CN108991020A - A kind of extracting method of Hypoxylon fermentation broth extract and its application in mango disease control - Google Patents
A kind of extracting method of Hypoxylon fermentation broth extract and its application in mango disease control Download PDFInfo
- Publication number
- CN108991020A CN108991020A CN201810888964.2A CN201810888964A CN108991020A CN 108991020 A CN108991020 A CN 108991020A CN 201810888964 A CN201810888964 A CN 201810888964A CN 108991020 A CN108991020 A CN 108991020A
- Authority
- CN
- China
- Prior art keywords
- hypoxylon
- fermentation broth
- extract
- mango
- broth extract
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/02—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing liquids as carriers, diluents or solvents
- A01N25/04—Dispersions, emulsions, suspoemulsions, suspension concentrates or gels
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/16—Heavy metals; Compounds thereof
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Environmental Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Wood Science & Technology (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Dentistry (AREA)
- Chemical & Material Sciences (AREA)
- Microbiology (AREA)
- Inorganic Chemistry (AREA)
- Virology (AREA)
- Biotechnology (AREA)
- Mycology (AREA)
- Dispersion Chemistry (AREA)
- Toxicology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Pretreatment Of Seeds And Plants (AREA)
Abstract
The invention discloses a kind of extracting method of Hypoxylon fermentation broth extract and its applications in mango disease control, the extracting method of Hypoxylon fermentation broth extract is that Hypoxylon is placed in culture in culture medium to obtain culture solution, it will be after culture solution filtration sterilization using gauze, primary extract is obtained by extraction using ethyl alcohol, propyl alcohol, ethylene glycol mixture, primary extract progress supercritical extract is obtained into the Hypoxylon fermentation broth extract.Hypoxylon fermentation broth extract is applied to mango pest control method are as follows: Hypoxylon fermentation broth extract is prepared into reagent and is periodically sprinkled in mango, which contains following component: Hypoxylon fermentation broth extract, Kocide SD, glucose, hydrogen peroxide, nonionic surfactant, cationic surfactant, titanium dioxide, lauric acid.Hypoxylon fermentation broth extract of the present invention has stronger bactericidal ability, and the anti-evil of disease pest applied to mango can kill the pathogen for infecting mango, and disease does not occur substantially for mango.
Description
Technical field
The present invention relates to planting fruit trees technical fields, and in particular to a kind of extracting method of Hypoxylon fermentation broth extract and
Its application in mango disease control.
Background technique
Hypoxylon is under the jurisdiction of Ascomycotina (Ascomycotina), gang pyrenomycetes in classification of fungi system
(Pyrenomycetes) Sphaerials (Sphaeriales), Xylariaceae (Xylaiaccac), Hypoxylon category (Hypoxylon).
Currently, the research that related Hypoxylon belongs to is concentrated mainly on two aspects, first is that the disease as caused by Hypoxylon, second is that several in the category
The research and application of kind marine fungi metabolite.
Mango disease mainly has: mango anthracnose, gummosis branch-rot, bacterial black rot and mango graywall, mango worm
Evil mainly has: mango band tail noctuid (being commonly called as tip snout moth's larva or borer), Mango Leaf-hopper (mango brachycephaly leafhopper), the small reality of citrus
Fly, mango ridge chest longicorn, mango cecidomyiia cut leaf weevil, aphid and scale insect.In existing research, the fermentation of Hypoxylon is found
Substrate extract has certain bacteriostatic activity, but the effect and application to Hypoxylon extract are less.Existing mango disease
Pest control mainly uses chemical prevention, less to the research of the sterilizing insect prevention of natural noresidue.
Summary of the invention
The present invention overcomes low to Hypoxylon research and utilization rate in the prior art, and the main useization of the disease control of mango
Learn to farm medicine, there is pesticide residue, there is the technical issues of side effect, provide a kind of Hypoxylon fermentation broth extract extracting method and
Its application in mango disease control.
