CN108949863A - A method of absolute alcohol is produced using corn stover - Google Patents
A method of absolute alcohol is produced using corn stover Download PDFInfo
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- CN108949863A CN108949863A CN201810763687.2A CN201810763687A CN108949863A CN 108949863 A CN108949863 A CN 108949863A CN 201810763687 A CN201810763687 A CN 201810763687A CN 108949863 A CN108949863 A CN 108949863A
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- corn
- absolute alcohol
- corn stover
- saccharification
- liquefied
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/20—Preparation of compounds containing saccharide radicals produced by the action of an exo-1,4 alpha-glucosidase, e.g. dextrose
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/02—Monosaccharides
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/06—Ethanol, i.e. non-beverage
- C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
- C12P7/10—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P2201/00—Pretreatment of cellulosic or lignocellulosic material for subsequent enzymatic treatment or hydrolysis
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
Abstract
The present invention relates to absolute alcohol production technical fields, a kind of method using corn stover production absolute alcohol is specifically disclosed, includes the following steps: S1, by corn straw modification injecting jet liquefied pot, α-amylase is added, injection liquefaction is carried out, liquefied corn stalk is obtained；S2, liquefied corn stalk is sent into saccharifying tank, carbohydrase is added and stirs evenly, is saccharified, obtain saccharification corn stover；S3, into saccharification corn stover, inoculation saccharomyces cerevisiae carries out anaerobic fermentation, obtains fermentation liquid；S4, fermentation liquid is subjected to distilation, obtains absolute alcohol.Working condition of the present invention is mild, and low energy consumption, without secondary pollution, realizes the innoxious of waste, minimizing and recycling.
Description
Technical field
The present invention relates to absolute alcohol production technical fields, and in particular to a kind of to produce absolute alcohol using corn stover
Method.
Background technique
Ethyl alcohol is a kind of important raw material of industry, is widely used in food, chemical industry, medicine and other fields, and can part
Or replacing whole gasoline, have many advantages, such as safe and clean, regeneration.Traditional ethyl alcohol produces mainly with corn, sugarcane, potato etc.
Crops are fermented, and keep its production cost excessively high, are unable to get extensive use.New raw materials for production and technology are had to look for,
The production cost for reducing alcohol fuel, makes it possible its large-scale application.
Summary of the invention
Technical problems based on background technology produce absolute alcohol using corn stover the invention proposes a kind of
Method.The present invention first carries out ultrasonic wave and base extraction to corn stover, then is liquefied, be saccharified, fermented and distilled and nothing is made
Water-alcohol, enzymatic hydrolyzation and sugar yield with higher.
A kind of method using corn stover production absolute alcohol proposed by the present invention, includes the following steps:
S1, by corn straw modification injecting jet liquefied pot, α-amylase is added, carries out injection liquefaction, it is beautiful to obtain liquefaction
Rice stalk；
S2, liquefied corn stalk is sent into saccharifying tank, carbohydrase is added and stirs evenly, is saccharified, it is beautiful to obtain saccharification
Rice stalk；
S3, into saccharification corn stover, inoculation saccharomyces cerevisiae carries out anaerobic fermentation, obtains fermentation liquid；
S4, fermentation liquid is subjected to distilation, obtains absolute alcohol.
Preferably, in S1, corn straw modification the preparation method comprises the following steps: by corn straw smashing, first use the ultrasound of 20-100W
Wave handles 20-45min, and 30-50min is then impregnated in ammonium hydroxide, obtains corn straw modification.
Preferably, in S1, the additional amount of α-amylase is 35-45U/g.
Preferably, in S1, spraying liquefied temperature is 85-95 DEG C, time 1.5-3h.
Preferably, in S2, the additional amount of carbohydrase is 650-750U/g.
Preferably, in S2, the temperature of saccharification is 50-65 DEG C, time 10-15h.
