CN108968066A - A kind of ginseng of strengthen immunity, Rhizoma Atractylodis Macrocephalae, Poria cocos, herbal cuisine element product of fructus lycii and preparation method thereof - Google Patents
A kind of ginseng of strengthen immunity, Rhizoma Atractylodis Macrocephalae, Poria cocos, herbal cuisine element product of fructus lycii and preparation method thereof Download PDFInfo
- Publication number
- CN108968066A CN108968066A CN201810757017.XA CN201810757017A CN108968066A CN 108968066 A CN108968066 A CN 108968066A CN 201810757017 A CN201810757017 A CN 201810757017A CN 108968066 A CN108968066 A CN 108968066A
- Authority
- CN
- China
- Prior art keywords
- herbal cuisine
- element product
- mouse
- ginseng
- fructus lycii
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 241000208340 Araliaceae Species 0.000 title claims abstract description 27
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 title claims abstract description 27
- 235000003140 Panax quinquefolius Nutrition 0.000 title claims abstract description 27
- 244000197580 Poria cocos Species 0.000 title claims abstract description 27
- 235000008599 Poria cocos Nutrition 0.000 title claims abstract description 27
- 235000008434 ginseng Nutrition 0.000 title claims abstract description 27
- 230000036039 immunity Effects 0.000 title claims abstract description 24
- 238000002360 preparation method Methods 0.000 title claims abstract description 19
- 239000000463 material Substances 0.000 claims abstract description 20
- 238000000605 extraction Methods 0.000 claims abstract description 14
- 235000011194 food seasoning agent Nutrition 0.000 claims abstract description 13
- 239000002994 raw material Substances 0.000 claims abstract description 4
- 239000004615 ingredient Substances 0.000 claims abstract description 3
- 239000000047 product Substances 0.000 claims description 57
- 239000000284 extract Substances 0.000 claims description 48
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 24
- 239000003814 drug Substances 0.000 claims description 21
- 238000000034 method Methods 0.000 claims description 17
- 239000000843 powder Substances 0.000 claims description 11
- 238000010992 reflux Methods 0.000 claims description 11
- 239000000706 filtrate Substances 0.000 claims description 10
- 235000019640 taste Nutrition 0.000 claims description 9
- 229940079593 drug Drugs 0.000 claims description 8
- 230000006837 decompression Effects 0.000 claims description 7
- 238000001035 drying Methods 0.000 claims description 5
- 238000010438 heat treatment Methods 0.000 claims description 5
- 239000007788 liquid Substances 0.000 claims description 5
- 239000000203 mixture Substances 0.000 claims description 5
- 239000002304 perfume Substances 0.000 claims description 5
- 238000011160 research Methods 0.000 claims description 5
- 230000001954 sterilising effect Effects 0.000 claims description 5
- 238000004806 packaging method and process Methods 0.000 claims description 4
- 239000000796 flavoring agent Substances 0.000 claims description 3
- 235000019634 flavors Nutrition 0.000 claims description 3
- 235000014347 soups Nutrition 0.000 claims description 3
- 238000004659 sterilization and disinfection Methods 0.000 claims description 3
- 235000013311 vegetables Nutrition 0.000 claims description 3
- 240000002234 Allium sativum Species 0.000 claims description 2
- 235000007688 Lycopersicon esculentum Nutrition 0.000 claims description 2
- 239000006002 Pepper Substances 0.000 claims description 2
- 240000003768 Solanum lycopersicum Species 0.000 claims description 2
- 238000001704 evaporation Methods 0.000 claims description 2
- 235000013373 food additive Nutrition 0.000 claims description 2
- 239000002778 food additive Substances 0.000 claims description 2
- 238000009472 formulation Methods 0.000 claims description 2
- 235000004611 garlic Nutrition 0.000 claims description 2
- 239000006210 lotion Substances 0.000 claims description 2
- 238000000643 oven drying Methods 0.000 claims description 2
- 230000002285 radioactive effect Effects 0.000 claims description 2
- 238000007493 shaping process Methods 0.000 claims description 2
- 239000007787 solid Substances 0.000 claims description 2
- 235000013599 spices Nutrition 0.000 claims description 2
- 238000001291 vacuum drying Methods 0.000 claims description 2
- 238000007654 immersion Methods 0.000 claims 2
- 239000012141 concentrate Substances 0.000 claims 1
- 235000013305 food Nutrition 0.000 abstract description 20
- 230000036541 health Effects 0.000 abstract description 15
- 230000035622 drinking Effects 0.000 abstract description 3
- 235000008216 herbs Nutrition 0.000 abstract description 3
- 230000010354 integration Effects 0.000 abstract description 3
- 230000009758 senescence Effects 0.000 abstract description 3
- 230000001256 tonic effect Effects 0.000 abstract description 3
- 238000000465 moulding Methods 0.000 abstract description 2
- 241000699666 Mus <mouse, genus> Species 0.000 description 105
- 210000002966 serum Anatomy 0.000 description 42
- 210000000952 spleen Anatomy 0.000 description 26
- 238000012360 testing method Methods 0.000 description 26
- 239000003795 chemical substances by application Substances 0.000 description 20
- 238000011049 filling Methods 0.000 description 19
- 108010006464 Hemolysin Proteins Proteins 0.000 description 18
- 239000003228 hemolysin Substances 0.000 description 18
- 210000004907 gland Anatomy 0.000 description 13
- 210000001541 thymus gland Anatomy 0.000 description 13
- 241001494479 Pecora Species 0.000 description 12
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 12
- 238000004458 analytical method Methods 0.000 description 12
- 210000003743 erythrocyte Anatomy 0.000 description 12
- 239000011780 sodium chloride Substances 0.000 description 12
- 238000007619 statistical method Methods 0.000 description 12
- 239000006228 supernatant Substances 0.000 description 12
- 238000002474 experimental method Methods 0.000 description 11
- 238000004519 manufacturing process Methods 0.000 description 11
- 239000002504 physiological saline solution Substances 0.000 description 11
- 239000000523 sample Substances 0.000 description 10
- 210000000056 organ Anatomy 0.000 description 8
- 231100000215 acute (single dose) toxicity testing Toxicity 0.000 description 7
- 239000012153 distilled water Substances 0.000 description 7
- 238000005259 measurement Methods 0.000 description 7
- 238000002791 soaking Methods 0.000 description 7
- 241000700199 Cavia porcellus Species 0.000 description 6
- CMSMOCZEIVJLDB-UHFFFAOYSA-N Cyclophosphamide Chemical compound ClCCN(CCCl)P1(=O)NCCCO1 CMSMOCZEIVJLDB-UHFFFAOYSA-N 0.000 description 6
- 241000628997 Flos Species 0.000 description 6
- 238000002835 absorbance Methods 0.000 description 6
- 210000004369 blood Anatomy 0.000 description 6
- 239000008280 blood Substances 0.000 description 6
- 210000005252 bulbus oculi Anatomy 0.000 description 6
- 239000003153 chemical reaction reagent Substances 0.000 description 6
- 210000002808 connective tissue Anatomy 0.000 description 6
- 239000013068 control sample Substances 0.000 description 6
- 229960004397 cyclophosphamide Drugs 0.000 description 6
- 230000007423 decrease Effects 0.000 description 6
- 238000002347 injection Methods 0.000 description 6
- 239000007924 injection Substances 0.000 description 6
- 239000000725 suspension Substances 0.000 description 6
- 230000002992 thymic effect Effects 0.000 description 6
- 239000003643 water by type Substances 0.000 description 6
- 230000003442 weekly effect Effects 0.000 description 6
- 238000005303 weighing Methods 0.000 description 6
- 238000001914 filtration Methods 0.000 description 5
- 235000012054 meals Nutrition 0.000 description 5
- 235000021195 test diet Nutrition 0.000 description 5