To solve the above problems, the present invention adopts the following technical scheme that:
A kind of extracting method of Hypoxylon fermentation broth extract, includes the following steps:
(1) culture of Hypoxylon: Hypoxylon is placed in culture medium, and 5~6d is cultivated at 25~28 DEG C and obtains culture solution,
The culture medium prescription is potato 2g, soybean protein 5g, pinus sylvestris var. mongolica fiber 6g, glucose 12g, KH2PO4、MgSO4, water
100ml；
(2) alcohol extracting: using gauze after culture solution filtration sterilization, will use mass ratio for the ethyl alcohol of 10:5:2, propyl alcohol,
Primary extract is obtained by extraction in ethylene glycol mixture, and primary extract is placed in supercritical extract axe and carries out supercritical extract,
30~40min, which is extracted, as entrainer using dehydrated alcohol obtains the Hypoxylon fermentation broth extract；The supercritical carbon dioxide
Extraction temperature is 40~60 DEG C, extracting pressure 70kpa, carbon dioxide flow rate 25L/min.
The object of the invention is also to provide a kind of by the Hypoxylon fermentation broth extract in mango disease control
Application method, concrete application method are that the Hypoxylon fermentation broth extract is prepared into reagent to periodically sprinkle in mango；It is described
Reagent contain mass parts following group be grouped as: 80~100 parts of Hypoxylon fermentation broth extract, 1~3 part of Kocide SD, Portugal
2~5 parts of grape sugar, 1~6 part of hydrogen peroxide, 2~6 parts of nonionic surfactant, 4~8 parts of cationic surfactant, titanium dioxide
0.1~0.3 part of titanium, 1~3 part of lauric acid.
Preferably, the nonionic surfactant is polyol type.
Preferably, the nonionic surfactant is the one or two of sorbitan ester, sucrose ester.
Preferably, the cationic surfactant is quaternary cationics.
Further, the reagent is prepared by the following method: by Hypoxylon fermentation broth extract and anionic surface
Activating agent, cation surface activating, using addition titanium dioxide, lauric acid, hydrogen-oxygen after 10~20min of ultrasonic disperse after agent mixing
Change copper, glucose, hydrogen peroxide, then using 80~90min of mixing under ultrasonic disperse and churned mechanically collective effect to obtain the final product
The reagent.
The present invention has the advantages that compared with prior art
(1) containing stronger bactericidal ability in the Hypoxylon fermentation broth extract that the present invention is prepared, to mango
Various pathogens have killing effect, can be applied in the anti-evil of disease pest of mango, the pathogen for infecting mango can be killed
And disease does not occur substantially for pest, mango.
(2) present invention be prepared by Hypoxylon fermentation broth extract reagent be made be sprayed on mango, mango is carried out
Disease control, in the reagent, sorbitan ester, sucrose ester can provide hydroxyl in nonionic surfactant, quaternary ammonium salt sun from
Sub- surfactant is for positive charge, nonionic surfactant and quaternary cationics and Hypoxylon fermentation liquid
Stable emulsion, quaternary ammonium salt cationic surface-active can be formed after extract, lauric acid, Kocide SD, glucose, hydrogen peroxide mixing
Agent good water solubility, it is not only acidproof but also alkaline-resisting and most of with bactericidal effect, it can be killed with the cooperation of Hypoxylon fermentation broth extract
Most of mango infective pathogen bacterium, copper dioxide, titanium dioxide, hydrogen peroxide can enhance charcoal under the irradiation of sunlight middle-ultraviolet lamp
The ability of kill pathogen in group's fermented liquid extract, bactericidal effect are good, to mango anthracnose, gummosis branch-rot, bacterium
Property black spot, mango graywall there is stronger preventive and therapeutic effect, while to the pest for infecting mango, as mango band tail noctuid (,
Mango Leaf-hopper, dacus dorsalis hendel of mandarin orange, mango ridge chest longicorn, mango cecidomyiia cut leaf weevil, and aphid and scale insect are also different
The toxic action of degree.
Specific embodiment
Below with reference to embodiment and test, the invention will be further described.
Embodiment 1
A kind of extracting method of Hypoxylon fermentation broth extract, includes the following steps:
(1) culture of Hypoxylon: Hypoxylon is placed in culture medium, and 5d is cultivated at 28 DEG C and obtains culture solution, the training
Supporting based formulas is potato 2g, soybean protein 5g, pinus sylvestris var. mongolica fiber 6g, glucose 12g, KH2PO4、MgSO4, water 100ml；
(2) alcohol extracting: using gauze after culture solution filtration sterilization, will use mass ratio for the ethyl alcohol of 10:5:2, propyl alcohol,
Primary extract is obtained by extraction in ethylene glycol mixture, and primary extract is placed in supercritical extract axe and carries out supercritical extract,
40min, which is extracted, as entrainer using dehydrated alcohol obtains the Hypoxylon fermentation broth extract；The supercritical carbon dioxide extracting
Temperature is 40 DEG C, extracting pressure 70kpa, carbon dioxide flow rate 25L/min.
