CN108976192A - A kind of eucalyptus leaves anthocyanidin preparation method - Google Patents
A kind of eucalyptus leaves anthocyanidin preparation method Download PDFInfo
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D311/00—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings
- C07D311/02—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings ortho- or peri-condensed with carbocyclic rings or ring systems
- C07D311/04—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring
- C07D311/58—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring other than with oxygen or sulphur atoms in position 2 or 4
- C07D311/60—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring other than with oxygen or sulphur atoms in position 2 or 4 with aryl radicals attached in position 2
- C07D311/62—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring other than with oxygen or sulphur atoms in position 2 or 4 with aryl radicals attached in position 2 with oxygen atoms directly attached in position 3, e.g. anthocyanidins
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Abstract
The present invention relates to anthocyanidin processing technique fields, in particular to a kind of eucalyptus leaves anthocyanidin preparation method, it includes that eucalyptus leaves pre-process that the eucalyptus leaves anthocyanidin of the application, which extracts, blue light processing, anthocyanidin extract and cyanine it is plain mention four steps be made, in preprocessing process, it is handled by organic acid soln, it is stifling, cellulase treatment, the active material in eucalyptus blade can be dissolved out well, blue light processing can promote enzymatic reaction to carry out, it can be further improved the content of anthocyanidin in extract after organic solvent extracts and macroreticular resin proposes only, tannin content in extract can also be effectively reduced simultaneously.
Description
[technical field]
The present invention relates to anthocyanidin processing technique field, in particular to a kind of eucalyptus leaves anthocyanidin preparation method.
[background technique]
Eucalyptus (eucalyptus) is a kind of activity fast-growing class plant abundant, and root, stem, leaf all have medical value,
In eucalyptus leaves contain a large amount of active constituents, main component 1,8- cineole have it is good fat-soluble, readily penetrate through biology
Membrane structure, sharing with other drugs has the function of that promotion is transdermal.Other ingredients in folium eucalypti, as terpene, flavonoids, tannin,
Phloroglucinol derivatives and glycoside etc. also have bioactivity abundant, these ingredients have been widely used in fungicide effect,
However, also containing a small amount of anthocyanidin in eucalyptus leaves, since its content is very low, it is quite big to extract difficulty.If a kind of eucalyptus can be found out
The extracting method of tree anthocyanidin will greatly enhance the added value of product of eucalyptus, extend its machining chain.
[summary of the invention]
In view of above content, it is necessary to provide the preparation method of a kind of the anthocyanidin yield for improving eucalyptus leaves and recovery rate.
In order to achieve the above objectives, the technical scheme adopted by the invention is that:
A kind of eucalyptus leaves anthocyanidin preparation method, described method includes following steps:
(1) eucalyptus leaves pre-process: eucalyptus leaves being mixed with pretreatment fluid according to mass ratio for 1:1-3, are then placed in
- 26h for 24 hours is heated in the water-bath that temperature is 40 DEG C -50 DEG C, filtrate is taken to obtain eucalyptus leaves leachable after filtering；Eucalyptus leaves are carried out
Stifling 40min-50min, then mixes with biological enzyme solutions, and constant temperature handles 12h-14h in 36 DEG C -38 DEG C of insulating box；So
The centrifugal treating 5min-15min in the centrifuge that revolving speed is 1000r/min-1500r/min afterwards, takes supernatant to complete eucalyptus leaves
Pretreatment；
(2) blue light is handled: the eucalyptus leaves clear liquid that step (1) has pre-processed is put into blue light lamp box and carries out blue light illumination,
A length of 48h-50h when irradiation；The blue light intensity of illumination of irradiation is 1000Lx-1500Lx；
(3) anthocyanidin extract: by after step (2) blue light illumination eucalyptus leaves clear liquid and organic solvent according to mass ratio be 1:
3-5 is mixed, and is then placed in supersonic extractors and is carried out ultrasonic extraction；Rotation is concentrated by evaporation, dries after extracting 12h-14h
Obtain anthocyanidin coarse powder；
(4) cyanine is plain mentions: the anthocyanidin coarse powder of step (3) is mixed with distilled water according to mass ratio for 1:10-15
To anthocyanidin solution, then adsorbed with macroporous resin column；When absorption, column flow rate is 0.5-1 times of cylinder on anthocyanidin solution
Product/hour, upper column temperature are 30-40 DEG C, are eluted after the completion of absorption with the ethanol solution that percentage by volume is 75%, so
After be concentrated in vacuo, be freeze-dried to obtain the eucalyptus leaves anthocyanidin；The upper column flow rate of ethanol solution is 1- when the elution
1.5 times of column volume/hours.
