CN109265426A - A kind of cowpea anthocyanidin preparation method - Google Patents
A kind of cowpea anthocyanidin preparation method Download PDFInfo
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D311/00—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings
- C07D311/02—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings ortho- or peri-condensed with carbocyclic rings or ring systems
- C07D311/04—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring
- C07D311/58—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring other than with oxygen or sulphur atoms in position 2 or 4
- C07D311/60—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring other than with oxygen or sulphur atoms in position 2 or 4 with aryl radicals attached in position 2
- C07D311/62—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring other than with oxygen or sulphur atoms in position 2 or 4 with aryl radicals attached in position 2 with oxygen atoms directly attached in position 3, e.g. anthocyanidins
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Abstract
The present invention relates to anthocyanidin processing technique fields, in particular to a kind of cowpea anthocyanidin preparation method, it includes that cowpea seed pre-processes that the cowpea anthocyanidin of the application, which extracts, blue light processing, anthocyanidin, which extracts, and cyanine is plain proposes four steps, in preprocessing process, it is extracted by vitamin C, base extraction, cellulase treatment can dissolve out the active material in cowpea seed well, blue light processing can promote enzymatic reaction to carry out, it can be further improved the content of anthocyanidin in extract after organic solvent extracts and macroreticular resin proposes only, tannin content in extract can also be effectively reduced simultaneously.
Description
[technical field]
The present invention relates to anthocyanidin processing technique field, in particular to a kind of cowpea anthocyanidin preparation method.
[background technique]
Cowpea (Vigna unguiculata) is commonly called as carob, Jiang Dou, band beans, hangs long bean.Cowpea is divided into asparagus bean and meal
Two kinds of cowpea, belong to leguminous plant.Vigna pulse family annual plant.Stem has short property, half tendril and three kinds of tendril.South cultivation is with climing
Property based on, short property is taken second place, the annual voluble herb plant of Papilionaceae, corolla be lavender, the cyanine containing higher concentration
Element, and, but the anthocyanidin content of its seed is lower, cowpea seed plant egg also rich in other than containing anthocyanidin
It is white, the industries such as feed, food can be widely used in, conventional method extracts the vegetable protein that can only extract higher concentration, and for cowpea
The extraction of beans anthocyanidin fresh understatement road, cowpea anthocyanidin has stronger bioactivity, if being able to extract and be used,
Anthocyanidin type will be further enriched, the added value of product of cowpea is improved.
[summary of the invention]
In view of above content, it is necessary to provide a kind of preparation side of anthocyanidin yield and recovery rate for improving cowpea seed
Method.
In order to achieve the above objectives, the technical scheme adopted by the invention is that:
A kind of cowpea anthocyanidin preparation method, described method includes following steps:
(1) cowpea seed pre-processes: taking the mature cowpea seed and vitamin c solution that moisture content is 2%-5% according to matter
Amount is then placed in the refrigerator-freezer that temperature is -10 DEG C~-5 DEG C than being that 1:7 is mixed and suddenly freezes 60min-80min；Room temperature after anxious jelly
It thaws, all melts to ice cube, then according to the lye of mixeding liquid volume 5%-8%, be placed on 60 after standing 20min-30min
- 26h for 24 hours is heated in DEG C -70 DEG C of water-bath, filtrate is filtered to take and obtains cowpea leachable；Its volume is added into cowpea leachable
The biological enzyme solutions of 5%-8%, isothermal reaction 3h-4h under the conditions of temperature is 36 DEG C -38 DEG C obtain cowpea seed pretreatment
Liquid；
(2) blue light is handled: the cowpea seed pretreatment fluid of step (1) being put into blue light lamp box and carries out blue light illumination, is shone
A length of 50h-52h when penetrating；The blue light intensity of illumination of irradiation is 1000Lx-1500Lx；
(3) anthocyanidin extract: by after step (2) blue light illumination cowpea seed pretreatment fluid and organic acid soln according to body
Product is than taking filtrate to be mixed with organic solvent according to mass ratio for 1:4-6, so to filter after 2-4:3-5 hybrid reaction 3h-5h
After be put into supersonic extractors and carry out ultrasonic extraction；Rotation is concentrated by evaporation after extraction 15h-17h, drying obtains anthocyanidin coarse powder；
(4) cyanine is plain mentions: the anthocyanidin coarse powder of step (3) is mixed with distilled water according to mass ratio for 1:10-15
To anthocyanidin solution, then adsorbed with macroporous resin column；When absorption, column flow rate is 0.5-1 times of cylinder on anthocyanidin solution
Product/hour, upper column temperature are 30-40 DEG C, are eluted after the completion of absorption with the ethanol solution that percentage by volume is 75%, then
It is concentrated in vacuo, is freeze-dried to obtain the cowpea anthocyanidin；The upper column flow rate of ethanol solution is 1-1.5 times when the elution
Column volume/hour.