Embodiment 2
A kind of extracting method of Hypoxylon fermentation broth extract, includes the following steps:
(1) culture of Hypoxylon: Hypoxylon is placed in culture medium, and 6d is cultivated at 25 DEG C and obtains culture solution, the training
Supporting based formulas is potato 2g, soybean protein 5g, pinus sylvestris var. mongolica fiber 6g, glucose 12g, KH2PO4、MgSO4, water 100ml；
(2) alcohol extracting: using gauze after culture solution filtration sterilization, will use mass ratio for the ethyl alcohol of 10:5:2, propyl alcohol,
Primary extract is obtained by extraction in ethylene glycol mixture, and primary extract is placed in supercritical extract axe and carries out supercritical extract,
30min, which is extracted, as entrainer using dehydrated alcohol obtains the Hypoxylon fermentation broth extract；The supercritical carbon dioxide extracting
Temperature is 60 DEG C, extracting pressure 70kpa, carbon dioxide flow rate 25L/min.
Embodiment 3
A kind of extracting method of Hypoxylon fermentation broth extract, includes the following steps:
(1) culture of Hypoxylon: Hypoxylon is placed in culture medium, and 6d is cultivated at 26 DEG C and obtains culture solution, the training
Supporting based formulas is potato 2g, soybean protein 5g, pinus sylvestris var. mongolica fiber 6g, glucose 12g, KH2PO4、MgSO4, water 100ml；
(2) alcohol extracting: using gauze after culture solution filtration sterilization, will use mass ratio for the ethyl alcohol of 10:5:2, propyl alcohol,
Primary extract is obtained by extraction in ethylene glycol mixture, and primary extract is placed in supercritical extract axe and carries out supercritical extract,
35min, which is extracted, as entrainer using dehydrated alcohol obtains the Hypoxylon fermentation broth extract；The supercritical carbon dioxide extracting
Temperature is 50 DEG C, extracting pressure 70kpa, carbon dioxide flow rate 25L/min.
Application examples 1
Embodiment 1 is extracted to obtained Hypoxylon fermentation broth extract application mango disease control, specific application method
Are as follows: reagent is made in Hypoxylon fermentation broth extract and is periodically sprinkled in mango；The reagent contains the following component of mass parts
Composition: 100 parts of Hypoxylon fermentation broth extract, 1 part of Kocide SD, 5 parts of glucose, 1 part of hydrogen peroxide, nonionic surfactant
6 parts, 4 parts of cationic surfactant, 0.3 part of titanium dioxide, 1 part of lauric acid.The nonionic surfactant is dehydration
Sorbitol ester and sucrose ester, the cationic surfactant are quaternary cationics.
The reagent is prepared by the following method: by Hypoxylon fermentation broth extract and anionic surfactant, sun
Ion surface active, after agent mixing using add after ultrasonic disperse 20min titanium dioxide, lauric acid, Kocide SD, glucose,
Hydrogen peroxide, then using mixing 80min under ultrasonic disperse and churned mechanically collective effect up to the reagent.
Application examples 2
Embodiment 2 is extracted to obtained Hypoxylon fermentation broth extract application mango disease control, specific application method
Are as follows: reagent is made in Hypoxylon fermentation broth extract and is periodically sprinkled in mango；The reagent contains the following component of mass parts
Composition: 80 parts of Hypoxylon fermentation broth extract, 3 parts of Kocide SD, 2 parts of glucose, 6 parts of hydrogen peroxide, nonionic surfactant 2
Part, 8 parts of cationic surfactant, 0.1 part of titanium dioxide, 3 parts of lauric acid.The nonionic surfactant sucrose ester,
The cationic surfactant is quaternary cationics.
The reagent is prepared by the following method: by Hypoxylon fermentation broth extract and anionic surfactant, sun
Ion surface active, after agent mixing using add after ultrasonic disperse 10min titanium dioxide, lauric acid, Kocide SD, glucose,
Hydrogen peroxide, then using mixing 90min under ultrasonic disperse and churned mechanically collective effect up to the reagent.
