CN108992375B - Anticorrosive composite for cosmetics and preparation method thereof, purposes and cosmetics - Google Patents
Anticorrosive composite for cosmetics and preparation method thereof, purposes and cosmetics Download PDFInfo
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- CN108992375B CN108992375B CN201810893254.9A CN201810893254A CN108992375B CN 108992375 B CN108992375 B CN 108992375B CN 201810893254 A CN201810893254 A CN 201810893254A CN 108992375 B CN108992375 B CN 108992375B
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/10—General cosmetic use
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/52—Stabilizers
- A61K2800/524—Preservatives
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Abstract
The present invention provides a kind of anticorrosive composite for cosmetics and preparation method thereof, purposes and cosmetics, are related to cosmetic technical field.Radix Sangusorbae extract, Tianshan Mountains violet extract are combined by the anticorrosive composite, as the active constituent in anticorrosive composite, the composition of acquisition has good antibacterial action, and all there is good bacteriostasis to common microbiological in cosmetics, such as Candida albicans, aspergillus niger and E.coli and yeast.It include cosmetics tools the advantages of being not easy putrid and deteriorated and lower sensitization of above-mentioned anticorrosive composite.
Description
Technical field
The present invention relates to cosmetic technical fields, are especially for anticorrosive composite and its preparation side of cosmetics
Method, purposes and cosmetics.
Background technique
Preservative is microorganism can be prevented to grow, a kind of additive for preventing product rotten.In cosmetics, addition is anti-
Rotten agent can be rotten, spoiled, corrupt to avoid product caused by microbial activities in cosmetics, extends the shelf life of product, prevents
Only consumer is because generate adverse effect using the cosmetics of microbial contamination.Currently, China's " cosmetics safety technology rule
Model " (2015 editions) regulation mutatis mutandis preservative 51, wherein widely used preservative mainly has parabens, Form aldehyde release body
Class, benzyl alcohol and its derivative, methylisothiazolinone or Phenoxyethanol etc., it is many kinds of.
Most of cosmetics preservatives are all that cost is relatively low, based on the chemical synthesis substance that is readily produced, although it can be with
Effectively keep the stabilization of cosmetics, but with the development of science and technology, many cosmetics preservatives be all proved to have it is certain negative
Effect, for example Form aldehyde release body class preservative is there are certain carcinogenic risk, methylisothiazolinone have certain irritation and
Sensitization can cause serious dermatitis, while the preservatives such as Phenoxyethanol, parabens are also standby because of its potential safety
By the query and worry of numerous scholars and consumer.Therefore, safe, mild, efficient protective system is developed from natural plants
It is the research hotspot of current cosmetics corrosion-resistant field as the substitute of chemical preservative.
The preservative of natural botanical source be effective bacteriostatic ingredients to be extracted in natural plants as chief active at
Point, currently, natural plant preservative on the market is actually rare, because it still has many deficiencies, for example color is deep, taste
Greatly, the problems such as reducing cosmetics series viscosity and influencing stability.
In view of this, the present invention is specifically proposed.
Summary of the invention
The first object of the present invention is to provide a kind of anticorrosive composite for cosmetics, and the anticorrosive composite is with natural
Active constituent of the ingredient as antibacterial has excellent anti-corrosion effect.
The second object of the present invention is to provide a kind of preparation side of above-mentioned anticorrosive composite, and this method has simple process
With easy to operate advantage.
The third object of the present invention is to provide a kind of above-mentioned anticorrosive composite and is preparing the purposes in cosmetics.
The fourth object of the present invention is to provide a kind of cosmetics comprising above-mentioned anticorrosive composite.
In order to solve the above technical problems, spy of the present invention adopts the following technical scheme that
A kind of anticorrosive composite for cosmetics, the active raw materials of the anticorrosive composite include Radix Sangusorbae extract
With Tianshan Mountains violet extract；
Preferably, the Radix Sangusorbae extract includes long fringe Radix Sangusorbae extract.
Preferably, the content of saponin(e is not less than the 80% of Radix Sangusorbae extract gross mass in the Radix Sangusorbae extract.
Preferably, the content of general flavone is not less than Tianshan Mountains violet extract gross mass in the Tianshan Mountains violet extract
85%.
Preferably, the raw material of the anticorrosive composite further includes solvent；
Preferably, the solvent includes alcohols, lipid, ethers or water, and the solvent is preferably water, more preferably go from
Sub- water.
Preferably, the raw material of the anticorrosive composite further includes auxiliary material, and the auxiliary material includes moisturizer and/or solubilizer；
Preferably, the moisturizer includes polyalcohol；
Preferably, the polyalcohol includes Sensiva SC50, pungent glycol, 1,2- pentanediol or 1, and one in 2- hexylene glycol
Kind is a variety of；
Preferably, the solubilizer is non-ionic solubilizer, and the moisturizer includes Sensiva SC50 and pungent glycol,
Or 1,2- pentanediol and 1,2- hexylene glycol；
Preferably, the solubilizer includes Cremophor RH40, PEG-60 rilanit special or polyvinylpyrrolidine
One of ketone is a variety of；
Preferably, the solubilizer includes Cremophor RH40.
Preferably, according to mass percent meter, each raw material dosage is as follows: Radix Sangusorbae extract 0.5%-10%, Tianshan Mountains violet
Extract 1.5%-15%, solubilizer 5%-25% and moisturizer 10%-60%, surplus are water；
It is highly preferred that each raw material dosage is as follows according to mass percent meter: Radix Sangusorbae extract 1.5%-8%, Tianshan Mountains violet
Extract 3%-12%, solubilizer 8%-20% and moisturizer 25%-50%, surplus are water；
It is further preferred that each raw material dosage is as follows according to mass percent meter: Radix Sangusorbae extract 2.5%-7.5%, day
Mountain violet extract 3.5%-10.5%, solubilizer 10%-20% and moisturizer 30%-45%, surplus are water.
The present invention also provides a kind of preparation method of above-mentioned anticorrosive composite, which includes: to extract garden burnet
Object, Tianshan Mountains violet extract and optionally auxiliary material mixing, obtain the anticorrosive composite.
Preferably, the preparation method include: first by Radix Sangusorbae extract and Tianshan Mountains violet extract be dissolved in deionized water with
In the mixture of moisturizer, adds solubilizer and be uniformly mixed, obtain the anticorrosive composite after isolating insoluble matter.
The present invention also provides a kind of above-mentioned anticorrosive composites to prepare the purposes in cosmetics.
The present invention also provides a kind of cosmetics comprising above-mentioned anticorrosive composite, the cosmetics include skin care cream,
Moisturizing lotion, moisture retention water, facial treatment essence, mildy wash or facial mask.
Compared with prior art, the invention has the following beneficial effects:
Provided by the present invention for the anticorrosive composite of cosmetics, Radix Sangusorbae extract, Tianshan Mountains violet extract are subjected to group
Cooperation is the active constituent in anticorrosive composite, and the composition of acquisition has good broad-spectrum antibacterial action, and in cosmetics
Common microbiological, such as Candida albicans, aspergillus niger and Escherichia coli, saccharomycete all have good bacteriostasis.
The preparation side of above-mentioned anticorrosive composite provided by the invention has the advantages that simple process and easy to operate.The present invention
The above-mentioned anticorrosive composite provided is preparing the purposes in cosmetics, has the advantages that widely used, can be applied to multiple functions
In the cosmetic product of diversified forms.And the cosmetics provided by the invention comprising above-mentioned anticorrosive composite have both not perishable
The advantages of losing rotten and lower sensitization.