Further, the Vitamin C content of the vitamin c solution of the step (1) is 500mg/L-600mg/L.
Further, sodium hydroxide solution, 2mg/ that the lye of the step (1) is 1mg/L-3mg/L by mass concentration
The sodium bicarbonate solution of L-4mg/L and the gumbo polysaccharide solution of 1mg/L-3mg/L are made according to mass ratio for 1-3:2-4:1-2 mixing
?.
Further, the biological enzyme solutions of the step (1) are according to mass ratio by protease, tannase and cellulase
1:2-4:3-5 is mixed to prepare；The enzyme activity of the protease is 800u/g-1000u/g, and the enzyme activity of tannase is 800u/g-
1000u/g, the enzyme activity of cellulase are 1200u/g-1500u/g.
Further, step (3) organic acid soln is by oxalic acid solution, citric acid solution and chitosan solution according to matter
Amount is than being that 1:1-3:1-2 is formed；The oxalic acid mass concentration of the oxalic acid solution is 10-15mg/L；The citric acid of citric acid solution
Mass concentration is 5-10mg/L；The chitosan mass concentration of chitosan solution is 7-14mg/L.
Further, step (3) organic solvent is 3-4:1-2:1 according to mass ratio by ethyl alcohol, ethyl ester and n-butanol
Composition；The percentage by volume of the ethyl alcohol is 95%；The percentage by volume of the ethyl ester is 30%；The volume hundred of the n-butanol
Score is 15%.
Further, the extraction power of step (3) ultrasonic extraction is 500w-1000w.
The invention has the following beneficial effects:
It includes cowpea seed pretreatment that the cowpea anthocyanidin of the application, which extracts, blue light processing, anthocyanidin extracts and anthocyanidin
Only four steps are proposed, in preprocessing process, vitamin C can extract anthocyanidin well；Cowpea seed is mixed with vitamin C
Afterwards, it freezes, vitamin c solution volume can expand, to puncture cowpea seed cell, can promote releasing for cylinder beans seed Dissolve things inside
It puts；Lye is additionally added in preprocessing process, lye is mixed by sodium hydroxide solution, sodium bicarbonate solution and gumbo polysaccharide solution and made
, these ingredients can be enriched with anthocyanidin well, promote the synthesis of anthocyanidin intermediate, and gumbo polysaccharide can also can form coating and will spend
Green element absorption is precipitated；Biological enzyme is added after alkali process, the decomposition of macromolecular substances can be promoted, is carried out brokenly for the cell of cowpea
Wall, accelerates the precipitation of Dissolve things inside, to improve anthocyanidin yield；It is found in applicant's research process, if at this time to extraction
Liquid carry out blue light processing will further be enriched with anthocyanidin, concrete reason is also making further research at present, tentatively conclude be because
The synthesis of certain enzymatic reactions promotion anthocyanidin intermediates is promoted for blue light；After blue light illumination, by organic solvent extract and
Macroporous resin adsorption can effectively promote the precipitation and purifying of anthocyanidin, can be had using the present processes production cowpea anthocyanidin
Effect improves the content and yield of anthocyanidin in extract, while vitamin content in extract can also be effectively reduced.
[specific embodiment]
All features disclosed in this specification or disclosed all methods or in the process the step of, in addition to mutually exclusive
Feature and/or step other than, can combine in any way.
Any feature disclosed in this specification (including any accessory claim, abstract), unless specifically stated, each
Feature is an example in a series of equivalent or similar characteristics.