Application examples 3
Embodiment 3 is extracted to obtained Hypoxylon fermentation broth extract application mango disease control, specific application method
Are as follows: reagent is made in Hypoxylon fermentation broth extract and is periodically sprinkled in mango；The reagent contains the following component of mass parts
Composition: 90 parts of Hypoxylon fermentation broth extract, 2 parts of Kocide SD, 3 parts of glucose, 5 parts of hydrogen peroxide, nonionic surfactant 5
Part, 7 parts of cationic surfactant, 0.2 part of titanium dioxide, 2 parts of lauric acid.The nonionic surfactant is dehydration mountain
Pears alcohol ester, the cationic surfactant are quaternary cationics.
The reagent is prepared by the following method: by Hypoxylon fermentation broth extract and anionic surfactant, sun
Ion surface active, after agent mixing using add after ultrasonic disperse 15min titanium dioxide, lauric acid, Kocide SD, glucose,
Hydrogen peroxide, then using mixing 85min under ultrasonic disperse and churned mechanically collective effect up to the reagent.
The Hypoxylon fermentation broth extract obtained in order to illustrate extraction of the invention is imitated in the technology of mango crop protection
Following comparative example is arranged in fruit.
Comparative example 1
Comparative example 1 it is essentially identical with the application method of application examples 1, difference is, in the reagent of comparative example 1 do not contain two
Titanium oxide.
Comparative example 2
Comparative example 2 it is essentially identical with the application method of application examples 1, difference is, in the reagent of comparative example 2 do not contain hydrogen
Copper oxide and hydrogen peroxide.
Comparative example 3
Comparative example 3 it is essentially identical with the application method of application examples 1, difference is, in the reagent of comparative example 3 do not contain the moon
Cinnamic acid and glucose.
Comparative example 4
Comparative example 4 it is essentially identical with the application method of application examples 1, difference is, the charcoal contained in the reagent of comparative example 4
In the extracting method of group's fermented liquid extract, step (2) alcohol extracting: using gauze by after culture solution filtration sterilization, using nothing
Primary extract is obtained by extraction in water-ethanol, and primary extract is placed in supercritical extract axe and carries out supercritical extract, with anhydrous
Ethyl alcohol is that entrainer extraction 40min obtains the Hypoxylon fermentation broth extract；The supercritical carbon dioxide extracting temperature is
40 DEG C, extracting pressure 70kpa, carbon dioxide flow rate 25L/min.
Comparative example 5
Comparative example 5 it is essentially identical with the application method of application examples 1, difference is, the charcoal contained in the reagent of comparative example 5
In the extracting method of group's fermented liquid extract, step (2) alcohol extracting: using gauze by after culture solution filtration sterilization, using chlorine
It is imitative that primary extract is obtained by extraction, primary extract is placed in supercritical extract axe and carries out supercritical extract, with dehydrated alcohol
30min, which is extracted, for entrainer obtains the Hypoxylon fermentation broth extract；The supercritical carbon dioxide extracting temperature is 60 DEG C,
Extracting pressure is 70kpa, carbon dioxide flow rate 25L/min.
It is following to test in order to illustrate technical effect of the invention；
According to the application method that 1~embodiment of embodiment 3 and 1~comparative example of comparative example 5 provide, by Hypoxylon fermentation liquid
Extract is applied in mango disease control, and every month sprays the reagent once containing Hypoxylon fermentation broth extract, plants
The disease incidence that mango anthracnose, gummosis branch-rot, bacterial black rot, mango graywall are counted in journey, is recorded in table 1.
Table 1
As shown in Table 1, embodiment 1 is compared to the mango anthracnose of 1~comparative example of comparative example 3, gummosis branch-rot, bacillary
Black spot, mango graywall illness rate are all low, illustrate the present invention using Hypoxylon fermentation broth extract, Kocide SD, glucose,
Hydrogen peroxide, nonionic surfactant, cationic surfactant, titanium dioxide, the reagent of lauric acid mutual cooperation composition are anti-
Controlling mango anthracnose, gummosis branch-rot, bacterial black rot, mango graywall has good effect, and embodiment 1 is compared to right
Ratio 4, the mango anthracnose of comparative example 5, gummosis branch-rot, bacterial black rot, mango graywall illness rate are low, the charcoal of explanation
Group's fermented liquid extract method can be extracted to obtain effective sterilization ingredient living, the Hypoxylon obtained compared to other extracting methods
Fermentation broth extract sterilizing disease-preventing effect is good.