- -1 bulk) Substances 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 241001465754 Metazoa Species 0.000 description 3
- 239000006286 aqueous extract Substances 0.000 description 3
- 230000005856 abnormality Effects 0.000 description 2
- 230000033228 biological regulation Effects 0.000 description 2
- 230000000052 comparative effect Effects 0.000 description 2
- 238000010411 cooking Methods 0.000 description 2
- 235000005911 diet Nutrition 0.000 description 2
- 230000037213 diet Effects 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 239000003651 drinking water Substances 0.000 description 2
- 235000020188 drinking water Nutrition 0.000 description 2
- 239000012530 fluid Substances 0.000 description 2
- 230000001900 immune effect Effects 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- LPUQAYUQRXPFSQ-DFWYDOINSA-M monosodium L-glutamate Chemical compound [Na+].[O-]C(=O)[C@@H](N)CCC(O)=O LPUQAYUQRXPFSQ-DFWYDOINSA-M 0.000 description 2
- 235000013923 monosodium glutamate Nutrition 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 235000002722 Dioscorea batatas Nutrition 0.000 description 1
- 235000006536 Dioscorea esculenta Nutrition 0.000 description 1
- 240000001811 Dioscorea oppositifolia Species 0.000 description 1
- 235000003416 Dioscorea oppositifolia Nutrition 0.000 description 1
- 241000699670 Mus sp. Species 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 231100000403 acute toxicity Toxicity 0.000 description 1
- 230000007059 acute toxicity Effects 0.000 description 1
- 238000011047 acute toxicity test Methods 0.000 description 1
- 210000003484 anatomy Anatomy 0.000 description 1
- 238000010171 animal model Methods 0.000 description 1
- 230000036528 appetite Effects 0.000 description 1
- 235000019789 appetite Nutrition 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 230000003796 beauty Effects 0.000 description 1
- 230000037396 body weight Effects 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 235000013409 condiments Nutrition 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000000857 drug effect Effects 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 210000001508 eye Anatomy 0.000 description 1
- 230000037406 food intake Effects 0.000 description 1
- 235000012631 food intake Nutrition 0.000 description 1
- 235000013376 functional food Nutrition 0.000 description 1
- 230000002496 gastric effect Effects 0.000 description 1
- 238000003304 gavage Methods 0.000 description 1
- 210000004392 genitalia Anatomy 0.000 description 1
- 230000036449 good health Effects 0.000 description 1
- 230000008629 immune suppression Effects 0.000 description 1
- 238000001802 infusion Methods 0.000 description 1
- 210000000936 intestine Anatomy 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 229940126601 medicinal product Drugs 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 239000004223 monosodium glutamate Substances 0.000 description 1
- 210000000214 mouth Anatomy 0.000 description 1
- 210000001331 nose Anatomy 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 210000000496 pancreas Anatomy 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 239000002574 poison Substances 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 239000012925 reference material Substances 0.000 description 1
- 230000011514 reflex Effects 0.000 description 1
- 230000029058 respiratory gaseous exchange Effects 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 235000014102 seafood Nutrition 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 238000004088 simulation Methods 0.000 description 1
- 235000000891 standard diet Nutrition 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 238000002560 therapeutic procedure Methods 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
- 210000001835 viscera Anatomy 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Botany (AREA)
- Mycology (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention discloses a kind of ginsengs of strengthen immunity, Rhizoma Atractylodis Macrocephalae, Poria cocos, the herbal cuisine element product of fructus lycii and preparation method thereof.The product is by including that the component of following raw materials is prepared: ginseng, Rhizoma Atractylodis Macrocephalae, Poria cocos, fructus lycii.Wherein, by weight percentage, ginseng 10%~18%, Rhizoma Atractylodis Macrocephalae 17%~32%, Poria cocos 30%~50%, fructus lycii 17%~32%, the sum of each ingredient are 100%.The present invention, by compatibility, extraction, seasoning, molding, forms herbal cuisine element products that are a series of using tonic health as major function, and having flavouring handling characteristics based on integration of drinking and medicinal herbs medicinal material.This herbal cuisine health form of herbal cuisine element such as can not only bring delicious food for us, and have the function of strengthen immunity, delay senescence, keep fit and healthy, raising health care at a variety of health cares.
Description
Technical field
The present invention relates to food, functional food, field of health care food, and in particular to a kind of ginseng of strengthen immunity,
Rhizoma Atractylodis Macrocephalae, Poria cocos, herbal cuisine element product of fructus lycii and preparation method thereof.
Background technique
Medicative diet for food therapy is the important component of traditional Chinese medicine, is in traditional Chinese medicine, cookery and nutrition theoretical direction
Under, Chinese medicine and certain foods with medical value is compatible, using the unique diet culinary art in China and modern science
The ticbit with certain color, smell, taste, shape that method is made, while also there is extra-nutrition, nourishing and health preserving, auxiliary
It cures the disease, health body-building, the effect promoted longevity etc..What herbal cuisine used is mostly edible and medicinal product, by drug, food and
Condiment three parts composition, food is main body, supplemented by Chinese medicine is answered, not only nutritive value with higher but also has maintained the curative effect of drug,
And the characteristics such as color for having food, even if joined part medicinal material, due to having paid attention to the selection of medicinal flavor, and by with
The allotment of food and the fine cooking, still can be made into appetizing herbal cuisine.It " is combined medicine with food ", both using drug as food,
Again food is assigned to it is medicinal, medicine borrow food power, food help medicine prestige, the two complements each other, and brings out the best in each other.Actively carry out drug food of TCM dietotherapy
Health care, the good health and a long life of only Chinese people are not made that significant contribution, and the hair for promoting world health physical-fitness medicine
Exhibition, it may have far reaching significance.
Herbal cuisine element, by compatibility, extraction, seasoning, molding, is formed a series of with tonic based on integration of drinking and medicinal herbs medicinal material
Health is major function, and the product with flavouring handling characteristics such as monosodium glutamate (monosodium glutamates).
Summary of the invention
An object of the present invention is to provide a kind of ginseng, Rhizoma Atractylodis Macrocephalae, Poria cocos, the herbal cuisine element product of fructus lycii and its preparation side
Method.
Herbal cuisine element product provided by the present invention, be by include following raw materials component be prepared: ginseng, Rhizoma Atractylodis Macrocephalae,
Poria cocos, fructus lycii.
Wherein, by weight percentage, ginseng 10%~18%, Rhizoma Atractylodis Macrocephalae 17%~32%, Poria cocos 30%~50%, fructus lycii
Son 17%~32%, the sum of each ingredient are 100%.