Embodiment 1:
A kind of cowpea anthocyanidin preparation method is present embodiments provided, this method comprises the following steps:
(1) cowpea seed pre-processes: taking the mature cowpea seed that moisture content is 2%, (vitamin C is molten with vitamin c solution
The Vitamin C content of liquid is 500mg/L) it according to mass ratio is that 1:7 is mixed, it is then placed in the refrigerator-freezer that temperature is -10 DEG C
It is anxious to freeze 60min；Thaw at RT after anxious jelly all melts to ice cube, and then according to the lye of mixeding liquid volume 5%, (lye is by matter
Measuring concentration is the gumbo polysaccharide solution of the sodium hydroxide solution of 1mg/L, the sodium bicarbonate solution of 2mg/L and 1mg/L according to quality
Than being mixed to prepare for 1:2:1), it is placed in 60 DEG C of water-bath and heats for 24 hours after standing 20min, filter to take filtrate and obtain cowpea dissolution
Object；Biological enzyme solutions (the protease that biological enzyme solutions are 800u/g by enzyme activity of its volume 5% is added into cowpea leachable
The cellulase that the tannic acid enzyme solutions and enzyme activity that solution, enzyme activity are 800u/g are 1200u/g is 1:2:3 mixed according to mass ratio
Close and be made), isothermal reaction 3h under the conditions of temperature is 36 DEG C obtains cowpea seed pretreatment fluid；
(2) blue light is handled: the cowpea seed pretreatment fluid of step (1) being put into blue light lamp box and carries out blue light illumination, is shone
A length of 50h when penetrating；The blue light intensity of illumination of irradiation is 1000Lx；
(3) anthocyanidin extract: by after step (2) blue light illumination cowpea seed pretreatment fluid and organic acid soln according to body
Than filtering after be 2:3 hybrid reaction 3h, (oxalic acid solution that organic acid soln is 10mg/L by mass concentration, mass concentration are product
The chitosan solution that the citric acid solution and mass concentration of 5mg/L is 7mg/L is that 1:1:1 is mixed to prepare according to mass ratio), take filter
Liquid is that 1:4 is mixed (ethyl alcohol, volume basis of the organic solvent by percentage by volume for 95% according to mass ratio with organic solvent
The n-butanol that the ethyl ester and percentage by volume that number is 30% are 15% is that 3:1:1 is mixed to prepare according to mass ratio), it is then placed in super
Ultrasonic extraction is carried out in sound extractor under conditions of 500w；It is thick that rotation is concentrated by evaporation after extraction 15h, drying obtains anthocyanidin
Powder；
(4) cyanine is plain mentions: the anthocyanidin coarse powder of step (3) and distilled water are mixed to get flower according to mass ratio for 1:10
Green element solution, is then adsorbed with macroporous resin column；When absorption, column flow rate is 0.5 times of column volume/small on anthocyanidin solution
When, upper column temperature is 30 DEG C, is eluted after the completion of absorption with the ethanol solution that percentage by volume is 75%, then carries out vacuum
Concentration, freeze-drying obtain the cowpea anthocyanidin；The upper column flow rate of ethanol solution is 1 times of column volume/hour when the elution.