Specific embodiment
Technical solution of the present invention is clearly and completely described below in conjunction with embodiment, it is clear that described reality
Applying example is a part of the embodiment of the present invention, instead of all the embodiments.Based on the embodiments of the present invention, the common skill in this field
Art personnel every other embodiment obtained without making creative work belongs to the model that the present invention protects
It encloses.The person that is not specified actual conditions in embodiment, carries out according to conventional conditions or manufacturer's recommended conditions.Agents useful for same or instrument
Production firm person is not specified, is the conventional products that can be obtained by commercially available purchase.
The present invention provides a kind of anticorrosive composite for cosmetics, the active constituent of the anticorrosive composite includes ground
Elm extract and Tianshan Mountains violet extract.Radix Sangusorbae extract, Tianshan Mountains violet extract are combined by the present invention, the combination of acquisition
Object has good broad-spectrum antibacterial action, and to common microbiological in cosmetics, such as Candida albicans, aspergillus niger and large intestine bar
Bacterium and female bacterium etc. all have good bacteriostasis.
Garden burnet (Sanguisorba officinalis L.) is rosaceae (R.osaceae) burnet (Sanguisorba
Linn.) plant.Perennial herb, high 30-120cm, mesad are a kind of common Chinese medicine.It is recorded according to Chinese Pharmacopoeia,
It is garden burnet bitter, acid, puckery, it is slightly cold, return liver, large intestine channel；For hematochezia, hemorrhoid blood, bloody flux, metrorrhagia and metrostaxis, scald, carbuncle sore tumefacting virus.
Nutriment and physiologically active ingredient rich in Radix Sangusorbae extract, for example, flavones ingredient, saponins at
Point, the other compositions such as tannin constituents, steroidal and its methods of glycosides, carbohydrate content.Saponin component rich content in garden burnet, contains
There are a large amount of Triterpene saponins, such as garden burnet glycosides, sanguisorbin, 28 β-D- glucopyranoside of slope film alkyd and bee saage alkyd glucosides
Deng having good hemostasis, the effect of antitumor, antiallergy, anti-inflammation detumescence ground.Garden burnet is to typhoid bacillus, meningococcus, Fu Shi
Shigella dysenteriae etc. is inhibited.
Garden burnet has multiple kinds and mutation in China, such as: garden burnet (Sanguisorba officinalis L.) comes into leaves ground
Elm (S.longifolia BertL), long fringe garden burnet (S.grandifoloa (maxim.) makino), Big White Flower garden burnet
(Sanguisorba sitchensis C.A.Mey), spire garden burnet (S.tenuifolia Fisch.ex link.), edelweiss
Garden burnet (S.parriflora (Maxim.) Takeda), pink blossom garden burnet (S.officinalis L.var.carnea (Fisch)
Regal ex Maxim).Due to containing richer active material in long fringe garden burnet, present invention preferably uses long fringe garden burnets
Extract as the active constituent in anticorrosive composite.Preferably, the content for improving saponin(e in Radix Sangusorbae extract, can be improved
The anti-corrosion effect of anticorrosive composite, the anti-corrosion when content of saponin(e is not less than the 80% of Radix Sangusorbae extract gross mass in Radix Sangusorbae extract
Better effect.Since saponin content is abundant in garden burnet root, saponin extract is preferably prepared as raw material using the root of garden burnet.
Tianshan Mountains violet (Viola tianschanica Maxim) is Violaceae violet platymiscium, with clearing heat and detoxicating
Effect, be mainly used for it is various generate heat, catch cold, catching a cold, pleurisy, pneumonia, pharyngitis, furunculosis swell the diseases such as carbuncle.Contain in the violet of Tianshan Mountains
Volatile oil, chromocor compound, alkaloids and microelement abundant.Wherein general flavone is main in Tianshan Mountains violet extract
Antimicrobial component.
Flavones is a kind of natural products with the basic parent nucleus of C6-C3-C6, and flavones can be divided into flavonoids, flavonols, different
Flavonoids, chalcone, anthocyan.Flavone compound has good anti-oxidant and antibacterial action.Preferably, day is improved
The anti-corrosion effect of anticorrosive composite, general flavone in the violet extract of Tianshan Mountains can be improved in the content of saponin(e in the violet extract of mountain
Content be not less than Tianshan Mountains violet extract gross mass 85% when anti-corrosion effect more preferably.
In some alternative embodiments, the anticorrosive composite further includes solvent, and optionally, the solvent for example may be used
Think but be not limited to alcohols, lipid, ethers or water, the solvent is preferably water, more preferably deionized water.
In some alternative embodiments, the anticorrosive composite further includes auxiliary material, and the auxiliary material includes moisturizer, increases
Solvent, fumet, stabilizer or emulsifier etc., it is to be understood that the acceptable customary adjuvant in this field can add,
Auxiliary material can be used alone, and can also be used in combination of two or more.In some preferred embodiments, the auxiliary material includes
Moisturizer and solubilizer.Radix Sangusorbae extract can be synergistic with moisturizer, with certain guarantor while promoting anti-corrosion effect
Wet performance, solubilizer can be effectively improved the dissolubility of Radix Sangusorbae extract and Tianshan Mountains violet extract in anticorrosive composite, thus
Improve its stability.
The moisturizer for example can be but be not limited to polyalcohols, natural moisturizing factor or amino acid, some excellent
In the embodiment of choosing, moisturizer is preferably polyalcohols moisturizer, and the polyalcohol preferably includes Sensiva SC50, Xin Gan
Alcohol, 1,2- pentanediol or 1, one of 2- hexylene glycol or two kinds more preferably include Sensiva SC50 and pungent glycol or 1,
2- pentanediol and 1,2- hexylene glycol.In some preferred embodiments, the solubilizer is non-ionic solubilizer, the increasing
Solvent includes one of Cremophor RH40, PEG-60 rilanit special or polyvinylpyrrolidone or a variety of, preferably
Including Cremophor RH40.
In some preferred embodiments, the anticorrosive composite for cosmetics includes the following raw material: garden burnet mentions
Take object, Tianshan Mountains violet extract, polyalcohol, solubilizer and deionized water as moisturizer.Anti-corrosion group comprising above-mentioned raw materials
Closing object has the characteristics that irritation is low, stability is high.Radix Sangusorbae extract and the polyalcohol as moisturizer are synergistic, are promoted anti-
With certain performance of keeping humidity while rotten effect, while there is significant antioxidation, while with anti-corrosion effect
Have both certain senile-resistant efficacy.
In some preferred embodiments, according to mass percent meter, each raw material dosage is as follows: Radix Sangusorbae extract
0.5%-10%, for example, can be but be not limited to 0.5%, 1%, 1.5%, 2%, 2.5%, 3%, 3.5%, 4%, 4.5%,
5%, 5.5%, 6%, 6.5%, 7%, 7.5%, 8%, 8.5%, 9%, 9.5% or 10%；Tianshan Mountains violet extract 1.5%-
15%, for example, can be but be not limited to 1.5%, 2%, 2.5%, 3%, 3.5%, 4%, 4.5%, 5%, 5.5%, 6%,
6.5%, 7%, 7.5%, 8%, 8.5%, 9%, 9.5%, 10%, 10.5%, 11%, 11.5%, 12%, 12.5%, 13%,
13.5%, 14%, 14.5% or 15%；Solubilizer 5%-25%, for example, can be but be not limited to 5%, 6%, 7%, 8%,
9%, 10%, 12%, 14%, 15%, 16%, 18%, 20%, 21%, 23%, 25%；Moisturizer 10%-60%, such as can
Think but be not limited to 10%, 12%, 15%, 18%, 20%, 22%, 25%, 28%, 30%, 32%, 35%, 38%,
40%, 42%, 45%, 48%, 50%, 52%, 55%, 58% or 60%；Surplus is water.