Preferably, in S3, the inoculum concentration of saccharomyces cerevisiae is the 10-15% of saccharification corn stover weight.
Preferably, in S3, the temperature of anaerobic fermentation is 30-40 DEG C, time 65-80h.
The present invention is first modified corn stover, preferably just during extracting absolute alcohol using corn stover
Modified mode is first to carry out ultrasonication in case, since sound wave can generate huge energy by the effect of cavitation, is accelerated
The vibration of lignin destroys lignin, can be improved subsequent liquefaction and saccharification capability, then carry out base extraction, not only may be used
To effectively remove hemicellulose and lignin, liquefaction and saccharification capability are further increased, while the knot of cellulose can be substantially reduced
Brilliant degree improves the efficiency of enzymatic hydrolysis, so that enzymatic hydrolyzation of the invention is 99% or more, sugar yield is 95% or more.The present invention is first right
Corn stover carries out ultrasonic wave and base extraction, then is liquefied, be saccharified, fermented and distilled and absolute alcohol is made, and has higher
Enzymatic hydrolyzation and sugar yield, and working condition of the present invention is mild, and low energy consumption, without secondary pollution, realize waste it is innoxious,
Minimizing and recycling.
Specific embodiment
In the following, technical solution of the present invention is described in detail by specific embodiment.
Embodiment 1
A method of absolute alcohol being produced using corn stover, is included the following steps:
S1, by corn straw modification injecting jet liquefied pot, α-amylase is added, carries out injection liquefaction, it is beautiful to obtain liquefaction
Rice stalk；
S2, liquefied corn stalk is sent into saccharifying tank, carbohydrase is added and stirs evenly, is saccharified, it is beautiful to obtain saccharification
Rice stalk；
S3, into saccharification corn stover, inoculation saccharomyces cerevisiae carries out anaerobic fermentation, obtains fermentation liquid；
S4, fermentation liquid is subjected to distilation, obtains absolute alcohol.
Embodiment 2
A method of absolute alcohol being produced using corn stover, is included the following steps:
S1, by corn straw modification injecting jet liquefied pot, α-amylase is added, carries out injection liquefaction, it is beautiful to obtain liquefaction
Rice stalk；
S2, liquefied corn stalk is sent into saccharifying tank, carbohydrase is added and stirs evenly, is saccharified, it is beautiful to obtain saccharification
Rice stalk；
S3, into saccharification corn stover, inoculation saccharomyces cerevisiae carries out anaerobic fermentation, obtains fermentation liquid；
S4, fermentation liquid is subjected to distilation, obtains absolute alcohol；
Wherein, in S1, corn straw modification the preparation method comprises the following steps: by corn straw smashing, first use the ultrasonication of 40W
30min then impregnates 35min in ammonium hydroxide, obtains corn straw modification.
Embodiment 3
A method of absolute alcohol being produced using corn stover, is included the following steps:
S1, by corn straw modification injecting jet liquefied pot, α-amylase is added, carries out injection liquefaction, it is beautiful to obtain liquefaction
Rice stalk；
S2, liquefied corn stalk is sent into saccharifying tank, carbohydrase is added and stirs evenly, is saccharified, it is beautiful to obtain saccharification
Rice stalk；
S3, into saccharification corn stover, inoculation saccharomyces cerevisiae carries out anaerobic fermentation, obtains fermentation liquid；
S4, fermentation liquid is subjected to distilation, obtains absolute alcohol；
Wherein, in S1, corn straw modification the preparation method comprises the following steps: by corn straw smashing, first use the ultrasonication of 80W
40min then impregnates 45min in ammonium hydroxide, obtains corn straw modification；
In S1, the additional amount of α-amylase is 40U/g；
In S1, spraying liquefied temperature is 90 DEG C, time 2h；
In S2, the additional amount of carbohydrase is 700U/g；
In S2, the temperature of saccharification is 60 DEG C, time 12h；
In S3, the inoculum concentration of saccharomyces cerevisiae is the 13% of saccharification corn stover weight；
In S3, the temperature of anaerobic fermentation is 35 DEG C, time 70h.