Specifically, in the herbal cuisine element product,
(1) ginseng 12%, Rhizoma Atractylodis Macrocephalae 24%, Poria cocos 40%, fructus lycii 24%；
Or, (2) ginseng 18%, Rhizoma Atractylodis Macrocephalae 32%, Poria cocos 30%, fructus lycii 20%；
Or, (3) ginseng 18%, Rhizoma Atractylodis Macrocephalae 17%, Poria cocos 33%, fructus lycii 32%；
Or, (4) ginseng 10%, Rhizoma Atractylodis Macrocephalae 32%, Poria cocos 41%, fructus lycii 17%.
Or, (5) ginseng 10%, Rhizoma Atractylodis Macrocephalae 17%, Poria cocos 45%, fructus lycii 28%；
The present invention also provides the preparation methods of above-mentioned herbal cuisine element product, comprising the following steps:
Ginseng, Rhizoma Atractylodis Macrocephalae, Poria cocos and fructus lycii are mixed by formulation weight percentage, is soaked in water, is heated to reflux and mentions
It takes, filters, merging filtrate, be concentrated, it is dry, extract dry extract is obtained, the extract dry extract is broken into powder and is obtained.
In the above method, the ginseng, Rhizoma Atractylodis Macrocephalae, Poria cocos and fructus lycii are its pharmaceutical decocting piece.
The proportion for impregnating water used and ginseng, Rhizoma Atractylodis Macrocephalae, Poria cocos and fructus lycii mixture can are as follows: 7-11mL:1g, concretely
10mL:1g.
The soaking temperature is room temperature, and soaking time can are as follows: 1-3.5h, concretely 2.5h.
The heating and refluxing extraction can carry out 1-3 times, and concretely 3 times, the time extracted every time can be 1-2 hours, tool
Body can be 1 hour.
The operation of the heating and refluxing extraction is concretely: heating and refluxing extraction 3 times, extracting 1h every time, merges 3 gained
Aqueous extracts.
The operation that the concentration filtrate obtains extract dry extract can are as follows: the filtrate decompression is concentrated into thick paste, is shifted
It is dry into evaporating dish, obtain extract dry extract.
The reduced pressure can be 60-80 DEG C in temperature, carry out under vacuum degree -0.07~-0.09MPa.It is concentrated into medicinal extract
Relative density is 1.2~1.4；The reference material of the relative density is water.
The drying can be vacuum drying or oven drying, and drying temperature can be 55-60 DEG C.
In the pulverising step of the extract dry extract, smashed powder mesh number is 60-100 mesh；Concretely 80 mesh.
Said extracted object dry extract powder can directly sterilize after packaging without seasoning；
Alternatively, needing to be added corresponding seasoning according to taste and food additives are seasoned, and shaping auxiliary materials are added
Technological forming is carried out, sterilizes after packaging, obtains the herbal cuisine element product of various tastes；
It can be according to the market orientation and demand of product, the market distributional region feature of marketing strategy and future etc., exploitation
A series of formula of various special favors out, the taste are chosen in particular from fresh perfume, spices, garlic perfume, paste flavor, sour-sweet, sour refreshing, perfume
At least one of peppery, spicy, vinegar-pepper, curried, seafood and tomato；
The sterilizing uses irradiation sterilization method.
Specifically, it uses dosage to carry out irradiation to packaged herbal cuisine element product for the radioactive element Co60 of 3kGy to go out
Bacterium.
Above-mentioned herbal cuisine element product may also be fabricated which various forms, such as solid (powder, particle, bulk), liquid, lotion etc..
Above-mentioned herbal cuisine element product in strengthen immunity and preparation strengthen immunity, delay senescence, keep fit and healthy, raise health care
Product in application also belong to protection scope of the present invention.
It is a further object of the present invention to provide the application methods of above-mentioned herbal cuisine element product.
The application method of herbal cuisine element product provided by the present invention, comprising:
Above-mentioned herbal cuisine element product is directly added into vegetable or soup for seasoning and is eaten.
Specifically, it is put into before taking the dish out of the pot in cooking, Baoshang, boiling；Or it is direct when adjusting filling, mixing cold dish, production wheaten food
It is put into, mixes thoroughly.
Coming-of-Age Day research on maximum utilized quantity is 2g/ times, 3 times/day, is amounted to 6g/ days, wherein people calculates according to 70kg weight.
The present invention is on the basis of Chinese Traditional Medicine herbal cuisine theory, based on integration of drinking and medicinal herbs medicinal material, by compatibility, mentions
It takes, season, form, form herbal cuisine element products a series of using tonic health as major function, and that there are flavouring handling characteristics,
It can be used as seasoning to be directly added into vegetable, eat in soup.This herbal cuisine health form of herbal cuisine element, can not only bring beauty for us
Taste delicacies, and a variety of health cares such as have the function of strengthen immunity, delay senescence, keep fit and healthy, raising health care.
Specific embodiment
The present invention is further elaborated combined with specific embodiments below, but the present invention is not limited to following embodiments.Institute
State method is conventional method unless otherwise instructed.The raw material can obtain unless otherwise instructed from public commercial source.
The preparation of embodiment 1, herbal cuisine element product
Each pharmaceutical decocting piece: ginseng 36g is weighed according to following weight, and Rhizoma Atractylodis Macrocephalae 72g, Poria cocos 120g, fructus lycii 72g, investment mentions
In extracting container, add 10 times of amount distilled water, soaking at room temperature 2.5h is heated at reflux extraction 3 times, extracts 1h every time.Merge 3 water to mention
The medicinal extract that (70 DEG C, -0.07~-0.09MPa) are 1.2-1.4 to relative density, 60 degree of vacuum are concentrated in liquid, filtering, filtrate decompression
It is dry, obtain extract dry extract.Herbal cuisine extract is ground into powder (80 mesh), without seasoning, obtains the production of herbal cuisine element
Product 1.
The preparation of embodiment 2, herbal cuisine element product
Each pharmaceutical decocting piece: ginseng 54g, Rhizoma Atractylodis Macrocephalae 96g, Poria cocos 90g, fructus lycii 60g, investment extraction are weighed according to following weight
In container, add 10 times of amount distilled water, soaking at room temperature 2.5h is heated at reflux extraction 3 times, extracts 1h every time.Merge 3 Aqueous extracts,
The medicinal extract that (70 DEG C, -0.07~-0.09MPa) are 1.2-1.4 to relative density is concentrated in filtering, filtrate decompression, and 60 degree of vacuum are dry
It is dry, obtain extract dry extract.Herbal cuisine extract is ground into powder (80 mesh), without seasoning, obtains herbal cuisine element product
2。
The preparation of embodiment 3, herbal cuisine element product
Each pharmaceutical decocting piece: ginseng 54g, Rhizoma Atractylodis Macrocephalae 51g, Poria cocos 99g, fructus lycii 96g, investment extraction are weighed according to following weight
In container, add 10 times of amount distilled water, soaking at room temperature 2.5h is heated at reflux extraction 3 times, extracts 1h every time.Merge 3 Aqueous extracts,
The medicinal extract that (70 DEG C, -0.07~-0.09MPa) are 1.2-1.4 to relative density is concentrated in filtering, filtrate decompression, and 60 degree of vacuum are dry
It is dry, obtain extract dry extract.Herbal cuisine extract is ground into powder (80 mesh), without seasoning, obtains herbal cuisine element product
3。
The preparation of embodiment 4, herbal cuisine element product
Each pharmaceutical decocting piece: ginseng 30g is weighed according to following weight, and Rhizoma Atractylodis Macrocephalae 96g, Poria cocos 123g, fructus lycii 51g, investment mentions
In extracting container, add 10 times of amount distilled water, soaking at room temperature 2.5h is heated at reflux extraction 3 times, extracts 1h every time.Merge 3 water to mention
The medicinal extract that (70 DEG C, -0.07~-0.09MPa) are 1.2-1.4 to relative density, 60 degree of vacuum are concentrated in liquid, filtering, filtrate decompression
It is dry, obtain extract dry extract.Herbal cuisine extract is ground into powder (80 mesh), without seasoning, obtains the production of herbal cuisine element
Product 4.