In some preferred embodiments, according to mass percent meter, each raw material dosage is as follows: Radix Sangusorbae extract
1.5%-8%, Tianshan Mountains violet extract 3%-12%, solubilizer 8%-20% and moisturizer 25%-50%, surplus are water.
In some preferred embodiments, according to mass percent meter, each raw material dosage is as follows: Radix Sangusorbae extract
2.5%-7.5%, Tianshan Mountains violet extract 3.5%-10.5%, solubilizer 10%-20% and moisturizer 30%-45%, surplus
For water.The adjustment selected with each raw material dosage by optimizing each raw material can advanced optimize the anti-corrosion effect of anticorrosive composite
Fruit.
The present invention also provides a kind of preparation methods of above-mentioned anticorrosive composite, comprising: by Radix Sangusorbae extract, Tianshan Mountains violet
Extract and optionally auxiliary material mix, and can be obtained the anticorrosive composite, and this method has simple process and easy to operate
Advantage.In some preferred embodiments, Radix Sangusorbae extract and Tianshan Mountains violet extract are first dissolved in deionized water and protected
In the mixture of humectant, adds solubilizer and be uniformly mixed, obtain the anticorrosive composite after isolating insoluble matter.
In some preferred embodiments, anticorrosive composite is prepared with reference to following technological parameter, better effect:
I) deionized water is mixed with polyalcohol, stirring is warming up to 40-60 DEG C；
II) Radix Sangusorbae extract and Tianshan Mountains violet extract, stirring and dissolving, mixing speed 200-600r/min is added；
III) solubilizer is added, it stirs evenly, mixing speed 200-600r/min, keeps the temperature 5-15min；
III) 30 DEG C are cooled to hereinafter, crossing 0.22 μm of miillpore filter to get the composition.
In some preferred embodiments, it is higher to be prepared as follows the available saponin content of Radix Sangusorbae extract
Radix Sangusorbae extract, include the following steps: after pretreatment to extract garden burnet powder, after extracting solution is concentrated again with absorption
Purifying resin obtains Radix Sangusorbae extract.
Wherein, the pretreated technique of garden burnet powder are as follows: the mixed liquor of garden burnet powder and low carbon alcohol solution is carried out low
The high-pressure homogeneous processing of temperature, mean value temperature is 0-10 DEG C, such as can be but be not limited to 0 DEG C, 1 DEG C, 2 DEG C, 3 DEG C, 4 DEG C, 5 DEG C, 6
DEG C, 7 DEG C, 8 DEG C, 9 DEG C or 10 DEG C；Homogenization pressure 2.0-50MPa, for example, can be but be not limited to 2MPa, 3MPa, 4MPa,
5MPa, 6MPa, 8MPa, 10MPa, 12MPa, 15MPa, 18MPa, 20MPa, 25MPa, 30MPa, 35MPa, 40MPa, 45MPa or
50MPa；Homogenizing time is 10-60s, for example, can be but be not limited to 10s, 15s, 20s, 25s, 30s, 35s, 40s, 45s,
50s, 55s or 60s.
In some alternative embodiments, low-carbon alcohols for example can be but be not limited to methanol, ethyl alcohol, propyl alcohol, positive fourth
Alcohol or isopropanol, preferably ethyl alcohol；The concentration of low-carbon alcohols is preferably 40%-95%, such as can be but be not limited to 40%,
45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85% or 90%.
Cryogenic high pressure homogeneous technology is applied to the pre-treatment of garden burnet, can destruction to avoid pre-treatment to active principle,
And the high-pressure homogeneous dissolution for effectively facilitating target components, compared with prior art, which can effectively improve extraction efficiency.
Radix Sangusorbae extract is purified using purification on adsorbent resins technology, containing for total saposins in obtained Radix Sangusorbae extract can be promoted
Amount, and interfering component is effectively separated.
In some preferred embodiments, extracting mode be water-bath extraction, ultrasound assisted extraction or Microwave Extraction, preferably
Use ultrasound assisted extraction.Garden burnet is extracted using ultrasonic wave extraction, cavitation, the mechanical oscillation effect of ultrasonic wave
It should can be obviously improved extraction efficiency with fuel factor, while reduce extraction time and reducing extraction process and target components are broken
It is bad.
In some preferred embodiments, the technological parameter of ultrasound assisted extraction is ultrasonic power 228W-380W, such as
Can be but be not limited to 228W, 235W, 240W, 245W, 250W, 260W, 270W, 280W, 290W, 300W, 310W, 320W,
330W, 340W, 350W, 360W, 370W or 380W；Solid-liquid ratio 1:10-1:50, for example, can be but be not limited to 1:10,1:15,
1:20,1:25,1:30,1:35,1:40,1:45 or 1:50；20-55 DEG C of extract temperature, such as can be but be not limited to 20
DEG C, 25 DEG C, 30 DEG C, 35 DEG C, 40 DEG C, 45 DEG C, 50 DEG C or 55 DEG C；Extraction time 0.15-1h, such as can be but be not limited to
0.15h, 0.3h, 0.5h, 0.6h, 0.7h, 0.8h, 0.9h or 1h.
In some preferred embodiments, the absorption resin is macroporous absorbent resin, preferably low pole or non-pole
The macroporous absorbent resin of property；Preferably, the model of macroporous absorbent resin is preferably HPD-100, D101, D4020 or PD-400.Greatly
Macroporous adsorbent resin can the saponin(e of Yuzhong over the ground selectively adsorbed, while removing the sugar in extracting solution, albumen and portion
The unstable components such as color separation element, to effectively improve the purity of garden burnet extracting solution.
In some preferred embodiments, decolorization is carried out to the eluent after purification on adsorbent resins, preferably made
Decolorization is carried out with active carbon, it is of light color by adsorbing the Radix Sangusorbae extract of purifying and active carbon decolorization of resin, it shows
Work improves the saturate problem of current plant extracts.The dosage of active carbon is preferably the 0.2%-2% of eluent quality, example
It such as can be but be not limited to 0.2%, 0.3%, 0.5%, 0.8%, 1.0%, 1.2%, 1.5%, 1.8% or 2%；When decoloration
Between 0.2-1.0h, such as can be but be not limited to 0.2h, 0.3h, 0.5h, 0.8h or 1.0h；30-55 DEG C of bleaching temperature, such as
It can be but be not limited to 30 DEG C, 32 DEG C, 35 DEG C, 38 DEG C, 40 DEG C, 42 DEG C, 45 DEG C, 50 DEG C or 55 DEG C.Active carbon is for system
The absorption of substance does not have selectivity, and the too low decolorizing effect of additive amount is bad, will affect its adsorbing contaminant yet, adding too much,
Although good decolorizing effect, adsorbance increases, and can adsorb more target components, causes the loss of system active principle excessive.
In some preferred embodiments, total saponin content is not low in the Radix Sangusorbae extract for preparing in accordance with the following steps
In 80%, by optimizing and adjusting the technological parameter in following preparation method, Radix Sangusorbae extract can be advanced optimized and adjusted
Preparation effect.The preparation method is carried out with reference to following steps:
S1. the garden burnet after drying is removed into impurity, and is crushed with plant pulverizer, crossed stainless steel mesh screen, obtain Radix Sangusorbae powder,
Crossing grit number is -100 mesh of 30 mesh, preferably 60-80 mesh.