Further, the pretreatment fluid of the step (1) by tartaric acid solution, citric acid solution and malic acid solution according to
Mass ratio is 1:1-3:2-4 composition；The mass concentration of tartaric acid solution mesotartaric acid is 3-7mg/L；Lemon in citric acid solution
The mass concentration of acid is 10-15mg/L；The mass concentration of malic acid is 5-15mg/L in malic acid solution.
Further, the stifling temperature of the step (1) is 105 DEG C -110 DEG C.
Further, step (1) biological enzyme is 1:3-5:4- according to mass ratio by protease, tannase and lipase
6 are mixed to prepare；The enzyme activity of the protease is 800u/g-1000u/g, and the enzyme activity of tannase is 800u/g-1000u/g,
The enzyme activity of lipase is 1000u/g-1200u/g.
Further, step (3) organic solvent is 5-8:2-4:1 according to mass ratio by ethyl alcohol, methanol and n-butanol
Composition；The percentage by volume of the ethyl alcohol is 95%；The percentage by volume of the methanol is 25%；The volume hundred of the n-butanol
Score is 15%.
Further, the extraction power of step (3) ultrasonic extraction is 500w-1000w.
The invention has the following beneficial effects:
It includes eucalyptus leaves pretreatment that the eucalyptus leaves anthocyanidin of the application, which extracts, blue light processing, anthocyanidin extracts and anthocyanidin
Only four steps are proposed, in preprocessing process, pretreatment fluid is organic by tartaric acid solution, citric acid solution and malic acid solution etc.
Acid composition, these Determination of Organic Acids can corrode eucalyptus leaves in acidity well, anti-with the active material progress in blade after heating
It answers, eucalyptus vein can be effectively removed after filtering (ingredient is mainly lignin)；Acid processing heating after, in filtrate can containing it is higher at
The tannin and essential oil substance divided；Tannin and essential oil will be effectively removed after stifling, still containing richer in reaction solution at this time
The macromolecular substances, especially colloid such as rich colloid, cellulose can form coating to influence the precipitation of anthocyanidin；Application
Addition biological enzyme will promote the decomposition of macromolecular substances to people's discovery at this time, broken wall be carried out for the cell of eucalyptus leaves, in quickening
The precipitation of molten object, to improve anthocyanidin yield；It is found in applicant's research process, if carrying out blue light to extracting solution at this time
Processing will further be enriched with anthocyanidin, and concrete reason is also making further research at present, tentatively conclude because blue light promotes
Certain enzymatic reactions promote the synthesis of anthocyanidin intermediates；After blue light illumination, is extracted by organic solvent and macroreticular resin is inhaled
It is attached, it can effectively promote the precipitation and purifying of anthocyanidin, can be effectively improved and be mentioned using the present processes production eucalyptus leaves anthocyanidin
The content and yield of anthocyanidin in object are taken, while tannin content in extract can also be effectively reduced.
[specific embodiment]
All features disclosed in this specification or disclosed all methods or in the process the step of, in addition to mutually exclusive
Feature and/or step other than, can combine in any way.
Any feature disclosed in this specification (including any accessory claim, abstract), unless specifically stated, each
Feature is an example in a series of equivalent or similar characteristics.