Embodiment 2:
A kind of cowpea anthocyanidin preparation method is present embodiments provided, this method comprises the following steps:
(1) cowpea seed pre-processes: taking the mature cowpea seed that moisture content is 5%, (vitamin C is molten with vitamin c solution
The Vitamin C content of liquid is 600mg/L) it according to mass ratio is that 1:7 is mixed, it is then placed in anxious in the refrigerator-freezer that temperature is -5 DEG C
Freeze 80min；Thaw at RT after anxious jelly all melts to ice cube, and then according to the lye of mixeding liquid volume 8%, (lye is by quality
Concentration is the gumbo polysaccharide solution of the sodium hydroxide solution of 3mg/L, the sodium bicarbonate solution of 4mg/L and 3mg/L according to mass ratio
It is mixed to prepare for 3:4:2), it is placed in 70 DEG C of water-bath after standing 30min and heats 26h, filter to take filtrate and obtain cowpea leachable；
Be added into cowpea leachable its volume 8% biological enzyme solutions (biological enzyme solutions by enzyme activity be 1000u/g protease it is molten
The cellulase that the tannic acid enzyme solutions and enzyme activity that liquid, enzyme activity are 1000u/g are 1500u/g is 1:4:5 mixed according to mass ratio
Close and be made), isothermal reaction 4h under the conditions of temperature is 38 DEG C obtains cowpea seed pretreatment fluid；
(2) blue light is handled: the cowpea seed pretreatment fluid of step (1) being put into blue light lamp box and carries out blue light illumination, is shone
A length of 52h when penetrating；The blue light intensity of illumination of irradiation is 1500Lx；
(3) anthocyanidin extract: by after step (2) blue light illumination cowpea seed pretreatment fluid and organic acid soln according to body
Than filtering after be 4:5 hybrid reaction 5h, (oxalic acid solution that organic acid soln is 15mg/L by mass concentration, mass concentration are product
The chitosan solution that the citric acid solution and mass concentration of 10mg/L is 14mg/L is that 1:3:2 is mixed to prepare according to mass ratio), it takes
Filtrate and organic solvent are that 1:6 is mixed (ethyl alcohol that organic solvent is 95% by percentage by volume, volume hundred according to mass ratio
The n-butanol that the ethyl ester and percentage by volume that score is 30% are 15% is that 4:2:1 is mixed to prepare according to mass ratio), it is then placed in
Ultrasonic extraction is carried out in supersonic extractors under conditions of 1000w；Rotation is concentrated by evaporation after extraction 17h, drying obtains anthocyanidin
Coarse powder；
(4) cyanine is plain mentions: the anthocyanidin coarse powder of step (3) and distilled water are mixed to get flower according to mass ratio for 1:15
Green element solution, is then adsorbed with macroporous resin column；When absorption, column flow rate is 1 times of column volume/hour on anthocyanidin solution,
Upper column temperature is 40 DEG C, is eluted after the completion of absorption with the ethanol solution that percentage by volume is 75%, and it is dense then to carry out vacuum
Contracting, freeze-drying obtain the cowpea anthocyanidin；The upper column flow rate of ethanol solution is 1.5 times of column volume/hours when the elution.
Embodiment 3:
A kind of cowpea anthocyanidin preparation method is present embodiments provided, this method comprises the following steps:
(1) cowpea seed pre-processes: taking the mature cowpea seed that moisture content is 3%, (vitamin C is molten with vitamin c solution
The Vitamin C content of liquid is 550mg/L) it according to mass ratio is that 1:7 is mixed, it is then placed in anxious in the refrigerator-freezer that temperature is -7 DEG C
Freeze 70min；Thaw at RT after anxious jelly all melts to ice cube, and then according to the lye of mixeding liquid volume 7%, (lye is by quality
Concentration is the gumbo polysaccharide solution of the sodium hydroxide solution of 2mg/L, the sodium bicarbonate solution of 3mg/L and 2mg/L according to mass ratio
It is mixed to prepare for 2:3:2), it is placed in 65 DEG C of water-bath after standing 25min and heats 25h, filter to take filtrate and obtain cowpea leachable；
Be added into cowpea leachable its volume 6% biological enzyme solutions (biological enzyme solutions by enzyme activity be 900u/g protease it is molten
The cellulase that the tannic acid enzyme solutions and enzyme activity that liquid, enzyme activity are 900u/g are 1300u/g is 1:3:4 mixing according to mass ratio
It is made), isothermal reaction 3.5h under the conditions of temperature is 37 DEG C obtains cowpea seed pretreatment fluid；
(2) blue light is handled: the cowpea seed pretreatment fluid of step (1) being put into blue light lamp box and carries out blue light illumination, is shone
A length of 51h when penetrating；The blue light intensity of illumination of irradiation is 1200Lx；
(3) anthocyanidin extract: by after step (2) blue light illumination cowpea seed pretreatment fluid and organic acid soln according to body
Than filtering after be 3:4 hybrid reaction 4h, (oxalic acid solution that organic acid soln is 12mg/L by mass concentration, mass concentration are product
The chitosan solution that the citric acid solution and mass concentration of 8mg/L is 10mg/L is that 1:2:1 is mixed to prepare according to mass ratio), it takes
Filtrate and organic solvent are that 1:5 is mixed (ethyl alcohol that organic solvent is 95% by percentage by volume, volume hundred according to mass ratio
The n-butanol that the ethyl ester and percentage by volume that score is 30% are 15% is that 4:1:1 is mixed to prepare according to mass ratio), it is then placed in
Ultrasonic extraction is carried out in supersonic extractors under conditions of 800w；It is thick that rotation is concentrated by evaporation after extraction 16h, drying obtains anthocyanidin
Powder；
(4) cyanine is plain mentions: the anthocyanidin coarse powder of step (3) and distilled water are mixed to get flower according to mass ratio for 1:12
Green element solution, is then adsorbed with macroporous resin column；When absorption, column flow rate is 0.8 times of column volume/small on anthocyanidin solution
When, upper column temperature is 35 DEG C, is eluted after the completion of absorption with the ethanol solution that percentage by volume is 75%, then carries out vacuum
Concentration, freeze-drying obtain the cowpea anthocyanidin；The upper column flow rate of ethanol solution is 1.3 times of column volumes/small when the elution
When.