S2. certain density ethanol water is added in garden burnet powder, it is located in advance using cryogenic high pressure homogenizer
Reason.Wherein, mean value temperature is 0-10 DEG C, homogenization pressure 2.0-50MPa, homogenizing time 10-60s, second in ethanol water
The concentration of alcohol is 40%-95%.In one preferred embodiment, according to following technological parameter carry out, extraction effect more preferably:
The concentration 75%-95% of ethyl alcohol in ethanol water, 4 DEG C of homogenizing temperature, homogenization pressure 5.5MPa, homogenizing time 30s.
S3. the mixed liquor after S2 is processed is extracted with ultrasonic wave assisted extraction method, is centrifuged or filters after extraction, collect filter
Liquid.Wherein, ultrasonic power 228W-380W, solid-liquid ratio 1:10-1:50,20-55 DEG C of extract temperature, extraction time 0.15-1h.
In one preferred embodiment, according to following technological parameter carry out, extraction effect more preferably: ultrasonic power 304W-342W, material
Liquor ratio 1:15-1:35,35-50 DEG C of Extracting temperature, extraction time 0.5-1h.
S4. the S3 filtrate obtained is subjected to the 1/10-1/2 for being concentrated under reduced pressure into original volume, obtains Concentrated suspensions, preferably depressurizes
It is concentrated into the 1/5-1/8 of original volume.
S5. the S4 Concentrated suspensions obtained are purified by macroporous absorbent resin, obtains eluent；Preferably, greatly
Macroporous adsorbent resin is low pole or non-polar macroporous resin, and resin model is preferably HPD-100, D101, D4020 or PD-400.
Optionally, eluting solvent is the ethanol water of 60%-90%, elution amount 1BV-4BV, elution speed 2BV/h-4BV/h.
In one preferred embodiment, according to following technological parameter carry out, extraction effect more preferably: macroporous absorbent resin
For low pole macroporous absorbent resin, macroreticular resin HPD-400, eluent is 70%-90% ethanol water, is washed
De- amount is 1.5BV-3BV, elution speed 1.0-2.0BV/h.
S6. active carbon is added in eluent S5 obtained and carries out decolorization, obtains destainer；Activated carbon addition is
0.2%-2%, the bleaching time 0.2-1.0h of eluent quality obtained by S5,30-55 DEG C of bleaching temperature.In a preferred implementation
In mode, according to following technological parameter carry out, extraction effect more preferably: activated carbon addition be eluent quality 0.5%-
1.5%, bleaching time 0.2-0.8h, 40-45 DEG C of bleaching temperature.
S7. the S6 destainer obtained is subjected to the 1/5-1/15 for being concentrated under reduced pressure into original volume, to remove unnecessary alcohol, preferably
It is concentrated under reduced pressure into the 1/6-1/10 of original volume.
S8. the S7 concentrate obtained is freeze-dried, obtains Radix Sangusorbae extract；Wherein it is freeze-dried condition are as follows: cold
Freeze temperature -70~-90 DEG C, sublimation drying 20-70h, vacuum pressure 1.0Pa-15Pa.In a preferred embodiment
In, carried out according to following technological parameter, extraction effect more preferably: it is cryogenic temperature -80~-90 DEG C, sublimation drying 40-60h, true
Pneumatics power 1.0Pa-5.0Pa.
In some preferred embodiments, the Tianshan Mountains available general flavone of violet extract is prepared as follows to contain
Higher Tianshan Mountains violet extract is measured, includes the following steps: after pretreatment to extract Tianshan Mountains violet powder, extracting solution warp
Silica gel column purification is used after concentration again, obtains Tianshan Mountains violet extract.
Wherein, the pretreated technique of Tianshan Mountains violet powder are as follows: the mixing to Tianshan Mountains violet powder and low carbon alcohol solution
Liquid carries out homogenization, and mean value temperature is 0-10 DEG C, such as can be but be not limited to 0 DEG C, 1 DEG C, 2 DEG C, 3 DEG C, 4 DEG C, 5 DEG C, 6
DEG C, 7 DEG C, 8 DEG C, 9 DEG C or 10 DEG C；Homogenization pressure is 2.0-50MPa, for example, can be but be not limited to 2MPa, 3MPa, 4MPa,
5MPa, 6MPa, 8MPa, 10MPa, 12MPa, 15MPa, 18MPa, 20MPa, 25MPa, 30MPa, 35MPa, 40MPa, 45MPa or
50MPa；Homogenizing time is 10-60s, for example, can be but be not limited to 10s, 15s, 20s, 25s, 30s, 35s, 40s, 45s,
50s, 55s or 60s.
In some alternative embodiments, low-carbon alcohols for example can be but be not limited to methanol, ethyl alcohol, propyl alcohol, positive fourth
Alcohol or isopropanol, preferably ethyl alcohol；The concentration of low-carbon alcohols is preferably 35%-95%, such as can be but be not limited to 35%,
40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90% or 95%.
Cryogenic high pressure homogeneous technology is applied to the pre-treatment of Tianshan Mountains violet, active principle can be broken to avoid pre-treatment
It is bad, and the high-pressure homogeneous dissolution for effectively facilitating target components, compared with prior art, which can effectively improve extraction effect
Rate.Tianshan Mountains violet extract is purified using silicagel column purification technique, Tianshan Mountains violet extractive total flavone can be promoted
Content, and interfering component is effectively separated.
In some preferred embodiments, extracting mode be water-bath extraction, ultrasound assisted extraction or Microwave Extraction, preferably
Use ultrasound assisted extraction.Tianshan Mountains violet is extracted using ultrasonic wave extraction, the cavitation of ultrasonic wave, mechanical vibration
Dynamic effect and fuel factor can be obviously improved extraction efficiency, while reducing extraction time and reducing extraction process to target components
It destroys.
In some preferred embodiments, ultrasonic power 228W-380W, for example, can be but be not limited to 228W,
235W、240W、245W、250W、260W、270W、280W、290W、300W、310W、320W、330W、340W、350W、360W、
370W or 380W；Solid-liquid ratio 1:15-1:60, for example, can be but be not limited to 1:15,1:20,1:25,1:30,1:35,1:40,
1:45,1:50,1:55 or 1:60；30-60 DEG C of extract temperature, such as can be but be not limited to 30 DEG C, 35 DEG C, 40 DEG C, 45
DEG C, 50 DEG C, 55 DEG C or 60 DEG C；Extraction time 0.15-1.5h, for example, can be but be not limited to 0.15h, 0.3h, 0.5h,
0.6h, 0.7h, 0.8h, 0.9h, 1h, 1.15h, 1.3h or 1.5h.
In some preferred embodiments, the specification of the silica gel in silicagel column is preferably 100-200 mesh, 200-300 mesh
Or 300-400 mesh.Silica gel effectively can be adsorbed and be parsed to flavones, to remove the pectin in extracting solution, albumen, sugar
Other impurity such as class and partial pigment are obviously improved the content and stability of general flavone in the violet extract of Tianshan Mountains.