The preparation of embodiment 5, herbal cuisine element product
Each pharmaceutical decocting piece: ginseng 30g is weighed according to following weight, and Rhizoma Atractylodis Macrocephalae 51g, Poria cocos 135g, fructus lycii 84g, investment mentions
In extracting container, add 10 times of amount distilled water, soaking at room temperature 2.5h is heated at reflux extraction 3 times, extracts 1h every time.Merge 3 water to mention
The medicinal extract that (70 DEG C, -0.07~-0.09MPa) are 1.2-1.4 to relative density, 60 degree of vacuum are concentrated in liquid, filtering, filtrate decompression
It is dry, obtain extract dry extract.Herbal cuisine extract is ground into powder (80 mesh), without seasoning, obtains the production of herbal cuisine element
Product 5.
Embodiment 6, acute toxicity testing
In order to investigate herbal cuisine element acute toxicity size, after spy cooks herbal cuisine element product sample according to application method, carry out
Acute toxicity test provides foundation to evaluate its safety for clinical application research.
6.1 experimental material
6.1.1 animal
ICR mouse, SPF grades, 18 ± 2g of weight, half male and half female is provided by Si Beifu (Beijing) Bioisystech Co., Ltd,
Animal credit number: SCXK (capital) 2016-0002.
6.1.2 drug
It is given the test agent with herbal cuisine element product 1.
6.1.3 experimental situation
Life Sciences of Beijing Institute of Technology experimental animal barrier system.
6.2 experimental method
6.2.1 preliminary experiment
ICR mouse 8 are taken, is randomly divided into two groups, i.e. control group and administration group, every group 4, half male and half female.Take herbal cuisine plain
It is made into maximum concentration medical fluid 45% (mass volume ratio), gastric infusion 3 times, every minor tick 4h, each stomach-filling volume is 0.4ml/
10g, control group stomach-filling equal amount of distilled water.12h is deprived of food but not water before stomach-filling, 7d is observed continuously in conventinal breeding after administration.Have no small
Mouse death and toxic reaction, are limited in view of by tested concentration and volume, carry out the measurement of maximum dosage-feeding experiment.
6.2.2 maximum dosage-feeding measures
ICR mouse 24 are taken, is randomly divided into two groups, i.e. control group and administration group, every group 12, half male and half female, before experiment
It is deprived of food but not water 12h, interim is not for food.Every mouse of administration group presses maximum concentration medical fluid 45% (mass volume ratio) stomach-filling
Herbal cuisine element 3 times, every minor tick 4h is given, stomach-filling volume is 0.4ml/10g, and control group gives same volume distilled water.Connect after administration
Continuous observation 14d, appearance action, the reflex activity, amount of drinking water, stool and urine situation of mouse after observation administration, after administration the 1st, 3,
5, mouse appetite and weight are measured 7,14 days, respectively, data withIt indicates, is as a result carried out at statistics with SPSS17.0 software
Reason.Gross anatomy is carried out after observation, observes the variation such as volume, color, quality of each organ.
6.3 experimental result
Acute toxicity testing the results are shown in Table 1, table 2, table 3, table 4.
The variation of 1 herbal cuisine element acute toxicity testing mouse weight of table (N=6)
The variation of 2 herbal cuisine element acute toxicity testing mouse food ration of table (G/10g weight, n=6)
The variation of 3 herbal cuisine element acute toxicity testing mouse amount of drinking water of table (Ml/10g weight, n=6)
4 herbal cuisine element acute toxicity testing mouse response situation of table
Note: "-": it is normal.
After experiment, 2 groups of mouse are dissected, visually observes, finds liver, kidney, spleen, intestines, stomach, the pancreas of all mouse
Gland, genitals official rank internal organs are normal, administration group no abnormality seen compared with blank.
The experimental results showed that animal has no dead, and general status is good after administration in 14 days, two is just normal, and appearance,
The body weight increase of fur, behavior, breathing normal, nose, eye, oral cavity secretion without exception, administration group and control group is without significance difference
It is different.Also there was no significant difference for food-intake, reaction that each organ is normal, and no other abnormalities were observed.Herbal cuisine element is given by intragastric administration on mice
Medicine, 1 day three times maximum dosage-feeding be 54g/kg, be equivalent to adult clinical day research on maximum utilized quantity (according to the daily research on maximum utilized quantity of people:
2g/ times, 3 times/day, amount to 6g/ day, people according to 70kg weight calculating) 630 times.It is therefore contemplated that the safe nothing of herbal cuisine element
Poison.
Embodiment 7, herbal cuisine element product inhibit the immunological regulation of mouse to test immunity
It is given the test agent with herbal cuisine element product 1, takes SPF grades of female BAl BIcs of 50 6-8 week old/c mouse, press standard before test
Diets one week.50 mouse are randomly divided into 5 groups after a week by pre- raise, and every group 10, grouping situation is shown in Table 5.Blank control
Group mouse peritoneal injects 0.2mL physiological saline, and other each group mouse peritoneals inject 0.2mL cyclophosphamide (mg/kg), 1 time a day,
For three days on end, manufacture mouse immune inhibits model.
Since the 4th day, into the formal tested phase.According to the present invention sample human body recommend dosage be 2g/ times, 3 times/day,
6g/ days total, people calculates according to 70kg weight, converts mouse dose, and the dosage of given the test agent low, middle and high dose groups is respectively
0.78,1.56,3.12g/kg weight, the daily stomach-filling of given the test agent is primary, gives 28 days.Blank control and model control group mouse
0.2mL sterile saline is gavaged every time, is amounted to 28 days.
5 experimental group of table
Mouse body index analysis
Mouse weight, record mouse weight variation are periodically weighed weekly.After filling four weeks, after cervical dislocation puts to death mouse,
It wins gland and board is dirty, surrounding connective tissue is stripped into clean rear weighing, data are shown in Table 6.Computation immunity organ refers to as follows
Number:
Index and spleen index=spleen weight (mg)/weight (g)
Gland index=thymic weight (mg)/weight (g)
The variation of 6 mouse weight of table and thymus index, spleen index measurement result
Note: " a ": there is significant difference, P < 0.05 compared with A blank control group.
Through statistical analysis: other each groups are compared with blank control group, and changes of weight is without significant difference；Model control group
Thymus index, spleen index significantly reduce (P < 0.05) compared with blank control group, illustrate modeling success.Various dose group and model
Control group is compared to no significant difference.
Serum hemolysin content analysis
When giving the 23rd day of tested material, 2% silk floss of every mouse peritoneal injection 0.2mL sterile saline preparation
Sheep erythrocyte suspension is immunoreacted.