The mango band tail noctuid of 1~application examples of application examples 3, Mango Leaf-hopper, dacus dorsalis hendel of mandarin orange, mango ridge chest day
Ox, mango cecidomyiia cut leaf weevil, and the toxic effect of aphid and scale insect is respectively as follows: the mango band tail noctuid of poisoning 58%, poison
69% Mango Leaf-hopper, the dacus dorsalis hendel of mandarin orange of poisoning 46%, the mango ridge chest longicorn for killing 23% are killed, 41% awns is killed
Fruit cecidomyiia kills 58% and cuts leaf weevil, kills 100% aphid and scale insect.
Above description is the detailed description for the present invention preferably possible embodiments, but embodiment is not limited to this hair
Bright patent claim, it is all the present invention suggested by technical spirit under completed same changes or modifications change, should all belong to
In the covered the scope of the patents of the present invention.
Claims (6)
1. a kind of extracting method of Hypoxylon fermentation broth extract, which comprises the steps of:
(1) culture of Hypoxylon: Hypoxylon is placed in culture medium, and 5~6d is cultivated at 25~28 DEG C and obtains culture solution, described
Culture medium prescription is potato 2g, soybean protein 5g, pinus sylvestris var. mongolica fiber 6g, glucose 12g, KH2PO4、MgSO4, water 100ml；
(2) alcohol extracting: using gauze by after culture solution filtration sterilization, use mass ratio for the ethyl alcohol of 10:5:2, propyl alcohol, second two
Primary extract is obtained by extraction in alcohol mixed liquor, and primary extract is placed in supercritical extract axe and carries out supercritical extract, with nothing
Water-ethanol is that 30~40min of entrainer extraction obtains the Hypoxylon fermentation broth extract；The supercritical carbon dioxide extracting
Temperature is 40~60 DEG C, extracting pressure 70kpa, carbon dioxide flow rate 25L/min.
2. a kind of application of Hypoxylon fermentation broth extract described in claim 1 in mango disease control, which is characterized in that
The Hypoxylon fermentation broth extract is prepared into reagent and periodically sprinkled in mango by it；The reagent contains the as follows of mass parts
Group is grouped as: 80~100 parts of Hypoxylon fermentation broth extract, 1~3 part of Kocide SD, 2~5 parts of glucose, hydrogen peroxide 1~6
Part, 2~6 parts of nonionic surfactant, 4~8 parts of cationic surfactant, 0.1~0.3 part of titanium dioxide, lauric acid 1~
3 parts.
3. application of the Hypoxylon fermentation broth extract according to claim 2 in mango disease control, which is characterized in that
The nonionic surfactant is polyol type.
4. application of the Hypoxylon fermentation broth extract according to claim 3 in mango disease control, which is characterized in that
The nonionic surfactant is the one or two of sorbitan ester, sucrose ester.
5. application of the Hypoxylon fermentation broth extract according to claim 2 in mango disease control, which is characterized in that
The cationic surfactant is quaternary cationics.
