
1.8cm, purple to bluish violet, base portion band yellow, coat.Fruit black, larger, long 1.2-2.6mm, wide 1.5-1.9mm, thousand
Weight 1.9-2.1g.Root is coarseer, annual root single plant counterpoise 6-7g, biennial single plant counterpoise 16-17g.Content of baicalin in root
Height is all larger than 10%.Its cultivation adaptability is stronger, it is disease-resistant, in terms of it is more excellent.
In conclusion problem of the existing technology is:
(1) it is produced without breeding for radix scutellariae, it is serious that radix scutellariae germplasm mixes phenomenon.
(2) radix scutellariae medicinal materials unstable quality.
(3) cultivation adaptability is weaker, in disease-resistant, degeneration-resistant deficiency.
Solve the difficulty and meaning of above-mentioned technical problem:
Radix scutellariae is a dispersed species, spans multiple climate zones and soil band.Although in different regions its plant height, point
The features such as branch is how many, color, blade young pilose antler of flower have some variations, but in general, blade and flowers and fruits form than more consistent,
It there are no the record of infraspecies taxonomy unit.But investigation discovery different sources radix scutellariae has some differences on mode of appearance, as stem has
It is green, two kinds purple, Hua Youzi, powder, white point, and plant height, branch amount, the biological properties such as overground part fresh weight have larger area
Not, the phenological period is also not quite similar, and shows there is hereditary difference in radix scutellariae kind.
Currently, being continuously increased due to radix scutellariae survival dose, wild resource is destroyed seriously, and medicinal radix scutellariae is based on cultivation product.
Although artificial cultivation has achieved some achievements, there is presently no cultivate kind or Farm Races best in quality.Some
Radix scutellariae forms some variations after several generations is cultivated, such as root thickness length, whether there is or not the withered hearts difference, principle active component
The content of scutelloside is also variant.Quality is irregular between germplasm, and there are also quality deterioration phenomenons.
Further, since there is blindness when medicinal herb grower introduces a fine variety again merely through introducing a fine variety and taming in short term in most of cultivation radix scutellariae, from
And cultivated population germplasm is caused to mix, very different between plant, commercial quality is unstable.Clinical efficacy is seriously affected.
In view of the foregoing, it is extremely urgent to carry out the work of radix scutellariae Variety Selection.The difficulty of Chinese medicine fine-variety breeding is much big
In general crops, main cause be requirement other than the high yield of agricultural product breeding to be had, resistance, for drug effect also compared with
It is high, it is desirable that Chinese medicine breeding active constituent content is high, good drug efficacy.It therefore must be in the investigation to domestic Germplasm Resources of Scutellaria Baicalensis Georgi, receipts
Collection on the basis of arranging, introduce and cultivating, by the variety comparative test of system, filters out germplasm best in quality, and utilize modern
The methods of molecular biology detect population between, the hereditary difference in population, for further carry out radix scutellariae breeding industrialization production,
The output and quality of radix scutellariae is greatly improved, lays a solid foundation.
Summary of the invention
In view of the problems of the existing technology, the present invention provides a kind of radix scutellariae stock breeding and cultural methods.
The invention is realized in this way this kind of radix scutellariae stock breeding and cultural method radix scutellariae, the radix scutellariae stock breeding with
Cultural method includes: after radix scutellariae mature seed harvests, and seed collection in 7 the first tenday period of a month of radix scutellariae fruiting period~late August, is first placed in after harvesting
Ventilation is dried in the air 1~2 day, is continued to cure to seed, then be dried, and threshing is selected；
Radix scutellariae cultivates selection of land site preparation, sowing, field management, extracts bud, the prevention and control of plant diseases, pest control；Pick and process, after cultivation
Second year aerial part in October harvests when withered.