Embodiment 4
A method of absolute alcohol being produced using corn stover, is included the following steps:
S1, by corn straw modification injecting jet liquefied pot, α-amylase is added, carries out injection liquefaction, it is beautiful to obtain liquefaction
Rice stalk；
S2, liquefied corn stalk is sent into saccharifying tank, carbohydrase is added and stirs evenly, is saccharified, it is beautiful to obtain saccharification
Rice stalk；
S3, into saccharification corn stover, inoculation saccharomyces cerevisiae carries out anaerobic fermentation, obtains fermentation liquid；
S4, fermentation liquid is subjected to distilation, obtains absolute alcohol；
Wherein, in S1, corn straw modification the preparation method comprises the following steps: by corn straw smashing, first use the ultrasonication of 20W
45min then impregnates 30min in ammonium hydroxide, obtains corn straw modification；
In S1, the additional amount of α-amylase is 45U/g；
In S1, spraying liquefied temperature is 85 DEG C, time 3h；
In S2, the additional amount of carbohydrase is 650U/g；
In S2, the temperature of saccharification is 65 DEG C, time 10h；
In S3, the inoculum concentration of saccharomyces cerevisiae is the 15% of saccharification corn stover weight；
In S3, the temperature of anaerobic fermentation is 30 DEG C, time 80h.
Embodiment 5
A method of absolute alcohol being produced using corn stover, is included the following steps:
S1, by corn straw modification injecting jet liquefied pot, α-amylase is added, carries out injection liquefaction, it is beautiful to obtain liquefaction
Rice stalk；
S2, liquefied corn stalk is sent into saccharifying tank, carbohydrase is added and stirs evenly, is saccharified, it is beautiful to obtain saccharification
Rice stalk；
S3, into saccharification corn stover, inoculation saccharomyces cerevisiae carries out anaerobic fermentation, obtains fermentation liquid；
S4, fermentation liquid is subjected to distilation, obtains absolute alcohol；
Wherein, in S1, corn straw modification the preparation method comprises the following steps: by corn straw smashing, first at the ultrasonic wave of 100W
20min is managed, 50min is then impregnated in ammonium hydroxide, obtains corn straw modification；
In S1, the additional amount of α-amylase is 35U/g；
In S1, spraying liquefied temperature is 95 DEG C, time 1.5h；
In S2, the additional amount of carbohydrase is 750U/g；
In S2, the temperature of saccharification is 50 DEG C, time 15h；
In S3, the inoculum concentration of saccharomyces cerevisiae is the 10% of saccharification corn stover weight；
In S3, the temperature of anaerobic fermentation is 40 DEG C, time 65h.
The foregoing is only a preferred embodiment of the present invention, but scope of protection of the present invention is not limited thereto,
Anyone skilled in the art in the technical scope disclosed by the present invention, according to the technique and scheme of the present invention and its
Inventive concept is subject to equivalent substitution or change, should be covered by the protection scope of the present invention.
Claims (8)
1. a kind of method using corn stover production absolute alcohol, which is characterized in that include the following steps:
S1, by corn straw modification injecting jet liquefied pot, α-amylase is added, carries out injection liquefaction, obtains liquefied corn straw
Stalk；
S2, liquefied corn stalk is sent into saccharifying tank, carbohydrase is added and stirs evenly, is saccharified, obtain saccharification corn stalk
Stalk；
S3, into saccharification corn stover, inoculation saccharomyces cerevisiae carries out anaerobic fermentation, obtains fermentation liquid；
S4, fermentation liquid is subjected to distilation, obtains absolute alcohol.