Embodiment 1:
A kind of eucalyptus leaves anthocyanidin preparation method is present embodiments provided, this method comprises the following steps:
(1) eucalyptus leaves pre-process: by eucalyptus leaves and pretreatment fluid according to mass ratio be 1:1 mixed (pretreatment fluid by
The apple that the citric acid solution and mass concentration that tartaric acid solution that mass concentration is 3mg/L, mass concentration are 10mg/L are 5mg/L
Tartaric acid solution is mixed to prepare according to mass ratio for 1:1:2), it is then placed in the water-bath that temperature is 40 DEG C and heats for 24 hours, taken after filtering
Filtrate obtains eucalyptus leaves leachable；By eucalyptus leaves under conditions of temperature is 105 DEG C stifling 40min, then with biological enzyme solutions
(the tannic acid enzyme solutions and enzyme activity that protein enzyme solution that biological enzyme is 800u/g by enzyme activity, enzyme activity are 800u/g are for mixing
The lipase solution of 1000u/g is mixed to prepare according to mass ratio for 1:3:4), constant temperature handles 12h in 36 DEG C of insulating box；So
The centrifugal treating 5min in the centrifuge that revolving speed is 1000r/min afterwards takes supernatant to complete eucalyptus leaves pretreatment；
(2) blue light is handled: the eucalyptus leaves clear liquid that step (1) has pre-processed is put into blue light lamp box and carries out blue light illumination,
A length of 48h when irradiation；The blue light intensity of illumination of irradiation is 1000Lx；
(3) anthocyanidin extract: by after step (2) blue light illumination eucalyptus leaves clear liquid and organic solvent according to mass ratio be 1:
3 mixed the (methanol and volume basis that ethyl alcohol that organic solvent is 95% by percentage by volume, percentage by volume are 25%
The n-butanol that number is 15% is that 5:2:1 is mixed to prepare according to mass ratio)；It is then placed in supersonic extractors and carries out ultrasonic extraction,
The power of ultrasonic extraction is 500w；Rotation is concentrated by evaporation after extraction 12h, drying obtains anthocyanidin coarse powder；
(4) cyanine is plain mentions: the anthocyanidin coarse powder of step (3) and distilled water are mixed to get flower according to mass ratio for 1:10
Green element solution, is then adsorbed with macroporous resin column；When absorption, column flow rate is 0.5 times of column volume/small on anthocyanidin solution
When, upper column temperature is 30 DEG C, is eluted after the completion of absorption with the ethanol solution that percentage by volume is 75%, then carries out vacuum
Concentration, freeze-drying obtain the eucalyptus leaves anthocyanidin；The upper column flow rate of ethanol solution is 1 times of column volume/small when the elution
When.
Embodiment 2:
A kind of eucalyptus leaves anthocyanidin preparation method is present embodiments provided, this method comprises the following steps:
(1) eucalyptus leaves pre-process: by eucalyptus leaves and pretreatment fluid according to mass ratio be 1:3 mixed (pretreatment fluid by
The citric acid solution and mass concentration that tartaric acid solution that mass concentration is 7mg/L, mass concentration are 10-15mg/L are 15mg/L
Malic acid solution be mixed to prepare according to mass ratio for 1:3:4), being then placed in temperature is to heat 26h in 50 DEG C of water-bath, is filtered
After take filtrate to obtain eucalyptus leaves leachable；By eucalyptus leaves under conditions of temperature is 110 DEG C stifling 50min, then with biological enzyme
Solution mixing (the tannic acid enzyme solutions and enzyme that protein enzyme solution that biological enzyme is 1000u/g by enzyme activity, enzyme activity are 1000u/g
The lipase solution that vigor is 1200u/g is that 1:5:6 is mixed to prepare according to mass ratio), constant temperature is handled in 38 DEG C of insulating box
14h；Then the centrifugal treating 15min in the centrifuge that revolving speed is 1500r/min takes supernatant to complete eucalyptus leaves pretreatment；
(2) blue light is handled: the eucalyptus leaves clear liquid that step (1) has pre-processed is put into blue light lamp box and carries out blue light illumination,
A length of 50h when irradiation；The blue light intensity of illumination of irradiation is 1500Lx；
(3) anthocyanidin extract: by after step (2) blue light illumination eucalyptus leaves clear liquid and organic solvent according to mass ratio be 1:
5 mixed the (methanol and volume basis that ethyl alcohol that organic solvent is 95% by percentage by volume, percentage by volume are 25%
The n-butanol that number is 15% is that 8:4:1 is mixed to prepare according to mass ratio)；It is then placed in supersonic extractors and carries out ultrasonic extraction,
The power of ultrasonic extraction is 1000w；Rotation is concentrated by evaporation after extraction 14h, drying obtains anthocyanidin coarse powder；
(4) cyanine is plain mentions: the anthocyanidin coarse powder of step (3) and distilled water are mixed to get flower according to mass ratio for 1:15
Green element solution, is then adsorbed with macroporous resin column；When absorption, column flow rate is 1 times of column volume/hour on anthocyanidin solution,
Upper column temperature is 40 DEG C, is eluted after the completion of absorption with the ethanol solution that percentage by volume is 75%, and it is dense then to carry out vacuum
Contracting, freeze-drying obtain the eucalyptus leaves anthocyanidin；The upper column flow rate of ethanol solution is 1.5 times of column volumes/small when the elution
When.