Further, the radix scutellariae stock breeding method includes:
Step 1: kind is chosen；
Step 2: seedling field environmental selection；
Step 3: radix scutellariae 45~50cm of plant height has most side shoots on stem 2~3, green, coat；
Step 4: after the harvesting of radix scutellariae mature seed, the seed of selected bright in color, full seed, 95% or more purity, thousand
Grain weight 1.93g or more, 80% or more germination percentage, dry free from admixture；
Step 5: seedling field management: selection of land site preparation, seed seedling-raising, seed field management, the prevention and control of plant diseases, pest control；
Step 6: seed collection, 7 the first tenday period of a month of radix scutellariae fruiting period~late August, fruit are harvested when being in light brown, are first set after harvesting
It dries in the air 1~2 day in ventilation, continues to cure to seed, then dry, threshing is selected；
Step 7: packaging storage, seed fill cloth bag and save.
Further, the seed field management includes: seedling management, tillage and weeding, top dressing, irrigation.
Further, the prevention and control of plant diseases, pest control include: control principle, root rot prevention and treatment, leaf blight prevention and treatment, controlling powdery mildew,
Root knot nematode control.
Further, the radix scutellariae breeding cultural method includes:
Step 1: kind is chosen；
Step 2: seedling field environment；
Step 3: radix scutellariae is perennial herb, and 45~50cm of plant height, stem is more sturdy, and stem is more apparent, and stem thickness is strong, stem 2
~3, there are most side shoots thereon, green, coat；
Step 4: selection of land site preparation, field management, extracts bud, the prevention and control of plant diseases, pest control at sowing；
Step 5: pick and process harvests when second year aerial part in October is withered after cultivation, to deep-cut, take full root, keep away
Exempt to hurt root and be completely cured, removes soil and residual stem, dry rapidly or dry；
Step 6: packaging storage, packaging material is using dry, cleaning, free from extraneous odour and does not influence the material of quality and is made.
Further, the sowing includes: live streaming, culturing and transplanting seedlings.
Further, the seedling field management: seedling management, tillage and weeding, top dressing, irrigation.
Further, the prevention and control of plant diseases, pest control include: control principle, root rot prevention and treatment, leaf blight prevention and treatment, controlling powdery mildew,
Root knot nematode control.
In conclusion advantages of the present invention and good effect are as follows: " Shandong a kind of reed mentioned in ancient books 1 " root is coarseer, and single or middle-lower part is
Two, surface dark-brown, hole skin is lateral, fragmentary to be distributed.Vertical wrinkle is fine and closely woven.Long 23~29cm, 1.5~2cm of diameter, section are dark brown
Yellow, area of phloem is larger, accounts for about 1/2.Bast is more close with xylem color.Fibroid is stronger.Matter is crisp, frangibility,
Section foresythia, it is uneven, it is slightly in en plaque." Shandong a kind of reed mentioned in ancient books 1 " effective component content of baicalin is high, and 2013,2014 through Jinan
The detection of medicine inspecting institute of city, scutelloside average content are 17.1% (life in 2 years) and 11.6% (life in 1 year), are increased respectively than control
22.7% and 8.1%.
In addition, the present invention has been achieved for the drug inspection report at Jinan City food and medicine analysis center, reference number of a document is
JLO3-5.10-2012。
Detailed description of the invention
Fig. 1 is radix scutellariae stock breeding method flow diagram provided in an embodiment of the present invention.
Fig. 2 is radix scutellariae breeding cultural method flow chart provided in an embodiment of the present invention.
Fig. 3 is the RAPD identification flow figure of different germplasm of baicalin provided in an embodiment of the present invention.
Specific embodiment
In order to make the objectives, technical solutions, and advantages of the present invention clearer, with reference to embodiments, to the present invention
It is further elaborated.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, it is not used to
Limit the present invention.
Application principle of the invention is explained in detail with reference to the accompanying drawing.