In some preferred embodiments, the eluent using ultrafiltration membrance filter through silicagel column after purification obtains ultrafiltration
Filtrate.When liquid mixture flows through film surface under a certain pressure, small molecule solute penetrates film, and macromolecular substances are then cut
It stays, macromolecular concentration in stoste is gradually increased, to realize the purpose of the separation of large and small molecule, concentration, purification.Using super
Tianshan Mountains violet extract can be further purified in filter membrane, remove part macromolecular substances, and Tianshan Mountains violet general flavone can penetrate
Ultrafiltration membrane, to achieve the purpose that purifying.And face can be obtained through the eluent of silicagel column after purification using ultrafiltration membrance filter
The shallower Tianshan Mountains violet extract of color, significantly improves the saturate problem of current plant extracts.Hyperfiltration technique is to pass through film
The microcellular structure on surface carries out Selective Separation to substance.Wherein ultrafiltration membrane aperture is preferably 1KD or 5KD or 10KD.
In some preferred embodiments, general flavone content in the Tianshan Mountains violet extract for preparing in accordance with the following steps
Not less than 85%, by optimizing and adjusting the technological parameter in the preparation method, Tianshan Mountains violet can be advanced optimized and adjusted
The preparation effect of extract.The preparation method is carried out with reference to following steps:
S1. the Tianshan Mountains violet after drying is removed into impurity, and is crushed with plant pulverizer, crossed stainless steel mesh screen, obtain day
Mountain violet powder, crossing grit number is -80 mesh of 30 mesh, -60 mesh of preferably 50 mesh.
S2. Tianshan Mountains violet powder is added in certain density ethanol water, using cryogenic high pressure homogenizer to its into
Row pretreatment.Wherein, 0-10 DEG C of homogenizing temperature, homogenization pressure 2.0-50MPa, homogenizing time 10-60s；Second in ethanol water
The concentration of alcohol is 35%-95%.In one preferred embodiment, according to following technological parameter carry out, extraction effect more preferably:
The concentration 70%-80% of ethanol water ethyl alcohol, 4 DEG C of homogenizing temperature, homogenization pressure 5.5MPa, homogenizing time 30s.
S3. the mixed liquor after S2 is processed is extracted with ultrasonic wave assisted extraction method, is centrifuged or filters after extraction, collect filter
Liquid；Wherein, ultrasonic power 228W-380W, solid-liquid ratio 1:15-1:60,30-60 DEG C of extract temperature, extraction time 0.15-
1.5h.In one preferred embodiment, according to following technological parameter carry out, extraction effect more preferably: ultrasonic power 266W-
342W, solid-liquid ratio 1:20-1:30,40-45 DEG C of Extracting temperature, extraction time 0.5-1.0h.
S4. the S3 filtrate obtained is subjected to the 1/8-1/3 for being concentrated under reduced pressure into original volume, is preferably concentrated under reduced pressure into original volume
, make wherein general flavone content in 8-15mg/mL.
S5. the S4 Concentrated suspensions obtained are purified by silicagel column, obtains eluent；Silica gel in silicagel column
Specification is preferably 100-200 mesh, 200-300 mesh or 300-400 mesh.Optionally, eluting solvent is that the ethyl alcohol of 50%-85% is water-soluble
Liquid, elution amount 0.5BV-3.5BV, elution flow rate 1.5BV/h-3BV/h.In one preferred embodiment, according to such as
Lower technological parameter carries out, extraction effect more preferably: the specification of used silica gel is 200-300 mesh, and eluent is 80%-90% ethyl alcohol,
Elution amount is 2BV-3BV, elution flow rate 1.5-2BV/h.
S6. it will be handled in the eluent of S5 acquisition by ultrafiltration membrane, obtain ultrafiltrate.Skill is purified using ultrafiltration membrane
Art can purify the general flavone in the violet of Tianshan Mountains, promote the purity of obtained extract.Wherein ultrafiltration membrane aperture is preferably
1KD or 5KD or 10KD；Ultrafiltration pressure 5psi-20psi.In one preferred embodiment, according to following technological parameter into
Row, extraction effect more preferably: the aperture of ultrafiltration membrane be 5KD or 10KD, ultrafiltration pressure 5psi-15psi.
S7. the 1/4-1/15 that the S6 ultrafiltrate obtained be concentrated under reduced pressure into original volume is obtained into concentrate, preferably subtracted
Pressure is concentrated into the 1/5-1/12 of original volume.
S8. the S7 concentrate obtained is freeze-dried, obtains Tianshan Mountains violet extract and is wherein freeze-dried condition are as follows:
Cryogenic temperature -70~-90 DEG C, sublimation drying 20-70h, vacuum pressure 1.0Pa-15Pa.In a preferred embodiment
In, carried out according to following technological parameter, extraction effect more preferably: it is cryogenic temperature -80~-90 DEG C, sublimation drying 40-60h, true
Pneumatics power 1.0Pa-5.0Pa.
The present invention also provides a kind of above-mentioned anticorrosive composites to prepare the purposes in cosmetics, which has
Widely used advantage can be applied in the cosmetic product of multiple functions and diversified forms, and above-mentioned anticorrosive composite can be applied
In skin care item, color make-up or medicine adornment；It can also be applied to skin care cream, moisturizing lotion, moisture retention water, facial treatment essence, mildy wash or face
In the product of the diversified forms such as film.
It is effective due to above-mentioned anticorrosive composite the present invention also provides a kind of cosmetics comprising above-mentioned anticorrosive composite
Ingredient is all natural component, therefore while have the advantages that be not easy putrid and deteriorated using its cosmetics, is also had lower
Sensitization.It is understood that cosmetics of the present invention for example can be but be not limited to reach cleaning, maintenance, beauty
Hold, modification and change appearance etc. various types of skin care item, color make-up or medicine adornment.In some alternative embodiments, describedization
Cosmetic for example can be but be not limited to skin care cream, moisturizing lotion, moisture retention water, facial treatment essence, mildy wash or facial mask.
Beneficial effects of the present invention are further illustrated below with reference to preferred embodiment.
Embodiment 1
A kind of preparation method of long fringe Radix Sangusorbae extract, specifically includes the following steps:
(S1) root of the long fringe garden burnet after drying is removed into impurity, and is crushed with plant pulverizer, cross 60 mesh stainless (steel) wires
Sieve, obtains long fringe Radix Sangusorbae powder.
(S2) long fringe garden burnet is added in the ethanol water of 85% concentration, it is carried out using cryogenic high pressure homogenizer pre-
Processing, 4 DEG C of homogenizing temperature, homogenization pressure 5.5MPa, homogenizing time 30s.
(S3) it is extracted using ultrasonic wave assisted extraction method, ultrasonic power 342W, solid-liquid ratio 1:20, are mentioned by 40 DEG C of Extracting temperature
Time 0.5h is taken, is centrifuged or filters after extraction, collects filtrate.
(S4) filtrate of acquisition is carried out being concentrated under reduced pressure into the 1/5 of original volume, obtains Concentrated suspensions.
(S5) Concentrated suspensions are purified by macroporous absorbent resin, macroreticular resin HPD-400 is washed
De- liquid is 75% ethanol water, elution amount 1.5BV, elution speed 1.5BV/h.
(S6) active carbon will be added in the eluent of acquisition and carries out decolorization, activated carbon addition 0.5%, when decoloration
Between 0.5h, 40 DEG C of bleaching temperature.
(S7) destainer of acquisition is carried out being concentrated under reduced pressure into the 1/7 of original volume, removes unnecessary alcohol.