Embodiment 3:
A kind of eucalyptus leaves anthocyanidin preparation method is present embodiments provided, this method comprises the following steps:
(1) eucalyptus leaves pre-process: by eucalyptus leaves and pretreatment fluid according to mass ratio be 1:2 mixed (pretreatment fluid by
The apple that the citric acid solution and mass concentration that tartaric acid solution that mass concentration is 5mg/L, mass concentration are 12mg/L are 7mg/L
Tartaric acid solution is mixed to prepare according to mass ratio for 1:2:3), it is then placed in the water-bath that temperature is 45 DEG C and heats 25h, taken after filtering
Filtrate obtains eucalyptus leaves leachable；By eucalyptus leaves under conditions of temperature is 108 DEG C stifling 45min, then with biological enzyme solutions
(the tannic acid enzyme solutions and enzyme activity that protein enzyme solution that biological enzyme is 900u/g by enzyme activity, enzyme activity are 900u/g are for mixing
The lipase solution of 1100u/g is mixed to prepare according to mass ratio for 1:2:5), constant temperature handles 12h- in 37 DEG C of insulating box
14h；Then the centrifugal treating 8min in the centrifuge that revolving speed is 1200r/min takes supernatant to complete eucalyptus leaves pretreatment；
(2) blue light is handled: the eucalyptus leaves clear liquid that step (1) has pre-processed is put into blue light lamp box and carries out blue light illumination,
A length of 49h when irradiation；The blue light intensity of illumination of irradiation is 1300Lx；
(3) anthocyanidin extract: by after step (2) blue light illumination eucalyptus leaves clear liquid and organic solvent according to mass ratio be 1:
4 mixed the (methanol and volume basis that ethyl alcohol that organic solvent is 95% by percentage by volume, percentage by volume are 25%
The n-butanol that number is 15% is that 6:3:1 is mixed to prepare according to mass ratio)；It is then placed in supersonic extractors and carries out ultrasonic extraction,
The power of ultrasonic extraction is 700w；Rotation is concentrated by evaporation after extraction 13h, drying obtains anthocyanidin coarse powder；
(4) cyanine is plain mentions: the anthocyanidin coarse powder of step (3) and distilled water are mixed to get flower according to mass ratio for 1:12
Green element solution, is then adsorbed with macroporous resin column；When absorption, column flow rate is 0.8 times of column volume/small on anthocyanidin solution
When, upper column temperature is 35 DEG C, is eluted after the completion of absorption with the ethanol solution that percentage by volume is 75%, then carries out vacuum
Concentration, freeze-drying obtain the eucalyptus leaves anthocyanidin；When the elution the upper column flow rate of ethanol solution be 1.3 times of column volumes/
Hour.
Control group 1:
This control group does not have to pretreatment fluid and handles eucalyptus leaves, and other preparation methods are identical with embodiment 1.
Control group 2:
This control group does not carry out suffocating treatment to eucalyptus leaves, and other preparation methods are identical with embodiment 1.
Control group 3:
This control group is handled eucalyptus leaves without using biological enzyme, and other preparation methods are identical with embodiment 1.
Control group 4:
This control group is handled without using blue light, and other preparation methods are identical with embodiment 1.
Control group 5:
Ethyl alcohol is used only in this control group organic solvent, that is, in the extraction of step (3) anthocyanidin: by step (2) blue light illumination
The ethanol solution that eucalyptus leaves clear liquid afterwards and percentage by volume are 95% is that 1:3 is mixed according to mass ratio, other preparation sides
Method is identical with embodiment 1.