After tested material is given in stopping (i.e. the 29th day), eyeball of mouse takes blood to be placed in 1.5mL centrifuge tube, and serum is precipitated
And it is diluted to suitable multiple.The guinea pig serum of the sheep red blood cell (SRBC) and 1mL10% that take dilute serum 1mL and 0.5mL10% is mixed
It closes, 30min is kept the temperature in 37 DEG C of waters bath with thermostatic control, ice bath terminates reaction, and 2000r/min is centrifuged 10min, takes supernatant 1mL, is added
3mL Dou Shi reagent, places 10min after shaking up, and absorbance is surveyed at 540nm wavelength, the results are shown in Table 7.It is replaced with physiological saline small
Mouse serum takes the zeroing in colorimetric of its supernatant as blank control.
7 serum hemolysin experimental result of table
Note: " a ": there is significant difference compared with A blank control group, " b " there were significant differences compared with B model control group, P
<0.05。
Through statistical analysis: the significant work decline of B group model control group antibody product compared with A blank control group；Low dose of C group
(0.78g/kgD) group and model control group are measured without significant difference；Middle dosage (1.56g/kgD) group and high dose (3.12g/
KgD) group dramatically increases compared with model control group.
It is drawn a conclusion by zoopery, middle dose group and the experiment of high dose group serum hemolysin are positive, medicine of the invention
Meals element has the function of strengthen immunity.
It is given the test agent with herbal cuisine element product 2, takes SPF grades of female BAl BIcs of 50 6-8 week old/c mouse, press standard before test
Diets one week.50 mouse are randomly divided into 5 groups after a week by pre- raise, and every group 10, grouping situation is shown in Table 8.Blank control
Group mouse peritoneal injects 0.2mL physiological saline, and other each group mouse peritoneals inject 0.2mL cyclophosphamide (mg/kg), 1 time a day,
For three days on end, manufacture mouse immune inhibits model.
Since the 4th day, into the formal tested phase.According to the present invention sample human body recommend dosage be 2g/ times, 3 times/day,
6g/ days total, people calculates according to 70kg weight, converts mouse dose, and the dosage of given the test agent low, middle and high dose groups is respectively
0.78,1.56,3.12g/kg weight, the daily stomach-filling of given the test agent is primary, gives 28 days.Blank control and model control group mouse
0.2mL sterile saline is gavaged every time, is amounted to 28 days.
8 experimental group of table
Mouse body index analysis
Mouse weight, record mouse weight variation are periodically weighed weekly.After filling four weeks, after cervical dislocation puts to death mouse,
It wins gland and board is dirty, surrounding connective tissue is stripped into clean rear weighing, data are shown in Table 9.Computation immunity organ refers to as follows
Number:
Index and spleen index=spleen weight (mg)/weight (g)
Gland index=thymic weight (mg)/weight (g)
The variation of 9 mouse weight of table and thymus index, spleen index measurement result
Note: " a ": there is significant difference, P < 0.05 compared with A blank control group.
Through statistical analysis: blank control group is compared with other groups, and changes of weight is without significant difference；Model control group
Thymus index, spleen index significantly reduce (P < 0.05) compared with blank control group, illustrate modeling success.Various dose group and model
Control group is compared to no significant difference.
Serum hemolysin content analysis
When giving the 23rd day of tested material, 2% silk floss of every mouse peritoneal injection 0.2mL sterile saline preparation
Sheep erythrocyte suspension is immunoreacted.
After tested material is given in stopping (i.e. the 29th day), eyeball of mouse takes blood to be placed in 1.5mL centrifuge tube, and serum is precipitated
And it is diluted to suitable multiple.The guinea pig serum of the sheep red blood cell (SRBC) and 1mL10% that take dilute serum 1mL and 0.5mL10% is mixed
It closes, 30min is kept the temperature in 37 DEG C of waters bath with thermostatic control, ice bath terminates reaction, and 2000r/min is centrifuged 10min, takes supernatant 1mL, is added
3mL Dou Shi reagent, places 10min after shaking up, and absorbance is surveyed at 540nm wavelength, the results are shown in Table 10.It is replaced with physiological saline small
Mouse serum takes the zeroing in colorimetric of its supernatant as blank control.
10 serum hemolysin experimental result of table
Note: " a ": there is significant difference compared with A blank control group, " b " there were significant differences compared with B model control group, P
<0.05。
Through statistical analysis: the significant work decline of B group model control group antibody product compared with A blank control group；Low dose of C group
(0.78g/kgD) group and model control group are measured without significant difference；Middle dosage (1.56g/kgD) group and high dose (3.12g/
KgD) group dramatically increases compared with model control group.
It is drawn a conclusion by zoopery, middle dose group and the experiment of high dose group serum hemolysin are positive, medicine of the invention
Meals element has the function of strengthen immunity.
It is given the test agent with herbal cuisine element product 3, takes SPF grades of female BAl BIcs of 50 6-8 week old/c mouse, press standard before test
Diets one week.50 mouse are randomly divided into 5 groups after a week by pre- raise, and every group 10, grouping situation is shown in Table 11.Blank control
Group mouse peritoneal injects 0.2mL physiological saline, and other each group mouse peritoneals inject 0.2mL cyclophosphamide (mg/kg), 1 time a day,
For three days on end, manufacture mouse immune inhibits model.
Since the 4th day, into the formal tested phase.According to the present invention sample human body recommend dosage be 2g/ times, 3 times/day,
6g/ days total, people calculates according to 70kg weight, converts mouse dose, and the dosage of given the test agent low, middle and high dose groups is respectively
0.78,1.56,3.12g/kg weight, the daily stomach-filling of given the test agent is primary, gives 28 days.Blank control and model control group mouse
0.2mL sterile saline is gavaged every time, is amounted to 28 days.
11 experimental group of table
Mouse body index analysis
Mouse weight, record mouse weight variation are periodically weighed weekly.After filling four weeks, after cervical dislocation puts to death mouse,
It wins gland and board is dirty, surrounding connective tissue is stripped into clean rear weighing, data are shown in Table 12.Computation immunity organ refers to as follows
Number:
Index and spleen index=spleen weight (mg)/weight (g)
Gland index=thymic weight (mg)/weight (g)
The variation of 12 mouse weight of table and thymus index, spleen index measurement result
Note: " a ": there is significant difference, P < 0.05 compared with A blank control group.
Through statistical analysis: blank control group is compared with other groups, and changes of weight is without significant difference；Model control group
Thymus index, spleen index significantly reduce (P < 0.05) compared with blank control group, illustrate modeling success.Various dose group and model
Control group is compared to no significant difference.
Serum hemolysin content analysis
When giving the 23rd day of tested material, 2% silk floss of every mouse peritoneal injection 0.2mL sterile saline preparation
Sheep erythrocyte suspension is immunoreacted.
After tested material is given in stopping (i.e. the 29th day), eyeball of mouse takes blood to be placed in 1.5mL centrifuge tube, and serum is precipitated
And it is diluted to suitable multiple.The guinea pig serum of the sheep red blood cell (SRBC) and 1mL10% that take dilute serum 1mL and 0.5mL10% is mixed
It closes, 30min is kept the temperature in 37 DEG C of waters bath with thermostatic control, ice bath terminates reaction, and 2000r/min is centrifuged 10min, takes supernatant 1mL, is added
3mL Dou Shi reagent, places 10min after shaking up, and absorbance is surveyed at 540nm wavelength, the results are shown in Table 13.It is replaced with physiological saline small
Mouse serum takes the zeroing in colorimetric of its supernatant as blank control.