Field not continuous cropping continuous cropping for seed production.Seed-breeding field contiguous plant, the distance between other crops will foots
It is enough big.Cultivation field wants fertile, simple grain thin planting, improves breeding coefficient.That pulls out Tanaka in time mixes strain or mutant strain.Original seed is 3
The end of month sowing, plant left to run to seed should be 2~3 years plant.The early July of next year to late August adopts in batches when the thin out brown of pericarp
It receives, lays seed, dry in ventilating and cooling place, storage is in a cool and dry place
As shown in Figure 1, radix scutellariae stock breeding method provided in an embodiment of the present invention includes:
S101: selection kind, Shandong a kind of reed mentioned in ancient books 1；
S102: seedling field environment, " Shandong a kind of reed mentioned in ancient books 1 " radix scutellariae nursery environmental condition should meet water quality, air quality, soil quality
National standard；
S103: varietal characteristic, " Shandong a kind of reed mentioned in ancient books 1 " radix scutellariae be perennial herb, 45~50cm of plant height, stem is more sturdy, stem compared with
Obviously, stem thickness is strong, stem 2~3, there is most side shoots thereon, green, coat；
S104: corresponding seed quality criteria, after the harvesting of " Shandong a kind of reed mentioned in ancient books 1 " radix scutellariae mature seed, selected bright in color, full seed
Seed, 95% or more purity, mass of 1000 kernel 1.93g or more, 80% or more germination percentage, dry free from admixture；
S105: seedling field management: selection of land site preparation, seed seedling-raising, seed field management, the prevention and control of plant diseases, pest control；
S106: seed collection, 7 the first tenday period of a month of radix scutellariae fruiting period~late August, fruit are harvested when being in light brown, are first placed in after harvesting
Ventilation is dried in the air 1~2 day, is continued to cure to seed, then be dried, and threshing is selected；
S107: packaging storage, seed fill cloth bag and save.
" Shandong a kind of reed mentioned in ancient books 1 " radix scutellariae nursery environmental condition provided in an embodiment of the present invention should meet following provisions: water quality reaches
5084 standard above grade 2 of agricultural irrigation water quality standard GB；Atmospheric environment reach 3095 second level of ambient air quality GB with
Upper standard；Soil reaches 15618 standard above grade 2 of standard of soil environment quality GB；
Seed field management provided in an embodiment of the present invention includes: seedling management, tillage and weeding, top dressing, irrigation；
The prevention and control of plant diseases, pest control provided in an embodiment of the present invention includes: control principle, root rot prevention and treatment, leaf blight prevention and treatment, white powder
Disease prevention and treatment, root knot nematode control.
" Shandong a kind of reed mentioned in ancient books 1 " preferably uses seminal propagation, live streaming or culturing and transplanting seedlings.Live streaming in late March, by line-spacing 30~
35cm opens the shallow ridges live streaming of 1cm on ridge.Seed is handled through vernalization before broadcasting, per acre 0.5~0.8kg of sowing quantity, covers mulch,
Go out full stand within 10~15 days.September part sowing after seed harvest of culturing and transplanting seedlings, next year late March to early April are transplanted, are pressed
Spacing in the rows 12cm is colonized in crop field, and 10000 plants or so per acre of planting density.During emergence extremely envelope ridge, weeding 3~4 times.In conjunction with intertillage
Timely thinning, is filled the gaps with seedlings at final singling.Growth period applies N, P, K compound fertilizer 1~2 time every year.Rainy season drains in time.Second year October after cultivation
Last ten-days period harvesting, deep-cuts, takes full root, remove soil and residual stem, dry rapidly or dry.
As shown in Fig. 2, radix scutellariae breeding cultural method provided in an embodiment of the present invention includes:
S201: selection kind, Shandong a kind of reed mentioned in ancient books 1；
S202: seedling field environment, " Shandong a kind of reed mentioned in ancient books 1 " radix scutellariae nursery environmental condition should meet water quality, air quality, soil quality
National standard；
S203: varietal characteristic, " Shandong a kind of reed mentioned in ancient books 1 " radix scutellariae be perennial herb, 45~50cm of plant height, stem is more sturdy, stem compared with
Obviously, stem thickness is strong, stem 2~3, there is most side shoots thereon, green, coat；
S204: cultivation technique: selection of land site preparation, field management, extracts bud, the prevention and control of plant diseases, pest control at sowing；
S205: pick and process harvests when second year aerial part in October is withered after cultivation, to deep-cut, take full root, avoid
Hurt root and be completely cured, removes soil and residual stem, dry rapidly or dry；
S206: packaging storage, packaging material is using dry, cleaning, free from extraneous odour and does not influence the material of quality and is made.