Control group 1:
In this control group vitamin c solution ascorbic content lower than 500mg/L be 400mg/L, other preparation methods with
Embodiment 1 is identical.
Control group 2:
In this control group vitamin c solution ascorbic content be higher than 600mg/L be 700mg/L, other preparation methods with
Embodiment 1 is identical.
Control group 3:
This control group pre-processes cowpea without using lye, and other preparation methods are identical with embodiment 1.
Control group 4:
This control group pre-processes cowpea without using biological enzyme, and other preparation methods are identical with embodiment 1.
Control group 5:
This control group pre-processes cowpea without using organic acid, and other preparation methods are identical with embodiment 1.
Control group 6:
This control group is handled without using blue light, and other preparation methods are identical with embodiment 1.
Control group 7:
Ethyl alcohol is used only in this control group organic solvent, that is, in the extraction of step (3) anthocyanidin: by step (2) blue light illumination
The ethanol solution that cowpea seed pretreatment clear liquid and percentage by volume afterwards is 95% is that 1:3 is mixed according to mass ratio,
Its preparation method is identical with embodiment 1.
Yield and purity test:
Anthocyanidin, tannin content in embodiment 1-3 and control group 1-5 extract are counted and detected, and calculates anthocyanidin and obtains
Rate, yield are calculated by following formula and are obtained, and concrete condition is shown in Table 1:
Table 1
As seen from the above table, the anthocyanidin content of embodiment 1-3, yield are all apparently higher than according to group 3-7, illustrate the alkali of the application
Liquid processing, cellulase treatment, organic acid processing, blue light illumination processing and organic solvent formula can improve the application well
Cowpea anthocyanidin content, and it is indispensable；The tannin content of embodiment 1-3 is significantly lower than control group 3-4 and control group 7, says
Base extraction, cellulase treatment and the organic solvent of bright the application matches the tannin content that can reduce the application well；And it is right
Control group 2 is below according to the anthocyanidin content of group 1, yield, tannin content；Illustrate, in the application vitamin C treatment process, mentions
The yield of anthocyanidin can be improved in homovitamin C component content, but excessively high vitamin C will cause tannin in cyanidin extract
Too high levels.
Influence test of the lye component to tannin content:
Cowpea seed is pre-processed according to following processing group, test pretreatment front and back cowpea seed tannin content, place
Reason group is as follows:
Processing 1: cowpea seed is pre-processed according to the method for the embodiment of the present application 1；
Processing 2: sodium hydroxide solution is free of in lye, other processing modes are identical with embodiment 1, i.e., lye is by matter
The gumbo polysaccharide solution of sodium bicarbonate solution and 1mg/L that amount concentration is 2mg/L is that 2:1 is mixed to prepare according to mass ratio.
Processing 3: sodium bicarbonate solution is free of in lye, other processing modes are identical with embodiment 1, i.e., lye is by matter
The gumbo polysaccharide solution of sodium hydroxide solution and 1mg/L that amount concentration is 1mg/L is that 1:1 is mixed to prepare according to mass ratio.