13 serum hemolysin experimental result of table
Note: " a ": there is significant difference compared with A blank control group, " b " there were significant differences compared with B model control group, P
<0.05。
Through statistical analysis: the significant work decline of B group model control group antibody product compared with A blank control group；Low dose of C group
(0.78g/kgD) group and model control group are measured without significant difference；Middle dosage (1.56g/kgD) group and high dose (3.12g/
KgD) group dramatically increases compared with model control group.
It is drawn a conclusion by zoopery, middle dose group and the experiment of high dose group serum hemolysin are positive, medicine of the invention
Meals element has the function of strengthen immunity.
It is given the test agent with herbal cuisine element product 4, takes SPF grades of female BAl BIcs of 50 6-8 week old/c mouse, press standard before test
Diets one week.50 mouse are randomly divided into 5 groups after a week by pre- raise, and every group 10, grouping situation is shown in Table 14.Blank control
Group mouse peritoneal injects 0.2mL physiological saline, and other each group mouse peritoneals inject 0.2mL cyclophosphamide (mg/kg), 1 time a day,
For three days on end, manufacture mouse immune inhibits model.
Since the 4th day, into the formal tested phase.According to the present invention sample human body recommend dosage be 2g/ times, 3 times/day,
6g/ days total, people calculates according to 70kg weight, converts mouse dose, and the dosage of given the test agent low, middle and high dose groups is respectively
0.78,1.56,3.12g/kg weight, the daily stomach-filling of given the test agent is primary, gives 28 days.Blank control and model control group mouse
0.2mL sterile saline is gavaged every time, is amounted to 28 days.
14 experimental group of table
Mouse body index analysis
Mouse weight, record mouse weight variation are periodically weighed weekly.After filling four weeks, after cervical dislocation puts to death mouse,
It wins gland and board is dirty, surrounding connective tissue is stripped into clean rear weighing, data are shown in Table 15.Computation immunity organ refers to as follows
Number:
Index and spleen index=spleen weight (mg)/weight (g)
Gland index=thymic weight (mg)/weight (g)
The variation of 15 mouse weight of table and thymus index, spleen index measurement result
Note: " a ": there is significant difference, P < 0.05 compared with A blank control group.
Through statistical analysis: blank control group is compared with other groups, and changes of weight is without significant difference；Model control group
Thymus index, spleen index significantly reduce (P < 0.05) compared with blank control group, illustrate modeling success.Various dose group and model
Control group is compared to no significant difference.
Serum hemolysin content analysis
When giving the 23rd day of tested material, 2% silk floss of every mouse peritoneal injection 0.2mL sterile saline preparation
Sheep erythrocyte suspension is immunoreacted.
After tested material is given in stopping (i.e. the 29th day), eyeball of mouse takes blood to be placed in 1.5mL centrifuge tube, and serum is precipitated
And it is diluted to suitable multiple.The guinea pig serum of the sheep red blood cell (SRBC) and 1mL10% that take dilute serum 1mL and 0.5mL10% is mixed
It closes, 30min is kept the temperature in 37 DEG C of waters bath with thermostatic control, ice bath terminates reaction, and 2000r/min is centrifuged 10min, takes supernatant 1mL, is added
3mL Dou Shi reagent, places 10min after shaking up, and absorbance is surveyed at 540nm wavelength, the results are shown in Table 16.It is replaced with physiological saline small
Mouse serum takes the zeroing in colorimetric of its supernatant as blank control.
16 serum hemolysin experimental result of table
Note: " a ": there is significant difference compared with A blank control group, " b " there were significant differences compared with B model control group, P
<0.05。
Through statistical analysis: the significant work decline of B group model control group antibody product compared with A blank control group；Low dose of C group
(0.78g/kgD) group and model control group are measured without significant difference；Middle dosage (1.56g/kgD) group and high dose (3.12g/
KgD) group dramatically increases compared with model control group.
It is drawn a conclusion by zoopery, middle dose group and the experiment of high dose group serum hemolysin are positive, medicine of the invention
Meals element has the function of strengthen immunity.
It is given the test agent with herbal cuisine element product 5, takes SPF grades of female BAl BIcs of 50 6-8 week old/c mouse, press standard before test
Diets one week.50 mouse are randomly divided into 5 groups after a week by pre- raise, and every group 10, grouping situation is shown in Table 17.Blank control
Group mouse peritoneal injects 0.2mL physiological saline, and other each group mouse peritoneals inject 0.2mL cyclophosphamide (mg/kg), 1 time a day,
For three days on end, manufacture mouse immune inhibits model.
Since the 4th day, into the formal tested phase.According to the present invention sample human body recommend dosage be 2g/ times, 3 times/day,
6g/ days total, people calculates according to 70kg weight, converts mouse dose, and the dosage of given the test agent low, middle and high dose groups is respectively
0.78,1.56,3.12g/kg weight, the daily stomach-filling of given the test agent is primary, gives 28 days.Blank control and model control group mouse
0.2mL sterile saline is gavaged every time, is amounted to 28 days.
17 experimental group of table
Mouse body index analysis
Mouse weight, record mouse weight variation are periodically weighed weekly.After filling four weeks, after cervical dislocation puts to death mouse,
It wins gland and board is dirty, surrounding connective tissue is stripped into clean rear weighing, data are shown in Table 18.Computation immunity organ refers to as follows
Number:
Index and spleen index=spleen weight (mg)/weight (g)
Gland index=thymic weight (mg)/weight (g)
The variation of 18 mouse weight of table and thymus index, spleen index measurement result
Note: " a ": there is significant difference, P < 0.05 compared with A blank control group.
Through statistical analysis: blank control group is compared with other groups, and changes of weight is without significant difference；Model control group
Thymus index, spleen index significantly reduce (P < 0.05) compared with blank control group, illustrate modeling success.Various dose group and model
Control group is compared to no significant difference.
Serum hemolysin content analysis
When giving the 23rd day of tested material, 2% silk floss of every mouse peritoneal injection 0.2mL sterile saline preparation
Sheep erythrocyte suspension is immunoreacted.
After tested material is given in stopping (i.e. the 29th day), eyeball of mouse takes blood to be placed in 1.5mL centrifuge tube, and serum is precipitated
And it is diluted to suitable multiple.The guinea pig serum of the sheep red blood cell (SRBC) and 1mL10% that take dilute serum 1mL and 0.5mL10% is mixed
It closes, 30min is kept the temperature in 37 DEG C of waters bath with thermostatic control, ice bath terminates reaction, and 2000r/min is centrifuged 10min, takes supernatant 1mL, is added
3mL Dou Shi reagent, places 10min after shaking up, and absorbance is surveyed at 540nm wavelength, the results are shown in Table 19.It is replaced with physiological saline small
Mouse serum takes the zeroing in colorimetric of its supernatant as blank control.
19 serum hemolysin experimental result of table
Note: " a ": there is significant difference compared with A blank control group, " b " there were significant differences compared with B model control group, P
<0.05。
Through statistical analysis: the significant work decline of B group model control group antibody product compared with A blank control group；Low dose of C group
(0.78g/kgD) group and model control group are measured without significant difference；Middle dosage (1.56g/kgD) group and high dose (3.12g/
KgD) group dramatically increases compared with model control group.