Yield and purity test:
Anthocyanidin, tannin content in embodiment 1-3 and control group 1-5 extract are counted and detected, and calculates anthocyanidin and obtains
Rate, yield are calculated by following formula and are obtained, and concrete condition is shown in Table 1:
Table 1
As seen from the above table, the anthocyanidin content of embodiment 1-3, yield are all apparently higher than control group 1-5, illustrate the application's
Pretreatment fluid processing, suffocating treatment, cellulase treatment, blue light illumination processing and organic solvent formula can improve this well
The eucalyptus leaves anthocyanidin content of application, and it is indispensable；The tannin content of embodiment 1-3 is significantly lower than control group 1-3, and compares
Group 4-5 is not much different, and illustrates that the pretreatment fluid processing of the application, suffocating treatment, cellulase treatment can be effectively reduced in extract
Tannin content, and it is indispensable.
Influence test of the pretreatment fluid component to tannin content:
Eucalyptus leaves are pre-processed according to following processing group, test pretreatment front and back eucalyptus leaves tannin content, processing group
It is as follows:
Processing 1: eucalyptus leaves are pre-processed according to the method for the embodiment of the present application 1；
Processing 2: tartaric acid solution is free of in pretreatment fluid, other processing modes are identical with embodiment 1, that is, pre-process
The malic acid solution that the citric acid solution and mass concentration that liquid is 10mg/L by mass concentration are 5mg/L is 1:2 according to mass ratio
It is mixed to prepare.
Processing 3: citric acid solution is free of in pretreatment fluid, other processing modes are identical with embodiment 1, that is, pre-process
The malic acid solution that the tartaric acid solution and mass concentration that liquid is 3mg/L by mass concentration are 5mg/L is that 1:2 is mixed according to mass ratio
It closes and is made.
Processing 4: tartaric acid solution is free of in pretreatment fluid, other processing modes are identical with embodiment 1, that is, pre-process
The citric acid solution that the tartaric acid solution and mass concentration that liquid is 3mg/L by mass concentration are 10mg/L is 1:1 according to mass ratio
It is mixed to prepare.
Test result is shown in Table 2:
Table 2
| Tannin content (mg/g) | Processing 1 | Processing 2 | Processing 3 | Processing 4 |
| Before processing | 24.35 | 25.13 | 24.08 | 25.19 |
| After processing | 4.03 | 12.31 | 13.26 | 15.33 |
As seen from the above table, the ability of 1 processing tannin of processing is most strong, and the ability of processing 2-4 processing tannin is not so good as processing 1, by
This illustrates that content, the component of organic acid can reduce tannin content well in the application pretreatment fluid component, and ingredient lacks one not
It can.
Influence test of the biological enzyme solution component to tannin content:
Eucalyptus leaves are pre-processed according to following processing group, test pretreatment front and back eucalyptus leaves tannin content, processing group
It is as follows:
Processing A: eucalyptus leaves are pre-processed according to the method for the embodiment of the present application 1；
It handles B: being free of protein enzyme solution in pretreatment fluid, other processing modes are identical with embodiment 1, i.e. biological enzyme
The lipase solution that the tannic acid enzyme solutions and enzyme activity that liquid is 800u/g by enzyme activity are 1000u/g is that 3:4 is mixed according to mass ratio
It closes and is made.
It handles C: being free of tannic acid enzyme solutions in pretreatment fluid, other processing modes are identical with embodiment 1, i.e. biological enzyme
The lipase solution that the protein enzyme solution and enzyme activity that liquid is 800u/g by enzyme activity by biological enzyme are 1000u/g is according to mass ratio
It is mixed to prepare for 1:4.
It handles D: being free of lipase solution in pretreatment fluid, other processing modes are identical with embodiment 1, i.e. biological enzyme
The tannic acid enzyme solutions that the protein enzyme solution and enzyme activity that liquid is 800u/g by enzyme activity by biological enzyme are 800u/g are according to mass ratio
It is mixed to prepare for 1:3.
Test result is shown in Table 3:
Table 3
| Tannin content (mg/g) | Handle A | Handle B | Handle C | Handle D |
| Before processing | 24.35 | 25.13 | 24.08 | 25.19 |
| After processing | 4.03 | 8.65 | 9.06 | 8.33 |
As seen from the above table, the ability of processing A processing tannin is most strong, and the ability of processing B-D processing tannin is not so good as processing A, by
This illustrates that content, the component of biological enzyme can reduce tannin content well in the application biology enzyme solution component, and ingredient lacks one not
It can.