(S8) concentrate of acquisition is freeze-dried, -85 DEG C of cryogenic temperature, sublimation drying 45h, vacuum pressure
1.0Pa。
Embodiment 2
A kind of preparation method of long fringe Radix Sangusorbae extract, specifically includes the following steps:
(S1) root of the long fringe garden burnet after drying is removed into impurity, and is crushed with plant pulverizer, cross 80 mesh stainless (steel) wires
Sieve, obtains long fringe Radix Sangusorbae powder.
(S2) long fringe garden burnet is added in the ethanol water of 90% concentration, it is carried out using cryogenic high pressure homogenizer pre-
Processing, 4 DEG C of homogenizing temperature, homogenization pressure 5.5MPa, homogenizing time 30s.
(S3) it is extracted using ultrasonic wave assisted extraction method, ultrasonic power 304W, solid-liquid ratio 1:30, are mentioned by 40 DEG C of Extracting temperature
Time 0.75h is taken, is centrifuged or filters after extraction, collects filtrate.
(S4) filtrate of acquisition is carried out being concentrated under reduced pressure into the 1/6 of original volume, obtains Concentrated suspensions.
(S5) Concentrated suspensions are purified by macroporous absorbent resin, macroreticular resin HPD-400 is washed
De- liquid is 85% ethanol water, elution amount 2.0BV, elution speed 1.5BV/h.
(S6) active carbon will be added in the eluent of acquisition and carries out decolorization, activated carbon addition 0.75%, decoloration
Time 0.7h, 45 DEG C of bleaching temperature.
(S7) destainer of acquisition is carried out being concentrated under reduced pressure into the 1/6 of original volume, removes unnecessary alcohol.
(S8) concentrate of acquisition is freeze-dried, -90 DEG C of cryogenic temperature, sublimation drying 56h, vacuum pressure
1.5Pa。
Embodiment 3
A kind of preparation method of Tianshan Mountains violet extract, specifically includes the following steps:
(S1) the Tianshan Mountains violet after drying is removed into impurity, and is crushed with plant pulverizer, crossed 50 mesh stainless steel mesh screens, obtain
To Tianshan Mountains violet powder.
(S2) Tianshan Mountains violet powder is added in the ethanol water of 80% concentration, it is carried out using cryogenic high pressure homogenizer
Pretreatment, 4 DEG C of homogenizing temperature, homogenization pressure 5.5MPa, homogenizing time 30s.
(S3) it is extracted using ultrasonic wave assisted extraction method, ultrasonic power 304W, solid-liquid ratio 1:30, are mentioned by 45 DEG C of Extracting temperature
Time 0.5h is taken, is centrifuged or filters after extraction, collects filtrate.
(S4) filtrate of acquisition is carried out being concentrated under reduced pressure into the 1/4 of original volume, obtains Concentrated suspensions.
(S5) Concentrated suspensions are purified by silicagel column, used silica gel mesh number is 200-300 mesh, and eluent is
85% ethyl alcohol, elution amount 2BV, elution flow rate 1.5BV/h.
(S6) eluent of acquisition is purified with cross-flow ultrafiltration system, the aperture of ultrafiltration membrane is 5KD, ultrafiltration pressure
5psi。
(S7) ultrafiltrate of acquisition is carried out being concentrated under reduced pressure into the 1/8 of original volume, removes unnecessary alcohol.
(S8) concentrate of acquisition is freeze-dried, -85 DEG C of cryogenic temperature, sublimation drying 50h, vacuum pressure
3.0Pa。
Embodiment 4
A kind of preparation method of Tianshan Mountains violet extract, specifically includes the following steps:
(S1) the Tianshan Mountains violet after drying is removed into impurity, and is crushed with plant pulverizer, crossed 60 mesh stainless steel mesh screens, obtain
To Tianshan Mountains violet powder.
(S2) Tianshan Mountains violet powder is added in the ethanol water of 70% concentration, it is carried out using cryogenic high pressure homogenizer
Pretreatment, 4 DEG C of homogenizing temperature, homogenization pressure 5.5MPa, homogenizing time 30s.
(S3) it is extracted using ultrasonic wave assisted extraction method, ultrasonic power 266W, solid-liquid ratio 1:20, are mentioned by 40 DEG C of Extracting temperature
Time 1.0h is taken, is centrifuged or filters after extraction, collects filtrate.
(S4) filtrate of acquisition is carried out being concentrated under reduced pressure into the 1/5 of original volume, obtains Concentrated suspensions.
(S5) Concentrated suspensions are purified by silicagel column, used silica gel mesh number is 200-300 mesh, and eluent is
90% ethyl alcohol, elution amount 3BV, elution flow rate 2BV/h.
(S6) eluent of acquisition is purified with cross-flow ultrafiltration system, the aperture of ultrafiltration membrane is 10KD, ultrafiltration pressure
Power 10psi.
(S7) ultrafiltrate of acquisition is carried out being concentrated under reduced pressure into the 1/10 of original volume, removes unnecessary alcohol.
(S8) concentrate of acquisition is freeze-dried, -90 DEG C of cryogenic temperature, sublimation drying 60h, vacuum pressure
4.0Pa。
Embodiment 5
A kind of anticorrosive composite is present embodiments provided, specifically includes following raw material: long fringe Radix Sangusorbae extract 5%, Tianshan Mountains
Violet extract 7%, Cremophor RH40 14%, pungent glycol 15% and Sensiva SC50 20%, surplus are deionization
Water.Wherein, long fringe Radix Sangusorbae extract is that embodiment 1 is prepared, and Tianshan Mountains violet extract is that embodiment 3 is prepared.
Embodiment 6
A kind of anticorrosive composite is present embodiments provided, specifically includes following raw material: long fringe Radix Sangusorbae extract 3%, Tianshan Mountains
Violet extract 9%, Cremophor RH40 20%, pungent glycol 25% and Sensiva SC50 15%, surplus are deionization
Water.Wherein, long fringe Radix Sangusorbae extract is that embodiment 2 is prepared, and Tianshan Mountains violet extract is that embodiment 4 is prepared.
Embodiment 7
A kind of anticorrosive composite is present embodiments provided, specifically includes following raw material: long fringe Radix Sangusorbae extract 1.5%, day
Mountain violet extract 12%, polyvinylpyrrolidone 8%, pungent glycol 25%, Sensiva SC50 25%, surplus are deionization
Water.Wherein, long fringe Radix Sangusorbae extract is that embodiment 1 is prepared, and Tianshan Mountains violet extract is that embodiment 3 is prepared.
Embodiment 8
A kind of anticorrosive composite is present embodiments provided, specifically includes following raw material: long fringe Radix Sangusorbae extract 8%, Tianshan Mountains
Violet extract 3%, Cremophor RH40 20%, pungent glycol 15% and 1,2- pentanediol 10%, surplus are deionized water.
Wherein, long fringe Radix Sangusorbae extract is that embodiment 1 is prepared, and Tianshan Mountains violet extract is that embodiment 3 is prepared.
Embodiment 9
A kind of anticorrosive composite is present embodiments provided, specifically includes following raw material: long fringe Radix Sangusorbae extract 0.5%, day
Mountain violet extract 15%, PEG-60 rilanit special 5%, 1,2- hexylene glycol 30% and Sensiva SC50 30%, surplus are
Deionized water.Wherein, long fringe Radix Sangusorbae extract is that embodiment 1 is prepared, and Tianshan Mountains violet extract is that embodiment 3 is prepared into
It arrives.