It is drawn a conclusion by zoopery, middle dose group and the experiment of high dose group serum hemolysin are positive, medicine of the invention
Meals element has the function of strengthen immunity.
Embodiment 8, herbal cuisine element product inhibit the immunological regulation drug effect comparative test of mouse to immunity
Using the high dose of herbal cuisine element product 1 as given the test agent, the formula conduct pair by rhizoma polygonati, fructus lycii, Radix Angelicae Sinensis, Chinese yam is taken
Product in the same old way carry out the comparative efficacy test of strengthen immunity under Isodose.
SPF grades of female BAl BIcs of 40 6-8 week old/c mouse is taken, is raised one week before test by standard diet.It is pre- to raise after a week
40 mouse are randomly divided into 4 groups, every group 10, grouping situation is shown in Table 20.0.2mL physiology is injected intraperitoneally in blank control group mouse
Salt water, other each group mouse peritoneals inject 0.2mL cyclophosphamide (mg/kg), 1 time a day, for three days on end, manufacture mouse immune suppression
Simulation.
Since the 4th day, into the formal tested phase.According to the present invention sample human body recommend dosage be 2g/ times, 3 times/day,
6g/ days total, people calculates according to 70kg weight, converts mouse dose, and given the test agent dosage is set as 3.12g/kg weight, test sample
The daily stomach-filling of product is primary, gives 28 days.Control sample dosage is equally set as 3.12g/kg weight, and daily stomach-filling is primary, gives 28
It.Blank control and model control group mouse gavage 0.2mL sterile saline every time, amount to 28 days.
20 experimental group of table
Mouse body index analysis
Mouse weight, record mouse weight variation are periodically weighed weekly.After filling four weeks, after cervical dislocation puts to death mouse,
It wins gland and board is dirty, surrounding connective tissue is stripped into clean rear weighing, data are shown in Table 21.Computation immunity organ refers to as follows
Number:
Index and spleen index=spleen weight (mg)/weight (g)
Gland index=thymic weight (mg)/weight (g)
The variation of 21 mouse weight of table and thymus index, spleen index measurement result
Note: " a ": there is significant difference, P < 0.05 compared with A blank control group.
Through statistical analysis: other groups are compared with blank control group, and changes of weight is without significant difference；Model control group
Thymus index, spleen index significantly reduce (P < 0.05) compared with blank control group, illustrate modeling success.Given the test agent refers to thymus gland
Several improvement with index and spleen index, act on compared with control sample and being dominant, but without significant difference.
Serum hemolysin content analysis
When giving the 23rd day of tested material, 2% silk floss of every mouse peritoneal injection 0.2mL sterile saline preparation
Sheep erythrocyte suspension is immunoreacted.
After tested material is given in stopping (i.e. the 29th day), eyeball of mouse takes blood to be placed in 1.5mL centrifuge tube, and serum is precipitated
And it is diluted to suitable multiple.The guinea pig serum of the sheep red blood cell (SRBC) and 1mL10% that take dilute serum 1mL and 0.5mL10% is mixed
It closes, 30min is kept the temperature in 37 DEG C of waters bath with thermostatic control, ice bath terminates reaction, and 2000r/min is centrifuged 10min, takes supernatant 1mL, is added
3mL Dou Shi reagent, places 10min after shaking up, and absorbance is surveyed at 540nm wavelength, the results are shown in Table 22.It is replaced with physiological saline small
Mouse serum takes the zeroing in colorimetric of its supernatant as blank control.
22 serum hemolysin experimental result of table
Note: " a ": there is significant difference compared with A blank control group, " b " there were significant differences compared with B model control group, P
< 0.05, " c " there were significant differences compared with D control sample group, P < 0.05.
Through statistical analysis: the significant work decline of B group model control group antibody product compared with A blank control group；C group and D
Group dramatically increases compared with model control group, has statistical difference.C group given the test agent has compared with D group control sample
Significant improvement result；
It is drawn a conclusion by zoopery, test sample group is positive with the experiment of control sample group serum hemolysin, and tested
The effect of sample sets is better than control sample group, has statistical difference.Therefore there is herbal cuisine element of the invention preferably enhancing to exempt from
The function of epidemic disease power.
Claims (10)
1. a kind of herbal cuisine element product is by including that the component of following raw materials is prepared: ginseng, Rhizoma Atractylodis Macrocephalae, Poria cocos, fructus lycii；
Wherein, by weight percentage, ginseng 10%~18%, Rhizoma Atractylodis Macrocephalae 17%~32%, Poria cocos 30%~50%, fructus lycii
17%~32%, the sum of each ingredient is 100%.
2. the method for preparing herbal cuisine element product described in claim 1, comprising:
Ginseng, Rhizoma Atractylodis Macrocephalae, Poria cocos and fructus lycii are mixed by formulation weight percentage, are soaked in water, heating and refluxing extraction, mistake
Filter, merging filtrate are concentrated, dry, obtain extract dry extract, the extract dry extract is broken into powder and is obtained.
3. according to the method described in claim 2, it is characterized by: the ginseng, Rhizoma Atractylodis Macrocephalae, Poria cocos and fructus lycii are its medicinal material
Medicine materical crude slice；
Impregnate the proportion of water and ginseng, Rhizoma Atractylodis Macrocephalae, Poria cocos and fructus lycii mixture used are as follows: 7-11mL:1g；
The time of the immersion are as follows: 1-3.5h；
The temperature of the immersion is room temperature；
The heating and refluxing extraction carries out 1-3 times；Specially 3 times；The time extracted every time is 1-2 hours；
In the pulverising step, the mesh number of smashed gained herbal cuisine element product is 60-100 mesh；Specially 80 mesh.
4. according to the method in claim 2 or 3, it is characterised in that: the concentrate drying obtains the behaviour of extract dry extract
As: the filtrate decompression is concentrated into thick paste, is transferred in evaporating dish, it is dry, obtain extract dry extract.
5. according to the method described in claim 4, it is characterized by: described be concentrated under reduced pressure at 60-80 DEG C of temperature, vacuum degree-
It is carried out under 0.07~-0.09MPa；The relative density for being concentrated into medicinal extract is 1.2~1.4；
The drying is vacuum drying or oven drying；Drying temperature is specially 55-60 DEG C.
6. according to the method any in claim 2-5, it is characterised in that: the extract dry extract powder is without adjusting
Taste sterilizes after packaging, obtains the herbal cuisine element product；
Alternatively, needing to be added corresponding seasoning according to taste and food additives are seasoned, and shaping auxiliary materials progress is added
Technological forming sterilizes after packaging, obtains the herbal cuisine element product of various tastes；
Wherein, the taste is chosen in particular from fresh perfume, spices, garlic perfume, paste flavor, sour-sweet, sour refreshing, spicy, spicy, vinegar-pepper, curried, extra large
At least one of fresh and tomato；
The herbal cuisine element product can be made into various forms；Specially solid, liquid or lotion.
7. according to the method described in claim 6, it is characterized by: the sterilizing uses irradiation sterilization method；Specially use
The radioactive element Co60 that dosage is 3kGy carries out irradiation sterilization to packaged herbal cuisine element product.
8. application of the herbal cuisine element product in strengthen immunity described in claim 1.
9. application of the herbal cuisine element product described in claim 1 in the product of preparation strengthen immunity.