" Shandong a kind of reed mentioned in ancient books 1 " radix scutellariae nursery environmental condition provided in an embodiment of the present invention should meet following provisions: water quality reaches
5084 standard above grade 2 of agricultural irrigation water quality standard GB；Atmospheric environment reach 3095 second level of ambient air quality GB with
Upper standard；Soil reaches 15618 standard above grade 2 of standard of soil environment quality GB；
Sowing provided in an embodiment of the present invention includes: live streaming, culturing and transplanting seedlings；
Seedling field management provided in an embodiment of the present invention: seedling management, tillage and weeding, top dressing, irrigation；
The prevention and control of plant diseases, pest control provided in an embodiment of the present invention includes: control principle, root rot prevention and treatment, leaf blight prevention and treatment, white powder
Disease prevention and treatment, root knot nematode control.
The RAPD of difference germplasm of baicalin provided by the invention, which identifies, includes:
S301 acquires the radix scutellariae tender leaf of different germplasm, extracts the total chromosome of radix scutellariae: winning tender group of the children of different germplasm of baicalin
Knit 0.3g, be ground into powder in liquid nitrogen, be added be preheated to 65 DEG C DNA Extraction buffer (100mmol L-1Tris ×
HCl, 1.4mol L-1NaCl, 20mmol L-1EDTA, 2% (w/v) CTAB, 2% (v/v) 2 mercapto ethanol 0.4%), it mixes,
65 DEG C of warm bath 15min；It puts to room temperature, isometric chloroform: isoamyl alcohol (24:1) is added, mix, place 10min；4 DEG C,
12000rpm is centrifuged 15min, recycles supernatant, and the CTAB/NaCl solution (10% that 1/10 volume is pre-heated to 65 DEG C is added
CTAB, 0.7MNaCl), it is mixed by inversion.Isometric chloroform: isoamyl alcohol (24:1) extracting mixes, 4 DEG C, 12000rpm centrifugation
15min recycles supernatant.Be added isometric CTAB precipitated liquid (1% (w/v) CTAB, 50mmol L-1Tris × HCl,
10mmol L-1EDTA, pH8.0), 4 DEG C, 3000rpm is centrifuged 5min, supernatant is abandoned, with TE buffer (10mmol L- with high salt
1Tris × HCl, 1mol L-1NaCl, 0.1mmol L-1EDTA), the molten precipitating of weight.2 times of volume dehydrated alcohol precipitating cores are added
Acid mixes well, and -20 DEG C of placement 30min, 4 DEG C, 12000rpm is centrifuged 15min.Abandon supernatant, 70% ethanol washing precipitating, room temperature
Dry 20min, with containing whether there is or not the molten DNA of the TE of the Pancreatic RNase of DNA enzymatic (20ml/ml) (pH8.0) weight, 4 DEG C of storages.
S302, ultraviolet spectrophotometry detect radix scutellariae DNA sample DNA integrality: ultraviolet spectrophotometry detects DNA sample
Concentration, 0.7% agarose gel electrophoresis detect DNA integrality；
S303 is expanded using suitable RAPD program, and each reaction is repeated 3 times: using 25l reaction system, include:
DdH2O:19.4 μ l, 10 × EasyTaq DNA Polymerase Buffer:2.5 μ l, template: 0.3 μ l, dNTPs:2 μ l draws
Object: 0.3 μ l, EasyTaq DNA Polymerase:0.5 μ l.Reaction condition are as follows: 95 DEG C of initial denaturation 10min, then carry out 40 and follow
Ring: 95 DEG C of denaturation 1min, 36 DEG C of annealing 1min, 72 DEG C of extension 90s.After 35 circulations, 72 DEG C of extension 10min.Each reaction is extremely
It is repeated 3 times less；
S304, electrophoresis are simultaneously taken a picture, and different germplasm of baicalin DNA results are analyzed: using agarose gel electrophoresis: electrophoretic buffer
For Tris- acetate buffer solution (TAE): 40mmol L-1Tris-HCL, 2mmol L-1EDTA, 20mmol L-1 sodium acetate, pH
8.0；1% (w/v) Ago-Gel, L-1EB containing 0.5mg, 85V electrophoresis 1.5h.It is molten that 0.5 μ g ml-1EB is dipped in after electrophoresis
5~10min is dyed in liquid, is observed and is taken a picture under gel imager.