Processing 4: gumbo polysaccharide solution is free of in lye, other processing modes are identical with embodiment 1, i.e., lye is by matter
The sodium bicarbonate solution of sodium hydroxide solution and 2mg/L that amount concentration is 1mg/L is that 1:2 is mixed to prepare according to mass ratio.
Test result is shown in Table 2:
Table 2
| Tannin content (mg/g) | Processing 1 | Processing 2 | Processing 3 | Processing 4 |
| Before processing | 16.33 | 16.03 | 16.01 | 15.03 |
| After processing | 4.02 | 9.33 | 10.06 | 11.98 |
As seen from the above table, the tannin processing ability for handling 1 is greater than processing 2-4, illustrates sodium hydroxide solution, the carbon in lye
Sour hydrogen sodium solution, gumbo polysaccharide solution are mutually used in compounding, and can effectively improve the processing capacity of tannin, and its ingredient lacks one not
It can.
Influence test of the biological enzyme solution component to tannin content:
Cowpea seed is pre-processed according to following processing group, test pretreatment front and back cowpea seed tannin content, place
Reason group is as follows:
Processing A: cowpea is pre-processed according to the method for the embodiment of the present application 1；
It handles B: being free of protein enzyme solution in pretreatment fluid, other processing modes are identical with embodiment 1, i.e. biological enzyme
The cellulase that the tannic acid enzyme solutions and enzyme activity that solution is 800u/g by enzyme activity are 1200u/g is that 2:3 is mixed according to mass ratio
It closes and is made.
It handles C: being free of tannic acid enzyme solutions in pretreatment fluid, other processing modes are identical with embodiment 1, i.e. biological enzyme
The cellulase that the protein enzyme solution and enzyme activity that solution is 800u/g by enzyme activity are 1200u/g is that 1:3 is mixed according to mass ratio
It closes and is made.
It handles D: being free of cellulase solution in pretreatment fluid, other processing modes are identical with embodiment 1, i.e., biological
The tannic acid enzyme solutions that the protein enzyme solution and enzyme activity that enzyme solutions are 800u/g by enzyme activity are 800u/g are 1:3 according to mass ratio
It is mixed to prepare.
Test result is shown in Table 3:
Table 3
| Tannin content (mg/g) | Handle A | Handle B | Handle C | Handle D |
| Before processing | 16.33 | 16.03 | 16.01 | 15.03 |
| After processing | 4.02 | 8.13 | 9.56 | 9.38 |
As seen from the above table, the tannin processing ability for handling A is greater than processing B-C, illustrate protein enzyme solution in biological enzyme solution,
Tannic acid enzyme solutions, cellulase solution are mutually used in compounding, and can effectively improve the processing capacity of tannin, and its ingredient lacks one not
It can.
In conclusion the content of anthocyanidin in extract can be effectively improved using method production cowpea anthocyanidin of the invention
And yield, while tannin content in extract can also be effectively reduced.
The embodiments described above only express several embodiments of the present invention, and the description thereof is more specific and detailed, but simultaneously
Limitation of the scope of the invention therefore cannot be interpreted as.It should be pointed out that for those of ordinary skill in the art,
Without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to protection model of the invention
It encloses.Therefore, protection scope of the present invention should be determined by the appended claims.