Embodiment 10
A kind of anticorrosive composite is present embodiments provided, specifically includes following raw material: long fringe Radix Sangusorbae extract 10%, Tianshan Mountains
Violet extract 1.5%, Cremophor RH40 25%, pungent glycol 5% and Sensiva SC50 5%, surplus are deionization
Water.Wherein, long fringe Radix Sangusorbae extract is that embodiment 1 is prepared, and Tianshan Mountains violet extract is that embodiment 3 is prepared.
Embodiment 11
A kind of anticorrosive composite is present embodiments provided, specifically includes following raw material: long fringe Radix Sangusorbae extract 15%, Tianshan Mountains
Violet extract 1%, Cremophor RH40 30%, pungent glycol 2.5% and Sensiva SC50 2.5%, surplus be go from
Sub- water.Wherein, long fringe Radix Sangusorbae extract is that embodiment 1 is prepared, and Tianshan Mountains violet extract is that embodiment 3 is prepared.
Embodiment 12
A kind of anticorrosive composite is present embodiments provided, specifically includes following raw material: long fringe Radix Sangusorbae extract 15%, Tianshan Mountains
Violet extract 1%, Cremophor RH40 5%, pungent glycol 25% and Sensiva SC50 25%, surplus are deionization
Water.Wherein, long fringe Radix Sangusorbae extract is that embodiment 1 is prepared, and Tianshan Mountains violet extract is that embodiment 3 is prepared.
Comparative example 1
This comparative example provides a kind of anticorrosive composite, specifically includes following raw material: long fringe Radix Sangusorbae extract 7.5%,
Cremophor RH40 7%, 1,2- pentanediol 28% and 1,2- hexylene glycol 10%, surplus are deionized water, wherein long fringe
Elm extract is that embodiment 1 is prepared.
Comparative example 2
This comparative example provides a kind of anticorrosive composite, specifically includes following raw material: long fringe Radix Sangusorbae extract 12%, PEG-
40 rilanit specials 8%, 1,2- pentanediol 32% and 1,2- hexylene glycol 12%, surplus are deionized water, wherein long fringe garden burnet mentions
Taking object is that embodiment 2 is prepared.
Comparative example 3
This comparative example provides a kind of anticorrosive composite, specifically includes following raw material: Tianshan Mountains violet extract 12%, PEG-
40 rilanit specials 5%, pungent glycol 15% and Sensiva SC50 10%, surplus are deionized water, wherein Tianshan Mountains violet extracts
Object is that embodiment 3 is prepared.
Comparative example 4
This comparative example provides a kind of anticorrosive composite, specifically includes following raw material: Tianshan Mountains violet extract 7%, PEG-
40 rilanit specials 10%, pungent glycol 20% and Sensiva SC50 15%, surplus are deionized water, wherein Tianshan Mountains violet mentions
Taking object is that embodiment 4 is prepared.
Effect example 1: antiseptic property is investigated --- MIC experiment
It is tested with embodiment 5-12 and comparative example 1-4, tests its minimal inhibitory concentration MIC value: after sterilizing
Nutrient broth, sabouraud culture medium are dilution, are diluted using doubling dilution to test sample, are connect in backward dilution
Kind, the bacterial concentration of inoculation is respectively as follows: escherichia coli 1 × 108Cfu/mL, staphylococcus aureus 1 × 108Cfu/mL, copper
Green pseudomonad 1 × 107Cfu/mL, Candida albicans 1 × 106Cfu/mL, aspergillus niger 1 × 105cfu/mL.35 DEG C of culture 36h
(bacterium), 28 DEG C of culture 48h (fungi) observe result afterwards.
Bacterium uses trace liquid diluting method, and the 3h before cultivating terminal is added TTC reagent, continues to cultivate, if culture solution becomes
It is red, then assert that this concentration cannot suppress growth of microorganism, if culture solution does not redden, regards as in the culture solution not reddened most
Small drug concentration minimal inhibitory concentration of the bacteriostatic agent to this microorganism thus.Concrete outcome is as shown in table 1.
1 embodiment of table and comparative example MIC value
The embodiment of the present invention all has good fungistatic effect to above-mentioned various bacteriums it can be seen from 1 result of table, and compares
The fungistatic effect of example is poor.
Effect example 2: antiseptic property is investigated --- Bactericidal test
It is tested with embodiment 5-12 and comparative example 1-4.With staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli,
Candida albicans are tested using K~B paper disk method as inoculation bacterium to compare the difference of bacteriostasis between them.It is specific real
Proved recipe method is as follows:
By Escherichia coli, staphylococcus aureus, Pseudomonas aeruginosa is inoculated with respectively on MH fluid nutrient medium, is cultivated at 37 DEG C
24h；Candida albicans inoculation is husky to protect weak fluid nutrient medium, cultivates 5d at 25 DEG C.With physiological saline by four kinds of bacterial cultures
Being diluted to concentration respectively is 1.0 × 106The bacteria suspension of CFU/ml.
Above-mentioned four kinds of bacteria suspensions are coated on nutrient agar panel respectively with aseptic cotton carrier, lawn is made, then takes diameter
For the sterilizing filtrate disk of 3cm.Example and the sample of comparative example respectively, then be attached to four kinds of different bacteria suspensions respectively and be made
Plate on.For 24 hours, fungal culture 5d observes result to Bacteria Culture.Paper disk method bacteriostatic diameter >=10mm is effective, bacteriostatic diameter
≤ 10mm be it is invalid, experimental result is as shown in table 2.
2 paper disk method of table measures each sample anti-corrosion effect
Antiseptic composition obtained by the present invention has a preferable fungistatic effect it can be seen from 2 result of table, and comparative example institute
It is poor to obtain composition fungistatic effect.
Effect example 3: antiseptic property is investigated --- preservation challenge experiment
It is tested with embodiment 5-12 and comparative example 1-4.With reference to U.S.'s cosmetics, toilet articles and essence association
(CTFA) the protective system efficiency evaluation method recommended carries out 28 days singles and connects the experiment of bacterium challenge.
(1) experimental strain: bacterium (Escherichia coli, staphylococcus aureus, pseudomonas aeruginosa；Fungi (aspergillus niger, expansion
Penicillium patulum).It is inoculated with using Mixed Microbes, bacterium Mixed Microbes suspension 107CFU/mL；Fungi Mixed Microbes suspension 106CFU/mL。
(2) sample inoculation: weighing the various embodiments described above and each 100 grams each 2 parts of comparative example respectively, is separately added into 1mL mixing
Bacterial suspension and 1mL mixed fungus suspension are uniformly mixed, and sample is saved in (22.5 ± 2.5 DEG C) of room temperature, and respectively at
0, the clump count in 2,7,14,21,28 days detection cosmetics, to judge the inhibitor effectiveness of cosmetics.
(3) experimental result: as shown in table 3.
3 preservation challenge result of table
Embodiment was in the 7th day preservation challenge by bacterium it can be seen from the experimental result of table 3, and each embodiment is logical
The number of days for crossing fungal preservation challenge is the 14th day, the good anti-corrosion effect of the results show obtained composition of the invention
Fruit.
Effect example 4: sensitization is investigated --- patch experiment
It is investigated with the sensitization of the sample of embodiment 5-12 and comparative example 1-4, using 30 subjects as object reality
Apply patch test.Area is selected to be no more than 50mm2, depth about 1mm qualified spot tester.Tested material is put into spot tester cell
Interior, dosage is about 0.020g, and control wells are blank control (not setting any substance).It will be added with the low sensitization of the spot tester of tested material
Adhesive tape is pasted on the back or forearm song side of subject, is gently pressed with palm and is allowed to equably be pasted on skin, continued for 24 hours.Point
Not after removing tested material spot tester 30min (after impression disappearance after), for 24 hours with 48h observe dermoreaction, experimental result such as table 4
It is shown.