Since the radix scutellariae of different germplasm is more similar on mode of appearance, conventional discrimination method is yellow to different germplasm of the same race
Accurate identify of a kind of reed mentioned in ancient books is difficult to gather effect.Currently, increasingly developed and perfect with biotechnology, new analysis and detection technique is a large amount of
It emerges in large numbers, the biotechnologys such as RAPD, AFLP, SST of based on PCR technology are applied to Germplasm Identification, affiliation point more and more
The various fields such as analysis.Wherein RAPD is a kind of effective molecule labelling method, is taken in terms of the close germplasm analysis of affiliation
Obtained good result.The present invention analyzes the radix scutellariae of different germplasm using RAPD technology, mentions for the identification of radix scutellariae germplasm
For foundation.
Invention is further explained combined with specific embodiments below；
Embodiment 1；
In regional testings in 2012, raw medicinal herbs (dry product) per mu yield in 2 years is 592 kilograms average, increases than control Juxian County farm variety
Produce 13.8%；Pilot production in 2013, raw medicinal herbs (dry product) per mu yield in 1 year is 340 kilograms average, than control Juxian County farm variety volume increase
22%.
" Shandong a kind of reed mentioned in ancient books 1 " cultivation adaptability is stronger, it is disease-resistant, in terms of it is more excellent.This standard is suitable for Shandong
The production of area " Shandong a kind of reed mentioned in ancient books 1 " radix scutellariae.
Try " Shandong a kind of reed mentioned in ancient books 1 " output and quality application form in 1 Mengyin, Shandong area of table
2 root yield of table and quality determination
Mengyin Result of the plot trial shows that a kind of reed mentioned in ancient books No. 1 growth and development stage in Shandong blooms obviously earlier than check variety and seed maturation shifts to an earlier date
9~10 days.For root yield than check variety volume increase 9.7%, content of baicalin is higher than control 30.7%.
Try " Shandong a kind of reed mentioned in ancient books 1 " output and quality application form in 3 Taian Shandong area of table
4 root yield of table and quality determination
Tai'an base Result of the plot trial shows that a kind of reed mentioned in ancient books No. 1 growth and development stage in Shandong shifts to an earlier date 8 days than check variety, yield increased product
Kind volume increase 16.0%, content of baicalin is higher than control 25.9%.
Pass through the experiment in cultivation of the 3 area's pilots in Shandong, it was demonstrated that " Shandong a kind of reed mentioned in ancient books 1 " is in plant resistance to environment stress, root yield and quality
It is superior to check variety, the results are shown in Table 5,6.
Table 5 " Shandong a kind of reed mentioned in ancient books 1 " area's reagent material (life in 2 years) yield
Table 6 " Shandong a kind of reed mentioned in ancient books 1 " area's reagent material (life in 2 years) principle active component content of baicalin
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all in essence of the invention
Made any modifications, equivalent replacements, and improvements etc., should all be included in the protection scope of the present invention within mind and principle.
Claims (8)
1. a kind of radix scutellariae stock breeding and cultural method, which is characterized in that the radix scutellariae stock breeding and cultural method include: Huang
After the harvesting of a kind of reed mentioned in ancient books mature seed, seed collection, 7 the first tenday period of a month of radix scutellariae fruiting period~late August are first placed in ventilation and dry in the air 1~2 day after harvesting,
Continue to cure to seed, then dry, threshing is selected；
Radix scutellariae cultivates selection of land site preparation, sowing, field management, extracts bud, the prevention and control of plant diseases, pest control；Pick and process, second after cultivation
Year aerial part in October harvests when withered.