6. application of the Hypoxylon fermentation broth extract according to claim 2 in mango disease control, which is characterized in that
The reagent is prepared by the following method: by Hypoxylon fermentation broth extract and anionic surfactant, cationic surface
Using addition titanium dioxide, lauric acid, Kocide SD, glucose, dioxygen after 10~20min of ultrasonic disperse after activating agent mixing
Water, then using 80~90min of mixing under ultrasonic disperse and churned mechanically collective effect up to the reagent.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810888964.2A CN108991020A (en) | 2018-08-07 | 2018-08-07 | A kind of extracting method of Hypoxylon fermentation broth extract and its application in mango disease control |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810888964.2A CN108991020A (en) | 2018-08-07 | 2018-08-07 | A kind of extracting method of Hypoxylon fermentation broth extract and its application in mango disease control |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108991020A true CN108991020A (en) | 2018-12-14 |
Family
ID=64595974
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810888964.2A Pending CN108991020A (en) | 2018-08-07 | 2018-08-07 | A kind of extracting method of Hypoxylon fermentation broth extract and its application in mango disease control |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108991020A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109536390A (en) * | 2018-12-21 | 2019-03-29 | 浙江省柑桔研究所 | A kind of citrus endogenetic fungus and its application |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101897345A (en) * | 2009-06-01 | 2010-12-01 | 东北林业大学 | Preparation method of preparation containing hypoxylon bacteriostatic active ingredients for preventing and treating sphaeropsis sapinea |
| CN106755118A (en) * | 2016-11-18 | 2017-05-31 | 广西壮族自治区药用植物园 | The preparation method of dark-coloured ring grain Hypoxylon active metabolite |
| CN108077258A (en) * | 2017-12-28 | 2018-05-29 | 四川省农业科学院生物技术核技术研究所 | A kind of granule of biocontrol of mango bacterial disease and preparation method thereof |
-
2018
- 2018-08-07 CN CN201810888964.2A patent/CN108991020A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101897345A (en) * | 2009-06-01 | 2010-12-01 | 东北林业大学 | Preparation method of preparation containing hypoxylon bacteriostatic active ingredients for preventing and treating sphaeropsis sapinea |
| CN106755118A (en) * | 2016-11-18 | 2017-05-31 | 广西壮族自治区药用植物园 | The preparation method of dark-coloured ring grain Hypoxylon active metabolite |
| CN108077258A (en) * | 2017-12-28 | 2018-05-29 | 四川省农业科学院生物技术核技术研究所 | A kind of granule of biocontrol of mango bacterial disease and preparation method thereof |
Non-Patent Citations (3)
| Title |
|---|
| 潘春梅: "《微生态制剂生产及应用》", 30 September 2014, 中国农业大学出版社 * |
| 王运兵: "《无公害农药实用手册》", 31 March 2004, 河南科学技术出版社 * |
| 高海燕: "炭团菌抑菌活性成分及其对松枯梢病菌的影响", 《东北林业大学硕士论文》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109536390A (en) * | 2018-12-21 | 2019-03-29 | 浙江省柑桔研究所 | A kind of citrus endogenetic fungus and its application |
| CN109536390B (en) * | 2018-12-21 | 2022-04-15 | 浙江省柑桔研究所 | Citrus endophytic fungi and application thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104756869B (en) | The preparation of masson pine fine individual plant aseptic explant and initial bud inducement method | |
| CN105875210A (en) | Method for grafting bergamots | |
| CN107548928A (en) | A kind of implantation methods of greenhouse organic spinach | |
| CN106577300B (en) | The method for improving squalene content in Siraitia grosvenorii | |
| CN105923711B (en) | A kind of functional water and its functional water dimension stereo agriculture planting method | |
| CN103408335B (en) | Microbiological method based harmlessness treatment technology for agricultural wastes generated by grape cultivation | |
| CN117256395A (en) | Cultivation method of endangered plant garlic fruit seedlings | |
| CN103404536A (en) | Compound biological herbicide and application method thereof | |
| Campayo et al. | Novel endotherapy-based applications of ozonated water to Bobal grapevines: effect on grape quality | |
| KR102144072B1 (en) | Method for preparing nutrient solution for ginseng water culture | |
| CN107306720A (en) | A kind of cultural method of high yield citrus | |
| CN113912461A (en) | Foliar fertilizer for resisting plant leaf fungal diseases | |
| CN108991020A (en) | A kind of extracting method of Hypoxylon fermentation broth extract and its application in mango disease control | |
| JP2005533118A (en) | Environmentally safe agricultural supplements | |
| CN109232107A (en) | Yeast selenium foliar fertilizer, production method and application | |
| CN107318580A (en) | A kind of implantation methods of grape | |
| CN105831162A (en) | Sprouting vegetable seed soaking fungicide and application thereof | |
| CN102204575A (en) | Silktree-albizia-extract-based preparation for controlling tetranychus cinnabarinus, and preparation and application thereof | |
| KR101409081B1 (en) | Peach chronic treatment solution using natural ingredients and method of manufacturing the same | |
| Rana | Influence of Panchgavya on growth, yield and quality parameters of bitter gourd (Momordica charantia L.) | |
| CN106954640A (en) | A kind of method of utilization microbial control trees disease | |
| CN109729911A (en) | A kind of acclimation method of wild selenium-rich mushroom strain | |
| Conant | CASE 6. Pasteur’s Study of Fermentation | |
| US20160066581A1 (en) | Silver ion solution agricultural formulations | |
| CN109121766A (en) | A kind of implantation methods improving health-care granada value |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20181214 |
|
| RJ01 | Rejection of invention patent application after publication |