Effect example 5: study on the stability
The stability of embodiment and comparative example is investigated respectively, stabilization is evaluated in observation at various temperatures over 1 month
Property.Experimental result is as shown in table 5.
| Room temperature | 4℃ | -20℃ | Illumination | 40℃ | Hot and cold alternation | |
| Embodiment 5 | Stablize | Stablize | Stablize | Stablize | Stablize | Stablize |
| Embodiment 6 | Stablize | Stablize | Stablize | Stablize | Stablize | Stablize |
| Embodiment 7 | Stablize | Stablize | Stablize | Stablize | Stablize | Stablize |
| Embodiment 8 | Stablize | Stablize | Stablize | Stablize | Stablize | Stablize |
| Embodiment 9 | Stablize | Stablize | Stablize | Stablize | Stablize | Stablize |
| Embodiment 10 | Stablize | Stablize | Stablize | Stablize | Stablize | Stablize |
| Embodiment 11 | Stablize | Stablize | Stablize | Stablize | Stablize | Stablize |
| Embodiment 12 | Stablize | Stablize | Stablize | Stablize | Stablize | Stablize |
| Comparative example 1 | Stablize | Stablize | Stablize | Stablize | Stablize | Stablize |
| Comparative example 2 | Stablize | Stablize | Stablize | Stablize | Stablize | Stablize |
| Comparative example 3 | Stablize | Stablize | Stablize | Stablize | Stablize | Stablize |
| Comparative example 4 | Stablize | Stablize | Stablize | Stablize | Stablize | Stablize |
Effect example 6: antioxygenic property is investigated --- and DPPH removes experiment
The examination of DPPH Scavenging activity is carried out, with the sample of embodiment 5-12 and comparative example 1-4 to determine its antioxygen
Change performance.
(1) experimental principle
DPPH is also known as 1,1- diphenyl -2- trinitrophenyl-hydrazine, is a kind of free radical at very stable nitrogen center, it steady
The spatial obstacle of qualitative 3 phenyl ring mainly from Resonance Stabilization action, makes azygous electronics on the nitrogen-atoms being clipped in the middle
Its due electronics cannot be played to act in pairs.Its ethanol solution is purple, has absorption maximum at 517nm wavelength,
Absorbance is in a linear relationship with concentration.When free radical scavenger being added thereto, DPPH can be combined or be substituted, free radical is made
Quantity is reduced, and absorbance becomes smaller, and solution colour shoals, the ability for removing free radical evaluable whereby.
(2) preparation of reagents
Prepare the DPPH methanol solution of 0.2mmol/L；The VC solution for preparing 0.1% each sample aqueous solution and 1mg/mL is made
For positive control sample.
(3) experimental procedure
2mL sample solution+2mLDPPH solution mixes in tool plug test tube, is protected from light 30min, measures 517nm wave
Light absorption value A1 under long；
2mL sample solution+2mL methanol mixes in tool plug test tube, is protected from light 30min, measures under 517nm wavelength
Light absorption value A2；
2mLDPPH solution+2mL methanol mixes in tool plug test tube, is protected from light 30min, measures under 517nm wavelength
Light absorption value A0；
(4) experimental result
6 DPPH Scavenging activity measurement result of table
As shown in table 6, each embodiment has significant scavenging effect to DPPH, it is shown that good anti-oxidation efficacy.
It is excellent to can be seen that present invention composition anti-corrosion effect obtained by the experimental result of embodiment, significantly reduces simultaneously
Sensitisation problems present in plant extracts, while having good stability and there is good oxidation resistance, it is anti-in addition to having
Senile-resistant efficacy raw material is also used as other than rotten effect applied in cosmetics.
Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the present invention., rather than its limitations；To the greatest extent
Pipe present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that: its according to
So be possible to modify the technical solutions described in the foregoing embodiments, or to some or all of the technical features into
Row equivalent replacement；And these are modified or replaceed, various embodiments of the present invention technology that it does not separate the essence of the corresponding technical solution
The range of scheme.
Claims (15)
1. a kind of anticorrosive composite for cosmetics, which is characterized in that the active raw materials of the anticorrosive composite include
Radix Sangusorbae extract and Tianshan Mountains violet extract；The anticorrosive composite further includes moisturizer and solubilizer；
According to mass percent meter, each raw material dosage is as follows: Radix Sangusorbae extract 0.5%-10%, Tianshan Mountains violet extract 1.5%-
15%, solubilizer 5%-25% and moisturizer 10%-60%, surplus are water.
2. anticorrosive composite according to claim 1, which is characterized in that the Radix Sangusorbae extract includes that long fringe garden burnet is extracted
Object.
3. anticorrosive composite according to claim 1, which is characterized in that the content of saponin(e is not low in the Radix Sangusorbae extract
In the 80% of Radix Sangusorbae extract gross mass.
4. anticorrosive composite according to claim 1, which is characterized in that general flavone contains in the Tianshan Mountains violet extract
Amount is not less than the 85% of Tianshan Mountains violet extract gross mass.
5. anticorrosive composite according to claim 1, which is characterized in that the moisturizer includes polyalcohol.
6. anticorrosive composite according to claim 5, which is characterized in that the polyalcohol includes Sensiva SC50, pungent
One of glycol, 1,2- pentanediol or 1,2- hexylene glycol are a variety of.
7. anticorrosive composite according to claim 6, which is characterized in that the moisturizer includes Sensiva SC50 and pungent
Glycol or 1,2- pentanediol and 1,2- hexylene glycol.
8. anticorrosive composite according to claim 1, which is characterized in that the solubilizer is non-ionic solubilizer, institute
Stating solubilizer includes one of Cremophor RH40, PEG-60 rilanit special or polyvinylpyrrolidone or a variety of.
9. anticorrosive composite according to claim 8, which is characterized in that the solubilizer includes PEG-40 hydrogenated castor
Oil.
10. -9 described in any item anticorrosive composites according to claim 1, which is characterized in that according to mass percent meter, each original
Expect that dosage is as follows: Radix Sangusorbae extract 1.5%-8%, Tianshan Mountains violet extract 3%-12%, solubilizer 8%-20% and moisturizer
25%-50%, surplus are water.
11. anticorrosive composite according to claim 10, which is characterized in that according to mass percent meter, each raw material dosage
It is as follows: Radix Sangusorbae extract 2.5%-7.5%, Tianshan Mountains violet extract 3.5%-10.5%, solubilizer 10%-20% and moisturizer
30%-45%, surplus are water.
12. a kind of preparation method of the described in any item anticorrosive composites of claim 1-11 characterized by comprising by ground
Elm extract, Tianshan Mountains violet extract and optionally auxiliary material mixing, obtain the anticorrosive composite.
13. preparation method according to claim 12 characterized by comprising first by Radix Sangusorbae extract and Tianshan Mountains violet
Extract is dissolved in the mixture of deionized water and moisturizer, is added solubilizer and is uniformly mixed, and is obtained after isolating insoluble matter
To the anticorrosive composite.
14. the described in any item anticorrosive composites of claim 1-11 are preparing the purposes in cosmetics.
15. including the cosmetics of the described in any item anticorrosive composites of claim 1-11, which is characterized in that the toiletry bag
Include skin care cream, moisturizing lotion, moisture retention water, facial treatment essence, mildy wash or facial mask.
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