In conclusion containing for anthocyanidin in extract can be effectively improved using method production eucalyptus leaves anthocyanidin of the invention
Amount and yield, while tannin content in extract can also be effectively reduced.
The embodiments described above only express several embodiments of the present invention, and the description thereof is more specific and detailed, but simultaneously
Limitation of the scope of the invention therefore cannot be interpreted as.It should be pointed out that for those of ordinary skill in the art,
Without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to protection model of the invention
It encloses.Therefore, protection scope of the present invention should be determined by the appended claims.
Claims (6)
1. a kind of eucalyptus leaves anthocyanidin preparation method, which is characterized in that described method includes following steps:
(1) eucalyptus leaves pre-process: eucalyptus leaves being mixed with pretreatment fluid according to mass ratio for 1:1-3, temperature is then placed in
To heat -26h for 24 hours in 40 DEG C -50 DEG C of water-baths, filtrate is taken to obtain eucalyptus leaves leachable after filtering；Eucalyptus leaves are fumigated
Then 40min-50min is mixed with biological enzyme solutions, constant temperature handles 12h-14h in 36 DEG C -38 DEG C of insulating box；Then exist
Revolving speed is centrifugal treating 5min-15min in the centrifuge of 1000r/min-1500r/min, takes supernatant to complete eucalyptus leaves and locates in advance
Reason；
(2) blue light is handled: the eucalyptus leaves clear liquid that step (1) has pre-processed being put into blue light lamp box and carries out blue light illumination, is irradiated
Shi Changwei 48h-50h；The blue light intensity of illumination of irradiation is 1000Lx-1500Lx；
(3) anthocyanidin extract: by after step (2) blue light illumination eucalyptus leaves clear liquid and organic solvent according to mass ratio be 1:3-5
It is mixed, is then placed in supersonic extractors and carries out ultrasonic extraction；Rotation is concentrated by evaporation after extraction 12h-14h, drying obtains
Anthocyanidin coarse powder；
(4) cyanine is plain mentions: the anthocyanidin coarse powder of step (3) and distilled water are mixed to get flower according to mass ratio for 1:10-15
Green element solution, is then adsorbed with macroporous resin column；When absorption, column flow rate is 0.5-1 times of column volume/small on anthocyanidin solution
When, upper column temperature is 30-40 DEG C, is eluted after the completion of absorption with the ethanol solution that percentage by volume is 75%, is then carried out
Vacuum concentration, freeze-drying obtain the eucalyptus leaves anthocyanidin；The upper column flow rate of ethanol solution is 1-1.5 times of column when the elution
Volume/hour.
2. eucalyptus leaves anthocyanidin preparation method according to claim 1, which is characterized in that the pretreatment of the step (1)
Liquid is made of tartaric acid solution, citric acid solution and malic acid solution according to mass ratio for 1:1-3:2-4；Wine in tartaric acid solution
The mass concentration of stone acid is 3-7mg/L；The mass concentration of citric acid is 10-15mg/L in citric acid solution；In malic acid solution
The mass concentration of malic acid is 5-15mg/L.
3. eucalyptus leaves anthocyanidin preparation method according to claim 1, which is characterized in that the stifling temperature of the step (1)
Degree is 105 DEG C -110 DEG C.
4. eucalyptus leaves anthocyanidin preparation method according to claim 1, which is characterized in that step (1) biological enzyme by
Protease, tannase and lipase are mixed to prepare according to mass ratio for 1:3-5:4-6；The enzyme activity of the protease is 800u/
G-1000u/g, the enzyme activity of tannase are 800u/g-1000u/g, and the enzyme activity of lipase is 1000u/g-1200u/g.
5. eucalyptus leaves anthocyanidin preparation method according to claim 1, which is characterized in that step (3) organic solvent
It is made of ethyl alcohol, methanol and n-butanol according to mass ratio for 5-8:2-4:1；The percentage by volume of the ethyl alcohol is 95%；It is described
The percentage by volume of methanol is 25%；The percentage by volume of the n-butanol is 15%.
6. eucalyptus leaves anthocyanidin preparation method according to claim 1, which is characterized in that step (3) ultrasonic extraction
Extraction power be 500w-1000w.
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| CN201811155488.XA CN108976192A (en) | 2018-09-30 | 2018-09-30 | A kind of eucalyptus leaves anthocyanidin preparation method |
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Application publication date: 20181211 |