10. the application method of herbal cuisine element product described in claim 1, comprising: the herbal cuisine element product is direct as seasoning
It is added in vegetable or soup and eats；
Coming-of-Age Day research on maximum utilized quantity is 2g/ times, 3 times/day, is amounted to 6g/ days, wherein people calculates according to 70kg weight.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810757017.XA CN108968066A (en) | 2018-07-11 | 2018-07-11 | A kind of ginseng of strengthen immunity, Rhizoma Atractylodis Macrocephalae, Poria cocos, herbal cuisine element product of fructus lycii and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810757017.XA CN108968066A (en) | 2018-07-11 | 2018-07-11 | A kind of ginseng of strengthen immunity, Rhizoma Atractylodis Macrocephalae, Poria cocos, herbal cuisine element product of fructus lycii and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108968066A true CN108968066A (en) | 2018-12-11 |
Family
ID=64536855
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810757017.XA Pending CN108968066A (en) | 2018-07-11 | 2018-07-11 | A kind of ginseng of strengthen immunity, Rhizoma Atractylodis Macrocephalae, Poria cocos, herbal cuisine element product of fructus lycii and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108968066A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111728216A (en) * | 2020-07-06 | 2020-10-02 | 北京理工亘舒科技有限公司 | Medicated diet vegetarian product and preparation method and application thereof |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1107006A (en) * | 1994-10-08 | 1995-08-23 | 王跃进 | Synthetic gourmet powder |
| CN1565249A (en) * | 2003-07-05 | 2005-01-19 | 张锐 | Chinese medicinal herb nutritious seasoning for cooking |
| CN101002858A (en) * | 2006-12-19 | 2007-07-25 | 戴甲木 | Medicine composition for enhancing immunity, and its preparing method and use |
| CN101401651A (en) * | 2008-10-20 | 2009-04-08 | 宋福兴 | Culinary Chinese medicinal composition |
| CN102273575A (en) * | 2011-08-26 | 2011-12-14 | 吉林紫鑫药业股份有限公司 | Ginseng and tuckahoe cream and production process thereof |
| CN102697845A (en) * | 2010-10-15 | 2012-10-03 | 佳木斯大学 | Compound polysaccharide traditional Chinese medicine microecological regulator |
| CN102823653A (en) * | 2012-09-28 | 2012-12-19 | 广西中医药大学 | Series compound health-care medicated food milk tablets and preparation method thereof |
| CN107802698A (en) * | 2017-12-11 | 2018-03-16 | 吉林亚泰明星制药有限公司 | Chinese medicine composition of strengthen immunity and preparation method thereof |
| CN109498704A (en) * | 2018-12-28 | 2019-03-22 | 中泰宜佳健康科技（北京）有限责任公司 | A kind of raising immunity, the composition for dispelling moisture and its preparation method and application |
-
2018
- 2018-07-11 CN CN201810757017.XA patent/CN108968066A/en active Pending
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1107006A (en) * | 1994-10-08 | 1995-08-23 | 王跃进 | Synthetic gourmet powder |
| CN1565249A (en) * | 2003-07-05 | 2005-01-19 | 张锐 | Chinese medicinal herb nutritious seasoning for cooking |
| CN101002858A (en) * | 2006-12-19 | 2007-07-25 | 戴甲木 | Medicine composition for enhancing immunity, and its preparing method and use |
| CN101401651A (en) * | 2008-10-20 | 2009-04-08 | 宋福兴 | Culinary Chinese medicinal composition |
| CN102697845A (en) * | 2010-10-15 | 2012-10-03 | 佳木斯大学 | Compound polysaccharide traditional Chinese medicine microecological regulator |
| CN102273575A (en) * | 2011-08-26 | 2011-12-14 | 吉林紫鑫药业股份有限公司 | Ginseng and tuckahoe cream and production process thereof |
| CN102823653A (en) * | 2012-09-28 | 2012-12-19 | 广西中医药大学 | Series compound health-care medicated food milk tablets and preparation method thereof |
| CN107802698A (en) * | 2017-12-11 | 2018-03-16 | 吉林亚泰明星制药有限公司 | Chinese medicine composition of strengthen immunity and preparation method thereof |
| CN109498704A (en) * | 2018-12-28 | 2019-03-22 | 中泰宜佳健康科技（北京）有限责任公司 | A kind of raising immunity, the composition for dispelling moisture and its preparation method and application |
Non-Patent Citations (1)
| Title |
|---|
| 刘文杰 等: "中医药对运动免疫功能影响的研究进展", 《四川中医》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111728216A (en) * | 2020-07-06 | 2020-10-02 | 北京理工亘舒科技有限公司 | Medicated diet vegetarian product and preparation method and application thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103355504B (en) | Formula feed for 0-7 weeks-old broiler chickens and preparation method thereof | |
| CN102763786B (en) | Feed capable of improving chicken quality and flavor | |
| CN103734547B (en) | Pig feed for promoting growth of piglets and Chinese medicine additive and preparing method of pig feed and Chinese medicine additive | |
| CN101103799B (en) | Medicated diet stewing in brine, sauce mixing vegetable formula and preparation method thereof | |
| CN110447902B (en) | Composition with weight-losing effect | |
| CN101584469A (en) | Natural spleen-strengthening stomach-nourishing egg and production method thereof | |
| CN103719544B (en) | For improving the feed of meat duck matter | |
| CN103652509A (en) | Steamed bun filled with soup | |
| CN105831594A (en) | Delicious tongue-numbing spicy gluten strip and preparation method thereof | |
| CN106035900A (en) | Ginger tea and preparation method thereof | |
| CN109601978A (en) | A kind of ginseng of strengthen immunity, Radix Angelicae Sinensis, Radix Glycyrrhizae, herbal cuisine element product of Poria cocos and preparation method thereof | |
| CN112546167A (en) | Traditional Chinese medicine composition with spleen invigorating and dampness removing functions and preparation method and application thereof | |
| CN104206853A (en) | Traditional Chinese medicine compound feed for meat quails and preparation method thereof | |
| CN109481589A (en) | A kind of preparation method of the toxin expelling anti-cancer capsule for treating lung cancer and dietotherapy anticancer | |
| KR20160000695A (en) | Herbal-medicine containing dha for recovering fatigue and enhancing memory, and method of manufacturing the same | |
| CN103330175A (en) | Seasoning made of natural plant compositions and preparation method thereof | |
| CN108968066A (en) | A kind of ginseng of strengthen immunity, Rhizoma Atractylodis Macrocephalae, Poria cocos, herbal cuisine element product of fructus lycii and preparation method thereof | |
| CN105994867A (en) | Chocolate with effect of improving immunity and preparation method thereof | |
| CN102652561A (en) | Vegetable protein food for treating micro-disease or assisting medicament in rehabilitating sick body and production method thereof | |
| CN105056103A (en) | Steamed buns with assistant effect of reducing blood fat and cholesterol and processing method of steamed buns | |
| KR101783549B1 (en) | Health supplement food containing dha for recovering fatigue, and method of manufacturing the same | |
| CN113367329A (en) | Three-rice Qinqou meal replacement powder and preparation method thereof | |
| CN105661536A (en) | Nutritional electuary for tonifying kidney and nourishing liver | |
| CN106173128A (en) | A kind of chocolate with anti-fatigue effect and preparation method thereof | |
| CN104585617A (en) | Nutritional red glutinous rice health powder and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20181211 |