Claims (7)
1. a kind of cowpea anthocyanidin preparation method, which is characterized in that described method includes following steps:
(1) cowpea seed pre-processes: taking the mature cowpea seed and vitamin c solution that moisture content is 2%-5% according to mass ratio
It is mixed for 1:7, is then placed in the refrigerator-freezer that temperature is -10 DEG C~-5 DEG C and suddenly freezes 60min-80min；Room temperature solution after anxious jelly
Freeze, all melt to ice cube, then according to the lye of mixeding liquid volume 5%-8%, stand be placed on after 20min-30min 60 DEG C-
- 26h for 24 hours is heated in 70 DEG C of water-bath, is filtered to take filtrate and is obtained cowpea leachable；Its volume 5%- is added into cowpea leachable
8% biological enzyme solutions, isothermal reaction 3h-4h under the conditions of temperature is 36 DEG C -38 DEG C, obtain cowpea seed pretreatment fluid；
(2) blue light is handled: the cowpea seed pretreatment fluid of step (1) being put into blue light lamp box and carries out blue light illumination, when irradiation
A length of 50h-52h；The blue light intensity of illumination of irradiation is 1000Lx-1500Lx；
(3) anthocyanidin extract: by after step (2) blue light illumination cowpea seed pretreatment fluid and organic acid soln according to volume ratio
To be filtered after 2-4:3-5 hybrid reaction 3h-5h, takes filtrate to be mixed with organic solvent according to mass ratio for 1:4-6, then put
Enter and carries out ultrasonic extraction in supersonic extractors；Rotation is concentrated by evaporation after extraction 15h-17h, drying obtains anthocyanidin coarse powder；
(4) cyanine is plain mentions: the anthocyanidin coarse powder of step (3) and distilled water are mixed to get flower according to mass ratio for 1:10-15
Green element solution, is then adsorbed with macroporous resin column；When absorption, column flow rate is 0.5-1 times of column volume/small on anthocyanidin solution
When, upper column temperature is 30-40 DEG C, is eluted after the completion of absorption with the ethanol solution that percentage by volume is 75%, is then carried out
Vacuum concentration, freeze-drying obtain the cowpea anthocyanidin；The upper column flow rate of ethanol solution is 1-1.5 times of cylinder when the elution
Product/hour.
2. cowpea anthocyanidin preparation method according to claim 1, which is characterized in that the vitamin C of the step (1) is molten
The Vitamin C content of liquid is 500mg/L-600mg/L.
3. cowpea anthocyanidin preparation method according to claim 1, which is characterized in that the lye of the step (1) is by matter
Measure sodium hydroxide solution, the sodium bicarbonate solution of 2mg/L-4mg/L and the autumn of 1mg/L-3mg/L that concentration is 1mg/L-3mg/L
Certain herbaceous plants with big flowers polysaccharide solution is mixed to prepare according to mass ratio for 1-3:2-4:1-2.
4. cowpea anthocyanidin preparation method according to claim 1, which is characterized in that the biological enzyme of the step (1) is molten
Liquid is mixed to prepare by protease, tannase and cellulase according to mass ratio for 1:2-4:3-5；The enzyme activity of the protease is
800u/g-1000u/g, the enzyme activity of tannase are 800u/g-1000u/g, and the enzyme activity of cellulase is 1200u/g-
1500u/g。
5. cowpea anthocyanidin preparation method according to claim 1, which is characterized in that step (3) organic acid soln
It is made of oxalic acid solution, citric acid solution and chitosan solution according to mass ratio for 1:1-3:1-2；The oxalic acid of the oxalic acid solution
Mass concentration is 10-15mg/L；The citric acid mass concentration of citric acid solution is 5-10mg/L；The chitosan matter of chitosan solution
Amount concentration is 7-14mg/L.
6. cowpea anthocyanidin preparation method according to claim 1, which is characterized in that step (3) organic solvent by
Ethyl alcohol, ethyl ester and n-butanol are 3-4:1-2:1 composition according to mass ratio；The percentage by volume of the ethyl alcohol is 95%；The second
The percentage by volume of ester is 30%；The percentage by volume of the n-butanol is 15%.
7. cowpea anthocyanidin preparation method according to claim 1, which is characterized in that step (3) ultrasonic extraction
Extraction power is 500w-1000w.
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| CN201811154867.7A Withdrawn CN109265426A (en) | 2018-09-30 | 2018-09-30 | A kind of cowpea anthocyanidin preparation method |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110169467A (en) * | 2019-05-15 | 2019-08-27 | 安徽省万清堂生物科技有限公司 | Functional health tea and preparation method thereof |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110169467A (en) * | 2019-05-15 | 2019-08-27 | 安徽省万清堂生物科技有限公司 | Functional health tea and preparation method thereof |
| CN110169467B (en) * | 2019-05-15 | 2021-07-02 | 安徽省万清堂生物科技有限公司 | Functional health tea and preparation method thereof |
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