2. radix scutellariae stock breeding as described in claim 1 and cultural method, which is characterized in that the radix scutellariae stock breeding method
Include:
Step 1: kind is chosen；
Step 2: seedling field environmental selection；
Step 3: radix scutellariae 45~50cm of plant height has most side shoots on stem 2~3, green, coat；
Step 4: after the harvesting of radix scutellariae mature seed, the seed of selected bright in color, full seed, 95% or more purity, mass of 1000 kernel
1.93g or more, 80% or more germination percentage, dry free from admixture；
Step 5: seedling field management: selection of land site preparation, seed seedling-raising, seed field management, the prevention and control of plant diseases, pest control；
Step 6: seed collection, 7 the first tenday period of a month of radix scutellariae fruiting period~late August, fruit are harvested when being in light brown, are first placed in after harvesting logical
It dries in the air at wind 1~2 day, continues to cure to seed, then dry, threshing is selected；
Step 7: packaging storage, seed fill cloth bag and save.
3. a kind of reed mentioned in ancient books No. 1 radix scutellariae stock breeding in Shandong as claimed in claim 2 and cultural method, which is characterized in that the seed field pipe
Reason includes: seedling management, tillage and weeding, top dressing, irrigation.
4. a kind of reed mentioned in ancient books No. 1 radix scutellariae stock breeding in Shandong as claimed in claim 2 and cultural method, which is characterized in that the pest and disease damage is anti-
Control includes: control principle, root rot prevention and treatment, leaf blight prevention and treatment, controlling powdery mildew, root knot nematode control.
5. radix scutellariae stock breeding as described in claim 1 and cultural method, which is characterized in that the radix scutellariae breeding cultural method
Include:
Step 1: kind is chosen；
Step 2: seedling field environment；
Step 3: radix scutellariae is perennial herb, and 45~50cm of plant height, stem is more sturdy, and stem is more apparent, and stem thickness is strong, stem 2~3
Item has most side shoots thereon, green, coat；
Step 4: selection of land site preparation, field management, extracts bud, the prevention and control of plant diseases, pest control at sowing；
Step 5: pick and process harvests when second year aerial part in October is withered after cultivation, to deep-cut, take full root, avoid hurting
It root and is completely cured, removes soil and residual stem, dry rapidly or dry；
Step 6: packaging storage, packaging material is using dry, cleaning, free from extraneous odour and does not influence the material of quality and is made.
6. radix scutellariae stock breeding as claimed in claim 5 and cultural method, which is characterized in that the sowing includes: live streaming, educates
Transplantation of seedlings.
7. a kind of reed mentioned in ancient books No. 1 radix scutellariae stock breeding in Shandong as claimed in claim 5 and cultural method, which is characterized in that the seedling field management:
Seedling management, tillage and weeding, top dressing, irrigation.
8. a kind of reed mentioned in ancient books No. 1 radix scutellariae stock breeding in Shandong as claimed in claim 5 and cultural method, which is characterized in that the pest and disease damage is anti-
Control includes: control principle, root rot prevention and treatment, leaf blight prevention and treatment, controlling powdery mildew, root knot nematode control.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN118064637A (en) * | 2024-04-17 | 2024-05-24 | 山东省中医药研究院 | SNP molecular marker for identifying baihua baicalensis, kit and application of SNP molecular marker |
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| CN113796213A (en) * | 2020-06-15 | 2021-12-17 | 南京中医药大学 | Method for high-quality recovery and utilization of overground and underground parts of scutellaria baicalensis |
| CN118064637A (en) * | 2024-04-17 | 2024-05-24 | 山东省中医药研究院 | SNP molecular marker for identifying baihua baicalensis, kit and application of SNP molecular marker |
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