2. utilizing the method for corn stover production absolute alcohol according to claim 1, which is characterized in that modified beautiful in S1
Rice stalk the preparation method comprises the following steps: by corn straw smashing, the ultrasonication 20-45min of 20-100W is first used, then in ammonium hydroxide
Middle dipping 30-50min, obtains corn straw modification.
3. the method according to claim 1 or claim 2 using corn stover production absolute alcohol, which is characterized in that in S1, liquefaction
The additional amount of enzyme is 35-45U/g.
4. the method according to claim 1 or claim 2 using corn stover production absolute alcohol, which is characterized in that in S1, injection
Liquefied temperature is 85-95 DEG C, time 1.5-3h.
5. the method according to claim 1 or claim 2 using corn stover production absolute alcohol, which is characterized in that in S2, saccharification
The additional amount of enzyme is 650-750U/g.
6. the method according to claim 1 or claim 2 using corn stover production absolute alcohol, which is characterized in that in S2, saccharification
Temperature be 50-65 DEG C, time 10-15h.
7. the method according to claim 1 or claim 2 using corn stover production absolute alcohol, which is characterized in that in S3, wine brewing
The inoculum concentration of yeast is the 10-15% of saccharification corn stover weight.
8. the method according to claim 1 or claim 2 using corn stover production absolute alcohol, which is characterized in that in S3, anaerobism
The temperature of fermentation is 30-40 DEG C, time 65-80h.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201810763687.2A CN108949863A (en) | 2018-07-12 | 2018-07-12 | A method of absolute alcohol is produced using corn stover |
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| CN201810763687.2A CN108949863A (en) | 2018-07-12 | 2018-07-12 | A method of absolute alcohol is produced using corn stover |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101348816A (en) * | 2008-09-13 | 2009-01-21 | 天明（沈阳）酒精有限公司 | Use of low temperature boiling technology in alcohol production |
| CN102191280A (en) * | 2011-03-04 | 2011-09-21 | 北京林业大学 | Method for preparing ethanol from furfural dregs and corn through co-fermentation |
| CN107974467A (en) * | 2017-12-06 | 2018-05-01 | 广东中科天元新能源科技有限公司 | A kind of technique that alcohol fuel is produced as raw material using starchiness and lignocellulosic |
| CN107988273A (en) * | 2017-11-29 | 2018-05-04 | 广西中粮生物质能源有限公司 | A kind of method for controlling rice liquefaction DE values |
| CN108165585A (en) * | 2018-02-08 | 2018-06-15 | 江苏金茂源生物化工有限责任公司 | Alcohol fermentation method |
-
2018
- 2018-07-12 CN CN201810763687.2A patent/CN108949863A/en not_active Withdrawn
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101348816A (en) * | 2008-09-13 | 2009-01-21 | 天明（沈阳）酒精有限公司 | Use of low temperature boiling technology in alcohol production |
| CN102191280A (en) * | 2011-03-04 | 2011-09-21 | 北京林业大学 | Method for preparing ethanol from furfural dregs and corn through co-fermentation |
| CN107988273A (en) * | 2017-11-29 | 2018-05-04 | 广西中粮生物质能源有限公司 | A kind of method for controlling rice liquefaction DE values |
| CN107974467A (en) * | 2017-12-06 | 2018-05-01 | 广东中科天元新能源科技有限公司 | A kind of technique that alcohol fuel is produced as raw material using starchiness and lignocellulosic |
| CN108165585A (en) * | 2018-02-08 | 2018-06-15 | 江苏金茂源生物化工有限责任公司 | Alcohol fermentation method |
Non-Patent Citations (3)
| Title |
|---|
| 中国可再生能源学会编: "《可再生能源与低碳社会》", 31 January 2016, 中国科学技术出版社 * |
| 李忠正主编: "《植物纤维资源化学》", 30 June 2012, 中国轻工业出版社 * |
| 杨东方，苗振清编著: "《数学模型在生态学的应用及研究》", 30 November 2013, 海洋出版社 * |
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Application publication date: 20181207 |